Protists and Fungi

Home , Asterophora parasitica, Microfungi

Protists and Fungi

Douglas Wilkin, Ph.D. Jean Brainard, Ph.D.

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Printed: September 26, 2014 www.ck12.org Chapter 1. Protists and Fungi

CHAPTER 1 Protists and Fungi

CHAPTER OUTLINE 1.1 Protist Kingdom 1.2 Protist Evolution 1.3 Protist Characteristics 1.4 Protozoa 1.5 Algae 1.6 Molds 1.7 Protists and Human Disease 1.8 Fungi 1.9 Fungi Structure 1.10 How Fungi Eat 1.11 Fungi Reproduction 1.12 Fungi Evolution 1.13 Fungi Classiﬁcation 1.14 Symbiotic Relationships of Fungi 1.15 Human Uses of Fungi 1.16 Fungi and Human Disease 1.17 References

Introduction

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Would you eat this mushroom? Now that’s an interesting mushroom! In fact, fungi can be some of the most colorful species. And protists can be some of the most interesting. These two kingdoms which have some of the most diverse members are the first two eukaryotic kingdoms we discuss in the Protists and Fungi chapter. This quintessential toadstool, the fly agaric or fly Amanita, is a poisonous and psychoactive fungus. It has a large white-gilled, white-spotted, usually deep red mushroom, and it is one of the most recognizable and widely encountered in popular culture. You should not eat it.

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1.1 Protist Kingdom

• Describe the protist kingdom.

Prokaryote or eukaryote? This organism consists of a single cell with several ﬂagella. Is it a prokaryote, such as a bacterium? Actually, it’s larger than a prokaryotic cell, and it also has a nucleus. Therefore, this organism belongs to the domain Eukarya, the domain that includes humans. This particular eukaryote is one of the smallest, simplest organisms in the domain, called a protist. It’s scientiﬁc name is Giardia lamblia. As a human parasite, it can make us sick.

Kingdom Protista

Protists are a group of all the eukaryotes that are not fungi, animals, or plants. As a result, it is a very diverse group of organisms. The eukaryotes that make up this kingdom, Kingdom Protista, do not have much in common besides a relatively simple organization. Protists can look very different from each other. Some are tiny and unicellular, like an amoeba, and some are large and multicellular, like seaweed. However, multicellular protists do not have highly specialized tissues or organs. This simple cellular-level organization distinguishes protists from other eukaryotes, such as fungi, animals, and plants. There are thought to be between 60,000 and 200,000 protist species, and many have yet to be identiﬁed. Protists live in almost any environment that contains liquid water. Many protists, such as the algae, are photosynthetic and are vital primary producers in ecosystems. Other protists are responsible for a range of serious human diseases, such as malaria and sleeping sickness. The term protista was ﬁrst used by Ernst Haeckel in 1866. Protists were traditionally placed into one of several groups based on similarities to a plant, animal, or fungus: the animal-like protozoa, the plant-like protophyta (mostly algae), and the fungus-like slime molds and water molds. These traditional subdivisions, which were

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largely based on non-scientiﬁc characteristics, have been replaced by classiﬁcations based on phylogenetics (evolutionary relatedness among organisms). However, the older terms are still used as informal names to describe the general characteristics of various protists.

FIGURE 1.1 Protists range from single-celled amoebas to multicellular seaweed. Protists may be similar to animals, plants, or fungi.

Summary

• Kingdom Protista includes all eukaryotes that are not animals, plants, or fungi. • Kingdom Protista is very diverse. It consists of both single-celled and multicellular organisms.

Explore More

Use this resource to answer the questions that follow.

• Kingdom Protista at http://www.biologycorner.com/lesson-plans/phyla/kingdom-protista/.

1. List three characteristics of protists. 2. How are protists classiﬁed? 3. What is another name for animal-like protists and plant-like protists? 4. Complete this statement: Any eukaryote that is not a plant, animal or fungus is a ______.

Explore More Answers

1. All protists are eukaryotic. Most are unicellular, but not all. Most live in water. 2. Protists are classiﬁed by how they obtain nutrition and how they move. 3. Animal-like Protists are also called protozoa. Plant-like Protists are also called alga. 4. Any eukaryote that is not a plant, animal or fungus is a protist.

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Review

1. What are protists? 2. How are unicellular protists and multicellular protists similar? 3. How are protists classiﬁed? What are the main categories of protists?

Review Answers

1. Protists are a group of all the eukaryotes that are not fungi, animals, or plants. 2. All protists, including multicellular protists, have a simple cellular-level organization. 3. Protists are classiﬁed by phylogenetics. The main categories of protists are the animal-like protozoa, the plant-like protophyta (mostly algae), and the fungus-like slime molds and water molds.

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1.2 Protist Evolution

• Outline the evolution of protists. • Explain the formation of the ﬁrst eukaryotic cells. • Site evidence for the endosymbiotic theory.

What’s the difference between a bacterium and a simple protist? Were simple protists the ﬁrst eukaryotic organisms to evolve? Probably. A protist is a eukaryote, so each cell has a nucleus. Otherwise, simple protists, like the Paramecium and amoeba, can be fairly similar to bacteria.

Evolution of Protists

Scientists think that protists are the oldest eukaryotes. If so, they must have evolved from prokaryotic cells. How did this happen? The endosymbiotic theory provides the most widely-accepted explanation. That’s because it is well supported by evidence.

The First Eukaryotic Cells

According to the endosymbiotic theory, the first eukaryotic cells evolved from a symbiotic relationship between two or more prokaryotic cells. Smaller prokaryotic cells were engulfed by (or invaded) larger prokaryotic cells. The small cells (now called endosymbionts) benefited from the relationship by getting a safe home and nutrients. The large cells (now called hosts) benefited by getting some of the organic molecules or energy released by the endosymbionts. Eventually, the endosymbionts evolved into organelles of the host cells. After that, neither could live without the other.

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As shown in Figure 1.2, some of the endosymbionts were aerobic bacteria. They were specialized to break down chemicals and release energy. They evolved into the mitochondria of eukaryotic cells. Some of the small cells were cyanobacteria. They were specialized for photosynthesis. They evolved into the chloroplasts of eukaryotic cells.

FIGURE 1.2 Endosymbiotic theory explains how eukaryotic cells arose.

Evidence for the Endosymbiotic Theory

Many pieces of evidence support the endosymbiotic theory. For example:

• Mitochondria and chloroplasts contain DNA that is different from the DNA found in the cell nucleus. Instead, it is similar to the circular DNA of bacteria. • Mitochondria and chloroplasts are surrounded by their own plasma membranes, which are similar to bacterial membranes. • New mitochondria and chloroplasts are produced through a process similar to binary ﬁssion. Bacteria also reproduce through binary ﬁssion. • The internal structure and biochemistry of chloroplasts is very similar to that of cyanobacteria.

Summary

• Scientists think that protists are the oldest eukaryotes. • Protists most likely evolved from prokaryotic cells, as explained by the endosymbiotic theory. This theory is well-supported by evidence.

Explore More

Use this resource to answer the questions that follow.

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• It Takes Teamwork: How Endosymbiosis Changed Life on Earth at http://evolution.berkeley.edu/evolib rary/article/endosymbiosis_01.

1. What were the predecessors of eukaryotic cells? 2. What is endosymbiosis? 3. What may have been the ﬁrst internal symbiotic relationship? Explain this relationship. 4. Why is DNA considered evidence for endosymbiosis?

Explore More Answers

1. Eukaryotic cells are actually the descendents of separate prokaryotic cells that joined together in a symbiotic union. 2. Endosymbiosis refers to an "internal" symbiosis in which one organism take up permanent residence inside another and eventually evolving into a single lineage. 3. The first internal symbiotic relationship was between two bacterium, the internal one which eventually became a mitochondria. In this relationship, a free-living bacterium was engulfed by another cell, perhaps as a meal, and ended up staying as a sort of permanent houseguest. The host cell profited from the chemical energy the mitochondrion produced, and the mitochondrion benefited from the protected, nutrient-rich environment surrounding it. 4. Each mitochondrion has its own circular DNA genome, like a bacteria’s genome, but much smaller. This DNA is passed from a mitochondrion to its offspring and is separate from the "host" cell’s genome in the nucleus.

Review

1. How did the ﬁrst eukaryotic cells evolve, according to endosymbiotic theory? 2. Identify two pieces of evidence for endosymbiotic theory. Explain how this evidence supports the theory. 3. How are additional mitochondria produced?

Review Answers

1. According to the endosymbiotic theory, the first eukaryotic cells evolved from a symbiotic relationship between two or more prokaryotic cells. Smaller prokaryotic cells were engulfed by (or invaded) larger prokaryotic cells. Eventually, the endosymbionts evolved into organelles of the host cells, creating, by definition, an eukaryotic cell. After that, neither could live without the other. 2. Two wo pieces of evidence for endosymbiotic theory - (1) Mitochondria and chloroplasts contain DNA that is different from the DNA found in the cell nucleus. Instead, it is similar to the circular DNA of bacteria. (2) Mitochondria and chloroplasts are surrounded by their own plasma membranes, which are similar to bacterial membranes. 3. Mitochondria (and chloroplasts) are produced through a process similar to binary fission.

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1.3 Protist Characteristics

• Identify protist characteristics. • List protist habitats. • Describe forms of protist motility. • Explain protist reproduction. • Summarize protist nutrition.

