DOCSLIB.ORG

Psychrobacter Submarinus Sp. Nov. and Psychrobacter Marincola Sp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Psychrobacter Submarinus Sp. Nov. and Psychrobacter Marincola Sp International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1291–1297 DOI: 10.1099/ijs.0.02087-0 Psychrobacter submarinus sp. nov. and NOTE Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments 1 Pacific Institute of Lyudmila A. Romanenko,1 Peter Schumann,2 Manfred Rohde,3 Bioorganic Chemistry, 4 1 2 Far-Eastern Branch, Anatoly M. Lysenko, Valery V. Mikhailov and Erko Stackebrandt Russian Academy of Sciences, 690022 Vladivostok, Prospekt 100 Author for correspondence: Erko Stackebrandt. Tel: j49 531 2616 352. Fax: j49 531 2616 418. Let Vladivostoku, 159, e-mail: erko!dsmz.de Russia 2,3 DSMZ – Deutsche Sammlung von Two novel psychrophilic, halophilic, Psychrobacter-like bacteria, strains KMM Mikroorganismen und 225T and KMM 277T, were isolated from sea water and the internal tissues of an 2 Zellkulturen GmbH and ascidian Polysyncraton sp. specimen, respectively, and characterized using a GBF – Gesellschaft fu$ r Biotechnologische polyphasic approach, which included phenotypic, genotypic, chemotaxonomic Forschung GmbH3 , and phylogenetic analyses. The novel marine isolates were Gram-negative, D-38124 Braunschweig, aerobic, coccoid, oxidase- and catalase-positive, non-pigmented, non-motile, Germany psychrophilic and halophilic and they utilized a restricted spectrum of carbon 4 Institute of Microbiology, sources. Strains KMM 225T and KMM 277T required sea water or sodium ions for Russian Academy of Sciences, 117811 Moscow, growth and were tolerant of up to 12–15% (w/v) NaCl. Growth of strains KMM Russia 225T and KMM 277T was observed at 4–35 and 7–35 SC, respectively. The DNA GMC contents of KMM 225T and KMM 277T were respectively 468 and 507 mol%. Comparison of almost complete 16S rDNA sequences of strains KMM 225T and KMM 277T revealed that both strains were phylogenetically most closely related to each other (999% sequence similarity) and slightly less related to Psychrobacter glacincola, with 972 and 978% similarity, respectively. DNA–DNA reassociation between KMM 225T and KMM 277T revealed 15% similarity, whereas similarity to other Psychrobacter species was 14–25%. Strains KMM 225T and KMM 277T differed from one another in their growth temperature, organic substrate utilization, antibiotic sensitivity and DNA GMC content. Both strains examined could be distinguished from all previously described Psychrobacter species by their physiological, genotypic and phylogenetic characteristics. On the basis of the physiological and molecular properties of the novel isolates, the names Psychrobacter submarinus sp. nov. (type strain KMM 225T l DSM 14161T) and Psychrobacter marincola sp. nov. (type strain KMM 277T l DSM 14160T) are proposed. Keywords: Psychrobacter submarinus sp. nov., Psychrobacter marincola sp. nov., psychrophilic, halophilic bacteria, marine environments Gram-negative, aerobic, non-motile, psychrophilic, tive, non-motile, psychrophilic or psychrotolerant, halotolerant micro-organisms are common inhabi- halotolerant bacteria (Juni & Heym, 1986). DNA– tants of terrestrial and marine environments, including rRNA hybridization analysis performed by Rossau et the Antarctic and deep oceanic environments. The al. (1991) showed that this genus belongs to the family genus Psychrobacter was described to accommodate Moraxellaceae, which also includes the genera Gram-negative, moraxella-like, coccoid, oxidase-posi- Moraxella and Acinetobacter (Rossau et al., 1991). Members of the genus Psychrobacter have been iso- ................................................................................................................................................. lated from a wide range of habitats including sea The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences water, the