Australian Society for Parasitology Inc. ABN 65 979 686 445

PO Box 60 Royal Brisbane Hospital QLD, Australia, 4029

Web: www.parasite.org.au Email: [email protected] Tel: +61 7 42321311 Introduction

I AM DELIGHTED TO PRESENT TO YOU THE 2016 ANNUAL REPORT FOR THE AUSTRALIAN SOCIETY FOR PARASITOLOGY INC., WHICH HAS BEEN PREPARED BY OUR ASP NETWORK TEAM, LISA JONES, IAN HARRIS AND NICK SMITH.

Research Achievements

Parasitology research in Australia continued to flourish, with over 550 research papers published in 2016 – this is a record and continues the increase in quantity and quality of research outputs produced by ASP members that has been seen for the last decade. At the same time, our journals have attained new records in citation statistics. I thank very much our journal leadership teams: Maria Meuleman, Brian Cooke, Alex Loukas and Jan Slapeta (The ASP President, David Emery International Journal for Parasitology); Kevin Saliba and Andrew Koetze (IJP: Drugs and Drug Resistance); Andrew Thompson and Researcher Exchange, Training and Travel Awards Lydden Polley (IJP: Parasites and Wildlife); as well as Dale Seaton, from Elsevier, the publisher of all three of our journals, for his The ASP continued to offer Researcher Exchange, Training and unwavering support. Travel Awards and its Mentorship Scheme in 2016, continuing to look to collaborations with international colleagues to help In 2016, funding for parasitology research bounced back from us maintain a leading global reputation in parasitology. Details a sustained 3 year low point, with over 40 grants or fellowships and highlights of these awards appear elsewhere in this annual worth over $33 million awarded to ASP members; this is very good report. These small grants have the ability to change careers of news for our discipline. It is noteworthy that much of this success our young researchers and it is pleasing to see that, in 2016, past occurred outside of the ARC and NHMRC schemes, including recipients again won fellowships or grants to fund their research. significant international funding. I thank sincerely the Researcher Exchange, Training and Travel Assessment Committee, Una Ryan, Geoff McFadden, Kate Hutson, Full lists of Australian parasitology publications and research Nick Smith and Lisa Jones, for all their hard, impartial and diligent grants are included as Appendix 1 and Appendix 2 to this annual work. report.

Page 1 Introduction

Honours for their unique integration of mathematicians, computer scientists and physicists, in close collaboration with experimental The ASP honoured several extremely deserving parasitologists scientists and clinicians, to develop a novel understanding of in 2016: Denise Doolan was awarded the Bancroft-Mackerras the ‘mathematics of infection’ using these insights to design Medal; Alexander Kennedy and Hanh Nguyen won JD Smyth and optimise treatment and vaccination for major infectious Awards; Russell Stothard, Peter Crompton and Maria Dolores diseases (finalists in the UNSW Eureka Prize for Excellence in Bargues. were named ASP International Lecturers; Dhanasekaran Interdisciplinary Scientific Research category). Peter Holdsworth Sakhivel and Mohammed Al-Hasnawy, were runner-up and winner, received an AM “for distinguished service to veterinary respectively, of Best Student Poster; Erick Tjhin and Deepani science, particularly to animal parasitology and pharmaceutical Fernando, were runner-up and winner, respectively, of Best development, and to professional scientific organisations”. Freya Student Presentation; and Sam Emery and Adele Lehane, runner- Fowkes of the Burnet Institute won the prestigious Georgina Sweet up and winner, respectively, of Best Early Career Researcher Award for Women in Quantitative Biomedical Science. Alex Maier Presentation at the annual conference. Other organisations also was awarded the ANU’s Vice-Chancellor's Award for Public Policy honoured several of our members. Continuing a fine winning and Outreach, while Vicky Avery and Melissa Sykes won Griffith tradition for parasitology at the prestigious Eureka Prizes, Leann University’s Pro Vice Chancellor’s Research Excellence Awards for Tilley, Nick Klonis, Julie Simpson and James McCaw, from The Mid-Career and Early Career Researcher, respectively. University of Melbourne, took out the Australian Infectious Diseases Research Centre Eureka Prize for Infectious Diseases Research for their key scientific discoveries leading to insights Annual Conference into how artemisinin resistance by the malaria parasite may be overcome. Congratulations also to several other finalists for this The 2016 ASP Conference was held in conjunction with the year’s awards including: The Boddey, Sleebs and Cowman team, Internationall Congress for Tropical Medicine and Malaria, in the Walter and Eliza Hall Institute of Medical Research, in the partnership with the Australasian Society for Infectious Disease, Australian Infectious Diseases Research Centre Eureka Prize for in Brisbane in September, 2016. The meeting was a resounding Infectious Diseases Research category for their research into success, attracting over 1500 delegates and returning a healthy determining how malaria parasites use a unique protein export profit to the Society. The ASP is extremely grateful to the pathway across the parasite’s lifecycle, enabling collaboration conference organising and scientific committees (David Looke, with industry that is accelerating the development of a new Denise Doolan, Kathy Andrews, James McCarthy, Paul Griffin, Tina antimalarial drug; The SHIFT Team, a collaboration between Skinner-Adams, Lisa Jones, Marilena Salvo, Roland Cobbold, Chris Murdoch Childrens Research Institute, The Kirby Institute, St Coulter, Bart Currie, Gregor Devine, Katja Fischer, Katie Flanagan, Vincent’s Hospital, Menzies School of Health Research and the Fiji Robin Gasser, Paul Johnson, Cheryl Jones, Colleen Lau, Alex Ministry of Health that has produced a landmark study showing Loukas, Deborah Marriott, Weiland Meyer, Clare Nourse, Geoffrey that mass drug administration with the oral drug ivermectin Playford, Jenny Robson, Harsha Sheorey, Nick Smith, Wai-Hong is highly effective in controlling scabies and related bacterial Tham, Joseph Torresi, Nicole Townell, Mark Walker, Trent Yarwood) skin sores, transforming the global conversation on integrated but, most especially, Mal Jones for his tireless enthusiasm in programs for neglected tropical diseases (finalist in the Australian putting the conference together. The Society also owes a great Infectious Diseases Research Centre Eureka Prize for Infectious debt to its army of conference volunteers, Thomas Williams, Diseases Research category); and, Miles Davenport, Deborah Leanne Low, Gillian Fisher, Thomas Teoh, Christopher Hart, Cara Cromer, Mykola Pinkevych and David Khoury, Kirby Institute, Wilson, Ellie Stellar, Jessie van Huis, MJ Chua, Naomi Clarke, Bilal UNSW with Stephen Kent, Peter Doherty Institute, The University Zulfiqar, David Teran, Megan Arnold, Jess Bridgford, Amy Jones of Melbourne, and Ashraful Haque, QIMR Berghofer Institute, and Maria Meuleman. The conference was sponsored generously

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by the Bill and Melinda Gates Foundation, the International Journal (Tasmanian rep.), Benedikt Ley (NT rep.), Abdul Jabbar and Nathan for Parasitology, Elsevier, the Georgina Sweet Travel Support Bott (Victorian reps), Mark Pearson and Gillian Fisher (QLD reps), Fund, the Boehringer Ingelheim Foundation, the QIMR Berghofer Stephanie Godfrey and Crystal Cooper (WA reps), Tina Skinner- Institute of Medical Research, Compounds Australia, Griffith Adams (Incorporation Secretary), Mal Jones (Bancroft-Mackerras University, The University of Queensland, Cambridge University Medal Convenor), Brian Cooke (IJP Editor), Kevin Saliba and Press, IVCC, the Walter and Eliza Hall Institute of Medical Andrew Kotze (IJP:DDR Editors), Andy Thompson (IJP:PAW Editor), Research, New England Biolabs, the Australian Infectious Disease Haylee Weaver (Archivist), Nick Smith (Network Convenor) Research Centre and the Doherty Institute. More details about our and Lisa Jones (Newsletter Editor, Webmaster and Network conference can be found inside this annual report. Communications Coordinator).

I must finally state that I am indebted to the dogged and dedicated Public Engagement and Outreach efforts of the Treasurer, Peter Rolfe, who has single-handedly revised and restructured the Society’s financial reporting and Lisa Jones, yet again, was awarded a a Commonwealth of Australia business systems over the past 2 years. This has been a Herculean National Science Week grant through the Department of Industry task but a momentous outcome for the smooth functioning of the and Science for two events at The Tanks Arts Centre in Cairns: society. Thank you Peter. Café Scientifique: Science, Music & Art – All in the Mind on Tuesday 16th August; and Pecha Kucha Night Cairns inspired by science on David Emery Wednesday 17th August. Lisa continues to be an inspiration for President of the ASP us all as evidenced by ASP members’ remarkable willingness and enthusiasm to embrace community engagement as a core activity of the Society throughout 2016 (see the highlights inside this annual report).

Advanced Parasitology Course

The year closed with our third Advanced Parasitology Course – Concepts in Parasitology, held at ANU’s field station at Kioloa, on the NSW south coast; it is a continuing source of great pride for the Society and I thank Alex Maier for again coordinating a brilliant course. More information about the course within…

Our Council

Finally, I must thank the members of the Council of the Society, all of whom work enthusiastically on behalf of all members. My thanks to Peter Rolfe (Treasurer), Colin Stack (Executive Secretary), Cover: A young visitor to the exhibition Parasites: Life Undercover at Una Ryan (President-Elect), Giel van Dooren (ACT rep.), Danny the Queensland Museum, August 2016. Parasites: Life Undercover was supported by the Australian Society of Parasitology and ran from 10 August Wilson (SA rep.), Shokoofeh Shamsi (NSW rep.), Barbara Nowak 2016 – 27 January 2017. The exhibition is featured later in the report.

Page 3 Images from ICTMM/ASP 2016

Page 4 Contents

Introduction by David Emery, President of the ASP...... 1 Research Achievements ...... 6

Features: New hope for malaria (8), Open source drug discovery (10), Lightowlers in Nepal (12)

Researcher Exchange, Training and Travel Awards ...... 13

Features: Dhanasekaran Sakthivel, (15), Alejandro Trujillo Gonzalez (17), Ali Raza (18), Hanh Nguyen (20) Alexander Kennedy (21), Kate Hutson (23),

Honours...... 25

Features: Freya Fowkes (26), Denise Doolan, BMM Medal 2016 (27)

ICTMM/ASP Annual Conference...... 30

Features: Opening ceremony (31), ECR Breakfast (32), Annual General Meeting (33) An interview with Russell Stothard (34), Student prizes (36),

Journals...... 37 Public Engagement and Outreach...... 40

Features: Murdoch (41) , National Science Week (42), GTAC (43), Science Meets Parliament (44), Parasites: Life Undercover exhibition (45)

Education: Concepts in Parasitology...... 49 Statistical Snapshot 2016 ...... 51 Appendix 1: Publications...... 52

Appendix 2 : Research Grants ...... 92 Appendix 3 : ASP Member Survey 2016...... 96

Page 5 Research Achievements

The contribution of parasitologists to Australia’s fundamental, With over 550 publications involving Australian parasitologists strategic and applied research effort is evident in the quantity and in 2016, there were innumerable highlights; however, 2016 was quality of publications listed in Appendix 1, all of which address especially noteworthy for Australian parasitologists’ contributions one or more of Australia’s Science and Research Priorities; thus, to the understanding of how drugs work and new insights into ASP Members contribute significantly to research on: control options for parasitic diseases, at least a few of which deserve special mention:

Environmental Change Baragaña B, Norcross NR, Wilson C, Porzelle A, Hallyburton I, Grimaldi R, Osuna-Cabello M, Norval S, Riley J, Stojanovski L, By assessing the susceptibility to, and monitoring the prevalence Simeons FR, Wyatt PG, Delves MJ, Meister S, Duffy S, Avery VM, of, parasitic disease in wildlife, ASP researchers generate new Winzeler EA, Sinden RE, Wittlin S, Frearson JA, Gray DW, Fairlamb information that will assist in the management of terrestrial and AH, Waterson D, Campbell SF, Willis P, Read KD, Gilbert IH. marine ecosystems. Discovery of a Quinoline-4-carboxamide Derivative with a Novel Mechanism of Action, Multistage Antimalarial Activity, and Potent in Vivo Efficacy. J Med Chem. 2016 Nov 10;59(21):9672-9685. Food and Water Bernigaud C, Fang F, Fischer K, Lespine A, Aho LS, Dreau D, Kelly ASP researchers work hard to: better understand the epidemiology A, Sutra JF, Moreau F, Lilin T, Botterel F, Guillot J, Chosidow O. and transmission dynamics of parasites; discover and develop Preclinical Study of Single-Dose Moxidectin, a New Oral Treatment better surveillance systems for parasites on land and in water; and for Scabies: Efficacy, Safety, and Pharmacokinetics Compared to find new ways to control parasites in our livestock and fisheries. Two-Dose Ivermectin in a Porcine Model. PLoS Negl Trop Dis. 2016 Oct 12;10(10):e0005030.

Health Creek DJ, Chua HH, Cobbold SA, Nijagal B, MacRae JI, Dickerman BK, Gilson PR, Ralph SA, McConville MJ. Metabolomics-Based ASP researchers carry out fundamental, strategic and translational Screening of the Malaria Box Reveals both Novel and Established research to: better understand host-parasite relationships; and Mechanisms of Action. Antimicrob Agents Chemother. 2016 Oct discover and develop sustainable parasite control strategies, 21;60(11):6650-6663. including new drugs and vaccines.

Images from ICTMM/ASP 2016

Page 6 Research Achievements

Garcia HH, Gonzalez AE, Tsang VC, O’Neal SE, Llanos-Zavalaga F, Gasser RB. Screening of the ‘Pathogen Box’ identifies an approved Gonzalvez G, Romero J, Rodriguez S, Moyano LM, Ayvar V, Diaz A, pesticide with major anthelmintic activity against the barber’s pole Hightower A, Craig PS, Lightowlers MW, Gauci CG, Leontsini E, worm. Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):329-334 Gilman RH; Cysticercosis Working Group in Peru. Elimination of Taenia solium Transmission in Northern Peru. N Engl J Med. 2016 Veiga MI, Dhingra SK, Henrich PP, Straimer J, Gnädig N, Uhlemann Jun 16;374(24):2335-44. AC, Martin RE, Lehane AM, Fidock DA. Globally prevalent PfMDR1 mutations modulate Plasmodium falciparum susceptibility to Goodman CD, Siregar JE, Mollard V, Vega-Rodríguez J, Syafruddin artemisinin-based combination therapies. Nat Commun. 2016 May D, Matsuoka H, Matsuzaki M, Toyama T, Sturm A, Cozijnsen 18;7:11553. A, Jacobs-Lorena M, Kita K, Marzuki S, McFadden GI. Parasites resistant to the antimalarial atovaquone fail to transmit by Wangchuk P, Giacomin PR, Pearson MS, Smout MJ, Loukas A. mosquitoes. Science. 2016 Apr 15;352(6283):349-53. Identification of lead chemotherapeutic agents from medicinal plants against blood flukes and whipworms. Sci Rep. 2016 Aug Li H, O’Donoghue AJ, van der Linden WA, Xie SC, Yoo E, Foe 30;6:32101. IT, Tilley L, Craik CS, da Fonseca PC, Bogyo M. Structure- and function-based design of Plasmodium-selective proteasome Feature stories on some of these publications, and more, are inhibitors. Nature. 2016 Feb 11;530(7589):233-6. included in the next few pages.

Preston S, Jiao Y, Jabbar A, McGee SL, Laleu B, Willis P, Wells TN,

Images from ICTMM/ASP 2016

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NEW HOPE FOR MALARIA TREATMENT mutation that protected them against the drug in early life, the mutation eventually killed the parasites by stopping production of RESISTANCE TO A KEY ANTI-MALARIAL an essential type of energy as they grew. DRUG CANNOT BE PASSED ON BY Lead authors Professor Geoff McFadden and Dr Dean Goodman MOSQUITOES IN A BREAKTHROUGH are calling it a 'genetic trap' that could prove to be a significant step forward in the anti-malaria fight. SCIENTISTS BELIEVE COULD DRASTICALLY IMPROVE THE WAY WE The pair, along with long-term collaborator Vanessa Mollard, have BATTLE THE DISEASE. THE DISCOVERY COULD POTENTIALLY SHUT DOWN THE AVENUE FOR MASS DRUG RESISTANCE TO SPREAD, MAKING MALARIA TREATMENT SIGNIFICANTLY MORE EFFECTIVE FOR THE 3.2 BILLION PEOPLE AT RISK WORLDWIDE.

The international research project was led by the University of Melbourne and focused on the drug led a team investigating the evolution and life cycle of the malaria atovaquone. parasite for the past six years.

Atovaquone was introduced in 2000 and is safe for pregnant “These results are very exciting because the spread of drug women and children, so it is one of the few anti-malarials that can resistance is currently destroying our ability to control malaria,” be used in mass administration approaches. said Prof McFadden from the School of Biosciences at the University of Melbourne. It was largely phased out of use because resistance was initially observed. “We now understand the particular genetic mutation that gave rise to drug resistance in some malaria parasite populations and how it But as published in the journal Science, the new study reveals eventually kills them in the mosquito, providing new targets for the that although some malaria parasites had developed a genetic development of drugs.”

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“So the development of drug resistance may not be a major resistance in field settings such as Kenya and Zambia. We are problem if the resistance cannot spread, meaning the drug hopeful that with the development of cheaper generic forms of atovaquone could be more widely used in malaria control. the drug atovaquone, that there is a new hope in the treatment of malaria." The team also included colleagues in Indonesia, US and Japan who have been growing and studying billions of malaria parasites used "Parasites resistant to the antimalarial atovaquone fail to infect thousands of mosquitoes. to transmit by mosquitoes", Christopher D. Goodman, Josephine E. Siregar, Vanessa Mollard, Joel Vega-Rodríguez, The researchers studied a model strain of rodent malaria and a Din Syafruddin, Hiroyuki Matsuoka, Motomichi Matsuzaki, Tomoko Toyama, Angelika Sturm, Anton Cozijnsen, Marcelo Jacobs- Lorena, Kiyoshi Kita, Sangkot Marzuki, Geoffrey I. McFadden.

Science, 15 Apr 2016:Vol. 352, Issue 6283, pp. 349-353, DOI: 10.1126/science.aad9279

http://science.sciencemag.org/ content/352/6283/349

Story source: Dr Nerissa Hannink, The University of Melbourne http://newsroom. melbourne.edu/news/new- hope-malaria-treatment-drug- resistance-found-unable-spread- first-time deadly strain of human malaria to confirm the resistant parasites Read the Pursuit article and watch video footage here: could not be spread by mosquitoes, thereby preventing the re- https://pursuit.unimelb.edu.au/articles/trapping-malaria- infection of humans. in-a-gene-net

“It is very rewarding that our fascination with basic biology has produced such significant results.”

“We are the first group to follow the drug resistant malaria parasite through its entire life cycle to understand what happens after drug resistance initially develops and whether they pass on resistance.” Images on previous page (McFadden laboratory team) and on this page “Our next challenge will be to look for any spread of this drug (mosquitoes) credit The University of Melbourne

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OPEN SOURCE DRUG DISCOVERY 2. Anyone may take part

ASSOCIATE PROFESSOR MATTHEW 3. There are no patents TODD AND COLLEAGUES RAN A Matthew Todd has been promoting open source for more than SPECIAL SYMPOSIUM ON OPEN ten years and was originally inspired by the internet and how open source was used to quickly fix any computer or software SOURCE DRUG DISCOVERY AT THE issues. “Anyone could come along and contribute to open source INTERNATIONAL software and I thought, imagine if you could do that with science,” CONGRESS FOR Matthew said. “We successfully TROPICAL MEDICINE achieved this in 2010 with an ARC AND MALARIA Linkage project with the World Health Organisation to solve a 2016 IN BRISBANE. problem to do with making the THEY EXAMINED Schistosoma drug “Praziquantel” as a single enantiomer. When this SEVERAL EXEMPLAR problem came up I thought this was PROJECTS THAT HAVE a perfect opportunity to test out EXPERIMENTED WITH the theory that open source could speed things up. The project was OPEN SOURCE DRUG successful and the problem was DISCOVERY. solved quickly because experts we didn’t know in the field of science ASP member Matthew Todd from contributed openly on the website. the University of Sydney is an These people, often from companies organic chemist coordinating the in the pharmaceutical industry, Open Source Malaria project http:// provided strategic advice and ran opensourcemalaria.org that is experiments free of charge. Science trying a different approach to curing should have an “arena” feel to it and malaria using Wikipedia, Firefox the schisto project achieved that and Linux as models for research through these outside contributors,” collaboration. The project is aimed he said. at finding new medicines for malaria using open source principles, Currently most of work involves the everything is open and anyone can synthesis of analogs of compounds contribute, embodied in the 6 Laws of Open Research, the most originally identified by big pharmaceutical companies, with the important of which are: aim of improving their potency while making the molecules more "druggable", and this is what is known as a "hit-to-lead" campaign. 1. All data and ideas are shared openly From the Open Source Malaria Landing Page scientists can see the most recent activity; there is a “To do List” and experiments from all

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contributors are recorded in the electronic laboratory notebooks. TRAUB IN CAMBODIA The Open Source Malaria (OSM) Consortium is like “Linux for Malaria Research”. REBECCA TRAUB OF THE UNIVERSITY Sharing is a fundamental theme in Matthew’s approach to science, OF MELBOURNE SPENT A WEEK IN not just with collaborators but with the general public too. He ROVEING DISTRICT, NORTHEAST believes that open science has the benefit of showing other researchers the complete research methodology, where you made CAMBODIA. mistakes and, because of the real-time nature of the project, where Rebecca Traub spent a week in Roveing District, northeast the project can go next. “In traditional research practices you don’t Cambodia training laboratory technicians for an upcoming share what your lab is doing: you would do work in the lab and then hookworm efficacy trial that is being carried out in collaboration you would distil out a certain amount of the stuff you have done with the National Centre For Parasitology, Entomology and Malaria and then publish that as a paper in a peer reviewed journal rather Control, Cambodian Ministry of Health and the Swiss Tropical like a press release,” Matthew said, “The crucial distinction (of open Medical Institute, Basel. models) is that you’re not telling people what you’ve done, you’re telling people what you’re going to do in the future.” The experience was highly enlightening for Rebecca, who feels she got her research in tropical parasitology ‘mojo’ back. OSM has grown in recent years. In 2011 the Todd lab at The University of Sydney received funding for a pilot project in open source drug discovery from the Medicines for Malaria Venture (MMV). The project champion at the outset was Tim Wells. Jeremy Burrows and Paul Willis came on board and led the suggestion to go after a few of the actives that had been placed in the public domain in 2010 by GlaxoSmithKline and others. Work got underway in the lab in August 2011. The team were successful in securing further funding from two Australian Research Council Linkage grants in 2012 and 2015. The current frontrunner compounds, in what is known as Series 4, are highly promising because two members of the series have been shown to be active in a mouse model of malaria. Matthew explained why he thinks open source drug discovery is the way forward when he said “I want a drug to be found for malaria as quickly as possible and I don’t really care who does that. The work is important, not who does it. You do it because you want to do good work, and for that to be of the widest possible use to humanity. I think the many talented contributors to the OSM consortium would agree with that.”

This article has been adapted from Tim Groenendyk’s story “OPEN SOURCE DRUG DISCOVERY” published on the University of Sydney website http://sydney.edu.au/ research/spotlight/toddosdd.shtml Above: Rebecca Traub in Cambodia

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MARSHALL LIGHTOWLERS IN NEPAL

MARSHALL LIGHTOWLERS TRAVELLED TO NEPALGUNJ IN NEPAL TO FACILITATE THE TESTING OF THE TSOL18 VACCINE.

Marshall Lightowlers travelled to Nepalgunj in Nepal as part of his involvement with the Global Alliance for Livestock Veterinary Medicines (GALVmed) which is facilitating commercialization of the University of Melbourne’s TSOL18 vaccine against Taenia solium infection in pigs. Use of the vaccine has the potential to reduce T. solium transmission and reduce the incidence of human neurocysticercosis.

In Nepal, Marshall worked with staff of the NGO Heifer, as well as students from the Institute of Agriculture and Animal Science at Tribhuvan University, to undertake detailed necropsies on 110 pigs purchased from Dalit communities in the area.

The necropsies provided baseline data on T. solium infection levels prior to implementing treatment of pigs with the TSOL18 vaccine and oxfendazole. Almost a third of the pigs were found to be infected with T. solium cysts, some with very heavy infections.

Images top to bottom: 1. Marshall Lightowlers with children in a rural area in the Nepalgunj region of Nepal. 2. Undertaking necropsy assessment of Taenia solium infections in pigs in Nepal. 3. Pork meat from an animal infected with Taenia solium 4. The lab team in Nepal.

Page 12 Researcher Exchange, Training & Travel Awards

IN 2016, 12 NETWORK RESEARCHER Md Abdullah Al Mamun, PhD student, Monash University/ EXCHANGE, TRAINING AND TRAVEL Federation University, for a 16S Metagenomic sequencing analysis AWARDS WERE PROVIDED, MOST workshop at University of Alberta, Edmonton, Canada 9-13 May 2016 GOING TO STUDENTS OR ECRS FOR VISITS TO OVERSEAS LABORATORIES Thi Hong Hanh Nguyen, PhD candidate, Bio21 Institute, for a training course Malaria Experimental Genetics, Wellcome Trust AND TRAINING COURSES. Genome Campus, UK 8 – 14 May 2016

JD Smyth Travel Award winners Sarah Preston, Post-Doctoral Researcher, Melbourne University, Gasser Laboratory for a Researcher Exchange to The University of Alexander Kennedy, PhD Candidate, WEHI, to attend XXVI Georgia, Ray Kaplan’s laboratory 12-29 February, 2016 International Complement Workshop, Kanazawa, Japan, 4-8 September and for a Researcher Exchange to Rayner group at the Michelle Power, Senior lecturer, Biological Sciences, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK. 11 - 30 Macquarie University, for a Researcher Exchange to Max Planck September Institute Berlin, Germany, to visit Kai Matuschewski to analyse Haempsporida from Australian flying fox species, August 10, 2016 Charlie Jennison (WEHI) awarded a JD Smyth Postgraduate to September 14 2016 Travel Award for to visit the University of Georgia, USA to learn the Plasmodium micro-well hepatocyte culture methods under Scott Cutmore (The University of QLD) for a Researcher Exchange Prof Kyle 26th February; Shoklo Malaria Research Unit Thailand to to explore marine trematode taxonomics with two experienced learn sample collection and P. vivax transmission; the University of scientists in Japan, Assoc. Prof James Reimer Department of Georgia, USA to infect hepatocytes and perform the downstream Biology, Chemistry and Marine Sciences, University of the processing and analysis of infected hepatocyte cultures; and to Ryukyus, Nishihara, Okinawa and Assoc. Prof Sho Shirakashi Kindai laboratory of Takafumi Tsuboi at the University of Ehime to produce University, Osaka. a number of proteins required for the generation of malarial liver stage specific antibodies. Michael Hammond (RMIT) for a Researcher Exchange to study the systematics and host-parasite interactions of bucephalid Jessica Johnson-Mackinnon (The University of Tasmania) trematodes of fishes in the laboratory of Tom Cribb, The University awarded a JD Smyth Postgraduate Travel Award to investigate the of Queensland. virulence, distribution and describe the relatedness of globally distinct outbreaks of amoebic gill disease in farmed salmon, in Hong You (QIMR Berghofer) for a Researcher Exchange to collaboration with the British Columbia Centre for Aquatic Health establish a CRISPR-Case9-mediated gene knock-down system Services, Victoria, Nanaimo and Campbell River, Canada. in Schistosoma japonicum in collaboration with Paul Brindley’s laboratory at the Department of Microbiology, Immunology and Tropical Medicine at the George Washington University USA. ASP Network Travel Award winners Javier Sotillo (JCU) for a Researcher Exchange to the laboratory of W. Deepani D Fernando, PhD student, QIMR Berghofer MRI, for Dr. Giovina Ruberti, Group leader at the National Research Council, a training course ESCMID summer school tropical and infectious Institute of Cell Biology and Neurobiology, Italy to learn techniques diseases and clinical microbiology, Seville Spain, 2 – 9 July 2016 to transfer adult schistosomes from one host into a recipient host.