Sexual or asexual reproduction for protists? Notice how the Paramecium is dividing into two cells. This, obviously, is a form of asexual reproduction. But, remember that protists are an extremely diverse kingdom, and some protists can also reproduce sexually.

Characteristics of Protists

Like all other eukaryotes, protists have a nucleus containing their DNA. They also have other membrane-bound organelles, such as mitochondria and the endoplasmic reticulum. Most protists are single-celled. Some are multicellular. Because the protist kingdom is so diverse, their ways of getting food and reproducing vary widely.

Protist Habitats

Most protists are aquatic organisms. They need a moist environment to survive. They are found mainly in damp soil, marshes, puddles, lakes, and the ocean. Some protists are free-living organisms. Others are involved in symbiotic relationships. They live in or on other organisms, including humans.

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Motility of Protists

Most protists have motility. This is the ability to move. Protists have three types of appendages for movement. As shown in Figure 1.3, they may have flagella, cilia, or pseudopods (“false feet”). There may be one or more whip- like flagella. Cilia are similar to flagella, except they are shorter and there are more of them. They may completely cover the surface of the protist cell. Pseudopods are temporary, foot-like extensions of the cytoplasm.

FIGURE 1.3 Protists use cilia, pseudopods, or ﬂagella to move.

Protist Reproduction

Protists have complex life cycles. Many have both asexual and sexual reproduction. An example is a protist called Spirogyra, a type of algae, shown Figure 1.4. It usually exists as haploid cells that reproduce by binary ﬁssion. In a stressful environment, such as one that is very dry, Spirogyra may produce tough spores that can withstand harsh conditions. Spores are reproductive cells produced by protists and various other organisms. If two protist spores are close together, they can fuse to form a diploid zygote. This is a type of sexual reproduction. The zygote then undergoes meiosis, producing haploid cells that repeat the cycle.

FIGURE 1.4 Spirogyra is a genus of algae with a complex life cycle. Each organism consists of rectangular cells connected end-to-end in long ﬁlaments.

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Protist Nutrition

Protists get food in one of three ways. They may ingest, absorb, or make their own organic molecules.

• Ingestive protists ingest, or engulf, bacteria and other small particles. They extend their cell wall and cell membrane around the food item, forming a food vacuole. Then enzymes digest the food in the vacuole. • Absorptive protists absorb food molecules across their cell membranes. This occurs by diffusion. These protists are important decomposers. • Photosynthetic protists use light energy to make food. They are major producers in aquatic ecosystems.

Summary

• Protists have nuclear membranes around their DNA. They also have other membrane-bound organelles. • Many protists live in aquatic habitats, and most are motile, or able to move. • Protists have complex life cycles that may include both sexual and asexual reproduction. • Protists get food through ingestion, absorption, or photosynthesis.

Explore More

Use this resource to answer the questions that follow.

• Characteristics of Protists at http://cnx.org/content/m44616/latest/?collection=col11448/1.9.

1. Explain the cell structure characteristics of protists. 2. Explain the metabolic diversity of protists. 3. Explain protist motility. 4. Explain the life cycle diversity of protists.

Explore More Answers

1. Most protists are microscopic and unicellular, but some true multicellular forms exist. A few protists live as colonies that behave in some ways as a group of free-living cells and in other ways as a multicellular organism. Still other protists are composed of enormous, multinucleate, single cells. Single protist cells range in size from less than a micrometer to three meters in length to hectares. Protist cells may be enveloped by animal- like cell membranes or plant-like cell walls. Others are encased in glassy silica-based shells or wound with pellicles of interlocking protein strips. 2. Protists exhibit many forms of nutrition and may be aerobic or anaerobic. Protists that store energy by photosynthesis are characterized by chloroplasts. Other protists are heterotrophic and consume organic materials to obtain nutrition. Amoebas and some other heterotrophic protist species ingest particles by phagocytosis. The vesicle containing the ingested particle fuses with a lysosome, and the food particle is broken down into small molecules that can be used in cellular metabolism. Subtypes of heterotrophs, called saprobes, absorb nutrients from dead organisms or their organic wastes. Some protists can function as mixotrophs, obtaining nutrition by photoautotrophic or heterotrophic routes, depending on whether sunlight or organic nutrients are available. 3. The majority of protists are motile, but different types of protists have evolved varied modes of movement. Some protists have one or more flagella, which they rotate or whip. Others are covered in rows of cilia that they coordinately beat to swim. Still others form pseudopodia anywhere on the cell, anchor the pseudopodia to a substrate, and pull themselves forward. Some protists can move toward or away from a stimulus. Movement toward light is accomplished by coupling their locomotion strategy with a light-sensing organ. 4. Most protists undergo some form of asexual reproduction, such as binary fission, to produce two daughter cells. Some protists exhibit multiple fission and simultaneously divide into many daughter cells. Others

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produce tiny buds that go on to divide and grow to the size of the parental protist. Sexual reproduction, involving meiosis and fertilization, is common among protists, and many protist species can switch from asexual to sexual reproduction when necessary. Protist life cycles range from simple to extremely elaborate. Certain parasitic protists have complicated life cycles and must infect different host species at different developmental stages to complete their life cycle. Some protists are unicellular in the haploid form and multicellular in the diploid form. Other protists have multicellular stages in both haploid and diploid forms, an alternation of generations strategy.

Review

1. Identify three structures that protists use to move. 2. Describe three ways that protists get food. 3. Describe asexual and sexual reproduction in protists.

Review Answers

1. Protists have three types of appendages for movement: ﬂagella, cilia, or pseudopods. 2. Three ways that protists get food. (1) Ingestive protists ingest, or engulf, bacteria and other small particles. They extend their cell wall and cell membrane around the food item, forming a food vacuole. Then enzymes digest the food in the vacuole. (2) Absorptive protists absorb food molecules across their cell membranes. This occurs by diffusion. These protists are important decomposers. (3) Photosynthetic protists use light energy to make food. They are major producers in aquatic ecosystems. 3. Asexual reproduction can occur when single-celled protists reproduce by binary ﬁssion. In a stressful environment, protists may produce tough spores that can withstand harsh conditions. If two protist spores are close together, they can fuse to form a diploid zygote. This is a type of sexual reproduction.

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1.4 Protozoa

• Describe animal-like protists. • Describe the position of protozoa in food chains. • Summarize the classiﬁcation of protozoa.

What’s like an animal, but not an animal? An animal-like protist, or a protozoa. These protists have the ability to move, usually with some sort of cilia or ﬂagella, and must obtain their energy from other sources. But obviously, they are much simpler than animals.

Animal-Like Protists: Protozoa

Animal-like protists are commonly called protozoa (singular, protozoan). Most protozoa consist of a single cell. They are animal-like because they are heterotrophs, and are capable of moving. Although protozoa are not animals, they are thought to be the ancestors of animals.

Ecology of Protozoa

Protozoa generally feed by engulﬁng and digesting other organisms. As consumers, they have various roles in food chains and webs. Some are predators. They prey upon other single-celled organisms, such as bacteria. In fact, protozoa predators keep many bacterial populations under control. Other protozoa are herbivores. They graze on algae. Still others are decomposers. They consume dead organic matter. There are also parasitic protozoa that live in or on living hosts. For example, the protozoan that causes malaria lives inside a human host. Protozoa are also important food sources for many larger organisms, including insects and worms.

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Classiﬁcation of Protozoa

Protozoa can be classiﬁed on the basis of how they move. As shown in Table 1.1, protozoa move in three different ways. Only sporozoa cannot move. Note that this classiﬁcation is based only on differences in movement. It does not represent phylogenetic relationships.

TABLE 1.1: Classiﬁcation of Protozoa Based on Movement

Type of Protozoa How It Moves Example (Genus) Amoeboid pseudopods Amoeba

Ciliate cilia Paramecium

Flagellate ﬂagella Giardia

Sporozoan does not move (as adult) Plasmodium

Summary

• Animal-like protists are called protozoa. Most consist a single cell. • Like animals, protozoa are heterotrophic and capable of moving. • Examples of protozoa include amoebas and paramecia.

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Explore More

Use this resource to answer the questions that follow.

• Protozoa at http://www.edu.pe.ca/southernkings/microprotozoa.htm.

1. What do protozoa feed on? 2. Describe the three general groups of protozoa. 3. Explain movement of amoebas.

Explore More Answers

1. Protozoa mainly feed on bacteria, but they also eat other protozoa,and sometimes fungi. 2. One group is the Ciliates, which are generally the largest protozoa. They have hair-like cilia and they eat the other two types of protozoa as well as bacteria. The second group is the Amoebae. The third group is the Flagellates, which are usually the smallest of the protozoa and have one or several long, whip-like flagella extending from their cells. 3. Amoebas ooze about by extending parts of their cells. Amoebae have fluid cell membranes or coverings that they can stretch out, bend and curve. As the membrane moves outward, the fluid and other parts inside the cell follow, flowing into the new bulge created by the moving membrane.

Review

1. How are protozoa similar to animals? 2. What roles do protozoa play in food chains and webs? 3. What type of protozoa is a Paramecium? 4. What type of protozoa is a Giardia?

Review Answers

1. Protozoa are animal-like because they are heterotrophs, and are capable of moving. 2. As consumers, protozoa have various roles in food chains and webs. Some are predators. They prey upon other single-celled organisms, such as bacteria. Other protozoa are herbivores. They graze on algae. Still others are decomposers. They consume dead organic matter. There are also parasitic protozoa that live in or on living hosts. Protozoa are also important food sources for many larger organisms, including insects and worms. 3.A Paramecium is a ciliate. 4.A Giardia is a ﬂagellate.