gills and skin of fish, poultry, food and of strains KMM 225T and KMM 227T are respectively AJ309940 and clinical sources (Juni, 1991). Recently, three Antarctic AJ309941. Psychrobacter species with low growth-temperature 02087 # 2002 IUMS Printed in Great Britain 1291 L. A. Romanenko and others optima were described, Psychrobacter urativorans and Psychrobacter frigidicola, both from Antarctic ornitho- genic soils, and Psychrobacter glacincola from Ant- arctic sea ice (Bowman et al., 1996, 1997), indicating that members of the genus Psychrobacter are wide- spread in cold Antarctic environments. The novel species Psychrobacter pacificensis, which differs from known terrestrial and Antarctic species, was described for psychrophilic strains isolated from deep sea water (5000–6000 m) of the Japan Trench (Maruyama et al., 2000). In the present study, the taxonomic position of two strains, KMM 225T and KMM 227T, from marine environments was defined. Results revealed that these bacteria represent two novel species of the genus Psychrobacter, for which the names Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov. are proposed. Isolation, cultural characteristics and morphology Strain KMM 225T was isolated from a sea-water sample obtained at a depth of 300 m in the north- western part of the Pacific Ocean (12m 41h N, 132m 38h W) in July 1985. The sea-water sample was taken with a plastic hydrological bathometer. Strain KMM 277T was isolated from the homogenized internal tissues of the ascidian Polysyncraton sp., collected in the Indian Ocean (33m 41h S, 134m 28h E) in March 1988. The homogenate was prepared and diluted in sterile sea water. Aliquots of the sea-water sample and the diluted homogenate were spread on agar plates of sea-water medium (SWM), which was made up of 750 ml sea water, 250 ml distilled water and the following com- −" ponents (g l ): peptone (5n0), yeast extract (2n5), glucose (1n0), K#HPO% (0n2), MgSO% (0n05) and agar (15n0). Samples were incubated for 7–10 days at 28 mC. Isolates were cultivated aerobically on SWM, 2216 marine agar (MA) or marine broth (MB) (Difco) at 25–28 mC. Bacteria were stored at k80 mCin30% (v\v) glycerol. Isolates designated KMM 225T and T KMM 277 have been deposited in the Collection of ................................................................................................................................................. Marine Micro-organisms (KMM) of the Pacific In- Fig. 1. Transmission electron micrographs showing general stitute of Bioorganic Chemistry, Vladivostok, Russia. morphology of negatively stained cells of Psychrobacter sub- marinus KMM 225T (a, b) and Psychrobacter marincola KMM For determination of biochemical and cultural tests, 277T (c). Bars, 1 (a), 0 5 (b) and 0 25 (c) µm. except those used for determination of the temperature n n range for growth, Psychrobacter immobilis CIP 102557T, P. urativorans CIP 105100T and P. frigidicola CIP 105101T were cultivated on trypticase soy agar (TSA; Difco) at 28, 20 and 15 mC, respectively. uranyl acetate and carbon film. Samples were Psychrobacter phenylpyruvicus CIP 82.27T was grown examined with a Zeiss transmission electron micro- on brain\heart infusion agar (BHIA; Difco) supple- scope TEM910 at an acceleration voltage of 80 kV at mented with 5% (v\v) blood serum at 30 mC and P. calibrated magnifications. Motility was observed with glacincola CIP 105313T was grown on MA 2216 at a light microscope by the hanging-drop method. 15 mC. The strains studied were slightly different in mor- Cell morphology was examined by transmission elec- phology and cell dimensions. Whereas cells of KMM T tron microscopy of exponential-phase cells grown in 225 were ovoid, 1n6–1n9 µm long and 0n7–1n0 µmin MB 2216. Cells were fixed with 1% (v\v) glutaral- diameter (Fig. 1a, b), those of KMM 277T were dehyde and negatively stained with 4% (w\v) aqueous coccoid, 0n7–1n0 µm in diameter (Fig. 1c). 