ANNUAL REPORT JULY 2011- JUNE 2012

SEAMEO TROPMED REGIONAL CENTRE FOR MICROBIOLOGY, PARASITOLOGY AND ENTOMOLOGY INSTITUTE FOR MEDICAL RESEARCH KUALA LUMPUR, MALAYSIA

1. Introduction

The Institute for Medical Research (IMR) is one of the network of research institutions under National Institute of Health (NIH), Ministry of Health Malaysia. The NIH identifies research priorities areas through conduct of research, leading to utilization and translation of research findings into new knowledge, development of better tools for the diagnosis and management of important health problems, health policy formulation, health management, and health promotion. IMR main function is to carry out research to identify, elucidate, control and prevent diseases and health issues prevalent in the country. Its mission is to improve health by 1) carrying out quality biomedical research to address national health priorities, 2) providing specialized diagnostic services and 3) building national capacity through technology transfer and consultative services. IMR also provide specialized diagnostic services, training in specialized fields and consultative /advisory services to health providers, managers and planners in improving the health of the Malaysian population. IMR’s vision is to be recognised internationally by the scientific community as the leading centre for biomedical research. IMR Research programmes are geared towards these various identified priority areas by NIH and in line with the health problems of the country.

1 Health Research Priorities in Malaysia for the10th Malaysia Plan (2011 – 2015), is to improve the health and quality of life of the people of Malaysia. This, in turn, would enhance their productivity and competitiveness, thereby contributing the economic development. Research findings and outputs are used by managers and administrators of Minsitry of Health Malaysia, other Ministry and government agencies which are related to health to improve the health status, promote wellness and to reduce the burden of disease among Malaysians. This is towards becoming advanced nation’ status by 2020. IMR, one of the research institute under the NIH, will contribute to this vision. IMR consisted of seven Research Centres (Allergy and Immunology, Cancer, Cardiovascular, Diabetes and Nutrition, Environmental Health, Herbal Medicine and Infectious Diseases Research Centre) focusing on biomedical and clinical research, and supported by one Diagnostic Centre. The IMR has 805 posts, of which 638 (79.25%) have been filled. There are 12 individuals in the Senior Management group, 208 individuals in the Managerial and Professional Group, comprising scientists, doctors, dentists, veterinarians, librarians, system analysts, a statistician, a pharmacist and administrative officers. The rest of the staff i.e. 418 persons belong to the Technical and Support group. The total expenditure of the Institute in 2011 was about RM 83.8 million.

2. SEAMEO TROPMED Network Malaysia SEAMEO TROPMED Network Malaysia is one of the international affiliation of the Institute. The Network serve as the Regional Centre for Microbiology, Parasitology and Entomology, where one of the function is to facilitate the strengthening of national and institutional capabilities in research and human resource development through various training and postgraduate degree programmes, linkages, personnel exchanges, scientific forums, technical consultant services, publications and information dissemination. SEAMEO TROPMED Network Malaysia, conduct two regular post graduate Diploma

2 courses each year; the Diploma in Medical Microbiology (DMM) and the Diploma in Applied Parasitology and Entomology (D.A.P&E).

2.1 Academic Programmes

In 2010, both courses undergoes reorganization in terms of schedule and sylybus such that they can be conducted within a year. In 2011 the DMM course was conducted from March to July followed by the DAP&E from August to December. Both were conducted for a duration of 5 months each. The courses were designed to train Medical Doctors, Scientists and other health personnel in basic and advance Medical Microbiology, Parasitology and Entomology, as well as skills in conducting research, diagnostic services and approaches in control programmes.

2.1.1 DMM

The course aims to provide knowledge and practical training on pathogens of major significance to public health and to enable participants to apply scientific approach and critical thinking to diagnose, prevent and control infectious diseases. The course will facilitate and strengthen institutional and national capabilities in medical diagnostic laboratory services, research and human resource development. It is also hoped that this course will encourage networking between the various participants’ institutions and ours to foster exchange in expertise and collaboration in research projects. In 2012, the course was conducted from 13 February to 13 July 2012 with 13 participants coming from one each from Lao PDR, Vietnam, Myanmar and Cambodia, two from Sri Lanka and 7 from Malaysia. Each student conducted an assignment on topics related to the subject of the module, they are as listed in Appendix 1. All the students passed the examination and Mrs. Nilanthi Perera Medical Laboratory Technologist from the Microbiology Laboratory, Service Lab Section, National Institute of Health Sciences, Nagoda, Kalutara, Sri Lanka was awarded the best student for the year 2012.

3 2.1.2 DAP&E

The Course is designed to train doctors, scientists and other health personnel for research, diagnostic services, control programmes and teaching institutions in Applied Parasitology and Entomology. The course is conducted yearly. In 2011, the 42th Course, was conducted from 1st August to 30th December 2011, attended by 16 candidates from 7 countries, one each from Cambodia, Myanmar, Lao PDR and Vietnam, four from Solomon Island, two from Vanuatu and six from Malaysia. The SEAMEO-TROPMED sponsored 7 candidates, 1 each from Myanmar, Lao PDR, Vietnam, Cambodia and 3 from Malaysia. The GLOBAL FUND sponsored 4 candidates from Solomon Islands and the Australian Aid sponsored 2 students from Vanuatu. IMR sponsored 3 students from the Institute. As a fulfillment of the course, each student conducted a research projects, and the list of the research projects are as listed in Appendix 2. The final examination was conducted from the 22 to 29 December 2011. During the examination we were assisted by two external examiners, Dr Dorn Watthanakulpanich, Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Thailand and Associate Professor Dr Zairi Jaal, Vector Control Research Centre, Universiti Sains Malaysia, Penang. Their expert contribution and opinion were invaluable. The examination concluded with fifteen students passing the examination and was awarded the Diploma in Applied Parasitology and Entomology. Two of the fifteen students passed with Distinction, there were Dr. Aye Thinda Win and Ms Nurhainis Ogu Salim. One student was unsuccessful and was awarded a “Certificate of Attendance”. The Top Student Award was awarded to Dr. Aye Thinda Win and she received a prize from the Director of SEAMEO TROPMED Network, Malaysia. The Best Thesis was awarded to Mr. Trevor Desmond Punufimana from Vanuatu. The title of the thesis was “Overlapping of Different Flies From Different Animal Facilities. He received the Deans Best Thesis Award.

4 3. IMR Research Activities

In 2011, staff members of the Institute were engaged in 50 research projects comprising several disciplines including Infectious Tropical Diseases such as dengue, malaria and Chikungunya, Environmental Health, Drug Discovery, Stem Cell, Tuberculosis research, and others. Majority of research projects are sponsored by the Ministry of Health Malaysia, and some by other International and National funding agency. The lsit of research projects conducted in 2011 and 2012 are listed in Appendix 3 and Appendix 4. The Institute published 98 scientific papers in 2011 (Appendix 5) and 43 publications until June 2012 (Appendix 6).

3.1 Allergy and Immunology Research Centre

The research carried out is a continuation of that done in 2010. The Allergy Unit’s research focused on identifying and characterising seafood allergens as well as establishing the basophil activation test. The Autoimmune Disease Unit studied the role of autoantibodies in foetal loss, celiac disease and paraneoplastic syndrome. The unit also commenced research to determine the role of Human Leukocyte Antigen and Anti-Aquaporin 4 antibodies in patients with Multiple Sclerosis. The unit coordinates the National Quality assurance Programme for Antinuclear Antibody Testing (ANA). The Immunogenetics Unit carried out research in the association of disease with major histocompatibility complex. The Primary Immunodeficiency (PID) Unit continued to study and characterise patients and families affected by immunodeficiencies, particularly those with B cell defects, neutrophil dysfunction and Wiskott-Aldrich syndrome. The PID Unit also continued the study on the role of cytokines and other immune parameters in the pathogenesis of dengue.

5 3.2 Cancer Research Centre

The Haematology Unit of the Cancer Research Centre continued to carry out research in haematological malignancies and stem cell. The availability of single nucleotide polymorphism arrays has been applied in hematologic malignancies to detect deletions, amplifications, loss of heterozygosity (LOH) and uniparental disomy at high resolution. The unit have identified genomic imbalances in adult acute lymphoblastic leukaemia (ALL) by genome wide single nucleotide polymorphism analysis. The unit have successfully identified 13 chromosomal aberrations, 24 Copy-neutral LOH (CN-LOH) regions and 23 SNPs in adult ALL. By using array CGH technique, the unit also successfully identified common chromosomal aberrations in multiple myeloma patients. Interestingly, chromosomal aberrations were detected in 100% samples studied. About 90% of these samples were having normal karyotype with conventional cytogenetic. Array CGH can be used as an alternative tool for the identification of copy number variations in myeloma patients in place with conventional cytogenetic. This is especially important if the information is important for patient’s prognostic prediction and treatment decision making. The identification of genomic imbalance has contributed toward the knowledge base of the human genome and lead to a better understanding of the molecular pathogenesis of the haematological cancers.

In stem cell research, the unit studied the multipotency of limbal stromal cells and how these cells differ from mesenchymal and embryonic stem cells in terms of differentiation potentials and gene expression profiles.

The Molecular Pathology Unit of the Cancer Research Centre continued to carry out studies on cancer and genetics, with a particular emphasis on nasopharyngeal carcinoma (NPC), a major cancer in Malaysia.

6 Oral Medicine study on retrospective study of patients seen at the oral medicine clinic, Kuala Lumpur Hospitaly was conducted by the Stomatology research group.

3.3 Cardiovascular, Diabetes & Nutrition Research Centre (CDNRC)

The Nutrition Unit research activities focus on the area of functional food and analytical food chemistry. The current project is on the updates of Malaysian Food Composition Database. The research focuses on analysing new raw and processed foods available in food list. Local Malaysian seaweeds are being studied where selected Malaysian seaweeds were analysed for its nutritional values and identifying functional food properties in seaweeds for in vitro lipase and amylase enzyme inhibitory activities.

3.4 Environmental Health Research Centre (EHRC)

Environmental Health Research Centre continued to conduct research with focus on quality of air, water and assessment of environmental contaminants in food. However research activities had mainly centred on quality of indoor air in hospital premises supplied by Air Handling Units (AHU). This study was using parameters based on ICOP and ASRAE guidelines and standards. The centre provided expertise to other organization in water sampling activities using ultrafiltration methods as well as testing the water sampled for the detection of sarcocystis.

The environmental research group received funding from WHO to conduct research on the development of a risk assessment tool for managing the health impact of climate change. This project is in line with Malaysia’s commitment to addressing the issues of climate change, specifically in protecting health from the impact of global warming due to climate change. At the regional level, Malaysia is committed and contributing to climate change initiative. The project seeks to identify populations most vulnerable to these impacts, develop a functional

7 climate and disease mathematical model for better understanding the relationship between climate change and for forecasting of health outcomes, to assist relevant stakeholders to take steps to assure that these health risks can be managed now and in the future.

