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Dissertao De Mestrado
DISSERTAÇÃO DE MESTRADO Clonagem e expressão do cDNA codificante para a toxina do veneno de Lasiodora sp, LTx2, em vetor de expressão pET11a. Alexandre A. de Assis Dutra Ouro Preto, Julho de 2006 Universidade Federal de Ouro Preto Núcleo de Pesquisa em Ciências Biológicas Programa de Pós-graduação em Ciências Biológicas Universidade Federal de Ouro Preto Núcleo de Pesquisa em Ciências Biológicas Programa de Pós-graduação em Ciências Biológicas Clonagem e expressão do cDNA codificante para a toxina do veneno de Lasiodora sp, LTx2, em vetor de expressão pET11a. Alexandre A. de Assis Dutra ORIENTADOR: PROF. DR. IESO DE MIRANDA CASTRO Dissertação apresentada ao programa de pós-graduação do Núcleo de Pesquisa em Ciências Biológicas da Universidade Federal de Ouro Preto, como parte integrante dos requisitos para a obtenção do Título de Mestre em Ciências Biológicas na área de concentração Biologia Molecular. Ouro Preto, julho de 2006 D978c Dutra, Alexandre A. Assis. Clonagem e expressão do DNA codificante para a toxina do veneno de Lasiodora sp, LTx2, em vetor de expressão pET11a: [manuscrito]. / Alexandre A. Assis Dutra. - 2006. xi, 87f.: il., color; graf.; tabs. Orientador: Prof. Dr. Ieso de Miranda Castro. Área de concentração: Biologia molecular. Dissertação (Mestrado) - Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. 1. Clonagem - Teses. 2. Biologia molecular -Teses. 3. Toxinas - Teses. 4. Aranha - Veneno - Teses. I.Universidade Federal de Ouro Preto. Instituto -
Dna Sequence Data Indicates the Polyphyl Y of the Family Ctenidae (Araneae )
1993. The Journal of Arachnology 21 :194–201 DNA SEQUENCE DATA INDICATES THE POLYPHYL Y OF THE FAMILY CTENIDAE (ARANEAE ) Kathrin C . Huber', Thomas S . Haider2, Manfred W . Miiller2, Bernhard A . Huber' , Rudolf J. Schweyen2, and Friedrich G . Barth' : 'Institut fair Zoologie, Althanstr . 14; 1090 Wien; and 2lnstitut fur Mikrobiologie and Genetik; Dr. Bohrgasse 9 ; 1030 Wien (Vienna), Austria . ABSTRACT. Mitochondrial DNA fragments comprising more than 400 bases of the 16S rDNA from nine spider species have been sequenced: Cupiennius salei, C. getazi, C. coccineus and Phoneutria boliviensis (Ctenidae), Pisaura mirabilis, Dolomedes fimbriatus (Pisauridae), Pardosa agrestis (Lycosidae), Clubiona pallidula (Clubi- onidae) and Ryuthela nishihirai (syn. Heptathela nishihirai; Heptathelidae: Mesothelae). Sequence divergence ranges from 3–4% among Cupiennius species and up to 36% in pairwise comparisons of the more distantly related spider DNAs. Maximally parsimonious gene trees based on these sequences indicate that Phoneutri a and Cupiennius are the most distantly related species of the examined Lycosoidea . The monophyly of the family Ctenidae is therefore doubted ; and a revision of the family, which should include DNA-data, is needed . Cupiennius salei (Ctenidae) is one of the most get a high copy number of the DNA segment of extensively studied species of spiders (see Lach - interest. The PCR depends on the availability of muth et al. 1985). The phylogeny of the Ctenidae , oligonucleotides that specifically bind to the a mainly South and Central American family, i s flanking sequences of this DNA segment. These poorly understood ; and systematists propose oligonucleotides serve as primers for a polymer- highly contradicting views on its classification ization reaction that copies the segment in vitro. -
Redescription and Notes on the Natur Iption and Notes on the Natur Iption