1292 International Journal of Systematic and Evolutionary Microbiology 52 Two novel Psychrobacter species Growth characteristics and metabolic properties reduction, tryptophan deaminase, phenylalanine deaminase, the ONPG test, Simmons’ citrate and The Gram-reaction, oxidase, catalase, production of Voges–Proskauer tests, H S production, denitri- caseinase, deoxyribonuclease, gelatinase and lipase # fication, acid production from -glucose, -xylose, (Tween 80) were tested according to the standard rhamnose, galactose, maltose, lactose and mannitol methods described by Smibert & Krieg (1994) using and utilization of citrate, -malate, lactate, acetate, SWM as the basal medium. Amylase activity was propionate, -histidine, -proline, -alanine and n- observed on nutrient medium supplemented with valerate. starch at a concentration of 10% (w\v) by flooding plates with 1% (w\v) iodine solution. The ability to grow at different temperatures was tested on MA and Chemotaxonomy TSA at 4, 7, 10, 15, 25, 30, 35, 37 and 40 mC. The pH Cellular fatty acid methyl esters obtained from cells range for growth (5n0–10n0) was tested using MB with grown in MB at 28 mC by the method of Stead et al. pH values adjusted by the addition of 5 M NaOH or (1992) were separated by GC (Groth et al., 1996) and HCl. Sodium ion requirement and tolerance of NaCl identified as described previously (Schumann et al., were examined on SWM prepared with the artificial 1997). Polar lipids extracted by the method of sea-water base adjusted so that the amount of NaCl Minnikin et al. (1979) were identified by two- was between 0 and 20% (w\v). Oxidative utilization of dimensional TLC and spraying with specific reagents glucose was determined by the method of Leifson (Collins & Jones, 1980). The novel isolates possessed (1963). Leifson’s medium was also used for testing acid
Load more

Recommended publications
  • Pseudomonas Spp. and Other Psychrotrophic
    Pesq. Vet. Bras. 39(10):807-815, October 2019 DOI: 10.1590/1678-5150-PVB-6037 Original Article Livestock Diseases ISSN 0100-736X (Print) ISSN 1678-5150 (Online) PVB-6037 LD Pseudomonas spp. and other psychrotrophic microorganisms in inspected and non-inspected Brazilian Minas Frescal cheese: proteolytic, lipolytic and AprX production potential1 Pedro I. Teider Junior2, José C. Ribeiro Júnior3* , Eric H. Ossugui2, Ronaldo Tamanini2, Juliane Ribeiro2, Gislaine A. Santos2 2 and Vanerli Beloti2 , Amauri A. Alfieri ABSTRACT.- Teider Junior P.I., Ribeiro Júnior J.C., Ossugui E.H., Tamanini R., Ribeiro J., Santos G.A., Pseudomonas spp. and other psychrotrophic microorganisms Pseudomonas spp. and other psychrotrophic in inspected and non-inspected Brazilian Minas Frescal cheese: proteolytic, lipolytic andAlfieri AprX A.A. production& Beloti V. 2019. potential . Pesquisa Veterinária Brasileira 39(10):807-815. Instituto microorganisms in inspected and non- Nacional de Ciência e Tecnologia para a Cadeia Produtiva do Leite, Universidade Estadual de inspected Brazilian Minas Frescal cheese: Londrina, Rodovia Celso Garcia Cid PR-445 Km 380, Cx. Postal 10.011, Campus Universitário, proteolytic, lipolytic and AprX production Londrina, PR 86057-970, Brazil. E-mail: [email protected] The most consumed cheese in Brazil, Minas Frescal cheese (MFC) is highly susceptible to potential microbial contamination and clandestine production and commercialization can pose a risk to consumer health. The storage of this fresh product under refrigeration, although more appropriate, may favor the growth of spoilage psychrotrophic bacteria. The objective of this [Pseudomonas spp. e outros micro-organismos study was to quantify and compare Pseudomonas spp. and other psychrotrophic bacteria in inspected and non-inspected MFC samples, evaluate their lipolytic and proteolytic activities and e não inspecionados: potencial proteolítico, lipolítico e their metalloprotease production potentials.