3.5 Herbal Medicine Research Centre Research on drug discovery for neglected tropical diseases (Filariasis, malaria and Dengue) are being conducted with compounds obtained from collaborative projects with Drug Companies locally and Internationally. Herbal medicine research focus on natural product discovery from medicinal plant with traditional claims in development of standardized extracts and isolation of compounds for common diseases in Malaysia, including the anti-proliferative activities of the plant extracts to breast cancer and Colorectal Cancer cell lines, anti hypertensive and anti oxidant activity.

Herbal Medicine research group are continuing with the compilation of traditional and scientific uses of local medicinal plants for documentation purposes. The Global Information Hub on Integrated Medicine ‘GlobinMed’ project is ongoing and to present up-to-date and evidence-based information on T&CM (Traditional and Complementary Medicine), by doing it in-house and also working closely with both local and international partners.

The Malaysian Herbal Monograph being one of the EPP (Entry Point Project) of the NKEA (National Key Economic Area) project, embarked on research on preclinical and clinical research for medicinal plant products to provide scientific data for evidence of claims and for regulatory requirements for commercialization.

8 3.6 Infectious Diseases Research Centre (IDRC)

Research in the Acarology Unit continued to focus on 3 main areas: the detection of pathogens in ticks, establishment of a PCR based blood meal identification assay (BMIA), and control of acarine pests of public health importance. The Bacteriology Unit, developed PCR for Bordetella pertussis and Brucella species. An increase in the number of request and positive cases was noted for Bordetella pertussis for the year 2011. The emergence of multidrug-resistance gene NDM-1 among Enterobacteriaceae isolated from patients around the globe has led to the developing of an in-house PCR technique to detect this gene among local isolates. The detection of this gene is crucial for infection control measures and the Bacteriology Unit is the referral laboratory for the confirmation of strains carrying this multi-drug resistant gene. The Medical Entomology Unit continues with the problem-solving research in medical entomology. Research on dengue vectors especially Aedes aegypti is still the main priority for this Unit. The project on transgenic mosquito continued to move forward this year. Other studies on dengue vectors were related to the mosquitocidal effects of various seaweed extracts on the growth and development of Ae. aegypti and Ae. albopictus. With regard to dengue vector surveillance, studies using a modified ovitrap enhanced with hay infusion were found to be effective as an entomological tool for Aedes surveillance. Studies on the effectiveness of thermal fogging for dengue control in closed premises were carried out. In malaria vector research, retrospective assessment was conducted on two long-lasting insecticidal nets (OLYSET® and PERMANET®) after two and four years of household use in Sarawak and Laos respectively. The effectiveness of these nets was tested against laboratory bred Anopheles maculatus. Both nets can be effectively used to prevent mosquito bites even after field used for two years in Sarawak and four years in Laos. Bionomics of the malaria vector An. maculatus in the field was studied and map of larval breeding habitat distribution and malaria transmission risk areas were developed using a combination of field

9 data, satellite image analysis and GIS technique. Studies pertaining to insecticide susceptibility status of An. cracens, vector of Plasmodium knowlesi in Peninsular Malaysia were also conducted. In forensic entomology, study using molecular approach to identify forensically important fly species was successfully completed. A first record of Piophila foveolata larvae (skipping larvae) found on human corpses was reported. DNA profiling for identification of forensically important flies showed that all stages of insects including eggs, egg skin, larvae, pupae, pupa case, adults, and damaged or inappropriately preserved entomological specimens can be employed to identify the species. DNA-based characterisation of selected important forensic Sarcophagid fly in Malaysia was also investigated to facilitate species identification. The Virology Unit focused on research pertaining to locally important medical viruses, aiming to determine the epidemiology of the viruses and the development of new technology for rapid diagnosis of these viruses.

The Virology Unit performed satisfactorily for WHO, RCPA (Australia) and NRL (Australia) Quality Assurance programme for various serological, viral isolation and molecular tests. The National Poliovirus Laboratory received accreditation for Viral Isolation and Identification testing for the 13th successive year and was accredited and entrusted to perform the Vaccine Intratypic Differentiation (ITD) testing and testing for Vaccine-Derived Poliovirus by WHO. Also in 2011, the National Japanese Encephalitis laboratory received full accreditation by WHO.

The programme of HIV sero-surveillance continues with the Virology Unit serving as the National AIDS Reference Laboratory (NARL) for the study of cases with difficult or unresolved serology. The Unit continued to provide consultancy services for babies born to HIV positive women. The Unit also carried out surveillance programme on circulating dengue virus serotype, Nipah, JE, influenza and hand, foot and mouth diseases for Ministry of Health. The dominant circulating dengue serotype for 2011 was Dengue-1 and Dengue-2 and as for

10 influenza, A/Perth/16/2009-like (H3N2) and B/Brisbane/60/2008-like strains were found to be the predominant strains for influenza A and B respectively. This differs from 2010, whereby A/ California/7/2009-like (H1N1) strain was the predominant strain for Influenza A.

3.7 Medical Resource Research Centre (MRRC)

The Medical Research Resource Centre (MRRC) consists was responsible in providing supports in particular on training for officers especially the new medical and research officers in research methodologies, statistical software programmes applied to biomedical research such as SPSS and STATA. The centre was also responsible to conduct several motivation workshops and training on Microsoft Office which included MS Word, Excel, power point and Access and designing website for the general staff of IMR.The centre was also involved in several research projects. They include the effectiveness of pneumococcal vaccine among Malaysian pilgrims performing hajj in Saudi Arabia 2011, the health status of Orang Asli in Peninsular Malaysia and incidence of human plasmodium knowlesi malaria, host-parasite interactions and associated risk factors in Malaysia.

3.8 Specialised Diagnostic Centre focused on research and development towards expanding molecular diagnostics testing with the objective of primarily developing more new molecular genetic testing for the confirmation of selected genetic diseases including IEM. The unit conducted R&D projects for 12 pediatric disorders and the outcome of these are 19 new molecular diagnostic tests for mutational analysis for 17 new genes and two for deletion/duplication of genes.

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4. Diagnostic services.

Being the referral laboratory for the Ministry of Health, the IMR continues to provide and improve clinical laboratory tests. IMR provides specialised and referral diagnostic tests, and tests that are not perform in other laboratories.

The Bacteriology Unit continues to provide diagnostic services to government and private hospitals in Malaysia. The Unit is the referral laboratory for the diagnosis of leptospirosis, pertussis, brucellosis, verification of antibiotic resistance, serotyping and DNA fingerprinting of food and waterborne diseases. It plays an active role in the investigation of nosocomial infections. IMR is Referral Centre for:

The Biochemistry Unit of the Diagnostic Research Centre, has set up a comprehensive screening of urine for inborn error of metabolism using electrospray ionization tandem mass spectrometry.

4.1 IMR is Referral Centre for:

1. WHO Collaborating Centre for the Ecology, Taxonomy and Control of Vectors of Malaria, Filariasis and Dengue for another 4 years. 2. WHO National Influenza Centre, 3. WHO National Laboratory for Poliovirus. In 2011, the laboratory received accreditation for Viral Isolation and Identification testing for the 13th successive year and was accredited and entrusted to perform the Vaccine Intratypic Differentiation (ITD) testing and testing for Vaccine-Derived Poliovirus by WHO. 4. WHO National Japanese Encephalitis Laboratory, It received full accreditation by WHO in 2011, 5. WHO, RCPA (Australia) and NRL (Australia) Quality Assurance program for various serological, viral isolation and molecular tests.

12 6. National AIDS Reference Laboratory (NARL) where programme of HIV sero- surveillance continues for the study of cases with difficult or unresolved serology. 7. Surveillance program on circulating dengue virus serotype, Nipah, JE, influenza and hand, foot and mouth diseases for Ministry of Health Malaysia. The dominant circulating dengue serotype for 2011 was Dengue-1 and Dengue-2 and as for influenza, A/Perth/16/2009-like (H3N2) and B/Brisbane/60/2008-like strains were found to be the predominant strains for influenza A and B respectively. This differs from 2010, whereby A/ California/7/2009-like (H1N1) strain was the predominant strain for Influenza A. 8. Referral laboratory for the diagnosis of leptospirosis, pertussis, brucellosis, verification of antibiotic resistance, serotyping and DNA fingerprinting of food and waterborne diseases. 9. IMR also plays an active role in the investigation of nosocomial infections. 10. IMR coordinates the National Quality assurance Programme for Antinuclear Antibody Testing (ANA). 11. Referral centre for multiple myeloma protein profiling and molecular diagnostic for genetic diseases including Inborn Errors of metabolism (IEM).

5. Consultative services

IMR’s staff provides advisory and consultative services to the Ministry of Health (MOH), other government departments, as well as international organisations. Most units of the Institute also serve as referral centres to MOH laboratories throughout the country. During the year, 86 staff members provided consultative services at the national level, while 18 staff members provided such services at the regional /international level.

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6.Technical Training Programmes, Staff Development and Conferences

6.1 In 2011, 139 staff members of the Institute attended 54 conferences/scientific meetings, whilst 643 staff members attended 372 short courses/workshops to improve their knowledge and skills. There were 17 staff members who had training overseas under the in service training scheme and 97 local training courses were carried out. 34 members of the Institute are currently undergoing post-graduate studies; 14 officers are studying for their Master degrees while 20 are pursuing their PhDs.

6.2 The Institute also conducted 32 training workshops, 17 seminars and 17 courses during the year.

6.3 The regular training courses include the SEAMEO-TROPMED postgraduate courses namely, the Diploma in Applied Parasitology and Entomology and the Diploma in Medical Microbiology courses.

6.4 The Medical Research Resource Centre in collaboration with SEAMEO TROPMED conducted the 9th SEAMEO-IMR Course on Advanced Epidemiology which was held at the Institute for Health Management in Bangsar from 26 September to 7 October 2011. A total of 30 participants attended this course. They were researchers, physicians, statisticians and other health personnel from various countries in South East Asia namely Thailand, The Philippines, Myammar, Vietnam, Cambodia, Lao-PDR and Malaysia. The course was conducted successfully.

6.5 The MRRC was responsible in providing support in particular on training for officers especially the new medical and research officers in research methodologies, statistical software programmes applied to biomedical research

14 such as EpiInfo, SPSS and STATA and few other sub-specialised statistical softwares. The centre was also responsible to conduct motivation workshops and training on Microsoft Office which included MS Word, Excel, power point and Access and designing website for the general staff of IMR. The centre successfully conducted a total of 16 courses throughout 2010.

6.6 The Environmental Health Research Centre (EHRC) conducts the annual Environmental Health Forum with the theme of ‘Air Quality and Impact on Health’ attracted 196 participants to the IMR. Participants were from the Ministry of Health, local universities and other ministry departments such as the Occupational Safety and Health Department, Department of Environment, Department of Chemistry and NIOSH. Speakers were from UKM Bangi, Department of Environment and Institute Respiratory Medicine, Hospital Kuala Lumpur.

