Poster Abstracts
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19th April 8th - 11th 2019 at the Oxford Conference Center | 102 Ed Perry Blvd, Oxford, Mississippi The Oxford International Conference on the Science of Botanicals is an annual meeting to discuss approaches for post market surveillance, risk and safety assessment, quality control and adverse event reporting (AER) for botanical dietary supplements (BDS) and natural products as well as regulatory aspects with perspectives from government, manufacturers and trade associations Celebrating 25 Years of DSHEA SUPPLE RY M e upcoming year’s meeting will celebrate the 25th Anniversary A E T N E T I of DSHEA (e Dietary Supplement Health and Education D H E A T L Act of 1994) by reviewing the history, confronting the C T A H N A O issues, and discussing future aspects. I N T D A E C D 25U Poster Abstracts TITLE SPONSORSHIP PROVIDED BY: http://oxfordicsb.org | https://facebook.com/OxfordICSB | [email protected] | Phone: (662) 915-7821 | Fax: (662) 915-7989 PA1 Determining Endogenous Thresholds for Athletically Banned Substances Rebecca E. Adams1 and John C. Travis1 1NSF International, Ann Arbor, MI, USA Dietary supplements are a multibillion dollar industry. Key stakeholders in this industry include companies that produce sports supplements formulated for athletes. Certain substances are prohibited or banned from sport by organizations including the World Anti-Doping Agency (WADA), National Football League (NFL) and Major League Baseball (MLB). Chemicals/substances are placed on the WADA list when they meet at least two of the following criteria: 1) the substance has the potential to enhance performance, 2) the substance has an adverse effect risk, and 3) the substance violates the spirit of sport. By prohibiting these chemicals, their acceptability criterion in a finished product dietary supplement is represented by the absence of the substance at the analytically feasible detection threshold. Importantly, there are instances where prohibited substances are endogenous in plants or animals, and the endogenous nature of these substances should be accounted for when setting thresholds. Recently, an athletically banned substance, Boldione, was reviewed for its endogenous potential. Boldione is currently listed by WADA as an endogenous anabolic androgenic steroid (AAS) when administered exogenously. Although Boldione is not a phytochemical constituent of botanicals, literature is available describing biotransformation of the phytosterol, β-sitosterol, to Boldione in the presence of mycobacterium. Given that Boldione can be produced endogenously in plants, an endogenous threshold of 0.02 µg per serving was established using endogenous Boldione levels in human urine (1.33 ng/ml x 50 ml urine) multiplied by the maximum excretion rate of Boldione in urine (34.45%). Typical performance enhancing use levels of AAS are roughly 25 mg per day with dosage levels as low as 1.14 mg per day. Additionally, a maximum allowable level of 0.3 mg per day was derived from a subchronic oral study wherein rats were administered the AAS androstenedione. These values are orders of magnitude higher than the Boldione per serving endogenous threshold. Thus, derivation of a risk-based acceptability criteria threshold for Boldione does not violate anti-doping requirements and the endogenous nature of the athletically banned substance, Boldione, should be accounted for when setting an acceptability criteria threshold. PA2 Isolation of three new flavones from Callistemon lanceolatus and assessment of their biological activity: In vivo hypoglycemic effect and in vitro PPAR- γ transactivation activity. Syed Nazreen1,5, Mohammad Sarwar Alam1*, Hinna Hamid1, Abhijeet Dhulap4, Mohammad Mahboob Alam1,5, Sameena Bano1, Saqlain Haider1,6, Mohammad Ali2 and K K Pillai3 1Department of Chemistry, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi-110062, India; 2Department of Pharmacognosy and Phytochemistry, School oh Pharmaceutical Education and Research, Jamia Hamdard, New Delhi-110062, India; 3Department of Pharmacology, School oh Pharmaceutical Education and Research, Jamia Hamdard, New Delhi-110062, India; 4CSIR unit for Research and Development of Information Products, Pune 411038, India; 5Department of Chemistry, Faculty of Science, University of Al baha, Kingdom of Saudi Arabia; 6National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, USA. *Corresponding and presenting author (Email ID- [email protected]. Cell: +91 9717927759 Callistemon lanceolatus DC (Myrtaceae) is commonly known as ‘red bottle brush’ plant. The hypoglycaemic activity of the ethanolic extractof the leaves of Callistemon lanceolatus as well as its