Redescription and notes on the natural history of the arboreal tarantula Iridopelma seladonium (Araneae: Theraphosidae: Aviculariinae) Lina M. Almeida-Silva 1; Agustín Camacho 2; Antonio D. Brescovit 1; Sylvia M. Lucas 1 & Tania K. Brazil 3 1 Laboratório de Artrópodes, Instituto Butantan, Butantan. Avenida Vital Brazil, 1500, 05503-900 São Paulo, São Paulo, Brasil. E-mail: [email protected]; [email protected]; [email protected] 2 Laboratório de Herpetologia, Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo. Caixa Postal 11461, 05422-970 São Paulo, São Paulo, Brasil. E-mail: [email protected] 3 Laboratório de Animais Peçonhentos, Universidade Federal da Bahia. Avenida Ademar de Barros, Ondina, 40170-115 Salvador, Bahia, Brasil. E-mail: [email protected] ABSTRACT. Iridopelma seladonium (C.L. Koch, 1841) (Theraphosidae, Aviculariinae) is a very rare, colorful and small tarantula found in the Atlantic Rainforest in Bahia and Sergipe. The original description of this species was based on a single female and no illustrations of reproductive organs were presented. After we collected several specimens identified as I. seladonium, we decided to redescribe this species and discovered that the male, previously described as belonging to this species, was misidentified by MELLO LEITÃO (1923). Thus, in this paper the male of I. seladonium is newly described and the reproductive organs of male and female are described and illustrated in detail. Notes on the behavior and natural history of I. seladonium are presented based on the observation of live specimens in the field and in captivity. An immature of I. seladonium was observed constructing a hinged retreat with silk and little pieces of bark, a behavior not previously seen for other Aviculariinae. -
Psalmopoeus Cambridgei (Trinidad Chevron Tarantula)
UWI The Online Guide to the Animals of Trinidad and Tobago Ecology Psalmopoeus cambridgei (Trinidad Chevron Tarantula) Order: Araneae (Spiders) Class: Arachnida (Spiders and Scorpions) Phylum: Arthropoda (Arthropods) Fig. 1. Trinidad chevron tarantula, Psalmopoeus cambridgei. [http://www.exoreptiles.com/my/index.php?main_page=product_info&products_id=1127, downloaded 30 April 2015] TRAITS. A large spider, maximum size 11-14cm across the legs, with chevrons (V-shaped marks) on the abdomen (Fig. 1). Males are either grey or brown in colour, and females vary from green to brown with red or orange markings on the legs (Wikipedia, 2013). The Trinidad chevron tarantula is hairy in appearance, has eight legs, and its body is divided into two parts, the cephalothorax and the abdomen which are connected by a pedicel that looks like a narrow stalk (Fig. 2). The cephalothorax has eight legs plus a pair of smaller leg-like appendages (pedipalps) used to catch prey; in males these have palpal bulbs attached to the ends for holding sperm (Fig. 3). The mouth has chelicerae with fangs at the ends and swollen bases that house the venom glands, and there are eight small eyes (Foelix, 2010). However, even with eight eyes the Trinidad chevron tarantula can hardly see and so depends mostly on touch, smell, and taste to find its way. There are organs on their feet to detect changes in the environment and special type of hair on their legs and pedipalps for taste. The second part, the abdomen attached to a narrow waist, can UWI The Online Guide to the Animals of Trinidad and Tobago Ecology expand and contract to accommodate food and eggs; two pairs of spinnerets are located at the end of the abdomen (Fig. -
Potent Neuroprotection After Stroke Afforded by a Double-Knot Spider-Venom Peptide That Inhibits Acid-Sensing Ion Channel 1A
Potent neuroprotection after stroke afforded by a double-knot spider-venom peptide that inhibits acid-sensing ion channel 1a Irène R. Chassagnona, Claudia A. McCarthyb,c, Yanni K.-Y. China, Sandy S. Pinedaa, Angelo Keramidasd, Mehdi Moblie, Vi Phamb,c, T. Michael De Silvab,c, Joseph W. Lynchd, Robert E. Widdopb,c, Lachlan D. Rasha,f,1, and Glenn F. Kinga,1 aInstitute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia; bBiomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia; cDepartment of Pharmacology, Monash University, Clayton, VIC 3800, Australia; dQueensland Brain Institute, The University of Queensland, St. Lucia, QLD 4072, Australia; eCentre for Advanced Imaging, The University of Queensland, St. Lucia, QLD 4072, Australia; and fSchool of Biomedical Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia Edited by Solomon H. Snyder, Johns Hopkins University School of Medicine, Baltimore, MD, and approved February 6, 2017 (received for review September 1, 2016) Stroke is the second-leading cause of death worldwide, yet there are extracellular pH that occurs during