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ASP Student Conference Travel Grant Technology, Sydney), Jane Kelley (La Trobe University), Kit Kennedy (University of Melbourne), Alexander Kennedy (University of The ASP awarded 108 ASP Student Conference Travel Melbourne), Weiling Koh (University of Sydney), Andrea Lawrence Grants in 2016 to attend the International Congress for (University of Sydney), Clarencia Lie (University of Sydney), Tropical Medicine and Malaria, incorporating the 2016 ASP Siew May Loh (Murdoch University), Jessica Loughland (Menzies Annual Conference, 18-22 September 2016 at the Brisbane School of Health Research), Leanne Low (Griffith University), Ann Convention & Exhibition Centre. Ly (University of Melbourne), Md Abdullah Al Mamun (Monash University), Kerryn Moore (University of Melbourne), Taylah Munro Rebecca Abraham (University of Adelaide), Mahdis Aghazadeh (Griffith University), Susanna Ng (Griffith University), Dinh Nguyen (UQ), Mohammad Al-Hasnawy (Monash University), Brendan (University of Melbourne), Chloe Nguyen (University of Sydney), Ansell (University of Melbourne), Megan Arnold (Griffith Damian Oyong (Charles Darwin University), Jacqueline Panozzo University), Jimena Balli-Garza (University of Tasmania), Amanda (Federation University), Unaiza Parkar (Murdoch University), Barbosa (Murdoch University), Priyanka Barua (University Of Kathryn Parker (ANU), Zuleima Pava Imitola (Menzies School Melbourne), Charlotte Bernigaud (QIMR Berghofer MRI), Sajad of Health Reaserch), Catherine Perez (Murdoch University), A Bhat (University of the Sunshine Coast), Jessica Bridgford Janavi Rambhatla (University of Melbourne), Mohammed Rashid (University of Melbourne), Xena Brooks (UQ), Geraldine Buitrago (University of Melbourne), Vignesh Rathinasamy (LaTrobe (James Cook University), Angela Cadavid Restrepo (ANU), Larissa University), Dhanasekaran Sakthivel (Monash university), Calarco (University of Technology, Sydney), Timothy Cameron Mengistu Seid (James Cook University), Saba Sinai-Mameghany (La Trobe University), Gowtam Chalasani (La Trobe University), (University of Sydney), Danielle Sisson (University of Melbourne), Shona Chandra (University of Sydney), Phoebe Chapman (UQ), Sarah Sloan (La Trobe University), Sylvia Squire (Murdoch Ming Chua (Griffith University), Naomi Clarke (ANU), Eimear University), Daniel Squire (Murdoch University), Aloysious Cleary (ANU), Michael Coffey (WEHI), Martha Cooper (James Ssemaganda (Griffith University), Cara Wilson (Charles Sturt Cook University), Crystal Cooper (University of Western Australia), University), Katharina Stracke (University of Melbourne), Andreas Karina De Sousa (UQ), Adelaide Dennis (ANU), Shannon Donahoe Stroehlein (University of Melbourne), Bemnet Amare Tedla (University of Sydney), Catarina dos Santos (University of (James Cooke University), Yen Thon (Thomas) Teoh (University Tasmania), Laure Dumont (University of Melbourne), Samantha of Melbourne), David Teran (UQ), Erick Tjhin (ANU), Edwin Tse Emery (Macquarie University), Jessica Engel (Griffith University), (University of Sydney), Jessie van Huis (Griffith University), Kosala E. Yagmur Erten (University of Sydney), Deepani Fernando (UQ), Weerakoon (UQ), Jessica Wilkie (La Trobe University), Isaac Kane Gillian Fisher (Griffith University), Abebe Fola (WEHI of Medical (Charles sturt University), Eleanor Steller (Charles Sturt University), Research), Camila Franca (University of Melbourne ), Hagos Luning Yang (Walter & Eliza Hall Institute of Medical Research), Gebremikael (University of Melbourne), Carlo Giannangelo Xi Zen Yap (University of Melbourne), Russell Qi-Yung Yong (UQ), (Monash University), Alexander Gofton (Murdoch University), Alireza Zahedi Abdi (Murdoch University), Joy Manyi Zeng (ANU), Rory Gough (University of Technology Sydney), Ineka Gow Meng Zhang (ANU), Bilal Zulfiqar (Griffith University). (University of Technology Sydney), Telleasha Greay (Murdoch University), Irene Handayuni (Charles Darwin University), Sanduni Hapuarachchi (ANU), Christopher Hart (Griffith University), Shihab Hasan (UQ), Chris Hosking (La Trobe University), Daniel Huston (UQ), Charlie Jennison (University of Melbourne), Jessica Johnson-Mackinnon (University of Tasmania), Fran Jones (Murdoch University), Thomas Williams (Charles Sturt University), Shilpa Kapoor (La Trobe University), Alexa Kaufer (University of

Page 14 Researcher Exchange, Training & Travel Awards

RESEARCHER EXCHANGE & TRAVEL REPORTS to analyse X-ray crystallographic data, which was kindly sponsored in part by the ASP Network Researcher exchange program. I was DHANASEKARAN SAKTHIVEL, MONASH the only student from Australia to get a boarding pass, among 16 international students selected by the committee to attend the UNIVERSITY, TRAVELLED TO JAPAN third joint Okinawa Institute of Science and Technology (OIST) FOR HANDS-ON TRAINING AT THE Okinawa Island, Japan and Comprehensive Computing suite for Protein Crystallography (CCP4), hands on training Crystallography CRYSTALLOGRAPHY SCHOOL OF School. OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY. During the first day of my stay at Okinawa Institute of Science and Technology, I had the chance to present my research findings among students and scientists from various institutions. I also "The present rate of progress [in X-ray crystallography] is gained understanding regarding the collection of X-ray diffraction determined, not so much by the lack of problems to investigate patterns of proteins in more innovative ways. In this platform I or the limited power of X-ray analysis, as by the restricted number was trained to deal with critical structure solution processes in of investigators who have had a training in the technique of the macromolecular crystallography, starting from data processing, new science, and by the time it naturally takes for its scientific and through phasing and refinement, and ending with validation technical importance to become widely appreciated." Sir Lawrence and deposition. I learned to use the next generation tools such Bragg, Australian-English physicist and X-ray crystallographer, as Mosflm, Scala, Refmac, ArpWarp, Phaser, Coot, Crank, Nobel laureate for Physics in 1915 in his concluding remark in SHELXC/D/E, Balbes, Mrbump, Buccaneer and many more Lecture (1936) popular programs used on “Forty Years of for data processing Crystal Physics” and structure solution with the team of In my PhD program, those world’s leading I aim to examine the software developers. role of carbohydrate recognizing proteins Apart from the direct of sheep (galectin help this award has 11 and 14 which given me to enable have novel anti- completion of my parasitic activities in resistant animals) and how they interact with thesis, for a graduate student like me, travelling overseas was also glycan’s present on the parasites surface in order to provide better an excellent way to expand my academic networks. Following insights in host immune resistance and for the design of a glycan the successful completion of my workshop, I travelled to Kyoto, based vaccine. In the early of 2015, I obtained the diffraction where there are thousands of ancient Buddhist temples, as well patterns of Galectin-11 of sheep and complexes of galectin-11 with as gardens, imperial palaces, Shinto shrines and traditional carbohydrate molecules for the first time. Though the X-ray data wooden houses. My international contacts certainly helped me of Galectin-11 collected, I needed appropriate training to define meet Professor Hiroshi Sugiyama's research group, members of how these host glycans interact with parasites. Hence, I attended Kyoto University. Prof’s Sugiyama group is defining the chemical the crystallography school organized by Okinawa Institute of principles underlying the recognition, reactivity and structure Science and Technology, Japan, in order to gain hands on training of nucleic acids. Here, I discussed with his Post Doctoral fellows

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Dr. Gengo Kashiwazak, Dr. Junetha Syed Jabarulla and Dr. C. progress in innovative research to develop cutting-edge diagnostic Anandhakumar of biological inorganic chemistry division, about methods and vaccine development requires greater opportunities the design of highly efficient sequence-specific DNA acting agents to work with world leaders. Indeed, my Travel to Okinawa institute and a suitable in vivo delivery method. Currently we are keen on a of Science and Technology and Kyoto University has helped me long-term goal to develop an artificial genetic switch for targeted formulate new ideas, exciting innovative ways to analyse and treatment of selected parasite diseases. understand my data and I have learnt an enormous deal. This would not have been possible without the generous support of The present drug resistant scenario in animal parasites requires Australian society for Parasitology through the ASP Researcher novel as well as more efficient approaches to improve our Exchange, Training and Travel Award. I thank the Australian understanding of host-parasite interactions and control options. Society for Parasitology for considering me for this prestigious Haemonchus award and financial contortus is a support. It’s my globally well-known immense pleasure and widespread to also extend my gastrointestinal sincere thanks to nematode (GIN) of Associate Professor sheep and goats. Fadel. A. Samatey, The parasite causes Group Leader, severe production Trans-Membrane losses due to a Trafficking Unit, range of physiologic for providing impacts including additional reduced feed intake, necessary support digestion problems, throughout the anaemia, reduced workshop. Included fecundity, poor are some pictures of weight gain, reduced me “hard at work”. milk production and increased mortality. H. contortus is ubiquitous and strongly influences livestock farm profits in tropical countries. It is estimated that 30% to 50% of production losses are caused by parasites and their treatment, where GIN infection are a significant issue. Even in the setting of Australian livestock industry where modern diagnostics and treatment are widely available, production losses and cost of drug treatment for GIN infections is Photos courtesy Dhanasekaran Sakthivel, estimated more than 1 billion dollars per annum. at the Okinawa Institute of Science and Technology (OIST) Okinawa Island, Japan Glycan and glycopeptide based vaccines are emerging candidates and Comprehensive Computing suite for Protein Crystallography (CCP4), hands on to reduce the significant burden of parasitic disease. Ultimately, training Crystallography School

Page 16 Researcher Exchange, Training & Travel Awards

ALEJANDRO TRUJILLO GONZÁLEZ, JAMES new techniques, I questioned my research approach and found better ways to accomplish my objectives during my PhD. During COOK UNIVERSITY, TRAVELLED TO SPAIN the conference, I gave an oral presentation “Tracking transparent FOR A RESEARCHER EXCHANGE AT THE monogenean parasites on fish from infection to sexual maturity” where I showed how fluorescent labelling can be used to study INSTITUTE OF AQUACULTURE “TORRE parasite ecology. DE LA SAL” (IATS) IN CASTELLÓN WITH DR ARIADNA SITJÀ-BOBADILLA AND DR. Following the conference, I started my research exchange with Dr Ariadna Sitjà-Bobadilla and Dr. Oswaldo Palenzuela in IATS, OSWALDO PALENZUELA, THIS REPORT a public research centre that belongs to the Consejo Superior BY ALEJANDRO TRUJILLO GONZÁLEZ. de Investigaciones Científicas (CSIC) in Spain. During this time I aimed to analyse samples from my current PhD project, in which I will identify strategic approaches to detect pathogens of The contribution I received from the Australian Society for quarantine concern associated with the importation of ornamental Parasitology allowed me attend to the 9th International fish to Australia. Dr Sitjà-Bobadilla is a world-renowned expert Symposium of Fish Parasites (9ISFP) and a month long research of myxozoan, coccidian and monogenean parasites of farmed exchange in the Institute of Aquaculture “Torre de la Sal” (IATS) in fish and Dr. Palenzuela is a specialist of Cryptosporidium and Castellón, Spain, from the 1st of September to the 12th of October. Enteromyxum species infecting marine fish. The 9th ISFP was held at the University of Valencia Campus, where researchers from all over the world presented ground- During my month working in the Institute, I was trained in breaking research molecular and in various areas of morphological fish parasitology. identification of As a first year PhD myxosporean and student, this was a cryptosporidian unique opportunity parasites. I also to meet new learned techniques people and learn to maintain an about what its in vivo culture of currently being Enteromyxum leei done in research infecting gilthead laboratories around the world. As I meet more researchers and sea bream, Sparus aurata, as part of ongoing experiments in the facilities of the Institute. Specifically, I learned to develop nested PCR protocols to identify Cryptosporidium species Above left: working with a gilthead in samples collected from infected fish. I honed my skills in seabream, Sparus auratus infected with histopathology and learned to identify myxozoan, myxosporean myxozoan parasite, Enteromyxum leii. Above right: Aquatic Pathology Labora- and cryptosporidian parasites found in different fish organs. tory team. From Left to right: Dr Oswaldo Palenzuela, Alberto Fernandez, Raquel Raquel del Pozo (Senior lab technician) was invaluable in my del Pozo, Dr Ariadna Sitjà-Bobadilla, Inmaculada Vicente, Nahla Hossam and understanding of the nested PCR protocols and Dr Carla Piazzon Eldin Ahmed. (PostDoc researcher) helped me to identify potential issues in

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PCR protocols and what stages could be modified to increase the ALI RAZA OF THE UNIVERSITY OF sensitivity of the protocols. Inmaculada Vicente (Senior animal QUEENSLAND TRAVELLED TO CANADA husbandry assistant) and Alberto Fernandez (lab technician) showed me the re-circulating and flow-through systems they had FOR A RESEARCHER EXCHANGE AT established to maintain different species of fish, and what stages THE UNIVERSITY OF MONTREAL AND were of critical importance to maintain experimental infections and avoid cross-contamination between tanks. This knowledge greatly THEN ON TO SAN DIEGO, FOR AN increased my understanding in animal husbandry and gave me ANTHELMINTICS WORKSHOP. essential skills I will use later in my PhD. I had the opportunity to spend about 4 weeks in Prof. Roger Most importantly, I learned how Prichard’s laboratory at the Institute of to cure and prepare olives for Parasitology, McGill University, Montreal, consumption. This was a small side Canada, in early 2016. I received a warm project I had during my spare time, welcome by the freezing cold of Montreal and as Rosario (the gatekeeper of the when I landed there. I was very excited to Institute) said: “olives are an essential be making this trip because this laboratory component of Spanish culture”. is one of the world’s leaders in research into anthelmintic resistance. The work carried out I would like to thank the ASP for in this lab is well aligned to my own interests. their funding and encouragement The people working in the institute were very of students to seek out international friendly and cooperative, and they cordially opportunities. My research exchange welcomed me to visit their labs. My main established the first collaboration purpose was to learn some techniques in between James Cook and IATS, proteomics and genomics as Prof. Prichard’s paving the way for future exchange group works on drug transport proteins, ion students to have more opportunities channels and drug targets in nematodes and to travel and get involved in other their roles in anthelmintic resistance. research projects. With the help of the APS and IATS in Spain, Australian During my visit, I was invited to not only students have the opportunity to attend the weekly seminars in the institute develop research in Europe, with and hear about the on-going projects new techniques, new fish species, by different research groups, but also to and innumerable opportunities to present my research work and promote learn something new. the Australian research on anthelmintic resistance. In addition, I also had an opportunity to work on a high-throughput technique, “pyrosequencing” to measure the frequency of single nucleotide polymorphisms (SNPs) in resistant Above, Alejandro and friends at the 9th and susceptible isolates of Haemonchus contortus collected from ISFP in Valencia, Spain from left to right: different sheep farms across Canada. This project is focusing on Kate S. Hutson, Ana Delgado, Alejandro detection of the SNPs in beta-tubulin and dyf-7 genes responsible Trujillo, Alexander K. Brazenor at the 9th ISFP in Valencia, Spain. for resistance to benzimidazole and ivermectin in H. contortus.

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Using this technique, we can investigate and identify the possible valuable addition to my career portfolio. markers that can be used to diagnose nematode population resistant to anthelmintics in field conditions. I also attended a short During my visit, I also participated in an international symposium training session on basic bioinformatics and the use of software for entitled “Anthelmintics: Discovery to Resistance II” held in phylogenetic analysis. San Diego, CA, USA, and presented my research work. I had good discussions on future research directions in the area of Prof. Tim Geary, the director of the institute is a great personality, anthelmintic resistance with leaders in my field of research. In who allowed me to work in his lab where I learned about the addition, I also discussed on some future postdoctoral career use of fluorescent microscopy to investigate the effects of opportunities with different research groups flubendazole on the cuticle of Brugia malayi. Meghan O’Neil (a PhD student) also helped In short, my visit to me to differentiate between the institute and drug anatomical structures resistance symposium, of this nematode and albeit for few weeks, was understand its handling highly successful and most and storage. Researchers enjoyable. I really enjoyed in Prof. Geary’s group are the chilling weather of also working on associating Montreal, company of my nematode ion channel colleagues and the new activity with anthelmintic networks that I created. In sensitivity. Different drug concluding, target proteins (glutamate- gated chloride ion channels I would like to acknowledge and nicotinic acetylcholine Prof Roger Prichard, Prof receptors) are expressed Tim Geary and all the in Xenopus oocytes and staff and students in the the effects of drugs on institute especially Kathy opening and closing of the Keller (senior technician) ion channels are studied for their cooperation and in a voltage-clamp technique. I was fortunate to learn about this amazing hospitality throughout my stay. Furthermore, I would technique and also assisted in a surgical procedure to harvest the especially extend my gratitude to Mr. Shoaib Ashraf (ex-PhD oocytes from frogs. It was quite interesting for me to inject RNA in student from the lab) for his wonderful hospitality and kind these tiny round ‘balls’ (oocytes) using a microscope and very fine cooperation in arranging my accommodation and welcoming microneedles. me to his house for delicious meals quite often. I successfully represented the ASP and the University of Queensland, and I also participated in the actual electrophysiology experiments promoted Aussie research on anthelmintic resistance in Canada performed using a well-established system. I was also interested and the USA. I am greatly thankful to the ASP and the Graduate to learn the protein modelling and Mark (a PhD student) helped School, University of Queensland for the financial support that me to understand the basics of protein modelling and the use of allowed this fruitful visit to take place. different software for this purpose. The working experience on such advanced techniques which I acquired during this visit is a Image courtesty of Ali Raza

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HANH NGUYEN FROM THE UNIVERSITY course. Marcus Lee guided me to design CRISPR single-stranded OF MELBOURNE WAS AWARDED AN ASP guide RNA and I successfully incorporate it into one of his pre- NETWORK RESEARCHER EXCHANGE, designed vector. This vector will be used as part of my project to generate edited bromodomain protein lines. Recently, I returned TRAINING AND TRAVEL AWARD TO to my laboratory and aimed to set up an adaptable, efficient and ATTEND A TRAINING COURSE IN easily operated CRISPR system for all of our members.

MALARIA EXPERIMENTAL GENETICS In addition, the course also focus on performing and comparing AT THE WELLCOME TRUST GENOME the difference between different eletroporation systems when CAMPUS, UK IN MAY 2016 working with P. falciparum, P. berghei and P. knowlesi. In my laboratory, we routinely use Biorad electroporation system for P. falciparum system which is cheaper to operate with relatively I attended the Malaria Experimental Genomics course at the decent yield. However, the Biorad is inefficient for experiments Wellcome Trust Genome Campus in Hinxton from 8th May to 14th requiring multiple transfections. The Lonza (Amaxa) is better May 2016. suited for this purpose as there is a plate format that can hold up to 96 transfections where each condition requires less input The course was conducted by Julian DNA. The electroporation is also Rayner, Oliver Billker and Marcus gentler on the cells which may lead Lee. The course took place at the to better transfection yield. My CL1 and CL3 teaching laboratory laboratory is exploring Amaxa as an at the Sanger Institute under the alternative option. Bioinformatic supervision of the demonstrators. analysis such as RNA-sequencing The course covers gene editing analysis visualisation was also methods, malaria parasite explained during the course by transfection, genotyping, flow Dr. Thomas Otto. Everyone was cytometry and RNA-sequencing also provided opportunity to visit analysis visualisation. There were a loader, whose job is to set up the presentations from international sequencing machine, and show invited speakers from the United us that Sanger Institute is truly a States, Europe and Australia as well sequencing leading centre. as local speakers. While the topic discussed was broad, the focus revolves around the discussion of the conditional knockdown My project aims to generate bromodomain protein knockdown system used for different settings and experimental purposes. parasite lines to test the influence of bromodomain inhibitors on them. Dr. Marcus Lee is funded by the GSK to generate The talks were particularly informative as students were provided bromodomain protein knockout parasite lines with similar chances to ask questions and discuss specific projects with the purpose; and through the course, we discussed the project where speakers during the talk or over the next few days of the course. he suggested some insights that I will try incorporate into my project. Gene editing using CRISPR was one of the major themes for the It was a great place to study and talk to other experts in the field. I Above: course attendes at Hinxton am most grateful to the ASP for providing me with this opportunity.

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USING HIS JD SMYTH POSTGRADUATE unpublished work from my PhD candidature. The abstract for this TRAVEL AWARD, ALEXANDER KENNEDY talk has been published in the journal Immunobiology, Volume TRAVELLED TO KANAZAWA IN JAPANA 221(10) pp. 1155. My talk was well received with numerous questions and comments from laboratory heads in the field of AND HINXTON AND LONDON IN THE UK complement and complement regulation on a range of topics BETWEEN 2ND SEPTEMBER AND 3RD OF around Plasmodium falciparum interactions with the complement OCTOBER 2016. system. This workshop also offered a teaching day for young investigators I would first like to thank the Australian Society for Parasitology that I attended. It provided a comprehensive background of for their generous support. As outlined below this travel was the complement system in the morning followed by themed exceptionally productive, providing new ideas and collaborations sessions in the afternoon. I elected to attend the ‘Therapeutic as well as laying the groundwork for successful P. knowlesi Intervention in the complement system’ and ‘Complement culturing in Melbourne. deficiencies and diseases’ sessions. Both were led by experts in their field and provided a great deal of insight into how complex 26th International Complement Workshop; 03/09/2016- and interconnected the complement system is as well as ways we 09/09/2016; can intervene in Kanazawa, Japan this system. I look forward to applying I attended the this knowledge to 26th International how Plasmodium Complement falciparum Workshop held in interacts with Kanazawa, Japan. and dysregulates This workshop the complement occurs every two system. years, bringing together experts in Furthermore, the field of complement, a central component of innate immunity. attendance at this meeting led to new collaboration opportunities, with a leading European complement laboratory making unique The abstract that I submitted to this conference was accepted for a reagents available that will allow further investigation of host- 15-minute oral presentation. My presentation was titled ‘Hijacking pathogen interactions we have identified. Additional discussions host complement regulators: mechanisms of Plasmodium also offered new avenues of deeper inquiry into our current falciparum complement evasion’ and covered both published and projects.

Researcher Exchange: Wellcome Trust Sanger Institute, Rayner Group 11/09/16-23/09/16 Above left: Kenrokuen Garden, Kanazawa, Credit: 26th International Complement Workshop. Following the conference, I went to the laboratory of Julian Rayner at the Wellcome Trust Sanger Institute in Hinxton, United Above right: The Wellcome Trust Sanger Institute in Hinxton, UK. Kingdom. The purpose of this visit was to learn to culture the

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simian malaria parasite P. knowlesi, which has been adapted In conclusion, the two weeks at Sanger Institute were highly to invade human red blood cells. P. knowlesi causes zoonotic productive, covering the essentials needed to establish P. knowlesi infections in South-Eastern Asia. Initially, I was taught to maintain culturing and experiments in Australia. This success is thanks to a culture of P. knowlesi and how to identify the stages and Julian Rayner and the welcoming and helpful attitude of his lab morphology of the parasites during their rapid 27 hour lifecycle. members. In particular, I would like to thank his postdoctoral fellow In addition, I learnt to purify the late stage P. knowlesi schizonts Dr Theo Sanderson who supervised and taught me all of the P. via density method using a nycodenz cushion and how to use knowlesi techniques mentioned above. these schizonts to produce a synchronous P. knowlesi culture. This method was particularly valuable as generating a synchronous Researcher Exchange: London School of Hygiene and Tropical culture is important for Medicine, Moon Group downstream assays. In 26/09/16 – 03/10/2016 addition, P. knowlesi is resistant to sorbitol I also visited Dr Robert lysis, a common method Moon, who established used for generating the human adapted P. synchronous P. knowlesi cell line, at his falciparum cultures. In laboratory at The London addition, I was taught to School of Hygiene and successfully freeze and Tropical Medicine for one thaw P. knowlesi cultures, week. While at LSHTM, which will be important I presented a public for establishing these seminar of my work on P. cultures in Australia. falciparum complement evasion strategies. In My P. knowlesi addition, I had extensive synchronous parasites discussions on the P. were used to set up knowlesi culturing methods a growth inhibition. refining the knowledge These assays produce I had earlier learnt at the key functional data about parasite viability in different conditions. Sanger Institute, as well as receiving detailed protocols for assays In this case, we tested the IC50 of several known P. falciparum such as immunofluorescence assays. Dr Moon has also been inhibitors that were at hand, gaining interesting preliminary data able to transfect P. knowlesi with exceptional efficiency and I was worth further follow up in the future. able to observe the methods used to prepare and perform these high efficiency transfections. This was a very productive week of I was also guided through a transient transfection protocol that research exchange, owed in great part to Dr Moon’s generosity would express luciferase off and episomal plasmid in P. knowlesi. with his time and methods. Our transfection was a success with luciferase activity readily detected the following day. Being able to successfully transfect P. knowlesi will allow us to produce transgenic P. knowlesi lines here in Australia allowing us to further probe the parasite biology. Above: Plasmodium knowlesi schizonts growing in human red blood cells stained with Giemsa at 100 x magnification. Schizonts were enriched by floatation on a nycodenz cushion (right).

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KATE HUTSON DESCRIBES HER nearly every animal group, ranging from sponges to chordates. EXPERIENCE OF THE INTERNATIONAL Only about 2% of aquatic invertebrate species and less than 20% WORKSHOP ON SYMBIOTIC of fishes have been surveyed for symbiotic copepods, which means there are many more waiting to be discovered and formally COPEPODA, HELD AT THE HERON described. ISLAND RESEARCH STATION, A TRIP The 1st and 2nd IWOSC were held at Cabrillo Marine Aquarium MADE POSSIBLE BY THE SUPPORT OF in San Pedro, California, U.S.A. and the University of Limpopo THE ASP NEWTORK'S TRAVEL AWARD in South Africa, respectively. This 3rd IWOSC at Heron Island SCHEME. comprised a fully residential event including Researchers research and students presentations, from around the laboratory based world meet every workshops and three years to the opportunity exchange ideas for participants to and learn research collect workshop techniques related specimens on symbiotic on field trips in copepods during the scientific the International research zone of Workshop the Great Barrier on Symbiotic Reef Marine Copepoda Park. Four days (IWOSC). This year were dedicated the conference to symbiotic was held at Heron Copepoda, with Island Research the final day Station, Australia on symbiotic and was generously supported by the Australian Society for Isopoda. The workshop was organised by Kate Hutson (James Parasitology. The global shortage of technical expertise in copepod Cook University, Australia), Julianne Kalman Passarelli (Cabrillo taxonomy is a threat to biodiversity studies and aquatic animal Marine Aquarium, USA) and Danny Tang (Orange County health industries. More than 11,000 valid copepod species are Sanitation District, USA) while guest tutors included Geoff Boxshall known, about half of which live in symbiotic associations with of the Natural History Museum, London, Rony Huys of The Natural History Museum, London, Nico Smit of North-West University, South Africa and Niel Bruce of the Queensland Museum, Australia.

The first day began with a welcome and introduction session and Above: Jade Morris on snorkel during collections in the scientific zone off Heron Island, Great Barrier Reef (Photo: K Morris) a plenary presentation by Geoff Boxshall with an introduction to

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parasitic crustaceans. Following we were given and introduction following day. to copepods of invertebrates by Rony Huys and the history of the discovery of Australian marine parasitic copepods by Kate Hutson. The weather closed in on the fourth day which suited our planned In the afternoon we heard from Jimmy Bernot, Diane Alps, Bruno lectures on copepod life cycles, how to examine copepods Passarelli, Alejandro Trujillo-González, David Vaughan, Jonathan specimens, illustration techniques and the use of dichotomous Barton, Nico Smit keys. After lunch we and Julian Uribe- were challenged Palomino on their with a laboratory research involving a activity where diversity of aquatic participants were parasite fauna. given preserved material and the The second and third resources to key days were themed out the specimens with a focus on to family and symbiotic copepods genus. The final day of invertebrates and involved a lecture fish, respectively. by Nico Smit and a Collections on the workshop by Nico reef were made and Niel working in the mornings with keys and fresh followed by lectures and preserved by the organisers parasitic isopod and tutors on specimens. preservation, identification Unfortunately the and accession weather continued techniques before we moved into the laboratory to dissect host to deteriorate and the 3rd IWOSC participants found themselves specimens and stranded on Heron Island in gale force winds and rain! Fortunately examine fresh our caterer Pat kept us well fed, Niel kept us entertained with preparations highlights from Africa and the wind subsided enough for us to of symbiotic return to Gladstone the following day! copepods. In the evening seine netting under the stars Top: Organisers and guest tutors (L-R): Nico Smit, Niel Bruce, permitted Danny Tang, Rony Huys, Julianne Kalman Passarelli, Geoff Boxshall and Kate Hutson (Photo D Alps). Middle left: nspecting collection of invertebrate host collections for copepods (L-R): Alejandro some nocturnal Trujillo Gonzalez, Danny Tang and Jimmy Bernot. Middle right: fishes for each afternoon there were opportunities to examine fresh and preserved material in the laboratory. Bottom: tellicola sp. in inspection the starfish Nardoa cf tuberculata (photo J Bernot)

Page 24 Honours

Bancroft Mackerras Medal • The SHIFT Team, a collaboration between Murdoch Childrens Research Institute, The Kirby Institute, St Vincent’s Hospital, Professor Denise Doolan of the Berghofer Queensland Institute Menzies School of Health Research and the Fiji Ministry of of Medical Research was awarded the Bancroft Mackerras Medal Health that has produced a landmark study showing that for 2016. Denises's citation is reproduced on the following pages. mass drug administration with the oral drug ivermectin is highly effective in controlling scabies and related bacterial Australia Day Honours skin sores. SHIFT has transformed the global conversation on integrated programs for neglected tropical diseases. (Finalist Congratulations to Dr Peter Anthony Holdsworth AM, Wanniassa, in the Australian Infectious Diseases Research Centre Eureka ACT. Peter was recognised for distinguished service to veterinary Prize for Infectious Diseases Research category). science, particularly to animal parasitology and pharmaceutical development, and to professional scientific organisations. • Professor Miles Davenport, Dr Deborah Cromer, Dr Mykola Pinkevych and, Dr David Khoury, Kirby Institute, Georgina Sweet Award UNSW; Professor Stephen Kent, Peter Doherty Institute, The University of Melbourne; and Dr Ashraful Haque, Associate Professor Freya Fowkes of the Burnet Institute won the QIMR Berghofer Institute for their unique integration of prestigious 2016 Georgina Sweet Award for Women in Quantitative mathematicians, computer scientists and physicists, in close Biomedical Science. Freya is featured on the pages that follow. collaboration with experimental scientists and clinicians, to develop a novel understanding of the ‘mathematics of Eureka Prizes infection’. The team has used these insights to design and optimise treatment and vaccination for major infectious Continuing a fine winning tradition for parasitology at the diseases. (Finalists in the UNSW Eureka Prize for Excellence in prestigious Eureka Prizes, Professor Leann Tilley, Dr Nick Klonis, Interdisciplinary Scientific Research category). Associate Professor Julie Simpson and Associate Professor James McCaw, from The University of Melbourne, took out the Australian Griffith University Infectious Diseases Research Centre Eureka Prize for Infectious Diseases Research for their key scientific discoveries leading to Professor Vicky Avery received the 2016 Pro Vice Chancellor's insights into how artemisinin resistance by the malaria parasite Research Excellence Award - Mid/Senior Career Researcher. may be overcome. Dr Melissa Sykes was awarded the 2016 Pro Vice Chancellor's Research Excellence Award - Early Career Researcher Our congratulations also to several other finalists for the 2016 awards including:

• The Boddey, Sleebs and Cowman team, Walter and Eliza Hall Institute of Medical Research, in the Australian Infectious Diseases Research Centre Eureka Prize for Infectious Diseases Research category for their research into determining how malaria parasites use a unique protein export pathway across the parasite’s lifecycle, enabling collaboration with industry that is accelerating the development of a new antimalarial drug.

Page 25 Honours

RECOGNITION FOR ASP MEMBERS With the award Associate Professor Fowkes will aim to understand how elimination of malaria can impact on rebound of clinical disease, and elucidate how infectious malaria reservoirs can spread drug ASSOCIATE PROFESSOR FREYA resistant malaria. FOWKES OF THE BURNET INSTITUTE WON THE PRESTIGIOUS She said it was a great honour to receive the Georgina Sweet Award, also won by Associate Professor Alicia Oshlack, Murdoch Childrens 2016 GEORGINA SWEET AWARD Research Institute and Associate Professor Kathryn Holt, University of FOR WOMEN IN QUANTITATIVE Melbourne.

BIOMEDICAL SCIENCE “To be recognised as a female leader in science in Australia is a great honour and I Head of Burnet hope to emulate Institute’s the success and Malaria and achievements of Infectious Disease other inspirational Epidemiology female scientists Group, Associate such as Professor Professor Tilley” Associate Freya Fowkes Professor Fowkes (pictured) won the said. prestigious 2016 Georgina Sweet “During the award Award for Women I hope to act as an in Quantitative ambassador for Biomedical women in science, Science. The promoting and $25,000 award inspiring emerging is presented to female leaders outstanding mid- in science in career female Australia.” scientists across Australia who Associate Professor demonstrate Fowkes received excellence in the area of Quantitative Biomedical Science. her award at a ceremony on Thursday 27th October at Melbourne University. The Georgina Sweet Award for Women in Quantitative Biomedical Science was created by Professor Leann Tilley as part Story and picture courtesy of the Burnet Institute of her Australian Research Council Laureate Fellowship program, https://www.burnet.edu.au/news/739_burnet_malaria_ to promote and support female scientists who demonstrate researcher_wins_georgina_sweet_award excellence in the area of Quantitative Biomedical Science.

