Investigating synergism within multimodular glycoside hydrolases during wheat straw cell wall deconstruction Cédric Montanier, Louise Badruna, Thierry Vernet, Anne-Marie Di Guilmi, Vincent Burlat, Michael O’Donohue To cite this version: Cédric Montanier, Louise Badruna, Thierry Vernet, Anne-Marie Di Guilmi, Vincent Burlat, et al.. Investigating synergism within multimodular glycoside hydrolases during wheat straw cell wall decon- struction. The CBM11 - 11. Carbohydrate Bioengineering Meeting, May 2015, Espoo, Finland. 223 p., 2015, 11. Carbohydrate Bioengineering Meeting. hal-01269244 HAL Id: hal-01269244 https://hal.archives-ouvertes.fr/hal-01269244 Submitted on 3 Jun 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Welcome to CBM11 The eleventh Carbohydrate Bioengineering Meeting (CBM11) focuses on various aspects of carbohydrate-acting enzymes and biomolecules, their mode of action, structure and structure function relationships, engineering and applications. The applications spread from health and nutrition to material sciences. The subjects are highly topical in the present world, where sustainable use of renewables is a key interest, not just for scientists, but for the whole society, governments and industries. Thanks to the contributions of the participants we have been able to design a programme which covers the recent developments in the focus areas of the meeting. The present programme in Espoo includes 55 talks and flash presentations and 133 poster presentations. The roots of CBM11 go back to Helsingør, Denmark and 1995, when the first CBM meeting was organized twenty years ago. The major aim of the meeting is again to bring together colleagues and scientists active in various fields of carbohydrates, biomolecules and carbohydrate-active enzymes. Our target has been to generate a pleasant environment for knowledge sharing, generating collaborations, and hopefully also for constituting an incubator for many future project ideas. The meeting takes place in Otaniemi, in a beautiful natural cape of the Baltic Sea in Espoo, the home of Aalto University engineering schools and VTT. The programme runs in Dipoli, a famous conference building designed in early 1960´s by Raili and Reima Pietilä showing the interplay of light, Finnish pine wood, copper, and natural rocks. If you are interested in architecture, you may want to take a walk in the campus area, which is designed by the most famous Finnish architect Alvar Aalto and to stop by in the main building of Aalto University and the main library, both from 1960’s. On the behalf of the local Organizing Committee we would like to express our gratitude to all our sponsor companies and to all persons who have contributed to organizing this meeting. We wish you a fruitful meeting of scientific excellence and enjoyable stay in spring-like Espoo and Finland. Kristiina Hilden Anu Koivula Kristiina Kruus Markus Linder Maija Tenkanen 11th Carbohydrate Bioengineering Meeting, 2015, Finland 1 Carbohydrate Bioengineering Meeting History CBM1 1995 Elsinore, Denmark CBM2 1997 La Rochelle, France CBM3 1999 Newcastle, United Kingdom CBM4 2001 Stockholm, Sweden CBM5 2003 Groningen, The Netherlands CBM6 2005 Barcelona, Spain CBM7 2007 Braunschweig, Germany CBM8 2009 Ischia, Naples, Italy CBM9 2011 Lisbon, Portugal CBM10 2013 Prague, Czech Republic 11th Carbohydrate Bioengineering Meeting, 2015, Finland 2 International Programme Committee Birte Svensson (chair) Technical University of Denmark, Denmark Vincent Bulone Royal Institute of Technology, Stockholm, Sweden Pedro Coutinho CNRS, Aix-Marseille Université, France Gideon J. Davies University of York, United Kingdom Lubbert Dijkhuizen University of Groningham, the Netherlands Ten Feizi Imperial College, London, United Kingdom Carlos Fontes Technical University of Lisbon, Portugal Vladimir Kren Academy of Sciences of the Czech Republic, Czech Republic Takashi Kuriki Ezaki Glico Co. Ltd.,Osaka, Japan Marco Moracci CNR, Naples, Italy Carsten Andersen Novozymes A/S, Copenhagen, Denmark Antoni Planas Universitat Ramon Lull, Barcelona, Spain Magali Remaud-Simeon INSA, Toulouse, France Steve G. Withers University of British Columbia, Vancouver, Canada Local Organizing Committee Maija Tenkanen (chair) University of Helsinki, Finland Kristiina Hilden University of Helsinki, Finland Anu Koivula VTT, Finland Kristiina Kruus VTT, Finland Markus Linder Aalto University, Finland 11th Carbohydrate Bioengineering Meeting, 2015, Finland 3 11th Carbohydrate Bioengineering