cerebral ischemia. ASIC1a is the no drugs available to protect the brain from stroke-induced neuronal primary acid sensor in mammalian brain (9, 10) and a key mediator of injury. Acid-sensing ion channel 1a (ASIC1a) is the primary acid sensor stroke-induced neuronal damage. Genetic ablation of ASIC1a reduces in mammalian brain and a key mediator of acidosis-induced neuronal infarct size by ∼60% after transient middle cerebral artery occlusion damage following cerebral ischemia. Genetic ablation and selective (MCAO) in mice (7), whereas pharmacologic blockade with modestly pharmacologic inhibition of ASIC1a reduces neuronal death follow- potent ASIC1a inhibitors, such as amiloride (7) and nonsteroidal anti- ing ischemic stroke in rodents. -
Pet Health and Happiness Is Our Primary Concern
Pet Health and Happiness Is Our Primary Concern CONCISE CARE SHEETS PINK-TOES find more caresheets at nwzoo.com/care AND TREE SPIDERS INTRO QUICK TIPS PSALMOPOEUS The tropics of the New World or Americas are home to many popular The Trinidad Chevron (Psalmopoeus tree-dwelling tarantulas. These include a wide variety of “Pink-toes” of the » 76-82°F with a drop in tem- cambridgei] and Venezuelan Suntiger (P. genus Avicularia, the closely related Antilles “pink-toe” or tree tarantula perature at night (72-76ºF) irminia) are the best known members of (Caribena versicolor), the lightning-fast and agile Tapinauchenius and » Requires 70-80% humidity, a genus popular with tarantula keepers. a handful of species of Psalmopoeus like the Venezuelan Suntiger and but also good ventilation. Both are fairly large (P. cambridgei can Trinidad Chevron. » Most species will eat a reach a legspan of seven inches) and variety of arthropods and make exceptional display subjects for These tarantulas are found in a variety of subtropical and tropical habitats, small vertebrates yet thrive the spacious vertically-oriented forest but their general care is similar enough to cover them all in a single care on roaches or crickets in terrarium. Psalmopoeus tarantulas are sheet. Optimal captive husbandry is focused on providing warm, humid captivity. very hardy and often more forgiving of air in an enclosure that allows for sufficient ventilation. It is a balancing drier conditions than other New World act of sorts, but the conscientious and cautious keeper soon learns to err arboreal tarantulas. Both the Chevron on the side of good airflow as it is easier to add moisture than to correct and Suntiger appreciate a couple of layers of vertical cork bark slabs to overly damp or stagnant conditions. -
Vol. 9(1), 2016 ISSN 1675 – 5650
Vol. 9(1), 2016 ISSN 1675 – 5650 MALAYSIAN COCOA JOURNAL ------------------------------------------------------------ Advisor Datin Norhaini Udin Vice Advisor Dr. Ramle Kasin Editor Dr. Rosmin Kasran Editorial Committee Dr. Alias Awang Dr. Ahmad Kamil Mohd Jaaffar Dr. Douglas Furtek Dr. Fisal Ahmad Dr. Hii Ching Lik Dr. Rozita Osman Dr. Samuel Yap Kian Chee Dr. Tan Chia Lock Dr. Zainal Baharum En. Mohamed Yusof Ishak Hjh. Winoryantie Sulaiman Pn. Harnie Harun Pn. Suzannah Sharif Published by MALAYSIAN COCOA BOARD 5-7th Floor, Wisma SEDCO Lorong Plaza Wawasan, Off Coastal Highway 88999 Kota Kinabalu, Sabah, Malaysia ©All rights reserved. No part of this publication may be reproduced in any form or by any means without permission in writing from Malaysian Cocoa Board CONTENTS ASSESSMENT OF SOMATIC EMBRYOGENESIS POTENTIAL OF ELITE MALAYSIAN Theobroma cacao CLONES 1 Siti Norhana, M. A., Haikal Eman, A., Ahmad Kamil, M. J. and Kasran, R. THE EFFECT OF ASCORBIC ACID IN THE MATURATION OF COCOA SOMATIC EMBRYOS 5 Norasekin, T. and Ducos, J.P. IMPROVEMENTS OF Theobroma cacao ACCLIMATIZATION TO EX VITRO ENVIRONMENT 11 Nik Aziz, N. I., Jasli, M., Misak, E., Abdul Asui, N. and Kasran, R. DEVELOPMENT OF SNP GENOTYPING ASSAYS IN COCOA POD BORER USING REAL-TIME PCR Kasran, R., Roslina, M.S., David, A., Nuraziawati, M.Y., Navies, M.., Mellisa, G., Fahmi, W. and 19 Larry, C. IDENTIFICATION OF THE POTENTIAL RESISTANCE GENES IN Theobroma cacao TO COCOA POD BORER INSECT (Conopomorpha cramerella) IN RELATION TO HOST- PATHOGEN INTERACTION USING RNA-SEQ TECHNOLOGY 40 Roslina, M.S., David, A., Anisah, S., Navies, M., Kasran, R., K., Mellisa, G.,Fahmi, W. -