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BANCROFT-MACKERRAS MEDAL 2016 have appeared in the very best journals, including Science, Nature Med, Immunity, PNAS and Journal of Experimental Medicine. IT GIVES US ENORMOUS PLEASURE TO ANNOUNCE PROFESSOR DENISE Denise conducted her PhD at University of Queensland/ Queensland Institute of Medical Research (QIMR), where she was DOOLAN OF THE BERGHOFER supervised by Professor Michael Good, and became his first PhD QUEENSLAND INSTITUTE OF MEDICAL graduate. Denise then moved to arguably the best malaria vaccine RESEARCH AS THE BANCROFT laboratory in the world, The Naval Medical Research Centre in MD, USA, working under the tutelage MACKERRAS MEDAL of Professor Stephen Hoffman. (BMM) RECIPIENT While at the naval malaria labs, Denise rapidly rose through the FOR 2016. civilian ranks to become director of basic and pre-­clinical research, Denise’s contributions to where she led numerous malaria Australian parasitology are well vaccine development projects and and truly encompassed within clinical trials and developed an the spirit of the BMM. Denise’s international reputation that was dedication to, and unwavering second to none. It was therefore support of, the ASP is evident to a great coup for QIMR in 2007 to all. She was recently president lure Denise back to Australia as of the society, presiding over a group leader on a prestigious an industrious period of growth Pfizer Research fellowship. Since for the society, which included her return to Australia, Denise expansion of the IJP family of has established the Molecular journals, conceptualization of the Vaccinology laboratory at QIMR-­ postgraduate course (Concepts in Berghofer and currently heads Parasitology), and many other new the Biology program at the initiatives. While steering the ASP institute. After completing her through one of its most exciting Pfizer fellowship, Denise entered development periods, Denise also the highly competitive NHMRC made outstanding advances in the fellowship scheme as a Principal fields of molecular immunology Research fellow in 2013. and vaccinology of malaria. Over her career Denise has published Denise has attracted an well over 100 papers in the astonishing amount of funding for peer-­reviewed literature, 50 of her research on malaria vaccines. these in the last 5 years. Moreover, Many Australian researchers many of these papers where struggle to maintain their Denise is first or senior author productivity after working in the

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vibrant and well-­‐ funded critical mass of researchers in the US. malaria adenovirus vectored vaccines, showing that they could Denise, however, not only managed to obtain NHMRC program simultaneously induce both T cell and antibody responses and and project grant funding, but also enjoyed the benefits of being protect against parasite challenge, and could be designed to principal investigator on grants from the US National Institutes co-­express multiple target antigens. Her team also conducted of Health. This is no mean feat for a non-­US researcher working the seminal study demonstrating that adenovectors can induce in Australia, and as a sole-­investigator no less! Her research has functional antibodies capable of potent inhibition of blood-­‐stage led to a number of paradigm shifts in fields of vaccinology and malaria parasite growth, with levels exceeding those induced immunology, and this is reflected in not only her publications by protein in adjuvant. but the 13 patents on which she is an inventor in the field of infectious disease vaccines. Among her awarded patents is one Denise’s overall scientific accomplishments are numerous. In the arising from her demonstration that an antigen expressed during past 5 years however, key advances that she has pioneered are the liver but not sporozoite stage of the Plasmodium life cycle is a in the area of systems immunology. Denise was the first to apply target of sterilising protection! Previously, the liver-­stage had been the growing technology of proteome microarrays to Plasmodium, considered protected from host immune responses, and it is this and used this information to search for the best malaria vaccine sort of out-of-­ ­the-­box thinking, with no “dogmatising” restraints, antigens using a high-­throughput approach. She has since that typifies Denise’s approach to her research and accounts exploited this technology to reveal an entirely new set of T and B for her reputation as a thought-leader­ and pioneer in this area. cell antigens for inclusion in malaria vaccines that have until now Indeed, when the editor-­in-­ chief of Nature Genetics, a journal that gone completely unnoticed. Using a rational approach to high-­ needs no précis, recently visited her institute, he made a strong throughout screening, and combining human and mouse studies, recommendation that Denise submits her most recent work to Denise has addressed the complex nature of protective immunity one of the leading Nature journals, emphasising that we have in malaria through meticulous dissection of the protective immune not yet seen the best of Denise Doolan! Having seen Denise response at the molecular level. present the work in question over the last few years, I have no doubt that her next series of publications will have an even bigger Not satisfied with merely identifying the major antigens that impact on the field of malaria vaccinology than he current work, are required for an efficacious vaccine antigen, Denise has and perhaps reinvigorate this important area of endeavour that made major discoveries in understanding the nature of the has suffered recently due to bad publicity around a sub-­optimal protective immune response to malaria. Through genuine vaccine that is in the final stages of human clinical trials. scholarship and an unparalleled knowledge of malaria biology and vaccinology, she has demonstrated that humans recognize In the area of vaccinology, Denise provided the first experimental CD8+ cytotoxic T lymphocyte epitopes on the Plasmodium verification of the concept of multi-­valent vaccination for any falciparum circumsporozoite protein, the core component of pathogen with any vaccine delivery system, resulting in a paradigm the most advanced malaria vaccine candidate RTS,S. Denise shift in strategies for vaccination against tropical diseases. She then went on to show that cytokines are the critical mediators of played a key role in the evaluation of DNA vaccines as a promising immunity against liver stage malaria, resulting in a paradigm molecular vaccine platform, demonstrating that DNA vaccines shift for pre-­erythrocytic malaria vaccine design. This marriage against malaria are immunogenic in mice and monkeys, and of antigen discovery and detailed immunologic mechanisms of executing the clinical immunology in support of the seminal protection resulted in development of a novel epitope-­based T study demonstrating the safety and immunogenicity of DNA cell screening strategy to rapidly identify target antigens of T cell vaccines in healthy humans. This established the foundation responses from genomic sequence data. for clinical testing of DNA vaccines against other pathogens. She also led the preclinical evaluation and development of The outcomes and relevance of Denise’s research outside of

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the malaria field are evident. She is responsible for the first and postdocs, and a valued senior member of the QIMR Berghofer experimental verification in any system of the concept of HLA executive. In terms of this BMM nomination, Denise’s contributions class I supertypes, demonstrating that epitopes predicted to the field of parasitology at large have been utterly selfless and by computer algorithms can be generated in vivo during the ambassadorial, typified by her leadership of the ASP, her editorial course of natural infection and recognised by host T cells. She and reviewer contributions to journals in the parasitology field, and subsequently extended this paradigm to class II T cell responses. the quality and quantity of her research output. These discoveries established the feasibility of developing a universally effective vaccine by focusing on a limited number of In 25 years of sustained research, Denise Doolan has proven herself peptide specificities. This new concept of HLA supertypes has to be a leading light in the malaria research community. The impact now been embraced by researchers in diverse fields including HIV, of her past, current, and undoubtedly future research efforts will cancer, and autoimmunity, and has provided the foundation for a lead to better treatments and control of malaria, and ultimately an public access database and analysis resource for epitope data for efficacious vaccine. infectious diseases, allergens and autoimmune diseases. Congratulations, Denise, winner of the Bancroft-Mackerras To summarise, Denise Doolan is an internationally recognised Medal, 2016. authority in malaria vaccine discovery and development. She is at the top of her game, and is on the cusp of making some of her greatest discoveries to date. She is a prolific author of high quality publications, a mentor held in the highest regard by her students

Images from ICTMM/ASP 2016

Page 29 ASP Annual Conference

The 2016 ASP annual conference was part of the XIX International Congratulations to Malcolm Jones and the Management Congress for Tropical Medicine and Malaria 2016 (ICTMM2016) Committee (Denise Doolan, Kathy Andrews, David Looke, James and jointly hosted by the Australasian Society for Infectious McCarthy and Paul Griffin) for a wonderful conference with an Diseases (ASID). exciting program featuring ASP Invited Lecturers Professors Russell Stothard, Peter Crompton and Maria Dolores Bargues ICTMM2016 delivered workshops, satellite meetings and 83 together with IJP invited lecturer Professor Leann Tilley, IJP:PAW concurrent sessions to 1500 delegates. With many thanks to invited lecturer Professor Dr Thomas Romig and IJP:DDR invited supporters and sponsors of ICTMM2016; IJP and Elsevier, , lecturer Professor David Fidock. Georgina Sweet Travel Support from Melbourne University and the Boehringer Ingelheim Foundation. The ASP will adopt and The conference started with some free parasitology fun on Sunday make changes to the Gender Equity policy that was developed as 18 September 2016 at the ICTMM Opening Ceremony in Brisbane part of the Georgina Sweet Travel Support. Many thanks to The with four of our most illustrious and entertaining scientists Peter Bill & Melinda Gates Foundation who provided funding for Awards O’Donoghue, Michael Good, Charlene Willis, and David Jenkins for Talented Tropical Medicine and Malaria Investigators from presenting about topical tropical medicine, the convoluted Resource-Poor Regions to Attend and Participate in ICTMM 2016. evolution of malaria vaccine strategies, and the many weird and wonderful parasites and the scientists who love them.

Invited Lecturers at ICTMM 2016: Top Row: David Fiddock, Leann Tilley and Maria Dolores Bargues

Invited Lecturers at ICTMM 2016: Botton Row: Russell Stothard, Peter Crompton and Thomas Romig

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Opening Ceremony

The conference started on Sunday 18 September in Brisbane with parasitology presentations by four of our most illustrious and entertaining scientists Peter O’Donoghue, Michael Good, Charlene Willis, and David Jenkins.

Images from ICTMM/ASP 2016

Page 31 Above and on next page: Images from the 2015 joint ASP- NZSP Conference in Auckland

ASP Annual Conference

ECR Breakfast

ASID & ASP co-sponsored the Early Career Breakfast on Monday 19th September 2016. 168 ECRs enjoyed networking and meeting some of our invited speakers, Leann Tilley, Una Ryan, Malcolm Jones and Cheryl Jones, ASID President. Nick Smith hosted the event.

Images from ICTMM/ASP 2016

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ASP Annual General Meeting

The 2016 AGM was a lively meeting attracting 120 ASP members to the Queensland Museum on Tuesday September 20. Members had a chance to explore the fabulous exhibition Parasites: A Life Undercover, which is sponsored by the Society.

Images from ICTMM/ASP 2016

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AN INTERVIEW WITH RUSSELL STOTHARD introduction to why he chose this topic. It is actually just over 100 years ago that the lifecycle of the African schistosome was PROFESSOR RUSSELL STOTHARD FROM described by R.T. Leiper and it’s important to remember its today. This is framed within a countdown on WHO 2020 targets for THE LIVERPOOL SCHOOL OF TROPICAL neglected tropical diseases, with COUNTDOWN an acronym of the MEDICINE WAS AN ASP INVITED DFID, UK programme he directs (see www.countdownonntds.org). INTERNATIONAL LECTURER IN 2016. Despite control, schistosomiasis is a major scourge to the health LISA JONES SPOKE WITH HIM AT ICTMM of children throughout Africa and there is urgent need to scale-up AND SPOKE TO HIM ABOUT HIS WORK. preventive chemotherapy. Although Leiper was a clinician, from the London School of Tropical Medicine and Hygiene, his passion Professor Russell Stothard from the Liverpool School of Tropical was in solving conundrums, parasite lifecycles and having worked Medicine was an ASP Invited International Lecturer in 2016. on other worms, those of fish, his acumen and insight was very well honed. In addition to presenting at the 2016 ASP Conference at ICTMM in Brisbane, Russell visited Robin Gasser’s laboratory at the Russ’s tongue-in-cheek remark is also a friendly pinch at clinical University of Melbourne and Don McManus’ laboratory at colleagues in the BSP who had been dismissive of those with QIMR Berghofer MRI. This was interests in wildlife, which ultimately his first visit to Australia but he led to a split of malariologists from the has corresponded with several Spring Meetings, a dichotomy that Australian colleagues throughout began in the late 1980s. Russ remarked his career. Russ has always admired that it has taken a long time to get the ASP, for example, one of his these factions working together again, earlier papers was published united mainly by the rise in molecular in the International Journal for epidemiology with shared laboratory Parasitology on Trypanosoma tools and computer approaches that cruzi and most recently this year simply didn’t exist a decade before. on female genital schistosomiasis. Until last Easter, Russ was Honorary Like Leiper, Russ is firmly interested in General Secretary of the British OneHealth and being brought up on Society for Parasitology (BSP), the a farm in the rural borders of England members of which have diverse and Scotland he was interested in interests from medical to molecular etc., and he was keen to see parasites well before becoming a researcher. He developed a how ASP compared to the BSP. fascination of diseases in livestock, their ecology and also became a keen angler catching anything that could swim, or finding things At the ICTMM, I caught up with him and asked why he chose within things that swam! Russell studied Microbiology and Zoology to present on COUNTDOWN and Leiper’s lasting legacy at Leeds, and after an expedition to East Africa became interested in helminthology (see http://blog.journals.cambridge. in molluscs and their snail-borne diseases. org/2016/09/05/robert-t-leipers-lasting-legacy-on- schistosomiasis/). “Scratch the surface of a human parasitologist In 1989 he took part in marine surveys, piloting small boats across and you’ll find a fish parasitologist waiting to get out” was Russell’s the coral reefs of Mafia Island, Tanzania. Some 25 years later,

Page 34 ASP Annual Conference

he was pretty proud to solve that urogenital schistosomiasis on colleague Colin Sutherland, based at the London School of the island was actually an imported infection and not naturally Hygiene and Tropical Medicine. Working with Colin, the SIMI endemic see http://www.sciencedirect.com/science/article/pii/ projected revealed many new dimensions in the co-epidemiology S0001706X12003117. of schistosomiasis and malaria co-infection in young children. The SIMI team helped to pioneer how treatment could be delivered in Russ completed his PhD in 1995 at the Natural History Museum, future assessing horizontal and vertical delivery of public health London which is also WHO Reference Centre for schistosomiasis. services. His studies were undertaken mainly on Zanzibar where he was able to show that urogenital schistosomiasis has a clearly demarked This helped to break down siloes not only in the diseases but also endemic and non-endemic zone on the island. Taking advantage of in the infrastructures that arise around them for their control. Russ his transmission maps, there have been major efforts to eliminate mentioned “Any young child deserves to be healthy whatever the the disease there spearhead by ZEST: Zanzibar Elimination of disease doesn’t (s)he?”. Schistosomiasis Transmission (see http://score.uga.edu/projects/ elimination-of-schistosomiasis/). Looking to the future and sustainable development goals, control of NTDs are framed within it. Thus control methods have to expand Russ therefore feels a close affinity with Leiper in that he was also their thinking beyond medicine and think of how agriculture, water looking for clues to find out about the disease in order to work and socio-economic development are all interconnected. This is out an intervention. To see the bigger picture of this disease Russ part of the ethos of COUNTDOWN, to develop multidisciplinary used a combination of molecular genetics (for snail identification) teams conducting transdisciplinary research. Directing this team and spatial epidemiology (for GPS mapping) as well as having an Russ remarked he was fortunate to employ several talented staff. understanding of the ecology of the parasites in people (water contact behavior) to look at the whole lifecycle and how all of the ASP member and recent PhD graduate from ANU, Suzy Campbell, components fitted together on the island. joined the project at the Liverpool School of Tropical Medicine as an epidemiologist to review the control strategy for scihstosomiasis In 2002 Russell was employed to work as a field coordinator with and STH, a change from the cross-sectoral approach. Addressing Alan Fenwick on a Bill & Melinda Gates funded Schistosomiasis the epidemiology of of these diseases the team believe that it Control Initiative, primarily concerned with distributing might not be “neglected populations” but rather the inequality of praziquantel medications to children. He was in charge of treatment causing issues with the delivery of treatments. To make developing the field programme to measure indicators of how a difference to these vulnerable communities they will provide this treatment was changing the morbidity of the disease. As a opportunities for communities to access alternative interventions result, National Control Programmes were created in Tanzania, against these disease and explore new treatment strategies. Uganda, Zambia, Niger, Mali and Burkina Faso. From that point on Russell was interested in public health interventions and the health Both Suzy and Russ know just how important this research is system in Africa, and made many trips to each country. Having and the feasibility of changing strategies to treat schistosomiasis visited Uganda (like Leiper) many times Russ saw the important and STHs in Ghana and Cameroon is filled with challenges: treatment gap in young children (preschool-aged) that needed cost efficiency and bottlenecks mean that this research is not solving with targeted epidemiological research. This lead to SIMI: just a straightforward biological investigation. Implementing schistosomiasis in mothers and infants project as funded by the the research needs to be built into the health systems so that Wellcome Trust. an integrated program of research combining epidemiology, parasitology and social science takes place. This integrated view The SIMI project was also in collaboration with an Australian will make a difference to people.

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Prizes for the best poster and oral Best Early Career Researcher Presentation: Runner Up, Student Poster: Dhanasekaran presentations Adele Lehane, ANU. Sakhivel, Monash University.

Best Student Poster: Mohammed Runner Up, Student Presentation: Runner Up, ECR Presentation: Samantha Al-Hasnawy, Monash University. Erick Tjhin, ANU Emery, WEHI

Best Student Presentation: Deepani Winner, ICTMM Best Poster Presentation: Fernando, QIMR Berghofer Michelle Stanton

Page 36 Journals

consideration. If the submitted image is selected for the journal cover, it, and the associated article, will be promoted on Facebook, Twitter and Instagram.

• Together with Dale Seaton of Elsevier, Brian developed a talk, tailored to early-career researchers, based around the Elsevier Publishing Campus on ‘how to publish your papers’. Brian and Dale first presented 2016 HIGHLIGHTS INTERNATIONAL the talk at a very successful ERC breakfast at the joint NZSP+ASP conference in Auckland, then again at JOURNAL FOR PARASITOLOGY (IJP) MAM2016 in Lorne. Brian has developed a modified version of the talk and has delivered this over the last 2 www.journals.elsevier.com/international-journal-for- years to the students on the ASP parasitology course in parasitology Kiola. Let Brian know if you would like him to give this presentation to your institution. Editor In Chief: Brian Cooke • Nick Clark and colleagues (45:14 pp 891-899) created The major IJP news in 2016 was that the journal broke the the most social media activity to date for an IJP paper ‘4 barrier’ with an Impact Factor of 4.242 (© Thomson and was featured on ABC News, Radio National and the Reuters Journal Citation Reports 2016). The IJP team remain BBC world Service. His paper is a fascinating piece of confident that the IJP will continue to prosper under Brian’s work on the high prevalence of avian malaria parasites leadership and are looking forward to what lies ahead. in invasive Indian Mynahs and their potential threat to native wildlife and birds in Australia and elsewhere. Some other highlights from 2016 included: At the close of 2016, the editors were working on some • IJP continued the experiment with social media, with Special Issues for 2017: Molecular Approaches to Malaria a Facebook page started in March 2015 (‘liked’ by (MAM) 2016 conference (publication planned in February) 1480 people at close of 2016; www.facebook/IJPara) and Singapore Malaria Network (SingMalNet) 2016 meeting and Twitter in October 2015 (83 followers; @IJPara (publication planned for March) and now also on Instagram (45 followers; ijpara). Look for the green on black IJP logo (the ‘real’ IJP page). A The editors are grateful to the Editorial Board members, ‘story behind the cover’ is featured for each IJP issue, reviewers and authors who continue to make IJP the highest so those who have a paper accepted for publication are cited journal dedicated to parasitology and publishing encouraged to create a cover image and submit it for original research articles.

The twelve IJP covers for 2016

Page 37 Journals

valuable alternative to the Impact Factor – more information INTERNATIONAL can be found on the IJP:PAW website by following the JOURNAL FOR PARASITOLOGY links. IJP:PAW's 2015 CiteScore is 2.7, ranking us 12 out of 58 parasitology journals, and ahead of Acta Tropica, Parasitology, Veterinary Parasitology and Experimental Parasitology.

Selected papers from 2016 2016 HIGHLIGHTS INTERNATIONAL JOURNAL FOR PARASITOLOGY: Hillman A, Ash A, Elliot A, Lymbery A, Perez C, Thompson RCA. Confirmation of a unique species of Giardia, parasitic PARASITES AND WILDLIFE (IJP:PAW) in the quenda (Isoodon obesulus). Int J Parasitol Parasites www.journals.elsevier.com/international-journal-for- Wildl. 2016 Jan 12;5(1):110-115 parasitology Yang R, Brice B, Ryan U. Morphological and molecular Editors: R.C. Andrew Thompson, Lydden Polley characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot As 2016 drew to a close, submissions to IJP:PAW continued (Purpureicephalus spurius, Kuhl, 1820) in Western to arrive at a healthy rate and had topped the 80 mark Australia. Int J Parasitol Parasites Wildl. 2016 Jan 12;5(1):34- by the end or December – a significant increase over the 9 previous couple of years.The quality of articles continued to be very high and the fantastic diversity of topics covered is Chilton NB, Huby-Chilton F, Koehler AV, Gasser RB, a major strength of the journal. Subjects ranged from host- Beveridge I. Detection of cryptic species of Rugopharynx parasite co-infections, (Nematoda: Strongylida) to climate change, from the stomachs of ectoparasites on deep Australian macropodid sea fish, and parasites marsupials. Int J Parasitol of bees. A special issue Parasites Wildl. 2016 Apr on Invasions underway 19;5(2):124-33 and two more special issues are planned in Hobbs RP, Elliot AD. 2017. A new species of Potoroxyuris (Nematoda: Although the journal Oxyuridae) from the was waiting for woylie Bettongia Thomson’s to release its penicillata (Marsupialia: Impact Factor, Elsevier Potoroidae) from have introduced their southwestern Australia. metric, CiteScore, Int J Parasitol Parasites which is likely to receive Wildl. 2016 Jun broad acceptance as a 27;5(3):211-6

Image : the woylie (Bettongia penicillata) in the SW of Western Australia (Murdoch Parasitology). See Hobbs and Elliot, A new species of Potoroxyuris

Page 38 Journals

Raza A, Kopp SR, Bagnall NH, Jabbar A, Kotze AC. Effects

INTERNATIONAL of in vitro exposure to ivermectin and levamisole on the JOURNAL FOR expression patterns of ABC transporters in Haemonchus PARASITOLOGY contortus larvae Int J Parasitol Drugs Drug Resist. 2016 Aug;6(2):103-15

Bourguinat C, Che H, Mani T, Keller K, Prichard RK. ABC-B transporter genes in Dirofilaria immitis. Int J Parasitol Drugs Drug Resist. 2016 Aug;6(2):116-24 2016 HIGHLIGHTS INTERNATIONAL JOURNAL FOR PARASITOLOGY: DRUGS Leitsch D, Müller J, Müller N. Evaluation of Giardia lamblia AND DRUG RESISTANCE (IJP:DDR) thioredoxin reductase as drug activating enzyme and as drug target. Int J Parasitol Drugs Drug Resist. 2016 www.journals.elsevier.com/international-journal-for- Dec;6(3):148-153. parasitology-drugs-and-drug-resistance/ Kumarasingha R, Karpe AV, Preston S, Yeo TC, Lim DS, Editors In Chief: Andrew Kotze & Kevin Saliba Tu CL, Luu J, Simpson KJ, Shaw JM, Gasser RB, Beale DJ, Morrison PD, Palombo EA, Boag PR. Metabolic profiling IJP:DDR continues to grow, with submissions increasing and in vitro assessment of anthelmintic fractions of Picria each year. fel-terrae Lour. Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):171-178 Selected papers from 2016 Tran PN, Tate CJ, Ridgway MC, Saliba KJ, Kirk K, Maier AG. Mani T, Bourguinat C, Keller K, Human dihydrofolate reductase Carreton E, Peregrine A, Prichard influences the sensitivity of the RK. Polymorphism in ion channel malaria parasite Plasmodium genes of Dirofilaria immitis: falciparum to ketotifen - A cautionary Relevant knowledge for future tale in screening transgenic parasites. anthelmintic drug design Int J Int J Parasitol Drugs Drug Resist. 2016 Parasitol Drugs Drug Resist. 2016 Dec;6(3):179-183 Dec;6(3):343-355. Zhang Z, Gasser RB, Yang X, Yin F, Preston S, Jiao Y, Jabbar A, McGee Zhao G, Bao M, Pan B, Huang W, SL, Laleu B, Willis P, Wells TN, Wang C, Zou F, Zhou Y, Zhao J, Fang R, Gasser RB.Screening of the Hu M. Two benzimidazole resistance- 'Pathogen Box' identifies an associated SNPs in the isotype-1 approved pesticide with major β-tubulin gene predominate in anthelmintic activity against Haemonchus contortus populations the barber's pole worm Int J from eight regions in China. Int J Parasitol Drugs Drug Resist. 2016 Parasitol Drugs Drug Resist. 2016 Dec;6(3):329-334 Dec;6(3):199-206 Image from Mani et al Polymorphism in ion channel genes of Dirofilaria immitis

Page 39 Public Engagement and Outreach

IN 2016, THE OUTSTANDING Discovery, Griffith University, held a stall over two days at the Royal ENTHUSIASM OF ASP MEMBERS FOR Brisbane Show. PUBLIC ENGAGEMENT AND OUTREACH Jessica Johnson-Mackinnon and collaegues held an event for RESULTED IN NUMEROUS HIGHLIGHTS children between 5 and 10 year old at the University of Tasmania's AND NOVEL INITIATIVES AND EVENTS. Institute for Marine and Antarctic Studies Open Day. (pictured below) In the folliwng pages we highlight the Murdoch Parasitology team in Perth, National Science Week in Cairns, outreach at GTAC Rebecca Traub (The University of Melbourne): Magnificent in Melbourne, Science Meets Parliament in Canberra and the Microscopy – Life under a Lens. Gene Technology Access Centre, Parasites: Life Undercover exhibition in Brisbane. 21st August 2016. Middle and high-school students.

Other outreach activities reported by ASP mambers Rebecca Traub (The University of Melbourne): Year 10 work experience, 24th -29th November 2016, Gene Technology Access On the 26th April the Griffith University Eskitis Institute for Drug Centre. Supervised four students conducted a research project to Discovery and the Australian Society for Parasitolgoy co-sponsored screen for novel filarial in canine hippoboscid flies from Rajasthan, a public outreach event around World Malaria Day 2016. This India. event focused on two activities – a series of short talks to promote awareness of malaria and a primary school outreach component Rebecca Traub (The University of Melbourne) was Invited speaker to promote malaria awareness through distribution of “What is at “One Health Day”, Department of Health, Victorian Government, malaria?” activity packs. Melbourne, 3rd November 2016.

Amy Jones and other members of the Eskitis Institute for Drug

Page 40 Public Engagement and Outreach

ASP MEMBER OUTREACH: MURDOCH diseases to native species.

Outreach activities will be a major feature of the project, with ANDY THOMPSON AND ALAN LYMBERY community workshops held in the urban area. The first of these HAVE PARTNERED WITH THE EASTERN was held a few weeks ago with presentations from Alan, Alison METROPOLITAN REGIONAL COUNCIL TO Hillman and Andy. It was well attended and there was considerable interest from the public about which native animals are at risk RAISE AWARENESS OF ‘ONE HEALTH’ AS from human activities, what parasitic diseases humans can IT APPLIES TO URBAN WILDLIFE. inadvertently give to wildlife, and the threat of invasive species. Supported by a LotteryWest grant of almost $200,000. the THE PARASITOLOGY GROUP AT ‘Healthy Wildlife Healthy Lives’ project aims to educate the community about ‘One Health’ focusing on human and domestic MURDOCH PRESENT A HANDS-ON animal’s contact with wildlife in urban areas. The hope is that WORKSHOP TO 43 YEAR 12 STUDENTS. this will help the community to interact positively with wildlife and protect and conserve the environment for the benefit of The Parasitology Group hosted ‘A day in the life of a Murdoch both wildlife and people. The project will initially focus on Perth’s University student’, with Parasitology representing the Biomedical Eastern Region and will involve the community in the creation of Sciences at this event. The session was the most popular of all an innovative, low cost and sustainable those offered at the university, hosting model of community education, 43 year 12 students from a range of high including a dedicated website. The schools for a 1 hour hands-on workshop on project will work closely with voluntary parasitology. A ten minute talk was given by wildlife organisations as well as the Dr Amanda Ash introducing the students to Department of Parks and Wildlife and parasites and their importance. The students local veterinarians. Once developed, the then worked through a series of microscopes project is expected to be applied beyond in the laboratory, with a range of real parasite Perth’s Eastern Region. specimens to examine, including a flea identification station, whole specimens of Many One Health projects in Australia gut and heart tissue containing worms, and have focused on the transfer of diseases microscope slides of malaria, trypanosomes from animals to humans and the public health consequences and histology sections of flukes and cysts. rather than the transfer of diseases from humans to animals and the consequences for wildlife health and conservation. This new The students seemed engaged for the hour-long workshop, and and innovative project will seek to address this gap. The project many were suitably grossed out and fascinated by the specimens on offer. will be concerned with a number of parasites such as Toxoplasma and Giardia and how human activities can transmit infections to wildlife such as backyard feeding. The project will also educate the community about the important impact humans have on the environment and native animals, for example, releasing invasive fish species like goldfish into waterways that then not only Image: Alison Hillman at an outreach event compete with native species of fish, but also transfer new parasitic for Healthy Wildlife Healthy Lives

Page 41 Public Engagement and Outreach

ASP MEMBER OUTREACH: NATIONAL SCIENCE WEEK into the science of our minds at Café Scientifique. Professor Nimmo also discussed his groundbreaking research on a drug to stop the brain swelling after a head injury Dr Kate Miller, ASP LISA JONES WAS AWARDED A member from James Cook University gave a wonderful talk on COMMONWEALTH OF AUSTRALIA toxoplasma describing the parasite as a "puppetmaster”. Dr NATIONAL SCIENCE WEEK GRANT Tasmin Rymer and Dr Ernest Jennings gave talks about a person's perception of pain and bizarre animal behaviour. FOR TWO EVENTS AT THE TANKS ARTS CENTRE IN CAIRNS. This National Science Week event also featured Brainwave, an interactive art piece created by JCU Creative Media Unit staff and students, that When hip-hop singer uses visual arts Naomi Wenitong woke up from a coma and multimedia to three months after connect the science being pronounced of our minds with dead in a car accident the research taking it was her bushy place around us. eyebrows that shocked her, not the PechaKucha Night multiple fractures featured ASP in her face or her members Denise complete loss of memory. Doolan and Alex Loukas who gave wonderful 6 minute “My eyebrows were presentations a mess, but my nails about worms and were amazing so I vaccines along with had to get on the four other James phone to my sister Cook University and ask her what she researchers had been thinking,” presenting on bees, Naomi laughed. solar energy and climate change.

The accident and Naomi’s incredible recovery from a brain injury made national headlines as the Indigenous performer had shot to fame as part of the ARIA chart-topping duo Shakaya in 2002.

Naomi relived her often hilarious story and share her music through this National Science Week event that tackles the serious Top left: young parasitologists enjoying parasite craft table. Top right: Kate Miller "Parasite Puppetmaster". Bottom issue of brain injury through science, art and music . Professor Alan left: Alan Nimmo and Naomi Wenitong. Bottom right: the Nimmo from James Cook University interviewed Naomi, delving crowd enjoying Cafe Scientfique.

Page 42 Public Engagement and Outreach

TWO STORIES FROM GTAC about ticks. I learnt about genetics, bioinformatics, morphology, chemistry, taxonomy and parasites. I also met some really nice, like-minded people who all had a passion for science.” (SK, Year 10, DURING MAY AND JUNE 2016, Wangaratta High School). EIGHT YEAR 10 STUDENTS FROM SIX VICTORIAN COUNTRY SCHOOLS UNDERTOOK PARASITOLOGY-THEMED IN ITS SEVENTH YEAR, THE PARASITES RESEARCH PROJECTS FOR THEIR WORK IN FOCUS STUDENT PROGRAM IS A EXPERIENCE PLACEMENTS AT GTAC, SPECIAL PROGRAM DEVELOPED AND THE GENE ACCESS TECHNOLOGY RUN AS A COLLABORATION BETWEEN CENTRE IN MELBOURNE. THE GENE TECHNOLOGY ACCESS CENTRE (GTAC) AND THE ASP. REPORT During May and June 2016, eight Year 10 students from six BY DR TONY CHIOVITTI, GTAC Victorian country schools undertook parasitology-themed research This program aims to raise awareness projects for their work experience of the societal and economic placements at GTAC. The projects consequences associated with enabled the students to immerse parasitism through impacts on themselves for one week working in ecosystem dynamics, agricultural an authentic research environment. security, and human health. The The students collaborated with program also exposes secondary practising parasitologist, Mackenzie school students to a diverse range Kwak (AgriBio, LaTrobe University) of careers that are supported by and educator, Tony Chiovitti (GTAC) training in parasitology. In the exploring the phylogenetics of Victorian school curriculum, the parasitic ticks of birds and platypuses. only explicit mention of parasitism For their analyses, students used is in Unit 1 of the VCE Biology study scanning electron and optical microscopy to collect morphological design under topics on relationships between organisms within data, as well as generating DNA barcode sequences from their an ecosystem. Technologies for studying parasites as pathogens specimens. During the placements, Dr Abdul Jabbar, a Senior address a number of key skills and techniques relevant to key Lecturer, took the students on a tour of the parasitology labs at content addressed in the broader VCE Biology study design. For the Faculty of Veterinary and Agricultural Science, University of these reasons, the Parasites in Focus student program is designed Melbourne. The tour gave students insights into the burden of primarily for students of Years 10 and 11. parasitic disease in companion animals and Australian livestock.

The students provided glowing feedback of their experience at the Opening address by Robin Gasser conclusion of the placements. For example, one student wrote: “I learnt so much but even though we focused on ticks, it wasn’t all For Parasites in Focus, Robin Gasser introduced students to the

Page 43 Public Engagement and Outreach

main groups of parasites, their complex life cycles, and some of the other species. Working in the GTAC computer laboratory, students afflictions they cause in their hosts. During his presentation, Robin used the computer simulation software, NetLogo, to investigate circulated samples of the parasites among the audience. At the the effects of various natural enemies on populations of the light conclusion of the presentation, students asked probing questions brown apple moth, a pest of the grape industry. Evaluation of their ranging from mechanisms that adapt parasites to their way of life, mathematical models led students to conclude that the parasitoid to details of infection processes and their treatment. wasp, Trichogramma was the most suitable biological control.

Hooked on Parasites The students loved the hands-on experiments and workshops:

Parasitic diseases cost the Australian sheep industry almost “This workshop was very interesting and fun, seeing how this kind $900 million per annum. Working with scientist mentors in the of lab works was a great experience” GTAC laboratories, students applied techniques of the veterinary pathologist to diagnosing a parasitic infection of sheep. An “It was good to see how some scientists work in controlling pest assessment of clinical signs, faecal egg counts, and examination populations, and how modern technologies integrate into the of larvae and adult worms led students to deduce the sheep were (real) world” infected by the nematode, Haemonchus contortus. Students concluded by proposing strategies to This special event program could manage the health of the flock. not have been delivered to the standard GTAC had envisaged A Case of Cross-Border without the generous support of a Detection grant for $2,000 from the Victorian branch of the ASP. Malaria represents the world’s biggest health issue caused by parasites. Working in the GTAC laboratories with scientist mentors, students used biotechnology to identify the specific malarial parasite infecting a tourist who had returned from overseas. Applying polymerase chain reaction and gel electrophoresis experiments, students determined the tourist was infected by the deadliest type, Plasmodium falciparum. The result is crucial for informing the patient’s treatment.