    [Show full text]
  • Growth and Adaptation of Microorganisms on the Cheese Surface Christophe Monnet, Sophie Landaud-Liautaud, Pascal Bonnarme, Dominique Swennen
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Archive Ouverte en Sciences de l'Information et de la Communication Growth and adaptation of microorganisms on the cheese surface Christophe Monnet, Sophie Landaud-Liautaud, Pascal Bonnarme, Dominique Swennen To cite this version: Christophe Monnet, Sophie Landaud-Liautaud, Pascal Bonnarme, Dominique Swennen. Growth and adaptation of microorganisms on the cheese surface. FEMS Microbiology Letters, Wiley-Blackwell, 2015, 362 (1), pp.1-9. 10.1093/femsle/fnu025. hal-01535275 HAL Id: hal-01535275 https://hal.archives-ouvertes.fr/hal-01535275 Submitted on 28 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 Growth and adaptation of microorganisms on the cheese surface 2 3 4 Christophe Monnet1,2, Sophie Landaud2,1, Pascal Bonnarme1,2 & Dominique Swennen3,4 5 6 1 INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, 78370 Thiverval- 7 Grignon, France 8 2 AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, 78370 9 Thiverval-Grignon, France 10 3 INRA, UMR1319 Micalis, 78370 Thiverval-Grignon, France 11 4 AgroParisTech, UMR1319 Micalis, 78370 Thiverval-Grignon, France 12 13 * Corresponding author.
    [Show full text]
  • Compositional and Functional Comparisons of the Microbiota in the Colostrum and Mature Milk of Dairy Goats
    Compositional and functional comparisons of the microbiota in the colostrum and mature milk of dairy goats Zhannur Niyazbekova Northwest Agriculture and Forestry University Xiao-Ting Yao Northwest Agriculture and Forestry University Ming-Jie Liu Northwest Agriculture and Forestry University Nomin Bold Northwest Agriculture and Forestry University Juan-Zhen Tong Northwest Agriculture and Forestry University Jian-Jun Chang Qinghai University Ying Wen Qinghai University Li Li Qinghai University Yong Wang Qinghai University De-Kun Chen Northwest Agriculture and Forestry University Wen-Tao Ma ( [email protected] ) Northwest Agriculture and Forestry University https://orcid.org/0000-0002-4747-1489 Research article Keywords: Goat milk, Colostrum, Mature milk, Milk microbiota, Microbial diversity Posted Date: May 12th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-26653/v1 Page 1/20 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 2/20 Abstract Background Goat milk is essential for the initial development of kids by providing a great source of commensal bacteria. Here we analyzed the microbiota of goat colostrum which was collected daily for ve days post delivery and mature milk collected at the 7th, 10th, 20nd, 30th, 40th, 50th and 60th days, respectively, from three farms of Shaanxi province. Results The result showed that microbial alpha diversity was higher in the mature milk compared with that in the colostrum. According to taxonomy results, Proteobacteria, Firmicutes,
    [Show full text]
  • Humpback Whale Populations Share a Core Skin Bacterial Community: Towards a Health Index for Marine Mammals?