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1.5 Algae

• Give an overview of plant-like protists. • Give examples of algae. • Explain why are algae considered plant-like. • Describe the role of algae in the food chain. • Summarize the classiﬁcation of algae. • Explain the life cycles of algae.

Many people, if not most, believe seaweed to be a plant. Is it? Seaweed is actually a plant-like protist, which are also known as algae. The green color is due to what pigment? Algae, like plants, obtain their energy through photosynthesis.

Plant-Like Protists: Algae

Plant-like protists are called algae (singular, alga). They are a large and diverse group. Some algae, the diatoms, are single-celled. Others, such as seaweed, are multicellular (see Figure 1.5). Why are algae considered plant-like? The main reason is that they contain chloroplasts and produce food through photosynthesis. However, they lack many other structures of true plants. For example, algae do not have roots, stems, or leaves. Some algae also differ from plants in being motile. They may move with pseudopods or ﬂagella. Although not plants themselves, algae were probably the ancestors of plants.

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FIGURE 1.5 Diatoms are single-celled algae. Other forms of algae are multicellular.

Ecology of Algae

Algae play signiﬁcant roles as producers in aquatic ecosystems. Microscopic forms live suspended in the water column. They are the main component of phytoplankton. As such, they contribute to the food base of most marine ecosystems. Multicellular seaweeds called kelp may grow as large as trees. They are the food base of ecosystems called kelp forests (see Figure 1.6). Kelp forests are found throughout the ocean in temperate and arctic climates. They are highly productive ecosystems.

FIGURE 1.6 Kelp Forest. This kelp forest supports a large community of many other types of organisms.

Classiﬁcation of Algae

Types of algae include red and green algae, and euglenids, and dinoﬂagellates (see Table 1.2 for examples). Scientists think that red and green algae evolved from endosymbiotic relationships with cyanobacteria. Their chloroplasts have two membranes because the cell membranes of the cyanobacteria became additional plasma membranes of the chloroplasts. Scientists think that euglenids and dinoﬂagellates evolved later, from endosymbiotic relationships with green and red algae. This is why their chloroplasts have three membranes. Differences in the types of chlorophyll in the four types of algae also support the hypothesized evolutionary relationships.

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TABLE 1.2: Types of Algae

Type of Algae Origin of Chloroplast Type of Chloroplast Red algae cyanobacteria two membranes, chlorophyll like the majority of cyanobacteria

Green algae cyanobacteria two membranes, chlorophyll like a minority of cyanobacteria

Euglenids green algae three membranes, chlorophyll like green algae

Dinoﬂagellates red algae three membranes, chlorophyll like red algae

Reproduction of Algae

Algae have varied life cycles. Two examples are shown in Figure 1.7. Both cycles include phases of asexual reproduction (haploid, n) and sexual reproduction (diploid, 2n). Why go to so much trouble to reproduce? Asexual reproduction is fast, but it doesn’t create new genetic variation. Sexual reproduction is more complicated and risky, but it creates new gene combinations. Each strategy may work better under different conditions. Rapid population growth (asexual reproduction) is adaptive when conditions are favorable. Genetic variation (sexual reproduction) helps ensure that some organisms will survive if the environment changes.

KQED: Algae Power

QUEST explores the potential of algae-–once considered nothing more than pond scum—to become the fuel of the future. Entrepreneurs from throughout California are working to create the next generation of biofuels from algae. But will you ever be able to run your car off it? See http://www.kqed.org/quest/television/algae-power for additional information.

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FIGURE 1.7 Life Cycles of Algae: Zygotic Meiosis (A), Gametic Meiosis (B) and Sporic Meiosis (C). In life cycle A (left), diploid (2n) zygotes result from fertilization and then undergo meiosis to produce haploid (n) gametes. The gametes undergo mitosis and produce many additional copies of themselves. How are life cycles B and C different from life cycle A?

MEDIA Click image to the left for use the URL below. URL: http://gamma.ck12.org/ﬂx/render/embeddedobject/453

Summary

• Plant-like protists are called algae. They include single-celled diatoms and multicellular seaweed. • Like plants, algae contain chlorophyll and make food by photosynthesis. • Types of algae include red and green algae, euglenids, and dinoﬂagellates.

Explore More

Use this resource to answer the questions that follow.

• Algae at http://www.edu.pe.ca/southernkings/microalgae.htm.

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1. Where are algae found? 2. How do algae obtain energy? Why is this important for animals? 3. What is special about diatoms? 4. When can certain algae cause harm to other organisms?

Explore More Answers

1. Algae are found in fresh and salt water around the world. They can also grow on rocks and trees and in soil when enough water is available. 2. Most algae are able to make energy from sunlight through photosynthesis. They produce a large amount of the oxygen we breathe. 3. Diatoms have hard shells made out of glass. When they die, these shells sink to the bottom of their watery environments. The shells are used to make various products. 4. Certain kinds of algae can grow in such large numbers that when they suddenly die off en masse, the breaking down of their cells by bacteria destroys the amount of dissolved oxygen in the water, hurting the animals and plants that live there (the negative effects of an algal bloom).

Review

1. Compare and contrast algae and plants. 2. State pros and cons of asexual and sexual reproduction in algae. 3. Explain why dinoﬂagellates and euglenids have chloroplasts with three membranes instead of two.

Review Answers

1. Plant-like protists are called algae. The main reason algae are plant-like is that they contain chloroplasts and produce food through photosynthesis. However, they lack many other structures of true plants. For example, algae do not have roots, stems, or leaves. Some algae also differ from plants in being motile. They may move with pseudopods or ﬂagella. Although not plants themselves, algae were probably the ancestors of plants. 2. Asexual reproduction is fast, but it doesn’t create new genetic variation. Sexual reproduction is more complicated and risky, but it creates new gene combinations. Each strategy may work better under different conditions. Rapid population growth (asexual reproduction) is adaptive when conditions are favorable. Genetic variation (sexual reproduction) helps ensure that some organisms will survive if the environment changes. 3. Scientists think that euglenids and dinoﬂagellates evolved from endosymbiotic relationships with green and red algae, whose chloroplasts have two membranes. This is why their chloroplasts have three membranes.

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1.6 Molds

• Identify types of fungus-like protists. • Give an overview of molds. • Compare slime molds to water molds.

What grows just about anywhere there is decaying material? Mold. This slime mold, shown growing on dead wood, is a fungus-like protist. Though this mold does not have a mouth, essentially it is still “eating” this decaying material.

Fungus-Like Protists: Molds

Fungus-like protists are molds. They are absorptive feeders on decaying organic matter. They resemble fungi, and they reproduce with spores as fungi do. However, in other ways, they are quite different from fungi and more like other protists. For example, they have cell walls made of cellulose, whereas fungi have cell walls made of chitin. Like other protists, they have complicated life cycles with both asexual and sexual reproduction. They are motile during some stages of their life cycle. Two major types of fungus-like protists are slime molds and water molds.

Slime Molds

Slime molds are fungus-like protists commonly found on rotting logs and compost. They move very slowly in search of decaying matter to eat. When food is scarce, individual cells swarm together to form a blob-like mass, like the “dog vomit” slime mold in the Figure 1.8. The mass glides along on its own secretions, engulﬁng decaying organic matter as it moves over it. There are two types of slime molds when it comes to how they swarm: acellular and cellular.

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FIGURE 1.8 “Dog Vomit” Slime Mold. This slime mold looks like its name.

• When acellular slime molds swarm, they fuse together to form a single cell with many nuclei. • When cellular slime molds swarm, they remain as distinct cells.

Cellular slime molds are used as model organisms in molecular biology and genetics. They may be the key to how multicellular organisms evolved. Can you explain why?

Water Molds

Water molds are commonly found in moist soil and surface water. Many are plant pathogens that destroy crops. They infect plants such as grapes, lettuce, corn, and potatoes. Some water molds are parasites of ﬁsh and other aquatic organisms.

Summary

• Fungus-like protists are molds. • Molds are absorptive feeders, found on decaying organic matter. They resemble fungi and reproduce with spores as fungi do. • Examples of fungus-like protists include slime molds and water molds.

Explore More

Use this resource to answer the questions that follow.

• Funguslike Protists at http://jcareywi.tripod.com/id23.html.

1. Give three facts about slime molds. 2. Give two facts about cellular slime molds. 3. Give three facts about acellular slime molds. 4. Give three facts about water molds.

22 www.ck12.org Chapter 1. Protists and Fungi

Explore More Answers

1. Slime molds are found on forest ﬂoors or a backyard compost pile. At one stage in their life cycle, they resemble amoebas, in the other stage, they form moldlike clumps that produce spores. 2. Answers may vary. Cellular slime molds spend most of their lives as free-living cells resembling soil amoebas. When their food supply is gone, they produce spores that can survive harsh conditions. 3. Answers may vary. Acellular slime molds begin their life cycle as an amoeba-like cell. When they aggregate, their cells fuse to produce structures with many nuclei or plasmodia. They can grow as large as several meters in diameter. 4. Answers may vary. Water molds resemble white fuzz growing on the surface of dead ﬁsh in the water. They thrive on dead and decaying organic matter in the water. Some water molds are plant parasites on land.

Review

1. How are fungus-like protists similar to fungi? What is one way they are different? 2. Why might cellular slime molds, but not acellular slime molds, be the key to how multicellular organisms evolved?