Taxonomical Revision & Cladistic Analysis of Avicularia
Caroline Sayuri Fukushima Taxonomical revision & cladistic analysis of Avicularia Lamarck 1818 (Araneae, Theraphosidae, Aviculariinae). Thesis presented at the Institute of Biosciences of the University of Sao Paulo, to obtain the title of Doctor of Science in the field of Zoology. Adviser (a): Paulo Nogueira-Neto Corrected version Sao Paulo 2011 (the original version is available at the Biosciences Institute at USP) Fukushima, Caroline Sayuri Taxonomical revision & cladistic analysis of Avicularia Lamarck 1818 (Araneae, Theraphosidae, Aviculariinae). 230 Pages Thesis (Ph.D.) - Institute of Biosciences, University of Sao Paulo. Department of Zoology. 1. Avicularia 2. Theraphosidae 3. Araneae I. University of Sao Paulo. Institute of Biosciences. Department of Zoology. Abstract The genus Avicularia Lamarck 1818 contains the oldest mygalomorph species described. It’s taxonomical history is very complex and for the first time it has been revised. A cladistic analysis with 70 characters and 43 taxa were done. The preferred cladogram was obtained using the computer program Pee Wee and concavity 6. The subfamily Aviculariinae contains the genera Stromatopelma, Heteroscodra, Psalmopoeus, Tapinauchenius, Ephebopus, Pachistopelma, Iridopelma, Avicularia, Genus 1 and Gen. nov. 1, Gen. nov. 2, Gen. nov. 3 and Gen. nov.4. Aviculariinae is monophyletic, sharing the presence of spatulated scopulae on tarsi and metatarsi, juveniles with a central longitudinal stripe connected with lateral stripes on dorsal abdomen and arboreal habit. The synapomorphy of Avicularia is the presence of a moderately developed protuberance on tegulum. The genus is constituted by 14 species: A. avicularia (type species), A. juruensis, A. purpurea, A. taunayi, A. variegata status nov., A. velutina, A. rufa, A. aymara, Avicularia sp. -
Tarantulas and Social Spiders
Tarantulas and Social Spiders: A Tale of Sex and Silk by Jonathan Bull BSc (Hons) MSc ICL Thesis Presented to the Institute of Biology of The University of Nottingham in Partial Fulfilment of the Requirements for the Degree of Doctor of Philosophy The University of Nottingham May 2012 DEDICATION To my parents… …because they both said to dedicate it to the other… I dedicate it to both ii ACKNOWLEDGEMENTS First and foremost I would like to thank my supervisor Dr Sara Goodacre for her guidance and support. I am also hugely endebted to Dr Keith Spriggs who became my mentor in the field of RNA and without whom my understanding of the field would have been but a fraction of what it is now. Particular thanks go to Professor John Brookfield, an expert in the field of biological statistics and data retrieval. Likewise with Dr Susan Liddell for her proteomics assistance, a truly remarkable individual on par with Professor Brookfield in being able to simplify even the most complex techniques and analyses. Finally, I would really like to thank Janet Beccaloni for her time and resources at the Natural History Museum, London, permitting me access to the collections therein; ten years on and still a delight. Finally, amongst the greats, Alexander ‘Sasha’ Kondrashov… a true inspiration. I would also like to express my gratitude to those who, although may not have directly contributed, should not be forgotten due to their continued assistance and considerate nature: Dr Chris Wade (five straight hours of help was not uncommon!), Sue Buxton (direct to my bench creepy crawlies), Sheila Keeble (ventures and cleans where others dare not), Alice Young (read/checked my thesis and overcame her arachnophobia!) and all those in the Centre for Biomolecular Sciences. -
Despre Tarantule – Posibile Utilizări În Medicină About Tarantula - Possible Uses in Medicine
