BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS • CELL INVASION ASSAYS •

DIRECTED IN VIVO ANGIOGENESIS ASSAYS • COMETASSAYTM •

TREVIGEN PAR/PARP/PARG • PRODUCT FOCUS and price list 2008-2009MAY 2008

TACSTM IN SITU APOPTOSIS DETECTION KITS •

1.800.TREVIGEN • www.trevigen.com

2008 TREVIGEN PRODUCT FOCUS AND PRICE LIST

Product Overviews

BME Tools for 3D Culture Assays TACS™ Basement Membrane Extract Products and 3D Culture Cell Proliferation Assay (96 well) ...... 10 In Situ Apoptosis Detection Kits ...... 18-23 Specialty Proteins ...... 2-5 3D Culture Cell Harvesting Kit ...... 11 Annexin DIVAA™ PAR/PARP/PARG For monitoring apoptosis via flow cytometry ...24-25 Directed in vivo Angiogenesis Assays ...... 6-7 Kits, Reagents, Antibodies, and Enzymes ...... 12-14 Oxidative Stress Kits Cell Invasion/Migration Assays CometAssay™ ...... 26-27 Kits, Reagents, and Plates ...... 8-9 Kits, Reagents, and Slides ...... 15-17

Trevigen Product International Cell Assays ...... 25 Price List ...... 28-38 Distributors ...... 39-40

Ordering Information • Please use product names and catalog numbers when ordering. Conditions Trevigen products are sold for Research Use Only. • Please provide your name, purchase order number, shipping address, billing address, and telephone number on each order. Warranty • Trevigen accepts VISA, MASTERCARD, and AMERICAN EXPRESS. Trevigen warrants that products will perform according to specifications accompanying each product. Products found not meeting specifications will be replaced, free of charge. Trevigen’s liability is limited to • Fax, phone, e-mail, or mail your order to: replacement of the product. Product discrepancies must be reported Trevigen, Inc. within 5 days of receipt of order. 8405 Helgerman Court Gaithersburg, MD 20877 1-800-873-8443 • Fax: 301-560-4973 Other Local Phone: 301-216-2800 Prices are subject to change. Trevigen reserves the right to change e-mail: [email protected] product specifications. Products may vary somewhat from the catalog description. • Order online at www.trevigen.com

Shipments All shipments are made through our carrier unless otherwise requested. Shipping charges are prepaid and added to your invoice. For RUSH orders, please advise Trevigen’s Customer Service Representative at the time of order.

Trademarks Trademarks: Trevigen, Cultrex, TACS•XL, SNIPase, CultreCoat, and PathClear are registered trademarks, and 3D Culture Matrix, TACS, AngioRack, Apoptosis Grade, BloxALL, Cytonin, Cytonin IHC, DASH, DIVAA, NeuroPore, PeroxyGlow, TumorTACS, FlowTACS, CardioTACS, TiterTACS, TACS-Sapphire, NeuroTACS, DermaTACS, VasoTACS, TACS-Nuclease, TACS Blue Label, DePsipher, MitoShift, CometAssay, CometSlide, FLARE, REC, CellSperse, HC20+, and TreviGel are trademarks of Trevigen, Inc. TACS: Trevigen Apoptotic Cell System ©2008 Trevigen, Inc. All rights reserved.

www.trevigen.com 1 CULTREX® BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS

Basement membranes are continuous sheets underneath. This is achieved by cell-matrix Functions of Basement Membranes of specialized extracellular matrix that are adhesions through cell adhesion molecules • Anchor epithelium to underlying stroma found at the base of all lumen-lining epithelial (CAMs). The basement membrane acts as • Mechanical barrier to cell invasion and endothelial cells. They are composed of a mechanical barrier, preventing malignant • Separates two different tissue types specialized basement membrane proteins, cells from invading the deeper tissues. The • Promotes and maintains cell differentiation including collagen IV (the primary structural basement membrane is also essential for • Filtration element of the basement membrane), angiogenesis. Basement membrane proteins • Storage depot for growth factors laminin I, heparan sulfate proteoglycan, have been found to accelerate differentiation and entactin. The primary function of the of endothelial cells. They also play an basement membrane is to anchor the important role in glomerular filtration in the epithelium to its loose connective tissue kidney.

Basement Membrane Components

Fibrillar collagen matrix (collagen I)

Type VI collagen

Basement membrane

Collagen IV

Laminin Sarcolemma Integrin Growth Factor Receptors

Dystroglycan- Dystrophin Sarcoglycan complex

2 1.800.873.8443 BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS

CULTREX® BASEMENT MEMBRANE EXTRACT PRODUCTS BME PRODUCTS Multipurpose Cultrex® BME (Basement Membrane Extract) Multipurpose Cultrex® BME (Basement Membrane Extract) Description Size Catalog No. ® 5 ml 3430-005-01 Multipurpose BME is available in BME types and our specialty BME matrices Cultrex BME with Phenol Red concentrations ranging from 12-17 mg/ are available in trial sizes for your testing Cultrex® BME with Phenol Red, 5 ml 3431-005-01 Reduced Growth Factor ml and is qualified for angiogenesis, convenience. BME lots can be reserved for Cultrex® BME, No Phenol Red 5 ml 3432-005-01 tumorigenicity assays, migration, growth, your research requirements. Each lot of BME ® differentiation, and neurite outgrowth has passed sterility testing following USP Cultrex BME, No Phenol Red, 5 ml 3433-005-01 Reduced Growth Factor assays. It is available either reduced or XXIV Chapter 71 sterility test and is negative non-reduced in growth factors and either for mycoplasma by PCR. Cultrex® BME PathClear® with phenol red or without. All four standard Description Size Catalog No. Cultrex® BME with Phenol Red, 5 ml 3430-005-02 Cultrex® BME PathClear® PathClear® Cultrex® BME with Phenol Red, 5 ml 3431-005-02 ® BME PathClear is sterile for bacterial and mycoplasma as well as 31 other pathogens Reduced Growth Factor fungal growth according to USP XXIV, Chapter and viruses including LDEV. Patent applied PathClear® 71. It has been tested and cleared for for. Cultrex® BME, No Phenol Red, 5 ml 3432-005-02 PathClear® Cultrex® BME, No Phenol Red, 5 ml 3433-005-02 Cultrex® 3D Culture Matrix™ Reduced Growth Factor PathClear® Trevigen provides three different matrices Laminin I and the 3D Culture Matrix™ capable of forming 3D substrates for cell Collagen I may be used as a gel on which Cultrex® 3D Culture Matrix™ culture and differentiation. The 3D Culture to grow cells or a media additive alone or Description Size Catalog No. Matrix™ BME is our reduced growth factor in concert with other basement membrane Cultrex® 3D BME, No Phenol 15 ml 3445-048-01 BME qualified, lot-to-lot, to promote components to study cellular growth and Red, Reduced Growth Factor differentiation of human epithelial cell lines differentiation in three dimensions in vitro. Cultrex® 3D Laminin I 5 ml 3446-005-01 derived from mammary gland (MCF-10A) 3D Culture Matrix™ Laminin I is available Cultrex® 3D Collagen I - 20 ml 3447-020-01 and human prostate (PC-3) into acinar at a consistent concentration of 6 mg/ml Rat Tail structures. This matrix provides the foundation (by absorbance and extinction co-efficient) ® ® for cells to grow in three dimensions allowing (BCA) and the 3D Culture Matrix™ Collagen Cultrex HC20+™ BME PathClear for the formation of structures in vitro and is I at 5 mg/ml (Sircol Assay). Description Size Catalog No. available at a consistent concentration range Cultrex® High Protein BME 5 ml 3444-005-02 of 12-17 mg/ml. The 3D Culture Matrix™ (HC20+™) PathClear®

SPECIALTY PROTEINS Cultrex® HC20+™ BME PathClear® Description Size Catalog No. ® ® Cultrex HC20+™ BME is available at PathClear has been tested and cleared of Cultrex® Bovine Collagen I 50 mg 3442-050-01 a concentration of 20 mg/ml or greater 31 pathogens and viruses including LDEV Cultrex® Rat Collagen I 100 mg 3440-100-01 for those requiring a high concentration (Lactate Dehydrogenase Elevating Virus) Cultrex® Mouse Collagen IV 1 mg 3410-010-01 BME for in vivo angiogenesis assays making it ideal for in vivo work. Patent ® and tumorigenicity assays. Cultrex® BME pending. Patent applied for. Cultrex Mouse Laminin I 1 mg 3400-010-01 Cultrex® Bovine Fibronectin 1 mg 3416-001-01 ® 50 μg 3417-001-01 CITATIONS Cultrex Bovine Vitronectin Interleukin-6 is a potent growth factor for ER--positive human breast cancer ACCESSORY PRODUCTS A. Kate Sasser, Nicholas J. Sullivan, Adam W. Studebaker, Lindsay F. Hendey, Amy E. Axel, and Brett M. Hall FASEB J, Jun 2007; 21: 3763 - 3770. Description Size Catalog No. Cultrex® Poly-L-Lysine 100 ml 3438-100-01 Identifi cation of proangiogenic TIE2-expressing monocytes (TEMs) in human peripheral blood and cancer Mary Anna Venneri, Michele De Palma, Maurilio Ponzoni, Ferdinando Pucci, Cristina Scielzo, Erika Zonari, Roberta Mazzieri, Claudio Doglioni, and Luigi Naldini Blood, Jun 2007; 109: 5276 – 5285.

www.trevigen.com 3 SPECIALTY PROTEINS BME PRODUCTS Trevigen offers a range of specialty proteins including Mouse Laminin I, Mouse Collagen IV, ® Multipurpose Cultrex BME (Basement Membrane Extract) Rat Collagen I, Bovine Collagen I, Bovine Fibronectin and Bovine Vitronectin. Description Size Catalog No. Cultrex® BME with Phenol Red 5 ml 3430-005-01 Collagen I Cultrex® BME with Phenol Red, 5 ml 3431-005-01 Reduced Growth Factor Collagen I is the major structural component helix scaffold when at a neutral pH and Cultrex® BME, No Phenol Red 5 ml 3432-005-01 of extracellular matrices found in connective 37°C. Collagen I promotes cell attachment, Cultrex® BME, No Phenol Red, 5 ml 3433-005-01 tissue and internal organs. Collagen I is growth, differentiation, migration, and Reduced Growth Factor most prevalent in the dermis, tendons, and tissue morphogenesis during development. bone. It is a 300 kDa molecule composed Trevigen offers Collagen I derived from rat Cultrex® BME PathClear® of two alpha1(I) chains and one alpha2(I) tail and from bovine tendon. Description Size Catalog No. chain that spontaneously forms a triple Cultrex® BME with Phenol Red, 5 ml 3430-005-02 PathClear® CITATIONS Cultrex® BME with Phenol Red, 5 ml 3431-005-02 Suppression of oncogenic properties of c-Myc by LKB1-controlled epithelial organization Reduced Growth Factor PathClear® Johanna I. Partanen, Anni I. Nieminen, Tomi P. Mäkelä, and Juha Klefstrom PNAS, Sep 2007; 104: 14694 – 14699. Cultrex® BME, No Phenol Red, 5 ml 3432-005-02 PathClear® Cultrex® BME, No Phenol Red, 5 ml 3433-005-02 Collagen IV Reduced Growth Factor PathClear® Collagen IV is the primary collagen found comprises up to 10% of the total tumor mass. in the extracellular basement membrane It can be used as a filter, for coating tissue Cultrex® 3D Culture Matrix™ matrices, separating a variety of epithelial culture surfaces, to promote cell attachment Description Size Catalog No. and endothelial cells from the underlying and proliferation and to study its effects on Cultrex® 3D BME, No Phenol 15 ml 3445-048-01 tissues. Trevigen’s mouse collagen IV is cell behavior. It is also used to coat invasion Red, Reduced Growth Factor purified from the EHS sarcoma, where it assay plates. Cultrex® 3D Laminin I 5 ml 3446-005-01 Cultrex® 3D Collagen I - 100 mg 3447-020-01 CITATIONS Rat Tail Retinoschisin Is a Peripheral Membrane Protein with Affi nity for Anionic Phospholipids and Aff ected by Cultrex® HC20+™ BME PathClear® Divalent Cations Camasamudram Vijayasarathy, Yuichiro Takada, Yong Zeng, Ronald A. Bush, and Paul A. Sieving Description Size Catalog No. Invest. Ophthalmol. Vis. Sci., Mar 2007; 48: 991 – 1000. Cultrex® High Protein BME 5 ml 3444-005-02 (HC20+™) PathClear® Laminin I SPECIALTY PROTEINS Laminin is an extracellular matrix protein differentiation, including neurite outgrowth. Description Size Catalog No. which contains a number of functional It is composed of α1β1γ1chain with a total Cultrex® Bovine Collagen I 50 mg 3442-050-01 domains. Trevigen’s highly purified Mr of 800,000 and is used for coating Cultrex® Rat Collagen I 100 mg 3440-100-01 Laminin I, from the EHS sarcoma, increases culture dishes and plates for invasion assays. Cultrex® Mouse Collagen IV 1 mg 3410-010-01 cell adhesion, migration, growth, and At high concentrations the coating will gel. Cultrex® Mouse Laminin I 1 mg 3400-010-01 Cultrex® Bovine Fibronectin 1 mg 3416-001-01 CITATIONS Cultrex® Bovine Vitronectin 50 μg 3417-001-01 Heparanase cleavage of perlecan heparan sulfate modulates FGF10 activity during ex vivo submandibular gland branching morphogenesis Vaishali N. Patel, Sarah M. Knox, Karen M. Likar, Colin A. Lathrop, Rydhwana Hossain, Siavash Eftekhari, ACCESSORY PRODUCTS John M. Whitelock, Michael Elkin, Israel Vlodavsky, and Matthew P. Hoff man Development, Dec 2007; 134: 4177 – 4186. Description Size Catalog No. Cultrex® Poly-L-Lysine 100 ml 3438-100-01

4 1.800.873.8443 BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS

Fibronectin Fibronectin is a 440 kDa, soluble disulfide- both cell-cell and cell-matrix interactions. BME PRODUCTS linked dimer composed of two 220 kDa Fibronectin functions either as a general Multipurpose Cultrex® BME (Basement Membrane Extract) peptide chains. It is an extracellular matrix cell adhesion molecule or as a modulator Description Size Catalog No. protein that is found abundantly in blood, in binding between cell surfaces and the Cultrex® BME with Phenol Red 5 ml 3430-005-01 connective tissues, and provisional matrices extracellular matrix by means of a central ® associated with malignant transformation cell-binding domain, RGD (Arg-Gly-Asp). Cultrex BME with Phenol Red, 5 ml 3431-005-01 Reduced Growth Factor of migratory cells. Fibronectin functions in Cultrex® BME, No Phenol Red 5 ml 3432-005-01 Cultrex® BME, No Phenol Red, 5 ml 3433-005-01 Vitronectin Reduced Growth Factor Vitronectin is an extracellular soluble membrane-damaging effect of the terminal Cultrex® BME PathClear® disulfide-linked dimer composed of a 75 cytolytic complement pathway, and binds to kDa and a 65 kDa peptide chain with a several serpin (serine protease inhibitors). Description Size Catalog No. molecular weight of 140 kDa. Vitronectin is Vitronectin can be used for coating tissue Cultrex® BME with Phenol Red, 5 ml 3430-005-02 PathClear® a major plasma glycoprotein that promotes culture surfaces to promote cell adhesion or ® cellular adhesion and spreading, inhibits the as an additive for serum-free medium. Cultrex BME with Phenol Red, 5 ml 3431-005-02 Reduced Growth Factor PathClear® Cultrex® BME, No Phenol Red, 5 ml 3432-005-02 Accessory Products PathClear® Poly-L-Lysine, a highly positively-charged culture. This solution is provided ready to Cultrex® BME, No Phenol Red, 5 ml 3433-005-02 amino acid chain, is commonly used as a use at 0.01% and contains polymers in the Reduced Growth Factor PathClear® coating agent to promote cell adhesion in 70,000-150,000 kDa range. Cultrex® 3D Culture Matrix™ TESTIMONIALS Description Size Catalog No.