Review Answers

1. Fungus-like protists are molds. They are absorptive feeders on decaying organic matter. They resemble fungi, and they reproduce with spores as fungi do. One way they are different is they have cell walls made of cellulose, whereas fungi have cell walls made of chitin. 2. When acellular slime molds swarm, they fuse together to form a single cell with many nuclei. When cellular slime molds swarm, they remain as distinct cells, so they may provide information as to how multicellular organisms evolved.

23 1.7. Protists and Human Disease www.ck12.org

1.7 Protists and Human Disease

• Give examples of diseases caused by protists. • Explain how Trypanosoma and Plasmodium cause disease. • Describe giardiasis.

Can such little creatures make you sick? They sure can. Not all of them, but some of them. And without proper medical treatment, the person may never recover.

Protists and Human Disease

Most protist diseases in humans are caused by animal-like protists, or protozoa. Protozoa make us sick when they become human parasites. Three examples of parasitic protozoa are described below.

Members of the genus Trypanosoma are flagellate protozoa that cause sleeping sickness, which is common in Africa. They also cause Chagas disease, which is common in South America. The parasites are spread by insect vectors. The vector for Chagas disease is shown in Figure 1.9. Trypanosoma parasites enter a person’s blood when the vector bites. Then they spread to other tissues and organs. The diseases may be fatal without medical treatment. The discovery of Chagas disease is unique in the history of medicine. That’s because a single researcher—a Brazilian physician named Carlos Chagas—single-handedly identified and explained the new infectious disease. In the early 1900s, Chagas did careful lab and field studies. He determined the pathogen, vector, host, symptoms, and mode of transmission of the disease that is now named for him.

24 www.ck12.org Chapter 1. Protists and Fungi

FIGURE 1.9 Vector for Chagas Disease. In Chagas disease, the Trypanosoma parasite is spread by an insect commonly called the “kissing bug.” A bite from this bug could be the kiss of death.

Giardia are ﬂagellate protozoa that cause giardiasis. The parasites enter the body through food or water that has been contaminated by feces of infected people or animals. The protozoa attach to the lining of the host’s small intestine, where they prevent the host from fully absorbing nutrients. They may also cause diarrhea, abdominal pain, and fever. A picture of a Giardia protozoan opens this concept.

Plasmodium protozoa cause malaria. The parasites are spread by a mosquito vector. Parasites enter a host’s blood through the bite of an infected mosquito. The parasites infect the host’s red blood cells, causing symptoms such as fever, joint pain, anemia, and fatigue. Malaria is common in tropical and subtropical climates throughout the world (see Figure 1.10). In fact, malaria is one of the most common infectious diseases on the planet. Malaria is also a very serious disease. It kills several million people each year, most of them children. A vaccine to malaria is a possibility. See Vaccine Could Become ’Another Very Powerful Weapon’ to Fight Malaria at http://video.pbs.org/video/2156092045 for additional information.

Summary

• Most protist diseases in humans are caused by protozoa. Protozoa make humans sick when they become human parasites. • Trypanosoma protozoa cause Chagas disease and sleeping sickness. • Giardia protozoa cause giardiasis, and Plasmodium protozoa cause malaria.

Explore More

Use this resource to answer the questions that follow.

• http://www.hippocampus.org/Biology → Non-Majors Biology → Search: Protozoa

1. Can protozoans cause deadly disease in humans? 2. What is Giardia? How does it spread and what are the manifestations of a Giardia infection? 3. What protist causes malaria?

Explore More Answers

1. Yes, protozoans can cause deadly disease in humans.

25 1.7. Protists and Human Disease www.ck12.org

FIGURE 1.10 Worldwide Distribution of Malaria. This map shows where malaria is found. The area is determined by the mosquito vector. The mosquito can live year-round only in the red-shaded areas.

2. Giardia is a ﬂagellate protozoan. It spreads from animals to humans through polluted water, and causes diarrhea and severe stomach cramps. 3. Apicomplexans are responsible for malaria.

Review

1. Describe how the protozoa that cause Chagas disease are spread to human hosts. 2. State why malaria is commonly found only in tropical and subtropical regions of the world. 3. Terri lost her water bottle while hiking in Canada. It was a hot day, so she drank water from a stream to stay hydrated. A few days later, Terri became ill with abdominal pain, fever, and diarrhea. Her doctor thinks she has a protozoan infection. Which type of protozoa do you think is most likely responsible for Terri’s illness? How do you think Terri became infected?

Review Answers

1. The protozoa that cause Chagas disease are spread to human hosts by insect vectors. Trypanosoma parasites enter a person’s blood when the vector bites. Then they spread to other tissues and organs. 2. The malaria parasites are spread by a mosquito vector. The mosquito can live year-round only in tropical and subtropical regions of the world. 3. Terri probably has giardiasis. The Giardia parasites enter the body through food or water that has been contaminated by feces of infected people or animals. The protozoa attach to the lining of the host’s small intestine, where they prevent the host from fully absorbing nutrients. They may also cause diarrhea, abdominal pain, and fever. Terri was infected from drinking stream water.

26 www.ck12.org Chapter 1. Protists and Fungi

1.8 Fungi

• Describe the main characteristics of fungi. • Give examples of fungi. • Describe habitats of fungi.

What exactly is a fungus? They’re not animals or plants, and deﬁnitely not protists. So they cannot photosynthesize or eat. And they are much more than mushrooms.

Characteristics of Fungi

Do you see the organisms growing on the bread in the Figure 1.11? They belong to the Kingdom Fungi. Molds growing on foods are some of the most common fungi in our everyday lives. These organisms may seem useless, gross, and costly. But fungi play very important roles in almost every terrestrial ecosystem on Earth. Fungi (singular, fungus) are a kingdom in the domain Eukarya. The fungi kingdom may contain more than a million species, but fewer than 100,000 have been identiﬁed. As shown in the Figure 1.12, fungi include mushrooms and yeasts in addition to molds. Most fungi are multicellular, but some exist as single cells. Single-celled fungi are known as yeasts. Fungi spend most of their life cycle in the haploid state. They form diploid cells only during sexual reproduction. Like the cells of protists and plants, the cells of fungi have cell walls. But fungi are unique in having cell walls made of chitin instead of cellulose. Chitin is a tough carbohydrate that also makes up the exoskeleton (outer skeleton) of insects and related organisms.

27 1.8. Fungi www.ck12.org

FIGURE 1.11 The mold growing on this bread is a common fungus.

FIGURE 1.12 Several examples of fungi are pictured here.

Habitats of Fungi

Fungi are found all around the world, and grow in a wide range of habitats, including deserts. Most grow in terrestrial environments, but several species live only in aquatic habitats. Most fungi live in soil or dead matter, and in symbiotic relationships with plants, animals, or other fungi. Fungi, along with bacteria that are found in soil, are the primary decomposers of organic matter in terrestrial ecosystems. The decomposition of dead organisms returns nutrient to the soil, and the environment.

Summary

• Fungi are a kingdom in the domain Eukarya that includes molds, mushrooms, and yeasts. • Most fungi are multicellular. They are unique in having cell walls made of chitin. • Most fungi live on dead matter or soil. Some live in aquatic habitats. Many are involved in symbiotic relationships.

28 www.ck12.org Chapter 1. Protists and Fungi

Explore More

Explore More I

Use this resource to answer the questions that follow.

• Fungi at http://www.edu.pe.ca/southernkings/microfungi.htm.

1. Give examples of single-celled and multi celled fungi. 2. Where do fungi live? 3. How do fungi move? Explain your answer. 4. How do fungi obtain nutrients?

Explore More II • The Fungi Kingdom at http://www.wisc-online.com/Objects/ViewObject.aspx?ID=BIO304.

Explore More Answers

Explore More I 1. Fungi include single-celled living things that exist individually, such as yeast, and multicellular clusters, such as molds or mushrooms 2. Fungi live in the soil and on your body, in your house and on plants and animals, in freshwater and seawater. 3. Fungi do not move, but they can spread either by forming reproductive spores that are carried on wind and rain or by growing and extending their hyphae. 4. Fungi absorb nutrients from living or dead organic matter that they grow on. They absorb simple, easily dissolved nutrients, such as sugars, through their cell walls. They give off special digestive enzymes to break down complex nutrients into simpler forms that they can absorb.

Review

1. What are fungi? Give two examples of fungi. 2. Explain the signiﬁcance of the chitin cell wall of fungi. 3. List two habitats where fungi live.

Review Answers

1. Fungi are a kingdom in the domain Eukarya. Fungi include mushrooms and yeasts in addition to molds. 2. Fungi are unique in having cell walls made of chitin instead of cellulose. 3. Most fungi live in soil or dead matter, and in terrestrial habitats, including deserts.

29 1.9. Fungi Structure www.ck12.org

1.9 Fungi Structure

• Outline the structure of fungi. • Deﬁne hyphae and mycelium. • Explain the signiﬁcance of a fruiting body.

Is the structure important? Of course. Though mushrooms may be the most common type of fungus, fungi also include rusts, smuts, puffballs, trufﬂes, morels, molds, and yeasts, as well as many less well-known organisms. And, except for yeast cells, they all have similar structures, which are usually hidden deep within their food source.