Parasites Getting it Under Control

Australia is a world leader in the field of biological control, in which pathogens, predators, or parasites are harnessed to specifically control populations of the target pest with minimal disruption to

Page 44 Public Engagement and Outreach

SCIENCE MEETS PARLIAMENT 2016 Network Ten and Alison Carabine Political Editor ABS RN Breakfast. There was some discussion about translating science into policy PROFESSOR BARBARA NOWAK OF and what it requires. THE UNIVERSITY OF TASMANIA AND That afternoon was used to prepare scientists for the meetings DR SHOKOOFEH with parliamentarians. This was achieved by presentations from former participants and SHAMSI OF rehearsing organised by Dr Rod CHARLES STURT Lamberts ANU and Dr Will Grant UNIVERSITY ANU. REPRESENTED In the evening we attended THE ASP AT Gala Dinner in the Great Hall of Parliament House. Bernie SCIENCE MEETS Hobbs ABC science broadcaster PARLIAMENT 2016 was the MC, presentations were given by a number of IN CANBERRA. guest speakers including The THIS REPORT BY Hon Christopher Pyne MP, BARBARA NOWAK. Minister for Industry, Innovation and Science and The Hon Bill Shorten MP, Leader of the Science meets Parliament was Opposition. organised on 1 and 2 March by Science and Technology I shared my table with The Australia. This is an annual event Hon Alannah MacTiernan MP with the first day run in the Hotel member for Perth (ALP) who was Realm and the second in the very interested in aquaculture Parliament House. and made a real effort to talk to all scientists around the table. On the first day after welcome Senator Janet Rice (VIC, AG) was and overview, Prof Brian Schmidt also sitting at our table. AC, ANU gave the opening address. There were a couple of Next morning we joined a long presentations from the sponsors, queue to Parliament House. I including CropLife and ANSTO. (together with Dr Sophie Lewis Ms Sue Weston, Deputy ANU, Dr Danielle Martin ANSTO Secretary DIIS talked about National Innovation and Science and Dr Maxine Roberts ANSTO) met with The Hon Julie Collins Agenda. There was a lot of information provided by journalists. MP, member for Franklin (ALP), who was concerned about current In Meet the Media the chairKylie Walker AAS discussed turning cuts at CSIRO and was very interested in equal opportunities science into news with Paul Bongiorno AM, contributing editor to including gender equity and supporting more women going into

Page 45 Public Engagement and Outreach

science. I have talked about outreach activities sponsored by ASP I learnt a lot and was inspired by some of the presentations, in and their role in encouraging young people to study science. The particular Prof Brian Schmidt and Dr Alan Winkel gave excellent timing of my meeting with parliamentarian meant that I missed talks. It was also good to get a general feel for political climate for presentations from Prof science and the increasing Ian Chubb and Senator importance of collaboration Kim Carr. with industry and research innovation. There was a After my meeting it was lot of great advice helpful time for lunch at National not only in contacts with Press Club. The National parliamentarians. I really Press Club address was enjoyed the dinner and delivered by Dr Alan Leader of the Opposition’s Finkel AO, Chief Scientist address. There were for Australia. amazing opportunities for networking and I met Following lunch we people I have not seen returned to Parliament since I was doing my PhD. I House to attend Question would like to thank the ASP Time. Last session of the for sending me to Science day was a Parliamentary meets Parliament 2016. Forum (Science and politics, how do they mix) I encourage more Early with Prof Aidan Byrne Career Researchers who CEO ARC, The Hon Karen are members of the ASP to Andrews MP, The Hon participate in the future. Richard Marles MP and I thought it was really The Hon Adam Bandt MP beneficial to send an ECR chaired by Genevieve and a more experienced Jacobs and followed by researcher. drinks and closure of the event.

The Hon Alannah MacTiernan MP found me Previous page: Professor during the drinks to give Barbara Nowak and Dr me a copy of Scaling Up – Shokoofeh Shamsi at Science meets Parliament Inquiry into Opportunities 2016 in Canberra. This page for Expanding top: Shokoofeh with Hon Aquaculture in Northern Christopher Pyne Minister for Industry, Innovation and Australia prepared by Joint Select Committee on Northern Australia Science. Bottom: Shokoofeh and Barbara with Hon which she is Deputy Chair. Bill Shorten MP, Leader of the Opposition.

Page 46 Public Engagement and Outreach

PARASITES: LIFE UNDERCOVER “Parasites are our world at the Australian Society of Parasitology and we are truly excited to partner with Queensland Museum to bring this unique exhibition to Brisbane, which will coincide with A NUMBER OF ASP PARASITOLOGISTS, our annual conference,” Professor Emery said. INCLUDING TOM CRIBB, GLEN COLEMAN AND VICKY AVERY, “We are always looking a ways to connect with the community and recently have had artists collaborate with us on a unique project GAVE FREE TALKS AT THE QUEENSLAND exploring the theme of parasites and health. One of the signature MUSEUM IN AUGUST 2016 TO HELP artworks of this project, Gula Guri mayin, which means heal the body by Cairns artist Bernard Lee Singleton will feature in the CELEBRATE NATIONAL SCIENCE WEEK exhibition.” AND PROMOTE THE EXHIBITION PARASITES LIFE UNDERCOVER. Parasites: Life Undercover was developed by the Museum of Natural History Berlin Germany with support of Bayer HealthCare Animal Health. Queensland Museum CEO and Director, Professor Suzanne Miller said the exhibition wouldl give visitors an opportunity to Parasites: Life Undercover was supported by the Australian understand the impacts they have on both animals and humans. Society of Parasitology and ran from 10 August 2016 – 27 January 2017 on Level 2, Queensland Museum. “Parasites: Life Undercover highlights the significance parasites have on our daily lives, from the common pests such as fleas and lice such as treating heartworm in a pet to the more serious epidemics and threats such as malaria and those skin and gut parasites costing our livestock industries over a billion dollars each year,” she said.

“Parasitism is a highly successful way of life. Parasites are a real and serious threat to all living things and this exhibition will showcase the fascinating evolution of parasites, their resilient life cycles and their ability to move from one host to another in order to not only survive, but in many cases, thrive.”

“Having plagued us for centuries, parasites have certainly gained a bad reputation, but you will also see the positive role parasites can play in the medical industry and in developing our immune systems.”

Parasites: Life Undercover was supposted by the Australian Society of Parasitology. ASP president Professor David Emery said the Society hoped it would promote the knowledge and fascination of parasitology to the general public.

Page 47 Public Engagement and Outreach

Images from the exhibition Parasites: Life Undercover.

Page 48 Education

A W

N S U N

K , I S O U L P O M A two-week parasitology course for A A COASTAL C postgraduates and Early Career Researchers

THE 2016 ASP ADVANCED COURSE, CONCEPTS IN PARASITOLOGY, TOOK PLACE BETWEEN NOVEMBER 27TH AND DECEMBER 10TH AT THE ANU’S BEAUTIFUL KIOLOA CAMPUS, A FIELD STATION ON THE NSW SOUTH COAST.

Course Convenor was Associate Professor Alex Maier (ANU). A large faculty of prominent parasitologists contributed during the two weeks.

• Rob Adlard (Queensland Museum) • Steve Lee (Australian National University) • Glenn Andersen (Virbac Australia) • Alan Lymbery (Murdoch University) • Nigel Beebe (University of Queensland) • Alexander Maier (Australian National University) • Ian Beveridge (University of Melbourne) • Geoff McFadden (University of Melbourne) • Susan de Burgh (Bayer Australia) • Nicki O'Donoghue (University of Queensland) • Brian Cooke (Monash University) • Peter O’Donoghue (University of Queensland) • Tom Cribb (University of Queensland) • Ryan O'Handley (University of Adelaide) • Christian Doerig (Monash University) • Stuart Ralph (University of Melbourne) • Giel van Dooren (Australian National University) • Louise Randall (University of Melbourne) • David Emery (University of Sydney) • Melanie Rug (Australian National University) • Robin Gasser (University of Melbourne) • Una Ryan (Murdoch University) • Paul Giacomin (James Cook University) • Kevin Saliba (Australian National University) • Stephanie Godfrey (Murdoch University) • Robyn Slattery (Monash University) • David Jenkins (Charles Sturt University) • Brad Sleebs (Walter and Eliza Hall Institute) • Aaron Jex (University of Melbourne/WEHI) • Christina Spry (Australian National University) • Amy Jones (Griffith University) • Melissa Sykes (Griffith University)

Page 49 Education

After the welcome address, Dr. Steve Lee (ANU) kicked- For the 2016 course we received 23 applications, which off the event with a lecture on mobile microscopes and allowed us to fill the 16 available places. Gender distribution a workshop to manufacture lenses to convert mobile of participants was 15 females and 1 male (also reflective of phones into microscopes. The workshop was followed the applications). by a Welcome BBQ. During the first two days three ANU parasitology labs (K. Saliba, M. Rug, and G. van Dooren) 14 participants were PhD students, 1 was employed in the opened their doors to the course participants sharing public service, and 1 worked in industry. We had 2 students the expertise in physiology, imaging and cell biology and from the USA (Louisiana State University). Two students taking advantage of the instrumentation that is available at were charged the ASP member rate (to accommodate for the ANU Canberra campus. Erick Tjhin, Esther Rajendran, financial hardship created by the floods in Baton Rouge in Kathryn Parker, Sherry He, Joanne Lee, Daryl Webb and August 2016), but both joined the ASP. Five of the fourteen Frank Brink enthusiastically supported the modules run at PhD students studying at an Australian University were the ANU campus in Canberra. international students.

In Kioloa, 27 lecturers filled the course with a wealth of As in previous years, the background of the participants content. The concepts were mainly based on the themes was very diverse adding to the vibe of the course (and from previous years, representing important and current representing the ASP constituency). There was plenty of topics in Parasitology. 7 lecturers were the first time in opportunity to exchange perspectives and experiences and Kioloa, 6 lecturers also participated last year and 14 have this was appreciated by the participants. done all three years. The following committee members were responsible for In a spirit of continuous improvement, the 2016 course was creating the framework for the ASP course: Una Ryan, Stuart refined in line with suggestions made in the previous years. Ralph, Arraon Jex, Rob Adlard and Alex Maier.

Above: course participants at Concepts in Parasitology 2016

Page 50 Statistical Snapshot 2016

Number of (active) members; Websites

Australian Society for Parasitology Incorporated (ASP) had 475 ASP web site | www.parasite.org.au financial members in 2016. ASP Facebook page | www.facebook.com/ASParasitology ASP Twitter account | twitter.com/AS_Para Number of ECRs funded to do various activities; ASP YouTube channel | www.youtube.com/user/ ASPParasiteNetwork 108 students were given funding assistance to attend the 2016 ASP The ASP’s Google Plus account | https://plus.google.com/100 Annual Conference/ICTMM 2016. 938254649203422506#100938254649203422506/posts

10 students and ECRs received JD Smyth and ASP Network Researcher International Journal for Parasitology web site | www.journals. Exchange, Training and Travel, out of a total of 12 awards made in elsevier.com/international-journal-for-parasitology 2016. IJP Facebook: www.facebook.com/IJPara IJP Twitter: twitter.com/IJPara Conferences supported; IJP Instagram: www.instagram.com/ijpara/

2016 ASP Annual Conference as part of ICTMM 2016 which was International Journal for Parasitology – Parasites and Wildlife attended by over 1500 delegates. | www.journals.elsevier.com/international-journal-for- parasitology-parasites-and-wildlife/ Number of publications produced; IJP:PAW Facebook | www.facebook.com/IJPPAW/

Over 550 printed publications. International Journal for Parasitology – Drugs and Drug Resistance website | www.journals.elsevier.com/international- Research funding received; journal-for-parasitology-drugs-and-drug-resistance/ IJP:DDR Facebook | www.facebook.com/IJPDDR/ In 2016, Australia’s parasitologists received over 40 research grants securing more than $33 million in new research grant funding.

Newsletters

Three newsletters were published during the year, pictured right.

Volume 27.1 May 2016 Volume 27.2 September 2016 Volume 27.3 November 2016

Page 51 Appendix 1: Publications by ASP Members in 2016

WHERE APPLICABLE, LINKS TO Pathog. 2016 Aug 4;12(8):e1005765. doi: 10.1371/journal. ABSTRACTS IN PUBMED HAVE BEEN ppat.1005765. eCollection 2016 Aug PROVIDED FOR 2016 PUBLICATIONS. Ansari HR, Templeton TJ, Subudhi AK, Ramaprasad A, Tang J, Lu F, Naeem R, Hashish Y, Oguike MC, Benavente ED, Clark TG, Sutherland CJ, Barnwell JW, Culleton R, Cao J, Pain A. Genome- Health and Wellbeing scale comparison of expanded gene families in Plasmodium ovale wallikeri and Plasmodium ovale curtisi with Plasmodium malariae and with other Plasmodium species. Int J Parasitol. 2016 Adekunle AI, Cromer D, Davenport MP. Artemisinin-Based Oct;46(11):685-96. doi: 10.1016/j.ijpara.2016.05.009. Epub 2016 Treatments in Pregnant Women with Malaria. N Engl J Med. 2016 Jul 5. Jul 21;375(3):283. doi: 10.1056/NEJMc1604709#SA1 Antony HA, Das S, Parija SC, Padhi S. Sequence analysis of pfcrt Aghazadeh M, Harvie MC, Owen HC, Veríssimo C, Aland KV, and pfmdr1 genes and its association with chloroquine resistance Reid SA, Traub RJ, McManus DP, McCathy JS, Jones MK. in Southeast Indian Plasmodium falciparum isolates. Genom Comparative pathogenesis of eosinophilic meningitis caused by Data. 2016 Apr 18;8:85-90. doi: 10.1016/j.gdata.2016.04.010. Angiostrongylus mackerrasae and Angiostrongylus cantonensis in eCollection 2016 Jun. murine and guinea pig models of human infection. Parasitology. 2016 Sep;143(10):1243-51. doi: 10.1017/S003118201600069X. Antony HA, Parija SC. Antimalarial drug resistance: An overview. Epub 2016 Jun 9. Trop Parasitol. 2016 Jan-Jun;6(1):30-41. doi: 10.4103/2229- 5070.175081. Review. Agomo CO, Oyibo WA, Sutherland C, Hallet R, Oguike M. Assessment of Markers of Antimalarial Drug Resistance in Antony HA, Pathak V, Ghosh K, Parija SC. Comparison Plasmodium falciparum Isolates from Pregnant Women in Lagos, of protein expression pattern between the Plasmodium Nigeria. PLoS One. 2016 Jan 25;11(1):e0146908. doi: 10.1371/ falciparum chloroquine-resistant RKL9 and chloroquine-sensitive journal.pone.0146908. eCollection 2016. MRC2 strains. Trop Parasitol. 2016 Jul-Dec;6(2):136-140.

Alvarez Rojas CA, Fredes F, Torres M, Acosta-Jamett G, Alvarez Antony HA, Pathak V, Parija SC, Ghosh K, Bhattacherjee A. JF, Pavletic C, Paredes R, Cortés S; Ministry of Health, SAG and Whole transcriptome expression analysis and comparison of two other organizations attending the meeting. First meeting “Cystic different strains of Plasmodium falciparum using RNA-Seq. Genom echinococcosis in Chile, update in alternatives for control and Data. 2016 Apr 23;8:110-2. doi: 10.1016/j.gdata.2016.04.004. diagnostics in animals and humans”. Parasit Vectors. 2016 Sep eCollection 2016 Jun. 13;9:502. doi: 10.1186/s13071-016-1792-y. Erratum in: Parasit Vectors. 2016 Nov 15;9(1):583. Antony HA, Pathak V, Parija SC, Ghosh K, Bhattacherjee A. Transcriptomic Analysis of Chloroquine-Sensitive and Chloroquine- Amiar S, MacRae JI, Callahan DL, Dubois D, van Dooren GG, Resistant Strains of Plasmodium falciparum: Toward Malaria Shears MJ, Cesbron-Delauw MF, Maréchal E, McConville MJ, Diagnostics and Therapeutics for Global Health. OMICS. 2016 McFadden GI, Yamaryo-Botté Y, Botté CY. Apicoplast-Localized Jul;20(7):424-32. doi: 10.1089/omi.2016.0058. Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Apte SH, Stephenson RJ, Simerska P, Groves PL, Aljohani S, Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase. PLoS Eskandari S, Toth I, Doolan DL. Systematic evaluation of self-

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adjuvanting lipopeptide nano-vaccine platforms for the induction O, Kwiatkowski DP, Nosten F, Price RN. Genomic Analysis Reveals of potent CD8(+) T-cell responses. Nanomedicine (Lond). 2016 a Common Breakpoint in Amplifications of the Plasmodium vivax Jan;11(2):137-52. doi: 10.2217/nnm.15.184. Epub 2015 Dec 11. Multidrug Resistance 1 Locus in Thailand. J Infect Dis. 2016 Oct 15;214(8):1235-42. doi: 10.1093/infdis/jiw323. Epub 2016 Jul 24 Armua-Fernandez MT, Joekel D, Schweiger A, Eichenberger RM, Matsumoto J, Deplazes P. Successful intestinal Echinococcus Bachmann A, Petter M, Krumkamp R, Esen M, Held J, Scholz multilocularis oncosphere invasion and subsequent hepatic JA, Li T, Sim BK, Hoffman SL, Kremsner PG, Mordmüller B,Duffy metacestode establishment in resistant RccHan™:WIST rats MF, Tannich E. Mosquito Passage Dramatically Changes var after pharmacological immunosuppression. Parasitology. 2016 Gene Expression in Controlled Human Plasmodium falciparum Sep;143(10):1252-60. doi: 10.1017/S0031182016000809. Epub Infections. PLoS Pathog. 2016 Apr 12;12(4):e1005538. doi: 2016 May 18. 10.1371/journal.ppat.1005538. eCollection 2016 Apr.

Arnold MS, Engel JA, Chua MJ, Fisher GM, Skinner-Adams TS, Badman SG, Vallely LM, Toliman P, Kariwiga G, Lote B, Pomat W, Andrews KT. Adaptation of the [3H]Hypoxanthine Uptake Assay Holmer C, Guy R, Luchters S, Morgan C, Garland SM, Tabrizi S, for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites. Whiley D, Rogerson SJ, Mola G, Wand H, Donovan B, Causer L, Antimicrob Agents Chemother. 2016 Jun 20;60(7):4361-3. doi: Kaldor J, Vallely A. A novel point-of-care testing strategy for sexually 10.1128/AAC.02948-15. Print 2016 Jul. transmitted infections among pregnant women in high-burden settings: results of a feasibility study in Papua New Guinea. BMC Arumugam S, Wei J, Liu Z, Abraham D, Bell A, Bottazzi ME, Hotez Infect Dis. 2016 Jun 6;16:250. doi: 10.1186/s12879-016-1573-4. PJ, Zhan B, Lustigman S, Klei TR Vaccination of Gerbils with Bm- 103 and Bm-RAL-2 Concurrently or as a Fusion Protein Confers Banu SS, Meyer W, Ahmed BN, Kim R, Lee R. Detection of Consistent and Improved Protection against Brugia malayi Leishmania donovani in peripheral blood of asymptomatic Infection. PLoS Negl Trop Dis. 2016 Apr 5;10(4):e0004586. doi: individuals in contact with patients with visceral leishmaniasis. 10.1371/journal.pntd.0004586. eCollection 2016 Apr Trans R Soc Trop Med Hyg. 2016 May;110(5):286-93. doi: 10.1093/ trstmh/trw027. Atwell JE, Thumar B, Robinson LJ, Tobby R, Yambo P, Ome- Kaius M, Siba PM, Unger HW, Rogerson SJ, King CL, Karron RA. Baragaña B, Norcross NR, Wilson C, Porzelle A, Hallyburton I, Impact of Placental Malaria and Hypergammaglobulinemia on Grimaldi R, Osuna-Cabello M, Norval S, Riley J, Stojanovski L, Transplacental Transfer of Respiratory Syncytial Virus Antibody in Simeons FR, Wyatt PG, Delves MJ, Meister S, Duffy S,Avery VM, Papua New Guinea. J Infect Dis. 2016 Feb 1;213(3):423-31. doi: Winzeler EA, Sinden RE, Wittlin S, Frearson JA, Gray DW, Fairlamb 10.1093/infdis/jiv401. Epub 2015 Aug 3 AH, Waterson D, Campbell SF, Willis P, Read KD, Gilbert IH. Discovery of a Quinoline-4-carboxamide Derivative with a Novel Auburn S, Böhme U, Steinbiss S, Trimarsanto H, Hostetler J, Mechanism of Action, Multistage Antimalarial Activity, and Potent Sanders M, Gao Q, Nosten F, Newbold CI, Berriman M, Price RN, in Vivo Efficacy. J Med Chem. 2016 Nov 10;59(21):9672-9685. Otto TD. Molecular Epidemiology of P. vivax in Iran: High Diversity Epub 2016 Sep 10. and Complex Sub-Structure Using Neutral Markers, but No Evidence of Y976F Mutation at pvmdr1 Wellcome Open Res. 2016 Barber BE, Grigg MJ, William T, Yeo TW, Anstey NM. Intravascular Nov 15;1:4. doi: 10.12688/wellcomeopenres.9876.1 haemolysis with haemoglobinuria in a splenectomized patient with severe Plasmodium knowlesi malaria. Malar J. 2016 Sep 9;15:462. Auburn S, Serre D, Pearson RD, Amato R, Sriprawat K, To S, doi: 10.1186/s12936-016-1514-0. Handayuni I, Suwanarusk R, Russell B, Drury E, Stalker J, Miotto

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Barber BE, William T, Grigg MJ, Parameswaran U, Piera KA, Yeo Beeson JG, Fowkes FJ. A malaria vaccine in children with HIV. TW, Anstey NM. Asymmetric Dimethylarginine in Adult Falciparum Lancet Infect Dis. 2016 Oct;16(10):1087-9. doi: 10.1016/S1473- Malaria: Relationships With Disease Severity, Antimalarial 3099(16)30209-2. Epub 2016 Jul 7 Treatment, Hemolysis, and Inflammation. Open Forum Infect Dis. 2016 Feb 9;3(1):ofw027. doi: 10.1093/ofid/ofw027. eCollection Bennuru S, Cotton JA, Ribeiro JM, Grote A, Harsha B, Holroyd N, 2016 Jan. Mhashilkar A, Molina DM, Randall AZ, Shandling AD, Unnasch TR, Ghedin E, Berriman M, Lustigman S, Nutman TB. Stage-Specific Bargieri DY, Thiberge S, Tay CL, Carey AF, Rantz A, Hischen Transcriptome and Proteome Analyses of the Filarial Parasite F, Lorthiois A, Straschil U, Singh P, Singh S, Triglia T, Tsuboi T, Onchocerca volvulus and Its Wolbachia Endosymbiont. MBio. Cowman A, Chitnis C, Alano P, Baum J, Pradel G, Lavazec C, 2016 Nov 23;7(6). pii: e02028-16. doi: 10.1128/mBio.02028-16. Ménard R Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Berna AZ, McCarthy JS, Trowell SC. Malaria detection using breath Erythrocytes. Cell Host Microbe. 2016 Nov 9;20(5):618-630. doi: biomarkers. Med J Aust. 2016 Feb 1;204(2):50. 10.1016/j.chom.2016.10.015. Berry L, Chen CT, Reininger L, Carvalho TG, El Hajj H, Morlon- Barratt J, Chan D, Sandaradura I, Malik R, Spielman D, Lee Guyot J, Bordat Y, Lebrun M, Gubbels MJ, Doerig C, Daher W. R, Marriott D, Harkness J, Ellis J, Stark D. Angiostrongylus The conserved apicomplexan Aurora kinase TgArk3 is involved cantonensis: a review of its distribution, molecular biology and in endodyogeny, duplication rate and parasite virulence. Cell clinical significance as a human pathogen. Parasitology. 2016 Microbiol. 2016 Aug;18(8):1106-20. doi: 10.1111/cmi.12571. Aug;143(9):1087-118. doi: 10.1017/S0031182016000652. Epub Epub 2016 Feb 22. 2016 May 26. Bird EM, Parameswaran U, William T, Khoo TM, Grigg MJ, Barratt J, Gough R, Stark D, Ellis J. Bulky Trichomonad Aziz A, Marfurt J, Yeo TW, Auburn S, Anstey NM, Barber BE. Genomes: Encoding a Swiss Army Knife. Trends Parasitol. 2016 Transfusion-transmitted severe Plasmodium knowlesi malaria Oct;32(10):783-797. doi: 10.1016/j.pt.2016.05.014. Epub 2016 in a splenectomized patient with beta-thalassaemia major in Jun 13. Review. Sabah, Malaysia: a case report. Malar J. 2016 Jul 12;15(1):357. doi: 10.1186/s12936-016-1398-z. Barry A, Hansen D. Naturally acquired immunity to malaria. Parasitology. 2016 Feb;143(2):125-8. doi: 10.1017/ Boddey JA, O’Neill MT, Lopaticki S, Carvalho TG, Hodder AN, Nebl S0031182015001778. Epub 2016 Jan 8. T, Wawra S, van West P, Ebrahimzadeh Z, Richard D, Flemming S, Spielmann T, Przyborski J, Babon JJ, Cowman AF. Export of malaria Bassat Q, Velarde M, Mueller I, Lin J, Leslie T, Wongsrichanalai C, proteins requires co-translational processing of the PEXEL motif Baird JK. Key Knowledge Gaps for Plasmodium vivax Control and independent of phosphatidylinositol-3-phosphate binding. Nat Elimination. Am J Trop Med Hyg. 2016 Dec 28;95(6 Suppl):62-71. Commun. 2016 Feb 1;7:10470. doi: 10.1038/ncomms10470. doi: 10.4269/ajtmh.16-0180. Epub 2016 Jul 18. Bottomley C, Isham V, Vivas-Martínez S, Kuesel AC, Attah SK, Beeson JG, Drew DR, Boyle MJ, Feng G, Fowkes FJ, Richards JS. Opoku NO, Lustigman S, Walker M, Basáñez MG. Modelling Merozoite surface proteins in red blood cell invasion, immunity Neglected Tropical Diseases diagnostics: the sensitivity of skin and vaccines against malaria. FEMS Microbiol Rev. 2016 snips for Onchocerca volvulus in near elimination and surveillance May;40(3):343-72. doi: 10.1093/femsre/fuw001. Epub 2016 Jan settings. Parasit Vectors. 2016 Jun 14;9(1):343. doi: 10.1186/ 31. Review s13071-016-1605-3.

Page 54 Appendix 1

Bowen AC, Sheorey H, Bryant PA, Robson J, Burgner DP. Review. Polymerase chain reaction testing for faecal parasites: risks and alternatives. Med J Aust. 2016 Apr 18;204(7):262 Burel JG, Apte SH, Groves PL, Klein K, McCarthy JS, Doolan DL. Reduced Plasmodium Parasite Burden Associates with CD38+ Bowles VM, Yoon KS, Barker SC, Tran C, Rhodes C, Clark MJ. CD4+ T Cells Displaying Cytolytic Potential and Impaired IFN-γ Ovicidal Efficacy of Abametapir Against Eggs of Human Head and Production. PLoS Pathog. 2016 Sep 23;12(9):e1005839. doi: Body Lice (Anoplura: Pediculidae). J Med Entomol. 2016 Aug 21. 10.1371/journal.ppat.1005839. eCollection 2016 Sep. pii: tjw132. [Epub ahead of print] Erratum in: J Med Entomol. 2016 Nov 4;PMID: 27550081 Burel JG, Apte SH, McCarthy JS, Doolan DL. Plasmodium vivax but Not Plasmodium falciparum Blood-Stage Infection Britton S, Cheng Q, Grigg MJ, Poole CB, Pasay C, William T, in Humans Is Associated with the Expansion of a CD8+ T Cell Fornace K, Anstey NM, Sutherland CJ, Drakeley C, McCarthy JS. Population with Cytotoxic Potential. PLoS Negl Trop Dis. 2016 Sensitive Detection of Plasmodium vivax Using a High-Throughput, Dec 8;10(12):e0005031. doi: 10.1371/journal.pntd.0005031. Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) eCollection 2016 Dec. Platform: A Potential Novel Tool for Malaria Elimination. PLoS Negl Trop Dis. 2016 Feb 12;10(2):e0004443. doi: 10.1371/journal. Buskes MJ, Harvey KL, Prinz B, Crabb BS, Gilson PR, Wilson DJ, pntd.0004443. eCollection 2016 Feb. Abbott BM. Exploration of 3-methylisoquinoline-4-carbonitriles as protein kinase A inhibitors of Plasmodium falciparum. Britton S, Cheng Q, Grigg MJ, William T, Anstey NM, McCarthy Bioorg Med Chem. 2016 Jun 1;24(11):2389-96. doi: 10.1016/j. JS. A Sensitive, Colorimetric, High-Throughput Loop-Mediated bmc.2016.03.048. Epub 2016 Mar 28 Isothermal Amplification Assay for the Detection of Plasmodium knowlesi. Am J Trop Med Hyg. 2016 Jul 6;95(1):120-2. doi: Buskes MJ, Harvey KL, Richards BJ, Kalhor R, Christoff RM, Gardhi 10.4269/ajtmh.15-0670. Epub 2016 May 9. CK, Littler DR, Cope ED, Prinz B, Weiss GE, O’Brien NJ, Crabb BS, Deady LW, Gilson PR, Abbott BM. Antimalarial activity of novel Britton S, Cheng Q, McCarthy JS. Novel molecular diagnostic tools 4-cyano-3-methylisoquinoline inhibitors against Plasmodium for malaria elimination: a review of options from the point of view falciparum: design, synthesis and biological evaluation. Org of high-throughput and applicability in resource limited settings. Biomol Chem. 2016 May 18;14(20):4617-39. doi: 10.1039/ Malar J. 2016 Feb 16;15:88. doi: 10.1186/s12936-016-1158-0. c5ob02517f. Review. Cadavid Restrepo AM, Yang YR, McManus DP, Gray DJ, Burdam FH, Hakimi M, Thio F, Kenangalem E, Indrawanti R, Giraudoux P, Barnes TS, Williams GM, Soares Magalhães RJ, Noviyanti R, Trianty L, Marfurt J, Handayuni I, Soenarto Y, Douglas Hamm NA, Clements AC. The landscape epidemiology of NM, Anstey NM, Price RN, Poespoprodjo JR. Asymptomatic echinococcoses.Infect Dis Poverty. 2016 Feb 19;5:13. doi: Vivax and Falciparum Parasitaemia with Helminth Co-Infection: 10.1186/s40249-016-0109-x. Review. Major Risk Factors for Anaemia in Early Life. PLoS One. 2016 Aug 9;11(8):e0160917. doi: 10.1371/journal.pone.0160917. Cai P, Gobert GN, McManus DP. MicroRNAs in Parasitic eCollection 2016. Helminthiases: Current Status and Future Perspectives. Trends Parasitol. 2016 Jan;32(1):71-86. doi: 10.1016/j.pt.2015.09.003. Burel JG, Apte SH, Doolan DL. Systems Approaches towards Epub 2015 Oct 18. Review. Molecular Profiling of Human Immunity. Trends Immunol. 2016 Jan;37(1):53-67. doi: 10.1016/j.it.2015.11.006. Epub 2015 Dec 5. Cai P, Gobert GN, You H, McManus DP. The Tao survivorship

Page 55 Appendix 1

of schistosomes: implications for schistosomiasis control. Marriott D, Harkness J, Ellis J, Stark D. Detection of Dientamoeba Int J Parasitol. 2016 Jun;46(7):453-63. doi: 10.1016/j. fragilis in animal faeces using species specific real time PCR ijpara.2016.01.002. Epub 2016 Feb 9. Review. assay. Vet Parasitol. 2016 Aug 30;227:42-7. doi: 10.1016/j. vetpar.2016.07.025. Epub 2016 Jul 20. Cai P, Liu S, Piao X, Hou N, Gobert GN, McManus DP, Chen Q. Comprehensive Transcriptome Analysis of Sex-Biased Expressed Chan JA, Howell KB, Langer C, Maier AG, Hasang W, Rogerson Genes Reveals Discrete Biological and Physiological Features of SJ, Petter M, Chesson J, Stanisic DI, Duffy MF, Cooke BM, Siba Male and Female Schistosoma japonicum. PLoS Negl Trop Dis. PM, Mueller I, Bull PC, Marsh K, Fowkes FJ, Beeson JG. A single 2016 Apr 29;10(4):e0004684. doi: 10.1371/journal.pntd.0004684. point in protein trafficking by Plasmodium falciparum determines eCollection 2016 Apr. the expression of major antigens on the surface of infected erythrocytes targeted by human antibodies. Cell Mol Life Sci. 2016 Campbell B, Cortes H, Annoscia G, Giannelli A, Parisi A, Latrofa Nov;73(21):4141-58. doi: 10.1007/s00018-016-2267-1. Epub MS, Dantas-Torres F, Cardoso L, Otranto D. Paramyosin of canine 2016 May 19. Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease. Parasit Vectors. 2016 Sep 7;9(1):493. doi: Chandrasiri UP, Fowkes FJ, Beeson JG, Richards JS, Kamiza S, 10.1186/s13071-016-1783-z. Maleta K, Ashorn P, Rogerson SJ. Association between malaria immunity and pregnancy outcomes among Malawian pregnant Campbell SJ, Nery SV, Doi SA, Gray DJ, Soares Magalhães RJ, women receiving nutrient supplementation. Malar J. 2016 Nov McCarthy JS, Traub RJ, Andrews RM, Clements AC. Complexities 9;15(1):547. and Perplexities: A Critical Appraisal of the Evidence for Soil- Transmitted Helminth Infection-Related Morbidity. PLoS Negl Chandrudu S, Bartlett S, Khalil ZG, Jia Z, Hussein WM, Capon RJ, Trop Dis. 2016 May 19;10(5):e0004566. doi: 10.1371/journal. Batzloff MR,Good MF, Monteiro MJ, Skwarczynski M, Toth I. Linear pntd.0004566. eCollection 2016 May. Review. and branched polyacrylates as a delivery platform for peptide- based vaccines. Ther Deliv. 2016 Sep;7(9):601-9. doi: 10.4155/ Campbell SJ, Nery SV, McCarthy JS, Gray DJ, Soares Magalhães tde-2016-0037 RJ, Clements AC. A Critical Appraisal of Control Strategies for Soil- Transmitted Helminths. Trends Parasitol. 2016 Feb;32(2):97-107. Charnaud SC, McGready R, Herten-Crabb A, Powell R, Guy A, doi: 10.1016/j.pt.2015.10.006. Epub 2016 Jan 12. Review. Langer C, Richards JS, Gilson PR, Chotivanich K, Tsuboi T, Narum DL, Pimanpanarak M, Simpson JA, Beeson JG, Nosten F, Fowkes Carvalho TG, Morahan B, John von Freyend S, Boeuf P, Grau G, FJ. Maternal-foetal transfer of Plasmodium falciparum and Garcia-Bustos J, Doerig C. The ins and outs of phosphosignalling Plasmodium vivax antibodies in a low transmission setting. Sci Rep. in Plasmodium: Parasite regulation and host cell manipulation. 2016 Feb 10;6:20859. doi: 10.1038/srep20859 Mol Biochem Parasitol. 2016 Jul;208(1):2-15. doi: 10.1016/j. molbiopara.2016.05.006. Epub 2016 May 17 Chisholm SA, McHugh E, Lundie R, Dixon MW, Ghosh S, O’Keefe M, Tilley L, Kalanon M, de Koning-Ward TF. Contrasting Inducible Chambers RK, Khan TA, Olsen DB, Sleebs BE. Synthesis of amino Knockdown of the Auxiliary PTEX Component PTEX88 in P. heterocycle aspartyl protease inhibitors. Org Biomol Chem. 2016 falciparum and P. berghei Unmasks a Role in Parasite Virulence. Jun 14;14(22):4970-85. doi: 10.1039/c5ob01842k. Epub 2016 May PLoS One. 2016 Feb 17;11(2):e0149296. doi: 10.1371/journal. 4. pone.0149296. eCollection 2016.