® “We have used Cultrex® in all of our in vitro (endothelial cell tube formation, stem cell diff erentiation), ex vivo (aortic Cultrex 3D BME, No Phenol 15 ml 3445-048-01 Red, Reduced Growth Factor explant outgrowth, salivary gland organ diff erentiation), and in vivo assays (subcutaneous angiogenesis, tumor growth promotion) where it has worked well with highly reproducible fi ndings. Th e fast delivery and availability was highly Cultrex® 3D Laminin I 5 ml 3446-005-01 appreciated by me and my staff .” Cultrex® 3D Collagen I - 100 mg 3447-020-01 Hynda K. Kleinman, guest, NIH Rat Tail

“Th e product is of the highest quality, I’m particularly impressed with the quality assurance program (PathClear® ® ® BME) you have in place. We look forward to continued use of your products.” Cultrex HC20+™ BME PathClear Henry Lopez, President/CSO MuriGenics, Inc. Description Size Catalog No. ® 5 ml 3444-005-02 “After comparing head-to-head Trevigen’s Cultrex® BME and BD Biosciences Matrigel™ BME, Trevigen’s Cultrex® Cultrex High Protein BME (HC20+™) PathClear® equaled or exceeded BD’s Matrigel™ BME product in our 3D Tumor Growth Assay (TGA). Cultrex® BME has a lower retail cost, higher average BME concentration, and superior lot-to-lot consistency with respect to tumor cell growth in the 3D TGA (Sasser, et al. 2007 Cancer Letters). Th ank you for making an excellent product at an aff ord- SPECIALTY PROTEINS able cost.” Brett M. Hall, Ph.D. Description Size Catalog No. Assistant Professor of Pediatrics, Th e Ohio State University School of Medicine Cultrex® Bovine Collagen I 50 mg 3442-050-01 Investigator, Center for Childhood Cancer, Columbus Children’s Research Institute Cultrex® Rat Collagen I 100 mg 3440-100-01 Cultrex® Mouse Collagen IV 1 mg 3410-010-01 Cultrex® Mouse Laminin I 1 mg 3400-010-01 Cultrex® Bovine Fibronectin 1 mg 3416-001-01 Cultrex® Bovine Vitronectin 50 μg 3417-001-01

ACCESSORY PRODUCTS

Description Size Catalog No. Cultrex® Poly-L-Lysine 100 ml 3438-100-01

www.trevigen.com 5 DIRECTED IN VIVO ANGIOGENESIS ASSAYS

DIVAA™ addresses the short-comings of from incorporation in host tissues, ensuring are implanted subcutaneously in the dorsal the plug assay by controlling the site for that there will be samples for analysis. flank of nude mice. Accompanied with the angiogenesis, increasing reproducibility, Whilst size and volume impose a limit of onset of angiogenesis, vascular endothelial conserving test compounds, and supplying one plug per mouse, the compactness of cells proceed to grow into the BME and form more statistical data for quantitative analysis. the angioreactor conserves precious test vessels in the angioreactor. As early as nine When injecting plugs subcutaneously, compounds and permits implantation of up days post-implantation, there are enough researchers have little control over where the to four angioreactors per mouse, allowing cells to determine an effective dose response bolus forms, but with DIVAA™, researchers for multiple data points for quantitative to angiogenic modulating factors. The sleek may standardize the placement of analysis. design of the angioreactor provides a angioreactors and create a stable During the course of the assay, implant standardized platform for reproducible and site for angiogenesis. While plugs form grade silicone cylinders closed at one end, quantifiable in vivo angiogenesis assays. amorphous shapes that are synonymous called angioreactors, are filled with basement These kits include PathClear® BME which has with re-absorption, the angioreactor membrane extract (BME) premixed with or been tested and cleared of a battery of 31 provides a fixed volume and surface area without angiogenic-modulating factors. The pathogens and viruses including LDEV. for reaction. This increases inter- and intra- angioreactors (positive control, negative assay reproducibility, and protects the matrix control, and experimental angioreactors)

Evaluation of Angiogenesis Activation using DIVAA™

8 7

6 5 4 3 2

1 Fluorescence Relative to Control 0 neg CTRL 100 ng FGF 37.5 ng FGF/12.5 ng VEGF Data provided by John Basile

FITC-Lectin detection 9 days post-implantation in nude mice.

6 1.800.873.8443 DIVAA™ DIRECTED IN VIVO ANGIOGENESIS ASSAYS

DIVAA™ Starter Kit The Directed In Vivo Angiogenesis Assay growth factor to induce angiogenesis all 48 DIVAA™Kits (DIVAA™) Starter Kit was designed to angioreactors, and an AngioRack™ designed introduce the technology and give the user to hold the angioreactors during the course Description Size Catalog No. practical experience assessing angiogenesis. of assay setup. DIVAA™ Starter Kit 48 Tests 3450-048-SK It contains 48 angioreactors, enough DIVAA™ Activation Kit 48 Tests 3450-048-K DIVAA™ Inhibition Kit 48 Tests 3450-048-IK AngioRack™ 1 Rack 3450-048-09 DIVAA™ Activation Kit The Directed In Vivo Angiogenesis Assay growth factor for eight positive controls. (DIVAA™) Activation Kit was designed DIVAA™ has been employed in evaluating References for assessing angiogenesis activation. It the enhancement of angiogenesis associated 1. Th e eff ects of adrenomedullin overexpression in contains 48 angioreactors and enough with adrenomedullin1 and CD972. breast tumor cells. Martinez A, Vos M, Guedez L, Kaur G, Chen Z, Garayoa M, Pio R, Moody T, Stetler-Stevenson WG, Kleinman HK, Cuttitta F. DIVAA™ Inhibition Assay J. Natl. Cancer Inst. Aug 2002; 94(16):1226-37. The Directed In Vivo Angiogenesis Assay angioreactors. DIVAA™ has been utilized in 2. CD97, an adhesion receptor on infl ammatory (DIVAA™) Inhibition Kit was designed for demonstrating the inhibition of angiogenesis cells, stimulates angiogenesis through binding assessing angiogenesis inhibition. It contains in matrix metalloprotease (MMP)-2-deficient integrin counterreceptors on endothelial cells. 48 angioreactors and enough growth mice3 and by TIMP-24. Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler- Stevenson WG, Kelly K. factor to induce angiogenesis in all 48 Blood Apr 2005; 105(7):2836-44.

3. Quantitative Assessment of Angiogenic Response DIVAA™ AngioRack™ by the Directed In Vivo Angiogenesis Assay. Guedez L, Rivera AM, Salloum R, Miller ML, Dieg- Available as a separate product, the sterile silicone angioreactors in the laminar mueller JJ, Bungay PM, Stetler- Stevenson WG: autoclavable, reusable AngioRack™ is flow hood while filling and preparing for AJP May 2003; 162(5):1431-1439. precision machined of Teflon® to hold 48 implantation. 4. TIMP-2 Mediated Inhibition of Angiogenesis: An MMP-Independent Mechanism. Seo D, Li H, Guedez L, Wingfi eld PT, Diaz T, Sal- loum R, Wei B, Stetler-Stevenson WG: Cell July 2003; 25:114:171-180.

CITATIONS

Deletion of Yin Yang 1 Protein in Osteosarcoma Defective angiogenesis, endothelial migration, MT1-MMP Controls Tumor-induced Angiogen- Cells on Cell Invasion and CXCR4/Angiogenesis proliferation and MAPK signaling in Rap1b-de- esis through the Release of Semaphorin 4D and Metastasis fi cient mice John R. Basile, Kenn Holmbeck, Th omas H. Bugge, Filomena de Nigris, Raff aele Rossiello, Concetta Magdalena Chrzanowska-Wodnicka, Anna E. and J. Silvio Gutkind Schiano, Claudio Arra, Sharon Williams-Ignarro, Kraus, Daniel Gale, Gilbert C. White, II, and Jillian J. Biol. Chem., Mar 2007; 282: 6899 – 6905. Antonio Barbieri, Alessandro Lanza, Antonio Bales- VanSluys trieri, Maria Teresa Giuliano, Louis J. Ignarro, and Blood, Nov 2007; 10.1182/blood-2007-08-109710. Dissecting the role of endothelial SURVIVIN Ex3 Claudio Napoli in angiogenesis Cancer Res., Mar 2008; 68: 1797 - 1808. Extended-Release Formulations in Epilepsy Hugo Caldas, Jason R. Fangusaro, Daniel R. Boué, Alberto Verrotti, Carmela Salladini, Giovanna Di Michael P. Holloway, and Rachel A. Altura Defective angiogenesis, endothelial migration, Marco, Floriana Pisciella, and Francesco Chiarelli Blood, Feb 2007; 109: 1479 – 1489. proliferation, and MAPK signaling in Rap1b-de- J Child Neurol, Apr 2007; 22: 419 – 426. fi cient mice Magdalena Chrzanowska-Wodnicka, Anna E. Th e Trp Cage Motif as a Scaff old for the Display Kraus, Daniel Gale, Gilbert C. White, II, and Jillian of a Randomized Peptide Library on Bacterio- VanSluys phage T7 Blood, Mar 2008; 111: 2647 - 2656. Richard E. Herman, Douglas Badders, Mark Fuller, Ekaterina G. Makienko, Michael E. Houston, Jr., Matrix Metalloproteinase-activated Anthrax Steven C. Quay, and Paul H. Johnson Lethal Toxin Demonstrates High Potency in J. Biol. Chem., Mar 2007; 282: 9813 – 9824. Targeting Tumor Vasculature Shihui Liu, Hailun Wang, Brooke M. Currie, Al- fredo Molinolo, Howard J. Leung, Mahtab Moayeri, John R. Basile, Randall W. Alfano, J. Silvio Gutkind, Arthur E. Frankel, Th omas H. Bugge, and Stephen H. Leppla J. Biol. Chem., Jan 2008; 283: 529 – 540.

www.trevigen.com 7 Cell Invasion/Migration Assays FOR QUANTITATING THE DEGREE TO WHICH INVASIVE CELLS PENETRATE A BARRIER…

Cultrex® and CultreCoat® Cell Invasion vitro in response to chemoattractants and/ Assays were created in an effort to accelerate or inhibiting compounds. The Cultrex® the screening process for compounds Cell Invasion Assay has been adapted to that influence cell migration through multiple formats so that cell invasion may extracellular matrices. These assays offer be evaluated against different extracellular a flexible, standardized, high throughput matrices; Basement Membrane Extract format for quantitating the degree to which (BME), Laminin I, Collagen I, Collagen IV. invasive cells penetrate a barrier consisting of basement membrane components in

Exploded View Top View Cover

Chamber

Receiver Plate

TESTIMONIALS

“My company has been using Trevigen’s Cultrex® Collagen I and IV Cell Invasion As- “I have used Trevigen’s Cultrex® 96 well Cell Invasion Assay for several months last year says to screen numerous compounds. We have found that these kits are reliable and the and found it is simple, fast and reliable assay. By reading fl uorescent absorbance, it is results are always consistent. Th e dependability of these kits is essential when screening easy to run many samples in a 96-well plate. Th erefore, this assay may potentially be a variety of compounds in a limited amount of time. We had tested a variety of cell used for compound screening.” invasion assay kits on the market and found that nothing compared to the quality of Pharmaceutical Researcher Trevigen’s Cell Invasion Assays.” Vivian Barry, Scientist, Arresto Biosciences “Th e CultreCoat® plates worked out great. I have struggled with the cell invasion assay for a long time. With the CultreCoat® Cell Invasion Assay from your company, I fi nally can close my project. Th e assay is nice and easy and most importantly, the variation between replicates is small and bias is minimal.” Lan Chun Tu Ph. D. Research Scientist, Institute for Systems Biology

8 1.800.873.8443 CELL INVASION/MIGRATION ASSAYS

CultreCoat® Cell Invasion Assay Features • Pre-coated inserts save time. CELL INVASION ASSAYS • Pre-coated inserts improve reproducibility of results. Description Size Catalog No. • Complete kit format. ® • Coating process assures uniform layers of BME plate to CultreCoat 24 Well RGF BME 24 Samples 3460-024-K Cell Invasion Assay plate, and lot to lot, to minimize variability. Cultrex® 24 Well BME Cell 24 Samples 3455-024-K Invasion Assay Cultrex® 24 Well Collagen I 24 Samples 3457-024-K Cultrex® Cell Invasion Assay Features Cell Invasion Assay Cultrex® 24 Well Collagen IV 24 Samples 3458-024-K • Basement Membrane Extract (BME), • Each lot of Cultrex Cell Invasion Cell Invasion Assay Laminin I, Collagen I, and Collagen IV Assay kits has been tested to ensure Cultrex® 24 Well Laminin I 24 Samples 3456-024-K formats. that invasive cell line MDA-MB-231, Cell Invasion Assay • Kit components include matrix, coating penetrates a barrier consisting of an 8 Cultrex® 96 Well BME Cell 96 Samples 3455-096-K solution, invasion chamber, buffers, and micron polyester membrane coated with Invasion Assay Calcein AM. basement membrane components while Cultrex® 96 Well Collagen I 96 Samples 3457-096-K • The assay has been functionally tested the non-invasive cell line MCF7 does not Cell Invasion Assay with MCF7, HT1080, NIH3T3, and penetrate this barrier. Cultrex® 96 Well Collagen IV 96 Samples 3458-096-K Cell Invasion Assay MDA-MB-231 cell lines. • Available in a 96 or 24 well format. Cultrex® 96 Well Laminin I 96 Samples 3456-096-K Cell Invasion Assay Cultrex® Endothelial Cell 96 Samples 3471-096-K 96 Well Cell Invasion Plate Invasion Asay • The receiver plate is compatible with 96 • The chamber is designed so that the well fluorescent readers which eliminates assay may be performed without the requirement for transfer, saving time disassembly until the final analysis. This and reducing error. format helps prevent contamination from • The receiver plate was designed handling and may be adapted for high specifically for fluorescence detection throughput screening (robotic). and its black surface eliminates • The chamber also includes a clear crosstalk between wells and minimizes receiver plate for colorimetric detection. background.

FOR QUANTITATING THE DEGREE TO WHICH CELLS MIGRATE IN RESPONSE TO CHEMOATTRACTANTS...

Cultrex® Cell Migration Assay Features CELL MIGRATION ASSAYS • Quantitative assay to measure the degree • Available in a 96 or 24 well format. Description Size Catalog No. of cell migration in vitro. Cultrex® 24 Well Cell 24 Samples 3465-024-K • Kit components include cell invasion Migration Assay chamber, buffers and Calcein AM. Cultrex® 96 Well Cell 96 Samples 3465-096-K Migration Assay

TREVIGEN PROVIDES A CONTRACT SCREENING SERVICE USING THESE ASSAYS THROUGH TREVIGEN CELL ASSAYS LLC – SEE PAGE 25 FOR DETAILS

www.trevigen.com 9 Tools for 3D Culture Assays

In vitro 3-Dimensional (3D) cell cultures under normal and pathological situations. use of appropriate extracellular matrix cues. produce valuable models that mimic 3D Tissue function depends on the interactions Trevigen’s portfolio of 3D culture products tissue organization and function and enhance between cells and the extracellular matrix; now includes five new kits for 3D Culture the understanding of cell/tissue function thus, effective 3D cell cultures rely on the assays.

3D CULTURE CELL PROLIFERATION ASSAY (96 WELL) ...FOR INVESTIGATING CELL PROLIFERATION IN 3D CULTURE

Recent studies indicate that the composition translation to animal models. The Cultrex® a flexible, standardized, high-throughput of the extracellular environment influences 3D Culture Cell Proliferation Assays provide format for quantitating the degree to which the cellular response to apoptosis inducing a more physiologically relevant assessment pharmacological compounds influence agents implicating a role for extracellular when using cell models in the screening toxicity or tumorigenicity, and in an in vivo- proteins in influencing both toxicity and drug process for compounds that influence like environment. resistance. As a result, this environment toxicity, cell survival, tumorigenicity, tumor must be mimicked during the course of cell- formation. These assays are available with based studies to provide the most accurate BME, Laminin I or Collagen I and offer

Features 3D Culture BME Cell Proliferation Assay Components • First standardized kits engineered for assessing the effects of Component Size Component No. compounds or genetic alteration on cell proliferation in 3D Culture 3D BME 5 ml 3445-001-01 • Available with Cultrex® Basement Membrane Extract (BME), Cell Proliferation Reagent 3 ml 3445-096-02 Laminin I, or Collagen I • High Throughput 96 stripwell format 3D Culture 96 Stripwell Plates 2 Plates 3445-096-03 • One step colorimetric detection • Quantitative Analysis • This assay can determine EC50 Values (effective concentration) of between 1000 -100,000 cells per well.

