Supplementary Information for Earth history and the passerine superradiation Oliveros, Carl H., Daniel J. Field, Daniel T. Ksepka, F. Keith Barker, Alexandre Aleixo, Michael J. Andersen, Per Alström, Brett W. Benz, Edward L. Braun, Michael J. Braun, Gustavo A. Bravo, Robb T. Brumfield, R. Terry Chesser, Santiago Claramunt, Joel Cracraft, Andrés M. Cuervo, Elizabeth P. Derryberry, Travis C. Glenn, Michael G. Harvey, Peter A. Hosner, Leo Joseph, Rebecca Kimball, Andrew L. Mack, Colin M. Miskelly, A. Townsend Peterson, Mark B. Robbins, Frederick H. Sheldon, Luís Fábio Silveira, Brian T. Smith, Noor D. White, Robert G. Moyle, Brant C. Faircloth Corresponding authors: Carl H. Oliveros, Email:
[email protected] Brant C. Faircloth, Email:
[email protected] This PDF file includes: Supplementary text Figs. S1 to S10 Table S1 to S3 References for SI reference citations Other supplementary materials for this manuscript include the following: Supplementary Files S1 to S3 1 www.pnas.org/cgi/doi/10.1073/pnas.1813206116 Supplementary Information Text Extended Materials and Methods Library preparation and sequencing. We extracted and purified DNA from fresh muscle tissue, liver tissue, or toepad clips from 113 vouchered museum specimens (Supplementary File S1) using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer’s protocol. We quantified DNA extracts using a Qubit fluorometer, and we prepared aliquots of DNA extracted from muscle and liver at 10 ng/µL in 60 µL volume for shearing. We sheared each DNA sample to 400–600 bp using a Qsonica Q800R sonicator for 15–45 cycles, with each cycle running for 20 seconds on and 20 seconds off at 25% amplitude.