6.7 EHRC has continued its role in supporting IMR as a member of the Working Group on Innovation and Research under the National Committee on Green Technology and Climate Change.

6.8 EHRC has also continued successfully to secure and maintain the MS ISO 14001:2004 Standard: Environmental Management System (EMS) certification (since 2005).

6.9 The EHRC with Institute for Health Management (IHM) organised a meeting with WHO experts for the development of a training module for Climate Change and Health for health personnel. Three climate change experts from WPRO, Dr Yasushi Honda, Dr Masahiro Hashizume, and Dr Ho Kim provided technical assistants in the development of the training module specific for health personnel.

15 With reference to Climate change, IMR contributes to the following activities;

 Paper presentation entitled Climate Change Impact on Public Health: Strengthening the Case for Mitigation and Adaptation Strategies in Malaysia at the 22nd PACIFIC SCIENCE CONGRESS subtheme Health Co-Benefits of Climate Change and Green House Gas mitigation from 14-17 JUN 2011 at Kuala Lumpur Convention Centre, Malaysia (Dr. Muhammad Amir Kamaluddin)  Paper presentation entitled Climate Change and Impact on Health at the National Conference on The Impact of Climate Change on Water Resources and their Consequences to Major Economic Sectors on 25 July 2011 organised by Academy of Science Malaysia and National Hydrology Research Institute Malaysia in collaboration with the Ministry of Natural Resources and Environment (Dr. Muhammad Amir Kamaluddin)

 IMR is a member of Working Committee on Research and Innovation under Green Technology and Climate Change Council Malaysia (Dr. Shahnaz Murad, Director of IMR).

 IMR is a member to Working Committee on Adaptation under Green Technology and Climate Change Council Malaysia (Dr. Shahnaz Murad, Director of IMR).

 IMR, as Chair to Expert Group on Health under Research and Innovation Working Committee, within the framework of Green Technology and Climate Change Council Malaysia (Dr. Shahnaz Murad, Director of IMR).

 IMR is a member to Committee for Public Awareness and Promotion within the framework of Green Technology and Climate Change Council Malaysia at MOH level (Dr. Shahnaz Murad, Director of IMR).

16 6.10 The Specialised Diagnostic Centre organised a pre-congress workshop for College of Pathologist titled “Biochemical genetic testing, a laboratory diagnosis for Inborn Error of Metabolism”. The workshop was attended by 90 participants comprising of chemical pathologists, scientists and technologists.

7.International Collaborations  Inter Islamic Network on Tropical Medicine (INTROM),  WHO / TDR Asian Network on Training on Biosafety of Transgenic Insects  Drug for Neglected Diseases Initiatives (DNDi),  Asia Pacific Malaria Elimination Network, Australia (APMEN),  Parasitology Unit, National Institute for Medical Research, Mill Hill, London.  Oxford, Cambridge and Sheffield University, United Kingdom.  CDC Atlanta, National Institute of Health, USA,  Genome Institute, Singapore  Nagasaki and Osaka Univeristy Japan,  Karolinska University, Sweden  NSW University Australia  L V Prasad Eye Institute, Hyderabad, Centre for Research in Entomology, Madurai, India

17 Appendix 1

Topics of Assignment submitted in partial fulfillments of the 2012 DMM Course

No ASSIGNMENT-TITLE STUDENT

1. Preservation and storagae of Microbacteria Mr. Sun Sok San (Cambodia)

2. Primary Immunodificiency Dr. Mohamed Shihan Azeez (Sri Lanka) 3. Reemergence of Bordetella pertussis interaction Mr. Mai Thanh Luan (Vietnam)

4. Quality Control System in diagnostic test for Mrs. UP Nilanthi Perera (Sri viral infection Lanka) 5. Update on bacteria causing causing community Miss Phoutsamay Vongphachanh – acquired pneumonia (Lao PDR) 6. HLA immune responses Mrs Batoul Siddig Mohamed Siddig (Sudan) 7. Autoimmune gastric Diseases Dr. Tin Chn Myat (Myanmar)

8. House Dust mites allergen and immunotherapy Mrs Rafidah Binti Ahmed (Malaysia) 9. Update on bacteria causing community Ms. Surianti bt Shukor (Malaysia) acquired pneumonia 10. Surveillance System involve in managing on of Ms. Pearlisha Bernardine Thomas the important infectious diseases in your (Malaysia) district/state/country 11. Management of water and food borne diseases Ms. Siti Syarinah Bt Abdul Shukor outbreak (Malaysia)

12. Vaccine strategies for the prevention of Mr. Musalman Bin Rambeli common childhood viral diseases (Malaysia)

13. Pheomyscosis in immunodificient patients Dr. Shalini A/P Ponasamy (Malaysia)

18 APPENDIX 2

DAP&E projects thesis submitted in partial fulfillments of the 2011 DAP&E course

No PROJECT-TITLE STUDENT

1. Effectiveness and optimizing of thermal fogging Mrs. Khadijah Khairuddin for dengue control in closed premises. (Malaysia)

2. Effects of the antiviral drug ribavirin on the Mr. Ren Sophorn development of Aedes aegypti larvae (Cambodia)

3. Effectiveness of Cyfluthrin formulation for Aedes Mrs. Noorazlin Mohd Ali pupae control. (Malaysia) 4. Aedes sp. Attaraction towards mixture of Ms.Noor Afizah Ahmad semiochemicals: Semi field evaluation study. (Malaysia) 5. Resurvey of mosquitoes infected with filarial Mr. John Leaburi worms from endemic states of Malaysia using (Solomon Islands) Duplex PCR technique.

6. Optimisation of Multiplex PCR in Diagnosis of Dr. (Mrs) Nounnipha Acanthamoeba and Naegleria spp. Simmalavong (Lao PDR)

7. Study of different malaria sample collection Mr. George Fafale (Solomon methods in the field for DNA yield and detection of Islands) genotypes.

8. Effect of storage of blood samples in Mr. Ken Mera (Vanuatu) anticoagulants in malaria diagnosis.

9. Study on Plasmodium vivax genotype using Mr. Muhammad Nor Farhan merozoite surface protein gene, Pvmsp4 and Saat, (Malaysia) Pvmsp5.

10. Comparative bioefficacy of two long-lasting nets, Mr. Mohd Mazlan Mohd Isa PERMANET® and OLYSET® after field use in (Malaysia) Laos. 11. Mating capacity, sperm production in RDL male Mr. Francis Otto mosquitoes in relation to sizes. (Solomon Islands) 12. Evaluation of Rapid Diagnostic Tests (RDTs) for Mr. Lenny Warele, (Vanuatu) Malaria in detection of Plasmodium knowlesi.

13. Overlapping of different flies from different animal Mr.Trevor Punufimana facilities. (Solomon Islands)

19 14. Larval competition of Aedes aegypti., (L) wild type, Mr. Ho Viet Hieu OX513A-AAE (transgenic strain) and Aedes (Vietnam) albopictus Skuse

15 Development of New Formula For Calculation of Ms. Nurhainis Ogu Salim Malaria Parasites in heavy Parasitemia. (Malaysia)

16. Use of PCR in targeting cytochrome b gene of Dr. Aye thinda Win local animals for blood meal identification. (Myanmar)

20 APPENDIX 3

Research projects conducted in 2011

1. Development of transgenic Aedes mosquitoes

2. Determination of biogenic amines levels in local fish-based food and identifying best manufacturing practices to reduce biogenic amines levels and contaminants

3. Genetic Analysis and Antiviral Resistance Study of Influenza Virus circulating in Malaysia

4. Determination of Biomarkers and Development of Test Kit for Laboratory Diagnosis of Japanese Encephalitis and others Arboviruses

5. Development of DNA Biosensor for the Detection of Tuberculosis

6. Detection of rickettsioses of public health importance in acarine ectoparasites (ticks and mites) using Polymerase Chain Reactions (PCR)

7. Study of the multipotency and global gene expression profile of limbal stromal cells

8. General toxicity study of 5 gamat products: In vivo as well as in vitro toxiclogy studies

9. Human metapneumovirus genotypes among hospitalized patients with upper and lower respiratory tract infection

10. The effect of standardised extracts from local plants [Nephelium lappaceum rind (rambutan) and Syzygium aquem leaves (jambu merah) on Arthritis in animal model

11. Identification of Genomics Aberrations in Adults Acute Lymphoblastic Leukimia (ALL) using High-Density Single Nucleotide Polymorphism Arrays

12. Detection of autoantibodies (anti-gliadin antibodies, anti-endomysium antibodies and anti-tissue transglutaminase antibodies) in diagnosis of coeliac disease

13. Detection of antiphospholipid antibodies in female with fetal loss

14. Detection of neuronal autoantibodies (anti-Hu, anti-Ri, anti-Yo, anti-Ma and amphipysin) in patients suspected with paraneoplastic syndrome (PNS) 15. Development of DNA sequence analysis method (RT-PCR) for identification

21 of nonsporulating fungi and development of new fungal sporulating media

16. Genomic analysis of chromosome aberrations in the pathogenesis of multiple myeloma (MM)

17. Defining B cell defects in Primary Immunodeficiency patients in Malaysia

18. Establishment of a molecular tool acari blood meal indentification in Malaysia

19. Molecular Characterization and Epidemiology of Extended Spectrum Beta- Lactamase producing Escherichia coli, Klebsiella pneumoniae and Klebsilla spp isolates from major Malaysian hospital

20. Sequence Analysis of Dengue Virus in Infected Aedes aegypti Mosquitoes

21. Detection of Biomarkers in Early Dengue Patients for Possible Prediction of Severe Dengue Disease (DHF/DSS)

22. Study of Possible Presence of Virulence Gene of Dengue Virus Associated With Severe Dengue and Emergence of Novel Strain by Sequence Analysis of Dengue Virus Isolates

23. Development of Real-Time PCR Assays for the Detection of H275Y Mutation in Pandemic Influenza A (H1N1) Virus, Season 2009-2010

24. Immune Parameters and Their Role in The Pathogenesis of Dengue and Severe Dengue

25. Effect of Sublethal Dosages of Insecticides on Dengue Virus Replication in Aedes aegypti

26. A DNA Based Approach For The Identification of Forensically Important Fly Species

27. Bionomic studies of Aedes albopictus (Skuse)

28. Identification of Potential Oncogenes and Tumour Suppressor Genes Involved in the Pathogenesis of Oligodendrogliomas and Glioblastomas

29. Optimisation of Cultivation Methods for Isolation of Pathogenic Leptospires and Molecular Characterisation of Malaysian Pathogenic Leptospira Clinical Isolates

30. Rapid Detection of Six Bacterial Pathogens From Cerebrospinal Fluid Specimens of Suspected Meningitis Cases Using Multiplex Real Time PCR 31. Malaria Study in Malaysia

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32. Screening of Compounds for Potential Properties for Neglected Tropical Diseases, Lymphatic Filariasis

33. Molecular Cloning and Characterization of Tropomyosion and Arginine Kinase as Major Allergens of Macrobrachium rosenbergii (Giant Freshwater Prawn)

34. Insecticide Susceptibility Status in Anopheles crescens: Vector of Plasmodium knowlesii in Peninsular Malaysia

35. Association of Single Nucleotide Polymorphisms in the Tumour Necrosis Factor-alpha and Lymphotoxin-alpha (TNF-LTA) Gene Region with Rheumatoid Arthritis

36. Development of In-House HIV-1 Drug Resistance Genotyping Assay and Assessment of the Prevalence of HIV-1 Drug Resistance among HIV Patients.