Cîmpian și Cristina. Medicamentul Veterinar / Veterinary Drug Vol. 12(2) Decembrie 2018 Despre tarantule – posibile utilizări în medicină About tarantula - possible uses in medicine Diana Cîmpian, Romeo Teodor Cristina Facultatea de Medicină Veterinară Timișoara Cuvinte cheie: biologie, tarantule, venin, structură, utilizări Key words: biology, tarantulas, venom, structure, uses Rezumat În ultimii ani tarantulele au devenit tot mai populare în teraristică datorită faptului că sunt ușor de întreținut, nu necesită mult spațiu și datorită frumuseții lor. Veninul de la păianjeni, șerpi, pești, melci și scorpioni conțin o farmacopee evoluată a toxinelor naturale care vizează receptorii membranari și canalele de ioni pentru a produce șoc, paralizie, durere sau deces. Veninurile de tarantulă reprezintă una dintre cele mai mari colecții de combinații de compuși chimici din lume. Acestea au fost dezvoltate în mod selectiv pentru a genera în final niște structuri bioactive extrem de puternice, selective, care au fost supuse unui proces de optimizare naturală prin milioanele de ani de selecție naturală. Studiul diverselor tipuri de venin a devenit, în ultimii ani, o prioritate pentru științele medicale și biologie. În acest sens, medicii veterinari sunt chemați să cunoască tarantulele ca ființe, modul lor de viață, de hrănire, de înmulțire precum și modul de obținere, conservare, analiză și utilizare a veninurilor. Lucrarea aduce informații despre viața tarantulelor, modul de contenție și mai ales despre cum se recoltează și se stochează veninul de la această specie. Abstract In recent years, tarantulas have become increasingly popular in terrariums because they are easy to maintain, do not require much space and because of their beauty. Venom from spiders, snakes, fish, snails and scorpions contain an advanced pharmacopoeia of natural toxins that target membrane receptors and ion channels to produce shock, paralysis, pain or death. -
WO 2017/035099 Al 2 March 2017 (02.03.2017) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/035099 Al 2 March 2017 (02.03.2017) P O P C T (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, C07C 39/00 (2006.01) C07D 303/32 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, C07C 49/242 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (21) International Application Number: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PCT/US20 16/048092 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (22) International Filing Date: SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 22 August 2016 (22.08.2016) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (30) Priority Data: TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 62/208,662 22 August 2015 (22.08.2015) US TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (71) Applicant: NEOZYME INTERNATIONAL, INC. -
Hábito Bromelícola De Corinna Sp
UNIVERSIDADE ESTADUAL PAULISTA INSTITUTO DE BIOCIÊNCIAS LETRAS E CIÊNCIAS EXATAS SÃO JOSÉ DO RIO PRETO – SP PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA ANIMAL GUSTAVO CAUÊ DE OLIVEIRA PICCOLI HISTÓRIA NATURAL DA ARANHA CORINNA SP. NOV. (CORINNIDAE): INTERAÇÕES COM BROMÉLIAS E COMPORTAMENTO DE SUBMERSÃO EM FITOTELMATA DISSERTAÇÃO APRESENTADA PARA OBTENÇÃO DO TÍTULO DE MESTRE EM BIOLOGIA ANIMAL, ÁREA DE ECOLOGIA E COMPORTAMENTO DO INSTITUTO DE BIOCIÊNCIAS, LETRAS E CIÊNCIAS EXATAS DA UNIVERSIDADE ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO”, CAMPUS DE SÃO JOSÉ DO RIO PRETO ORIENTADOR: Dr. GUSTAVO QUEVEDO ROMERO - 2011 - Piccoli, Gustavo Cauê de Oliveira. História natural da aranha Corinna sp. nov. (Corinnidae) : interações com bromélias e comportamento de submersão de fitotelmatas / Gustavo Cauê de Oliveira Piccoli. - São José do Rio Preto : [s.n.], 2011. 75 f. : il. ; 30 cm. Orientador: Gustavo Quevedo Romero Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas 1. Ecologia animal. 2. Aracnídeo – História natural. 3. Aranha – Ecologia. 4. Bromeliacea - Comportamento. I. Romero, Gustavo Quevedo. II. Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas. III. Título. CDU – 595.4 Ficha catalográfica elaborada pela Biblioteca do IBILCE Campus de São José do Rio Preto - UNESP Data da defesa: 18 de fevereiro de 2011 BANCA EXAMINADORA TITULARES: Dr. Gustavo Quevedo Romero (Orientador) Universidade Estadual de Campinas - SP Prof. Dr.. João Vasconcellos-Neto Universidade