    Humpback Whale Populations Share a Core Skin Bacterial Community: Towards a Health Index for Marine Mammals? Amy Apprill1*, Jooke Robbins2, A. Murat Eren3, Adam A. Pack4,5, Julie Reveillaud3, David Mattila6, Michael Moore1, Misty Niemeyer7, Kathleen M. T. Moore7, Tracy J. Mincer1* 1 Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, United States of America, 2 Center for Coastal Studies, Provincetown, Massachusetts, United States of America, 3 Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America, 4 University of Hawaii at Hilo, Hilo, Hawaii, United States of America, 5 The Dolphin Institute, Honolulu, Hawaii, United States of America, 6 Hawaiian Islands Humpback Whale National Marine Sanctuary, Kihei, Hawaii, United States of America, 7 International Fund for Animal Welfare, Yarmouth Port, Massachusetts, United States of America Abstract Microbes are now well regarded for their important role in mammalian health. The microbiology of skin – a unique interface between the host and environment - is a major research focus in human health and skin disorders, but is less explored in other mammals. Here, we report on a cross-population study of the skin-associated bacterial community of humpback whales (Megaptera novaeangliae), and examine the potential for a core bacterial community and its variability with host (endogenous) or geographic/environmental (exogenous) specific factors. Skin biopsies or freshly sloughed skin from 56 individuals were sampled from populations in the North Atlantic, North Pacific and South Pacific oceans and bacteria were characterized using 454 pyrosequencing of SSU rRNA genes. Phylogenetic and statistical analyses revealed the ubiquity and abundance of bacteria belonging to the Flavobacteria genus Tenacibaculum and the Gammaproteobacteria genus Psychrobacter across the whale populations.
    [Show full text]
  • Psychrobacter Arenosus Bacteremia After Blood Transfusion, France
    DISPATCHES rapidly increased to 40°C) and had chills and headache. Psychrobacter The transfusion was stopped and the patient transferred to the Department of Internal Medicine. At examination, there arenosus was no hypotension, jaundice, or red urine. Standard laboratory testing showed no ABO Bacteremia incompatibility, hemoglobinemia, hemoglobinuria, and coagulation disorders. According to recommendations after Blood of the Agence Nationale de Sécurité du Médicament Transfusion, (Saint-Denis, France), 3 sets of aerobic and anaerobic blood cultures (Bactec; Becton Dickinson, Pont de Clay, France France) for the recipient (1 immediately and 2 others 4 hours later) and the remaining part of the third erythrocyte Yvan Caspar, Christine Recule, Patricia Pouzol, unit were sent to the bacteriology laboratory for culture. Bruno Lafeuillade, Marie-Reine Mallaret, Gram staining of a blood smear prepared from the third Max Maurin, and Jacques Croize erythrocyte unit showed a large number (≈106 CFU/mL) of gram-variable coccobacilli. We report a case of transfusion-associated bactere- Samples were placed on Columbia blood agar mia caused by Psychrobacter arenosus. This psychrotoler- ant bacterium was previously isolated in 2004 from coastal (bioMérieux, Marcy L’Etoile, France) and incubated at sea ice and sediments in the Sea of Japan, but not from 37°C in anaerobic or 5% CO2–enriched atmospheres. humans. P. arenosus should be considered a psychrotoler- Sample inoculated into blood culture bottles were ant bacterial species that can cause transfusion-transmitted incubated at 37°C under aerobic and anaerobic conditions bacterial infections. (Figure). The aerobic blood culture bottle of the first sample obtained from the recipient and aerobic cultures of the third erythrocyte unit enabled isolation of the same acteria are the leading cause of transfusion-transmitted gram-variable coccobacilli after incubation for 48 hours infections (1).