37. Preclinical Efficacy of MSC Transplantation in an Experimental Model of Liver Cirrhosis

38. Detection and Identification of Pathogenic Agents in Ticks (Acari: Ixodidae)

39. Development of Four Lines Lateral Flow Strip for the Detection of Tuberculosis

40. Acute Toxicity Study of MPOB 7000 (A Base for MPOB GM 1)

41. Biomarkers for Diabetes and Its Associated Complications in Malaysian Adults

42. A Study of Malaysian Families Afftected by a Neutrophill Dysfunction Related Primary Immunodeficiency (PID)

43. A Study of Wiskott-Aldrich Syndrome and Its Related Spectrum of Diseases in Primary Immunodeficiency Patients in Malaysia

44. Updates of Malaysian Food Composition Database

45. Development of New Molecular Detection Methods in Knowlesi Malaria in Malaysia

46. Molecular Characterization of the Simian Malaria Parasite, Plasmodium knowlesi in Malaysia

47. Study of Drug Sensitivity Profile and Genotyping of Plasmodium vivax

23 Isolates in Sabah and Sarawak

48. Incidence of Human Plasmodium knowlesi Malaria, Host Parasite Interaction and Associated Risk Factors in Malaysia

49. A Retrospective Study of Patients with Trigeminal Neuralgia (TGN) Seen at the Oral Medicine Clinic, Kuala Lumpur General Hospital

50. Molecular Characterization of Adenovirus Circulating in Malaysia From 2003 to 2011

51. Translational Oncology Program for the Development of New Assays for Cancers - Screening of Markers for the Diagnosis and/or Prognosis of Nasopharyngeal Carcinoma

52. Identification and Characterization of Cancer Stem Cells in Nasopharyngeal Carcinoma (NPC) Samples

53. The Quantitative Assessment of Chimerism: A Prospective Study in Post Haematopoietic Stem Cell Transplantation Patients

54. In-Vitro Study of Human Adipose Derived Mesenchymal Stem Cell Expressing Tumour Necrosis Factor Related Apoptosis Inducing Ligand or (TRAIL) as a Potential Anti-Tumour Target for Cancer Treatment

55. Comprehensive Analysis for Cancer Predisposition Genes Involved in Adult Acute Lymphoblastic Leukemia Using Multiple Ligation-Dependent Probe Amplification (MLPA)

56. High Resolution Genome-Wide Array Analysis of Acute Lymphoblastic Leukemia (ALL) and Acute Myeloid Leukemia (AML) Based on Malaysian Genetic Profiles

57. Molecular Characterization and Genotyping of Brucella Species Isolated From Cases of Brucellosis

58. The Role of Human Leukocyte Antigen (HLA) Alleles in Susceptibility of Multiple Sclerosis Patients in Malaysia

59. Gene Silencing of Multidrug Resistant Genes in Leukemia Cells

60. Effect of TERT and TERC Gene Expressions in Regulating the Development and Progression of Acute Lymphoblastic Leukemics Cells

61. Establishment of 3D Culture System for Anti-Tumor Spheroid Drug Screening

24 In Vitro Using Brucea javanica Extract as a Model

62. Study of the Effect of Kacip Fatimah dan Misai Kucing Water Extract on Oxidatively Damaged DNA in Colorectal Cancer Cell Line (HCT116 and HT 29) In Vitro

25 APPENDIX 4

Research Projects conducted in 2012

1. Development of transgenic Aedes mosquitoes

2. Determination of biogenic amines levels in local fish-based food and identifying best manufacturing practices to reduce biogenic amines levels and contaminants

3. Determination of Biomarkers and Development of Test Kit for Laboratory Diagnosis of Japanese Encephalitis and others Arboviruses

4. Study of the multipotency and global gene expression profile of limbal stromal cells

5. Genomic analysis of chromosome aberrations in the pathogenesis of multiple myeloma (MM)

6. Molecular Characterization and Epidemiology of Extended Spectrum Beta- Lactamase producing Escherichia coli, Klebsiella pneumoniae and Klebsilla spp isolates from major Malaysian hospital

7. Detection of Biomarkers in Early Dengue Patients for Possible Prediction of Severe Dengue Disease (DHF/DSS)

8. Study of Possible Presence of Virulence Gene of Dengue Virus Associated With Severe Dengue and Emergence of Novel Strain by Sequence Analysis of Dengue Virus Isolates

9. Immune Parameters and Their Role in The Pathogenesis of Dengue and Severe Dengue

10. Effect of Sublethal Dosages of Insecticides on Dengue Virus Replication in Aedes aegypti

11. A DNA Based Approach For The Identification of Forensically Important Fly Species

12. Bionomic studies of Aedes albopictus (Skuse)

13. Identification of Potential Oncogenes and Tumour Suppressor Genes Involved in the Pathogenesis of Oligodendrogliomas and Glioblastomas

14. Optimisation of Cultivation Methods for Isolation of Pathogenic Leptospires

26 and Molecular Characterisation of Malaysian Pathogenic Leptospira Clinical Isolates 15. Rapid Detection of Six Bacterial Pathogens From Cerebrospinal Fluid Specimens of Suspected Meningitis Cases Using Multiplex Real Time PCR

16. Malaria Study in Malaysia

17. Screening of Compounds for Potential Properties for Neglected Tropical Diseases, Lymphatic Filariasis

18. Molecular Cloning and Characterization of Tropomyosion and Arginine Kinase as Major Allergens of Macrobrachium rosenbergii (Giant Freshwater Prawn)

19. Insecticide Susceptibility Status in Anopheles crescens: Vector of Plasmodium knowlesii in Peninsular Malaysia

20. Association of Single Nucleotide Polymorphisms in the Tumour Necrosis Factor-alpha and Lymphotoxin-alpha (TNF-LTA) Gene Region with Rheumatoid Arthritis

21. Development of In-House HIV-1 Drug Resistance Genotyping Assay and Assessment of the Prevalence of HIV-1 Drug Resistance among HIV Patients.

22. Preclinical Efficacy of MSC Transplantation in an Experimental Model of Liver Cirrhosis

23. Detection and Identification of Pathogenic Agents in Ticks (Acari: Ixodidae)

24. Development of Four Lines Lateral Flow Strip for the Detection of Tuberculosis

25. Biomarkers for Diabetes and Its Associated Complications in Malaysian Adults

26. A Study of Malaysian Families Afftected by a Neutrophill Dysfunction Related Primary Immunodeficiency (PID)

27. A Study of Wiskott-Aldrich Syndrome and Its Related Spectrum of Diseases in Primary Immunodeficiency Patients in Malaysia

28. Updates of Malaysian Food Composition Database

29. Development of New Molecular Detection Methods in Knowlesi Malaria in

27 Malaysia

30. Molecular Characterization of the Simian Malaria Parasite, Plasmodium knowlesi in Malaysia

31. Study of Drug Sensitivity Profile and Genotyping of Plasmodium vivax Isolates in Sabah and Sarawak

32. Incidence of Human Plasmodium knowlesi Malaria, Host Parasite Interaction and Associated Risk Factors in Malaysia

33. Molecular Characterization of Adenovirus Circulating in Malaysia From 2003 to 2011

34. Translational Oncology Program for the Development of New Assays for Cancers - Screening of Markers for the Diagnosis and/or Prognosis of Nasopharyngeal Carcinoma

35. Identification and Characterization of Cancer Stem Cells in Nasopharyngeal Carcinoma (NPC) Samples

36. The Quantitative Assessment of Chimerism: A Prospective Study in Post Haematopoietic Stem Cell Transplantation Patients

37. In-Vitro Study of Human Adipose Derived Mesenchymal Stem Cell Expressing Tumour Necrosis Factor Related Apoptosis Inducing Ligand or (TRAIL) as a Potential Anti-Tumour Target for Cancer Treatment

38. Comprehensive Analysis for Cancer Predisposition Genes Involved in Adult Acute Lymphoblastic Leukemia Using Multiple Ligation-Dependent Probe Amplification (MLPA)

39. High Resolution Genome-Wide Array Analysis of Acute Lymphoblastic Leukemia (ALL) and Acute Myeloid Leukemia (AML) Based on Malaysian Genetic Profiles

40. Molecular Characterization and Genotyping of Brucella Species Isolated From Cases of Brucellosis 41. The Role of Human Leukocyte Antigen (HLA) Alleles in Susceptibility of Multiple Sclerosis Patients in Malaysia

42. Gene Silencing of Multidrug Resistant Genes in Leukemia Cells

43. Effect of TERT and TERC Gene Expressions in Regulating the Development and Progression of Acute Lymphoblastic Leukemics Cells

28

44. Establishment of 3D Culture System for Anti-Tumor Spheroid Drug Screening In Vitro Using Brucea javanica Extract as a Model

45. Study of the Effect of Kacip Fatimah dan Misai Kucing Water Extract on Oxidatively Damaged DNA in Colorectal Cancer Cell Line (HCT116 and HT 29) In Vitro

46. Assesment of Risk of Acquiring Iatrogenic Malaria Infection in National Blood Transfusion Service

47. Screening of Wolbachia in Aedes albopictus Skuse, Aedes aegypti (Linnaeus) and Culex sp. in Kuala Lumpur and Selangor

48. Identification of Novel Autoantibodies as Putative Biomarkers in Malaysian Multiple Sclerosis Patients

49. Identification of Biomarkers for Antiphospholipid Syndrome in Females With Fetal Loss in Malaysia

50. Molecular Analysis of Hepatitis B Virus (HBV) Among Hepatitis B Carriers in Malaysia

51. A Study of X-Linked Agammaglobulinemia Patients and Their Family Members in Malaysia

29

APPENDIX 5

PUBLICATION 2011

1 Leong YH, Ismail N, Latiff AA, Nurul Izzah A, Narazah MY and Nurul Ain AB (2011). Nuts consumption pattern among Malaysia adults: a socio- demographic and dietary behaviour perspective. International Food Research Journal 18: 319-328

2 H Muhammad, MR Gomes-Carneiro, KS Poca, ACAX De-Oliveira, A Afzan, SA Sulaiman, Z Ismail and FJR Paumgartten (2011). Evaluation of the genotoxicity of Orthosiphon stamineus aqueous extract. Journal of Ethnopharmacology 133(2): 647-653

