BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS • CELL INVASION ASSAYS • DIRECTED IN VIVO ANGIOGENESIS ASSAYS • COMETASSAYTM • TREVIGEN PAR/PARP/PARG • PRODUCT FOCUS and price list 2008-2009MAY 2008 TACSTM IN SITU APOPTOSIS DETECTION KITS • 1.800.TREVIGEN • www.trevigen.com 2008 TREVIGEN PRODUCT FOCUS AND PRICE LIST Product Overviews BME Tools for 3D Culture Assays TACS™ Basement Membrane Extract Products and 3D Culture Cell Proliferation Assay (96 well) .......10 In Situ Apoptosis Detection Kits .....................18-23 Specialty Proteins ............................................2-5 3D Culture Cell Harvesting Kit ............................11 Annexin DIVAA™ PAR/PARP/PARG For monitoring apoptosis via flow cytometry ...24-25 Directed in vivo Angiogenesis Assays .................6-7 Kits, Reagents, Antibodies, and Enzymes .......12-14 Oxidative Stress Kits Cell Invasion/Migration Assays CometAssay™ ..................................................................26-27 Kits, Reagents, and Plates ................................8-9 Kits, Reagents, and Slides ............................15-17 Trevigen Product International Cell Assays ......................................25 Price List ..........................................28-38 Distributors ..............................39-40 Ordering Information • Please use product names and catalog numbers when ordering. Conditions Trevigen products are sold for Research Use Only. • Please provide your name, purchase order number, shipping address, billing address, and telephone number on each order. Warranty • Trevigen accepts VISA, MASTERCARD, and AMERICAN EXPRESS. Trevigen warrants that products will perform according to specifications accompanying each product. Products found not meeting specifications will be replaced, free of charge. Trevigen’s liability is limited to • Fax, phone, e-mail, or mail your order to: replacement of the product. Product discrepancies must be reported Trevigen, Inc. within 5 days of receipt of order. 8405 Helgerman Court Gaithersburg, MD 20877 1-800-873-8443 • Fax: 301-560-4973 Other Local Phone: 301-216-2800 Prices are subject to change. Trevigen reserves the right to change e-mail: [email protected] product specifications. Products may vary somewhat from the catalog description. • Order online at www.trevigen.com Shipments All shipments are made through our carrier unless otherwise requested. Shipping charges are prepaid and added to your invoice. For RUSH orders, please advise Trevigen’s Customer Service Representative at the time of order. Trademarks Trademarks: Trevigen, Cultrex, TACS•XL, SNIPase, CultreCoat, and PathClear are registered trademarks, and 3D Culture Matrix, TACS, AngioRack, Apoptosis Grade, BloxALL, Cytonin, Cytonin IHC, DASH, DIVAA, NeuroPore, PeroxyGlow, TumorTACS, FlowTACS, CardioTACS, TiterTACS, TACS-Sapphire, NeuroTACS, DermaTACS, VasoTACS, TACS-Nuclease, TACS Blue Label, DePsipher, MitoShift, CometAssay, CometSlide, FLARE, REC, CellSperse, HC20+, and TreviGel are trademarks of Trevigen, Inc. TACS: Trevigen Apoptotic Cell System ©2008 Trevigen, Inc. All rights reserved. www.trevigen.com 1 CULTREX® BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS Basement membranes are continuous sheets underneath. This is achieved by cell-matrix Functions of Basement Membranes of specialized extracellular matrix that are adhesions through cell adhesion molecules • Anchor epithelium to underlying stroma found at the base of all lumen-lining epithelial (CAMs). The basement membrane acts as • Mechanical barrier to cell invasion and endothelial cells. They are composed of a mechanical barrier, preventing malignant • Separates two different tissue types specialized basement membrane proteins, cells from invading the deeper tissues. The • Promotes and maintains cell differentiation including collagen IV (the primary structural basement membrane is also essential for • Filtration element of the basement membrane), angiogenesis. Basement membrane proteins • Storage depot for growth factors laminin I, heparan sulfate proteoglycan, have been found to accelerate differentiation and entactin. The primary function of the of endothelial cells. They also play an basement membrane is to anchor the important role in glomerular filtration in the epithelium to its loose connective tissue kidney. Basement Membrane Components Fibrillar collagen matrix (collagen I) Type VI collagen Basement membrane Collagen IV Laminin Sarcolemma Integrin Growth Factor Receptors Dystroglycan- Dystrophin Sarcoglycan complex 2 1.800.873.8443 BASEMENT MEMBRANE EXTRACT PRODUCTS AND SPECIALTY PROTEINS CULTREX® BASEMENT MEMBRANE EXTRACT PRODUCTS BME PRODUCTS Multipurpose Cultrex® BME (Basement Membrane Extract) Multipurpose Cultrex® BME (Basement Membrane Extract) Description Size Catalog No. ® 5 ml 3430-005-01 Multipurpose BME is available in BME types and our specialty BME matrices Cultrex BME with Phenol Red concentrations ranging from 12-17 mg/ are available in trial sizes for your testing Cultrex® BME with Phenol Red, 5 ml 3431-005-01 Reduced Growth Factor ml and is qualified for angiogenesis, convenience. BME lots can be reserved for Cultrex® BME, No Phenol Red 5 ml 3432-005-01 tumorigenicity assays, migration, growth, your research requirements. Each lot of BME ® differentiation, and neurite outgrowth has passed sterility testing following USP Cultrex BME, No Phenol Red, 5 ml 3433-005-01 Reduced Growth Factor assays. It is available either reduced or XXIV Chapter 71 sterility test and is negative non-reduced in growth factors and either for mycoplasma by PCR. Cultrex® BME PathClear® with phenol red or without. All four standard Description Size Catalog No. Cultrex® BME with Phenol Red, 5 ml 3430-005-02 Cultrex® BME PathClear® PathClear® Cultrex® BME with Phenol Red, 5 ml 3431-005-02 ® BME PathClear is sterile for bacterial and mycoplasma as well as 31 other pathogens Reduced Growth Factor fungal growth according to USP XXIV, Chapter and viruses including LDEV. Patent applied PathClear® 71. It has been tested and cleared for for. Cultrex® BME, No Phenol Red, 5 ml 3432-005-02 PathClear® Cultrex® BME, No Phenol Red, 5 ml 3433-005-02 Cultrex® 3D Culture Matrix™ Reduced Growth Factor PathClear® Trevigen provides three different matrices Laminin I and the 3D Culture Matrix™ capable of forming 3D substrates for cell Collagen I may be used as a gel on which Cultrex® 3D Culture Matrix™ culture and differentiation. The 3D Culture to grow cells or a media additive alone or Description Size Catalog No. Matrix™ BME is our reduced growth factor in concert with other basement membrane Cultrex® 3D BME, No Phenol 15 ml 3445-048-01 BME qualified, lot-to-lot, to promote components to study cellular growth and Red, Reduced Growth Factor differentiation of human epithelial cell lines differentiation in three dimensions in vitro. Cultrex® 3D Laminin I 5 ml 3446-005-01 derived from mammary gland (MCF-10A) 3D Culture Matrix™ Laminin I is available Cultrex® 3D Collagen I - 20 ml 3447-020-01 and human prostate (PC-3) into acinar at a consistent concentration of 6 mg/ml Rat Tail structures. This matrix provides the foundation (by absorbance and extinction co-efficient) ® ® for cells to grow in three dimensions allowing (BCA) and the 3D Culture Matrix™ Collagen Cultrex HC20+™ BME PathClear for the formation of structures in vitro and is I at 5 mg/ml (Sircol Assay). Description Size Catalog No. available at a consistent concentration range Cultrex® High Protein BME 5 ml 3444-005-02 of 12-17 mg/ml. The 3D Culture Matrix™ (HC20+™) PathClear® SPECIALTY PROTEINS Cultrex® HC20+™ BME PathClear® Description Size Catalog No. ® ® Cultrex HC20+™ BME is available at PathClear has been tested and cleared of Cultrex® Bovine Collagen I 50 mg 3442-050-01 a concentration of 20 mg/ml or greater 31 pathogens and viruses including LDEV Cultrex® Rat Collagen I 100 mg 3440-100-01 for those requiring a high concentration (Lactate Dehydrogenase Elevating Virus) Cultrex® Mouse Collagen IV 1 mg 3410-010-01 BME for in vivo angiogenesis assays making it ideal for in vivo work. Patent ® and tumorigenicity assays. Cultrex® BME pending. Patent applied for. Cultrex Mouse Laminin I 1 mg 3400-010-01 Cultrex® Bovine Fibronectin 1 mg 3416-001-01 ® 50 μg 3417-001-01 CITATIONS Cultrex Bovine Vitronectin Interleukin-6 is a potent growth factor for ER--positive human breast cancer ACCESSORY PRODUCTS A. Kate Sasser, Nicholas J. Sullivan, Adam W. Studebaker, Lindsay F. Hendey, Amy E. Axel, and Brett M. Hall FASEB J, Jun 2007; 21: 3763 - 3770. Description Size Catalog No. Cultrex® Poly-L-Lysine 100 ml 3438-100-01 Identifi cation of proangiogenic TIE2-expressing monocytes (TEMs) in human peripheral blood and cancer Mary Anna Venneri, Michele De Palma, Maurilio Ponzoni, Ferdinando Pucci, Cristina Scielzo, Erika Zonari, Roberta Mazzieri, Claudio Doglioni, and Luigi Naldini Blood, Jun 2007; 109: 5276 – 5285. www.trevigen.com 3 SPECIALTY PROTEINS BME PRODUCTS Trevigen offers a range of specialty proteins including Mouse Laminin I, Mouse Collagen IV, ® Multipurpose Cultrex BME (Basement Membrane Extract) Rat Collagen I, Bovine Collagen I, Bovine Fibronectin and Bovine Vitronectin. Description Size Catalog No. Cultrex® BME with Phenol Red 5 ml 3430-005-01 Collagen I Cultrex® BME with Phenol Red, 5 ml 3431-005-01 Reduced Growth Factor Collagen I is the major structural component helix scaffold when at a neutral pH and Cultrex® BME, No Phenol Red 5 ml 3432-005-01 of extracellular matrices found in connective 37°C. Collagen I promotes