80000

70000 The Cultrex® 3D Culture Cell Proliferation Assay is compatible with both Top and Embedded 3D 60000 Culture models. For Top Cultures, 96 stripwells were coated with 35 μl of BME, and cells 50000 were seeded at 5,000 cells per well in 200 μl medium containing 2% BME. For Embedded 40000 cultures, 96 stripwells were coated with 30 μl of BME, and cells were suspended in cold BME. 30000 Next, 15 μl of BME containing 5,000 cells was

Cells per Well added to each well, and allowed to gel for 30 20000 minutes before 200 μl of assay medium was added to each well. Cells were maintained in 10000 3D culture for 5 days before quantitating cell numbers using the Cultrex® 3D Culture Cell 0 Proliferation Assay. MCF-10A MCF-7 MCF-10A MCF-7

Top Embedded

10 1.800.873.8443 TOOLS FOR 3D CULTURE ASSAYS

3D CULTURE CELL HARVESTING KIT ...FOR HARVESTING CELLS FROM 3D CULTURE FOR BIOCHEMICAL ANALYSIS

3D cultures exhibit cellular behaviors and preparations. Non-enzymatic methodologies Ordering Information morphologies similar to those seen in vivo, have also been described for depolymerizing Description Size Catalog No. however, the adaptation of these models for extracellular matrix proteins, although the 3D Culture Cell 96 Well 3445-096-CK studying biochemical processes has been implementation of these protocols remains Proliferation Assay Core Kit impeded by the challenge of separating intact problematic for some researchers. Trevigen’s 3D Culture BME Cell 96 Well 3445-096-K cells from extracellular proteins comprising Cultrex® 3D Culture Cell Harvesting Kit Proliferation Assay the substrate or matrix. Commonly, provides an optimized and standardized 3D Culture Laminin I Cell 96 Well 3446-096-K proteases are employed to degrade these solution for the isolation and normalization Proliferation Assay extracellular proteins, however, proteases of cell lysates from 3D Culture MatrixTM BME 3D Culture Collagen I Cell 96 Well 3447-096-K also degrade proteins on the cell surface and or Laminin I for subsequent biochemical Proliferation Assay protease activity may carry over into lysate analysis. 3D Culture Cell Harvesting Kit 20 Samples 3448-020-K

Features Cultrex® 3D Culture Matrix™™ • First standardized kit for isolating cells • Ideal for Western Blotting Component Size Component No. from 3D culture for biochemical analysis • May also be used for RNA and DNA Cultrex® 3D Culture Matrix BME 1 ml 3445-001-01 • Non-enzymatic formulation preserves analysis Cultrex® 3D Culture Matrix BME 5 ml 3445-005-01 extracellular receptors and prevents ® 15 ml 3445-048-01 subsequent degradation during 3D Harvesting Kit Components Cultrex 3D Culture Matrix BME processing. Cultrex® 3D Culture Matrix 100 mg 3447-020-01 Component Size Catalog No. Collagen I • Overcomes the issue of extracting cells Anti-h/m G3PDH 20 μl 2275-PC-020 ® 5 ml 3446-005-01 from BME or Laminin I in 3D models Cultrex 3D Culture Matrix 10X Cell Harvesting Buffer 100 ml 3448-020-01 Laminin I – an improvement on existing cell harvesting techniques 10X Cell Wash Buffer 100 ml 3448-020-02 • Includes buffers for cell lysates and a Sample Buffer 10 ml 3448-020-03 G3PDH antibody for normalizing loading 10X Loading Buffer 1 ml 3448-020-04

2D 3D BME

Morphology of MCF-10A (A, B) and MCF-10A MCF-7 cells (C, D) in traditional 2D culture and 3D BME culture, scale = 250 μm.

MCF-7

TESTIMONIALS

“Th e Trevigen BME extraction kit exceeded my expectations- it was able to dissolve both Matrigel and Cultrex BME effi ciently, without enzymatic digestion, and with little residual matrix polymer remaining after the procedure. I was able to scale the protocol to accommodate diff erent size culture applications with only minor modifi cations. As the kit includes not only protein extraction solutions compatible with the common BCA protein determination assay, but anti-GAPDH antibody for use as a loading control in Western immunob- lots, I would highly recommend the Trevigen BME extraction kit over dipase, collagenase and other non-enzymatic extraction methods.” Jason Fritz, Research Scientist

www.trevigen.com 11 PARP/PAR/PARG KITS, ENZYMES, AND ANTIBODIES

When activated by DNA strand breaks, death during ischemia and other conditions could prevent the removal of PAR from PARP-1 uses NAD as a substrate to form that generate extensive DNA damage. PARG PARP-1. Because PARP-1 is inhibited by ADP-ribose polymers on itself and on specific may maintain the active state of PARP-1 by extensive poly-(ADP-ribosyl)ation, PARG acceptor proteins such as histones, DNA continuously removing inhibitory ADPribose inhibitors could thereby indirectly inhibit polymerases, DNA ligases, p53, and Fos. residues from PARP-1. The regulation of PARP-1 activity. Prior work has shown that These polymers are in turn rapidly degraded PARG activity may therefore influence PARP- the PARG inhibitor gallotannin can markedly by PARG, a ubiquitously expressed exo- and mediated cell death. First, PARG inhibition reduce death of astrocytes after oxidative endoglycohydrolase. Excessive activation of could slow the turnover of PAR and thereby stress. PARP-1 leads to NAD depletion and cell limit NAD depletion. Second, PARG inhibition

Nicotinamide O

NH2

N ADPR NAD+ O NH2 Nuclear Protein N NH2 N + N O O N N O P O P O O O OH OH PARP-1, PARP-2 OH OH HO OH

Energy PARG Metabolism DNA STRAND NADH BREAKS NH Free ADPR 2 N N O O N ADP/ATP N Nuclear O Proteins O P O P O O OH OH OH OH HO OH

In response to genotoxic stress Poly (ADP-ribose) polymerase-1(PARP-1) binds to single strand breaks in DNA and synthesizes highly negatively charged and branched poly (ADP-ribose) (PAR) polymers, from NAD+, that covalently modify numerous nuclear proteins including PARP-1 These highly branched polymers are in turn rapidly degraded by poly (ADP-ribose) glycohydrolase (PARG). As a consequence of PARP activation, extensive DNA damage can lead to the depletion of NAD+ and in turn reduce the capacity of the cell to generate energy in the form of ATP resulting in cell death.

12 1.800.873.8443 PARP/PAR/PARG KITS AND REAGENTS

Standard and High Specific Activity PARP Enzyme PARP/PAR/PARG KITS, ENZYMES AND Trevigen offers a “standard” PARP purified to ~50% purity and a high specific activity ANTIBODIES (HSA) enzyme purified to greater than 95%. Standard and High Specific Activity PARP Enzyme The standard PARP enzyme is useful as a Description Size Catalog No. positive control for Western blot analysis of Recombinant Human PARP 50 μl 4667-50-01 ribosylated proteins and PARP Activity Assays. Enzyme HSA PARP is recommended for detailed Recombinant Human PARP 250 μl 4667-250-01 studies and drug discovery applications. Enzyme Recombinant Human PARP 1 ml 4667-1K-01 Enzyme High-Specific Activity Recom- 1,000 Units 4668-100-01 PARP Catalytic Domains binant Human PARP Enzyme High-Specific Activity Recom- 5,000 Units 4668-500-01 PARP Catalytic Domains are recombinant binant Human PARP Enzyme proteins purified from E. coli containing High-Specific Activity Recom- 20,000 Units 4668-2K-01 plasmids harboring the PARP-CAT gene. binant Human PARP Enzyme

PARP Catalylic Domains Description Size Catalog No. PARP Catalytic Domain 100 μg 4669-100-02 (Human) PARP Catalytic Domain (Chicken) 100 μg 4669-100-01

PARP and PAR Antibodies Description Size Catalog No. PARP and PARG Kits PARP MAb (Clone C2-10) 50 μg 4338-MC-50 The PARP Assay Kits measure incorporation PAR MAb (Clone 10HA) 100 μl 4335-MC-100 of biotinylated Poly (ADPribose) onto histone PAR MAb (Clone 10HA) 1000 μl 4335-MC-1K proteins in 96-well plates. The assays allow PAR PAb (Rabbit) 100 μl 4336-BPC-100 screening of PARP inhibitors and measurement Poly (ADP) Ribose (PAR) Polymer 100 μl 4336-100-01 of PARP activity in cell and tissue extracts. PAR Affinity Purified MAb 50 μl 4335-AMC-050 PARG Assay Kits allow screening of PARG (Clone 10HA) inhibitors and measurement of PARG activity PAR Affinity Purified PAb 50 μl 4336-APC-050 in cell extracts. PARP and PARG Kits Description Size Catalog No. PARP Assay Kit (2) (Co) 96 Well 4671-096-K TESTIMONIALS PARP Assay Kit (2) (Ch) 96 Well 4675-096-K

“Monoclonal antibody against poly (ADP- ribose) polymerase (PARP) worked really well in my experiments and I HT PARP Assay Kit (1) (Ch) 96 Well 4676-096-K would recommend using it to everyone for detecting total PARP.” HT PARP Assay Kit (1) (Co) 96 Well 4677-096-K Aleksey Kazantsev, PhD, Harvard Medical School HT PARP Apoptosis Assay Kit 96 Well 4684-096-K (1) (Co) “Trevigen Colorimetric PARP Assay Kit is user friendly. A non-radioactive method, to quantify the (ADP) activity in cell lines.” HT PARP Apoptosis Assay Kit 96 Well 4685-096-K Dr. Senthilkumar B. Rajamohan, University of Chicago, Dept of Cardiothoracic Surgery (1) (Ch) HT F Homogenous PARP 96 Tests 4690-096-K “Monoclonal antibody against poly (ADP- ribose) polymerase (PARP) worked really well in my experiments and I Inhibition Assay would recommend using it to everyone for detecting total PARP.” PARG Assay Kit (1) (Ch) 96 Well 4682-096-K Dr. Peyman Kabolizadeh, Virginia Commonwealth University PARG Assay Kit (1) (Co) 96 Well 4683-096-K 96 Stripwell Histone-coated 1 Plate 4677-096-P Plate –natural 96 Stripwell Histone-coated 1 Plate 4678-096-P Plate –white TREVIGEN PROVIDES A CONTRACT SCREENING SERVICE USING THESE ASSAYS (1) With Histone Coated Strip Wells, (2) With Histone Reagent THROUGH TREVIGEN CELL ASSAYS LLC – SEE PAGE 25 FOR DETAILS (Co) Colorimetric detection, (Ch) Chemiluminescent detection (F) Fluorescent detection www.trevigen.com 13 PARP and PAR Antibodies

PARP/PAR/PARG KITS, ENZYMES AND The PARP monoclonal antibody can be ANTIBODIES used for analysis of PARP protein levels Standard and High Specific Activity PARP Enzyme and cleavage of PARP in apoptotic cells. It Description Size Catalog No. recognizes both the full length protein (116 kDa) and an 85 kDa cleavage fragment Recombinant Human PARP 50 μl 4667-50-01 Enzyme associated with apoptosis.The PAR antibody Recombinant Human PARP 250 μl 4667-250-01 is specific for PAR polymers synthesized by Enzyme a variety of PARP isoforms (PARP-1,-2,-3, Recombinant Human PARP 1 ml 4667-1K-01 tankyrase, vPARP, and others). Enzyme High-Specific Activity Recom- 1,000 Units 4668-100-01 binant Human PARP Enzyme High-Specific Activity Recom- 5,000 Units 4668-500-01 binant Human PARP Enzyme TREVIGEN PARP Assay Kits High-Specific Activity Recom- 20,000 Units 4668-2K-01 Universal PARP Assay Kit Universal PARP Assay Kit PARP Apoptosis Kit Homogenous binant Human PARP Enzyme (Histone Coated Stripwells) (Coat your own – with histone PARP Assay reagent and stripwells) Kit PARP Catalylic Domains Description Size Catalog No. PARP Catalytic Domain 100 μg 4669-100-02 (Human) Colorimetric Detection Chemiluminescence Detection Colorimetric Detection Chemiluminescence Detection Colorimetric Detection Chemiluminescence Detection Fluorescence Detection PARP Catalytic Domain (Chicken) 100 μg 4669-100-01 Catalog No. 4671-096-K 4675-096-K 4675-096-K 4676-096-K 4684-096-K 4685-096-K 4690-096-K PARP and PAR Antibodies Assay Type NAD-Biotin NAD-Biotin NAD-Biotin NAD-Biotin PAR-ELISA PAR-ELISA NAD Consumption Description Size Catalog No. Sample Type Purifi ed Purifi ed Purifi ed Purifi ed lysates lysates Purifi ed PARP MAb (Clone C2-10) 50 μg 4338-MC-50 PARP PARP PARP PARP PARP PAR MAb (Clone 10HA) 100 μl 4335-MC-100 Sensitivity 0.01 Units 0.01 Units 0.01 Units 0.01 Units 500 cells/well 500 cells/well 10% inhibition PAR MAb (Clone 10HA) 1000 μl 4335-MC-1K PARP/well PARP/well PARP/well PARP/well Inhibitor Inhibitor Inhibitor Inhibitor Apoptosis Apoptosis HT Inhibitor PAR PAb (Rabbit) 100 μl 4336-BPC-100 Applications Screen Screen Screen Screen Screen Poly (ADP) Ribose (PAR) Polymer 100 μl 4336-100-01 Amount 1 Unit 1 Unit 1 Unit 1 Unit 2000 cells or 2000 cells or 1 μl inhibitor PAR Affinity Purified MAb 50 μl 4335-AMC-050 Assayed PARP/well PARP/well PARP/well PARP/well 20 ng lysates 20 ng lysates 200 ng PARP (clone 10HA) PAR Affinity Purified PAb 50 μl 4336-APC-050 Prep Time 3 hr 3 hr 21 hr 21 hr 1 hr 1 hr .5 hr Assay Time 2 hr 2 hr 1 hr 1 hr 8 hr 8 hr 1 hr PARP and PARG Kits Elapsed Time 5 hr 5 hr 22 hr 22 hr 9 hr 9 hr 1.5 hr Description Size Catalog No. PARP Assay Kit (2) (Co) 96 Well 4671-096-K PARP Assay Kit (2) (Ch) 96 Well 4675-096-K Ask about our new PAR ELISA HT PARP Assay Kit (1) (Ch) 96 Well 4676-096-K HT PARP Assay Kit (1) (Co) 96 Well 4677-096-K HT PARP Apoptosis Assay Kit 96 Well 4684-096-K CITATIONS (1) (Co) Direct phosphorylation and regulation of poly (ADP-ribose) polymerase-1 by extracellular signal-regu- HT PARP Apoptosis Assay Kit 96 Well 4685-096-K (1) (Ch) lated kinases 1/2 Tiina M. Kauppinen, Wai Y. Chan, Sang Won Suh, Amanda K. Wiggins, Eric J. Huang, and Raymond A. HT F Homogenous PARP 96 Tests 4690-096-K Swanson. Inhibition Assay PNAS, May 2006; 103: 7136 - 7141. PARG Assay Kit (1) (Ch) 96 Well 4682-096-K Minocycline inhibits poly (ADP-ribose) polymerase-1 at nanomolar concentrations PARG Assay Kit (1) (Co) 96 Well 4683-096-K Conrad C. Alano, Tiina M. Kauppinen, Andreu Viader Valls, and Raymond A. Swanson 96 Stripwell Histone-coated 1 Plate 4677-096-P PNAS, Jun 2006; 103: 9685 - 9690. Plate –natural 96 Stripwell Histone-coated 1 Plate 4678-096-P Apoptotic Splenocytes Drive the Autoimmune Response to Poly (ADP-ribose) Polymerase 1 in a Murine Plate –white Model of Lupus Th omas Grader-Beck, Livia Casciola-Rosen, Th omas J. Lang, Roman Puliaev, Antony Rosen, and Charles S. Via (1) With Histone Coated Strip Wells, (2) With Histone Reagent J. Immunol., Jan 2007; 178: 95 - 102. (Co) Colorimetric detection, (Ch) Chemiluminescent detection (F) Fluorescent detection 14 1.800.873.8443 CometAssay™ KITS REAGENTS AND SLIDES