Chan D, Barratt J, Roberts T, Phillips O, Šlapeta J, Ryan U, Chiu CY, White MT, Healer J, Thompson JK, Siba PM, Mueller

Page 56 Appendix 1

I, Cowman AF, Hansen DS. Different Regions of Plasmodium Cockburn IA, Zavala F. Dendritic cell function and antigen falciparum Erythrocyte-Binding Antigen 175 Induce Antibody presentation in malaria. Curr Opin Immunol. 2016 Jun;40:1-6. doi: Responses to Infection of Varied Efficacy. J Infect Dis. 2016 Jul 10.1016/j.coi.2016.01.010. Epub 2016 Feb 1. Review. 1;214(1):96-104. doi: 10.1093/infdis/jiw119. Epub 2016 Mar 28. Coffey MJ, Jennison C, Tonkin CJ, Boddey JA. Role of the ER and Chuah C, Jones MK, McManus DP, Nawaratna SK, Burke ML, Golgi in protein export by Apicomplexa. Curr Opin Cell Biol. 2016 Owen HC, Ramm GA, Gobert GN. Characterising granuloma Aug;41:18-24. doi: 10.1016/j.ceb.2016.03.007. Epub 2016 Mar regression and liver recovery in a murine model of schistosomiasis 25. Review. japonica. Int J Parasitol. 2016 Apr;46(4):239-52. doi: 10.1016/j. ijpara.2015.12.004. Epub 2016 Jan 23. Cotton JA, Bennuru S, Grote A, Harsha B, Tracey A, Beech R, Doyle SR, Dunn M, Hotopp JC, Holroyd N, Kikuchi T, Lambert O, Cissé B, Ba EH, Sokhna C, NDiaye JL, Gomis JF, Dial Y, Pitt C, NDiaye Mhashilkar A, Mutowo P, Nursimulu N, Ribeiro JM, Rogers MB, M, Cairns M, Faye E, NDiaye M, Lo A, Tine R, Faye S, Faye B, Sy Stanley E, Swapna LS, Tsai IJ, Unnasch TR, Voronin D, Parkinson J, O, Konate L, Kouevijdin E, Flach C, Faye O, Trape JF, Sutherland Nutman TB, Ghedin E, Berriman M, Lustigman S. The genome of C, Fall FB, Thior PM, Faye OK, Greenwood B, Gaye O, Milligan P. Onchocerca volvulus, agent of river blindness. Nat Microbiol. 2016 Effectiveness of Seasonal Malaria Chemoprevention in Children Nov 21;2:16216. doi: 10.1038/nmicrobiol.2016.216. under Ten Years of Age in Senegal: A Stepped-Wedge Cluster- Randomised Trial. PLoS Med. 2016 Nov 22;13(11):e1002175. doi: Cowman AF, Healer J, Marapana D, Marsh K. Malaria: Biology 10.1371/journal.pmed.1002175. eCollection 2016 Nov. and Disease. Cell. 2016 Oct 20;167(3):610-624. doi: 10.1016/j. cell.2016.07.055. Review. Clarke NE, Clements AC, Bryan S, McGown J, Gray D, Nery SV. Investigating the differential impact of school and community- Creek DJ, Chua HH, Cobbold SA, Nijagal B, MacRae JI, Dickerman based integrated control programmes for soil-transmitted BK, Gilson PR, Ralph SA, McConville MJ. Metabolomics-Based helminths in Timor-Leste: the (S)WASH-D for Worms pilot study Screening of the Malaria Box Reveals both Novel and Established protocol. Pilot Feasibility Stud. 2016 Dec 8;2:69. eCollection 2016. Mechanisms of Action. Antimicrob Agents Chemother. 2016 Oct 21;60(11):6650-6663. doi: 10.1128/AAC.01226-16. Print 2016 Cobbold SA, Chua HH, Nijagal B, Creek DJ, Ralph SA, Nov. McConville MJ. Metabolic Dysregulation Induced in Plasmodium falciparum by Dihydroartemisinin and Other Front-Line Curtis MP, Vaillancourt V, Goodwin RM, Chubb NA, Howson W, Antimalarial Drugs. J Infect Dis. 2016 Jan 15;213(2):276-86. doi: McTier TL, Pullins A, Zinser EW, Meeus PF, Woods DJ, Hedges 10.1093/infdis/jiv372. Epub 2015 Jul 6. L, Stuk T, Price JE, Koch JD, Menon SR. Design and synthesis of sarolaner, a novel, once-a-month, oral isoxazoline for the control Cobbold SA, Llinás M, Kirk K. Sequestration and metabolism of fleas and ticks on dogs. Bioorg Med Chem Lett. 2016 Apr of host cell arginine by the intraerythrocytic malaria parasite 1;26(7):1831-5. doi: 10.1016/j.bmcl.2016.02.027. Epub 2016 Feb Plasmodium falciparum. Cell Microbiol. 2016 Jun;18(6):820-30. 12. doi: 10.1111/cmi.12552. Epub 2016 Jan 6. de Assis RR, Ludolf F, Nakajima R, Jasinskas A, Oliveira GC, Felgner Cobbold SA, Santos JM, Ochoa A, Perlman DH, Llinás M. PL, Gaze ST, Loukas A, LoVerde PT, Bethony JM, Correa-Oliveira Proteome-wide analysis reveals widespread lysine acetylation of R, Calzavara-Silva CE. A next-generation proteome array for major protein complexes in the malaria parasite. Sci Rep. 2016 Jan Schistosoma mansoni. Int J Parasitol. 2016 Jun;46(7):411-5. doi: 27;6:19722. doi: 10.1038/srep19722. 10.1016/j.ijpara.2016.04.001. Epub 2016 Apr 27.

Page 57 Appendix 1

de Koning-Ward TF, Dixon MW, Tilley L, Gilson PR. Plasmodium Donald W, Pasay C, Guintran JO, Iata H, Anderson K, Nausien species: master renovators of their host cells. Nat Rev Microbiol. J, Gresty KJ, Waters NC, Vestergaard LS, Taleo G, Cheng Q. The 2016 Aug;14(8):494-507. doi: 10.1038/nrmicro.2016.79. Epub Utility of Malaria Rapid Diagnostic Tests as a Tool in Enhanced 2016 Jul 4. Review. Surveillance for Malaria Elimination in Vanuatu. PLoS One. 2016 Nov 30;11(11):e0167136. doi: 10.1371/journal.pone.0167136. De SL, Stanisic DI, Rivera F, Batzloff MR, Engwerda C,Good MF. eCollection 2016. Plasmodium berghei bio-burden correlates with parasite lactate dehydrogenase: application to murine Plasmodium diagnostics. Drew DR, Wilson DW, Elliott SR, Cross N, Terheggen U, Hodder Malar J. 2016 Jan 4;15:3. doi: 10.1186/s12936-015-1027-2 AN, Siba PM, Chelimo K, Dent AE, Kazura JW, Mueller I, Beeson JG. A novel approach to identifying patterns of human invasion- De SL, Stanisic DI, van Breda K, Bellete B, Harris I, McCallum inhibitory antibodies guides the design of malaria vaccines F, Edstein MD, Good MF. Persistence and immunogenicity of incorporating polymorphic antigens. BMC Med. 2016 Sep chemically attenuated blood stage Plasmodium falciparum in 23;14(1):144. Aotus monkeys. Int J Parasitol. 2016 Aug;46(9):581-91. doi: 10.1016/j.ijpara.2016.05.002. Epub 2016 May 26 Driguez P, Li Y, Gaze S, Pearson MS, Nakajima R, Trieu A, Doolan DL, Felgner PL, Hou X, Cardoso FC, Jasinskas A, Gobert GN, De Sousa KP, Doolan DL. Immunomics: a 21st century approach Loukas A, McManus DP. Antibody Signatures Reflect Different to vaccine development for complex pathogens. Parasitology. 2016 Disease Pathologies in Patients With Schistosomiasis Due to Feb;143(2):236-44. doi: 10.1017/S0031182015001079. Review. Schistosoma japonicum. J Infect Dis. 2016 Jan 1;213(1):122-30. doi: 10.1093/infdis/jiv356. Epub 2015 Jul 6. Dearnley M, Chu T, Zhang Y, Looker O, Huang C, Klonis N, Yeoman J, Kenny S, Arora M, Osborne JM, Chandramohanadas R, Zhang S, Driguez P, McManus DP, Gobert GN. Clinical implications of Dixon MW, Tilley L. Reversible host cell remodeling underpins recent findings in schistosome proteomics. Expert Rev Proteomics. deformability changes in malaria parasite sexual blood stages. Proc 2016;13(1):19-33. doi: 10.1586/14789450.2016.1116390. Epub Natl Acad Sci U S A. 2016 Apr 26;113(17):4800-5. doi: 10.1073/ 2015 Nov 26. Review. pnas.1520194113. Epub 2016 Apr 11. Driguez P, McWilliam HE, Gaze S, Piedrafita D, Pearson MS, Decuypere S, Maltha J, Deborggraeve S, Rattray NJ, Issa G, Nakajima R, Duke M, Trieu A, Doolan DL, Cardoso FC, Jasinskas Bérenger K, Lompo P, Tahita MC, Ruspasinghe T, McConville A, Gobert GN, Felgner PL, Loukas A, Meeusen E, McManus DP. M, Goodacre R, Tinto H, Jacobs J, Carapetis JR. Towards Specific humoral response of hosts with variable schistosomiasis Improving Point-of-Care Diagnosis of Non-malaria Febrile susceptibility. Immunol Cell Biol. 2016 Jan;94(1):52-65. doi: Illness: A Metabolomics Approach. PLoS Negl Trop Dis. 2016 10.1038/icb.2015.61. Epub 2015 Jun 5. Mar 4;10(3):e0004480. doi: 10.1371/journal.pntd.0004480. eCollection 2016 Mar. Drinkwater N, Vinh NB, Mistry SN, Bamert RS, Ruggeri C, Holleran J P, Loganathan S, Paiardini A, Charman SA, Powell AK, Avery VM, Dickerman BK, Elsworth B, Cobbold SA, Nie CQ, McConville MJ, McGowan S, Scammells PJ. Potent dual inhibitors of Plasmodium Crabb BS, Gilson PR Identification of inhibitors that dually target falciparum M1 and M17 aminopeptidases through optimization of the new permeability pathway and dihydroorotate dehydrogenase S1 pocket interactions. Eur J Med Chem. 2016 Mar 3;110:43-64. in the blood stage of Plasmodium falciparum. Sci Rep. 2016 Nov doi: 10.1016/j.ejmech.2016.01.015. Epub 2016 Jan 13. 22;6:37502. doi: 10.1038/srep37502. Duffy MF, Noviyanti R, Tsuboi T, Feng ZP, Trianty L, Sebayang BF,

Page 58 Appendix 1

Takashima E, Sumardy F, Lampah DA, Turner L, Lavstsen T, Fowkes CR. Combined Immune Therapy for the Treatment of Visceral FJ, Siba P, Rogerson SJ, Theander TG, Marfurt J, Price RN, Anstey Leishmaniasis. PLoS Negl Trop Dis. 2016 Feb 12;10(2):e0004415. NM, Brown GV, Papenfuss AT. Differences in PfEMP1s recognized doi: 10.1371/journal.pntd.0004415. eCollection 2016 Feb. by antibodies from patients with uncomplicated or severe malaria. Malar J. 2016 May 5;15(1):258. doi: 10.1186/s12936-016-1296-4. Fang F, Candy K, Melloul E, Bernigaud C, Chai L, Darmon C, Durand R, Botterel F, Chosidow O, Izri A, Huang W, Guillot J. In Duffy S, Loganathan S,Holleran JP, Avery VM. Large-scale vitro activity of ten essential oils against Sarcoptes scabiei. Parasit production of Plasmodium falciparum gametocytes for malaria Vectors. 2016 Nov 22;9(1):594. drug discovery. Nat Protoc. 2016 May;11(5):976-92. doi: 10.1038/ nprot.2016.056. Epub 2016 Apr 28. Farrington LA, Jagannathan P, McIntyre TI, Vance HM, Bowen K, Boyle MJ, Nankya F, Wamala S, Auma A, Nalubega M, Sikyomu Ebine K, Hirai M, Sakaguchi M, Yahata K, Kaneko O, Saito-Nakano E, Naluwu K, Bigira V, Kapisi J, Dorsey G, Kamya MR, Feeney ME. Y. Plasmodium Rab5b is secreted to the cytoplasmic face of the Frequent Malaria Drives Progressive Vδ2 T-Cell Loss, Dysfunction, tubovesicular network in infected red blood cells together with and CD16 Up-regulation During Early Childhood. J Infect Dis. 2016 N-acylated adenylate kinase 2. Malar J. 2016 Jun 17;15:323. doi: May 1;213(9):1483-90. doi: 10.1093/infdis/jiv600. Epub 2015 Dec 10.1186/s12936-016-1377-4. 13.

Elsworth B, Sanders PR, Nebl T, Batinovic S, Kalanon M, Nie CQ, Fernandez-Ruiz D, Ng WY, Holz LE, Ma JZ, Zaid A, Wong YC, Lau Charnaud SC, Bullen HE, de Koning Ward TF, Tilley L, Crabb BS, LS, Mollard V, Cozijnsen A, Collins N, Li J, Davey GM, Kato Y, Devi Gilson PR. Proteomic analysis reveals novel proteins associated S, Skandari R, Pauley M, Manton JH, Godfrey DI, Braun A, Tay SS, with the Plasmodium protein exporter PTEX and a loss of complex Tan PS, Bowen DG, Koch-Nolte F, Rissiek B, Carbone FR, Crabb stability upon truncation of the core PTEX component, PTEX150. BS, Lahoud M, Cockburn IA, Mueller SN, Bertolino P, McFadden Cell Microbiol. 2016 Nov;18(11):1551-1569. doi: 10.1111/ GI, Caminschi I, Heath WR. Liver-Resident Memory CD8+ T cmi.12596. Epub 2016 May 3. Cells Form a Front-Line Defense against Malaria Liver-Stage Infection. Immunity. 2016 Oct 18;45(4):889-902. doi: 10.1016/j. Engelman D, Fuller LC, Solomon AW, McCarthy JS, Hay RJ, Lammie immuni.2016.08.011. Epub 2016 Sep 27. PJ, Steer AC Opportunities for Integrated Control of Neglected Tropical Diseases That Affect the Skin. Trends Parasitol. 2016 Fletcher S, Lucantoni L, Sykes ML, Jones AJ, Holleran JP, Saliba Nov;32(11):843-854. doi: 10.1016/j.pt.2016.08.005. Epub 2016 KJ, Avery VM. Biological characterization of chemically diverse Sep 13. Review. compounds targeting the Plasmodium falciparum coenzyme A synthesis pathway. Parasit Vectors. 2016 Nov 17;9(1):589. Espino FE, Bibit JA, Sornillo JB, Tan A, von Seidlein L, Ley B. Comparison of Three Screening Test Kits for G6PD Enzyme Fontana MF, Baccarella A, Craft JF, Boyle MJ, McIntyre TI, Deficiency: Implications for Its Use in the Radical Cure of Vivax Wood MD, Thorn KS, Anidi C, Bayat A, Chung MR, Hamburger Malaria in Remote and Resource-Poor Areas in the Philippines. R, Kim CY, Pearman E, Pham J, Tang JJ, Boon L, Kamya MR, PLoS One. 2016 Feb 5;11(2):e0148172. doi: 10.1371/journal. Dorsey G, Feeney ME, Kim CC. A Novel Model of Asymptomatic pone.0148172. eCollection 2016. Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection. PLoS One. 2016 Faleiro RJ, Kumar R, Bunn PT, Singh N, Chauhan SB, Sheel M, Sep 1;11(9):e0162132. doi: 10.1371/journal.pone.0162132. Amante FH, Montes de Oca M, Edwards CL, Ng SS, Best SE, Haque eCollection 2016. A, Beattie L, Hafner LM, Sacks D, Nylen S, Sundar S, Engwerda

Page 59 Appendix 1

Fornace KM, Abidin TR, Alexander N, Brock P, Grigg MJ, Leishmaniasis in the Absence of TNF Despite a Strong Th1 Murphy A, William T, Menon J, Drakeley CJ, Cox J. Association Response. Front Microbiol. 2016 Jan 22;6:1520. doi: 10.3389/ between Landscape Factors and Spatial Patterns of Plasmodium fmicb.2015.01520. eCollection 2015. knowlesi Infections in Sabah, Malaysia. Emerg Infect Dis. 2016 Feb;22(2):201-8. doi: 10.3201/eid2202.150656. Fuaad AA, Pearson MS, Pickering DA, Becker L, Zhao G, Loukas AC, Skwarczynski M, Toth I. Lipopeptide Nanoparticles: Fornace KM, Nuin NA, Betson M, Grigg MJ, William T, Anstey Development of Vaccines against Hookworm Parasite. NM, Yeo TW, Cox J, Ying LT, Drakeley CJ. Asymptomatic and ChemMedChem. 2015 Oct;10(10):1647-54. doi: 10.1002/ Submicroscopic Carriage of Plasmodium knowlesi Malaria in cmdc.201500227. Epub 2015 Aug 12. Household and Community Members of Clinical Cases in Sabah, Malaysia. J Infect Dis. 2016 Mar 1;213(5):784-7. doi: 10.1093/ Gazdik M, Jarman KE, O’Neill MT, Hodder AN, Lowes KN, Jousset infdis/jiv475. Epub 2015 Oct 3. Sabroux H, Cowman AF, Boddey JA, Sleebs BE. Exploration of the P3 region of PEXEL peptidomimetics leads to a potent inhibitor of Fowkes FJ, Boeuf P, Beeson JG. Immunity to malaria in an era of the Plasmodium protease, plasmepsin V. Bioorg Med Chem. 2016 declining malaria transmission. Parasitology. 2016 Feb;143(2):139- May 1;24(9):1993-2010. doi: 10.1016/j.bmc.2016.03.027. Epub 53. doi: 10.1017/S0031182015001249. Epub 2016 Jan 7. Review. 2016 Mar 16.

França CT, He WQ, Gruszczyk J, Lim NT, Lin E, Kiniboro B, Siba Gelabert P, Sandoval-Velasco M, Olalde I, Fregel R, Rieux A, Escosa PM, Tham WH, Mueller I. Plasmodium vivax Reticulocyte R, Aranda C, Paaijmans K, Mueller I, Gilbert MT, Lalueza-Fox C. Binding Proteins Are Key Targets of Naturally Acquired Immunity Mitochondrial DNA from the eradicated European Plasmodium in Young Papua New Guinean Children. PLoS Negl Trop Dis. 2016 vivax and P. falciparum from 70-year-old slides from the Ebro Delta Sep 27;10(9):e0005014. doi: 10.1371/journal.pntd.0005014. in Spain. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11495- eCollection 2016 Sep. 11500. Epub 2016 Sep 26.

França CT, Hostetler JB, Sharma S, White MT, Lin E, Kiniboro B, Giacomin P, Agha Z, Loukas A. Helminths and Intestinal Flora Team Waltmann A, Darcy AW, Li Wai Suen CS, Siba P, King CL, Rayner Up to Improve Gut Health. Trends Parasitol. 2016 Sep;32(9):664-6. JC, Fairhurst RM, Mueller I. An Antibody Screen of a Plasmodium doi: 10.1016/j.pt.2016.05.006. Epub 2016 May 24. vivax Antigen Library Identifies Novel Merozoite Proteins Associated with Clinical Protection. PLoS Negl Trop Dis. 2016 Giacomin P, Zakrzewski M, Jenkins TP, Su X, Al-Hallaf R, Croese May 16;10(5):e0004639. doi: 10.1371/journal.pntd.0004639. J, de Vries S, Grant A, Mitreva M, Loukas A, Krause L, Cantacessi eCollection 2016 May. C. Changes in duodenal tissue-associated microbiota following hookworm infection and consecutive gluten challenges in humans Frempong KK, Walker M, Cheke RA, Tetevi EJ, Gyan ET, Owusu with coeliac disease. Sci Rep. 2016 Nov 9;6:36797. doi: 10.1038/ EO, Wilson MD, Boakye DA, Taylor MJ, Biritwum NK, Osei- srep36797. Atweneboana M, Basáñez MG. Does Increasing Treatment Frequency Address Suboptimal Responses to Ivermectin for the Giddam AK, Reiman JM, Zaman M, Skwarczynski M, Toth I, Good Control and Elimination of River Blindness? Clin Infect Dis. 2016 MF. A semi-synthetic whole parasite vaccine designed to protect Jun 1;62(11):1338-47. doi: 10.1093/cid/ciw144. Epub 2016 Mar against blood stage malaria. Acta Biomater. 2016 Oct 15;44:295- 21. 303. doi: 10.1016/j.actbio.2016.08.020. Epub 2016 Aug 17.

Fromm PD, Kling JC, Remke A, Bogdan C, Körner H. Fatal Gilson PR, Chisholm SA, Crabb BS, de Koning-Ward TF. Host

Page 60 Appendix 1

cell remodelling in malaria parasites: a new pool of potential drug Khoury D, Cromer D, Davenport M, Sattabongkot J, Ivinson targets. Int J Parasitol. 2017 Feb;47(2-3):119-127. doi: 10.1016/j. K, Ockenhouse C, McCarthy J. Safety and Reproducibility of a ijpara.2016.06.001. Epub 2016 Jun 29. Review. Clinical Trial System Using Induced Blood Stage Plasmodium vivax Infection and Its Potential as a Model to Evaluate Malaria Good MF, Yanow SK. Cryptic epitope for antibodies should Transmission. PLoS Negl Trop Dis. 2016 Dec 8;10(12):e0005139. not be forgotten in vaccine design. Expert Rev Vaccines. 2016 doi: 10.1371/journal.pntd.0005139. eCollection 2016 Dec. Jun;15(6):675-6. doi: 10.1586/14760584.2016.1154791. Epub 2016 Mar 21 Grigg MJ, Barber BE, Marfurt J, Imwong M, William T, Bird E, Piera KA, Aziz A, Boonyuen U, Drakeley CJ, Cox J, White NJ, Cheng Q, Yeo Goodman CD, Austarheim I, Mollard V, Mikolo B, Malterud TW, Auburn S, Anstey NM. Dihydrofolate-Reductase Mutations in KE, McFadden GI, Wangensteen H. Natural products from Plasmodium knowlesi Appear Unrelated to Selective Drug Pressure Zanthoxylum heitzii with potent activity against the malaria from Putative Human-To-Human Transmission in Sabah, Malaysia. parasite. Malar J. 2016 Sep 20;15:481. doi: 10.1186/s12936-016- PLoS One. 2016 Mar 1;11(3):e0149519. doi: 10.1371/journal. 1533-x. Erratum in: Malar J. 2016 Nov 17;15(1):560. pone.0149519. eCollection 2016.

Goodman CD, Pasaje CF, Kennedy K, McFadden GI, Ralph SA. Grigg MJ, William T, Menon J, Barber BE, Wilkes CS, Rajahram GS, Targeting Protein Translation in Organelles of the Apicomplexa. Edstein MD, Auburn S, Price RN, Yeo TW, Anstey NM. Efficacy of Trends Parasitol. 2016 Dec;32(12):953-965. doi: 10.1016/j. Artesunate-mefloquine for Chloroquine-resistant Plasmodium pt.2016.09.011. Epub 2016 Oct 25. Review. vivax Malaria in Malaysia: An Open-label, Randomized, Controlled Trial. Clin Infect Dis. 2016 Jun 1;62(11):1403-11. doi: 10.1093/cid/ Goodman CD, Siregar JE, Mollard V, Vega-Rodríguez J, Syafruddin ciw121. Epub 2016 Apr 22. D, Matsuoka H, Matsuzaki M, Toyama T, Sturm A, Cozijnsen A, Jacobs-Lorena M, Kita K, Marzuki S, McFadden GI. Parasites Grigg MJ, William T, Menon J, Dhanaraj P, Barber BE, Wilkes CS, resistant to the antimalarial atovaquone fail to transmit by von Seidlein L, Rajahram GS, Pasay C, McCarthy JS, Price RN, mosquitoes. Science. 2016 Apr 15;352(6283):349-53. doi: Anstey NM, Yeo TW. Artesunate-mefloquine versus chloroquine 10.1126/science.aad9279. for treatment of uncomplicated Plasmodium knowlesi malaria in Malaysia (ACT KNOW): an open-label, randomised controlled trial. Govindasamy G, Barber BE, Ghani SA, William T, Grigg MJ, Lancet Infect Dis. 2016 Feb;16(2):180-8. doi: 10.1016/S1473- Borooah S, Dhillon B, Dondorp AM, Yeo TW, Anstey NM, Maude 3099(15)00415-6. Epub 2015 Nov 19. RJ. Retinal Changes in Uncomplicated and Severe Plasmodium knowlesi Malaria. J Infect Dis. 2016 May 1;213(9):1476-82. doi: Gruszczyk J, Lim NT, Arnott A, He WQ, Nguitragool W, Roobsoong 10.1093/infdis/jiv746. Epub 2015 Dec 15. W, Mok YF, Murphy JM, Smith KR, Lee S, Bahlo M, Mueller I, Barry AE, Tham WH. Structurally conserved erythrocyte-binding domain Gray KA, Gresty KJ, Chen N, Zhang V, Gutteridge CE, Peatey in Plasmodium provides a versatile scaffold for alternate receptor CL, Chavchich M, Waters NC, Cheng Q. Correlation between engagement. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):E191- Cyclin Dependent Kinases and Artemisinin-Induced Dormancy 200. doi: 10.1073/pnas.1516512113. Epub 2015 Dec 29. in Plasmodium falciparum In Vitro. PLoS One. 2016 Jun 21;11(6):e0157906. doi: 10.1371/journal.pone.0157906. Guan J, Hachey M, Puri L, Howieson V, Saliba KJ, Auclair K. A eCollection 2016. cross-metathesis approach to novel pantothenamide derivatives. Beilstein J Org Chem. 2016 May 13;12:963-8. doi: 10.3762/ Griffin P, Pasay C, Elliott S, Sekuloski S, Sikulu M, Hugo L, bjoc.12.95. eCollection 2016.

Page 61 Appendix 1

Hamedi Y, Sharifi-Sarasiabi K, Dehghan F, Safari R,To S, B, Hill AV, Marsh K, Draper SJ. Changes in Serological Immunology Handayuni I, Trimarsanto H, Price RN, Auburn S. Molecular Measures in UK and Kenyan Adults Post-controlled Human Malaria Epidemiology of P. vivax in Iran: High Diversity and Complex Infection. Front Microbiol. 2016 Oct 13;7:1604. eCollection 2016. Sub-Structure Using Neutral Markers, but No Evidence of Y976F Mutation at pvmdr1.PLoS One. 2016 Nov 9;11(11):e0166124. doi: Horne-Debets JM, Karunarathne DS, Faleiro RJ, Poh CM, Renia L, 10.1371/journal.pone.0166124. eCollection 2016. Wykes MN. Mice lacking Programmed cell death-1 show a role for CD8(+) T cells in long-term immunity against blood-stage malaria. Hess JA, Zhan B, Torigian AR, Patton JB, Petrovsky N, Zhan T, Sci Rep. 2016 May 24;6:26210. doi: 10.1038/srep26210. Bottazzi ME, Hotez PJ, Klei TR, Lustigman S, Abraham D. The Immunomodulatory Role of Adjuvants in Vaccines Formulated Hortle E, Nijagal B, Bauer DC, Jensen LM, Ahn SB, Cockburn with the Recombinant Antigens Ov-103 and Ov-RAL-2 against IA, Lampkin S, Tull D, McConville MJ, McMorran BJ, Foote SJ, Onchocerca volvulus in Mice. PLoS Negl Trop Dis. 2016 Jul Burgio G. Adenosine monophosphate deaminase 3 activation 7;10(7):e0004797. doi: 10.1371/journal.pntd.0004797. shortens erythrocyte half-life and provides malaria resistance eCollection 2016 Jul. in mice. Blood. 2016 Sep 1;128(9):1290-301. doi: 10.1182/ blood-2015-09-666834. Epub 2016 Jul 27. Hetzel MW, Reimer LJ, Gideon G, Koimbu G, Barnadas C, Makita L, Siba PM, Mueller I Changes in malaria burden and transmission Hotez PJ, Strych U, Lustigman S, Bottazzi ME. Human in sentinel sites after the roll-out of long-lasting insecticidal nets anthelminthic vaccines: Rationale and challenges. Vaccine. 2016 in Papua New Guinea. Parasit Vectors. 2016 Jun 14;9(1):340. doi: Jun 24;34(30):3549-55. doi: 10.1016/j.vaccine.2016.03.112. Epub 10.1186/s13071-016-1635-x 2016 May 9.

Hickey BW, Lumsden JM, Reyes S, Sedegah M, Hollingdale MR, Howieson VM, Tran E, Hoegl A, Fam HL, Fu J, Sivonen K, Freilich DA, Luke TC, Charoenvit Y, Goh LM, Berzins MP, Bebris Li XX, Auclair K, Saliba KJ. Triazole Substitution of a Labile L, Sacci JB Jr, De La Vega P, Wang R, Ganeshan H, Abot EN, Amide Bond Stabilizes Pantothenamides and Improves Their Carucci DJ, Doolan DL, Brice GT, Kumar A, Aguiar J, Nutman Antiplasmodial Potency. Antimicrob Agents Chemother. 2016 Nov TB, Leitman SF, Hoffman SL, Epstein JE, Richie TL. Mosquito bite 21;60(12):7146-7152. Print 2016 Dec. immunization with radiation-attenuated Plasmodium falciparum sporozoites: safety, tolerability, protective efficacy and humoral Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote immunogenicity. Malar J. 2016 Jul 22;15(1):377. doi: 10.1186/ SJ, Burgio G. A novel ENU-induced ankyrin-1 mutation impairs s12936-016-1435-y. parasite invasion and increases erythrocyte clearance during malaria infection in mice. Sci Rep. 2016 Nov 16;6:37197. doi: Hill DL, Wilson DW, Sampaio NG, Eriksson EM, Ryg-Cornejo V, 10.1038/srep37197. Harrison GL, Uboldi AD, Robinson LJ, Beeson JG, Siba P, Cowman AF, Hansen DS, Mueller I, Schofield L. Merozoite Antigens of Hupalo DN, Barnadas C, Barry A, Mueller I et al Population Plasmodium falciparum Elicit Strain-Transcending Opsonizing genomics studies identify signatures of global dispersal and drug Immunity. Infect Immun. 2016 Jul 21;84(8):2175-84. doi: 10.1128/ resistance in Plasmodium vivax. Nat Genet. 2016 Aug;48(8):953-8. IAI.00145-16. Print 2016 Aug. doi: 10.1038/ng.3588. Epub 2016 Jun 27.