Structure of Fungi

Except for yeasts, which grow as single cells, most fungi grow as thread-like filaments, like those shown in Figure 1.13. The filaments are called hyphae (singular, hypha). Each hypha consists of one or more cells surrounded by a tubular cell wall. A mass of hyphae make up the body of a fungus, which is called a mycelium (plural, mycelia). The hyphae of most fungi are divided into cells by internal walls called septa (singular, septum). Septa usually have little pores that are large enough to allow ribosomes, mitochondria and sometimes nuclei to flow among cells. Hyphae that are divided into cells are called septate hyphae. However, the hyphae of some fungi are not separated by septa. Hyphae without septae are called coenocytic hyphae. Coenocytic hyphae are big, multinucleated cells. A mycelium may range in size from microscopic to very large. In fact, one of the largest living organisms on Earth is the mycelium of a single fungus. A small part of a similar fungus is pictured in Figure 1.14. The giant fungus covers 8.9 square kilometers (3.4 square miles) in an Oregon forest. That’s about the size of a small city. The fungus didn’t grow that large overnight. It’s estimated to be 2,400 years old, and it’s still growing!

30 www.ck12.org Chapter 1. Protists and Fungi

FIGURE 1.13 These branches are hyphae, or ﬁlaments, of a mold called Penicillium.

FIGURE 1.14 The fungus shown here has been dubbed the “humongous fungus” because it covers such a large area.

Fruiting Bodies

Some fungi become noticeable only when producing spores (fruiting), either as mushrooms or molds. For example, you can see the fruiting bodies of the Armillaria fungus in the Figure 1.14, but the large “body” of the fungus, the mycelium, is hidden underground. This fruiting body, known as the sporocarp, is a multicellular structure on which spore-producing structures form. The fruiting body is part of the sexual phase of a fungal life cycle. The rest of the life cycle is characterized by the growth of mycelia.

Dimorphic Fungi

Some fungi take on different shapes, depending on their environmental conditions. These fungi are called dimorphic fungi, because they have “two forms.” For example, the fungus Histoplasma capsulatum, which causes the disease histoplasmosis, is thermally dimorphic; it has two forms that are dependent on temperature. In temperatures of about

31 1.9. Fungi Structure www.ck12.org

25°C, it grows as a brownish mycelium, and looks like a mass of threads. At body temperature (37°C in humans), it grows as single, round yeast cells.

Summary

• Most fungi grow as thread-like ﬁlaments called hyphae. • A mass of hyphae make up the body of a fungus, called a mycelium.

Explore More

Use this resource to answer the questions that follow.

• Fungal Cell Structure at http://www.aber.ac.uk/fungi/fungi/studying/structure.htm.

1. How do fungal cells differ from plant cells? 2. Describe the typical fungal structure. 3. What is the apical vesicular complex? 4. What is the role of the cell wall in fungal cells? 5. What is a septum?

Explore More Answers

1. Unlike plant cells, the cell wall of fungal cells lacks cellulose (in true fungi) and there are no chloroplasts. 2. The typical fungal structure is that of a colony of cells strung together in a hypha. 3. The apical vesicular complex is found at the actively growing hyphal tip, and consists of a mass of vesicles surrounding the Spitzenkörper (the organizing center for hyphal growth and morphogenesis). 4. The cell wall prevents the hyhae from bursting due to osmosis. 5. A septum can be thought of as a valve between adjacent hyphal cells to prevent the accidental loss of cell organelles between hyphal compartments.

Review

1. Describe the general structure of multicellular fungi. 2. What is a fruiting body? 3. Relate the structures of hyphae, mycelia, and fruiting bodies to one another.

Review Answers

1. Most fungi grow as thread-like hyphae. Each hypha consists of one or more cells surrounded by a tubular cell wall. A mass of hyphae make up the body of a fungus or mycelium. The hyphae of most fungi are divided into cells by internal septa. 2. The fruiting body, the sporocarp, is a multicellular structure on which spore-producing structures form. 3. A mass of hyphae make up the body of a fungus, which is the mycelium. These structures are usually hidden from view. The fruiting body, which is part of the sexual phase of a fungal life cycle, is visible.

32 www.ck12.org Chapter 1. Protists and Fungi

1.10 How Fungi Eat

• Relate the structure of fungi to how they obtain nutrients. • Describe mycorrhiza.

So what do fungi "eat"? Just about anything. From dead plants to rotting fruit. Shown here are fungi sprouting from dead material in the woods. Fungi perform an essential role in the decomposition of organic matter and have fundamental roles in nutrient cycling and exchange.

Nutrition

Fungi get their nutrition by absorbing organic compounds from the environment. Fungi are heterotrophic: they rely solely on carbon obtained from other organisms for their metabolism and nutrition. Fungi have evolved in a way that allows many of them to use a large variety of organic substrates for growth, including simple compounds such as nitrate, ammonia, acetate, or ethanol. Their mode of nutrition deﬁnes the role of fungi in their environment. Fungi obtain nutrients in three different ways:

1. They decompose dead organic matter. A saprotroph is an organism that obtains its nutrients from non-living organic matter, usually dead and decaying plant or animal matter, by absorbing soluble organic compounds. Saprotrophic fungi play very important roles as recyclers in ecosystem energy ﬂow and biogeochemical cycles. Saprophytic fungi, such as shiitake (Lentinula edodes) and oyster mushrooms (Pleurotus ostreatus), decompose dead plant and animal tissue by releasing enzymes from hyphal tips. In this way they recycle organic materials back into the surrounding environment. Because of these abilities, fungi are the primary decomposers in forests (see Figure 1.15).

33 1.10. How Fungi Eat www.ck12.org

2. They feed on living hosts. As parasites, fungi live in or on other organisms and get their nutrients from their host. Parasitic fungi use enzymes to break down living tissue, which may causes illness in the host. Disease- causing fungi are parasitic. Recall that parasitism is a type of symbiotic relationship between organisms of different species in which one, the parasite, beneﬁts from a close association with the other, the host, which is harmed. 3. They live mutualistically with other organisms. Mutualistic fungi live harmlessly with other living organisms. Recall that mutualism is an interaction between individuals of two different species, in which both individuals beneﬁt.

Both parasitism and mutualism are classiﬁed as symbiotic relationships, but they are discussed separately here because of the different effect on the host.

FIGURE 1.15 Forest Decomposers. These forest mushrooms may look fragile, but they do a powerful job. They decompose dead wood and other tough plant material.

Fungal hyphae are adapted to efﬁcient absorption of nutrients from their environments, because hyphae have high surface area-to-volume ratios. These adaptations are also complemented by the release of hydrolytic enzymes that break down large organic molecules such as polysaccharides, proteins, and lipids into smaller molecules. These molecules are then absorbed as nutrients into the fungal cells. One enzyme that is secreted by fungi is cellulase, which breaks down the polysaccharide cellulose. Cellulose is a major component of plant cell walls. In some cases, fungi have developed specialized structures for nutrient uptake from living hosts, which penetrate into the host cells for nutrient uptake by the fungus.

Mycorrhiza

A mycorrhiza (Greek for "fungus roots") is a symbiotic association between a fungus and the roots of a plant. In a mycorrhizal association, the fungus may colonize the roots of a host plant by either growing directly into the root cells, or by growing around the root cells. This association provides the fungus with relatively constant and direct access to glucose, which the plant produces by photosynthesis. The mycelia of the fungi increase the surface area of the plant’s root system. The larger surface area improves water and mineral nutrient absorption from the soil.

Summary

• Fungi are heterotrophic. They get their nutrition by absorbing organic compounds from the environment.

34 www.ck12.org Chapter 1. Protists and Fungi

FIGURE 1.16 Fungal mycelia. Fungi absorb nutrients from the environment through mycelia. The branching mycelia have a high surface-area-to-volume ratio which allows for efﬁcient absorption of nutrients. Some fungi digest nutrients by releasing enzymes into the environment.

• Fungi, along with bacteria that are found in soil, are the primary decomposers of organic matter in terrestrial ecosystems.

Explore More

Use this resource to answer the questions that follow.

• Fungus Nutrition at http://www.britannica.com/EBchecked/topic/222357/fungus/57967/Nutrition.

1. Describe how fungi obtain food. 2. How does food enter a fungus? 3. Why are saprobic fungi important? 4. Where do fungi obtain their carbon? 5. Where do fungi obtain their nitrogen?

Explore More Answers

1. Fungi secure food through the action of enzymes secreted into the surface on which they are growing. The enzymes digest the food, which then is absorbed directly through the fungal hyphal walls. 2. Food is absorbed into a fungus. Food must be in solution in order to enter the hyphae, and the entire mycelial surface of a fungus is capable of absorbing materials dissolved in water. 3. Together with bacteria, saprobic fungi are to a large extent responsible for the decomposition of organic matter. 4. Carbon is supplied to fungi in the form of sugars or starch. The majority of fungi thrive on such sugars as glucose, fructose, mannose, maltose, and, to a lesser extent, sucrose. 5. Many fungi obtain their nitrogen from decomposition products of proteins, such as proteoses, peptones, and amino acids. Ammonium compounds and nitrates also serve as nutrients for many species.

Review

1. Describe how fungi obtain nutrients.

35 1.10. How Fungi Eat www.ck12.org

2. Explain the role of saprotrophic fungi? Give an example of this role. 3. What is a mycorrhiza? What are the advantages of a mycorrhiza?