3 Lee Fang Chin, Siew Ming Kong, Hoi Ling Seng, Kong Soo Khoo, Rajamurthy Vikneswaran, Siang Guan Teoh, Munirah Ahmad, Soo Beng Alan Khoo, Mohd Jamil Maah and Chew Hee Ng (2011). Synthesis, characterization and biological properties of cobalt (ll) complexes of 1, 10- phenanthroline and maltol. Journal of Inorganic Biochemistry 105(3): 221- 229

4 Rafiza S, Rampal KG and Tahir A (2011). Prevalence and risks factors of latent tuberculosis infection among health care workers in Malaysia. BMC Infectious Diseases 11(1): 19

5 Ahmad N, Zakaria WR and Mohamad R (2011). Analysis of antibiotics susceptibility patterns of Helicobacter pylori isolates from Malaysia. Helicobacter 16(1): 47-51

6 Jeyanthi Suppiah, Mohd Apandi Yusof, Khairul Azuan Othman, TS Saraswathy, Ravindran Thayan, Fauziah Md Kassim, Shahnaz Murad and Zainah Saat (2011). Monitoring of the H275Y mutation in pandemic influenza A (H1N1) 2009 strains isolated in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 42(1): 100-104

7 M Khadri Shahar, A Abu Hassan, HL Lee and MR Che Salmah (2011). Studies on phlebotomine sand fly (Diptera: Psychodidae) poulations in limestone areas and caves of Western Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 42(1): 89-93

30 8 MN Azura, A Norazah, AGM Kamel and S Ahmad Zorin (2011). DNA fingerprinting of septicaemic and localized Burkholderia pseudomallei isolates from Malaysian patients. Southeast Asian Journal of Tropical Medicine and Public Health 42(1): 114-121

9 G Virnomalini, Tg Rogayah, TS Saraswathy, R Thayan, M Apandi, MK Fauziah and Zainah Saat (2011). Molecular typing of dengue viruses circulating in East Coast of Peninsular Malaysia from 2005 to 2009. Southeast Asian Journal of Tropical Medicine and Public Health 42(1): 114-121

10 Choong-Chor Chang, Chun-Lai Too, Shahnaz Murad and Suraiya Hani Hussein (2011). Association of HLA-B* 1502 allele with carbamazepine- induced toxic epidermal necrolysis and Steven-Johnson syndrome in multi-ethnic Malaysian population. International Journal of Dermatology 50(2): 221-224

11 Maizatul Hasyima Omar, William Mullen and Alan Crozier (2011). Identification of proanthocyanidin dimers and trimers, flavone C- glyciosides, and antioxidants in Ficus deltoidea, a Malaysian herbal tea. Journal of Agriculture and Food Chemistry 59: 1363-1369

12 Yan Pan, Badrul Amini Abd-Rashid, Zakiah Ismail, Rusli Ismail, Joon Wah Mak, Peter CK Pook, Hui Meng Er and Chin Eng Ong (2011). In vitro modulatory effects of Andrographis paniculata, Centella asiatica and Orthosiphon stamineus on cytochrome P450 2C19 (CYP2C19). Journal of Ethnopharmacology 133(2): 881-887

13 Mohd Apandi Yusof, Fazilah Rais, Maizatul Akma Abdullah, Liyana Ahmad Zamri, Hariyati Md Ali, Fauziah Md Kasim and Zainah Saat (2011). Molecular epidemiology of human enterovirus71 (HEV71) strains isolated in Peninsular Malaysia and Sabah from year 2001 to 2009. Journal of General and Molecular Virology 3(1): 18-26

14 Lim KH, Sumarni MG, Lim KK, Tee EO, Christopher VM, Noruiza Hana M and Amal NM (2011). Do adolescents overestimate the prevalence of smoking among their peers? Finding from a study in Petaling District, Selangor, Malaysia. Malaysian Journal of Public Health Medicine 11(2): 6- 12

15 Wan Nazaimoon Wan Mohamud, Kamarul Imran Musa, Amir Sharifudin Mohd Khir, Aziz Al Safi Ismail, Ikram Shah Ismail, Khalid Abd Kadir, Nor Azmi Kamaruddin, Nor Azwany Yacob, Norlaila Mustafa, Osman Ali, Siti Harnida Mohd Isa, Wan Mohamad Wan Bebakar (2011). Prevalence of overweight and obesity among adult Malaysia: an update. Asia Pacific Journal of Clinical Nutrition 20(1): 35-41

31 16 Florence LCH, Hakim SL, Kamaludin MA and Thong KL (2011). Determination of toxinotypes of enviromental Clostridium perfringens by Polymerase Chain Reaction. Tropical Biomedicine 28(1): 171-174

17 Zailatul Hani Mohamad Yadzir, Rosmilah Misnan, Normalin Abdullah, Masita Arip and Shahnaz Murad (2011). Identification of major allergens of wildflower honey. Southeast Asian Journal of Tropical Medicine and Public Health 42(2): 370-375

18 TS Saraswathy, A Az-Ulhusna, R Nurul Ahikin, S Suriani and S Zainah (2011). Sero-prevalence of cytomegalovirus infections in pregnant women and associated role in obstetric complications: A preliminary study. Southeast Asian Journal of Tropical Medicine and Public Health 42(2): 320-322

19 GH Tee, P Ramanathan, NM Amal and K Chinna (2011). Health seeking behaviour among Malaysian with acute diarrheal disease. Southeast Asian Journal of Tropical Medicine and Public Health 42(2): 424-435

20 Wan Najdah Wan Mohamad Ali, Rohani Ahmad, Zurainee Mohd Nor, Zamree Ismail and Lee Han Lim (2011). Population dynamics of adults mosquitos (Diptera: Culicidae) in malaria endemic villages of Kuala Lipis, Pahang Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 42(2): 259-267

21 K Gurpreet, GH Tee, NM Amal, R Paramesarvathy and C Karuthan (2011). Incidence and determinants of acute diarrhoea in Malaysia: A population -based Study. Journal of Health, Populotion and Nutrition 29(2): 1-10

22 NM Amal, R Paramesarvathy, GH Tee, K Gurpreet, C Karuthan (2011). Prevelance of chronic illness and health seeking behaviour in Malaysian population: Results from The Third National Health Morbidity Survey (NHMS III) 2006. The Medical Journal of Malaysia 66(1): 41-46

23 Norzahira R, Hidayatulfathi O, Wong HM, Cheryl A, Firdaus R, Chew HS, Lim KW, Sing KW, Mahathavan M, Nazni WA, Lee HL, Vasan SS, Mc Kemey A and Lacroix R (2011). Ovitrap surveillance of the dengue vectors, Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus Skuse in selected areas in Bentong, Pahang, Malaysia. Tropical Biomedicine 28(1): 48-54

24 Mariana A, Mohd Khulaimi B, Halimaton I, Suhaili ZA, Shahrul-Anuar MS, Nor Zalipah M and Ho TM (2011). Acarine ectoparasites of Panti Forest Reserve in Johore, Malaysia. Asian Pacific Journal of Tropical Biomedicine 1(1): 1-5

32

25 AY Faudzi, NM Amal, AO Zainal, I Lailanor, H Sirajuddin and MA Taha (2011). Improvement in medically certified death: results from a pilot study in the State of Malacca, Malaysia. Medical Journal of Malaysia 66(1): 26- 29

26 TS Saraswathy, AS Santana Raj, Khairul A Othman, Aida Suhana R, Izan Izan Shahrina A and Zainah S (2011). Apoptosis versus necrosis: electron microscopy observations of cell death events in influenza A virus and chikungunya virus induced infection of cultured cells. Annals of Microscopy 11: 44-49

27 Mariana A, Vellayan S, Halimaton I and Ho TM (2011). Acariasis on pet Burmese python, Python molurus bivittatus in Malayia. Asian Pacific Journal of Tropical Medicine 4(3): 227-228

28 Azima Laili Hanifah, Siti Hazar Awang, Ho Tze Ming, Suhaili Zainal Abidin and Maizatul Hashima Omar (2011). Acaricidal activity of Cymbopogon citratus and Azadirachta indica against house dust mites. Asian Pacific Journal of Tropical Biomedicine 1(5): 365-369

29 AR Kerr, S Warnakulasuriya, AJ Mighell, T Dietrich, M Nasser, J Rimal, A Jalil, MM Bornstein, T Nagao, F Fortune, VH Hazarey, PA Reichart, S Silverman and NW Johnson (2011). A systematic review of medical interventions for oral submucous fibrosis and future research opportunities. Oral Diseases 17(suppl. 1): 42-57

30 Yih-Yih Kok, Wan-Loy Chu, Siew-Moi Phang, Shar Mariam Mohamed, Rakesh Naidu, Pey-Jiun Lai, Shui-Nyuk Ling, Joon-Wah Mak, Patricia Kim-Chooi Lim, Pauline Balraj and Alan Soo-Beng Khoo (2011). Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells. Journal of Zhejiang University Science B 12(5): 335-345

31 Murali Krishna Mamidi, Rajarshi Pal, Nor Azah binti Mori, Greetha Arumugam, Saratha Thevi Thrichelvam, Puteri J Noor, Hj Mohamad Farouk Abdullah, Pawan Kumar Gupta, Anjan Kumar Das, Zubaidah Zakaria and Ramesh Bhonde (2011). Co-culture of mesenchymal-like stromal cells derived from human foreskin permits long term propagation and diffentiation of human embryonic stem cells. Journal of Cellular Biochemistry 112(5): 1353-1363

32 Nadarajan VS, Phan CL, Ang CH, Liang KL, Gan GG, Bee PC and Zakaria Z (2011). Identification of copy number alterations by array comparative genomic hybrodization in patients with late chronic or

33 accelerated phase chronic myeloid leukemia treated with imatinib mesylate. International Journal of Hematology 93(4): 465-473

33 Mamidi MK, Pal R, Govindasamy V, Zakaria Z and Bhonde R (2011). Treat the graft to improve the regenerative ability of the host. Medical Hypotheses 76(4): 599-601

34 CC Kee, K H Lim, MG Sumarni, MN Ismail, BK Poh and NM Amal (2011). Physical activities and sedentary behaviour among adolescents in Petaling District, Selangor, Malaysia. Malaysian Journal of Medicine and Health Sciences 7(1): 83-93

35 AR Ghazali, R Abdullah, N Ramli, NF Rejab, MS Ahmad-Kamal and NA Yahya (2011). Mutagenic and antimutagenic activities of Mitragyna speciosa Korth extract using Ames test. Journal of Medicinal Plants Research 5(8): 1345-1348

36 Ibrahim Jantan, Nurul Hikmah Harun, Abdi Wira Septama, Shahnaz Murad and MA Mesaik (2011). Inhibition of chemiluminescence and chemotatic activity of phagocytes in vitro by extracts of selected medicinal plants. Journal of Natural Medicines 65(2): 400-405

37 Yan Pan, Badrul Amini Abd-Rashid, Zakiah Ismail, Rusli Ismail, Joon Wah Mak, Peter CK Pook, Hui Meng Er and Chin Eng Ong (2011). In vitro of active constituents and extracts of Orthosiphon stamineus on the activities of three major human cDNA-expressed cytochrome P450 enzymes. Chemico-Biological Interactions 190(1): 1-8