Hodgson SH, Llewellyn D, Silk SE, Milne KH, Elias SC, Miura K, Ioannidis LJ, Nie CQ, Ly A, Ryg-Cornejo V, Chiu CY, Hansen DS. Kamuyu G, Juma EA, Magiri C, Muia A, Jin J, Spencer AJ, Longley Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient RJ, Mercier T, Decosterd L, Long CA, Osier FH, Hoffman SL, Ogutu Control of Blood-Stage Malaria Infection and Promotes Severe

Page 62 Appendix 1

Disease. J Immunol. 2016 Feb 1;196(3):1227-38. doi: 10.4049/ Karunarathne DS, Horne-Debets JM, Huang JX, Faleiro R, Leow jimmunol.1501562. Epub 2015 Dec 30. CY, Amante F, Watkins TS, Miles JJ, Dwyer PJ, Stacey KJ, Yarski M, Poh CM, Lee JS, Cooper MA, Rénia L, Richard D, McCarthy JS, Jagannathan P, Bowen K, Nankya F, McIntyre TI, Auma A, Wamala Sharpe AH, Wykes MN Programmed Death-1 Ligand 2-Mediated S, Sikyomu E, Naluwu K, Nalubega M, Boyle MJ, Farrington LA, Regulation of the PD-L1 to PD-1 Axis Is Essential for Establishing Bigira V, Kapisi J, Aweeka F, Greenhouse B, Kamya M, Dorsey G, CD4(+) T Cell Immunity. Immunity. 2016 Aug 16;45(2):333-45. doi: Feeney ME. Effective Antimalarial Chemoprevention in Childhood 10.1016/j.immuni.2016.07.017. Enhances the Quality of CD4+ T Cells and Limits Their Production of Immunoregulatory Interleukin 10. J Infect Dis. 2016 Jul Kato N, Avery VM et al Diversity-oriented synthesis yields 15;214(2):329-38. doi: 10.1093/infdis/jiw147. Epub 2016 Apr 10. novel multistage antimalarial inhibitors. Nature. 2016 Oct 20;538(7625):344-349. doi: 10.1038/nature19804. Epub 2016 Johnson S, Rahmani R, Drew DR, Williams MJ, Wilkinson M, Tan Sep 7. YH, Huang JX, Tonkin CJ, Beeson JG, Baum J, Smith BJ, Baell JB. Truncated Latrunculins as Actin Inhibitors Targeting Plasmodium Kennedy AT, Schmidt CQ, Thompson JK, Weiss GE, falciparum Motility and Host Cell Invasion. J Med Chem. 2016 Dec Taechalertpaisarn T, Gilson PR, Barlow PN, Crabb BS, Cowman 22;59(24):10994-11005. doi: 10.1021/acs.jmedchem.6b01109. AF, Tham WH. Recruitment of Factor H as a Novel Complement Epub 2016 Dec 2. Evasion Strategy for Blood-Stage Plasmodium falciparum Infection. J Immunol. 2016 Feb 1;196(3):1239-48. doi: 10.4049/ Kalanon M, Bargieri D, Sturm A, Matthews K, Ghosh S, Goodman jimmunol.1501581. Epub 2015 Dec 23. CD, Thiberge S, Mollard V, McFadden GI, Ménard R, de Koning- Ward TF. The Plasmodium translocon of exported proteins Khalil I, Tedla, BA et al Burden of Diarrhea in the Eastern component EXP2 is critical for establishing a patent malaria Mediterranean Region, 1990-2013: Findings from the Global infection in mice. Cell Microbiol. 2016 Mar;18(3):399-412. doi: Burden of Disease Study 2013. Am J Trop Med Hyg. 2016 Dec 10.1111/cmi.12520. Epub 2015 Oct 14. 7;95(6):1319-1329. Epub 2016 Oct 10.

Karl S, Laman M, Moore BR, Benjamin JM, Salib M, Lorry L, Khan NH, Bari AU, Hashim R, Khan I, Muneer A, Shah A, Wahid Maripal S, Siba P, Robinson LJ, Mueller I, Davis TM. Risk factors S, Yardley V, O’Neil B, Sutherland CJ. Cutaneous Leishmaniasis for Plasmodium falciparum and Plasmodium vivax gametocyte in Khyber Pakhtunkhwa Province of Pakistan: Clinical Diversity carriage in Papua New Guinean children with uncomplicated and Species-Level Diagnosis. Am J Trop Med Hyg. 2016 Nov malaria. Acta Trop. 2016 Aug;160:1-8. doi: 10.1016/j. 2;95(5):1106-1114. Epub 2016 Sep 6. actatropica.2016.04.002. Epub 2016 Apr 4. Kho S, Marfurt J, Handayuni I, Pava Z, Noviyanti R, Kusuma A, Karl S, White MT, Milne GJ, Gurarie D, Hay SI, Barry AE, Felger Piera KA, Burdam FH, Kenangalem E, Lampah DA, Engwerda CR, I, Mueller I. Spatial Effects on the Multiplicity of Plasmodium Poespoprodjo JR, Price RN, Anstey NM, Minigo G, Woodberry falciparum Infections. PLoS One. 2016 Oct 6;11(10):e0164054. T. Characterization of blood dendritic and regulatory T cells doi: 10.1371/journal.pone.0164054. eCollection 2016. in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. Malar J. 2016 Jun Karunajeewa HA, Mueller I. How important is gametocyte 21;15:328. doi: 10.1186/s12936-016-1382-7. clearance after malaria therapy? BMC Med. 2016 Jun 18;14:93. doi: 10.1186/s12916-016-0641-3. Khoury DS, Cromer D, Möhrle JJ, McCarthy JS, Davenport MP. Defining the Effectiveness of Antimalarial Chemotherapy:

Page 63 Appendix 1

Investigation of the Lag in Parasite Clearance Following Drug of Activity and Dose-Efficacy Modeling. PLoS Med. 2016 Oct Administration. J Infect Dis. 2016 Sep 1;214(5):753-61. doi: 4;13(10):e1002138. doi: 10.1371/journal.pmed.1002138. 10.1093/infdis/jiw234. Epub 2016 May 31. eCollection 2016 Oct.

Kim JY, Goo YK, Zo YG, Ji SY, Trimarsanto H, To S, Clark TG, Lelliott PM, Coban C. IFN-γ protects hepatocytes against Price RN, Auburn S. Further Evidence of Increasing Diversity Plasmodium vivax infection via LAP-like degradation of of Plasmodium vivax in the Republic of Korea in Recent Years. sporozoites. Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6813-5. PLoS One. 2016 Mar 18;11(3):e0151514. doi: 10.1371/journal. doi: 10.1073/pnas.1607007113. Epub 2016 Jun 10 pone.0151514. eCollection 2016 Ley B, Alam MS, Thriemer K, Hossain MS, Kibria MG, Auburn S, Koehler AV, Spratt DM, Norton R, Warren S, McEwan B, Urkude Poirot E, Price RN, Khan WA. G6PD Deficiency and Antimalarial R, Murthy S, Robertson T, McCallum N, Parsonson F, Bradbury RS, Efficacy for Uncomplicated Malaria in Bangladesh: A Prospective Gasser RB. More parasitic myositis cases in humans in Australia, Observational Study. PLoS One. 2016 Apr 29;11(4):e0154015. doi: and the definition of genetic markers for the causative agents as a 10.1371/journal.pone.0154015. eCollection 2016. basis for molecular diagnosis. Infect Genet Evol. 2016 Oct;44:69- 75. doi: 10.1016/j.meegid.2016.06.026. Epub 2016 Jun 14. Li H, O’Donoghue AJ, van der Linden WA, Xie SC, Yoo E, Foe IT, Tilley L, Craik CS, da Fonseca PC, Bogyo M. Structure- and Koepfli C, Nguitragool W, Hofmann NE, Robinson LJ,Ome-Kaius function-based design of Plasmodium-selective proteasome M, Sattabongkot J, Felger I, Mueller I. Sensitive and accurate inhibitors. Nature. 2016 Feb 11;530(7589):233-6. doi: 10.1038/ quantification of human malaria parasites using droplet digital PCR nature16936. (ddPCR) Sci Rep. 2016 Dec 16;6:39183. doi: 10.1038/srep39183. Lim L, Sayers CP, Goodman CD, McFadden GI. Targeting of a Kono M, Heincke D, Wilcke L, Wong TW, Bruns C, Herrmann Transporter to the Outer Apicoplast Membrane in the Human S, Spielmann T, Gilberger TW. Pellicle formation in the malaria Malaria Parasite Plasmodium falciparum. PLoS One. 2016 Jul parasite. J Cell Sci. 2016 Feb 15;129(4):673-80. doi: 10.1242/ 21;11(7):e0159603. doi: 10.1371/journal.pone.0159603. jcs.181230. Epub 2016 Jan 13. eCollection 2016. Erratum in: PLoS One. 2016;11(8):e0161420.

Krause A, Dingemanse J, Mathis A, Marquart L, Möhrle JJ, Lin CS, Uboldi AD, Epp C, Bujard H, Tsuboi T, Czabotar PE, McCarthy JS. Pharmacokinetic/pharmacodynamic modelling Cowman AF. Multiple Plasmodium falciparum Merozoite Surface of the antimalarial effect of Actelion-451840 in an induced blood Protein 1 Complexes Mediate Merozoite Binding to Human stage malaria study in healthy subjects. Br J Clin Pharmacol. 2016 Erythrocytes. J Biol Chem. 2016 Apr 1;291(14):7703-15. doi: Aug;82(2):412-21. doi: 10.1111/bcp.12962. Epub 2016 May 11. 10.1074/jbc.M115.698282. Epub 2016 Jan 28.

Krishnarjuna B, Andrew D, MacRaild CA, Morales RA, Beeson JG, Linterman MA, Hill DL. Can follicular helper T cells be targeted Anders RF, Richards JS, Norton RS. Strain-transcending immune to improve vaccine efficacy? F1000Res. 2016 Jan 20;5. pii: F1000 response generated by chimeras of the malaria vaccine candidate Faculty Rev-88. doi: 10.12688/f1000research.7388.1. eCollection merozoite surface protein 2. Sci Rep. 2016 Feb 11;6:20613. doi: 2016. Review. 10.1038/srep20613. Littler DR, Bullen HE, Harvey KL, Beddoe T, Crabb BS, Rossjohn Le Bihan A, Wirjanata G, Marfurt J et al Characterization of Novel J, Gilson PR. Disrupting the Allosteric Interaction between Antimalarial Compound ACT-451840: Preclinical Assessment the Plasmodium falciparum cAMP-dependent Kinase and Its

Page 64 Appendix 1

Regulatory Subunit. J Biol Chem. 2016 Dec 2;291(49):25375- Throughput Assay for Screening and Profiling Transmission- 25386. Epub 2016 Oct 13. Blocking Compounds against Plasmodium falciparum Gametocytes. Antimicrob Agents Chemother. 2016 Mar Liu Y, Griffin JB, Muehlenbachs A,Rogerson SJ, Bailis AJ, Sharma 25;60(4):2097-107. doi: 10.1128/AAC.01949-15. Print 2016 Apr. R, Sullivan DJ, Tshefu AK, Landis SH, Kabongo JM, Taylor SM, Meshnick SR. Diagnosis of placental malaria in poorly fixed and Mackinnon MJ, Ndila C, Uyoga S, Macharia A, Snow RW, Band processed placental tissue. Malar J. 2016 May 10;15(1):272. doi: G, Rautanen A, Rockett KA, Kwiatkowski DP, Williams TN. 10.1186/s12936-016-1314-6. Environmental Correlation Analysis for Genes Associated with Protection against Malaria. Mol Biol Evol. 2016 May;33(5):1188- Llewellyn S, Inpankaew T, Nery SV, Gray DJ, Verweij JJ, Clements 204. doi: 10.1093/molbev/msw004. Epub 2016 Jan 6. AC, Gomes SJ, Traub R, McCarthy JS. Application of a Multiplex Quantitative PCR to Assess Prevalence and Intensity Of Intestinal MacRaild CA, Richards JS, Anders RF, Norton RS. Antibody Parasite Infections in a Controlled Clinical Trial. PLoS Negl Trop Dis. Recognition of Disordered Antigens. Structure. 2016 Jan 2016 Jan 28;10(1):e0004380. doi: 10.1371/journal.pntd.0004380. 5;24(1):148-57. doi: 10.1016/j.str.2015.10.028. Epub 2015 Dec eCollection 2016 Jan. 17.

Longley RJ, Sattabongkot J, Mueller I. Insights into the Marasini N, Giddam AK, Khalil ZG, Hussein WM, Capon RJ, Batzloff naturally acquired immune response to Plasmodium vivax MR, Good MF, Toth I, Skwarczynski M. Double adjuvanting malaria. Parasitology. 2016 Feb;143(2):154-70. doi: 10.1017/ strategy for peptide-based vaccines: trimethyl chitosan S0031182015000670. Review. nanoparticles for lipopeptide delivery. Nanomedicine (Lond). 2016 Dec;11(24):3223-3235. Epub 2016 Nov 10. Longley RJ, Sripoorote P, Chobson P, Saeseu T, Sukasem C, Phuanukoonnon S, Nguitragool W, Mueller I, Sattabongkot J. High McCarthy JS, Baker M, O’Rourke P, Marquart L, Griffin P, Hooft van Efficacy of Primaquine Treatment for Plasmodium vivax in Western Huijsduijnen R, Möhrle JJ. Efficacy of OZ439 (artefenomel) against Thailand. Am J Trop Med Hyg. 2016 Nov 2;95(5):1086-1089. Epub early Plasmodium falciparum blood-stage malaria infection in 2016 Sep 6. healthy volunteers. J Antimicrob Chemother. 2016 Sep;71(9):2620- 7. doi: 10.1093/jac/dkw174. Epub 2016 Jun 5. Loughland JR, Minigo G, Burel J, Tipping PE, Piera KA, Amante FH, Engwerda CR, Good MF, Doolan DL, Anstey NM, McCarthy McCarthy JS, Marquart L, Sekuloski S, Trenholme K, Elliott S, JS, Woodberry T. Profoundly Reduced CD1c+ Myeloid Dendritic Griffin P, Rockett R, O’Rourke P, Sloots T, Angulo-Barturen I, Ferrer Cell HLA-DR and CD86 Expression and Increased Tumor Necrosis S, Jiménez-Díaz MB, Martínez MS, Hooft van Huijsduijnen R, Factor Production in Experimental Human Blood-Stage Malaria Duparc S, Leroy D, Wells TN, Baker M, Möhrle JJ. Linking Murine Infection. Infect Immun. 2016 Apr 22;84(5):1403-12. doi: 10.1128/ and Human Plasmodium falciparum Challenge Models in a IAI.01522-15. Print 2016 May. Translational Path for Antimalarial Drug Development. Antimicrob Agents Chemother. 2016 May 23;60(6):3669-75. doi: 10.1128/ Loukas A, Hotez PJ, Diemert D, Yazdanbakhsh M, McCarthy JS, AAC.02883-15. Print 2016 Jun. Correa-Oliveira R, Croese J, Bethony JM Hookworm infection. Nat Rev Dis Primers. 2016 Dec 8;2:16088. doi: 10.1038/nrdp.2016.88. McCarthy JS, Rückle T, Djeriou E, Cantalloube C, Ter-Minassian D, Review. Baker M, O’Rourke P, Griffin P, Marquart L, Hooft van Huijsduijnen R, Möhrle JJ. A Phase II pilot trial to evaluate safety and efficacy of Lucantoni L, Fidock DA, Avery VM. Luciferase-Based, High- ferroquine against early Plasmodium falciparum in an induced

Page 65 Appendix 1

blood-stage malaria infection study. Malar J. 2016 Sep 13;15:469. Ortiz L, Sanz S, Piqueras M, Desai M, Mayor A, Del Portillo H, doi: 10.1186/s12936-016-1511-3. Menéndez C, Severini C Microsatellite Genotyping of Plasmodium vivax Isolates from Pregnant Women in Four Malaria Endemic McConville MJ. Metabolic Crosstalk between Leishmania and the Countries. PLoS One. 2016 Mar 24;11(3):e0152447. doi: 10.1371/ Macrophage Host. Trends Parasitol. 2016 Sep;32(9):666-8. doi: journal.pone.0152447. eCollection 2016. 10.1016/j.pt.2016.05.005. Epub 2016 May 24. Mitcheson DF, Bottrill AR, Carr K, Coxon CR, Cano C, Golding BT, McLean AR, Boel ME, McGready R, Ataide R, Drew D, Tsuboi T, Griffin RJ, Fry AM,Doerig C, Bayliss R, Tobin AB. A new tool for Beeson JG, Nosten F, Simpson JA, Fowkes FJ. Antibody responses the chemical genetic investigation of the Plasmodium falciparum to Plasmodium falciparum and Plasmodium vivax blood-stage and Pfnek-2 NIMA-related kinase. Malar J. 2016 Nov 7;15(1):535. sporozoite antigens in the postpartum period. Sci Rep. 2016 Aug 25;6:32159. doi: 10.1038/srep32159. Mofiz E, Seemann T, Bahlo M,Holt D, Currie BJ, Fischer K, Papenfuss AT. Mitochondrial Genome Sequence of the McNamara HA, Cockburn IA. The three Rs: Recruitment, Scabies Mite Provides Insight into the Genetic Diversity of Retention and Residence of leukocytes in the liver. Clin Transl Individual Scabies Infections. PLoS Negl Trop Dis. 2016 Feb Immunology. 2016 Dec 30;5(12):e123. doi: 10.1038/cti.2016.84. 12;10(2):e0004384. doi: 10.1371/journal.pntd.0004384. eCollection 2016 Dec. Review. eCollection 2016 Feb.

Meister I, Kovac J, Duthaler U, Odermatt P, Huwyler J, Molehin AJ, Rojo JU, Siddiqui SZ, Gray SA, Carter D, Siddiqui AA. Vanobberghen F, Sayasone S, Keiser J. Pharmacokinetic Development of a schistosomiasis vaccine. Expert Rev Vaccines. Study of Praziquantel Enantiomers and Its Main Metabolite 2016 May;15(5):619-27. doi: 10.1586/14760584.2016.1131127. R-trans-4-OH-PZQ in Plasma, Blood and Dried Blood Spots in Epub 2016 Jan 13. Review. Opisthorchis viverrini-Infected Patients. PLoS Negl Trop Dis. 2016 May 6;10(5):e0004700. doi: 10.1371/journal.pntd.0004700. Montes de Oca M, Good MF, McCarthy JS, Engwerda CR. The eCollection 2016 May. Impact of Established Immunoregulatory Networks on Vaccine Efficacy and the Development of Immunity to Malaria. J Immunol. Meister I, Leonidova A, Kovač J, Duthaler U, Keiser J, Huwyler J. 2016 Dec 15;197(12):4518-4526. Review. Development and validation of an enantioselective LC-MS/MS method for the analysis of the anthelmintic drug praziquantel Montes de Oca M, Kumar R, Rivera FL, Amante FH, Sheel M, Faleiro and its main metabolite in human plasma, blood and dried blood RJ, Bunn PT, Best SE, Beattie L, Ng SS, Edwards CL, Boyle GM, spots. J Pharm Biomed Anal. 2016 Jan 25;118:81-8. doi: 10.1016/j. Price RN, Anstey NM, Loughland JR, Burel J, Doolan DL, Haque A, jpba.2015.10.011. Epub 2015 Oct 22. McCarthy JS, Engwerda CR. Type I Interferons Regulate Immune Responses in Humans with Blood-Stage Plasmodium falciparum Ménard D, Barnadas C, Fola AA, Sutherland C et al A Worldwide Infection. Cell Rep. 2016 Oct 4;17(2):399-412. doi: 10.1016/j. Map of Plasmodium falciparum K13-Propeller Polymorphisms. celrep.2016.09.015. N Engl J Med. 2016 Jun 23;374(25):2453-64. doi: 10.1056/ NEJMoa1513137. Moore BR, Benjamin JM, Auyeung SO, Salman S, Yadi G, Griffin S, Page-Sharp M, Batty KT, Siba PM, Mueller I, Rogerson SJ, Menegon M, Bardají A, Martínez-Espinosa F, Bôtto-Menezes C, Davis TM. Safety, tolerability and pharmacokinetic properties of Ome-Kaius M, Mueller I, Betuela I, Arévalo-Herrera M, Kochar coadministered azithromycin and piperaquine in pregnant Papua S, Kochar SK, Jaju P, Hans D, Chitnis C, Padilla N, Castellanos ME, New Guinean women. Br J Clin Pharmacol. 2016 Jul;82(1):199-212.

Page 66 Appendix 1

doi: 10.1111/bcp.12910. Epub 2016 Mar 27. 2016 Oct 26;8(362):362ra143.

Moore KA, Simpson JA, Paw MK, Pimanpanarak M, Nkouawa A, Sako Y, Okamoto M, Ito A. Simple Identification of Wiladphaingern J, Rijken MJ, Jittamala P, White NJ, Fowkes FJ, Human Taenia Species by Multiplex Loop-Mediated Isothermal Nosten F, McGready R. Safety of artemisinins in first trimester Amplification in Combination with Dot Enzyme-Linked of prospectively followed pregnancies: an observational study. Immunosorbent Assay. Am J Trop Med Hyg. 2016 Jun 1;94(6):1318- Lancet Infect Dis. 2016 May;16(5):576-83. doi: 10.1016/S1473- 23. doi: 10.4269/ajtmh.15-0829. Epub 2016 Apr 4. 3099(15)00547-2. Epub 2016 Feb 8. Norcross NR, Baragaña B, Wilson C, Hallyburton I, Osuna-Cabello Morin-Adeline V, Šlapeta J. The past, present and future of M, Norval S, Riley J, Stojanovski L, Simeons FR, Porzelle A, Grimaldi fluorescent protein tags in anaerobic protozoan parasites. R, Wittlin S, Duffy S,Avery VM, Meister S, Sanz L, Jiménez-Díaz Parasitology. 2016 Mar;143(3):260-75. doi: 10.1017/ B, Angulo-Barturen I, Ferrer S, Martínez MS, Gamo FJ, Frearson S0031182015001663. Epub 2015 Dec 14. Review. JA, Gray DW, Fairlamb AH, Winzeler EA, Waterson D, Campbell SF, Willis P, Read KD, Gilbert IH. Trisubstituted Pyrimidines as Morse D, Webster W, Kalanon M, Langsley G, McFadden GI. Efficacious and Fast-Acting Antimalarials. J Med Chem. 2016 Jul Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. 14;59(13):6101-20. doi: 10.1021/acs.jmedchem.6b00028. Epub PLoS One. 2016 Jun 27;11(6):e0158174. doi: 10.1371/journal. 2016 Jun 17. pone.0158174. eCollection 2016. Oguike MC, Falade CO, Shu E, Enato IG, Watila I, Baba ES, Bruce Mounsey KE, Bernigaud C, Chosidow O, McCarthy JS. Prospects J, Webster J, Hamade P, Meek S, Chandramohan D, Sutherland for Moxidectin as a New Oral Treatment for Human Scabies. PLoS CJ, Warhurst D, Roper C. Molecular determinants of sulfadoxine- Negl Trop Dis. 2016 Mar 17;10(3):e0004389. doi: 10.1371/journal. pyrimethamine resistance in Plasmodium falciparum in Nigeria pntd.0004389. eCollection 2016 Mar and the regional emergence of dhps 431V. Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):220-229. doi: 10.1016/j. Muwanguzi J, Henriques G, Sawa P, Bousema T, Sutherland ijpddr.2016.08.004. Epub 2016 Sep 29. CJ, Beshir KB. Lack of K13 mutations in Plasmodium falciparum persisting after artemisinin combination therapy treatment of Olliaro PL, Barnwell JW, Barry A, Mendis K, Mueller I, Reeder Kenyan children. Malar J. 2016 Jan 22;15:36. doi: 10.1186/s12936- JC, Shanks GD, Snounou G, Wongsrichanalai C. Implications of 016-1095-y. Plasmodium vivax Biology for Control, Elimination, and Research. Am J Trop Med Hyg. 2016 Dec 28;95(6 Suppl):4-14. doi: 10.4269/ Nagel R, Traub RJ, Allcock RJ, Kwan MM, Bielefeldt-Ohmann ajtmh.16-0160. Epub 2016 Oct 31. H. Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients. Olveda DU, McManus DP, Ross AG. Mass drug administration and Microbiome. 2016 Aug 31;4(1):47. doi: 10.1186/s40168-016- the global control of schistosomiasis: successes, limitations and 0191-0. clinical outcomes. Curr Opin Infect Dis. 2016 Dec;29(6):595-608.

Navarro S, Pickering DA, Ferreira IB, Jones L, Ryan S, Troy S, Park JW, Cheong HK, Honda Y, Ha M, Kim H, Kolam J, Inape K, Leech A, Hotez PJ, Zhan B, Laha T, Prentice R, Sparwasser T, Mueller I. Time trend of malaria in relation to climate variability Croese J, Engwerda CR, Upham JW, Julia V, Giacomin PR, Loukas in Papua New Guinea. Environ Health Toxicol. 2016 Feb A. Hookworm recombinant protein promotes regulatory T cell 25;31:e2016003. doi: 10.5620/eht.e2016003. eCollection 2016. responses that suppress experimental asthma. Sci Transl Med.

Page 67 Appendix 1

Pasaje CF, Cheung V, Kennedy K, Lim EE, Baell JB, Griffin MD, 23;187:341-52. doi: 10.1039/c5fd00181a. Ralph SA. Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum. Sci Petersen W, Külzer S, Engels S, Zhang Q, Ingmundson A, Rug M, Rep. 2016 Jun 9;6:27531. doi: 10.1038/srep27531. Maier AG, Przyborski JM. J-dot targeting of an exported HSP40 in Plasmodium falciparum-infected erythrocytes. Int J Parasitol. 2016 Pasay CJ, Rockett R, Sekuloski S, Griffin P, Marquart L,Peatey Jul;46(8):519-25. doi: 10.1016/j.ijpara.2016.03.005. Epub 2016 C, Wang CY, O’Rourke P, Elliott S, Baker M, Möhrle JJ, McCarthy Apr 7. JS. Piperaquine Monotherapy of Drug-Susceptible Plasmodium falciparum Infection Results in Rapid Clearance of Parasitemia but Phillips MA, Wirjanata G, Marfurt J et al A Triazolopyrimidine- Is Followed by the Appearance of Gametocytemia. J Infect Dis. Based Dihydroorotate Dehydrogenase Inhibitor with Improved 2016 Jul 1;214(1):105-13. doi: 10.1093/infdis/jiw128. Epub 2016 Drug-like Properties for Treatment and Prevention of Malaria. ACS Apr 7. Infect Dis. 2016 Dec 9;2(12):945-957. Epub 2016 Oct 4.

Pava Z, Burdam FH, Handayuni I, Trianty L, Utami RA, Tirta Pilotte N, Papaiakovou M, Grant JR, Bierwert LA, Llewellyn S, YK, Kenangalem E, Lampah D, Kusuma A, Wirjanata G, Kho McCarthy JS, Williams SA. Improved PCR-Based Detection of S, Simpson JA, Auburn S, Douglas NM, Noviyanti R, Anstey Soil Transmitted Helminth Infections Using a Next-Generation NM, Poespoprodjo JR, Marfurt J, Price RN. Submicroscopic Sequencing Approach to Assay Design. PLoS Negl Trop Dis. 2016 and Asymptomatic Plasmodium Parasitaemia Associated with Mar 30;10(3):e0004578. doi: 10.1371/journal.pntd.0004578. Significant Risk of Anaemia in Papua, Indonesia. PLoS One. 2016 eCollection 2016 Mar. Oct 27;11(10):e0165340. doi: 10.1371/journal.pone.0165340. eCollection 2016. Prinz B, Harvey KL, Wilcke L, Ruch U, Engelberg K, Biller L, Lucet I, Erkelenz S, Heincke D, Spielmann T, Doerig C, Kunick C, Crabb Payne RO, McCarthy JS et al Demonstration of the Blood-Stage BS, Gilson PR, Gilberger TW. Hierarchical phosphorylation of apical Plasmodium falciparum Controlled Human Malaria Infection Model membrane antigen 1 is required for efficient red blood cell invasion to Assess Efficacy of the P. falciparum Apical Membrane Antigen 1 by malaria parasites. Sci Rep. 2016 Oct 4;6:34479. doi: 10.1038/ Vaccine, FMP2.1/AS01. J Infect Dis. 2016 Jun 1;213(11):1743-51. srep34479. doi: 10.1093/infdis/jiw039. Epub 2016 Feb 4. Erratum in: J Infect Dis. 2016 Sep 15;214(6):978. Proellocks NI, Coppel RL, Mohandas N, Cooke BM. Malaria Parasite Proteins and Their Role in Alteration of the Structure and Pearson MS, Loukas A. The parasite’s new clothes. Elife. 2016 Apr Function of Red Blood Cells. Adv Parasitol. 2016;91:1-86. doi: 15;5:e15957. doi: 10.7554/eLife.15957. 10.1016/bs.apar.2015.09.002. Epub 2015 Dec 14. Review

Pearson RD, Auburn S, Marfurt J, Harrison G, Barry A, Mueller Proietti C, Zakrzewski M, Watkins TS, Berger B, Hasan S, I et al Genomic analysis of local variation and recent evolution Ratnatunga CN, Brion MJ, Crompton PD, Miles JJ, Doolan DL, in Plasmodium vivax. Nat Genet. 2016 Aug;48(8):959-64. doi: Krause L. Mining, visualizing and comparing multidimensional 10.1038/ng.3599. Epub 2016 Jun 27. biomolecular data using the Genomics Data Miner (GMine) Web- Server. Sci Rep. 2016 Dec 6;6:38178. doi: 10.1038/srep38178. Perez-Guaita D, Andrew D, Heraud P, Beeson J, Anderson D, Richards J, Wood BR. High resolution FTIR imaging provides Pulford J, Smith I, Mueller I, Siba PM, Hetzel MW. Health automated discrimination and detection of single malaria Worker Compliance with a ‘Test And Treat’ Malaria Case parasite infected erythrocytes on glass. Faraday Discuss. 2016 Jun Management Protocol in Papua New Guinea. PLoS One. 2016

Page 68 Appendix 1

Jul 8;11(7):e0158780. doi: 10.1371/journal.pone.0158780. Requena P, Rui E, Padilla N, Martínez-Espinosa FE, Castellanos eCollection 2016. ME, Bôtto-Menezes C, Malheiro A, Arévalo-Herrera M, Kochar S, Kochar SK, Kochar DK, Umbers AJ, Ome-Kaius M, Wangnapi R, Quadt KA, Streichfuss M, Moreau CA, Spatz JP, Frischknecht F. Hans D, Menegon M, Mateo F, Sanz S, Desai M, Mayor A, Chitnis Coupling of Retrograde Flow to Force Production During Malaria CC, Bardají A, Mueller I, Rogerson S, Severini C, Fernández- Parasite Migration. ACS Nano. 2016 Feb 23;10(2):2091-102. doi: Becerra C, Menéndez C, Del Portillo H, Dobaño C. Plasmodium 10.1021/acsnano.5b06417. Epub 2016 Jan 27. vivax VIR Proteins Are Targets of Naturally-Acquired Antibody and T Cell Immune Responses to Malaria in Pregnant Women. PLoS Raja AI, Cai Y, Reiman JM, Groves P, Chakravarty S, McPhun V, Negl Trop Dis. 2016 Oct 6;10(10):e0005009. doi: 10.1371/journal. Doolan DL, Cockburn I, Hoffman SL, Stanisic DI,Good MF. pntd.0005009. eCollection 2016 Oct. Chemically Attenuated Blood-Stage Plasmodium yoelii Parasites Induce Long-Lived and Strain-Transcending Protection. Infect Reynaldi A, Schlub TE, Chelimo K, Sumba PO, Piriou E, Ogolla Immun. 2016 Jul 21;84(8):2274-88. doi: 10.1128/IAI.00157-16. S, Moormann AM, Rochford R, Davenport MP. Impact of Print 2016 Aug. Plasmodium falciparum Coinfection on Longitudinal Epstein- Barr Virus Kinetics in Kenyan Children. J Infect Dis. 2016 Mar Rajahram GS, Barber BE, William T, Grigg MJ, Menon J, Yeo TW, 15;213(6):985-91. doi: 10.1093/infdis/jiv525. Epub 2015 Nov 3. Anstey NM. Falling Plasmodium knowlesi Malaria Death Rate among Adults despite Rising Incidence, Sabah, Malaysia, 2010- Reynaldi A, Schlub TE, Piriou E, Ogolla S, Sumba OP, Moormann 2014. Emerg Infect Dis. 2016 Jan;22(1):41-8. doi: 10.3201/ AM, Rochford R, Davenport MP. Modeling of EBV Infection and eid2201.151305. Antibody Responses in Kenyan Infants With Different Levels of Malaria Exposure Shows Maternal Antibody Decay is a Major Rashwan N, Bourguinat C, Keller K, Gunawardena NK, de Silva N, Determinant of Early EBV Infection. J Infect Dis. 2016 Nov Prichard R. Isothermal Diagnostic Assays for Monitoring Single 1;214(9):1390-1398. Epub 2016 Aug 28. Nucleotide Polymorphisms in Necator americanus Associated with Benzimidazole Drug Resistance. PLoS Negl Trop Dis. 2016 Rhiel M, Bittl V, Tribensky A, Charnaud SC, Strecker M, Müller S, Dec 8;10(12):e0005113. doi: 10.1371/journal.pntd.0005113. Lanzer M, Sanchez C, Schaeffer-Reiss C, Westermann B,Crabb eCollection 2016 Dec. BS, Gilson PR, Külzer S, Przyborski JM. Trafficking of the exported P. falciparum chaperone PfHsp70x. Sci Rep. 2016 Nov 8;6:36174. Ravenhall M, Benavente ED, Mipando M, Jensen AT, Sutherland doi: 10.1038/srep36174. CJ, Roper C, Sepúlveda N, Kwiatkowski DP, Montgomery J, Phiri KS, Terlouw A, Craig A, Campino S, Ocholla H, Clark TG. Characterizing Ricciardi A, Visitsunthorn K, Dalton JP, Ndao M. A vaccine the impact of sustained sulfadoxine/pyrimethamine use upon the consisting of Schistosoma mansoni cathepsin B formulated in Plasmodium falciparum population in Malawi. Malar J. 2016 Nov Montanide ISA 720 VG induces high level protection against 29;15(1):575. murine schistosomiasis. BMC Infect Dis. 2016 Mar 5;16:112. doi: 10.1186/s12879-016-1444-z Reimer LJ, Thomsen EK, Koimbu G, Keven JB, Mueller I, Siba PM, Kazura JW, Hetzel MW, Zimmerman PA. Malaria transmission Richards SN, Nash MN, Baker ES, Webster MW, Lehane dynamics surrounding the first nationwide long-lasting insecticidal AM, Shafik SH, Martin RE. Molecular Mechanisms for Drug net distribution in Papua New Guinea. Malar J. 2016 Jan 12;15:25. Hypersensitivity Induced by the Malaria Parasite’s Chloroquine doi: 10.1186/s12936-015-1067-7. Resistance Transporter. PLoS Pathog. 2016 Jul 21;12(7):e1005725. doi: 10.1371/journal.ppat.1005725. eCollection 2016 Jul.