Review Answers

1. Fungi obtain nutrients in three different ways: they decompose dead organic matter, they feed on living hosts, or they live mutualistically with other organisms. 2. Saprotrophic fungi play very important roles as recyclers in ecosystem energy ﬂow and biogeochemical cycles. Saprophytic fungi, such as shiitake and oyster mushrooms, decompose dead plant and animal tissue by releasing enzymes from hyphal tips. In this way they recycle organic materials back into the surrounding environment. Because of these abilities, fungi are the primary decomposers in forests 3. A mycorrhiza is a symbiotic association between a fungus and the roots of a plant. In a mycorrhizal association, the fungus may colonize the roots of a host plant by either growing directly into the root cells, or by growing around the root cells. This association provides the fungus with relatively constant and direct access to glucose, which the plant produces by photosynthesis. The mycelia of the fungi increase the surface area of the plant’s root system. The larger surface area improves water and mineral nutrient absorption from the soil.

36 www.ck12.org Chapter 1. Protists and Fungi

1.11 Fungi Reproduction

• Explain fungi reproduction. • Describe a fungal spore. • Distinguish between a spore and a zygospore.

How do fungi reproduce? Sexually or asexually? How about both? That would suggest that fungi can produce both diploid and haploid cells, which they can. Shown above are fungi mycelia and haploid spores. Spores allow fungi to reproduce through unfavorable conditions.

Reproduction of Fungi

The majority of fungi can reproduce both asexually and sexually. This allows them to adjust to conditions in the environment. They can spread quickly through asexual reproduction when conditions are stable. They can increase their genetic variation through sexual reproduction when conditions are changing and variation may help them survive.

Asexual Reproduction

Almost all fungi reproduce asexually by producing spores. A fungal spore is a haploid cell produced by mitosis from a haploid parent cell. It is genetically identical to the parent cell. Fungal spores can develop into new haploid individuals without being fertilized. Spores may be dispersed by moving water, wind, or other organisms. Some fungi even have “cannons” that “shoot” the spores far from the parent organism. This helps to ensure that the offspring will not have to compete with the

37 1.11. Fungi Reproduction www.ck12.org

parent for space or other resources. You are probably familiar with puffballs, like the one in Figure 1.17. They release a cloud of spores when knocked or stepped on. Wherever the spores happen to land, they do not germinate until conditions are favorable for growth. Then they develop into new hyphae.

FIGURE 1.17 Puffballs release spores when disturbed.

Yeasts do not produce spores. Instead, they reproduce asexually by budding. Budding is the pinching off of an offspring from the parent cell. The offspring cell is genetically identical to the parent. Budding in yeast is pictured in Figure 1.18.

FIGURE 1.18 Yeast reproduce asexually by budding.

38 www.ck12.org Chapter 1. Protists and Fungi

Sexual Reproduction

Sexual reproduction also occurs in virtually all fungi. This involves mating between two haploid hyphae. During mating, two haploid parent cells fuse, forming a diploid spore called a zygospore. The zygospore is genetically different from the parents. After the zygospore germinates, it can undergo meiosis, forming haploid cells that develop into new hyphae.

Summary

• The majority of fungi can reproduce both asexually and sexually. This allows them to adjust to conditions in the environment. • Yeast reproduce asexually by budding. Other fungi reproduce asexually by producing spores. • Sexual reproduction occurs when spores from two parents fuse and form a zygospore.

Explore More

Use this resource to answer the questions that follow.

• Reproduction at https://www.boundless.com/biology/textbooks/boundless-biology-textbook/fungi-24/characte ristics-of-fungi-149/reproduction-591-11810/.

1. How do fungi reproduce asexually? 2. Describe budding. 3. How are asexual spores similar to the parent? 4. What is the advantage of sexual reproduction? 5. Describe plasmogamy. 6. What happens during and immediately after karyogamy?

Explore More Answers

1. Fungi can reproduce asexually by fragmentation, budding, or producing spores. 2. During budding, a bulge forms on the side of the cell, and the bud ultimately detaches after the nucleus divides mitotically. 3. Asexual spores are genetically identical to the parent and may be released either outside or within a spo- rangium. 4. Sexual reproduction introduces genetic variation into a population of fungi. 5. During plasmogamy, two haploid cells fuse, leading to a dikaryotic stage where two haploid nuclei coexist in a single cell. 6. During karyogamy, the haploid nuclei fuse to form a diploid zygote nucleus. Finally, meiosis takes place in the gametangia organs, in which gametes of different mating types are generated. At this stage, spores are disseminated into the environment.

Review

1. Explain the advantages of fungal spores. 2. Identify ways that fungal spores may be dispersed. 3. Compare and contrast a fungal spore and zygospore.

39 1.11. Fungi Reproduction www.ck12.org

Review Answers

1. A fungal spore is a haploid cell produced by mitosis from a haploid parent cell. It is genetically identical to the parent cell. Fungal spores can develop into new haploid individuals without being fertilized. 2. Spores may be dispersed by moving water, wind, or other organisms. Some fungi even have “cannons” that “shoot” the spores far from the parent organism. 3. During mating, two haploid parent cells fuse, forming a diploid zygospore. The zygospore is genetically different from the parents. A fungal spore is a haploid cell produced by mitosis from a haploid parent cell. It is genetically identical to the parent cell. Zygospores from from sexual reproduction. Spores are used in asexual reproduction.

40 www.ck12.org Chapter 1. Protists and Fungi

1.12 Fungi Evolution

• Summarize the evolution of fungi.

What were early fungi like? Early fungi probably lived in water. And they were most likely single-celled organisms. Maybe they lived on dead and decaying material. Obviously, at least in overall size and structure, a mold is very different than a mushroom. Could early fungi have been similar to a mold?

Evolution of Fungi

DNA evidence suggests that almost all fungi have a single common ancestor. The earliest fungi may have evolved about 600 million years ago or even earlier. They were probably aquatic organisms with a ﬂagellum. Fungi ﬁrst colonized the land at least 460 million years ago, around the same time as plants. Fossils of terrestrial fungi date back almost 400 million years (see Figure 1.19). Starting about 250 million years ago, the fossil record shows fungi were abundant in many places. They may have been the dominant life forms on Earth at that time.

41 1.12. Fungi Evolution www.ck12.org

FIGURE 1.19 This rock contains fossilized fungi. The fungi lived 396 million years ago in what is now Scotland. They were preserved when they were covered with lava from a volcano. The lava cooled and hardened into rock.

Summary

• Almost all fungi have a single common ancestor. • The earliest fungi may have evolved about 600 million years ago. • Fungi colonized land at least 460 million years ago. • By 250 million years ago, they may have been the dominant life forms on Earth.

Explore More

Use this resource to answer the questions that follow.

• Fungi Evolution at http://faculty.college-prep.org/~bernie/sciproject/project/Kingdoms/Fungi5/Fungi_Evoluti on.htm.

1. When and where did fungi evolve? 2. Why are fungi considered distinct from plants? What is one main difference? 3. What role did plants play in fungal evolution?

Explore More Answers

1. Fungi evolved around 900 million years ago, developing in aquatic environments, originally from eukaryotic, single-celled protists. 2. Plants and fungi share no general homologous structures, other than those carried over from their protist days. Whereas plants are autotrophic, fungi are always heterotrophic; they depend on the presence of organic material. 3. Plants did play a role in fungal evolution by carrying them onto land, where plants and fungi continue to share a symbiotic relationship.

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Review

1. Summarize the evolution of fungi.

Review Answers

1. DNA evidence suggests that almost all fungi have a single common ancestor. The earliest fungi, which were probable aquatic organisms with a ﬂagellum, may have evolved about 600 million years ago or even earlier. Fungi ﬁrst colonized the land at least 460 million years ago, around the same time as plants. Starting about 250 million years ago, the fossil record shows fungi were abundant in many places. They may have been the dominant life forms on Earth at that time.

43 1.13. Fungi Classiﬁcation www.ck12.org

1.13 Fungi Classiﬁcation

• Give an overview of the classiﬁcation of fungi. • Give examples from the common fungi phyla.

What type of fungus is this? Obviously a mold. But what type of mold? There are thousands of known species of molds. How are they classiﬁed?

Classiﬁcation of Fungi

For a long time, scientists considered fungi to be members of the plant kingdom because they have obvious similarities with plants. Both fungi and plants are immobile, have cell walls, and grow in soil. Some fungi, such as lichens, even look like plants (see Figure 1.20).

The Kingdom Fungi

Today, fungi are no longer classiﬁed as plants. We now know that they have unique physical, chemical, and genetic traits that set them apart from plants and other eukaryotes. For example, the cell walls of fungi are made of chitin, not cellulose. Also, fungi absorb nutrients from other organisms, whereas plants make their own food. These are just a few of the reasons fungi are now placed in their own kingdom.

44 www.ck12.org Chapter 1. Protists and Fungi

FIGURE 1.20 Moss (Plant) and Lichen Growing on Tree Bark. Both fungi and moss are growing on this tree. Can you tell them apart?

Fungal Phyla

Classification of fungi below the level of the kingdom is controversial. There is no single, widely-accepted system of fungal classification. Most classifications include several phyla (the next major taxon below the kingdom). Three of the most common phyla are compared in Table 1.3.

TABLE 1.3: Three Common Phyla of Fungi

Phylum Description Example Zygomycota mainly terrestrial, live in soil and black bread mold compost and on foods such as bread

Basidiomycota have many different shapes, consid- button mushrooms erable variation exists even within species

Ascomycota found in all terrestrial ecosystems baker’s yeast world-wide, even in Antarctica, often involved in symbiotic relationships

Summary

• Fungi used to be classiﬁed as plants. Now, they are known to have unique traits that set them apart from plants.45 For example, fungal cell walls contain chitin, not cellulose, and fungi absorb food rather than make their own. • Below the level of the kingdom, classiﬁcation of fungi is controversial.