38 Siu Kuin Wong, Yau Yan Lim, Noor Rain Abdullah and Fariza Juliana Nordin (2011). Assessment of antiproliferative and antiplasmodial activities of five selected Apocynaceae species. BMC Complementary and Alternative Medicine 11:3

39 Mohd Apandi Yusof, Lau Sau Kuen, Norfaezah Adnan, Nur Izmawati Abd Razak, Liyana Ahmad Zamri, Khairul Izwan Hulaimi and Zainah Saat (2011). Epidemiology and molecular characterization of chikungunya virus involved in the 2008 to 2009 outbreak in Malaysia. Journal of General and Molecular Virology 3(2): 35-42

40 Norlaila Mustafa, Nor Azmi Kamarudin, Ab Aziz Ismail, Amir Sharifuddin Khir, Ikram Shah Ismail, Kamarul Imran Musa, Khalid Abdul Kadir, Nor Azwany Yaacob, Osman Ali, Siti Harnida Md Isa, Wan Mohamad Wan Bebakar and Wan Nazaimoon Wan Mohamud (2011). Prevalence of abnormal glucose tolerence and risk factors in urban and rural Malaysia. Diabetes Care 34: 1362-1364

34 41 SZ Halim, NR Abdullah, A Afzan, BA Abdul Rashid, I Jantan and Z Ismail (2011). Study of acute toxicity of Carica papaya leaf extracts in Sprague Dawley rats. Journal of Medicinal Plant Research 5(10): 1867- 1872

42 Saliza Anida Salleh, Nor Fadilah Rejab, Noor Rain Abdullah, Zakiah Ismail, Peter Mouatt, Ashley Dowell and Shamsul Muhamad (2011). In vitro chemopreventive activity of an ethyl acetate fraction derived from hot water extract of Orthosiphon stamineus in HepG2 cells. Journal of Medicinal Plant Research 5(10): 1892-1899

43 Fauziah Md Kassim, M Nur Izati, TAR TgRogayah, Y Mohd Apandi and Zainah Saat (2011). Use of dengue NS1 antigen for early diagnosis of dengue virus infection. Southeast Asian Journal of Tropical Biomedicine and Public Health 42(3): 562-569

44 Mashitah M. Yusoff, Halijah Ibrahim and Nurulhusna A. Hamid (2011). Chemical characterization and antimicrobial activity essential oils of very closely allied Zingiberaceae species endemic to Borneo: Alpinia ligulata K. Schum. and Alpinia nieuwenhuizii VAL. Chemistry and Biodiversity 8(5): 916-923

45 Aswir Abd Rashed (2011). In vitro study to determine the effect of zinc on non-heme iron absorption. International Journal of Collaborative Research on Internal Medicine and Public Health 3(5): 354-368

46 Aswir AR and Wan Nazaimoon WM (2011). Effect of edible bird's nest on cell proliferation and tumour necrosis factor alpha (TNF-a) release in vitro. International Food Research Journal 18(3): 1073-1077

47 Mansor Fazliana, Nubia L Ramos, Petra Luthje, Musa Sekikubo, Asa Holm, WM Wan Nazaimoon and Annelie Brauner (2011). Labisia pumila var. alata reduces bacterial load by inducing uroepithelial cell apoptosis. Journal of Ethnopharmacology 136(1): 111-116

48 Too Chun Lai, Leonid Padyukov, Jasbir Singh Dhaliwal, Emeli Lundstrom, Abqariyah Yahya, Nor Asiah Muhamad, Lars Klareskog, Lars Alfredsson, Per Tobias Larsson, Shahnaz Murad and MyEira Group (2011). Shared epitope alleles remain a risk factor for anti-citrullinated proteins antibody (ACPA) - positive rheumatoid arthritis in three asian ethnic groups. PLoS One 6(6): e21069

49 H Rozilawati, AY Faudzi, AA Siti Rahidah, AH Nor Azlina, AG Abdullah, NM Amal, H Wan Mansor, H Hani, Y Apandi, Faezah Noor, Norziyana, WA Nazni, J Zairi and HL Lee (2011). Entomological study of chikungunya infectious in the State of Kelantan, Malaysia. Indian Journal

35 of Medical Research 133: 670-673

50 Yan Pan, Badrul Amini Abd Rashid, Zakiah Ismail, Rusli Ismail, Joon Wah Mak, Peter CK Pook, Hui Meng Er and Chin Eng Ong (2011). In vitro determination of the effect of Andrographis paniculata extracts and andrographolide on human hepatic cytochrome P450 activities. Journal of Natural Medicine 65(3-4): 440-447

51 Uma Devi Palanisamy, Muniswaran Sivanathan, Ammu Kutty Radhakrishnan, Nagaraja Haleagrahara, Thavamanithevi Subramaniam and Gan Seng Chiew (2011). An effective ostrich oli bleaching technique using peroxide value as an indicator. Molecules 16: 5709-5719

52 Syuhaidah Sahabudin, Rosmilah Misnan, Zailatul Hani Mohamad Yadzir, Jamaludin Mohamad, Noormalin Abdullah, Faizal Bakhtiar and Shahnaz Murad (2011). Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus). Malaysian Journal of Medical Science 18(3): 27-32

53 A Madinah, A Fatimah, A Mariana and MT Abdullah (2011). Ectoparasites of small mammals in four localities of wildlife reserves in Peninsular Malaysia. Southeast Asian Journal of Tropical Biomedicine and Public Health 42(4): 803-813

54 Siu Kuin Wong, Yau Yan Lim, Noor Rain Abdullah and Fariza Juliana Nordin (2011). Antiproliferative and phytochemical analyses of leaf extracts of ten Apocynaceae species. Pharmacognosy Research 3(2): 100-106

55 Rohani Md Yasin, Norni Mohd Zin, Azura Hussin, Salbiah Haji Nawi, Suhailah Md Hanapiah, Zubaidah Abdul Wahab, Ganeswarie Raj, Norazita Shafie, Ng Pey Peng, Keah K Chui, Muhhamad Nazri Aziz, Nurahan Maning, Jamilah Said Mohamad, Adom Benjamin, Mohd. Azmi bin Mohd. Salleh, Sabarina Sabihah Zahari, Alex Francis, Norazah Ahmad and Rina Karunakaran (2011). Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals. Vaccine 29(34): 5688-5693

56 Mohd Fuat Abdul Razak and Kofi E Aidoo (2011). Toxicity studies of Eurycoma longifolia (Jack)-based remedial products. Asian Journal of Pharmaceutical and Clinical Research 4(3): 23-27

57 Yew Keong Choong, Su-Qin Sun, Qun Zhou, Zakiah Ismail, Badrul Amini Abdul Rashid and Jia-Xun Tao (2011). Determination of storage stability of the crude extracts of Ganoderma lucidum using FTIR and 2-D-

36 IR spectroscopy. Vibrational Spectroscopy 57(1): 87-96

58 Fairuz Amran, M Nazri Aziz, Hishamshah M Ibrahim, NH Atiqah, S Parameswari, MR Hafiza and M Ifwat (2011). Invitro antifungal susceptibilities of Candida isolates from patients with invasive candidiasis in Kuala Lumpur Hospital, Malaysia. Journal of Medical Microbiology 60(9): 1312-1316

59 Rohani A, Suzilah I, Malinda M, Anuar I, Mohd Mazlan I, Salmah Maszaitun M, Topek O, Tanrang Y, Ooi SC, Rozilawati H and Lee HL (2011). Aedes larval population dynamics and risk for dengue epidemics in Malaysia. Tropical Biomedicine 28(2): 237-248

60 Mohd Fairulnizal Md Noh and Ibtisam E Tothill (2011). Determination of lead (II), cadmium (II) and cooper (II) in waste-water and soil extracts on mercury film screen-printed carbon electrode sensor. Sains Malaysiana 40(10): 1153-1163

61 YN Azwany, Wan Mohamad Wan Bebakar, Kamarul Imran M, Aziz al-Safi Ismail, Amir Sharifuddin Md Khir, Ikram Shah Ismail, Khalid Abdul Kadir, Nor Azmi Kamaruddin, Norlaila Mustafa, osman Ali, Siti Harnida Md Isa and Wan Nazaimoon WM (2011). Clustering of metabolic syndrome factors in Malaysian population: Asian criteria revisited. International Journal of Collaborative Research on Internal Medicine and Public Health 3(8): 655-664

62 Yin-Hui Leong, Ahmad Rosma, Aishah A Latiff and Nurul Izzah Ahmad (2011). Exposure assessment and risk characterization of aflatoxin B1 in Malaysia. Mycotoxin Research 27(3): 207-214

63 Husniza Hussain, Mohd Fuat AR, Vimala B and Ghazali HM (2011). Screening method for detection of immediate amino acid decarboxylase - producing bacteria implicated in food poisoning. Tropical Biomedicine 28(2): 351-361

64 Ahamad M, Louis SR, Hamid Z and Ho TM (2011). Scanning electron micrographs of medically important dust mite, Suidasia pontifica (Acari: Astigmata: Saproglyphidae) in Malaysia. Tropical Biomedicine 28(2): 275- 283

65 Normaznah Yahaya, Saniah Kaspan and Noor Rain Abdullah (2011). Immunoblot analysis of Legionella pneumophila serogroup 1 antigens using polyclonal and monoclonal antibodies. International Medical Journal 18(3): 217-219

37 66 Yeong YS, Nazni WA, Santhana RL, Mohd Noor I, Lee HL and Mohd Sofian A (2011). Scanning electron microscopic evaluation of the successful sterilization of Lucilia cuprina (Wiedemann) utilized in Maggot Debridement Therapy (MDT). Tropical Biomedicine 28(2): 325-332

67 Hidayati H, Nazni WA, Lee HL and Sofian-Azirun M (2011). Insecticide resistence development in Aedes aegypti upon selection pressure with malathion. Tropical Biomedicine 28(2): 425-437

68 Tang JC, Wong SF, Mak JW and Ho TM (2011). Antigenic profile of Blomia tropicalis, Aleuroglyphus ovatus and Glycycometus malaysiensis. Tropical Biomedicine 28(2): 223-236

69 Jama'ayah MZ, Heu JY and Norazah A (2011). Seroprevalance of brucellosis among suspected cases in Malaysia. Malaysian Journal of Pathology 33(1): 31-34

70 Pauline Balraj, Huraizah Sidek, Jeyanthi Suppiah, Alan Soo Beng Khoo and Zainah Saat (2011). Molecular analysis of 2009 pandemic Influenza A (H1N1) in Malaysia associated with mild and severe infections. Malaysian Journal of Pathology 33(1): 7-12

71 Nazni WA, Jeffery J, Lee HL, Lailatul Akmar MN, Chew WK, Heo CC, Sadiyah I, Khairul Asuad M, Heah SK and Mohd Hisham H (2011). Nosocomial nasal myiasis in intensive care unit. Malaysian Journal of Pathology 33(1): 53-56