    [Show full text]
  • Fish Bacterial Flora Identification Via Rapid Cellular Fatty Acid Analysis
    Fish bacterial flora identification via rapid cellular fatty acid analysis Item Type Thesis Authors Morey, Amit Download date 09/10/2021 08:41:29 Link to Item http://hdl.handle.net/11122/4939 FISH BACTERIAL FLORA IDENTIFICATION VIA RAPID CELLULAR FATTY ACID ANALYSIS By Amit Morey /V RECOMMENDED: $ Advisory Committe/ Chair < r Head, Interdisciplinary iProgram in Seafood Science and Nutrition /-■ x ? APPROVED: Dean, SchooLof Fisheries and Ocfcan Sciences de3n of the Graduate School Date FISH BACTERIAL FLORA IDENTIFICATION VIA RAPID CELLULAR FATTY ACID ANALYSIS A THESIS Presented to the Faculty of the University of Alaska Fairbanks in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE By Amit Morey, M.F.Sc. Fairbanks, Alaska h r A Q t ■ ^% 0 /v AlA s ((0 August 2007 ^>c0^b Abstract Seafood quality can be assessed by determining the bacterial load and flora composition, although classical taxonomic methods are time-consuming and subjective to interpretation bias. A two-prong approach was used to assess a commercially available microbial identification system: confirmation of known cultures and fish spoilage experiments to isolate unknowns for identification. Bacterial isolates from the Fishery Industrial Technology Center Culture Collection (FITCCC) and the American Type Culture Collection (ATCC) were used to test the identification ability of the Sherlock Microbial Identification System (MIS). Twelve ATCC and 21 FITCCC strains were identified to species with the exception of Pseudomonas fluorescens and P. putida which could not be distinguished by cellular fatty acid analysis. The bacterial flora changes that occurred in iced Alaska pink salmon ( Oncorhynchus gorbuscha) were determined by the rapid method.
    [Show full text]
  • Active Microorganisms Thrive Among Extremely Diverse Communities in Cloud Water Pierre Amato, Muriel Joly, Ludovic Besaury, Anne Oudart, Najwa Taïb, Anne I
    Active microorganisms thrive among extremely diverse communities in cloud water Pierre Amato, Muriel Joly, Ludovic Besaury, Anne Oudart, Najwa Taïb, Anne I. Moné, Laurent Deguillaume, Anne-Marie Delort, Didier Debroas To cite this version: Pierre Amato, Muriel Joly, Ludovic Besaury, Anne Oudart, Najwa Taïb, et al.. Active microorganisms thrive among extremely diverse communities in cloud water. PLoS ONE, Public Library of Science, 2017, 12 (8), 10.1371/journal.pone.0182869. hal-01598571 HAL Id: hal-01598571 https://hal.archives-ouvertes.fr/hal-01598571 Submitted on 6 Nov 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. RESEARCH ARTICLE Active microorganisms thrive among extremely diverse communities in cloud water Pierre Amato1*, Muriel Joly1, Ludovic Besaury1, Anne Oudart1,2, Najwa Taib2, Anne I. MoneÂ2, Laurent Deguillaume3, Anne-Marie Delort1, Didier Debroas2 1 Universite Clermont Auvergne, CNRS, Institut de Chimie de Clermont-Ferrand, Clermont-Ferrand, France, 2 Universite Clermont Auvergne, CNRS, Laboratoire Microorganismes: GeÂnome et Environnement, Clermont-Ferrand, France, 3 Universite Clermont Auvergne, CNRS, Observatoire de Physique du Globe, Clermont-Ferrand, France a1111111111 a1111111111 * [email protected] a1111111111 a1111111111 a1111111111 Abstract Clouds are key components in Earth's functioning.
    [Show full text]
  • Marine Drugs
    Mar. Drugs 2015, 13, 4539-4555; doi:10.3390/md13074539 OPEN ACCESS marine drugs ISSN 1660-3397 www.mdpi.com/journal/marinedrugs Article Structural Investigation of the Oligosaccharide Portion Isolated from the Lipooligosaccharide of the Permafrost Psychrophile Psychrobacter arcticus 273-4 Angela Casillo 1, Ermenegilda Parrilli 1, Sannino Filomena 1,2, Buko Lindner 3, Rosa Lanzetta 1, Michelangelo Parrilli 4, Maria Luisa Tutino 1 and Maria Michela Corsaro 1,* 1 Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, Napoli 80126, Italy; E-Mails: [email protected] (A.C.); [email protected] (E.P.); [email protected] (S.F.); [email protected] (R.L.); [email protected] (M.L.T.) 2 Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, Napoli 80131, Italy 3 Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, BorstelD-23845, Germany; E-Mail: [email protected] 4 Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, Napoli 80126, Italy; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +39-081-674149; Fax: +39-081-674393. Academic Editor: Antonio Trincone Received: 22 June 2015 / Accepted: 14 July 2015 /Published: 22 July 2015 Abstract: Psychrophilic microorganisms have successfully colonized all permanently cold environments from the deep sea to mountain and polar regions. The ability of an organism to survive and grow in cryoenviroments depends on a number of adaptive strategies aimed at maintaining vital cellular functions at subzero temperatures, which include the structural modifications of the membrane.