Page 69 Appendix 1

Riglar DT, Whitehead L, Cowman AF, Rogers KL, Baum J. Satyagraha AW, Sadhewa A, Elvira R, Elyazar I, Feriandika D, Localisation-based imaging of malarial antigens during erythrocyte Antonjaya U, Oyong D, Subekti D, Rozi IE, Domingo GJ, Harahap entry reaffirms a role for AMA1 but not MTRAP in invasion. J Cell AR, Baird JK. Assessment of Point-of-Care Diagnostics for G6PD Sci. 2016 Jan 1;129(1):228-42. doi: 10.1242/jcs.177741. Epub Deficiency in Malaria Endemic Rural Eastern Indonesia. PLoS Negl 2015 Nov 24. Trop Dis. 2016 Feb 19;10(2):e0004457. doi: 10.1371/journal. pntd.0004457. eCollection 2016 Feb. Rodriguez-Barraquer I, Arinaitwe E, Jagannathan P, Boyle MJ, Tappero J, Muhindo M, Kamya MR, Dorsey G, Drakeley Scott FJ, Khalaf AI, Duffy S,Avery VM, Suckling CJ. Selective anti- C, Ssewanyana I, Smith DL, Greenhouse B. Quantifying malarial minor groove binders. Bioorg Med Chem Lett. 2016 Jul Heterogeneous Malaria Exposure and Clinical Protection in a 15;26(14):3326-9. doi: 10.1016/j.bmcl.2016.05.039. Epub 2016 Cohort of Ugandan Children. J Infect Dis. 2016 Oct 1;214(7):1072- May 13 80. doi: 10.1093/infdis/jiw301. Epub 2016 Aug 1. Scott FJ, Khalaf AI, Giordani F, Wong PE, Duffy S, Barrett M,Avery Ross A, Koepfli C, Schoepflin S, Timinao L, Siba P, Smith T,Mueller VM, Suckling CJ. An evaluation of Minor Groove Binders as anti- I, Felger I, Tanner M. The Incidence and Differential Seasonal Trypanosoma brucei brucei therapeutics. Eur J Med Chem. 2016 Patterns of Plasmodium vivax Primary Infections and Relapses in Jun 30;116:116-25. doi: 10.1016/j.ejmech.2016.03.064. Epub a Cohort of Children in Papua New Guinea. PLoS Negl Trop Dis. 2016 Mar 29. 2016 May 4;10(5):e0004582. doi: 10.1371/journal.pntd.0004582. eCollection 2016 May. Sebina I, James KR, Soon MS, Fogg LG, Best SE, Labastida Rivera F, Montes de Oca M, Amante FH, Thomas BS, Beattie L, Souza- Russell S, Rahmani R, Jones AJ, Newson HL, Neilde K, Cotillo I, Fonseca-Guimaraes F, Smyth MJ, Hertzog PJ, Hill GR, Hutloff A, Rahmani Khajouei M, Ferrins L, Qureishi S, Nguyen N, Martinez- Engwerda CR, Haque A. IFNAR1-Signalling Obstructs ICOS- Martinez MS, Weaver DF, Kaiser M, Riley J, Thomas J, De Rycker mediated Humoral Immunity during Non-lethal Blood-Stage M, Read KD, Flematti GR, Ryan E, Tanghe S, Rodriguez A, Plasmodium Infection. PLoS Pathog. 2016 Nov 3;12(11):e1005999. Charman SA, Kessler A, Avery VM, Baell JB, Piggott MJ. Hit-to- doi: 10.1371/journal.ppat.1005999. eCollection 2016 Nov. Lead Optimization of a Novel Class of Potent, Broad-Spectrum Trypanosomacides. J Med Chem. 2016 Nov 10;59(21):9686-9720. Sedaghat B, Stephenson RJ, Giddam AK, Eskandari S, Apte SH, Epub 2016 Sep 19. Pattinson DJ, Doolan DL, Toth I. Synthesis of Mannosylated Lipopeptides with Receptor Targeting Properties. Bioconjug Ryg-Cornejo V, Ioannidis LJ, Ly A, Chiu CY, Tellier J, Hill DL, Chem. 2016 Mar 16;27(3):533-48. doi: 10.1021/acs. Preston SP, Pellegrini M, Yu D, Nutt SL, Kallies A, Hansen DS bioconjchem.5b00547. Epub 2016 Jan 25. Severe Malaria Infections Impair Germinal Center Responses by Inhibiting T Follicular Helper Cell Differentiation. Cell Rep. 2016 Sercundes MK, Ortolan LS, Debone D, Soeiro-Pereira PV, Jan 5;14(1):68-81. doi: 10.1016/j.celrep.2015.12.006. Epub 2015 Gomes E, Aitken EH, Neto AC, Russo M, D’ Império Lima MR, Dec 24. Alvarez JM, Portugal S, Marinho CR, Epiphanio S Targeting Neutrophils to Prevent Malaria-Associated Acute Lung Injury/ Ryg-Cornejo V, Ly A, Hansen DS. Immunological processes Acute Respiratory Distress Syndrome in Mice. PLoS Pathog. 2016 underlying the slow acquisition of humoral immunity to Dec 7;12(12):e1006054. doi: 10.1371/journal.ppat.1006054. malaria. Parasitology. 2016 Feb;143(2):199-207. doi: 10.1017/ eCollection 2016 Dec. S0031182015001705. Epub 2016 Jan 8. Review. Shearer FM, Huang Z, Weiss DJ, Wiebe A, Gibson HS, Battle

Page 70 Appendix 1

KE, Pigott DM, Brady OJ, Putaporntip C, Jongwutiwes S, Lau YL, Srivastava A, Philip N, Hughes KR, Georgiou K, MacRae JI, Barrett Manske M, Amato R, Elyazar IR, Vythilingam I, Bhatt S, Gething PW, MP, Creek DJ, McConville MJ, Waters AP. Stage-Specific Changes Singh B, Golding N, Hay SI, Moyes CL. Estimating Geographical in Plasmodium Metabolism Required for Differentiation and Variation in the Risk of Zoonotic Plasmodium knowlesi Infection Adaptation to Different Host and Vector Environments. PLoS in Countries Eliminating Malaria. PLoS Negl Trop Dis. 2016 Pathog. 2016 Dec 27;12(12):e1006094. doi: 10.1371/journal. Aug 5;10(8):e0004915. doi: 10.1371/journal.pntd.0004915. ppat.1006094. eCollection 2016 Dec. eCollection 2016 Aug. Stanisic DI, Gerrard J, Fink J, Griffin PM, Liu XQ, Sundac L, Sekuloski Silberhorn E, Schwartz U, Löffler P, Schmitz S, Symelka A, de S, Rodriguez IB, Pingnet J, Yang Y, Zhou Y, Trenholme KR, Wang Koning-Ward T, Merkl R, Längst G. Plasmodium falciparum CY, Hackett H, Chan JA, Langer C, Hanssen E, Hoffman SL,Beeson Nucleosomes Exhibit Reduced Stability and Lost Sequence JG, McCarthy JS, Good MF. Infectivity of Plasmodium falciparum Dependent Nucleosome Positioning. PLoS Pathog. 2016 Dec in Malaria-Naive Individuals Is Related to Knob Expression 29;12(12):e1006080. doi: 10.1371/journal.ppat.1006080. and Cytoadherence of the Parasite. Infect Immun. 2016 Aug eCollection 2016 Dec. 19;84(9):2689-96. doi: 10.1128/IAI.00414-16. Print 2016 Sep.

Skinner-Adams TS, Sumanadasa SD, Fisher GM, Davis RA, Stanisic DI, Good MF. Examining cellular immune responses to Doolan DL, Andrews KT. Defining the targets of antiparasitic inform development of a blood-stage malaria vaccine. Parasitology. compounds. Drug Discov Today. 2016 May;21(5):725-39. doi: 2016 Feb;143(2):208-23. doi: 10.1017/S0031182015001092. 10.1016/j.drudis.2016.01.002. Epub 2016 Jan 16. Review. Epub 2016 Jan 8. Review.

Smith D, Tikhonova IG, Jewhurst HL, Drysdale OC, Dvořák J, Stark D, Barratt J, Chan D, Ellis JT. Dientamoeba fragilis, the Robinson MW, Cwiklinski K, Dalton JP. Unexpected Activity of a Neglected Trichomonad of the Human Bowel. Clin Microbiol Rev. Novel Kunitz-type Inhibitor: Inhibition of Cysteine Proteases But 2016 Jul;29(3):553-80. doi: 10.1128/CMR.00076-15. Review. Not Serine Proteases. J Biol Chem. 2016 Sep 9;291(37):19220-34. doi: 10.1074/jbc.M116.724344. Epub 2016 Jul 15. Stephenson RJ, Toth I, Liang J, Mangat A, McManus DP, You H. Identification of Host Insulin Binding Sites on Sotillo J, Pearson M, Potriquet J, Becker L, Pickering D, Mulvenna Schistosoma japonicum Insulin Receptors. PLoS One. 2016 J, Loukas A. Extracellular vesicles secreted by Schistosoma Jul 21;11(7):e0159704. doi: 10.1371/journal.pone.0159704. mansoni contain protein vaccine candidates. Int J Parasitol. 2016 eCollection 2016. Jan;46(1):1-5. doi: 10.1016/j.ijpara.2015.09.002. Epub 2015 Oct 13. Stewart RJ, Ferguson DJ, Whitehead L, Bradin CH, Wu HJ, Tonkin CJ. Phosphorylation of αSNAP is Required for Secretory Organelle Spillman NJ, Dalmia VK, Goldberg DE. Exported Epoxide Biogenesis in Toxoplasma gondii. Traffic. 2016 Feb;17(2):102-16. Hydrolases Modulate Erythrocyte Vasoactive Lipids during doi: 10.1111/tra.12348. Epub 2015 Dec 14. Plasmodium falciparum Infection. MBio. 2016 Oct 18;7(5). pii: e01538-16. doi: 10.1128/mBio.01538-16. Stoessel D, Nowell, CJ, Jones, AJ, Ferrins, L, Ellis KM, Riley J, Rahmani R, Read KD, McConville MJ, Avery VM, Baell JB, Srivastava A, Kowalski GM, Callahan DL, Meikle PJ, Creek DJ. Creek, DJ. Metabolomics and lipidomics reveal perturbation Strategies for Extending Metabolomics Studies with Stable Isotope of sphingolipid metabolism by a novel anti-trypanosomal Labelling and Fluxomics. Metabolites. 2016 Oct 1;6(4). pii: E32. 3-(oxazolo[4,5-b]pyridine-2-yl)anilide. Metabolomics (2016) 12: 126. doi:10.1007/s11306-016-1062-1

Page 71 Appendix 1

Stroehlein AJ, Young ND, Hall RS, Korhonen PK, Hofmann A, Trends Parasitol. 2016 Nov;32(11):887-898. doi: 10.1016/j. Sternberg PW, Jabbar A, Gasser RB. CAP protein superfamily pt.2016.07.003. Epub 2016 Aug 18. Review. members in Toxocara canis. Parasit Vectors. 2016 Jun 24;9(1):360. doi: 10.1186/s13071-016-1642-y. Terán D, Hocková D, Česnek M, Zíková A, Naesens L, Keough DT, Guddat LW. Crystal structures and inhibition of Trypanosoma Sutherland CJ. Persistent Parasitism: The Adaptive Biology of brucei hypoxanthine-guanine phosphoribosyltransferase. Sci Rep. Malariae and Ovale Malaria. Trends Parasitol. 2016 Oct;32(10):808- 2016 Oct 27;6:35894. doi: 10.1038/srep35894. 819. doi: 10.1016/j.pt.2016.07.001. Epub 2016 Jul 30. Review. Theunissen C, Bottieau E, Van Gompel A, Siozopoulou V, Bradbury Sutton PL, Luo Z, Divis PC, Friedrich VK, Conway DJ, Singh RS. Presumptive Gnathostoma binucleatum-infection in a Belgian B, Barnwell JW, Carlton JM, Sullivan SA Characterizing the traveler returning from South America Travel Med Infect Dis. 2016 genetic diversity of the monkey malaria parasite Plasmodium Mar-Apr;14(2):170-1. doi: 10.1016/j.tmaid.2016.02.003. Epub cynomolgi. Infect Genet Evol. 2016 Jun;40:243-52. doi: 10.1016/j. 2016 Mar 4 meegid.2016.03.009. Epub 2016 Mar 14. Thomas J, Carson CF, Peterson GM, Walton SF, Hammer KA, Tamarozzi F, Covini I, Mariconti M, Narra R, Tinelli C, De Silvestri A, Naunton M, Davey RC, Spelman T, Dettwiller P, Kyle G, Cooper GM, Manzoni F, Casulli A, Ito A, Neumayr A, Brunetti E. Comparison of Baby KE. Therapeutic Potential of Tea Tree Oil for Scabies. Am J Trop the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis Med Hyg. 2016 Feb;94(2):258-66. doi: 10.4269/ajtmh.14-0515. of Hepatic Cystic Echinococcosis in Humans. PLoS Negl Trop Dis. Epub 2016 Jan 19. Review. 2016 Feb 12;10(2):e0004444. doi: 10.1371/journal.pntd.0004444. eCollection 2016 Feb. Tilley L, Straimer J, Gnädig NF, Ralph SA, Fidock DA. Artemisinin Action and Resistance in Plasmodium falciparum. Trends Parasitol. Tasani M, Tong SY, Andrews RM, Holt DC, Currie BJ, Carapetis 2016 Sep;32(9):682-96. doi: 10.1016/j.pt.2016.05.010. Epub JR, Bowen AC. The Importance of Scabies Coinfection in the 2016 Jun 9. Review. Treatment Considerations for Impetigo. Pediatr Infect Dis J. 2016 Apr;35(4):374-8. doi: 10.1097/INF.0000000000001013. Tran PN, Brown SH, Rug M, Ridgway MC, Mitchell TW, Maier AG. Changes in lipid composition during sexual development of Tchaparian E, Sambol NC, Arinaitwe E, McCormack SA, Bigira the malaria parasite Plasmodium falciparum. Malar J. 2016 Feb V, Wanzira H, Muhindo M, Creek DJ, Sukumar N, Blessborn D, 6;15:73. doi: 10.1186/s12936-016-1130-z Tappero JW, Kakuru A, Bergqvist Y, Aweeka FT, Parikh S. Population Pharmacokinetics and Pharmacodynamics of Lumefantrine in Tran PN, Tate CJ, Ridgway MC, Saliba KJ, Kirk K, Maier AG. Young Ugandan Children Treated With Artemether-Lumefantrine Human dihydrofolate reductase influences the sensitivity of the for Uncomplicated Malaria. J Infect Dis. 2016 Oct 15;214(8):1243- malaria parasite Plasmodium falciparum to ketotifen - A cautionary 51. doi: 10.1093/infdis/jiw338. Epub 2016 Jul 28. tale in screening transgenic parasites. Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):179-183. doi: 10.1016/j.ijpddr.2016.09.003. Tebeje BM, Harvie M, You H, Loukas A, McManus DP. Epub 2016 Sep 22. Schistosomiasis vaccines: where do we stand? Parasit Vectors. 2016 Sep 30;9(1):528. Review. Tran TB, Cheah SE, Yu HH, Bergen PJ, Nation RL, Creek DJ, Purcell A, Forrest A, Doi Y, Song J, Velkov T, Li J. Anthelmintic closantel Teo A, Feng G, Brown GV, Beeson JG, Rogerson SJ. Functional enhances bacterial killing of polymyxin B against multidrug- Antibodies and Protection against Blood-stage Malaria. resistant Acinetobacter baumannii. J Antibiot (Tokyo). 2016

Page 72 Appendix 1

Jun;69(6):415-21. doi: 10.1038/ja.2015.127. Epub 2015 Dec 16. Van Voorhis WC, Andrews KT, Avery VM, Kirk K, Lehane AM et al Open Source Drug Discovery with the Malaria Box Compound Ujvari B, Beckmann C, Biro PA, Arnal A, Tasiemski A, Massol F, Collection for Neglected Diseases and Beyond. PLoS Pathog. Salzet M, Mery F, Boidin-Wichlacz C, Misse D, Renaud F, Vittecoq 2016 Jul 28;12(7):e1005763. doi: 10.1371/journal.ppat.1005763. M, Tissot T, Roche B, Poulin R, Thomas F. Cancer and life-history eCollection 2016 Jul. traits: lessons from host-parasite interactions. Parasitology. 2016 Apr;143(5):533-41. doi: 10.1017/S0031182016000147. Epub Veiga MI, Dhingra SK, Henrich PP, Straimer J, Gnädig N, 2016 Feb 18. Review. Uhlemann AC, Martin RE, Lehane AM, Fidock DA. Globally prevalent PfMDR1 mutations modulate Plasmodium falciparum Unger HW, Ashorn P, Cates JE, Dewey KG, Rogerson SJ. susceptibility to artemisinin-based combination therapies. Nat Undernutrition and malaria in pregnancy - a dangerous dyad? BMC Commun. 2016 May 18;7:11553. doi: 10.1038/ncomms11553. Med. 2016 Sep 19;14(1):142. doi: 10.1186/s12916-016-0695-2. Villarino NF, LeCleir GR, Denny JE, Dearth SP, Harding CL, Unger HW, Cates JE, Gutman J, Briand V, Fievet N, Valea I, Tinto H, Sloan SS, Gribble JL, Campagna SR, Wilhelm SW, Schmidt NW. d’Alessandro U, Landis SH, Adu-Afarwuah S, Dewey KG, Ter Kuile Composition of the gut microbiota modulates the severity of F, Dellicour S, Ouma P, Slutsker L, Terlouw DJ, Kariuki S, Ayisi J, malaria. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2235-40. Nahlen B, Desai M, Madanitsa M, Kalilani-Phiri L, Ashorn P, Maleta doi: 10.1073/pnas.1504887113. Epub 2016 Feb 8. K, Mueller I, Stanisic D, Schmiegelow C, Lusingu J, Westreich D, van Eijk AM, Meshnick S, Rogerson S. Maternal Malaria and Volz JC, Yap A, Sisquella X, Thompson JK, Lim NT, Whitehead LW, Malnutrition (M3) initiative, a pooled birth cohort of 13 pregnancy Chen L, Lampe M, Tham WH, Wilson D, Nebl T, Marapana D, studies in Africa and the Western Pacific. BMJ Open. 2016 Dec Triglia T, Wong W, Rogers KL, Cowman AF. Essential Role of the 21;6(12):e012697. doi: 10.1136/bmjopen-2016-012697. PfRh5/PfRipr/CyRPA Complex during Plasmodium falciparum Invasion of Erythrocytes. Cell Host Microbe. 2016 Jul 13;20(1):60- Unger HW, Wangnapi RA, Ome-Kaius M, Boeuf P, Karl S, 71. doi: 10.1016/j.chom.2016.06.004. Epub 2016 Jun 30. Mueller I, Rogerson SJ. Azithromycin-containing intermittent preventive treatment in pregnancy affects gestational weight gain, Voronin D, Bachu S, Shlossman M, Unnasch TR, Ghedin E, an important predictor of birthweight in Papua New Guinea - an Lustigman S. Glucose and Glycogen Metabolism in Brugia malayi exploratory analysis. Matern Child Nutr. 2016 Oct;12(4):699-712. Is Associated with Wolbachia Symbiont Fitness PLoS One. 2016 doi: 10.1111/mcn.12215. Epub 2015 Sep 15. Apr 14;11(4):e0153812. doi: 10.1371/journal.pone.0153812. eCollection 2016. van Dooren GG, Yeoh LM, Striepen B, McFadden GI. The Import of Proteins into the Mitochondrion of Toxoplasma gondii. Waltmann A, Karl S, Chiu C, Mueller I. Limited Degradation of the J Biol Chem. 2016 Sep 9;291(37):19335-50. doi: 10.1074/jbc. Plasmodium falciparum Gametocyte Marker pfs25 mRNA Exposed M116.725069. Epub 2016 Jul 25. to Tropical Temperatures: Considerations for Malaria Transmission Field Studies. Am J Trop Med Hyg. 2016 Apr;94(4):886-9. doi: van Schalkwyk DA, Nash MN, Shafik SH, Summers RL, Lehane 10.4269/ajtmh.15-0531. Epub 2016 Feb 8. AM, Smith PJ, Martin RE. Verapamil-Sensitive Transport of Quinacrine and Methylene Blue via the Plasmodium falciparum Wang G, Drinkwater N, Drew DR, MacRaild CA, Chalmers DK, Chloroquine Resistance Transporter Reduces the Parasite’s Mohanty B, Lim SS, Anders RF, Beeson JG, Thompson PE, Susceptibility to these Tricyclic Drugs. J Infect Dis. 2016 Mar McGowan S, Simpson JS, Norton RS, Scanlon MJ. Structure- 1;213(5):800-10. doi: 10.1093/infdis/jiv509. Epub 2015 Oct 26. Activity Studies of β-Hairpin Peptide Inhibitors of the Plasmodium

Page 73 Appendix 1

falciparum AMA1-RON2 Interaction. J Mol Biol. 2016 Oct T, Richards JS, Fowkes FJ, Beeson JG. The association between 9;428(20):3986-3998. doi: 10.1016/j.jmb.2016.07.001. Epub naturally acquired IgG subclass specific antibodies to the PfRH5 2016 Jul 14. invasion complex and protection from Plasmodium falciparum malaria. Sci Rep. 2016 Sep 8;6:33094. doi: 10.1038/srep33094. Wang H, Li J, Guo B, Zhao L, Zhang Z, McManus DP, Wen H, Zhang W. In vitro culture of Echinococcus multilocularis producing Weerakoon KG, Gordon CA, Gobert GN, Cai P, McManus DP. protoscoleces and mouse infection with the cultured vesicles. Optimisation of a droplet digital PCR assay for the diagnosis of Parasit Vectors. 2016 Jul 25;9(1):411. doi: 10.1186/s13071-016- Schistosoma japonicum infection: A duplex approach with DNA 1687-y. binding dye chemistry. J Microbiol Methods. 2016 Jun;125:19-27. doi: 10.1016/j.mimet.2016.03.012. Epub 2016 Mar 26. Wangchuk P, Giacomin PR, Pearson MS, Smout MJ, Loukas A. Identification of lead chemotherapeutic agents from medicinal Weerakoon KG, McManus DP. Cell-Free DNA as a Diagnostic plants against blood flukes and whipworms. Sci Rep. 2016 Aug Tool for Human Parasitic Infections. Trends Parasitol. 2016 30;6:32101. doi: 10.1038/srep32101. May;32(5):378-91. doi: 10.1016/j.pt.2016.01.006. Epub 2016 Feb 1. Review. Wangchuk P, Namgay K, Gayleg K, Dorji Y. Medicinal plants of Dagala region in Bhutan: their diversity, distribution, uses and Weiss GE, Crabb BS, Gilson PR. Overlaying Molecular and economic potential J Ethnobiol Ethnomed. 2016 Jun 24;12(1):28. Temporal Aspects of Malaria Parasite Invasion. Trends Parasitol. doi: 10.1186/s13002-016-0098-7. 2016 Apr;32(4):284-95. doi: 10.1016/j.pt.2015.12.007. Epub 2016 Jan 7. Review. Wangchuk P, Pearson MS, Giacomin PR, Becker L, Sotillo J, Pickering D, Smout MJ, Loukas A. Compounds Derived from the White MT, Shirreff G, Karl S, Ghani AC,Mueller I. Variation in Bhutanese Daisy, Ajania nubigena, Demonstrate Dual Anthelmintic relapse frequency and the transmission potential of Plasmodium Activity against Schistosoma mansoni and Trichuris muris. PLoS vivax malaria. Proc Biol Sci. 2016 Mar 30;283(1827):20160048. Negl Trop Dis. 2016 Aug 4;10(8):e0004908. doi: 10.1371/journal. doi: 10.1098/rspb.2016.0048. pntd.0004908. eCollection 2016 Aug. Williamson AE, Avery V, Dennis AS, Fletcher S, Kirk K, Ralph Wangchuk P, Tashi Quality assurance of the university medical SA, Todd MH et al Open Source Drug Discovery: Highly education, hospital services and traditional pharmaceutical Potent Antimalarial Compounds Derived from the Tres Cantos products of the Bhutanese So-wa-rig-pa health care system. BMC Arylpyrroles. ACS Cent Sci. 2016 Oct 26;2(10):687-701. Epub 2016 Complement Altern Med. 2016 Aug 12;16(1):283. doi: 10.1186/ Sep 14. s12906-016-1270-2. Winterberg M, Kirk K. A high-sensitivity HPLC assay for Wangchuk S, Drukpa T, Penjor K, Peldon T, Dorjey Y, Dorji K, measuring intracellular Na(+) and K(+) and its application to Chhetri V, Trimarsanto H, To S, Murphy A, von Seidlein L, Price RN, Plasmodium falciparum infected erythrocytes. Sci Rep. 2016 Jul Thriemer K, Auburn S. Where chloroquine still works: the genetic 7;6:29241. doi: 10.1038/srep29241. make-up and susceptibility of Plasmodium vivax to chloroquine plus primaquine in Bhutan. Malar J. 2016 May 12;15(1):277. doi: Wirjanata G, Handayuni I, Zaloumis SG, Chalfein F, Prayoga 10.1186/s12936-016-1320-8. P, Kenangalem E, Poespoprodjo JR, Noviyanti R, Simpson JA, Price RN, Marfurt J. Analysis of ex vivo drug response data of Weaver R, Reiling L, Feng G, Drew DR, Mueller I, Siba PM, Tsuboi Plasmodium clinical isolates: the pros and cons of different

Page 74 Appendix 1

computer programs and online platforms. Malar J. 2016 Mar Rheopathologic Consequence of Plasmodium vivax Rosette 2;15:137. doi: 10.1186/s12936-016-1173-1. Formation. PLoS Negl Trop Dis. 2016 Aug 10;10(8):e0004912. doi: 10.1371/journal.pntd.0004912. eCollection 2016 Aug. Xie SC, Dogovski C, Hanssen E, Chiu F, Yang T, Crespo MP, Stafford C,Batinovic S, Teguh S, Charman S, Klonis N, Tilley L. Zhang R, Suwanarusk R, Malleret B, Cooke BM, Nosten F, Lau Haemoglobin degradation underpins the sensitivity of early ring YL, Dao M, Lim CT, Renia L, Tan KS, Russell B. A Basis for Rapid stage Plasmodium falciparum to artemisinins. J Cell Sci. 2016 Jan Clearance of Circulating Ring-Stage Malaria Parasites by the 15;129(2):406-16. doi: 10.1242/jcs.178830. Epub 2015 Dec 16. Spiroindolone KAE609. J Infect Dis. 2016 Jan 1;213(1):100-4. doi: 10.1093/infdis/jiv358. Epub 2015 Jul 1. Xu G, Nolder D, Reboud J, Oguike MC, van Schalkwyk DA, Sutherland CJ, Cooper JM. Paper-Origami-Based Multiplexed Zhang X, Kadir KA, Quintanilla-Zariñan LF, Villano J, Houghton P, Malaria Diagnostics from Whole Blood. Angew Chem Int Ed Engl. Du H, Singh B, Smith DG. Distribution and prevalence of malaria 2016 Dec 5;55(49):15250-15253. doi: 10.1002/anie.201606060. parasites among long-tailed macaques (Macaca fascicularis) in Epub 2016 Aug 24. regional populations across Southeast Asia. Malar J. 2016 Sep 2;15(1):450. doi: 10.1186/s12936-016-1494-0. Yang T, Xie SC, Cao P, Giannangelo C, McCaw J, Creek DJ, Charman SA, Klonis N, Tilley L. Comparison of the Exposure Time Dependence of the Activities of Synthetic Ozonide Antimalarials and Dihydroartemisinin against K13 Wild-Type and Mutant Food and Water Plasmodium falciparum Strains. Antimicrob Agents Chemother. 2016 Jul 22;60(8):4501-10. doi: 10.1128/AAC.00574-16. Print Al-Riyami S, Ioannidou E, Koehler AV, Hussain MH, Al-Rawahi 2016 Aug. AH, Giadinis ND, Lafi SQ, Papadopoulos E,Jabbar A. Genetic characterisation of Taenia multiceps cysts from ruminants in Yin M, Li H, Blair D, Xu B, Feng Z, Hu W. Temporal genetic diversity Greece. Infect Genet Evol. 2016 Mar;38:110-6. doi: 10.1016/j. of Schistosoma japonicum in two endemic sites in China revealed meegid.2015.12.008. Epub 2015 Dec 1 by microsatellite markers. Parasit Vectors. 2016 Jan 22;9:36. doi: 10.1186/s13071-016-1326-7. Alvarez Rojas CA, Scheerlinck JP, Ansell BR, Hall RS, Gasser RB, Jex AR. Time-Course Study of the Transcriptome of Peripheral You H, Gobert GN, Du X, Pali G, Cai P, Jones MK, McManus Blood Mononuclear Cells (PBMCs) from Sheep Infected with DP. Functional characterisation of Schistosoma japonicum Fasciola hepatica. PLoS One. 2016 Jul 20;11(7):e0159194. doi: acetylcholinesterase. Parasit Vectors. 2016 Jun 10;9(1):328. doi: 10.1371/journal.pone.0159194. eCollection 2016 10.1186/s13071-016-1615-1. Anim-Baidoo I, Narh CA, Oddei D, Brown CA, Enweronu-Laryea C, Zeng J, Chen QW, Yu ZY, Zhang JR, Chen DL, Song C, Luo J, Bandoh B, Sampane-Donkor E, Armah G, Adjei AA, Adjei DN, Ayeh- Zhang C, Wang SL, Chen JP. Regulation of intrinsic apoptosis in Kumi PF, Gyan BA. Giardia lamblia infections in children in Ghana. cycloheximide-treated macrophages by the Sichuan human strain Pan Afr Med J. 2016 Jul 12;24:217. eCollection 2016. of Chinese Leishmania isolates. Acta Trop. 2016 Jan;153:101-10. doi: 10.1016/j.actatropica.2015.10.010. Epub 2015 Oct 23. Ansell BR, McConville MJ, Baker L, Korhonen PK, Emery SJ, Svärd SG, Gasser RB, Jex AR. Divergent Transcriptional Responses Zhang R, Lee WC, Lau YL, Albrecht L, Lopes SC, Costa FT, to Physiological and Xenobiotic Stress in Giardia duodenalis. Suwanarusk R, Nosten F, Cooke BM, Rénia L, Russell B. Antimicrob Agents Chemother. 2016 Sep 23;60(10):6034-45. doi:

Page 75 Appendix 1

10.1128/AAC.00977-16. Print 2016 Oct. communities in Timor-Leste. Int J Parasitol. 2016 Nov;46(12):771- 779. doi: 10.1016/j.ijpara.2016.07.005. Epub 2016 Sep 8. Anstead CA, Batterham P, Korhonen PK, Young ND, Hall RS, Bowles VM, Richards S, Scott MJ, Gasser RB. A blow to the Casteriano A, Molini U, Kandjumbwa K, Khaiseb S, Frey CF, Šlapeta fly - Lucilia cuprina draft genome and transcriptome to support J Novel genotype of Tritrichomonas foetus from cattle in Southern advances in biology and biotechnology. Biotechnol Adv. 2016 Sep- Africa. Parasitology. 2016 Dec;143(14):1954-1959. Epub 2016 Sep Oct;34(5):605-20. doi: 10.1016/j.biotechadv.2016.02.009. Epub 9. 2016 Mar 2. Review. Chand KK, Lee KM, Lavidis NA, Rodriguez-Valle M, Ijaz Ashraf S, Prichard R. Ivermectin exhibits potent anti-mitotic H, Koehbach J, Clark RJ, Lew-Tabor A, Noakes PG. Tick activity. Vet Parasitol. 2016 Aug 15;226:1-4. doi: 10.1016/j. holocyclotoxins trigger host paralysis by presynaptic inhibition. Sci vetpar.2016.06.015. Epub 2016 Jun 11. Rep. 2016 Jul 8;6:29446. doi: 10.1038/srep29446.

