EVOLUTIONARY HISTORY of Cedrela (MELIACEAE) in CENTRAL BRAZIL
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ALEXANDER HUAMÁN-MERA EVOLUTIONARY HISTORY OF Cedrela (MELIACEAE) IN CENTRAL BRAZIL Thesis submitted to the Botany Graduate Program of the Universidade Federal de Viçosa, as part of the requirements to obtain the title of Doctor Scientiae. VIÇOSA MINAS GERAIS – BRAZIL 2018 i To my parents, Mardóneo and Yolanda and my family in my country, Peru, to my own family, Yuriko and Akira, And to Scientia amabilis ii ACKNOWLEDGMENTS To my wife Yuriko and my little son Alexander Akira, for their support, patient and constantly love. To the Programa de Pós-graduação em Botânica and its professors of the Universidade Federal de Viçosa (UFV) for the new aknowlegments I acquired during my stage as doctoral student. To Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the grants and scholarship fundings. To my Professor Luiz Orlando de Oliviera for the guidance, dedication and especially by stimulated my interest in the Evolutionary Biology. To Juan Manuel Díaz-Soto who unceasingly supported in fieldwork and lab work of the present study, as well as for his friendship and scientific support. To my friends and colleagues in the Laboratory of Biologia Molecular e Filogeografia: Jefferson, Rafaela, Thaís, Thamyres, and Tiago; and to my friends who are no more in the laboratory: Érica, Hugo, Khalid, Leandro, and Thiago, for their companionship and friendship. To my professors of the Departamento Académico de Botánica of the Universidad Nacional Pedro Ruiz Gallo: Guillermo Delgado Paredes, Consuelo Rojas Idrogo, Leopoldo Vásquez Nuñes, and Josefa Escurra Puicón for their support and teachings during my undergraduate course. iii BIOGRAPHY ALEXANDER HUAMÁN-MERA, son of Mardóneo Huamán Chávez and Yolanda Mera Cayao, was born in Chomza Alta, La Peca, Bagua, Amazonas – Peru, on July 25th 1983. He completed his elementary education in 1996 and high school in 1999 at the "Juan Pablo Guzmán y Vizcardo Zea" high school in La Victoria, Chiclayo, Peru. He concluded his undergraduate course in the Facultad de Ciencias Biológicas of the Universidad Nacional "Pedro Ruiz Gallo", Lambayeque – Peru, in October 2006. He also completed a specialization in Botany during his undergraduate studies. In July 2011 he obtained the Professional title of Biologist–Botanist. In February 2014, he obtained the degree of Masgister Scientiae in Botany at the Botany Graduate Programa of the Universidade Federal de Viçosa, Minas Gerais – Brazil. His major was in the area of Systematic of Phanerogams. In March 2014, he began as Doctoral student in the Botany Graduate Programa of the Universidade Federal de Viçosa, Minas Gerais, Brazil. iv SUMARY ABSTRACT ........................................................................................................................................... vii RESUMO .............................................................................................................................................. viii I. GENERAL INTRODUCTION ........................................................................................................ 1 1.1 The central Brazil ..................................................................................................................... 1 1.2 The Neotropical genus Cedrela (Meliaceae) ............................................................................ 1 1.3 The hotspot Cerrado, Neotropical Forests, and tropical refugies ............................................. 2 1.4 Brazilian seasonal Forest .......................................................................................................... 4 1.5 Niche phylogenetic conservatism of Cedrela and Species Distribution Modeling (SDM)...... 4 II. REFERENCES ................................................................................................................................. 6 CHAPTER I: CLIMATIC FLUCTUATIONS OF TERTIARY/QUATERNARY BOUNDARY AS THE DRIVERS OF NEW LINEAGES OF Cedrela (MELIACEAE) IN THE CENTRAL BRAZIL .... 9 ABSTRACT ........................................................................................................................................... 10 I. INTRODUCTION .......................................................................................................................... 11 II. MATERIALS AND METHODS ................................................................................................... 14 2.1 Sampling Strategy and DNA extraction ................................................................................. 14 2.2 Microsatellite analyses ........................................................................................................... 18 2.3 cpDNA and ITS datasets Sequencing .................................................................................... 20 2.4 Supplementary information from GenBank ........................................................................... 23 2.5 Bayesian phylogenetic analyses ............................................................................................. 25 2.6 Divergence dating .................................................................................................................. 25 2.7 Species Distribution Modeling (SDM)................................................................................... 26 III. RESULTS ....................................................................................................................................... 27 3.1 Analyses of microsatellite loci ............................................................................................... 27 3.2 Bayesian phylogenetic analyses ............................................................................................. 31 3.3 Estimated date of divergence ................................................................................................. 36 3.4 Species Distribution Modeling (SDM)................................................................................... 38 IV. DISCUSSION ................................................................................................................................ 45 4.1 New lineages restricted to central Brazil ................................................................................ 45 4.2 Different historical origins of lineages of Cedrela ................................................................. 45 4.3 Recent wave of colonization of Cedrela brachystachya in central Brazilian plateau ............ 46 4.4 Convergence of hybridization zones of Cedrela fissilis and others Cedrela lineages ranges.47 4.5 Displacement of niche of the Lineage from Northern Minas Gerais ..................................... 48 4.6 Conservation implications of current distribution of Cedrela lineages .................................. 48 4.7 Morphological correspondence of new Cedrela lineages ...................................................... 49 v V. CONCLUSION .............................................................................................................................. 50 VI. REFERENCES ............................................................................................................................... 55 CHAPTER II: TWO NEW SPECIES AND NEW OCCURRENCES OF Cedrela (MELIACEAE) FROM CENTRAL BRAZIL .................................................................................................................. 61 ABSTRACT ........................................................................................................................................... 62 I. INTRODUCTION .......................................................................................................................... 63 II. MATERIALS AND METHODS ................................................................................................... 65 2.1 Sampling ................................................................................................................................ 65 2.2 Morphometric analyses .......................................................................................................... 68 III. RESULTS ....................................................................................................................................... 68 3.1 Taxonomic treatment.............................................................................................................. 68 3.1.1 Cedrela microanthus Huamán-Mera A. and Oliveira LO, sp. nov.. ................................... 68 3.1.2 Cedrela pandeirensis Huamán-Mera A. and Oliveira LO, sp. nov..................................... 73 3.2 New occurrences of Cedrela brachystachya in central Brazil ............................................... 78 IV. DISCUSSION ................................................................................................................................ 79 4.1 Morphometric analyses of Cedrela microanthus ................................................................... 79 4.2 Morphometric analyses of Cedrela pandeirensis ................................................................... 80 4.3 Conservation status of new taxa of Cedrela ........................................................................... 83 V. REFERENCES ............................................................................................................................... 84 vi ABSTRACT HUAMÁN-MERA, Alexander, D.Sc., Universidade Federal de Viçosa, June, 2018.