    [Show full text]
  • Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H2O2 Environments Isao Yumoto, Yoshiko Hanaoka and Isao Hara
    Chapter Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H2O2 Environments Isao Yumoto, Yoshiko Hanaoka and Isao Hara Abstract Enzymatic evolutionary strategies for adaptation to a high H2O2 environment have been evaluated using catalases with high catalytic efficiency isolated from two H2O2-tolerant bacteria, Exiguobacterium oxidotolerans and Psychrobacter piscatori. The entrance size of the narrow main channel in catalase has been estimated by deter- mining the formation rate of the intermediate state of peracetic acid (b), which is a larger substrate than H2O2 versus that of catalase activity with H2O2 (a) (calculated as b/a). The ratio of b/a in E. oxidotolerans catalase (EKTA) is much higher than that of P. piscatori catalase (PKTA). To elucidate the structural differences between the catalases, the amino acids present in the main channel have been compared between the two catalases and other catalases in the database. The combination of amino acid residues, which contribute high catalytic efficiency in the narrow main chan- nel of EKTA were different from those in PKTA. In this review, we discuss strategic differences in the elimination of high concentration of H2O2 owing to differences in the phylogenetic positions of catalases. In addition, we describe the relationships between the environmental distributions of genera involved in H2O2-resistant bacte- ria and their catalase functions based on the main channel structure of catalase. Keywords: H2O2-tolerant bacteria, Exiguobacterium, Psychrobacter, Vibrio, catalase, narrow main channel, bottleneck size 1. Introduction Oxygen is important for metabolism, acting as a terminal electron acceptor in aerobic bacteria, and these bacteria produce intracellular reactive oxygen species 2•− (ROS), such as hydrogen peroxide (H2O2), superoxide (O ), and hydroxyl radical • (OH ) as by-products of oxygen metabolism [1–4].
    [Show full text]
  • The Microbiome of North Sea Copepods
    Helgol Mar Res (2013) 67:757–773 DOI 10.1007/s10152-013-0361-4 ORIGINAL ARTICLE The microbiome of North Sea copepods G. Gerdts • P. Brandt • K. Kreisel • M. Boersma • K. L. Schoo • A. Wichels Received: 5 March 2013 / Accepted: 29 May 2013 / Published online: 29 June 2013 Ó Springer-Verlag Berlin Heidelberg and AWI 2013 Abstract Copepods can be associated with different kinds Keywords Bacterial community Á Copepod Á and different numbers of bacteria. This was already shown in Helgoland roads Á North Sea the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community Introduction of four frequently occurring copepod species, Acartia sp., Temora longicornis, Centropages sp. and Calanus helgo- Marine copepods may constitute up to 80 % of the meso- landicus from Helgoland Roads (North Sea) over a period of zooplankton biomass (Verity and Smetacek 1996). They are 2 years using DGGE (denaturing gradient gel electrophore- key components of the food web as grazers of primary pro- sis) and subsequent sequencing of 16S-rDNA fragments. To duction and as food for higher trophic levels, such as fish complement the PCR-DGGE analyses, clone libraries of (Cushing 1989; Møller and Nielsen 2001). Copepods con- copepod samples from June 2007 to 208 were generated. tribute to the microbial loop (Azam et al. 1983) due to Based on the DGGE banding patterns of the two years sur- ‘‘sloppy feeding’’ (Møller and Nielsen 2001) and the release vey, we found no significant differences between the com- of nutrients and DOM from faecal pellets (Hasegawa et al.