Meeting 10-13 May, 2015 Espoo, Finland Programme Sunday 10.5.2015 16.00 Opening of CBM11 Chair: Birte Svensson 16.05 Welcome speech President Tuula Teeri Aalto University, Finland 16.20 Opening lecture T1: From the first CBHI to biorefineries Merja Penttilä VTT, Finland Glycomics, systems glycobiology and bioinformatics Chair: Markus Linder 17.00 T2: CAZyChip: a bioChip for bacterial glycoside hydrolases detection and dynamic exploration of microbial diversity for plant cell wall hydrolysis Claire Dumon Université de Toulouse, France 17.20 T3: A new generation of chromogenic substrates for high-throughput screening of glycosyl hydrolases, LPMOs and proteases Julia Schückel University of Copenhagen, Denmark 17.40 T4: Mining fungal diversity for novel carbohydrate acting enzymes Ronald P. de Vries Utrecht University, The Netherlands 18.00 End of the day 19 – 21 Get-together, Design Factory 11th Carbohydrate Bioengineering Meeting, 2015, Finland 5 Monday 11.5.2015 Mechanisms of carbohydrate-acting enzymes I Chair: Takashi Kuriki 9.00 Key-note lecture T5: The increasing diversity of lytic polysaccharide monooxygenases Gideon Davies University of York, UK 9.40 T6: Neutron and high-resolution X-ray structural studies of glycoside hydrolase family 45 endoglucanase from the basidiomycete Phanerochaete chrysosporium Kiyohiko Igarashi University of Tokyo, Japan 10.00 T7: New insight into substrate specificity and activity determinants of a starch debranching enzyme gained from substrate: enzyme crystal structures Marie S. Møller Carlsberg Laboratory, Denmark 10.20 T8: Crystal structures of N-acetylhexosamine 1-kinase and UDP-glucose 4-epimerase in the GNB/LNB pathway from infant-gut associated bifidobacteria Shinya Fushinobu University of Tokyo, Japan 10.40 Coffee break and poster viewing Mechanisms of carbohydrate-acting enzymes II Chair: Anu Koivula 11.20 T9: Crystal structure of the GTFB enzyme, the first representative of the 4,6-α-glucanotransferase subfamily within GH70 Tjaard Pijning University of Groningen, The Netherlands 11.40 T10: Catalytic mechanism of retaining glycosyltransferases: Is Arg293 on the β-face of EXTL2 compatible with it? Insights from QM/MM calculations Laura Masgrau Universitat Autònoma de Barcelona, Spain 12.00 T11: Structure-function studies of enzymes in the oxidative D-galacturonate pathway Helena Taberman University of Eastern Finland, Finland 12.20 Flash presentations (5 min each) Chair: Antoni Planas P15: A single point mutation near the active center is responsible for high efficiency of the Thermotoga maritima α-galactosynthase in the synthesis of known amylase substrate Kirill Bobrov B.P.Konstantinov Petersburg Nuclear Physics Institute, Russia 11th Carbohydrate Bioengineering Meeting, 2015, Finland 6 P16: Insights into LPMO diversity from structural and functional characterization of NcLPMO9C, a broad-specificity lytic polysaccharide monooxygenase Anna S. Borisova Swedish University of Agricultural Sciences, Sweden. P32: Assisting effect of a carbohydtrate binding module on glycosynthase- catalyzed polymerization Magda Faijes Universitat Ramon Llull, Spain P43: Rational design of a novel cyclodextrin glucanotransferase from Carboxydocella to improve alkyl glycoside synthesis Kazi Zubaida Gulshan Ara Lund University, Sweden P44: Development and application of a synthetic cellulosome-based screening platform for enhanced enzyme discovery Johnnie Hahm Novozymes, Inc. USA P49: Neopullulanase subfamily and related specificities of the family GH13 - in silico study focused on domain evolution Stefan Janecek Slovak Academy of Sciences, Slovakia P50: Characterization of a GH30 glucuronoxylan specific xylanase from Streptomyces turgidiscabies C56 Satoshi Kaneko University of the Ryukyus, Japan P53: Solution structures of glycosaminoglycans and their complexes with complement Factor H: implications for disease Sanaullah Khan University College London, UK 13.00 Lunch Carbohydrates in health Chair: Vladimir Kren 14.20 Key-note lecture T12: Polysaccharide engineering: towards carbohydrate drugs and drug carriers Takeshi Takaha Ezaki Glico Co., Ltd. Japan 15.00 T13: Structure and mechanism of action of O-acetyltransferase (Oat) A Anthony J. Clarke University of Guelph, Canada 15.20 T14: Complete switch from α2,3- to α2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign Katharina Schmölzer Austrian Centre of Industrial Biotechnology, Austria 11th Carbohydrate Bioengineering Meeting, 2015, Finland 7 15.40 Poster session and coffee