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US Department of Commerce Noaa NATIONAL OCEANIC and ATMOSPHERIC ADMINISTRATION

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US Department of Commerce Noaa NATIONAL OCEANIC and ATMOSPHERIC ADMINISTRATION NOAA Technical Memorandum NOS ORCA 130 National Status and Trends Program for Marine Environmental Quality Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update Silver Spring, Maryland March 1998 US Department of Commerce noaa NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION Coastal Monitoring and Bioeffects Assessment Division Office of Ocean Resources Conservation and Assessment National Ocean Service Coastal Monitoring and Bioeffects Assessment Division Office of Ocean Resources Conservation and Assessment National Ocean Service National Oceanic and Atmospheric Administration U.S. Department of Commerce N/ORCA2, SSMC4 1305 East-West Highway Silver Spring, MD 20910 Notice This report has been reviewed by the National Ocean Service of the National Oceanic and Atmospheric Administration (NOAA) and approved for publication. Such approval does not signify that the contents of this report necessarily represent the official position of NOAA or of the Government of the United States, nor does mention of trade names or commercial products constitute endorsement or recommendation for their use. NOAA Technical Memorandum NOS ORCA 130 Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update G. G. Lauenstein and A. Y. Cantillo (Editors) Silver Spring, Maryland March 1998 United States National Oceanic and Department of Commerce Atmospheric Administration National Ocean Service William M. Daley D. James Baker Nancy Foster Secretary Under Secretary Assistant Administrator TABLE OF CONTENTS LIST OF TABLES..................................................................................................................i LIST OF FIGURES.................................................................................................................iii LIST OF ACRONYMS............................................................................................................iv Introduction G. G. Lauenstein and A. Y. Cantillo ABSTRACT.........................................................................................................................1 1. DISCUSSION..................................................................................................................1 2. BIBIOGRAPHY/REFERENCES...........................................................................................11 ANCILLARY METHODS Dry Weight Determination of Sediments S. T. Sweet and T. L. Wade ABSTRACT.........................................................................................................................14 1. INTRODUCTION..............................................................................................................14 2. APPARATUS AND MATERIALS.......................................................................................14 2.1. Equipment...................................................................................................14 2.2. Reagents....................................................................................................14 3. PROCEDURE..................................................................................................................14 4. CALCULATIONS.............................................................................................................15 4.1. Percent dry weight.....................................................................................15 4.2. Relative Percent Difference between duplicates...........................................15 5. QUALITY CONTROL........................................................................................................15 6. CONCLUSIONS...............................................................................................................15 Determination of Percent Dry Weight for Tissues Y. Qian and T. L. Wade ABSTRACT.........................................................................................................................16 1. INTRODUCTION..............................................................................................................16 2. APPARATUS AND MATERIALS.......................................................................................16 2.1. Apparatus..................................................................................................16 2.2. Labware.....................................................................................................16 2.3. Solvents and reagents.................................................................................17 3. PROCEDURE..................................................................................................................17 4. CALCULATIONS.............................................................................................................18 4.1. Percent dry weight.....................................................................................18 4.2. Relative percent difference between duplicates...........................................18 5. QUALITY CONTROL........................................................................................................18 6. CONCLUSIONS...............................................................................................................18 Sediment Grain Size Analysis - Gravel, Sand, Silt and Clay S. T. Sweet, S. Laswell, and T. L. Wade ABSTRACT.........................................................................................................................19 1. INTRODUCTION..............................................................................................................19 2. SAMPLE COLLECTION, PRESERVATION AND STORAGE.....................................................19 2.1. Sample collection........................................................................................19 2.2. Sample preservation and storage................................................................19 3. APPARATUS AND MATERIALS.......................................................................................19 3.1. Labware and apparatus...............................................................................19 3.2. Reagents....................................................................................................20 4. PROCEDURE..................................................................................................................20 4.1. Preparation of samples for dry-sieving and pipette analysis........................20 4.2. Size analysis of sand/gravel fraction by wet-sieving..................................20 4.3. Silt/clay sized material by settling.............................................................21 5. CALCULATIONS.............................................................................................................21 6. CONCLUSIONS...............................................................................................................21 7. REFERENCE...................................................................................................................22 Total Organic and Carbonate Carbon Content of Sediments S. T. Sweet and T. L. Wade ABSTRACT.........................................................................................................................23 1. INTRODUCTION..............................................................................................................23 2. APPARATUS AND MATERIALS.......................................................................................23 2.1 Equipment.........................................................................................................23 2.2 Reagents..........................................................................................................24 3. Procedure....................................................................................................................24 3.1. LECO system preparation............................................................................24 3.2. Total carbon determination.........................................................................24 3.2.1. Sample preparation.........................................................................24 3.2.2. Sample analyses.............................................................................24 3.2.3. Standard analyses..........................................................................25 3.3. Total Organic Carbon determination.............................................................25 3.3.1. Sample preparation.........................................................................25 3.3.2. Sample analyses.............................................................................25 3.4. Total carbonate carbon content...................................................................25 4. STANDARDIZATION AND CALCULATIONS........................................................................25 5. QUALITY CONTROL........................................................................................................26 6. REPORTING AND PERFORMANCE CRITERIA......................................................................26 7. CONCLUSIONS...............................................................................................................26 Determination of Percent Lipid in Tissue Y. Qian, J. L. Sericano, and T. L. Wade ABSTRACT.........................................................................................................................27
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