petroleum ether and chloroform fractions were studied in streptozotocin induced diabetic rats for 30 days. The effect on biochemical parameters viz lipid peroxidation, reduced glutathione levels, activity of antioxidant enzymes etc were also assessed to evaluate their activity in controlling diabetes related metabolic conditions. The results indicate that the chloroform fraction at 150 mg/kg b.w. significantly lowers blood glucose level with insignificant ulceration compared to the standard drug glibenclamide (3 mg/kg b.w.). Also, there was reduction in lipid peroxidation level and reduced glutathione levels and elevation in the activity of antioxidant enzymes. The phytochemical investigation of the active chloroform fraction led to the isolation of three new flavones with significant hypoglycemic effect in streptozotocin induced diabetic rats in addition to two phenolic esters which have been www.oxfordicsb.org | facebook.com/OxfordICSB | [email protected] characterized using chemical and spectroscopic methods. One of the flavones besides exhibitingsignificant in vivo blood glucose lowering effect and without causing any toxic effect on the pancreas and liver also showed a higher glide score in comparison to the reference molecule (rosiglitazone) against PPAR-γ target in molecular docking studies. Moreover it also exhibited moderate in vitro PPAR- γ transactivation activity in comparison to the standard drugs, rosiglitazone and pioglitazone. The results of this study validates the traditional use of the leaves of Callistemon lanceolatus in the treatment of diabetes in folk medicine. The authors thank the Vice Chancellor Prof. (Dr) Seyed Ehtesham Hasnain and the former Vice Chancellor Dr G N Qazi for providing all necessary facilities to the Department of Chemistry. Syed Nazreen acknowledges University Grant Commission, Government of India for providing financial assistance. PA3 Rapid Screening and Quantitative Analysis of Amines/Alkaloids from Weight Loss and Pre-Workout Supplements using UHPLC-QToF 1 1 1 1 1 1, 2 Avula B , Bae J-Y , Chittiboyina AG , Wang Y-H , Wang M , Khan IA 1National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA. 2Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA. The use of supplements for weight loss and in sports as pre-workout products is wide spread. Many of these supplements were found to contain active components, which were not claimed on the labels. A validated ultra-high performance liquid chromatography high-resolution mass spectrometry quadrupole time-of-flight (UHPLC-QToF-MS) method was developed for the simultaneous analysis of 111 stimulants, anorectics and other active components including phenethylamines, amphetamines, sibutramine or yohimbine. This method involves the detection of [M+H]+ ions and the separation was achieved using a C18 column, water/acetonitrile gradient as the mobile phase. The method was validated for linearity, repeatability, accuracy, stability, system suitability, limits of quantification (LOQ) and limits of detection (LOD). The limits of detection were in the range from 0.001-0.5 µg/mL. The validated method was applied to the analysis of twenty-seven weight loss and pre-workout dietary supplements. Two-thirds of the supplements contained compounds that were not listed on the label. These include several phenethylamines (PEA) such as demelverine, hordenine, N, N-dimethyl-phenethylamine, synephrine, N-methyl-b-phenethylamine, and methylsynephrine. In addition, the PEA mimics such as dimethylamylamine, dimethylbutylamine other stimulants including fursultiamine, evodiamine, phenibut and theophylline were also observed. One or more of the ingredients listed on the labels were not detected in forty-four percent of the products analyzed. Positive identification was based on retention time, accurate mass and fragment ions in comparison with the respective reference standards. Development of such methods are anticipated to be of aid to regulatory agencies for the identification of xenobiotics commonly found in many dietary supplements. This research is supported in part by “Science Based Authentication of Dietary Supplements” funded by the Food and Drug Administration [grant number 5U01FD004246-07], and “Discovery and Development of Natural Products for Pharmaceutical and Agricultural Applications” funded by the United States Department of Agriculture [No. 58-6060-6- 015]. The authors would also like to thank Dr. Jon F. Parcher for his help in correcting grammar and support. www.oxfordicsb.org | facebook.com/OxfordICSB | [email protected] PA4 The Power of Hyphenated Chromatography - Time of Flight Mass Spectrometry for Unequivocal Identification of Spirostanes in Bodybuilding Dietary