CometAssay™ KITS REAGENTS AND SLIDES

The ability of chemical substances, isolated time consuming, resource intensive and from samples of outdoor and indoor require a proliferating cell population. For the airborne particulates, to induce mutagenicity Hence newer and more sensitive test systems in prokaryotic cells and eukaryotic cells have now been introduced for assessing the is well studied. These chemicals have genotoxicity of chemicals. The single cell gel been found to interact with the vital tissue electrophoresis or CometAssay™ is one such direct macromolecules regulating the cellular state-of-the-art technique for quantitating functions leading to long lasting health DNA damage and repair from in vivo and disorders. Acute and chronic exposure to in vitro samples of eukaryotic cells and several of these environmental chemicals some prokaryotic cells. This technique is measurement such as pesticide, metals, polycyclic aromatic rapid, non-invasive, sensitive, visual, and hydrocarbons (PAHs), solvents etc. have inexpensive compared to conventional been shown to produce marked toxicity at techniques and is a powerful tool to of the target sites. Some of these chemicals study factors modifying mutagenicity and affect the DNA, which is the carrier of carcinogenicity. It is the only technique that inherited information and any gross change directly measures DNA damage in individual in its structure potentiates serious biological cells and as a result has rapidly gained DNA Damage changes. Hence there is a need to test importance in the fields of genetic toxicology the chemicals for their genotoxic potential and human biomonitoring. CometAssay™ before being released into the environment. measures double strand breaks (DSBs), The conventional methods for evaluating single strand breaks (SSBs), alkalilabile sites, genetic damage include chromosomal oxidative DNA base damage, DNA-DNA/ aberration, micronucleus assay and sister DNA-protein/DNA-Drug crosslinking, and chromatid exchanges. However, these are DNA repair.

Cells mixed with low melting Treat cells with Lysis Solution (removes membranes Immobilize cells on CometSlide™ 1 agarose at 37°C (LM Agarose) 2 3 and histones from the DNA)

Samples treated with alkali Samples stained with intercalating dye and Alkaline electrophoresis performed (reveals DNA breaks) 4 (unwinds and denatures DNA) 5 6 visualized by epifluorescence microscopy

www.trevigen.com 15 CometAssay™ Kits CometAssay™ KITS REAGENTS AND SLIDES Trevigen’s CometAssay™provides reagents is able to migrate out of the cell during and our exclusive CometSlide™ for the electrophoresis and can be visualized CometAssay™ Kits rapid analysis of DNA fragmentation using SYBR® Green I nucleic acid gel stain. Description Size Catalog No. associated with DNA damage. Following Cells that have accumulated DNA damage CometAssay™ Kit 50 Samples 4250-050-K alkaline lysis, the unwound, relaxed DNA appear as fluorescent comets with tails of CometAssay™ Silver Kit 50 Samples 4251-050-K DNA fragmentation or unwinding, whereas, normal undamaged DNA does not migrate CometAssay™ 40 Samples 4252-040-K HT Sample Kit far from the origin. The CometAssay™ is CometAssay™ 96 Well Kit 96 Samples 4253-096-K provided with our exclusive CometSlides™ which greatly simplify the assay. Simply add CometAssay™ Components your cells to the low melting point Comet Description Size Catalog No. LMAgarose, and pipet onto the slide. CometAssay™ Lysis Solution 2 x 500 ml 4250-050-01 CometAssay™ LMAgarose 15 ml 4250-050-02 200 mM EDTA; pH 10 12.5 ml 4250-050-04

CometSlides™ and FLARE™ Slides TESTIMONIALS Description Size Catalog No. CometSlide™ (2 well slide) 25 Slides 4250-050-03 “I initially purchased the CometAssayTM Control Cells for the purpose of training others to manually score Comet cells. Th e slides were straightforward to prepare, and were an excellent addition to the training regime used for my CometSlide™ (2 well slide) 100 Slides 4250-200-03 colleagues. Not only are the 4 diff erent types of control cells signifi cantly diff erent when comparing % tail DNA, they CometAssay™ HT Slide 25 Slides 4252-500-01 are also easily diff erentiated under the scope or with image analysis. Th is was an added benefi t for training. In the (20 well slide) future I will use the CometAssayTM Control cells as additional controls in our in vitro/in vivo Comet studies to ensure CometAssay™ HT Slide 100 Slides 4252-02K-01 study reproducibility between trials and individual experimenters.” (20 well slide) Larry Gladnick, Pharmaceutical Scientist 96 Well CometSlide™ 10 Slides 4253-960-03 “I have been using the Trevigen CometAssay™ reagents and slides for several years and have found the products to be (96 well slide) excellent in terms of quality, reproducibility and pricing. In addition the products come with an impressive customer 96 Well CometSlide™ 20 Slides 4253-02K-03 support service and excellent technical competence. Trevigen has been quick to respond to our technical needs and (96 well slide) have been critical to our success in developing new markers for risk assessment.” 96 Well CometSlide™ 100 Slides 4253-10K-03 Randa El-Zein, MD, PhD, Th e University of Texas, M.D. Anderson Cancer Center (96 well slide) FLARE™ Slides 25 Slides 3950-075-02 (3 well slide) FLARE™ Assays and Slides FLARE™ Slides 100 Slides 3950-300-02 Trevigen’s unique FLARE™ (Fragment Length Each slide provides surfaces specially treated (3 well slide) Analysis using Repair Enzymes) Assays provide to promote agarose adherance, and a CometSlide™ Rack System each 4252-040-02 the ability to detect DNA damage in single hydrophobic barrier to allow treatment with cells using a variety of DNA repair enzymes one of Trevigen’s DNA repair enzymes. FLARE™ Assay Kits in conjunction with Trevigen’s CometAssay™ Description Size Catalog No. single gel electrophoresis kit. To assess the writing surface Fpg FLARE™ Kit 75 Samples 4040-100-FK type of DNA Damage induced by putative Endonuclease III FLARE™ Kit 75 Samples 4045-01K-FK mutagen, drug, or treatment regimen, T4-PDG FLARE Kit 75 Samples 4055-100-FK cells are harvested after treatment and immobilized in a layer of low melting point specially treated cv-PDG FLARE™ Kit 75 Samples 4065-100-FK glass surface agarose on the FLARE™ slide. Trevigen’s UVDE FLARE™ Kit 75 Samples 4100-100-FK three well FLARE™ Slide can be interchanged Human OGG1 FLARE™ Kit 75 Samples 4130-100-FK with the CometSlide™. FLARE™ slides allow direct application of LMAgarose without hydrophobic CometAssay™ Control Cells base layers or pretreatment for 3 samples. barrier Description Size Catalog No. CometAssay™ Control 1 Set of 4 groups 4256-010-CC Cells (10 Assays) CITATIONS

Th e CC Chemokine Ligand 2 (CCL2) Mediates Fibroblast Survival through IL-6 ™ Xiangde Liu, Anuk M. Das, Jonathan Seideman, Don Griswold, Chantal N. Afuh, Tetsu Kobayashi, Shinji Ask about our new CometAssay Abe, Qiuhong Fang, Mitsu Hashimoto, Huijung Kim, Xingqi Wang, Lei Shen, Shin Kawasaki, and Stephen I. Electrophoresis System Rennard Am. J. Respir. Cell Mol. Biol., Jul 2007; 37: 121 – 128.

16 1.800.873.8443 CometAssay™ KITS REAGENTS AND SLIDES

CometAssay™ Control Cells: For the standardization of Comet assays CometAssay™ KITS REAGENTS AND SLIDES Trevigen’s CometAssay™ Control Cells are a conditions to increase the amount of CometAssay™ Kits set of cell preparations containing different damage in the three different populations levels of DNA damage to be used with - CC1, -CC2, and -CC3, respectively. These Description Size Catalog No. Trevigen’s CometAssay™ Kits. In a typical cryopreserved control cells are designed to CometAssay™ Kit 50 Samples 4250-050-K comet assay, electrophoresis methods and act as controls to standardize and compare CometAssay™ Silver Kit 50 Samples 4251-050-K differences in cell preparations create a alkaline electrophoresis methods between CometAssay™ 40 Samples 4252-040-K significant source of variation in comet individual users and laboratories. HT Sample Kit tail parameters. Such variation sometimes CometAssay™ 96 Well Kit 96 Samples 4253-096-K makes it difficult to compare results between Components laboratories, and even within the same lab. To overcome this problem, Trevigen Description Size Catalog No. scientists developed a set of stable control CometAssay™ Lysis Solution 2 x 500 ml 4250-050-01 cell populations containing incremental levels CometAssay™ LMAgarose 15 ml 4250-050-02 of DNA damage for use when performing 200 mM EDTA; pH 10 12.5 ml 4250-050-04 the CometAssay™. These control cells, when electrophoresed in the CometAssay™, FLARE™ Assays and Slides consistently produce four distinct populations. Description Size Catalog No. The healthy control cell population (CC0) CometSlide™ (2 well slide) 25 Slides 4250-050-03 was treated with Etoposide under various CometSlide™ (2 well slide) 100 Slides 4250-200-03 CometAssay™ HT Slide 25 Slides 4252-500-01 TESTIMONIALS (20 well slide) CometAssay™ HT Slide 100 Slides 4252-02K-01 “We perform CometAssay™ routinely at the Human Health and Nutrition Research, Kentucky State University, and (20 well slide) are interested in investigating DNA protective capacity of bioactive compounds found in vegetables and fruits. We 10 Slides 4253-960-03 have been using various Trevigen products including the Fpg FLARE™ kit. We are pleased with their performance 96 Well CometSlide™ (96 well slide) quality and services.” Avinash M. Tope, PhD, Principal Investigator, Kentucky State University 96 Well CometSlide™ 20 Slides 4253-02K-03 (96 well slide) 96 Well CometSlide™ 100 Slides 4253-10K-03 (96 well slide) FLARE™ Slides 25 Slides 3950-075-02 CITATIONS (3 well slide) FLARE™ Slides 100 Slides 3950-300-02 Th e CC Chemokine Ligand 2 (CCL2) Mediates Fibroblast Survival through IL-6 (3 well slide) Xiangde Liu, Anuk M. Das, Jonathan Seideman, Don Griswold, Chantal N. Afuh, Tetsu Kobayashi, Shinji Abe,Qiuhong Fang, Mitsu Hashimoto, Huijung Kim, Xingqi Wang, Lei Shen, Shin Kawasaki, and Stephen I. CometSlide™ Rack System Each 4252-040-02 Rennard. Am. J. Respir. Cell Mol. Biol., Jul 2007; 37: 121 – 128. FLARE™ Kits Description Size Catalog No. Regulation of Bcl-xL Expression in Lung Vascular Smooth Muscle Yuichiro J. Suzuki, Hiroko Nagase, Chi Ming Wong, Shilpashree Vinod Kumar, Vivek Jain, Ah-Mee Park, and Fpg FLARE™ Kit 75 Samples 4040-100-FK Regina M. Day. Endonuclease III FLARE™ Kit 75 Samples 4045-01K-FK Am. J. Respir. Cell Mol. Biol., Jun 2007; 36: 678 – 687. T4-PDG FLARE Kit 75 Samples 4055-100-FK cv-PDG FLARE™ Kit 75 Samples 4065-100-FK UVDE FLARE™ Kit 75 Samples 4100-100-FK Human OGG1 FLARE™ Kit 75 Samples 4130-100-FK

CometAssay™ Control Cells Description Size Catalog No. CometAssay™ 1 Set of 4 groups 4256-010-CC Control Cells (10 Assays)

TREVIGEN PROVIDES A CONTRACT SCREENING SERVICE USING THESE ASSAYS THROUGH TREVIGEN CELL ASSAYS LLC – SEE PAGE 25 FOR DETAILS

www.trevigen.com 17 TACS™ IN SITU APOPTOSIS DETECTION KITS

Apoptosis or programmed cell death is a mechanism of cellular suicide that occurs after sufficient cellular damage. Apoptosis results in the condensation of the nucleus, and the cell shrinks. Chromosomal fragmentation due to the controlled digestion of DNA is visible in apoptotic cells. Cytoplasmic blebbing and apoptotic bodies are also Mild convolution seen during apoptosis. The end result of apoptosis is cell death without Chromatin compaction inflammation of the surrounding tissue. and segregation Condensation of cytoplasm Phosphatidyl serine flipping

Blebbing Nuclear fragmentation Cell fragmentation

Phagocytosis

Apoptotic body

Phagocytic cell

18 1.800.873.8443 TACS™ IN SITU APOPTOSIS DETECTION KITS

TISSUE SPECIFIC TACS™ KITS FOR IN SITU APOPTOSIS DETECTION TISSUE SPECIFIC TACS™ KITS FOR IN SITU APOPTOSIS DETECTION Trevigen’s Tissue Specific TACS™ Kits were developed to provide researchers with an effective method for identifying apoptotic cells in specific tissue types. These complete kits provide all the Description Specificity Size Catalog No. reagents required for labeling including permeabilization reagents, labeling and stop buffers, CardioTACS™ Cardiac 30 4827-30-K labeling and detection reagents, TACS-Nuclease™ reagent, and cations. The kits also include in situ Apoptosis Tissue Samples Detection Kit detailed protocols with hints and tips for optimal labeling of samples. DermaTACS™ Kit Dermal 30 4829-30-K in situ Apoptosis Tissue Samples CardioTACS™ Kit Detection Kit NeuroTACS™ II Neural 30 4823-30-K The CardioTACS™ Kit is optimized for in situ Apoptosis Tissue Samples identification of apoptotic cells in cardiac Detection Kit samples. Because the high cellularity of TumorTACS™ Kit Tumor 30 4815-30-K cardiac tissue presents problems in in situ Apoptosis Tissue Samples permeabilization, the kit comes with two Detection Kit permeabilization reagents to provide options. VasoTACS™ Kit Vascular 30 4826-30-K in situ Apoptosis Tissue Samples The kit is based on DNA end-labeling Detection Kit using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue ALL PURPOSE TISSUE IN SITU TACS™ KITS ® Label™ Detection system. Apoptotic rat cardiac myocyte labeled using the TACS•XL CardioTACS™ kit. Rat heart tissue was fixed in 4% Description Stain/Counterstain Size Catalog No. paraformaldehyde overnight followed by paraffin ® embedding. Five micron sections were prepared and TACS•XL TACS Blue Label™ 30 4828-30-BK placed onto glass microscope slides. The samples were Blue Label / Nuclear Fast Red Samples processed following the Cardio-TACS™ kit protocol. Kit Photo courtesy of Dr. J. Zhang, FDA. TACS•XL® DAB / 30 4828-30-DK DAB Kit Methyl Green Samples TACS•XL® 30 4828-30-R CITATIONS Replenisher Samples Kit Preemptive heme oxygenase-1 gene delivery reveals reduced mortality and preservation of left ventricular function 1 yr after acute myocardial infarction TACS™ 2 TdT Xiaoli Liu, Jeremy A. Simpson, Keith R. Brunt, Christopher A. Ward, Sean R. R. Hall, Robert T. Kinobe, Description Stain/Counterstain Size Catalog No. Valerie Barrette, M. Yat Tse, Stephen C. Pang, Alok S. Pachori, Victor J. Dzau, Kofo O. Ogunyankin, and Luis TACS™ 2 TdT- TACS Blue 30 4811-30-K G. Melo Blue Label Kit Label™ Samples Am J Physiol., Heart Circ Physiol., Jul 2007; 293: H48 - H59. TACS™ 2 TdT- DAB 30 4810-30-K DAB Kit Samples TACS™ 2 TdT- Fluorescein 30 4812-30-K Fluorescein Kit Samples TACS™ 2 TdT 30 4810-30-R Replenisher Kit Samples