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Use this resource to answer the questions that follow.

• Introduction to the Fungi at http://www.botany.hawaii.edu/faculty/wong/Bot201/Myxomycota/Introduction .htm.

1. What is mycology? 2. Give examples of the following fungi: a. Ascomycota b. Agaricales c. Gasteromycetes d. Uredinales e. Ustilaginales 1.13. Fungi Classiﬁcation www.ck12.org

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1. Mycology is the part of botany that studies fungi a. Yeast are ascomycetes. b. mushrooms c. puff balls d. rusts e. smuts

Review

1. State why fungi were once classiﬁed as plants. 2. Explain the signiﬁcance of the chitin cell wall of fungi. 3. Mushrooms belong to what phylum of fungi?

Review Answers

1. For a long time, scientists considered fungi to be members of the plant kingdom because they have obvious similarities with plants. Both fungi and plants are immobile, have cell walls, and grow in soil. Some fungi even look like plants. 2. The cell walls of fungi are made of chitin, not cellulose. This is one of the reasons fungi are now placed in their own kingdom. 3. Mushrooms are members of the phylum Basidiomycota.

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1.14 Symbiotic Relationships of Fungi

• Deﬁne parasitism and mutualism. • Identify symbiotic relationships of fungi. • Distinguish between a mycorrhiza and a lichen.

Do all fungi feed only on dead organisms? Not all. This fungus is a lichen, providing nutrients to the tree. The lichen gets sugars from the plant. Both beneﬁt from this relationship.

Symbiotic Relationships of Fungi

Not all fungi feed on dead organisms. Many are involved in symbiotic relationships, including parasitism and mutualism.

Fungi as Parasites

In a parasitic relationship, the parasite beneﬁts while the host is harmed. Parasitic fungi live in or on other organisms and get their nutrients from them. Fungi have special structures for penetrating a host. They also produce enzymes that break down the host’s tissues. Parasitic fungi often cause illness and may eventually kill their host. They are the major cause of disease in agricultural plants. Fungi also parasitize animals, such as the insect pictured in Figure 1.21. Fungi even parasitize humans. Did you ever have athelete’s foot? If so, you were the host of a parasitic fungus.

47 1.14. Symbiotic Relationships of Fungi www.ck12.org

FIGURE 1.21 Parasitic Fungus and Insect Host. The white parasitic fungus named Cordyceps is shown here growing on its host—a dark brown moth.

Mutualism in Fungi

Fungi have several mutualistic relationships with other organisms. In mutualism, both organisms beneﬁt from the relationship. Two common mutualistic relationships involving fungi are mycorrhiza and lichen.

•A mycorrhiza is a mutualistic relationship between a fungus and a plant. The fungus grows in or on the plant roots. The fungus benefits from the easy access to food made by the plant. The plant benefits because the fungus puts out mycelia that help absorb water and nutrients. Scientists think that a symbiotic relationship such as this may have allowed plants to first colonize the land. •A lichen is an organism that results from a mutualistic relationship between a fungus and a photosynthetic organism. The other organism is usually a cyanobacterium or green alga. The fungus grows around the bacterial or algal cells. The fungus benefits from the constant supply of food produced by the photosynthesizer. The photosynthesizer benefits from the water and nutrients absorbed by the fungus. Figure 1.22 shows lichen growing on a rock.

Some fungi have mutualistic relationships with insects. For example:

• Leafcutter ants grow fungi on beds of leaves in their nests. The fungi get a protected place to live. The ants feed the fungi to their larvae. • Ambrosia beetles bore holes in tree bark and “plant” fungal spores in the holes. The holes in the bark give the fungi an ideal place to grow. The beetles harvest fungi from their “garden.”

Summary

• Many fungi are involved in symbiotic relationships. • Some fungi are parasites. They are specialized to penetrate a host and break down the host’s tissues. Parasitic fungi often cause illness and may eventually kill their host. • Two common mutualistic relationships involving fungi are mycorrhiza (fungi and plant roots) and lichen (fungi and either cyanobacteria or green algae). • Some fungi also have mutualistic relationships with insects.

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FIGURE 1.22 Lichen Growing on Rock. Unlike plants, lichen can grow on bare rocks because they don’t have roots. That’s why lichens are often pioneer species in primary eco- logical succession. How do lichen get water and nutrients without roots?

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Use this resource to answer the questions that follow.

• Symbiotic Fungi at http://www.clarku.edu/faculty/dhibbett/tftol/content/3folder/symbiotic.html.

1. What is symbioses? 2. Give an example of a fungal parasite. 3. Why are many fungi involved in mutualism? 4. What are lichen and mycorrhizae? 5. Give an example of fungal commensalism.

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1. Symbioses are intimate associations involving two or more species. 2. Answers may vary. Asterophora parasitica is a mushroom that grows on mushrooms. Hypomyces lactiﬂuorum is a parasite of ﬂeshy mushrooms. The parasitized mushrooms are edible and are sold as "lobster mushrooms". 3. Many fungal mutualisms are driven by the ability of the fungus to decompose organic substrates that are inaccessible to its host. 4. Lichens are symbioses involving fungi and unicellular algae. Mycorrhizae are symbioses involving fungi and the roots of plants. 5. Laboulbeniales are symbionts that occur on the exoskeleton of insects. Trichomycetes live in the hindgut of aquatic arthropods.

Review

1. How signiﬁcant are fungi as plant parasites?

49 1.14. Symbiotic Relationships of Fungi www.ck12.org

2. Describe an example of a mutualistic relationship between fungi and insects. 3. Assume that you notice a fungus growing on a plant. What possible relationships might exist between the fungus and the plant? What type of evidence might help you identify which is the correct relationship? 4. Compare and contrast mycorrhiza and lichen.

Review Answers

1. Parasitic fungi are the major cause of disease in agricultural plants, making them very significant. 2. (1) Leafcutter ants grow fungi on beds of leaves in their nests. The fungi get a protected place to live. The ants feed the fungi to their larvae. (2) Ambrosia beetles bore holes in tree bark and “plant” fungal spores in the holes. The holes in the bark give the fungi an ideal place to grow. The beetles harvest fungi from their “garden.” 3. The relationship between the fungus and the plant may be parasitic or mutualistic. If the plant is harmed, then the relationship is parasitic. 4. A mycorrhiza is a mutualistic relationship between a fungus and a plant. The fungus grows in or on the plant roots. The fungus benefits from the easy access to food made by the plant. The plant benefits because the fungus puts out mycelia that help absorb water and nutrients. A lichen is an organism that results from a mutualistic relationship between a fungus and a photosynthetic organism. The other organism is usually a cyanobacterium or green alga. The fungus grows around the bacterial or algal cells. The fungus benefits from the constant supply of food produced by the photosynthesizer. The photosynthesizer benefits from the water and nutrients absorbed by the fungus.

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1.15 Human Uses of Fungi

• List human uses of fungi.

What’s growing on this lemon? Would you believe penicillin? Penicillin is a mold, which of course is a fungus, one that has helped millions, if not billions, of people.

Human Uses of Fungi

Whenever you eat pizza, you eat fungi, even if you don’t like your pizza with mushrooms. That’s because pizza dough contains yeast. Do you know other foods that are made with fungi?

Fungi for Food

Humans have collected and grown mushrooms for food for thousands of years. Figure 1.23 shows some of the many types of mushrooms that people eat. Yeasts are used in bread baking and brewing alcoholic beverages. Other fungi are used in fermenting a wide variety of foods, including soy sauce, tempeh, and cheeses. Blue cheese has its distinctive appearance and ﬂavor because of the fungus growing though it (see Figure 1.24).

Fungi for Pest Control

Harmless fungi can be used to control pathogenic bacteria and insect pests on crops. Fungi compete with bacteria for nutrients and space, and they parasitize insects that eat plants. Fungi reduce the need for pesticides and other toxic chemicals.

51 1.15. Human Uses of Fungi www.ck12.org

FIGURE 1.23 These are just a few of the many species of edible mushrooms consumed by humans.

FIGURE 1.24 Blue Cheese. The dark blue strands run- ning through this cheese are a fungus. In fact, this cheese is moldy! The fungus is Penicillium roqueforti, a type of mold.

Other Uses of Fungi

Fungi are useful for many other reasons.

• They are a major source of citric acid (vitamin C). • They produce antibiotics such as penicillin, which has saved countless lives. • They can be genetically engineered to produce insulin and other human hormones. • They are model research organisms. To see how one lab is using yeast in cancer research, watch the video, Yeast Unleashed, at this link: http://college.usc.edu/news/stories/727/yeast-unleashed/.

Summary

• Humans use fungi for many purposes, including as food or in the preparation of food.

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• Humans also use fungi for pest control. • In addition, fungi can be used to produce citric acid, antibiotics, and human hormones. • Fungi are model research organisms as well.

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Use this resource to answer the questions that follow.

• http://www.hippocampus.org/Biology → Non-Majors Biology → Search: Fungi and People

1. How are fungi being used to help human health? 2. Describe how fungi are used as food. 3. Describe commercial uses of fungi.

