RESEARCH ARTICLE A Comprehensive and Comparative Study of Wolfiporia extensa Cultivation Regions by Fourier Transform Infrared Spectroscopy and Ultra-Fast Liquid Chromatography Yan Li1,2, Ji Zhang1,2, Tao Li3, Honggao Liu4*, Yuanzhong Wang1,2* 1 Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China, 2 Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, Yunnan, China, 3 College of Resources and Environment, Yuxi Normal University, Yuxi, Yunnan, China, 4 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, China a1111111111 a1111111111 *
[email protected] (HL);
[email protected] (YW) a1111111111 a1111111111 a1111111111 Abstract Nowadays, Wolfiporia extensa as a popular raw material in food and medicine industry has received increasing interests. Due to supply shortage, this species of edible and medicinal mushroom has been cultivated in some provinces of China. In the present study, cultivated OPEN ACCESS W. extensa collected from six regions in Yunnan Province of China were analyzed by an Citation: Li Y, Zhang J, Li T, Liu H, Wang Y (2016) integrated method based on Fourier transform infrared (FT-IR) spectroscopy and ultra-fast A Comprehensive and Comparative Study of Wolfiporia extensa Cultivation Regions by Fourier liquid chromatography (UFLC) coupled with multivariate analysis including partial least Transform Infrared Spectroscopy and Ultra-Fast squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) in order to Liquid Chromatography. PLoS ONE 11(12): investigate the differences and similarities in different origins and parts. In the tested mush- e0168998. doi:10.1371/journal.pone.0168998 room samples, characteristic FT-IR spectra were obtained for acquiring comprehensive Editor: Vijai Gupta, National University of Ireland - fuzz chemical information and pachymic acid was determinated as a biomarker in the mean- Galway, IRELAND time.