    [Show full text]
  • Structure of the O‑Specific Polysaccharide from The
    Note pubs.acs.org/jnp Structure of the O‑Specific Polysaccharide from the Lipopolysaccharide of Psychrobacter cryohalolentis K5T Containing a 2,3,4-Triacetamido-2,3,4-trideoxy‑L‑arabinose Moiety † ‡ † § # Anna N. Kondakova,*, Kseniya A. Novototskaya-Vlasova, Nikolay P. Arbatsky, Marina S. Drutskaya, , ⊥ † ‡ § # Victoria A. Shcherbakova, Alexander S. Shashkov, David A. Gilichinsky, Sergei A. Nedospasov, , † and Yuriy A. Knirel † Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia ‡ Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, 142290 Pushchino, Russia § Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia # Faculty of Biology, Moscow State University, 119991 Moscow, Russia ⊥ Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia ABSTRACT: A novel constituent of bacterial polysaccharides, 2,3,4-triacetamido-2,3,4-trideoxy-L-arabinose, was found in the O-specific polysaccharide from the lipopolysaccharide of Psychrobacter cryohalolentis K5T and identified by 1D and 2D 1H and 13C NMR studies of the polysaccharide and a disaccharide obtained by solvolysis of the polysaccharide with triflic acid. The following structure of the branched polysaccharide was established by sugar analysis, triflic acid solvolysis, Smith degradation, and 2D NMR spectroscopy. Psychrobacter is a genus within the family Moraxellaceae of arabinose, which has not been hitherto reported in natural gamma-proteobacteria, which comprises psychrophilic to carbohydrates. mesophilic, halotolerant, aerobic, nonmotile, Gram-negative The lipopolysaccharide was obtained from dried bacterial coccobacilli.1 These bacteria live in extremely cold habitats, cells by the phenol−water procedure8 and degraded under mild such as Antarctic ice, soil, and sediments, as well as in deep sea acidic conditions.
    [Show full text]
  • Rebecca Olivia Maclennan Cowie a Thesis Submitted to Victoria
    BACTERIAL COMMUNITY STRUCTURE, FUNCTION AND DIVERSITY IN ANTARCTIC SEA ICE Rebecca Olivia MacLennan Cowie A thesis submitted to Victoria University of Wellington in fulfillment of the requirement for the degree of Doctor of Philosophy in Ecology & Biodiversity Victoria University of Wellington Te Whare Wananga o te Upoko o te Ika a Maui 2011 “I make no apologies for putting microorganisms on a pedestal above all other living things. For if the last blue whale choked to death on the last panda, it would be disastrous but not the end of the world. But if we accidentally poisoned the last two species of ammonia-oxidisers, that would be another matter. It could be happening now and we wouldn’t even know...” Tom Curtis (July 2006) ACKNOWLEDGEMENTS I would first like to thank my supervisors, Ken Ryan and Els Maas, for without them this thesis would not have been possible. Ken, thank you for everything! Thanks for giving me the opportunity to carry out research as part of the K043 team in Antarctica. I am grateful for the wealth of time you had for me whenever I came knocking on your door. I appreciate for your support, time and effort throughout the last three years and especially during the write-up. Els, thank you for giving so much of your time and energy towards my research. Your knowledge and advice has been invaluable. To my friends, fellow students and office mates who helped me along the way both scientifically and recreationally I thank Bionda Morelissen, Alejandra Perea Blazquez, Charles Lee, David Weller, Abi Powell, Mareike Sudek, Ingrid Knapp and Jade Berman.
    [Show full text]