Avais M, Rashid G, Ijaz M, Khan MA, Nasir A, Jahanzaib MS, Cortés A, Sotillo J, Muñoz-Antolí C, Martín-Grau C, Esteban JG, Khan JA, Hameed S, Reichel MP. Evaluation of furazolidone, Toledo R. Resistance against Echinostoma caproni (Trematoda) sulfadimidine and amprolium to treat coccidiosis in Beetal goats secondary infections in mice is not dependent on the ileal protein under field conditions. Pak J Pharm Sci. 2016 Mar;29(2):485-7. production. J Proteomics. 2016 May 17;140:37-47. doi: 10.1016/j. jprot.2016.03.034. Epub 2016 Mar 31 Bernigaud C, Fang F, Fischer K, Lespine A, Aho LS, Dreau D, Kelly A, Sutra JF, Moreau F, Lilin T, Botterel F, Guillot J, Chosidow O. Cortés A, Sotillo J, Muñoz-Antolí C, Trelis M, Esteban JG, Toledo Preclinical Study of Single-Dose Moxidectin, a New Oral Treatment R. Definitive host influences the proteomic profile of excretory/ for Scabies: Efficacy, Safety, and Pharmacokinetics Compared to secretory products of the trematode Echinostoma caproni. Parasit Two-Dose Ivermectin in a Porcine Model. PLoS Negl Trop Dis. 2016 Vectors. 2016 Mar 31;9:185. doi: 10.1186/s13071-016-1465-x. Oct 12;10(10):e0005030. doi: 10.1371/journal.pntd.0005030. eCollection 2016 Oct. Cross M, Klepzig E, Dallaston M, Young ND, Bailey UM, Mason L, Jones MK, Gasser RB, Hofmann A. Exploring the molecular Brennan A, Donahoe SL, Beatty JA, Belov K, Lindsay S, Briscoe mechanisms of parasite-host interactions with a view towards new KA, Šlapeta J, Barrs VR. Comparison of genotypes of Toxoplasma therapeutics and vaccines. Postepy Biochem. 2016;62(3):370-376. gondii in domestic cats from Australia with latent infection or Review. clinical toxoplasmosis. Vet Parasitol. 2016 Sep 15;228:13-16. doi: 10.1016/j.vetpar.2016.06.008. Epub 2016 Jun 6. David MA, Orlowski S, Prichard RK, Hashem S, André F, Lespine A. In silico analysis of the binding of anthelmintics to Caenorhabditis Brown S, Pedley KC, Simcock DC. Estimation of Surface Area elegansP-glycoprotein 1.Int J Parasitol Drugs Drug Resist. 2016 and Volume of a Nematode from Morphometric Data. Scientifica Dec;6(3):299-313. doi: 10.1016/j.ijpddr.2016.09.001. Epub 2016 (Cairo). 2016;2016:6767538. doi: 10.1155/2016/6767538. Epub Sep 15. 2016 Mar 27. Emery DL, Hunt PW, Le Jambre LF. Haemonchus contortus: Campbell SJ, Nery SV, D’Este CA, Gray DJ, McCarthy JS, Traub the then and now, and where to from here? Int J Parasitol. 2016 RJ, Andrews RM, Llewellyn S, Vallely AJ, Williams GM, Amaral S, Nov;46(12):755-769. doi: 10.1016/j.ijpara.2016.07.001. Epub Clements AC. Water, sanitation and hygiene related risk factors for 2016 Sep 13. Review. soil-transmitted helminth and Giardia duodenalis infections in rural

Page 76 Appendix 1

Emery SJ, Lacey E, Haynes PA. Quantitative proteomics in Giardia affected by infection salmon anaemia virus (ISAV), Piscirickettsia duodenalis-Achievements and challenges. Mol Biochem Parasitol. salmonis, and Neoparamoeba perurans. Ocean & Coastal 2016 Aug;208(2):96-112. doi: 10.1016/j.molbiopara.2016.07.002. Management 123: 74-83. Epub 2016 Jul 19. Godoy P, Che H, Beech RN, Prichard RK. Characterisation of Emery SJ, Mirzaei M, Vuong D, Pascovici D, Chick JM, Lacey E, P-glycoprotein-9.1 in Haemonchus contortus. Parasit Vectors. Haynes PA. Induction of virulence factors in Giardia duodenalis 2016 Jan 28;9:52. doi: 10.1186/s13071-016-1317-8. independent of host attachment. Sci Rep. 2016 Feb 12;6:20765. doi: 10.1038/srep20765. Guan G, Korhonen PK, Young ND, Koehler AV, Wang T, Li Y, Liu Z, Luo J, Yin H, Gasser RB. Genomic resources for a unique, low- Frölich S, Wallach M. Use of fluorescent nanoparticles to virulence Babesia taxon from China. Parasit Vectors. 2016 Oct investigate nutrient acquisition by developing Eimeria maxima 27;9(1):564. macrogametocytes. Sci Rep. 2016 Jun 29;6:29030. doi: 10.1038/ srep29030. Hammer JF, Jenkins C, Bogema D, Emery D. Mechanical transfer of Theileria orientalis: possible roles of biting arthropods, colostrum Garcia HH, Gonzalez AE, Tsang VC, O’Neal SE, Llanos-Zavalaga F, and husbandry practices in disease transmission. Parasit Vectors. Gonzalvez G, Romero J, Rodriguez S, Moyano LM, Ayvar V, Diaz A, 2016 Jan 22;9:34. doi: 10.1186/s13071-016-1323-x. Hightower A, Craig PS, Lightowlers MW, Gauci CG, Leontsini E, Gilman RH; Cysticercosis Working Group in Peru. Elimination of Hanadate Y, Saito-Nakano Y, Nakada-Tsukui K, Nozaki T. Taenia solium Transmission in Northern Peru. N Engl J Med. 2016 Endoplasmic reticulum-resident Rab8A GTPase is involved in Jun 16;374(24):2335-44. doi: 10.1056/NEJMoa1515520. phagocytosis in the protozoan parasite Entamoeba histolytica. Cell Microbiol. 2016 Oct;18(10):1358-73. doi: 10.1111/cmi.12570. Garcia-Campos A, Ravidà A, Nguyen DL, Cwiklinski K, Dalton JP, Epub 2016 Apr 4. Hokke CH, O’Neill S, Mulcahy G. Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Hansen EP, Kringel H, Thamsborg SM, Jex A, Nejsum P. Profiling Mannosidic and Paucimannosidic N-glycans. PLoS Negl Trop Dis. circulating miRNAs in serum from pigs infected with the porcine 2016 May 3;10(5):e0004688. doi: 10.1371/journal.pntd.0004688. whipworm, Trichuris suis. Vet Parasitol. 2016 Jun 15;223:30-3. doi: eCollection 2016 May. 10.1016/j.vetpar.2016.03.025. Epub 2016 Apr 6.

Gebrekidan H, Gasser RB, Baneth G, Yasur-Landau D, Nachum- Hess J, Patra M, Jabbar A, Pierroz V, Konatschnig S, Spingler B, Biala Y, Hailu A, Jabbar A. Molecular characterization of Theileria Ferrari S, Gasser RB, Gasser G Assessment of the nematocidal orientalis from cattle in Ethiopia. Ticks Tick Borne Dis. 2016 activity of metallocenyl analogues of monepantel. Dalton Trans. Jul;7(5):742-7. doi: 10.1016/j.ttbdis.2016.03.005. Epub 2016 Mar 2016 Nov 28;45(44):17662-17671. Epub 2016 Oct 17. 10. Hess J, Patra M, Rangasamy L, Konatschnig S, Blacque O, Jabbar González, L. Spatial and temporal distribution of A, Mac P, Jorgensen EM, Gasser RB, Gasser G. Organometallic Neoparamoeba perurans in a tank recirculation system during Derivatization of the Nematocidal Drug Monepantel Leads to experimental AGD challenge. Aquaculture 450:363-368. Promising Antiparasitic Drug Candidates. Chemistry. 2016 Nov 7;22(46):16602-16612. doi: 10.1002/chem.201602851. Epub Gonzalez, L., C Robles, M. Cortez San Martín. Management 2016 Oct 5. issues regarding caligidosis treatments on salmon farms in Chile

Page 77 Appendix 1

Hijjawi N, Mukbel R, Yang R, Ryan U. Genetic characterization Korhonen PK, Pozio E, La Rosa G, Chang BC, Koehler AV, Hoberg of Cryptosporidium in animal and human isolates from Jordan. EP, Boag PR, Tan P, Jex AR, Hofmann A, Sternberg PW, Young ND, Vet Parasitol. 2016 Sep 15;228:116-120. doi: 10.1016/j. Gasser RB. Phylogenomic and biogeographic reconstruction of vetpar.2016.08.015. Epub 2016 Aug 21. the Trichinella complex. Nat Commun. 2016 Feb 1;7:10513. doi: 10.1038/ncomms10513. Hijjawi N, Yang R, Mukbel R, Yassin Y, Mharib T, Ryan U. First genetic characterisation of Giardia in human isolates from Jordan. Korhonen PK, Young ND, Gasser RB. Making sense of genomes of parasitic worms: Tackling bioinformatic challenges. Parasitol Res. 2016 Oct;115(10):3723-9. doi: 10.1007/s00436- Biotechnol Adv. 2016 Sep-Oct;34(5):663-86. doi: 10.1016/j. 016-5132-0. Epub 2016 May 20. biotechadv.2016.03.001. Epub 2016 Mar 5. Review.

Jacobson C, Williams A, Yang R, Ryan U, Carmichael I, Kumarasingha R, Karpe AV, Preston S, Yeo TC, Lim DS, Tu CL, Campbell AJ, Gardner GE. Greater intensity and frequency of Luu J, Simpson KJ, Shaw JM, Gasser RB, Beale DJ, Morrison PD, Cryptosporidium and Giardia oocyst shedding beyond the Palombo EA, Boag PR. Metabolic profiling and in vitro assessment neonatal period is associated with reductions in growth, carcase of anthelmintic fractions of Picria fel-terrae Lour. Int J Parasitol weight and dressing efficiency in sheep. Vet Parasitol. 2016 Sep Drugs Drug Resist. 2016 Dec;6(3):171-178. doi: 10.1016/j. 15;228:42-51. doi: 10.1016/j.vetpar.2016.08.003. Epub 2016 Aug ijpddr.2016.08.002. Epub 2016 Aug 26. 3. Kumarasingha R, Preston S, Yeo TC, Lim DS, Tu CL, Palombo EA, Jenkins C, Bogema DR. Factors associated with seroconversion to Shaw JM, Gasser RB, Boag PR. Anthelmintic activity of selected the major piroplasm surface protein of the bovine haemoparasite ethno-medicinal plant extracts on parasitic stages of Haemonchus Theileria orientalis. Parasit Vectors. 2016 Feb 25;9:106. doi: contortus. Parasit Vectors. 2016 Apr 1;9:187. doi: 10.1186/s13071- 10.1186/s13071-016-1395-7. 016-1458-9.

Kelley JM, Elliott TP, Beddoe T, Anderson G, Skuce P, Spithill Li FC, Gasser RB, Lok JB, Korhonen PK, He L, Di WD, Yin FY, TW. Current Threat of Triclabendazole Resistance in Fasciola Zhou R, Zhou YQ, Zhao JL, Hu M. Molecular characterization of hepatica. Trends Parasitol. 2016 Jun;32(6):458-69. doi: 10.1016/j. the Haemonchus contortus phosphoinositide-dependent protein pt.2016.03.002. Epub 2016 Apr 2. Review. kinase-1 gene (Hc-pdk-1). Parasit Vectors. 2016 Feb 3;9:65. doi: 10.1186/s13071-016-1351-6. Kenngott EE, Cole S, Hein WR, Hoffmann U, Lauer U, Maass D, Moore L, Pfeil J, Rosanowski S, Shoemaker CB, Umair S, Volkmer Lightowlers MW, Donadeu M, Elaiyaraja M, Maithal K, Kumar R, Hamann A, Pernthaner A. Identification of Targeting Peptides KA, Gauci CG, Firestone SM, Sarasola P, Rowan TG. Anamnestic for Mucosal Delivery in Sheep and Mice. Mol Pharm. 2016 Jan responses in pigs to the Taenia solium TSOL18 vaccine 4;13(1):202-10. doi: 10.1021/acs.molpharmaceut.5b00635. Epub and implications for control strategies. Parasitology. 2016 2015 Dec 1. Apr;143(4):416-20. doi: 10.1017/S0031182016000202. Epub 2016 Feb 19. Koehler AV, Haydon SR, Jex AR, Gasser RB. Cryptosporidium and Giardia taxa in faecal samples from animals in catchments Liu GH, Nadler SA, Liu SS, Podolska M, D’Amelio S, Shao R, supplying the city of Melbourne with drinking water (2011 to Gasser RB, Zhu XQ. Mitochondrial Phylogenomics yields Strongly 2015). Parasit Vectors. 2016 Jun 1;9(1):315. doi: 10.1186/s13071- Supported Hypotheses for Ascaridomorph Nematodes Sci Rep. 016-1607-1 2016 Dec 16;6:39248. doi: 10.1038/srep39248.

Page 78 Appendix 1

Mani T, Bourguinat C, Keller K, Ashraf S, Blagburn B, Prichard CS, Menon S, Mills B, Pullins A, Zinser E, Woods DJ, Meeus P. RK. Interaction of macrocyclic lactones with a Dirofilaria immitis Discovery of sarolaner: A novel, orally administered, broad- P-glycoprotein. Int J Parasitol. 2016 Sep;46(10):631-40. doi: spectrum, isoxazoline ectoparasiticide for dogs. Vet Parasitol. 2016 10.1016/j.ijpara.2016.04.004. Epub 2016 May 9. May 30;222:3-11. doi: 10.1016/j.vetpar.2016.02.019. Epub 2016 Feb 23. Mani T, Bourguinat C, Keller K, Carreton E, Peregrine A, Prichard RK. Polymorphism in ion channel genes of Dirofilaria immitis: Mofiz E,Holt DC, Seemann T, Currie BJ, Fischer K, Papenfuss AT. Relevant knowledge for future anthelmintic drug design. Int Genomic resources and draft assemblies of the human and porcine J Parasitol Drugs Drug Resist. 2016 Dec;6(3):343-355. doi: varieties of scabies mites, Sarcoptes scabiei var. hominis and var. 10.1016/j.ijpddr.2016.06.003. Epub 2016 Jul 1. suis. Gigascience. 2016 Jun 2;5(1):23. doi: 10.1186/s13742-016- 0129-2. Martin MB, Bruce NL, Nowak BF. Review of the fish-parasitic genus Cymothoa Fabricius, 1793 (Crustacea: Isopoda: Mohandas N, Hu M, Stroehlein AJ, Young ND, Sternberg PW, Cymothoidae) from Australia. Zootaxa. 2016 Jun 3;4119(1):1-72. Lok JB, Gasser RB. Reconstruction of the insulin-like signalling doi: 10.11646/zootaxa.4119.1.1. pathway of Haemonchus contortus. Parasit Vectors. 2016 Feb 3;9:64. doi: 10.1186/s13071-016-1341-8. Martínez-Hoyos M, García-Bustos JF et al Antitubercular drugs for an old target: GSK693 as a promising InhA direct Mohandas N, Young ND, Jabbar A, Korhonen PK, Koehler AV, inhibitor. EBioMedicine. 2016 Jun;8:291-301. doi: 10.1016/j. Amani P, Hall RS, Sternberg PW, Jex AR, Hofmann A, Gasser ebiom.2016.05.006. Epub 2016 May 8. RB. The barber’s pole worm CAP protein superfamily--A basis for fundamental discovery and biotechnology advances. McAllister MM. Diagnosis and Control of Bovine Neosporosis. Biotechnol Adv. 2015 Dec;33(8):1744-54. doi: 10.1016/j. Vet Clin North Am Food Anim Pract. 2016 Jul;32(2):443-63. doi: biotechadv.2015.07.003. Epub 2015 Jul 31. Review. 10.1016/j.cvfa.2016.01.012. Epub 2016 May 6. Review. Mohandas N, Young ND, Jabbar A, Korhonen PK, Koehler AV, McCusker P, McVeigh P, Rathinasamy V, Toet H, McCammick Hall RS, Hu M, Hofmann A, Gasser RB. The complement of family E, O’Connor A, Marks NJ, Mousley A, Brennan GP, Halton DW, M1 aminopeptidases of Haemonchus contortus--Biotechnological Spithill TW, Maule AG. Stimulating Neoblast-Like Cell Proliferation implications. Biotechnol Adv. 2016 Mar-Apr;34(2):65-76. doi: in Juvenile Fasciola hepatica Supports Growth and Progression 10.1016/j.biotechadv.2015.10.003. Epub 2015 Oct 24. Review. towards the Adult Phenotype In Vitro. PLoS Negl Trop Dis. 2016 Sep 13;10(9):e0004994. doi: 10.1371/journal.pntd.0004994. Muñoz-Antoli C, Cortés A, Santano R, Sotillo J, Esteban JG, Toledo eCollection 2016 Sep. R. Interleukin-25 Induces Resistance Against Intestinal Trematodes. Sci Rep. 2016 Sep 23;6:34142. doi: 10.1038/srep34142. McNulty SN, Strübe C, Rosa BA, Martin JC, Tyagi R, Choi YJ, Wang Q, Hallsworth Pepin K, Zhang X, Ozersky P, Wilson RK, Sternberg Mutebi F, Krücken J, Feldmeier H, Waiswa C, Mencke N, von PW, Gasser RB, Mitreva M. Dictyocaulus viviparus genome, Samson-Himmelstjerna G. Tungiasis-associated morbidity in pigs variome and transcriptome elucidate lungworm biology and and dogs in endemic villages of Uganda. Parasit Vectors. 2016 Jan support future intervention. Sci Rep. 2016 Feb 9;6:20316. doi: 27;9:44. doi: 10.1186/s13071-016-1320-0. 10.1038/srep20316. Mutebi F, Krücken J, Mencke N, Feldmeier H, von Samson- McTier TL, Chubb N, Curtis MP, Hedges L, Inskeep GA, Knauer Himmelstjerna G, Waiswa C. Two Severe Cases of Tungiasis in Goat

Page 79 Appendix 1

Kids in Uganda. J Insect Sci. 2016 Mar 24;16. pii: 34. doi: 10.1093/ infected with multiple Neospora caninum strains based on MS10 jisesa/iew016. Print 2016. microsatellite analysis. Vet Parasitol. 2016 May 15;221:134-8. doi: 10.1016/j.vetpar.2016.03.023. Epub 2016 Mar 29. Nor B, Young ND, Korhonen PK, Hall RS, Tan P, Lonie A, Gasser RB. Pipeline for the identification and classification of ion channels Preston S, Jabbar A, Gasser RB. A perspective on genomic- in parasitic flatworms. Parasit Vectors. 2016 Mar 16;9:155. doi: guided anthelmintic discovery and repurposing using 10.1186/s13071-016-1428-2. Haemonchus contortus. Infect Genet Evol. 2016 Jun;40:368-73. doi: 10.1016/j.meegid.2015.06.029. Epub 2015 Jul 3. Okello AL, Thomas L, Inthavong P, Ash A, Khamlome B, Keokamphet C, Newberry K, Gauci CG, Gabriël S, Dorny P, Preston S, Jabbar A, Nowell C, Joachim A, Ruttkowski B, Cardno Thompson RA, Lightowlers MW, Allen J. Assessing the impact T, Hofmann A, Gasser RB. Practical and low cost whole-organism of a joint human-porcine intervention package for Taenia solium motility assay: A step-by-step protocol. Mol Cell Probes. 2016 control: Results of a pilot study from northern Lao PDR. Acta Trop. Feb;30(1):13-7. doi: 10.1016/j.mcp.2015.08.005. Epub 2015 Sep 2016 Jul;159:185-91. doi: 10.1016/j.actatropica.2016.03.012. 10. Epub 2016 Mar 15. Preston S, Jiao Y, Jabbar A, McGee SL, Laleu B, Willis P, Wells TN, Ondrovics M, Gasser RB, Joachim A. Recent Advances Gasser RB. Screening of the ‘Pathogen Box’ identifies an approved in Elucidating Nematode Moulting - Prospects of Using pesticide with major anthelmintic activity against the barber’s pole Oesophagostomum dentatum as a Model. Adv Parasitol. worm. Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):329-334. 2016;91:233-64. doi: 10.1016/bs.apar.2015.09.001. Epub 2015 doi: 10.1016/j.ijpddr.2016.07.004. Epub 2016 Jul 28. Review. Nov 26. Review. Preston S, Luo J, Zhang Y, Jabbar A, Crawford S, Baell J, Hofmann Ong, C.T., Rodriguez Valle, M., Barrero, R.A., Hunter, A., Bellgard, A, Hu M, Zhou HB, Gasser RB. Selenophene and thiophene-core M.I., Lew-Tabor, A.E. Exploring the transcriptomic data of the estrogen receptor ligands that inhibit motility and development of Australian paralysis tick, Ixodes holocyclus. GSTF Journal of parasitic stages of Haemonchus contortus. Parasit Vectors. 2016 Veterinary Science (JVet) 3(1). Jun 16;9(1):346. doi: 10.1186/s13071-016-1612-4.

Paul AS, Moreira CK, Elsworth B, Allred DR, Duraisingh MT Ravidà A, Cwiklinski K, Aldridge AM, Clarke P, Thompson R, Extensive Shared Chemosensitivity between Malaria and Gerlach JQ, Kilcoyne M, Hokke CH, Dalton JP, O’Neill SM. Fasciola Babesiosis Blood-Stage Parasites. Antimicrob Agents Chemother. hepatica Surface Tegument: Glycoproteins at the Interface of 2016 Jul 22;60(8):5059-63. doi: 10.1128/AAC.00928-16. Print Parasite and Host. Mol Cell Proteomics. 2016 Oct;15(10):3139- 2016 Aug. 3153. Epub 2016 Jul 27.

Pennacchi Y, Shirakashi S, Nowak BF, Bridle AR Immune reactivity Raza A, Bagnall NH, Jabbar A, Kopp SR, Kotze AC. Increased in early life stages of sea-cage cultured Pacific bluefin tuna naturally expression of ATP binding cassette transporter genes following infected with blood flukes from genus Cardicola (Trematoda: exposure of Haemonchus contortus larvae to a high concentration Aporocotylidae). Fish Shellfish Immunol. 2016 Nov;58:490-499. of monepantel in vitro. Parasit Vectors. 2016 Sep 29;9(1):522. doi: 10.1016/j.fsi.2016.09.060. Epub 2016 Oct 1. Raza A, Kopp SR, Bagnall NH, Jabbar A, Kotze AC. Effects of Prandini da Costa Reis R, Crisman R, Roser M, Malik R, Šlapeta in vitro exposure to ivermectin and levamisole on the expression J. Neonatal neosporosis in a 2-week-old Bernese mountain dog patterns of ABC transporters in Haemonchus contortus larvae. Int J

Page 80 Appendix 1

Parasitol Drugs Drug Resist. 2016 Aug;6(2):103-15. doi: 10.1016/j. Shamsi S, Ghadam M, Suthar J, Ebrahimzadeh Mousavi H, ijpddr.2016.03.001. Epub 2016 Mar 23. Soltani M, Mirzargar S. Occurrence of ascaridoid nematodes in selected edible fish from the Persian Gulf and description of Raza A, Kopp SR, Kotze AC. Synergism between ivermectin and Hysterothylacium larval type XV and Hysterothylacium persicum the tyrosine kinase/P-glycoprotein inhibitor crizotinib against n. sp. (Nematoda: Raphidascarididae). Int J Food Microbiol. 2016 Haemonchus contortus larvae in vitro. Vet Parasitol. 2016 Aug Nov 7;236:65-73. doi: 10.1016/j.ijfoodmicro.2016.07.006. Epub 30;227:64-8. doi: 10.1016/j.vetpar.2016.07.026. Epub 2016 Jul 22. 2016 Jul 11.

Raza A, Lamb J, Chambers M, Hunt PW, Kotze AC. Larval Shamsi S, Suthar J. Occurrence of Terranova larval types development assays reveal the presence of sub-populations (Nematoda: Anisakidae) in Australian marine fish with comments showing high- and low-level resistance in a monepantel (Zolvix®)- on their specific identities. PeerJ. 2016 Mar 15;4:e1722. doi: resistant isolate of Haemonchus contortus. Vet Parasitol. 2016 Apr 10.7717/peerj.1722. eCollection 2016. 15;220:77-82. doi: 10.1016/j.vetpar.2016.02.031. Epub 2016 Mar 3. Shamsi S, Suthar J.. A revised method of examining fish for infection with zoonotic nematode larvae. Int J Food Microbiol. 2016 Read E, Edwards J, Deseo M, Rawlin G, Rochfort S. Current Jun 16;227:13-6. doi: 10.1016/j.ijfoodmicro.2016.03.023. Epub Understanding of Acute Bovine Liver Disease in Australia. Toxins 2016 Mar 24. (Basel). 2016 Dec 26;9(1). pii: E8. doi: 10.3390/toxins9010008. Review. Srivastava VK, Chandra M, Saito-Nakano Y, Nozaki T, Datta S. Crystal Structure Analysis of Wild Type and Fast Hydrolyzing Reichel MP, Lanyon SR, Hill FI. Moving past serology: Diagnostic Mutant of EhRabX3, a Tandem Ras Superfamily GTPase from options without serum. Vet J. 2016 Sep;215:76-81. doi: 10.1016/j. Entamoeba histolytica. J Mol Biol. 2016 Jan 16;428(1):41-51. doi: tvjl.2016.04.010. Epub 2016 Apr 22. Review. 10.1016/j.jmb.2015.11.003. Epub 2015 Nov 10.

Ryan U, Paparini A, Monis P, Hijjawi N. It’s official - Stark JS, Corbett PA, Dunshea G, Johnstone G, King C, Mondon Cryptosporidium is a gregarine: What are the implications for JA, Power ML, Samuel A, Snape I, Riddle M. The environmental the water industry? Water Res. 2016 Nov 15;105:305-313. doi: impact of sewage and wastewater outfalls in Antarctica: An 10.1016/j.watres.2016.09.013. Epub 2016 Sep 9. Review. example from Davis station, East Antarctica. Water Res. 2016 Nov 15;105:602-614. doi: 10.1016/j.watres.2016.09.026. Epub 2016 Samsing F, Johnsen I, Stien LH, Oppedal F, Albretsen J, Asplin Sep 18. L, Dempster T. Predicting the effectiveness of depth-based technologies to prevent salmon lice infection using a dispersal Stroehlein AJ, Young ND, Korhonen PK, Chang BC, Sternberg model. Prev Vet Med. 2016 Jul 1;129:48-57. doi: 10.1016/j. PW, La Rosa G, Pozio E, Gasser RB. Analyses of Compact prevetmed.2016.05.010. Epub 2016 May 20. Trichinella Kinomes Reveal a MOS-Like Protein Kinase with a Unique N-Terminal Domain. G3 (Bethesda). 2016 Sep 8;6(9):2847- Schetters T, Bishop R, Crampton M, Kopáček P, Lew-Tabor A, 56. doi: 10.1534/g3.116.032961. Maritz-Olivier C, Miller R, Mosqueda J, Patarroyo J, Rodriguez- Valle M, Scoles GA, de la Fuente J. Cattle tick vaccine researchers Swastika K, Dharmawan NS, Suardita IK, Kepeng IN, Wandra T, join forces in CATVAC. Parasit Vectors. 2016 Feb 24;9:105. doi: Sako Y, Okamoto M, Yanagida T, Sasaki M, Giraudoux P, Nakao M, 10.1186/s13071-016-1386-8. Yoshida T, Eka Diarthini LP, Sudarmaja IM, Purba IE, Budke CM, Ito A. Swine cysticercosis in the Karangasem district of Bali, Indonesia:

Page 81 Appendix 1

An evaluation of serological screening methods. Acta Trop. 2016 Yap NJ, Koehler AV, Ebner J, Tan TK, Lim YA, Gasser RB. Molecular Nov;163:46-53. doi: 10.1016/j.actatropica.2016.07.022. Epub analysis of Cryptosporidium from cattle from five states of 2016 Jul 29. Peninsular Malaysia. Mol Cell Probes. 2016 Feb;30(1):39-43. doi: 10.1016/j.mcp.2016.01.002. Epub 2016 Jan 9. Thompson RC, Ash A. Molecular epidemiology of Giardia and Cryptosporidium infections. Infect Genet Evol. 2016 Jun;40:315- Yim WT, Bhandari B, Jackson L, James P. Repellent effects of 23. doi: 10.1016/j.meegid.2015.09.028. Epub 2015 Oct 11. Melaleuca alternifolia (tea tree) oil against cattle tick larvae (Rhipicephalus australis) when formulated as emulsions and Vannella KM, Ramalingam TR, Hart KM, de Queiroz Prado R, in β-cyclodextrin inclusion complexes. Vet Parasitol. 2016 Jul Sciurba J, Barron L, Borthwick LA, Smith AD, Mentink-Kane 30;225:99-103. doi: 10.1016/j.vetpar.2016.06.007. Epub 2016 Jun M, White S, Thompson RW, Cheever AW, Bock K, Moore I, Fitz 3. LJ, Urban JF Jr, Wynn TA. Acidic chitinase primes the protective immune response to gastrointestinal nematodes. Nat Immunol. Yin F, Gasser RB, Li F, Bao M, Huang W, Zou F, Zhao G, Wang C, 2016 May;17(5):538-44. doi: 10.1038/ni.3417. Epub 2016 Apr 4. Yang X, Zhou Y, Zhao J, Fang R, Hu M. Population structure of Haemonchus contortus from seven geographical regions in China, Walker RA, Niepceron A, Ramakrishnan C, Sedano L, Hehl AB, determined on the basis of microsatellite markers. Parasit Vectors. Brossier F, Smith NC. