Common Laboratory Equipment

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COMMON LABORATORY EQUIPMENT Erlenmeyer Flask Beaker May be used to Probably the most hold liquids, instead common vessel for of beakers, when a holding liquids in smaller opening is the laboratory. Not preferred. Not used used for accurate for accurate measurements. measurements. Test Tube Graduated Used for many Cylinder activities, which Used to measure require multiple the volume of reagents or liquids. solutions. Test Tube Rack Test Tube Holder Holds many test A test tube holder is tubes so that they useful for holding a do not break and test tube that is too their contents do hot to handle. not spill. Stoppers Funnel Rubber and cork Funnels are used to stoppers are used aid in the transfer of to close test tubes liquids, powders or and flasks, thus solids from one avoiding spillage or vessel to another. contamination. Glass Stirring Rod Scoopula A glass rod is used Used to transfer to stir solutions or chemicals solids to transfer a single from one vessel to drop of a solution. another. Dropper Pipette Beaker Tongs Used to transfer Used to handle hot small amounts of beakers and other liquid. glassware Forceps Goggles Forceps are used Must be worn for to hold or pick up each lab activity. small objects. COMMON LABORATORY EQUIPMENT Metric Ruler Thermometer We always use the A thermometer is Metric System to used to measure make our temperatures. measurements. Ring Stand Iron Ring Used in many lab Often attached to activities as the ring stand to use support for other as support for a apparatus. beaker. Clamp Clay Triangle May be attached to Can be placed on a ring stand and be an iron ring to made to hold a test provide a stage for tube or a crucible. thermometer. Crucible Wire Gauze Crucibles are used Often placed over for heating certain the iron ring, to solids, particularly provide a "stage" metals, to very high for a beaker. temperatures. Bunsen Burner Sparker Used to heat Used to ignite the materials in lab. Bunsen burner. Wash Bottle Watch Glass A wash bottle has Used to evaporate a spout that off the liquid part of delivers a wash a solution or hold a solution to a solid for a reaction. specific area. Electronic Balance Used to find the Hot Plate mass of materials. Used for heating This particular liquids. balance is not as precise as the beam balance .

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Lab Equipment Erlenmeyer Flask
Lab Equipment Erlenmeyer Flask • Erlenmeyer flasks hold solids or liquids that may release gases during a reaction or that are likely to splatter if stirred or heated. Beaker • Beakers hold solids or liquids that will not release gases when reacted or are unlikely to splatter if stirred or heated. Graduated Cylinder • A graduated cylinder is used to measure volumes of liquids. Test Tubes • Test tubes are used to hold small amounts of liquid and to conduct small chemical reactions in. Test Tube Clamp • A test tube holder is useful for holding a test tube which is too hot to handle. Test tube and beaker Brushes • Test tube brushes are used to clean test tubes and graduated cylinders. • Forcing a large brush into a small test tube will often break the tube. Test Tube Racks • Test tube racks are for holding and organizing test tubes on the laboratory counter. Plastic racks may melt in contact with very hot test tubes. Spot Plates • Spot plates are used when we want to perform many small scale reactions at one time. We will use these many times during the year. Watch Glass • A watch glass is used to hold a small amount of solid, such as the product of a reaction. Glass Stir Rod • A glass rod is used to manually stir solutions. It can also be used to transfer a single drop of a solution. Funnel • A funnel is used to aid in the transfer of liquid from one vessel to another. • A funnel is used to separate a solid from a liquid Wash Bottle • A wash bottle has a spout that delivers a wash solution to a specific area.
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Microinjection & Transfection 2016-2017
WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas Microinjection & Transfection 2016-2017 Come see us at The Allied Genetics 2016 Conference • July 13-17 • Booths # 623 & 816/818 Everything you need for Microinjection Microinjection processes use either metal microinjection Recently, WPI introduced its customizable Microinjection needles or glass micropipettes to inject small liquid System with everything you need to get started. We can volumes. For example, genetic material may be inserted help you customize your system with many options and into a living cell, a drug introduced into an eye or brain, microinjection system accessories. The basic system or fluid injected into a muscle. Typically, microinjection includes: is performed under a microscope. A stereotaxic frame l Microinjection pump like the PV820 Pneumatic setup may be required. PicoPump l LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror l Micromanipulator l Many accessories WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures. Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application. Options for Customizing Your System INJECTOR ✱ PV820 Pneumatic PicoPump with Hold Pressure • PV830 Pneumatic PicoPump with Hold Designed to simplify intracellular injection and a variety Microprocessor- The versatile Pressure and Vacuum of other micro in jec tion tasks, WPI's PicoPumps use UMP3 controlled injector uses • Nanoliter2010 carefully regulated air pres sures for se cur ing cells and injecting them with fluid.
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Determination of the Identity of an Unknown Liquid Group # My Name the Date My Period Partner #1 Name Partner #2 Name
Determination of the Identity of an unknown liquid Group # My Name The date My period Partner #1 name Partner #2 name Purpose: The purpose of this lab is to determine the identity of an unknown liquid by measuring its density, melting point, boiling point, and solubility in both water and alcohol, and then comparing the results to the values for known substances. Procedure: 1) Density determination Obtain a 10mL sample of the unknown liquid using a graduated cylinder Determine the mass of the 10mL sample Save the sample for further use 2) Melting point determination Set up an ice bath using a 600mL beaker Obtain a ~5mL sample of the unknown liquid in a clean dry test tube Place a thermometer in the test tube with the sample Place the test tube in the ice water bath Watch for signs of crystallization, noting the temperature of the sample when it occurs Save the sample for further use 3) Boiling point determination Set up a hot water bath using a 250mL beaker Begin heating the water in the beaker Obtain a ~10mL sample of the unknown in a clean, dry test tube Add a boiling stone to the test tube with the unknown Open the computer interface software, using a graph and digit display Place the temperature sensor in the test tube so it is in the unknown liquid Record the temperature of the sample in the test tube using the computer interface Watch for signs of boiling, noting the temperature of the unknown Dispose of the sample in the assigned waste container 4) Solubility determination Obtain two small (~1mL) samples of the unknown in two small test tubes Add an equal amount of deionized into one of the samples Add an equal amount of ethanol into the other Mix both samples thoroughly Compare the samples for solubility Dispose of the samples in the assigned waste container Observations: The unknown is a clear, colorless liquid.
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Funnels and Filters
51 FUNNELS AND FILTERS Buchner Funnels 260MM (10.25") INSIDE DIAMETER, 203MM (8") OVERALL HEIGHT, 127MM (5") RIM TO PLATE CATALOG NO. DESCRIPTION PRICE/EACH H14620-0000 Funnel with Coarse Porosity Fixed Plate $216.00 H14620-1260 Funnel with Coarse Porosity Removable Plate 216.00 H14625-3510 Funnel with Medium Porosity Fixed Plate 216.00 H14626-3510 Funnel with Medium Porosity Removable Plate 216.00 H14627-0000 Funnel with Perforated Fixed Plate 216.00 Table-Top H14627-1260 Funnel with Perforated Removable Plate 216.00 Buchner 457MM (18") INSIDE DIAMETER, 292MM (11.5") OVERALL HEIGHT, 203MM (8") RIM TO PLATE Funnels CATALOG NO. DESCRIPTION PRICE/EACH With Fritware® Porous H14621-0000 Funnel with Coarse Porosity Fixed Plate $540.00 Filter or HDPE H14621-1457 Funnel with Coarse Porosity Removable Plate 540.00 H14625-3518 Funnel with Medium Porosity Fixed Plate 540.00 Perforated Plate H14626-3518 Funnel with Medium Porosity Removable Plate 540.00 Polyethylene funnels are H14628-0000 Funnel with Perforated Fixed Plate 465.00 one piece design with a H14627-1457 Funnel with Perforated Removable Plate 465.00 FIXED or REMOVABLE Custom sizes available upon request. 610MM (24") INSIDE DIAMETER, 330MM (13") OVERALL HEIGHT, 267MM (10.25") RIM TO PLATE porous filter plate CATALOG NO. DESCRIPTION PRICE/EACH supported by a multiple ring grid. Filter plates are made of high density H14622-0000 Funnel with Coarse Porosity Fixed Plate $1125.00 1 polyethylene 6.4mm ( ⁄4") thick with a non-porous ring at the periphery of the plate H14622-1610 Funnel with Coarse Porosity Removable Plate 1125.00 1 H14625-3524 Funnel with Medium Porosity Fixed Plate 1125.00 which seals filter paper when used.
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Maintenance of Bacterial Strains
Maintenance of Bacterial Strains Short-term growth and maintenance The simplest method for obtaining strains is to order them from a microbiological supply company. Presque Isle, Ward, and Carolina Biological (see “Resources” page of the Microbes Web site: www.umsl.edu/~microbes) are economical sources of strains that are commonly used in the classroom. Another possible source is a local college or university that teaches a microbiology lab course. If they have strains already prepared for their class, they will probably be willing to provide you with a culture. This is probably not a reasonable ongoing source of strains, but can be useful in an emergency. For short-term maintenance and use, it is best to streak bacteria on some type of rich agar petri plate, such as nutrient agar. Label and date the plate on the bottom of the plate. Streak from the source culture using good sterile technique. The quadrant streak method (see the article on quadrant streaking) is good for obtaining well-isolated colonies. Incubate the plate only until good colonies have formed. Do not leave the culture in the incubator beyond that time, or the cells will die on the plate. Store the plate in the refrigerator. It will keep for about a month. For class use, it is best to streak a fresh plate 2-3 days before the strain will be needed for class. Use the fresh plate as the source of cultures for the class. For broth cultures for a class, it is best to grow a larger volume culture in a flask (if possible, with shaking) overnight and then dispense aliquots of the master culture into sterile test tubes using a sterile pipette.
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EXPERIMENT 15 TF Notes
EXPERIMENT 15 TF Notes 1. Have students log onto LoggerPro3 and start heating water as soon as they arrive 2. This reaction is easily contaminated so students must never insert the thermometer directly into the cuvette containing the reaction. Instead they should rather measure the temperature of the water and equate that to the temperature of the solution in the cuvette. 3. We will be using hot gloves in this experiment. 4. Make sure you are familiar with the calculations necessary to fill out the chart. All the necessary equations are given at the top fo the page. Note that the temperature must be converted from °C to K. EXPERIMENT 15 Thermodynamics of Complex-Ion Equilibria Introduction Thermodynamic data for a reaction system provides researchers with information that is important from both theoretical and practical points of view. There are several thermodynamic properties that chemists pay close attention to when designing or carrying out experiments such as thermodynamic stability, the change in free energy of a reaction, and temperature dependence. For example, if a chemist wants to create a new type of solar cell that combines a semiconductor material with a novel conductive oxide and wants to make sure that the two materials will not react with each other, thermodynamics provide the answer. By finding the free energy change associated with the reaction, s/he can determine how stable the layers are in contact with each other and to what temperature. In this experiment, you will learn how to determine those parameters from a controlled experiment by using spectrometry to find concentration data at various temperatures.
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Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position.
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Manual: Zetasizer Nano Accessories Guide
! www.malvern.com Zetasizer nano series Malvern Instruments Limited Printed in England MRK1378-01 Enigma Business Park Q & Grovewood Road, Malvern Worcs, WR14 1XZ, U.K. SelAccessories Guide Tel: +44 (0) 1684 892456 Fax: +44 (0) 1684 892789 Malvern Zetasizer Nano accessories guide MAN0487 Issue 1.1 April 2013 Copyright © 2007 - 2013 Malvern Instruments Ltd. Malvern Instruments pursues a policy of continual improvement due to technical development. We therefore reserve the right to deviate from information, descriptions, and specifications in this publication without notice. Malvern Instruments shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material. No reproduction or transmission of any part of this publication is allowed without the express written permission of Malvern Instruments Ltd. Head office: Malvern Instruments Ltd. Enigma Business Park, Grovewood Road, Malvern, Worcestershire WR14 1XZ United Kingdom. Tel + [44] (0)1684-892456 Fax + [44] (0)1684-892789 Zetasizer, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries, and are owned by Malvern Instruments Ltd. NIBS and M3-PALS are trademarks of Malvern Instruments. M3 is granted Euro Pat No: 1 154 266 DE FR. Windows is a registered trademark of Microsoft Corporation. Tygon is a registered trademark of Saint-Gobain Corporation. Hellmanex is a registered trademark of Hellma GmbH & Co. KG. Table of contents Introduction and accessory range Introduction . 1-1 Accessory range. 1-1 General cells and cuvettes Introduction . 2-1 Cuvette holder . 2-2 Cell and cuvettes . 2-3 Size and molecular weight cuvettes .
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Portable Fluid Analysis Kit Manual
Donaldson Delivers Portable Fluid Analysis Kit Patch Test Kit Manual Kit Part Number X009329 Carrying Case Membrane Holder & Funnel Assembly P567863 Filter for Solvent Dispensing Bottle P567860 (Qty. 3) 500 ml Solvent Sampling Dispensing Bottle Pump P176431 Microscope 120 ml P567864 Sample Bottles (Qty. 6) Plastic Tubing P567861 (5 ft.) 1.2 micron Patch Covers Membrane P567912 (Qty. 150) Filters P567869 Zip Drive of (Qty. 100) Reference Information Membrane Filter Forceps Sharpie Analysis Cards (3”x5”) P567865 (Qty. 50) 5 micron Membrane Filters Marker P567868 (Qty. 50) Case Size: Height: 14.5”/368.3mm | Width: 19.25”/489mm | Depth: 7.75”/197mm | Case Weight: 9.95 lbs./4.51 kg 1 2 3 Assemble waste bottle, funnel- Install solvent* dispensing tube and Rinse the funnel-patch assembly patch assembly, and vacuum pump install solvent filter on end of the with the filtered solvent to remove to form the sample processing dispensing tube. background contamination. The assembly. Tighten the vacuum patch should not be in place for pump o-ring on the funnel-patch * Mineral spirits are the most this process. assembly tube by turning the commonly used solvent aluminum locking device. 2 • www.donaldson.com Patch Test Kit Manual 4 5 6 Separate the funnel from the patch Reattach the funnel to the filter Agitate the sample fluid bottle and supporter and install a filter patch patch base with filter patch. Twist pour 25ml into the funnel. 25ml with ink grid up. (If the patch has an lock the funnel to the base. is denoted by the first line on the ink grid) funnel (closest to the patch).
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Marsh Funnel
Marsh Funnel Summary: The Marsh Funnel was developed to provide a field measurement of the apparent viscosity of clay-thickened drilling mud. Provided that a conversion table has been developed, a modified Marsh Funnel can be used to provide a measure of apparent viscosities for gum-thickened and polymer- thickened products used in wildland fire operations. Without a conversion table, the flow-through times can be used to determine batch to batch consistency of a single product, and in limited circumstances to compare similar products. The directions for use of a Marsh Funnel are given below. The procedures for modifying the Marsh Funnel for use in determining the viscosity of long-term retardants (Forest Service modification) and the consistency of water enhancers (California Department of Forestry and Fire Protection, CDF modification) are provided in the following pages. Materials: Marsh Funnel (Use small tip, 3/16” diameter, for low-viscosity products such as Phos-Chek 259 or Phos-Chek LC-95A. Use the large tip, 17/64” diameter, for high- viscosity products such as Phos-Chek D75.) Container marked to 1 quart, a tall straight-sided container is best Stopwatch or timer with a “time up” function Thermometer Method: 1. Allow the test sample to stand until it reaches room temperature (70°F) and all air bubbles are dissipated since both of these factors have an influence on viscosity. 2. Ensure that the properly modified funnel is clean and dry and secured in an upright position (a ring stand works fine). 3. Cover the funnel orifice with a finger while pouring the test sample through the screen until the sample exactly reaches the bottom of the screen.
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Chapter 2 2.1 Description of Kit Items
Manual of SEAT Friendly MCL Kit 7 Chapter 2 2.1 Description of Kit Items S.No. Item Name Figure/Setup Uses (Quantity/ kit) 1. Storage box opens on both sides. Contains necessary 1. Wooden box apparatus for doing with revolving experiments. top 2. Revolving top containing (1) dispensing bottles and vials for easy access to chemicals. 1. As a container for keeping liquids. 2. Microbeaker (10 mL) (12) 1. As a container for use in electrochemical experiments, 3. Beaker (50 thermochemistry experiments mL) preparation of salts, etc. (3) Steps for use 1. Bore the rubber cork to fit the stem of Hirsch funnel. 2. Cut the filter paper to fit the mesh of the funnel. The size should exactly fit the mesh, 4. Micro- neither big nor small. filtration 3. Fit the cork with funnel in unit the mouth of the boiling tube (2) (having a side tube). 4. Transfer the solution to be filtrated to the funnel. 5. Press the bulb with hand to evacuate it and place it on the side tube of the boiling tube while keeping it pressed. 6. Release the pressure. The bulb will suck the air inside the boiling tube allowing fast filtration. 8 Manual of SEAT Friendly MCL Kit S.No. Item Name Figure/Setup Uses (Quantity/Kit) 1. The watch glass is used to hold solids when being 5. Watch glass weighed. (2) 2. It should never be heated. 1. Flat plate with multiple “wells” used as micro test tubes. 2. Contains 96 rectangular matrix. 3. Each well of a micro plate typically holds somewhere between a few to a few hundred microlitres of liquid.
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