72 Adiratna Mat Ripen, Takeshi Nitta, Shigeo Murata, Keiji Tanaka and Yousuke Takahama (2011). Ontogeny of thymic cortical epithelial cells expressing the thymoproteasome subunit B5t. European Journal of Immunology 41(5): 1278-1287

73 Dhaliwal JS, Wong L, Kamaluddin MA, Yin LY and Murad S (2011). Susceptibility to aplastic anemia is associated with HLA-DRB1*1501 in an aboriginal population in Sabah, Malaysia. Human Immunology 72(10): 889-892

74 Nazni Wasi Ahmad, Lee Han Lim, Chen Chee Dhang, Hoe Chong Chin, Abdullah AG, Wan-Norjuliana Wan Mustaffa, Chew Wai kian, John Jeffery, Rosli Hashim and Sofian Mohd Azirun (2011). Comparative insect fauna succession on indoor and outdoor monkey carrions in a semi- forested area in Malaysia. Asian Pacific Journal of Tropical Biomedicine : S231- S237

38 75 Emilia Zainal Abidin, Sean Semple, Affandi Omar, Hejar A Rahman, Stephen W Turner and Jon G Ayres (2011). A survey of schoolchildren's exposure to secondhand smoke in Malaysia. BMC Public Health 11: 634- 644

76 Shew Fung Wong, Ai Ling Chong, Joon Wah Mak, Jessie Tan, Suk Jiun Ling and Tze Ming Ho (2011). Molecular identification of house dust mites and storage mites. Experimental and Applied Acarology 55(2): 123-133

77 Noorhaida Ujang, Kamaluddin A, Kamaluddin F, Syed Sheikh SH, CM Din SNA, Ariffin A and Sulaiman A (2011). Cholera outbreak in Village A, Tanjung Keling, Melaka, 2007. OSIR 4(1): 12-19

78 DN Pengiran Suhaili, S Somasundaram, SH Lau, AJ Ajura, AR Roslan and R Ramli (2011). Duplication of lower lip and mandible - a rare diprosopus. International Journal of Pediatric Otorhinolaryngology 75(1): 131-133

79 R Issa, NA Hamdan, ASS Raj and MFM Noh (2011). Physical characterization of the screen-printed carbon electrode surface using scanning electron micrograph. ASM Science Journal 5(1): 36-42

80 Bushra Afroze and Zabedah Md Younus (2011). Iatrogenic severe protein deficiency in a child. Journal of The College of Physicians and Surgeons Pakistan 21(11): 718-720

81 GH Shahraki, MN Hafidzi, MS Khadri, J Rafinejad and YB Ibrahim (2011). Cost-effectiveness of integrated pest management compared with insecticidal spraying against the German Cockroach in apartment building. Neotropical Entomology 40(5): 607-612

82 Lim KH, Sumarni MG, Kee CC, Amal NM and Norhamimah A (2011). Susceptibility to smoking as a predictor of smoking initiation among adolescents - a longitudinal study in Kota Tinggi District, Malaysia. Medicine and Health 6(1): 49-58

83 Nor Rizan K, Abdul Manaf A, Sabariah AR, Siti Aishah MA, Noorjahan Banu MA and Zubaidah Z (2011). Sybr green real-time PCR is a useful method in screeining for high risk human papilloma virus types 16 and 18 in neoplastic cervical cancer. Medicine and Health 6(1): 59-67

84 Wan Norafikah O, Nazni WA, Noramiza S, Shafa'ar Ko'ohar S, Heah SK, Nor Azlina AH, Abdullah AG, Sa'diyah I, Khairul-Asuad M and Lee HL (2011). Ovitrap surveillance and mixed infestation of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in nothern region and southern

39 region of Malaysia. Health and Environment Journal 2(1): 1-5

85 Yan Pan, Badrul Amini Abd-Rashid, Zakiah Ismail, Rusli Ismail, Joon Wah Mak and Chin Eng Ong (2011). Heterologous expression of human cytochromes P450 2D6 and CYP3A4 in Escherichia coli and their functional characterization. Protein Journal 30: 581-591

86 Faradianna E Lokman, Norhashimah A Seman, Aziz al-Safi Ismail, Norazwany Yaacob, Norlaila Mustafa, Amir SM Khir, Zanariah Hussien and Wan Nazaimoon Wan Mohamud (2011). Gene expression profiling in ethnics Malays with type 2 diabetes mellitus, with and without diabetes nepropathy. Journal of Nephrology 24(6): 778-789

87 Nor Azrina Norahmad, Noor Rain Abdullah, Norhayati Yaccob, Jenarun Jelip, Jiloris F Dony, Khairul Faiz Ruslan, Lokman Hakim Sulaiman, Hasidah Mohd Sidek, Harald Noedl and Zakiah Ismail (2011). High prevalence of PFCRT K77T mutants among Plasmodium falciparum isolates from Sabah, Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 42(6): 1322-1326 88 MN Lim, T Umapathy, PJN Baharuddin, and Z Zubaidah (2011). Characterization and safety assessment of bioengineered limbal epithelium. The Medical Journal of Malaysia 66(4): 335-341

89 Chung DR, Song JH, Kim SH, Thamlikitkul V, Huang SG, Wang H, So TM, Yasin RM, Hsueh PR, Carlos CC, Hsu LY, Buntaran L, Lalitha MK, Kim MJ, Choi JY, Kim SI, Ko KS, Kang CI, Peck KR, and on behalf of the ANSORP Study Group (2011).High prevalence of multidrug-resistant nonfermenters in hospital-acquired pneumonia in Asia. American Journal of Respiratory and Critical Care Medicine 184(2): 1409-1417

90 Roslina Husaini, Munirah Ahmad and Alan Soo-Beng Khoo (2011). Epstein-Barr virus latent membrane protein LMP1 reduce p53 protein levels independent of the P13K - Akt pathway. BMC Research Notes 4:551

91 Rohani Ahmad, Wan Najdah Wan Mohamad Ali, Zurainee Mohamad Nor, Zamree Ismail, Azhari Abdul Hadi, Mohd Noor Ibrahim and Lee Han Lim (2011). Mapping of mosquito breeding sites in malaria endemic areas in Pos Lejang, Kuala Lipis, Pahang, Malaysia. Malaria Journal 10:361

92 CC Kee, H Jamaiyah, A Geeta, Z Ahmad Ali, MN Noor Safiza, S Suzana, GL Khor, R Rahmah, AR Jamalludin, MG Sumarni, KH Lim, Y Ahmad Faudzi and NM Amal (2011). Sensitivity and spesificity of waist circumference as a single screening tool for identification of overweight and obesity among Malaysian adults. Medical Journal of Malaysia 66(5): 462-467

40

93 Ivyna Bong PN, Zubaidah Z, Rohaizak M, Naqiyah I, Nor Aina E, Noor Hisham A and Sharifah NA (2011). Elevated expression of CD151 gene in estrogen receptor and progesterone receptor positive breast carcinoma. Medicine & Health 6(1): 33-40

94 B Vimala, H Husniza and WM Wan Nazaimoon (2011). Effect of edible bird's nest on tumour necrosis factor alpha secretion, nitric oxide production and cell viability of lipopolysaccharide-stimulated RAW 264.7 macrophages. Food and Agricultural Immunology pp 1-12

95 Sima Ataollahi Eshkoor, Patimah Ismail, Sabariah Abd Rahman, Saidi Moin and Mohd Yusoff Adon (2011). Occupational exposure as a risk factor to enhance the risk of early ageing. Asian Journal of Biotechnology 3(6): 573-580 96 Tan Geok Wee, Tan Lu Ping and Alan Khoo Soo Beng (2011). Review: Lab-based tests for nasopharyngeal carcinoma. Bulletin National Committee for Clinical Research 1: 17-21 97 S Jameela, SO Sharifah Sabirah, CL Phan, KM Chang, MA Salwana, A Zuraidah, Y Subramaniam and A Rahimah (2011). Thalassaemia screening among students in a secondary school in Ampang, Selangor. Medical Journal of Malaysia 66(5): 522-524 98 AJ Ajura and SH Lau (2011). Lymphoma of the orofacial region diagnosed by Stomatology Unit, Institute for Medical Research (2000 - 2010). Oral Oncology 47: S127-S128

41 APPENDIX 6

PUBLICATION 2012

1 Zailatul Hani Mohamad Yadzir, Rosmilah Misnan, Noormalin Abdullah, Faizal Bakhtiar, Masita Arip and Shahnaz Murad (2012). Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn). Asian Pacific Journal of Tropical Biomedicine 2(1): 50-54

2 Ernieenor Faraliana Che Lah, Mariana Ahamad, Mohd Subail Haron and Ho Tze Ming (2012). Establishment of a molecular tool for blood meal identification in Malaysia. Asian Pacific Journal of Tropical Biomedicine 2(3): 223-227

3 Suhaili Zainal Abidin and Ho Tze Ming (2012). Effect of a commercial air ionizer on dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acari: Pyroglyphidae) in the laboratory. Asian Pacific Journal of Tropical Biomedicine 2(2): 156-158

4 Rahizan I, Nurul Akma MH, Hatijah A, Siti Hasmah H, Valentinus HS and Athirah AS (2012). Detection and discrimination of Mycobacterium tuberculosis complex. Diagnostic Microbiology and Infectious Disease 72(1): 62-67

5 Paramasvaran S, Sani RA, Krishnasamy M, Amal Nasir M, Hassan L, Mohd Zain SN, Jeffery J, Santhana Raj L, Latifa I, Abdul Aziz, Vellayan S and Selvanesan S (2012). An overview of nematodes infecting urban and wild rats (Muridae) in Malaysia. Malaysian Journal of Veterinary Research 3(1): 15-21

6 Low SY, Tan BS, Choo HL, Tiong KH, Khoo AS and Leong CO (2012). Suppression of BCL-2 synergizes cisplatin sensitivity in nasopharyngeal carcinoma cells. Cancer Letter 314(2): 166-175

7 Zailatul Hani Mohamad Yadzir, Rosmilah Misnan and Shahnaz Murad (2012). Identification of tropomyosin as major allergen of white squid (Loligo edulis) by two-dimensional immunoblotting and mass spectrometry. Southeast Asian Journal of Tropical Medicine and Public Health 43(1): 185-191

8 Florence CH Lee, SL Hakim, MA Kamaluddin and Kwai Lin Thong (2012). Clostridium perfringens and sulphite reducing Clostridia densities in selected tropical Malaysian rivers. Southeast Asian Journal of Tropical Medicine and Public Health 43(1): 129-135