SPECIALTY IN SITU TACS™ KITS

Description Size Catalog No. FlowTACS™ in situ 60 Samples 4817-60-K Apoptosis Detection Kit TiterTACS™ in situ 1 Plate 4822-96-K Apoptosis Detection Kit (96 Wells)

TACS: Trevigen Apoptotic Cell System

www.trevigen.com 19 DermaTACS™ Kit

TISSUE SPECIFIC TACS™ KITS FOR IN SITU The DermaTACS™ Kit is optimized for protocol with tips for optimal labeling of skin APOPTOSIS DETECTION measuring apoptosis in skin samples. The samples. If you wish to use a preprepared kit is based on DNA end-labeling with TdT positive control you may use either the Cell Description Specificity Size Catalog No. and modified nucleotides. Detection of Culture Control Slides (Cat# 4800-30-20) or CardioTACS™ Cardiac 30 4827-30-K incorporated molecules is achieved using a the Tissue Control Slides (Cat# 4800-30-40). in situ Apoptosis Tissue Samples Detection Kit chromogenic substrate with a horseradish These controls allow you to run through the DermaTACS™ Kit Dermal 30 4829-30-K peroxidase detection system. The extracellular procedure to become familiar with handling in situ Apoptosis Tissue Samples scaffolding in skin tissue can make it problematic the samples, etc. Each set of Control Slides is Detection Kit to permeabilize and high background is a shipped with a product information sheet that NeuroTACS™ II Neural 30 4823-30-K common problem. The DermaTACS™ Kit provides information on the recommended in situ Apoptosis Tissue Samples Detection Kit includes two permeabilization reagent options permeabilization method, incubation times, for permeabilization along with a detailed and interpretation of data. TumorTACS™ Kit Tumor 30 4815-30-K in situ Apoptosis Tissue Samples Detection Kit VasoTACS™ Kit Vascular 30 4826-30-K in situ Apoptosis Tissue Samples Detection Kit

ALL PURPOSE TISSUE IN SITU TACS™ KITS TACS•XL® Description Stain/Counterstain Size Catalog No. Detection of DNA fragmentation in UVB irradiated human skin model, EpiDerm™ with DermaTACS™. Samples were TACS•XL® TACS Blue Label™ 30 4828-30-BK provided courtesy of Dr. Patrick Hayden, MatTek Corporation, Ashland, MA. Blue Label / Nuclear Fast Red Samples Kit CITATIONS TACS•XL® DAB / 30 4828-30-DK DAB Kit Methyl Green Samples Use of Topical Insulin to Normalize Corneal Epithelial Healing in Diabetes Mellitus TACS•XL® 30 4828-30-R Ian S. Zagon, Matthew S. Klocek, Joseph W. Sassani, and Patricia J. McLaughlin Replenisher Samples Arch Ophthalmol, Aug 2007; 125: 1082 - 1088. Kit

TACS™ 2 TdT Description Stain/Counterstain Size Catalog No. NeuroTACS™ II Kit TACS™ 2 TdT- TACS Blue 30 4811-30-K The NeuroTACS™ II Kit is optimized to Label™ Samples Blue Label Kit provide rapid and convenient identification TACS™ 2 TdT- DAB 30 4810-30-K of apoptosis in brain tissue or neuronal cells. DAB Kit Samples The kit has been developed to overcome Fluorescein 30 4812-30-K TACS™ 2 TdT- the common difficulties unique to neuronal Fluorescein Kit Samples samples, including the fragile nature of brain TACS™ 2 TdT 30 4810-30-R Replenisher Kit Samples tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform duel labeling SPECIALTY IN SITU TACS™ KITS experiments to detect cell specific antigens in conjunction with apoptotic cells. Double labeling of mouse brain section for apoptosis Description Size Catalog No. using NeuroTACS™ II (brown) and a monoclonal FlowTACS™ in situ 60 Samples 4817-60-K antibody to GFAP (red) (not included in kit). Brain sections were fixed in 10% neutral buffered formalin, embedded Apoptosis Detection Kit in paraffin and sectioned at 5 microns. The section was TiterTACS™ in situ 1 Plate 4822-96-K counterstained using Trevigen’s Blue Counterstain. Apoptosis Detection Kit (96 Wells) CITATIONS

Caspase 3Dependent Cell Death of Neurons Contributes to the Pathogenesis of West Nile Virus Encepha- litis Melanie A. Samuel, John D. Morrey, and Michael S. Diamond J. Virol., Mar 2007; 81: 2614 - 2623.

TACS: Trevigen Apoptotic Cell System 20 1.800.873.8443 TACS™ IN SITU APOPTOSIS DETECTION KITS

TumorTACS™ Kit The TumorTACS™ Kit provides rapid and TISSUE SPECIFIC TACS™ KITS FOR IN SITU convenient identification of apoptosis in APOPTOSIS DETECTION tumors or cancer calls. The kit has a unique TUNEL-based system that preferentially Description Specificity Size Catalog No. labels the double-stranded DNA found in CardioTACS™ Cardiac 30 4827-30-K apoptotic cells. The kit contains all the in situ Apoptosis Tissue Samples Detection Kit reagents required for permeabilizing, DermaTACS™ Kit Dermal 30 4829-30-K labeling, staining, and counterstaining tumor in situ Apoptosis Tissue Samples samples, including TACS-Nuclease™ for Detection Kit preparing positive controls. NeuroTACS™ II Neural 30 4823-30-K Apoptotic cells within mouse mammary tumor identified in situ Apoptosis Tissue Samples using the TumorTACS™ kit. Mammary tumor was fixed Detection Kit in 4% paraformaldehyde overnight followed by paraffin embedding. Five micron sections were prepared and TumorTACS™ Kit Tumor 30 4815-30-K placed onto glass microscope slides. The sample was in situ Apoptosis Tissue Samples processed following the TumorTACS™ kit protocol. Detection Kit VasoTACS™ Kit Vascular 30 4826-30-K CITATIONS in situ Apoptosis Tissue Samples Detection Kit Cathepsin E Prevents Tumor Growth and Metastasis by Catalyzing the Proteolytic Release of Soluble TRAIL from Tumor Cell Surface Tomoyo Kawakubo, Kuniaki Okamoto, Jun-ichi Iwata, Masashi Shin, Yoshiko Okamoto, Atsushi Yasukochi, ALL PURPOSE TISSUE IN SITU TACS™ KITS Keiichi I. Nakayama, Tomoko Kadowaki, Takayuki Tsukuba, and Kenji Yamamoto TACS•XL® Cancer Res., Nov 2007; 67: 10869 - 10878. Description Stain/Counterstain Size Catalog No. TACS•XL® TACS Blue Label™ 30 4828-30-BK Blue Label / Nuclear Fast Red Samples VasoTACS™ Kit Kit TACS•XL® DAB / 30 4828-30-DK The VasoTACS™ Kit is an effective method DAB Kit Methyl Green Samples for identifying apoptotic cells in vascular TACS•XL® 30 4828-30-R samples, by reducing background and Replenisher Samples Kit improving labeling in vascular tissue. Similar to the CardioTACS™ Kit, it is based on TACS™ 2 TdT DNA end-labeling with TdT and a modified Description Stain/Counterstain Size Catalog No. nucleotide detected using our TACS Blue TACS™ 2 TdT- TACS Blue 30 4811-30-K Label™ detection system. Blue Label Kit Label™ Samples Drug induced apoptosis in the small artery of a rat TACS™ 2 TdT- DAB 30 4810-30-K exhibiting spontaneous hypertension using the Trevigen DAB Kit Samples VasoTACS™ Kit. The tissue was formalin-fixed and paraffin-embedded. Photo courtesy of Dr. Jun Zhang, TACS™ 2 TdT- Fluorescein 30 4812-30-K FDA. Fluorescein Kit Samples TACS™ 2 TdT 30 4810-30-R CITATIONS Replenisher Kit Samples

Attenuated herpes simplex virus 1 blocks arterial apoptosis and intimal hyperplasia induced by balloon angioplasty and reduced blood fl ow SPECIALTY IN SITU TACS™ KITS Christopher L. Skelly, Amito Chandiwal, James E. Vosicky, Ralph R. Weichselbaum, and Bernard Roizman PNAS, Jul 2007; 104: 12474 - 12478. Description Size Catalog No. FlowTACS™ in situ 60 Samples 4817-60-K Apoptosis Detection Kit TiterTACS™ in situ 1 Plate 4822-96-K Apoptosis Detection Kit (96 Wells)

TACS: Trevigen Apoptotic Cell System www.trevigen.com 21 ALL PURPOSE TACS™ KITS TISSUE SPECIFIC TACS™ KITS FOR IN SITU APOPTOSIS DETECTION TACS•XL® In Situ Apoptosis Detection Kits

® Description Specificity Size Catalog No. TACS•XL embodies a new approach for labeling and stop buffers, labeling and CardioTACS™ Cardiac 30 4827-30-K the in situ detection of apoptosis. The detection reagents, and TACS-Nuclease™ in situ Apoptosis Tissue Samples TACS•XL® kit is based on incorporation of reagents for generating positive controls with Detection Kit bromodeoxyuridine (BrdU) at the 3’ OH ends your own samples. DermaTACS™ Kit Dermal 30 4829-30-K of the DNA fragments that are formed during in situ Apoptosis Tissue Samples Detection Kit apoptosis. The incorporation of BrdUTP by TdT is more efficient than either biotinylated NeuroTACS™ II Neural 30 4823-30-K in situ Apoptosis Tissue Samples or digoxigenin labeled nucleotides used in Detection Kit other TUNEL-based assays. The detection TumorTACS™ Kit Tumor 30 4815-30-K system utilizes a biotin conjugated anti- in situ Apoptosis Tissue Samples BrdU antibody and streptavidin-horseradish Detection Kit peroxidase. The combination of antibody VasoTACS™ Kit Vascular 30 4826-30-K in situ Apoptosis Tissue Samples specificity with the signal enhancing properties Detection Kit of biotin:streptavidin results in precise cellular labeling and the highest signal to noise ratio Detection of apoptotic cells in the photoreceptor layer of observed in competitive testing. These kits retina in 10-day-old FVB mice using the TACS•XL® DAB ALL PURPOSE TISSUE IN SITU TACS™ KITS provide all the reagents required for labeling kit. The tissue was fixed in 10% formalin and 5 μM paraffin TACS•XL® sections were used for the assay (400X magnification). including two permeabilization reagents, Photo courtesy Dr. S. Alikunju, Department of Cell Description Stain/Counterstain Size Catalog No. Biology, Baylor College of Medicine, Houston, TX. TACS•XL® TACS Blue Label™ 30 4828-30-BK Blue Label / Nuclear Fast Red Samples Kit TACS™ 2 TdT In Situ Apoptosis Detection Kits TACS•XL® DAB / 30 4828-30-DK DAB Kit Methyl Green Samples The TACS™ 2 TdT Kits utilize Trevigen’s reagents required for labeling including two unique cation optimization system to permeabilization reagents, labeling and stop TACS•XL® 30 4828-30-R Replenisher Samples enhance labeling within particular tissues. buffers, labeling and detection reagents, and Kit A highly purified form of the TdT enzyme TACS-Nuclease™ reagents for generating TACS™ 2 TdT is included in the kits for the enzymatic positive controls with your own samples. incorporation of biotinylated nucleotides. Description Stain/Counterstain Size Catalog No. Biotin labeling is achieved using streptavidin- TACS™ 2 TdT- TACS Blue 30 4811-30-K Blue Label Kit Label™ Samples horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB) or TACS TACS™ 2 TdT- DAB 30 4810-30-K DAB Kit Samples Blue Label™. For fluorescent detection, TACS™ 2 TdT- Fluorescein 30 4812-30-K a fluorescein conjugate of streptavidin is Fluorescein Kit Samples used and visualized by epifluorescence TACS™ 2 TdT 30 4810-30-R microscopy. These kits provide all the Replenisher Kit Samples

SPECIALTY IN SITU TACS™ KITS Detection of apoptosis in post-weaning mouse breast tissue using the TACS™ 2 TdT DAB kit. Tissue sections Description Size Catalog No. were collected and fixed in 10% neutral buffered formalin, embedded in paraffin and sectioned at 5 μM. FlowTACS™ in situ 60 Samples 4817-60-K Deparaffinized and rehydrated sections were processed Apoptosis Detection Kit following the TACS™ 2 protocol. TiterTACS™ in situ 1 Plate 4822-96-K Apoptosis Detection Kit (96 Wells) CITATIONS

Caspase-3 Gene Deletin Prolongs Survival in Polycystic Kidney Disease Yunxia Tao, Iram Zafar, Jun Kim, Robert W. Schrier, and Charles L. Edelstein J. Am. Soc. Nephrol., Apr 2008; 19:749-755.

TACS: Trevigen Apoptotic Cell System 22 1.800.873.8443 TACS™ IN SITU APOPTOSIS DETECTION KITS

SPECIALTY IN SITU TACS™ KITS TISSUE SPECIFIC TACS™ KITS FOR IN SITU FlowTACS™ Kit APOPTOSIS DETECTION

FlowTACS™ takes advantage of Trevigen’s flow cytometry, direct visualization using a Description Specificity Size Catalog No. exclusive in situ labeling technology to label fluorescent microscope or for quantitation CardioTACS™ Cardiac 30 4827-30-K cell samples for processing by flow cytometry. using a fluorometer. in situ Apoptosis Tissue Samples FlowTACS™ also provides flexibility in Detection Kit selection of fluorophores that are compatible DermaTACS™ Kit Dermal 30 4829-30-K in situ Apoptosis Tissue Samples with your research design. The kit is supplied Detection Kit with streptavidin-FITC and allows multi-color NeuroTACS™ II Neural 30 4823-30-K labeling in conjunction with experiment in situ Apoptosis Tissue Samples specific antibodies. This complete kit provides Detection Kit all the reagents required for labeling including TumorTACS™ Kit Tumor 30 4815-30-K two permeabilization reagents, labeling and in situ Apoptosis Tissue Samples Detection Kit stop buffers, labeling and detection reagents, AnalysisAnalysis of murine thymocytesthymocytes at 16 hours after treatment with10 μg/ml cycloheximide (A) and 1 μM dexamethasone VasoTACS™ Kit Vascular 30 4826-30-K and TACS-Nuclease for generating positive (B). Cells were harvested and labeled according to the in situ Apoptosis Tissue Samples controls with your own samples. The kit can FlowTACS™ protocol prior to analysis by flow cytometry. Detection Kit be used for the detection of apoptosis using Data courtesy N. Hardegen, NIH, NIDR, Bethesda, MD. ALL PURPOSE TISSUE IN SITU TACS™ KITS CITATIONS TACS•XL® Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 Description Stain/Counterstain Size Catalog No. receptor signaling TACS•XL® TACS Blue Label™ 30 4828-30-BK Linlin Wang, Th omas C. Schulz, Eric S. Sherrer, Derek S. Dauphin, Soojung Shin, Angelique M. Nelson, Blue Label / Nuclear Fast Red Samples Carol B. Ware, Mei Zahn, Chao-Zhong Song, Xiaoji Chen, Sandii N. Brimble, Amanda McLean, Maria J. Kit Galeano, Elizabeth W. Uhl, Kevin A. D’Amour, Johnathon D. Chesnut, Mahendra S. Rao, Anthony Blau, and Allan J. Robins TACS•XL® DAB / 30 4828-30-DK Methyl Green Samples Blood, Dec 2007; 110: 4111 - 4119. DAB Kit TACS•XL® 30 4828-30-R Replenisher Samples TiterTACS™ Kit Kit The HT TiterTACS™ Colorimetric Apoptosis also provides TACS-Nuclease™ solution to TACS™ 2 TdT Detection Kit takes advantage of Trevigen’s generate positive controls from your own Description Stain/Counterstain Size Catalog No. exclusive in situ labeling technology bringing samples giving you a maximal value for the TACS™ 2 TdT- TACS Blue 30 4811-30-K it to the 96 well microplate format for assay. Blue Label Kit Label™ Samples high throughput quantitative detection of TACS™ 2 TdT- DAB 30 4810-30-K Samples apoptosis in cultured cells. Detection using DAB Kit TACS-Sapphire™, a non-toxic colorimetric TACS™ 2 TdT- Fluorescein 30 4812-30-K Fluorescein Kit Samples substrate, allows both kinetic and endpoint TACS™ 2 TdT 30 4810-30-R readings. The reaction generates a blue Replenisher Kit Samples product that can be measured at 630nm, and will turn yellow after the reaction is stopped with acid allowing endpoint SPECIALTY IN SITU TACS™ KITS reading at 450nm. The HT TiterTACS™Kit Description Size Catalog No. Detection of apoptosis in ML-1 cells after treatment with FlowTACS™ in situ 60 Samples 4817-60-K 1 μM staurosporine. All control wells contained 1x105 cells. Cells were harvested, fixed and labeled according Apoptosis Detection Kit to the TiterTACS™ protocol prior to colorimetric analysis. TiterTACS™ in situ 1 Plate 4822-96-K Reaction was stopped with 2N HCl. Apoptosis Detection Kit (96 Wells)

CITATIONS

Eff ects of gamma radiation on FcRI and TLR-mediated mast cell activation Benjamin P. Soule, Jared M. Brown, Nataliya M. Kushnir-Sukhov, Nicole L. Simone, James B. Mitchell, and Dean D. Metcalfe J. Immunol., Sep 2007; 179: 3276 - 3286.

TACS: Trevigen Apoptotic Cell System www.trevigen.com 23 Annexin FOR MONITORING APOPTOSIS VIA FLOW CYTOMETRY

Apoptosis is a programmed cell death observed on the inner surface facing the to negatively charged phospholipids like process characterized by morphological and cytosol. Cells undergoing apoptosis break up PS in the presence of Ca2+ and shows biochemical features occurring at different the phospholipid asymmetry of their plasma minimal binding to phosphatidylcholine and stages. Once triggered, apoptosis proceeds membrane and expose phosphatidylserine, sphingomyeline. Changes in PS asymmetry, with different kinetics depending on cell which is translocated to the outer layer of which is analyzed by measuring Annexin V types and culminates with cell disruption the membrane. This occurs in the early binding to the cell membrane, were detected and formation of apoptotic bodies. Cell phases of apoptotic cell death during before morphological changes associated surface changes of the dying cells results which the cell membrane remains intact. with apoptosis have occurred and before in the recognition and the uptake of these The flip of phosphatidyl serine from the membrane integrity has been lost. cells by phagocytes. Phospholipids are inner leaflet to the outer leaflet of the cell asymmmetrically distributed between inner membrane represents a hallmark (early and and outer leaflets of the plasma membrane widespread) in detecting dying cells. The with phosphatidylcholine and sphingomyelin anticoagulant properties of Annexin V have exposed on the external leaflet of the lipid proven to be a useful tool in detecting bilayer, and phosphatidylserine predominantly apoptotic cells since it preferentially binds

Ca2+ Ca2+ Ca2+ Ca2+

2+ Outer Cell Membrane Ca

Apoptosis

Inner Cell Membrane

Phosphatidylserine Other Phospholipids Annexin V

24 1.800.873.8443 ANNEXIN and TREVIGEN CELL ASSAYS

TESTIMONIALS ANNEXIN KITS “We have routinely used the Trevigen Annexin V FITC kit for around seven years in order to study the time course and mechanism of cell death in a number cell lines treated with a DNA methylating agent (MMS) combined with Description Size Catalog No. a PARP inhibitor. Use of Annexin V staining, in conjunction with propidium iodide (both provided with the kit), TACS™ Annexin V FITC 100 Samples 4830-01-K has allowed us to quickly and easily distinguish apoptotic from necrotic cells. Th e protocol included in the kit is easy to follow; however for adherent human fi broblasts used here, we have found it necessary to harvest the cells by brief TACS™ Annexin V FITC 250 Samples 4830-250-K trypsinization using 0.05% tiypsin-EDTA, then combine these cells with those already released into the medium. We TACS™ Annexin V Biotin 100 Samples 4835-01-K highly recommend this kit for any lab that is interested in studying apoptosis by fl ow cytometry.” Donna Stefanick, Biologist, Dr. Samuel Wilson’s Lab Laboratory of Structural Biology, National Institute of TACS™ Annexin V Biotin 250 Samples 4835-250-K Environmental Health Sciences Kit Contents “I have been using the Trevigen Annexin V-FITC apoptosis detection kit and found the product to be excellent in • Annexin V FITC or Annexin V Biotin terms of quality, sensitivity and reproducibility. In addition this kit was fast, precise and simple to perform. Trevigen • Binding Buffer provides a responsive customer support service and excellent technical competence. I highly recommend this product.” • Propidium Iodide Mike Fisher, Laboratory Research Specialist, Pharmacology Department University of North Carolina

CITATIONS

Cationic gradient reversal and cytoskeletal independent volume regulatory pathways defi ne an early stage of apoptosis Carl D. Bortner, Maria I. Sifre, and John A. Cidlowski J. Biol. Chem., Mar 2008; 283: 7219-7229.

MAPK and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption Divyendu Singh, Kelly L. McCann, and Farhad Imani Am J Physiol Lung Cell Mol Physiol, Aug 2007; 293: L436 - L445.

Eff ects on neurite outgrowth and cell survival of a secreted fi broblast growth factor binding protein upregulated during spinal cord injury Elena Tassi, Sharon Walter, Achim Aigner, Rafael H. Cabal-Manzano, Ranjan Ray, Paul J. Reier, and Anton Wellstein Am J Physiol Regulatory Integrative Comp Physiol, Aug 2007; 293: R775 - R783.

Selective Regulation of Bone Cell Apoptosis by Translational Isoforms of the Glucocorticoid Receptor Nick Z. Lu, Jennifer B. Collins, Sherry F. Grissom, and John A. Cidlowski Mol. Cell. Biol., Oct 2007; 27: 7143 - 7160.

vFLIP from KSHV inhibits anoikis of primary endothelial cells Sofi a Efklidou, Ranbir Bailey, Nigel Field, Mahdad Noursadeghi, and Mary K. Collins J. Cell Sci., Feb 2008; 121: 450 – 457.

Trevigen Cell Assays LET OUR SCIENTIFIC EXPERTISE WORK FOR YOU

Trevigen Inc., a pioneer in the fields of DNA damage and repair, apoptosis, and cancer cell behavior, offers contract screening services through Trevigen Cell Assays, LLC. Trevigen Cell Assays provides an assay development and sample testing service for Comet, PARP, PARG, cell invasion, cell migration and in vitro angiogenesis assays. As the sole commercial provider of standardized tools for DNA damage testing we are positioned to increase your throughput while maintaining your cost of testing.

CONTACT TREVIGEN, INC. FOR DETAILS AT 800-873-8443 OR [email protected].

www.trevigen.com 25 Oxidative Stress Kits

Oxidative stress is caused by an imbalance through a constant input of metabolic energy. 8-oxo-dG in urine, serum and saliva between the production of reactive oxygen Disturbances in this normal redox state can samples as well as activity kits to assist in and a biological system’s ability to readily cause toxic effects through the production of measuring the activity of several compounds detoxify the reactive intermediates or easily peroxides and free radicals that damage all related to oxidative stress, including Cyclic repair the resulting damage. All forms of life components of the cell, including proteins, AMP, Glutathione, Glutathione Reductase, maintain a reducing environment within their lipids, and DNA. Glutathione Peroxidase, and Superoxide cells. This reducing environment is preserved Trevigen’s oxidative stress and product Dismutase. by enzymes that maintain the reduced state portfolio includes an ELISA kit to measure

TYPICAL 8-OXO-DG STANDARD CURVE

3.0

2.5

2.0

1.5

1.0

0.5

0.0 -0.5 0.0 0.5 1.0 1.5 2.0

Log 8-OHdG Concentration (ng/mL)

26 1.800.873.8443 OXIDATIVE STRESS KITS

ELISA KITS OXIDATIVE STRESS KITS 8-hydroxy-2’-deoxyguanosine (8-oxo-dG) fibrosis, atopic dermatitis and rheumatoid is a modified nucleoside base, which is arthritis. Parkinson’s disease, Alzheimer’s Description Size Catalog No. the most commonly studied and detected disease and Huntington’s disease are 8-oxo-dG ELISA 1 Plate (96 wells) 4370-096-K by-product of DNA damage that is excreted neurodegenerative diseases that are thought in the urine upon DNA repair. Urinary 8-oxo- to be caused by exposure to neurotoxins dG and its analogs, 8-hydroxyguanosine in people with a genetic predisposition for ACTIVITY KITS and 8-hydroxyguanine, are linked to many these diseases. Oxidative stress is associated Description Size Catalog No. degenerative diseases. The association of with the pathogenesis of these diseases and HT Glutathione Kit 4 Plates (384 wells) 7511-100-K reactive oxygen species (ROS) and the use of elevated levels of DNA damage have been HT Glutathione 5 Plates (480 wells) 7513-500-K 8-oxo-dG as a biomarker of oxidative stress measured in a wide range of neurological Reductase Kit have been investigated in many diseases, conditions. HT Glutathione 5 Plates (480 wells) 7512-100-K including bladder and prostate cancer, cystic Peroxidase Kit HT SOD Kit 5 Plates (480 wells) 7501-500-K CITATIONS HT Cyclic AMP 1 Plate (96 wells) 2500-096-K HT Direct Cyclic AMP 1 Plate (96 wells) 2501-096-K Maternal diet infl uences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats J. L. Tarry-Adkins, M. S. Martin-Gronert, J. -H. Chen, R. L. Cripps, and S. E. Ozanne FASEB J, June 2008; 22: 2037-2044.

CITATIONS

Interplay between cellular methyl metabolism and adaptive effl ux during oncogenic transformation from chronic arsenic exposure in human cells Jean-François Coppin, Wei Qu, and Michael P. Waalkes J. Biol. Chem., May 2008; 10.1074/jbc.M802942200.

TESTIMONIALS

“Th e Trevigen HT Glutathione Peroxidase Assay Kit was used with a spectrophotometer to quantify the GP present in the tissues of tomato plants treated with platinum group metals (PGMs). Th e kit was used as the main means for determining and quantifying the amount of oxidative damage in the tomato plants as a result of these PGM treat- ments. Th e product was extremely helpful to our research team in assessing these eff ects and the company also provided great customer service and support. Trevigen has contributed greatly to our educational experiences in research and aided us to learn more about our specifi c scientifi c interests.” Erica Zubalake, Researcher, Marist College Poughkeepsie, NY

www.trevigen.com 27 2008 PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. 8-oxo-dG ELISA Kit DNA Damage • Human • ELISA 96 WELLS 4370-096-K Oxidative Stress 8-oxo-dG Monoclonal Antibody, DNA Damage • Multiple • ELISA 50 UL 4354-MC-50 clone 2E2 Oxidative Stress • Immunocytochemistry Aag Enzyme DNA Damage • Multiple • Comet Assay™/FLARE™ 100 UNITS 4090-100-EB Oxidative Stress • DNA nicking • Supercoiled relaxation assays • DNA fragmentation analysis Aag Enzyme DNA Damage • Multiple • Comet Assay™/FLARE™ 500 UNITS 4090-500-EB Oxidative Stress • DNA nicking • Supercoiled relaxation assays • DNA fragmentation analysis Cultrex® Tube Formation Angiogen- Cell Behavior • Mammalian • Detection of inducers and inhibitors of 96 WELLS 3470-096-K esis Detection Kit endothelial cell tube formation DIVAA™ Angiogenesis Activation Kit Cell Behavior • Mammalian • Quantitative in vivo angiogenesis analysis 48 TESTS 3450-048-K DIVAA™ Angiogenesis Inhibition Kit Cell Behavior • Mammalian • Quantitative in vivo angiogenesis analysis 48 TESTS 3450-048-IK DIVAA™ Angiogenesis Starter Kit Cell Behavior • Mammalian • Quantitative in vivo angiogenesis analysis 48 TESTS 3450-048-SK DIVAA™ Angiorack Cell Behavior Not Applicable • Angioreactor loading 1 RACK 3450-048-09 Annexin V Biotin Kit Apoptosis • Multiple • Apoptosis detection (fl ow cytometry) 100 TESTS 4835-01-K Annexin V Biotin Kit Apoptosis • Multiple • Apoptosis detection (fl ow cytometry) 100 TESTS 4835-250-K Annexin V Biotin Protein Apoptosis • Multiple • Fluorescent labeling (apoptotic cells) 100 UL 4835-01-01 Annexin V Biotin Protein Apoptosis • Multiple • Fluorescent labeling (apoptotic cells) 250 UL 4835-250-1 Annexin V FITC Kit Apoptosis • Multiple • Apoptosis Detection (fl ow cytometry) 100 TESTS 4830-01-K Annexin V FITC Kit Apoptosis • Multiple • Apoptosis Detection (fl ow cytometry) 100 TESTS 4830-250-K Annexin V FITC Protein Apoptosis • Multiple • Fluorescent labeling (apoptotic cells) 100 UL 4830-01-01 Annexin V FITC Protein Apoptosis • Multiple • Fluorescent labeling (apoptotic cells) 250 UL 4830-250-01 AP Endonuclease Enzyme DNA Damage • Multiple • DNA nicking 1000 UNITS 4110-01K-EB Oxidative Stress AP Endonuclease Enzyme DNA Damage • Multiple • DNA nicking 5000 UNITS 4110-05K-EB Oxidative Stress APAF-1 Monoclonal Antibody, Apoptosis • Human • Western blot 50 UL 2340-MC-050 clone 94408.11 TACS™ 2 TDT Apoptosis Detection Apoptosis • Multiple • Apoptosis detection (tissue sections) 30 SAMPLES 4811-30-K Kit TACS™ 2 TDT Fluorescein Apoptosis Apoptosis • Multiple • Apoptosis detection (tissue sections) 30 SAMPLES 4812-30-K Detection Kit TACS•XL® Blue Label Apoptosis Apoptosis • Multiple • Apoptosis detection (tissue sections) 30 SAMPLES 4828-30-BK Detection Kit TACS•XL® DAB Apoptosis Detection Apoptosis • Multiple • Apoptosis detection (tissue sections) 30 SAMPLES 4828-30-DK Kit TACS•XL® Basic Apoptosis Apoptosis • Multiple • Apoptosis detection (tissue sections) 30 SAMPLES 4828-30-K Detection Kit CardioTACSTM Apoptosis Detection Apoptosis • Multiple • Apoptosis detection 30 SAMPLES 4827-30-K Kit (cardiac tissue sections) DermaTACS™ Apoptosis Detection Apoptosis • Multiple • Apoptosis detection (dermal tissue sections) 30 SAMPLES 4829-30-K Kit FlowTACS™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection (fl ow cytometry) 60 SAMPLES 4817-60-K MitoShift™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection (fl ow cytometry) 100 TESTS 6305-100-K NeuroTACS™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection 30 TESTS 4823-30-K (neuronal tissue sections) TiterTACS™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection (96-well plate) 96 SAMPLES 4822-96-K TumorTACS™ Apoptosis Detection Apoptosis • Multiple • Apoptosis detection (tumor tissue sections) 30 SAMPLES 4815-30-K Kit VasoTACS™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection 30 SAMPLES 4826-30-K (vascular tissue sections) 28 1.800.873.8443 2008 TREVIGEN PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. DePsipher™ Apoptosis Detection Kit Apoptosis • Multiple • Apoptosis detection (fl ow cytometry) 100 SAMPLES 6300-100-K TACS™ DNA Laddering Apoptosis Apoptosis • Multiple • DNA fragmentation 20 SAMPLES 4855-20-K Detection Kit (Chemiluminescent) • Apoptosis detection TACS™ DNA Laddering Apoptosis Apoptosis • Multiple • DNA fragmentation 20 SAMPLES 4857-20-K Detection Kit (Colorimetric) • Apoptosis detection TACS™ DNA Laddering Apoptosis Apoptosis • Multiple • DNA fragmentation 20 SAMPLES 4850-20-ET Detection Kit (Ethidium Bromide) • Apoptosis detection TACS™ DNA Laddering Apoptosis Apoptosis • Multiple • DNA fragmentation 20 SAMPLES 4850-20-K Detection Kit (Isotopic) • Apoptosis detection Bax Beta Polyclonal Antibody Apoptosis • Human • Western blot 250 UL 2283-PC-250 • Immunoprecipitation Bax Monoclonal Antibody, Apoptosis • Mouse • Western blot 100 UL 2280-MC-100 clone YTH-5B7 • Immunoprecipitation • Immunohistochemistry Bax Monoclonal Antibody, Apoptosis • Mouse • Western blot 25 UL 2280-MC-025 clone YTH-5B7 • Immunoprecipitation • Immunohistochemistry Bax Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 2281-MC-100 clone YTH-6A7 • Mouse • Immunoprecipitation • Rat • Immunohistochemistry Bax Monoclonal Antibody, Apoptosis • Human • Western blot 25 UL 2281-MC-025 clone YTH-6A7 • Mouse • Immunoprecipitation • Rat • Immunohistochemistry Bax Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 2282-MC-100 clone YTH-2D2 • Immunoprecipitation • Immunohistochemistry Bax Monoclonal Antibody, Apoptosis • Human • Western blot 25 UL 2282-MC-025 clone YTH-2D2 • Immunoprecipitation • Immunohistochemistry Bcl-2 Family Monoclonal Antibody Apoptosis • Mouse • Western blot 3 VIALS 2325-MC-025 Panel, clone YTH5B7/YTH-10C4/ • Immunoprecipitation YTH-2H12 • Immunohistochemistry Bcl-2 Family Monoclonal Antibody Apoptosis • Human • Western blot 3 VIALS 2330-MC-025 Panel, clone YTH2D2/YTH8C8/ • Immunoprecipitation YTH-2H12 • Immunohistochemistry Bcl-2 Monoclonal Antibody, Apoptosis • Mouse • Western blot 100 UL 2290-MC-100 clone YTH-10C4 • Rat • Immunoprecipitation Bcl-2 Monoclonal Antibody, Apoptosis • Mouse • Western blot 25 UL 2290-MC-025 clone YTH-10C4 • Rat • Immunoprecipitation Bcl-2 Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 2291-MC-100 clone YTH-8C8 • Immunoprecipitation Bcl-2 Monoclonal Antibody, Apoptosis • Human • Western blot 25 UL 2291-MC-025 clone YTH-8C8 • Immunoprecipitation Bcl-XL Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 2300-MC-100 clone YTH-2H12 • Mouse • Immunoprecipitation • Rat • Immunohistochemistry Bcl-XL Monoclonal Antibody, Apoptosis • Human • Western blot 25 UL 2300-MC-025 clone YTH-2H12 • Mouse • Immunoprecipitation • Rat • Immunohistochemistry Beta Polymerase Enzyme DNA Damage • Multiple • Gap fi lling 1000 UNITS 4020-01K-EB Oxidative Stress • DNA labeling Beta Polymerase Enzyme DNA Damage • Multiple • Gap fi lling 100 UNITS 4020-100-EB Oxidative Stress • DNA labeling Beta Polymerase Enzyme DNA Damage • Multiple • Gap fi lling 500 UNITS 4020-500-EB Oxidative Stress • DNA labeling Beta Polymerase Kit DNA Damage • Multiple • Gap fi lling 100 UNITS 4020-100-K Oxidative Stress • DNA labeling Bid Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2315-PC-050 • Immunoblotting Bim Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2341-PC-050 • Mouse • Immunoblotting Biotinylated NAD Supporting Reagents • Multiple • Substrate 500 UL 4670-500-01 Biotinylated NAD Supporting Reagents • Multiple • Substrate 5 X 500 UL 4670-500-05 www.trevigen.com 29 Description Research Area Species Reactivity Applications Size Catalog No. Biotinylated NAD Supporting Reagents • Multiple • Substrate 10 X 500 UL 4670-500-10 BloxALL™ Biotin Free Blocking Supporting Reagents • Multiple • ELISA 100 ML 2704-100-01 Solution • Western blot • Immunocytochemistry • Immunohistochemistry BloxALL™ Blocking Solution Supporting Reagents • Multiple • ELISA 100 ML 2704-100-02 • Western blot • Immunocytochemistry • Immunohistochemistry New Cultrex® BME HC20+™ PathClear® Cell Behavior • In vivo angiogenesis assays 5 ML 3444-005-02 • Tumorgenicity • Plug assay Cultrex® BME with Phenol Red Cell Behavior • Mammalian • Tube assay 1 ML 3430-001-01 • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME with Phenol Red Cell Behavior • Mammalian • Tube assay 1 ML 3430-001-02 PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME with Phenol Red Cell Behavior • Mammalian • Tube assay 5 ML 3430-005-01 • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME with Phenol Red Cell Behavior • Mammalian • Tube assay 5 ML 3430-005-02 PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME with Phenol Red, Cell Behavior • Mammalian • Tube assay 1 ML 3431-001-01 Reduced Growth Factor • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME with Phenol Red, Cell Behavior • Mammalian • Tube assay 1 ML 3431-001-02 Reduced Growth Factor PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME with Phenol Red, Cell Behavior • Mammalian • Tube assay 5 ML 3431-005-01 Reduced Growth Factor • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME with Phenol Red, Cell Behavior • Mammalian • Tube assay 5 ML 3431-005-02 Reduced Growth Factor PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation

30 1.800.873.8443 2008 TREVIGEN PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. Cultrex® BME without Phenol Red Cell Behavior • Mammalian • Tube assay 1 ML 3432-001-01 • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME without Phenol Red Cell Behavior • Mammalian • Tube assay 1 ML 3432-001-02 PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME without Phenol Red Cell Behavior • Mammalian • Tube assay 5 ML 3432-005-01 • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME without Phenol Red Cell Behavior • Mammalian • Tube assay 5 ML 3432-005-02 PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME without Phenol Red, Cell Behavior • Mammalian • Tube assay 1 ML 3433-001-01 Reduced Growth Factor • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME without Phenol Red, Cell Behavior • Mammalian • Tube assay 1 ML 3433-001-02 Reduced Growth Factor PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® BME without Phenol Red, Cell Behavior • Mammalian • Tube assay 5 ML 3433-005-01 Reduced Growth Factor • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation New Cultrex® BME without Phenol Red, Cell Behavior • Mammalian • Tube assay 5 ML 3433-005-02 Reduced Growth Factor PathClear® • Ring assay • Cell invasion • In vivo and in vitro angiogenesis assays • Tumorgenicity • Cell culture • Cell differentiation Cultrex® 3D Culture MatrixTM BME Cell Behavior • Mammalian • 3D culture 1 ML 3445-001-01 without Phenol Red, Reduced Growth • Differentiation assays Factor • Apoptosis detection Cultrex® 3D Culture MatrixTM BME Cell Behavior • Mammalian • 3D culture 5 ML 3445-005-01 without Phenol Red, Reduced Growth • Differentiation assays Factor • Apoptosis detection Cultrex® 3D Culture MatrixTM BME Cell Behavior • Mammalian • 3D culture 15 ML 3445-048-01 without Phenol Red, Reduced Growth • Differentiation assays Factor • Apoptosis detection BPDE Monoclonal Antibody, DNA Damage • Multiple • ELISA 100 UL 4360-MC-100 clone 8E11 Oxidative Stress • Affi nity chromatography BPDE-DNA Monoclonal Antibody, DNA Damage • Multiple • ELISA 100 UL 4365-MC-100 clone 5D11 Oxidative Stress • Affi nity chromatography

www.trevigen.com 31 Description Research Area Species Reactivity Applications Size Catalog No. Calf Thymus DNA Supporting Reagents • Multiple • Northern blot 5 X 1 ML 9600-5-D • Southern blot • Dot blot Caspase-10/A Monoclonal Antibody, Apoptosis • Human • Western blot 50 UL 2309-MC-050 clone 63131.111 • Mouse Caspase-10/B Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2310-PC-050 • Mouse Caspase-2 Polyclonal Antibody Apoptosis • Human • Western blot 40 UL 2304-PC-040 • Mouse Caspase-3 Polyclonal Antibody Apoptosis • Human • Western blot 100 UL 2305-PC-100 (Active) • Mouse • Immunoprecipitation • Immunohistochemistry Caspase-3 Polyclonal Antibody Apoptosis • Human • Western blot 20 UL 2305-PC-020 (Active) • Mouse • Immunoprecipitation • Immunohistochemistry Caspase-7 Monoclonal Antibody, Apoptosis • Human • Western blot 50 UL 2312-MC-050 clone MCH3-5 Caspase-9 Monoclonal Antibody, Apoptosis • Human • Western blot 50 UL 2308-MC-050 clone LAP6-96 CD95 (Fas) Monoclonal Antibody, Apoptosis • Human • Western blot 250 UL 2343-MC-250 clone DX2 • ELISA • Immunohistochemistry • Apoptosis induction CD95 (Fas) Monoclonal Antibody, Apoptosis • Human • Western blot 250 UL 2344-MC-250 clone 50830.11 • ELISA CD95 (Fas) Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2342-PC-050 • ELISA

® New Cultrex BME Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3455-024-K • Cell invasion assays Cultrex® BME Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 96 WELLS 3455-096-K • Cell invasion assays New Cultrex® BME Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3456-024-K • Cell invasion assays Cultrex® Laminin Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 96 WELLS 3456-096-K • Cell invasion assays New Cultrex® Laminin Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3457-024-K • Cell invasion assays Cultrex® Collagen I Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 96 WELLS 3457-096-K • Cell invasion assays New Cultrex® Collagen I Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3458-024-K • Cell invasion assays Cultrex® Collagen IV Cell Invasion Kit Cell Behavior • Mammalian • Cell migration assays 96 WELLS 3458-096-K • Cell invasion assays New CultureCoat® BME Cell Invasion Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3460-024-K Assay • Cell invasion assays New Cultrex® Endothelial Cell Invasion Cell Behavior • Cell migration assays 96 WELLS 3471-096-K Assay • Cell invasion assays New Cultrex® Cell Migration Kit Cell Behavior • Mammalian • Cell migration assays 24 WELLS 3465-024-K

Cultrex® Cell Migration Kit Cell Behavior • Mammalian • Cell migration assays 96 WELLS 3465-096-K Cultrex® MTT Cell Proliferation Kit Cell Behavior • Mammalian • Cell proliferation 2500 TESTS 4890-025-K • Cytotoxicity Cultrex® MTT Cell Proliferation Kit Cell Behavior • Mammalian • Cell proliferation 5000 TESTS 4890-050-K • Cytotoxicity Cultrex® XTT Cell Proliferation Kit Cell Behavior • Mammalian • Cell proliferation 2500 TESTS 4891-025-K • Cytotoxicity Cultrex® Cell Staining Kit Cell Behavior • Mammalian • Tube assay 200 TESTS 3437-100-K • Neurite outgrowth assay • Ring assay Cultrex® Cell Viability Kit Cell Behavior • Mammalian • Viability and Fluorescent cytotoxicity 1000 TESTS 4892-010-K

32 1.800.873.8443 2008 TREVIGEN PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. cIAP Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2319-PC-050 • Mouse Collagen I Polyclonal Antibody Cell Behavior • Mouse • Western blot 100 UL 3440-PC-100 • Rat • ELISA • Immunohistochemistry • Immunocytochemistry Cultrex® 3D Culture MatrixTM Cell Behavior • Mammalian • 3D culture assays 100 MG 3447-020-01 Collagen I Protein New Cultrex® 3D Culture Cell Proliferation Cell Behavior • Mammalian • 3D culture 96 TESTS 3445-096-CK Assay Core Kit • Cell proliferation assays • Drug screening New Cultrex® 3D Culture BME Cell Cell Behavior • Mammalian • 3D culture 96 TESTS 3445-096-K Proliferation Assay Kit • Cell proliferation assays • Drug screening New Cultrex® 3D Culture Laminin I Cell Cell Behavior • Mammalian • 3D culture 96 TESTS 3446-096-K Proliferation Assay Kit • Cell proliferation assays • Drug screening New Cultrex® 3D Culture Collagen I Cell Cell Behavior • Mammalian • 3D culture 96 TESTS 3447-096-K Proliferation Assay Kit • Cell proliferation • Drug screening New Cultrex® 3D Culture Cell Harvesting Cell Behavior • Human • 3D culture 96 TESTS 3448-020-K Kit • Mouse • Cell harvesting Cultrex® Collagen I Protein Cell Behavior • Mammalian • Cell culture 50 MG 3442-050-01 • Cell attachment • Cell differentiation • Cell migration assays • Cell invasion assays Cultrex® Collagen I Protein Cell Behavior • Mammalian • Cell culture 100 MG 3440-100-01 • Cell attachment • Cell differentiation • Cell migration assays • Cell invasion assays Cultrex® Collagen IV Protein Cell Behavior • Mammalian • Cell culture 1 MG 3410-010-01 • Cell attachment • Cell differentiation • Cell migration assays • Cell invasion assays Cv-PDG Enzyme DNA Damage • Multiple • DNA nicking 1000 UNITS 4065-100-EB Oxidative Stress Cyclic AMP Competitive ELISA Kit DNA Damage • Multiple • Competitive ELISA 96 WELLS 2500-096-K Oxidative Stress Cyclic AMP Competitive ELISA Kit DNA Damage • Multiple • Competitive ELISA 96 WELLS 2501-096-K Oxidative Stress Cytochrome C Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 6370-MC-100 clone 4CYTC-21 • Mouse • Rat • Bovine • Equine • Porcine Cytonin Supporting Reagents • Mammalian • Permeabilization 10 ML 4876-05-02 CometAssay™ DNA Damage Kit DNA Damage • Multiple • Comet assay 10 ASSAYS 4256-010-CC Oxidative Stress CometAssay™ DNA Damage Kit DNA Damage • Multiple • Comet assay 40 SAMPLES 4252-040-K Oxidative Stress CometAssay™ DNA Damage Kit DNA Damage • Multiple • Comet assay 50 SAMPLES 4250-050-K Oxidative Stress CometAssay™ DNA Damage Kit DNA Damage • Multiple • Comet assay 96 SAMPLES 4253-096-K Oxidative Stress CometAssay™ DNA Damage Kit DNA Damage • Multiple • Comet assay 50 SAMPLES 4251-050-K Oxidative Stress CometAssay™ LMAgarose DNA Damage Not Applicable • Comet and FLARE (Fragment Length 15 ML 4250-050-02 Oxidative Stress Analysis using Repair Enzymes) assay CometAssay™ LMAgarose DNA Damage Not Applicable • Comet and FLARE (Fragment Length 100 ML 4250-500-02 Oxidative Stress Analysis using Repair Enzymes) assay CometAssay™ Staining Kit Apoptosis • Multiple • Comet assay staining 200 TESTS 4254-200-K www.trevigen.com 33 Description Research Area Species Reactivity Applications Size Catalog No. DASHTM DNA Damage Kit DNA Damage • Multiple • DNA damage detection and quantitation 50 SAMPLES 4255-050-K Oxidative Stress • Apoptosis detection CometSlide™ Slide Rack System DNA Damage Not Applicable • Comet assay 1 RACK 4252-040-02 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 100 SLIDES 4252-02K-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 2 SLIDES 4252-040-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 10 SLIDES 4252-200-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 25 SLIDES 4252-500-01 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 2 SLIDES 4250-004-03 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 25 SLIDES 4250-050-03 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 100 SLIDES 4250-200-03 Oxidative Stress CometSlide™ Slides (96-Well) DNA Damage Not Applicable • Comet assay 20 SLIDES 4253-02K-03 Oxidative Stress CometSlide™ Slides (96-Well) DNA Damage Not Applicable • Comet assay 1 SLIDE 4253-096-03 Oxidative Stress CometSlide™ Slides (96-Well) DNA Damage Not Applicable • Comet assay 100 SLIDES 4253-10K-03 Oxidative Stress CometSlide™ Slides (96-Well) DNA Damage Not Applicable • Comet assay 10 SLIDES 4253-960-03 Oxidative Stress FLARE™ FPG DNA Damage Kit DNA Damage • Multiple • FLARE (Fragment Length Analysis using 75 SAMPLES 4040-100-FK Oxidative Stress Repair Enzymes) FLARE™ Endonuclease III DNA DNA Damage • Multiple • FLARE (Fragment Length Analysis using 75 SAMPLES 4045-01K-FK Damage Kit Oxidative Stress Repair Enzymes) FLARE™ cv-PDG DNA Damage Kit DNA Damage • Multiple • FLARE (Fragment Length Analysis using 75 SAMPLES 4065-100-FK Oxidative Stress Repair Enzymes) FLARE™ UVDE DNA Damage Kit DNA Damage • Multiple • FLARE (Fragment Length Analysis using 75 SAMPLES 4100-100-FK Oxidative Stress Repair Enzymes) FLARE™ hOGG1 DNA Damage Kit DNA Damage • Multiple • FLARE (Fragment Length Analysis using 75 SAMPLES 4130-100-FK Oxidative Stress Repair Enzymes) FLARE™ T4-PDG DNA Damage Kit DNA Damage • Multiple • Comet assay 75 SAMPLES 4055-100-FK Oxidative Stress • FLARE (Fragment Length Analysis using Repair Enzymes) FLARE™ Slides (3-Well) DNA Damage Not Applicable • FLARE (Fragment Length Analysis using 25 SLIDES 3950-075-02 Oxidative Stress Repair Enzymes) assay FLARE™ Slides (3-Well) DNA Damage Not Applicable • FLARE (Fragment Length Analysis using 100 SLIDE 3950-300-02 Oxidative Stress Repair Enzymes) assay DNA Ligase IV/XRCC4 Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4140-100-EB Oxidative Stress DNA Ligase IV/XRCC4 Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4140-500-EB Oxidative Stress DPO4 Enzyme DNA Damage • Multiple • PCR additive 400 UNITS 4150-010-EB Oxidative Stress DPO4 Enzyme DNA Damage • Multiple • PCR additive 2000 UNITS 4150-050-EB Oxidative Stress EGF Protein Cell Behavior • Human • Cell Culture 50 UG 3443-050-01 Endonuclease III Enzyme DNA Damage • Multiple • DNA nicking 1000 UNITS 4045-01K-EB Oxidative Stress Endonuclease III Enzyme DNA Damage • Multiple • DNA nicking 5000 UNITS 4045-05K-EB Oxidative Stress Endonuclease IV Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4050-100-EB Oxidative Stress Endonuclease IV Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4050-500-EB Oxidative Stress

34 1.800.873.8443 2008 TREVIGEN PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. Endonuclease V Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4035-500-EB Oxidative Stress Endonuclease VIII Enzyme DNA Damage • Multiple • DNA nicking 1000 UNITS 4060-01K-EB Oxidative Stress Endonuclease VIII Enzyme DNA Damage • Multiple • DNA nicking 5000 UNITS 4060-05K-EB Oxidative Stress Etoposide Supporting Reagents • Multiple • Apoptosis induction 400 UL 4886-400-01 Exonuclease III Enzyme DNA Damage • Multiple • DNA nicking 5000 UNITS 4030-05K-EB Oxidative Stress Fen-1 Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4120-100-EB Oxidative Stress Fen-1 Polyclonal Antibody DNA Damage • Human • Western blot 100 UL 4410-PC-100 Oxidative Stress • Mouse • Neutralization FGF- 2 Protein Cell Behavior • Human • Cell culture 5 UG 3443-050-02 Fibronectin Polyclonal Antibody Cell Behavior • Rat • Western blot 100 UL 3416-PC-100 • Bovine • ELISA • Immunohistochemistry • Immunocytochemistry Fibronectin Protein Cell Behavior • Mammalian • Cell Culture 1 MG 3416-001-01 FITC-NAD Supporting Reagents • Multiple • Substrate 250 UL 4673-500-01 Fpg Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4040-100-EB Oxidative Stress Fpg Enzyme DNA Damage • Multiple • DNA nicking 2500 UNITS 4040-500-EB Oxidative Stress G3PDH Polyclonal Antibody Apoptosis • Human • Western blot control 100 UL 2275-PC-100 • Mouse • Immunoprecipitation Genomic DNA Isolation Kit Supporting Reagents • Multiple • DNA isolation 20 TESTS 4850-20-GD Glutathione Kit DNA Damage • Multiple • Activity assay 384 SAMPLES 7511-100-K Oxidative Stress Glutathione Peroxidase Activity Kit DNA Damage • Multiple • Activity assay 384 SAMPLES 7512-100-K Oxidative Stress Glutathione Reductase Activity Kit DNA Damage • Multiple • Activity assay 384 SAMPLES 7510-100-K Oxidative Stress Glutathione Reductase Activity Kit DNA Damage • Multiple • Activity assay 384 SAMPLES 7513-500-K Oxidative Stress H2AX Polyclonal Antibody DNA Damage • Mammalian • Western blot 100 UL 4411-PC-100 Oxidative Stress • yeast • Neutralization • D. melanogaster • X. laevis Herring Sperm DNA Supporting Reagents Not Applicable • Northern blot 5 X 1 ML 9605-5-D • Southern blot • Dot blot Histone Coated Plate DNA Damage • Multiple • PARP assay 96 WELLS 4677-096-P Oxidative Stress Histone Coated Plate DNA Damage • Multiple • PARP assay 96 WELLS 4678-096-P Oxidative Stress hOGG1 Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4130-100-EB Oxidative Stress hOGG1 Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4130-500-EB Oxidative Stress Holocytochrome C Monoclonal Apoptosis • Human • Western blot 100 UL 6380-MC-100 Antibody, clone 2CYTC-199 • Mouse • ELISA • Rat • Immunoprecipitation New Homogeneous PARP Inhibition Assay DNA Damage • PARP activity assay 96 WELLS 4690-096-K • Inhibitor screening Ku 70/80 Enzyme DNA Damage • Multiple • Double stranded DNA break detection 250U 4135-250-01 Oxidative Stress Laminin I Polyclonal Antibody Cell Behavior • Mouse • Western blot 100 UL 3400-PC-100 • Rat • ELISA • Immunohistochemistry • Immunocytochemistry

www.trevigen.com 35 Description Research Area Species Reactivity Applications Size Catalog No. Cultrex® 3D Culture MatrixTM Laminin Cell Behavior • Mammalian • 3D culture reagent 5 ML 3446-005-01 I Protein • Differentiation assay Laminin Protein Cell Behavior • Mammalian • Cellular adhesion 1 MG 3400-010-01 • Migration • Invasion assay Loading Buffer Supporting Reagents Not Applicable • Western blot reagent 1500 UL 9850-250-6 • Electrophoresis Lysis Solution DNA Damage Not Applicable • Comet and FLARE assay 2 X 500 ML 4250-050-01 Oxidative Stress Mcl-1 Polyclonal Antibody Apoptosis • Human • Immunoprecipitation 75 UL 2316-PC-075 • Western blot Mcl-1 Protein Apoptosis • Human • Western blot standard 50 UL 2316-050-01 Microscope Slides DNA Damage Not Applicable • Histochemistry 100 SLIDES 4861-100 Oxidative Stress Mounting Medium Apoptosis Not Applicable • Histochemistry 25 ML 4865-25 Mounting Medium Apoptosis Not Applicable • Histochemistry 20 ML 4866-20 MUG Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4125-100-EB Oxidative Stress MutY Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4000-500-EB Oxidative Stress Neuropore • Multiple • Permeabilization 10 ML 4820-60-01 PAR Monoclonal Antibody, DNA Damage • Multiple • Western blot 50 UL 4335-AMC-050 clone 10HA Oxidative Stress • ELISA PAR Monoclonal Antibody, DNA Damage • Multiple • Western blot 100 UL 4335-MC-100 clone 10HA Oxidative Stress • ELISA PAR Monoclonal Antibody, DNA Damage • Multiple • Western blot 1000 UL 4335-MC-1K clone 10HA Oxidative Stress • ELISA PAR Polyclonal Antibody DNA Damage • Multiple • Western blot 50 UL 4336-APC-050 Oxidative Stress PAR Polyclonal Antibody DNA Damage • Multiple • Western blot 100 UL 4336-BPC-100 Oxidative Stress PAR Polymer DNA Damage • Multiple • ELISA control 100 UL 4336-100-01 Oxidative Stress PAR Protein DNA Damage • Multiple • Western blot 10 UL 4500-10-P Oxidative Stress • ELISA control PARG Assay Kit DNA Damage • Multiple • PARG activity 96 WELLS 4682-096-K Oxidative Stress • Inhibitor screening PARG Assay Kit DNA Damage • Multiple • PARG activity 96 WELLS 4683-096-K Oxidative Stress • Inhibitor screening PARP Activity Kit DNA Damage • Multiple • PARP activity 96 WELLS 4671-096-K Oxidative Stress • Inhibitor screening PARP Activity Kit DNA Damage • Multiple • PARP activity 96 WELLS 4675-096-K Oxidative Stress • Inhibitor screening PARP Activity Kit DNA Damage • Multiple • PARP activity 96 WELLS 4676-096-K Oxidative Stress • Inhibitor screening PARP Activity Kit DNA Damage • Multiple • PARP activity 96 WELLS 4677-096-K Oxidative Stress • Inhibitor screening PARP Apoptosis Assay Apoptosis • Multiple • Apoptosis detection 96 WELLS 4684-096-K • PARP activity PARP Apoptosis Assay Apoptosis • Multiple • Apoptosis detection 96 WELLS 4685-096-K • PARP activity PARP Enzyme DNA Damage • Multiple • PARP activity 1 ML 4667-1K-01 Oxidative Stress • Inhibitor screening PARP Enzyme DNA Damage • Multiple • PARP activity 250 UL 4667-250-01 Oxidative Stress • Inhibitor screening PARP Enzyme DNA Damage • Multiple • PARP activity 50 UL 4667-50-01 Oxidative Stress • Inhibitor screening PARP Enzyme (HSA) DNA Damage • Multiple • PARP activity 1000 UNITS 4668-100-01 Oxidative Stress • Inhibitor screening

36 1.800.873.8443 2008 TREVIGEN PRICE LIST

Description Research Area Species Reactivity Applications Size Catalog No. PARP Enzyme (HSA) DNA Damage • Multiple • PARP activity 20,000 UNITS 4668-2K-01 Oxidative Stress • Inhibitor screening PARP Enzyme (HSA) DNA Damage • Multiple • PARP activity 5000 UNITS 4668-500-01 Oxidative Stress • Inhibitor screening PARP Monoclonal Antibody, DNA Damage • Human • Western blot 50 UL 4338-MC-50 clone C2-10 Oxidative Stress • Mouse • Immunocytochemistry • Rat • Monkey • Hamster PBR Polyclonal Antibody Apoptosis • Human • Western blot 100 UL 6361-PC-100 • Mouse • Immunohistochemistry • Rat PBR Polyclonal Antibody Apoptosis • Mouse • Western blot 100 UL 6362-PC-100 • Immunohistochemistry PBS Buffer Supporting Reagents Not Applicable • Buffer 1000 ML 4868-500-06 PBS Buffer Supporting Reagents Not Applicable • Buffer 500 ML 4870-500 PBS Buffer Supporting Reagents Not Applicable • Buffer 3000 ML 4870-500-6 Photolyase Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4145-100-EB Oxidative Stress Photolyase Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4145-500-EB Oxidative Stress Cultrex® Poly-L-Lysine Cell Behavior • Multiple • Cell adhesion 100 ML 3438-100-01 Proteinase K Apoptosis • Multiple • Permeabilization 30 UL 4800-30-01 Rad1 Monoclonal Antibody, DNA Damage • Human • Western blot 200 UL 4451-MC-200 clone 33 Oxidative Stress • Mouse Salmon Sperm DNA Supporting Reagents Not Applicable • Northern blot 5 X 1 ML 9610-5-D • Southern blot • Dot blot CometSlide™ Slide Rack System DNA Damage Not Applicable • Comet assay 1 RACK 4252-040-02 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 100 SLIDES 4252-02K-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 2 SLIDES 4252-040-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 10 SLIDES 4252-200-01 Oxidative Stress CometSlide™ Slides (20-Well) DNA Damage Not Applicable • Comet assay 25 SLIDES 4252-500-01 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 2 SLIDES 4250-004-03 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 25 SLIDES 4250-050-03 Oxidative Stress CometSlide™ Slides (2-Well) DNA Damage Not Applicable • Comet assay 100 SLIDES 4250-200-03 Oxidative Stress FLARE™ Slides (3-Well) DNA Damage Not Applicable • FLARE (Fragment Length Analysis using 25 SLIDES 3950-075-02 Oxidative Stress Repair Enzymes) assay FLARE™ Slides (3-Well) DNA Damage Not Applicable • FLARE (Fragment Length Analysis using 100 SLIDE 3950-300-02 Oxidative Stress Repair Enzymes) assay CometSlide™ Slides (96-Well) DNA Damage Not Applicable • Comet assay 20 SLIDES 4253-02K-03 Oxidative Stress CometSlide™ Slides 96-Well DNA Damage Not Applicable • Comet assay 1 SLIDE 4253-096-03 Oxidative Stress CometSlide™ Slides 96-Well DNA Damage Not Applicable • Comet assay 100 SLIDES 4253-10K-03 Oxidative Stress CometSlide™ Slides 96-Well DNA Damage Not Applicable • Comet assay 10 SLIDES 4253-960-03 Oxidative Stress SOD Activity Kit DNA Damage • Multiple • Activity assay 100 TESTS 7500-100-K Oxidative Stress SOD Activity Kit DNA Damage • Multiple • Activity assay 480 TESTS 7501-500-K Oxidative Stress

www.trevigen.com 37 Description Research Area Species Reactivity Applications Size Catalog No. Staurosporine Apoptosis • Multiple • Apoptosis induction 100 UL 4886-100-02 T4 Endonuclease V Enzyme DNA Damage • Multiple • DNA nicking 100,000 UNITS 4055-100-EB Oxidative Stress TDG Enzyme DNA Damage • Multiple • DNA nicking 500 UNITS 4070-500-EB Oxidative Stress TDG Kit DNA Damage • Multiple • Mismatch detection 500 UNITS 4070-500-K Oxidative Stress TRAIL Polyclonal Antibody Apoptosis • Human • Western blot 50 UG 2317-PC-050 • ELISA • Neutralization UNGase Enzyme DNA Damage • Multiple • DNA nicking 100 UNITS 4025-100-EB Oxidative Stress UVDE Enzyme DNA Damage • Multiple • DNA nicking 100 UL 4100-100-EB Oxidative Stress UVssDNA Monoclonal Antibody, DNA Damage • Multiple • ELISA 100 UL 4350-MC-100 clone C3B6 Oxidative Stress • DNA damage detection (colorimetric) Valinomycin Apoptosis • Multiple • Apoptosis induction 100 UL 6305-100-02 VEGF Protein Cell Behavior • Human • Angiogenesis stimulant 1 UG 3443-050-03 Vitronectin Polyclonal Antibody Cell Behavior • Bovine • Western blot 100 UL 3417-PC-100 • ELISA • Immunohistochemistry • Immunocytochemistry Vitronectin Protein Cell Behavior • Bovine • Cell culture 50 UG 3417-001-01 XIAP Polyclonal Antibody Apoptosis • Human • Western blot 50 UL 2323-PC-050 • Mouse • Rat XIAP Protein Apoptosis • Human • Western blot standard 50 UG 2320-050-01 • Mouse • Rat XPF Monoclonal Antibody, DNA Damage • Human • Western blot 100 UL 4431-MC-100 clone 218 Oxidative Stress XRCC1 Monoclonal Antibody, Apoptosis • Human • Western blot 100 UL 4421-MC-100 clone 144 PRICES ARE ACCURATE AT TIME OF PRINTING

HT Colorimetric PARG Assay Kit, Catalog No. 4683-096-K For more information please see page 14.

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