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1. Drugs derived from fungi are used to save lives. Penicillin and other antibiotics are fungal components that affect bacteria but do not harm animals. Fungal compounds are being used to fight nervous system disorders and treat migraines, lower blood pressure, prevent strokes and heart attacks, and reduce rejection of transplanted organs. 2. Humans eat mushroom, morel, and truffle species. Fungi are used in the production of cheese and tofu. Yeasts are used in bread baking, as well as wine and beer production. 3. Commercially, yeasts and other fungi are used to clean up spills and agricultural and industrial wastes. Fungi increase the efficiency of ethanol fuel production. Pigments from lichens are used as natural dyes, and fungi can be used to control microbial and insect pests.

Review

1. Describe two ways that humans use fungi. 2. How are fungi used to control pests?

Review Answers

1. Fungi is used for food, pest control, medicine, and in research. 2. Harmless fungi can be used to control pathogenic bacteria and insect pests on crops. Fungi compete with bacteria for nutrients and space, and they parasitize insects that eat plants.

53 1.16. Fungi and Human Disease www.ck12.org

1.16 Fungi and Human Disease

• Identify ways fungi can make humans sick. • Identify diseases caused by fungi.

Would you eat these mushrooms? I would not recommend it. But certain red mushrooms, Ganoderma Lucidum, have been found to be good for you. Red Mushrooms comprise a family of more than 200 mushroom species, which are good for our health. Of these, 6 species have a particularly high therapeutic effect.

Fungi and Human Disease

Fungi cause human illness in three different ways: poisonings, parasitic infections, and allergic reactions. Science on the SPOT: Fungus Fair explores some of these dangerous but also tasty and weirdly wonderful fungi. See http ://science.kqed.org/quest/video/science-on-the-spot-fungus-fair/ for details.

Fungal Poisoning

Many fungi protect themselves from parasites and predators by producing toxic chemicals. If people eat toxic fungi, they may experience digestive problems, hallucinations, organ failure, and even death. Most cases of mushroom poisoning are due to mistaken identity. That’s because many toxic mushrooms look very similar to safe, edible mushrooms. An example is shown in Figure 1.25.

Fungal Parasites

Some fungi cause disease when they become human parasites. Two examples are fungi in the genera Candida and Trichophyton.

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FIGURE 1.25 Poisonous or Edible? The destroying angel mushroom on the left causes liver and kidney failure. The puffball mushroom on the right is tasty and harmless. Do you think you could tell these two species of mushrooms apart?

• Candida are yeast that cause candidiasis, commonly called a “yeast infection.” The yeast can infect the mouth or the vagina. If yeast enter the blood, they cause a potentially life threatening illness. However, this is rare, except in people with a depressed immune system. • Trichophyton are fungi that cause ringworm. This is a skin infection characterized by a ring-shaped rash. The rash may occur on the arms, legs, head, neck, or trunk. The same fungi cause athlete’s foot when they infect the skin between the toes. Athlete’s foot is the second most common skin disease in the U.S.

Figure 1.26 shows signs of these two infections.

Fungal Allergies

Mold allergies are very common. They are caused by airborne mold spores. When the spores enter the respiratory tract, the immune system responds to them as though they were harmful microbes. Symptoms may include sneezing, coughing, and difficulty breathing. The symptoms are likely to be more severe in people with asthma or other respiratory diseases. Long-term exposure to mold spores may also weaken the immune system. Molds grow indoors as well as out. Indoors, they grow in showers, basements, and other damp places. Homes damaged in floods and hurricanes may have mold growing just about everywhere (see Figure 1.27). Indoor mold may cause more health problems than outdoor mold because of the closed, confined space. Most people also spend more time indoors than out.

Summary

• Fungi cause three different types of human illness: poisonings, parasitic infections, and allergies. • Many poisonous mushrooms are eaten by mistake because they look like edible mushrooms. • Parasitic yeasts cause candidiasis, ringworm, and athlete’s foot. • Mold allergies are very common.

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Use this resource to answer the questions that follow.

55 1.16. Fungi and Human Disease www.ck12.org

FIGURE 1.26 Ringworm produces a ring-shaped rash, but it isn’t caused by a worm. It’s caused by the same fungus that causes athlete’s foot.

FIGURE 1.27 The mold growing on the walls and ceil- ing of this storm-damaged home may be harmful to human health.

• Types of Fungal Diseases at http://www.cdc.gov/fungal/diseases/index.html.

1. Describe the following fungal diseases: a. Pneumocystis pneumonia b. Candidiasis c. Fungal Keratitis

56 www.ck12.org Chapter 1. Protists and Fungi

d. Histoplasmosis

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Review

1. Explain why you should never eat mushrooms you ﬁnd in the woods unless you know for certain which type of mushrooms they are. 2. Compare and contrast ringworm and athlete’s foot. 3. How does mold cause allergies? 4. State why indoor mold may cause more health problems than outdoor mold.

Review Answers

1. If people eat toxic fungi, they may experience digestive problems, hallucinations, organ failure, and even death. Most cases of mushroom poisoning are due to mistaken identity. That’s because many toxic mushrooms look very similar to safe, edible mushrooms. So, you should never eat mushrooms you find in the woods unless you know for certain which type of mushrooms they are. 2. Ringworm is a fungal skin infection characterized by a ring-shaped rash. The rash may occur on the arms, legs, head, neck, or trunk. The same fungi cause athlete’s foot when they infect the skin between the toes. 3. Mold allergies are caused by airborne mold spores. When the spores enter the respiratory tract, the immune system responds to them as though they were harmful microbes. Symptoms may include sneezing, coughing, and difficulty breathing. 4. Indoor mold may cause more health problems than outdoor mold because of the closed, confined space. Most people also spend more time indoors than out.

Summary

Protists and Fungi discusses these two types of eukaryotic organisms. What do they have in common? Protists are the earliest eukaryotes, and this kingdom contains some of the simplest eukaryotes. Many are single-celled organisms. Protists consist of animal-like, plant-like, and fungus-like species. Protists evolved into the other three types of eukaryotes, including fungi. Other than that, these two types of eukaryotes are very different. Fungi are eukaryotic organisms that cannot make their own food and do not "eat." They must absorb their nutrients, usually from decaying organisms.

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1.17 References

1. Laura Guerin. Diversity of protists. CC BY-NC 3.0 2. . . CC-BY-NC-SA 3.0 3. Miranda Dudzik. Forms of protist motility. CC BY 3.0 4. User:Spicywalnut/Wikipedia. Spirogyra has a complex life style. Public Domain 5. Diatoms: Courtesy of Prof. Gordon T. Taylor, NOAA; Seaweed: Wilfredo R Rodriguez H. Both unicellular diatoms and multicellular seaweed are algae. Public Domain 6. Claire Fackler, NOAA. Kelp forests help sustain large communities. CC BY 2.0 7. Mariana Ruiz Villarreal (LadyofHats) for CK-12 Foundation. Life cycles of algae. CC BY-NC 3.0 8. Jason Hollinger. “Dog vomit” slime mold. CC BY 2.0 9. Courtesy of the Centers for Disease Control and Prevention. . Public Domain 10. User:Petaholmes/Wikimedia Commons. Worldwide distribution of malaria. Public Domain 11. User:Ciar/Wikimedia Commons. Fungal mold growing on bread. Public Domain 12. Mushrooms: Jonathan Dinh; Yellow Staghorn: milo bostock (milesmilob); Jelly Ears: jenny downing; Mold: User:Sapp/Wikimedia Commons; Blue Turkeytail: Flickr:Aah-Yeah. Examples of fungi: mushroom, yellow staghorn, jelly ears, mold, blue turkeytail. Mushrooms: CC BY 2.0; Yellow Staghorn: CC BY 2.0; Jelly Ears: CC BY 2.0; Mold: Public Domain; Blue Turkeytail: CC BY 2.0 13. Image copyright Andre Nantel, 2014. Hyphae of the penicillium mold. Used under license from Shutter- stock.com 14. H. Krisp. Giant "humongous fungus" can cover vast areas. CC BY 3.0 15. A Bremner. Forest decomposers perform an important job. CC BY 2.0 16. User:Lex vB/Wikipedia. . Public Domain 17. Image copyright siloto, 2014. Puffballs release spores when disturbed.. Used under license from Shutter- stock.com 18. Courtesy of National Institute of Allergy and Infectious Diseases/National Institutes of Health. Yeast reproduce asexually by budding. Public Domain 19. User:Jpwilson/Wikipedia. This rock contains fossilized fungi. Public Domain 20. Flickr:SNappa2006. Moss(plant) and lichen growing next to each other on a tree. CC BY 2.0 21. Image copyright Dr. Morley Read, 2014. Parasitic fungus and insect host. Used under license from Shutter- stock.com 22. User:Hardyplants/Wikipedia. Lichen growing on rock. Public Domain 23. Derek Wolfgram. Edible types of mushrooms. CC BY 2.0 24. U.S. Department of Agriculture. Blue cheese has mold growing in it.. CC BY 2.0 25. Destroying Angel: DeviantArt:Celes247; Edible Puffball: Martin Pettitt. Toxic destroying angel mushroom compared to edible puffball mushroom. Destroying Angel: CC BY 3.0; Edible Puffball: CC BY 2.0 26. Ringworm: Dr. Lucille K. Georg/Centers for Disease Control and Prevention; Athlete’s foot: User:Falloonb/Wikipedia. Ringworm and athlete’s foot are fungal diseases. Public Domain 27. User:Infrogmation/Wikimedia Commons. Indoor mold can be harmful to human health. CC BY 3.0