42 9 Loke Seau Rong, Andy Tan Wei Ann, Nazni Wasi Ahmad, Lee Han Lim and Mohd Sofian Azirun (2012). Insecticide susceptibility status of field- collected Aedes (Stegomyia) aegypti (L.) at a dengue endemic site in Shah Alam, Selangor, Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 43(1): 34-47 10 Sudha Balasubramaniam, Parvathy Venugopal, Swathi Sundarraj, Zubaidah Zakaria, Anish Sen Majumdar and Malancha Ta (2012). Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells. Cytotheraphy 14(1): 26-33 11 S Rafiza and KG Rampal (2012). Serial testing of Malaysian health care workers with QuantiFERON-TB Gold In-Tube. The International Journal of Tuberculosis and Lung Disease 16(2): 163-168

12 Ami F Mohamed, Elisabet I Nielsen, Otto Cars and Lena E Friberg (2012). Pharmacokinetic-pharmacodynamic model for gentamicin and its adaptive resistance with predictions of dosing schedules in newborn infants. Antimicrobial Agents and Chemotheraphy 56(1): 179-188

13 Noor Azuwa Hamdan, Rahizan Issa, Mohd Fairulnizal Md Noh and Noraziah Mohamad Zin (2012). Electrochemical technique using Methylene Blue with Pencil Graphite Electrode for optimum detection of Mycobacterium tuberculosis DNA. Current Research in Tubercolusis 4(1): 1-12

14 Z Md Yunus, DP Abg Kamaludin, M Mamat, YS Chong and LH Ngu (2012). Biochemical profiles of maple syrup urine disease in Malaysian children. JIMD Reports 5(1): 99-107

15 S Balasubramaniam, YS Chong, A Talib, MD Norsiah, LP van den Heuvel and RJ Rodenburg (2012). Infantile progressive hepatoencephalomyopathy with combined OXPHOS deficiency due to mutations in the mitochondrial translation elongation factor gene GFMI. JIMD Reports 5(1): 113-122

16 Chong Huat Siar, Shin Hin Lau and Kok Han Ng (2012). Ameloblastoma of the Jaws: a retrospective analysis of 340 cases in a Malaysian population. Journal of Oral and Maxillofacial Surgey 70(3): 608-615

17 Ratna Mohd Tap, Parameswari Sabaratnam, Mohd Azmi Mohd Salleh, Mohd Fuat Abd Razak and Norazah Ahmad (2012). Characterization of Protorheca wickerhamii isolated from disseminated algaemia of kidney transplant patient from Malaysia. Mycopathologia 173(2-3): 173-178

43 18 Mohd Fadly Md Ahid, Azli Ismail, Thong Meow Keong, Narazah Mohd Yusoff and Zubaidah Zakaria (2012). Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report. Journal of Medical Case Report 6(1): 30

19 Aliza MY, Nor Asiah M, Manaf AA, Chin YM, Normi M, Zubaidah Z and George E (2012). Prevalence and disease burden of common alpha thalassemia deletions in Malaysian blood donors: a multi ethnic population. International Journal of Scientific and Research Publications 2(4): 89-93

20 Wan Nazaimoon Wan Mohamud, Amir Sharifudin Mohd Khir, Aziz Al Safi Ismail, Ikram Shah Ismail,Kamarul Imran Musa, Khalid Abd Kadir, Nor Azmi Kamaruddin, Nor Azwany Yacob, Norlaila Mustafa, Osman Ali, Siti Harnida Mohd Isa, Wan Mohamad Wan Bebakar (2012). Prevalence of metabolic syndrome and its risk factors in adult Malaysians: Result of a nationwide survey. Diabetes Research and Clinical Practice 91: 239-245

21 Mansor Fazliana, Harvest F Gu, Claes-Goran Ostenson, Mashitah Mohd Yusoff and WM Wan Nazaimoon (2012). Labisia pumila extract down- regulates hydroxysteroid (11-beta) dehydrogenase 1 expression and corticosterone levels in ovariectomized rats. Journal of Natural Medicines 66(2): 257-264

22 Jeffery J, Sulaiman S, Oothuman P, Vellayan S, Zainol-Ariffin P, Paramaswaran S, Razak A, Muslimin M, Kamil-Ali OB, Rohela M and Abdul Aziz NM (2012). Domiciliary cockroaches found in restaurants in five zones of Kuala Lumpur Federal Territory, Peninsular Malaysia. Tropical Biomedicine 29(1): 180-186

23 Lim Kuang Hock, Sumarni Mohd Ghazali, Kee Chee Cheong, Hejar Abdul Rahman and Amal Nasir Mustafa (2012). Perceived norms and smoking status amobg secondary school students in Kota Tinggi, Johor, Malaysia. International Journal of Public Health Research 2(1): 85-92

24 Rahizan Issa, Athirah Abdul Sani, Nurul Akma Mohd Hassan and Norazah Ahmad (2012). Development of the quantitative Real-Time PCR assay for detection of Mycobacterium xenopi. Journal of Medical and Biological Sciences 5(1): 1-9

25 Mohd A Mohd Ridzuan, Robert W Moon, Ellen Knuepfer, Sally Black, Anthony A Holder and Judith L Green (2012). Subcellular location, phosphorylation and assembly into the motor complex of GAP45 during Plasmodium falciparum schizont development. PLosOne 7(3): e33845

44 26 Saraswathy TS, AS Santhana Raj, O Khairul Azuan, R Aida Suhana, A Izan Shahrina and S Zainah (2012). Electron microscopy studies of human adenovirus isolated from an acute respiratory disease outbreak in a Police Training Centre in Kuala Lumpur, Malaysia. Annals of Microscopy 12: 20-26

27 AWA Tan, SR Loke, S Benjamin, HL Lee, KH Chooi and M Sofian-Azirun (2012). Spray application of Bacillus thuringiensis israelensis (Bti strain AM65-52) against Aedes aegypti (L.) and Ae. Albopictus Skuse populations and impact on dengue transmission in a dengue endemic residential site in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 43(2): 296-310

28 Adlin Afzan, Noor Rain Abdullah, Siti Zaleha Halim, Badrul Amini Rashid, Raja Hazlini Raja Semail, Noordini Abdullah, Ibrahim Jantan, Hussin Muhammad and Zakiah Ismail (2012). Repeated dose 28-days oral toxicity study of Carica papaya L. leaf extract in sprague dawley rats. Molecules 17: 4326-4342

29 Mohd Apandi Yusof, Tengku Rogayah Tengku Abdul Rashid, Ravindran Thayan, Khairul Azuan Othman, Norhasnida Abu Hasan, Norfaezah Adnan and Zainah Saat (2012). Human A adenovirus type 7 outbreak in Police Training Center, Malaysia 2011. Emerging Infectious Diseases 18(5): 852-854

30 Lim KH, Idzwan MF, Sumarni MG, Kee C, Amal N, Lim K and Gurpeet K (2012). Heaviness of smoking index, number of cigarettes smoked and the fagerstrom test for nicotine dependence among adult male Malaysians. Asian Pacific Journal of Cancer Prevention 13(1): 343-346

31 Suzana S, Kee CC, Jamaluddin AR, Noor Safiza MN, Khor GL, Jamaiyah H, Geeta A, Ahmad Ali Z, Rahmah R, Ruzita AT and Ahmad Fauzi Y (2010). The Third National Health and Morbidity Survey: prevalence of obesity and abdominal obesity among the Malaysian elderly population. Asia Pacific Journal of Public Health 24(2): 318-329

32 Chun Lai Too, Abqariyah Yahya, Shahnaz Murad, Jasbir S Dhaliwal, Nor A Muhamad, Nor A Abdullah, Amal N Mustafa, Lars Klareskog, Lars Alfredson, Leonid Padyukov and Camilla Bengtsson (2012). Smoking interacts with HLA-DRB1 shared epitope in the development of ACPA- positive rheumatoid arthritis: results from the Malaysian Epidemiological Investigation of Rheumatoid Arthritis (MyEiRA). Arthritis Research and Theraphy 14(2):R89

45 33 Shanti Balasubramaniam, Lim Kia Suan, Fadhilah Mohd Jamil, Noor Kamila Abdullah and Norsiah Mohd Desa (2012). Isolated sulfite oxidase deficiency, a rare neurodegenerative disorders which hypoxic-ischemic encephalopathy. Journal of Pediatric Neurology 10(1)

34 Mohammed Morish, Yasmin Ayob, Noris Naim, Huda Salman, Nor Asiah Muhamad and Narazah Mohd Yusoff (2012). Quality indicator for discarding blood in the National Blood Center, Kuala Lumpur. Asian Journal of Transfusion Medicine 6(1): 19-23

35 AE Sima ,I Patimah, AR Sabariah, M Saidi, A Mohd. Yusoff (2012).The association of DNA damage level with early age at the occupational exposure in the mechanical workshops workers. Asian Journal of Biotechnology 4: 83-91

36 LS Chow, A Mohd Yusoff, AR Anita, SH Syed Tajuddin, I Kamal (2012). Prevalence of neck pain and associated factors with personal characteristics, physical workloads and psychosocial among male rubber workers in FELDA Settlement Malaysia. Global Journal of Health Science 4(1): 94-104

37 Yin-Hui Leong, Ahmad Rosma, Aishah A. Latif and A Nurul Izzah (2012). Association of serum aflatoxin B1-lysine adduct level with socio- demographic factors and dietary intake of aflatoxin in Malaysia. International Journal of Hygiene and Environmental Health 215(3): 368- 372

38 Yin-Hui Leong, Aishah A Latiff, Nurul Izzah Ahmad and Ahmad Rosma (2012). Exposure measurement of aflatoxins and aflatoxin metabolites in human body fluids. A short review. Mycotoxin Research 28(2): 79-87

39 SA Eshkoor, SJ Marashi, P Ismail, SA Rahman, M Mirinargesi, MY Adon and RV Devan (2012). Association of GSTM1 and GSTT1 with ageing in auto repair shop workers. Genetics and Molecular Research 11(2): 1486- 1496

40 Sujatha Ramasamy, Norhanom Abdul Wahab, Nurhayati Zainal Abidin, Sugumaran Manickam and Zubaidah Zakaria (2012). Growth inhibition of human gynecologic and colon cancer cells by Phyllanthus watsoniii through apoptosis induction. Plos One 7(4): e34793

41 Noraziah Nordin, Juriyati Jalil, Ibrahim Jantan and Shahnaz Murad (2012). Platelet-activating factor (PAF) receptor binding activity of the roots of Enicosanthellum pulchrum. Pharmaceutical Biology 50(3): 284- 290

46 42 Siti Roszilawati Ramli, Hui-min Neoh, Muhammad Nazri Aziz and Salasawati Hussin (2012). Screening and detection of heterogenous vancomycin intermediate Staphylococcus aureus in Hospital Kuala Lumpur, using the glycopeptide resistance detection Etest and population analysis profiling. Infectious Disease Report 4(1):e20 71-72

43 Stephen N Ponnampalam, Wilkinson YJ Tan, Nayyer Naveed Wazir and John George (2012). Unusual cause of neuropathy:extensive dural spread of primary cervical osteosarcoma. Acta Radiologica Short Reports 1(1): 6

47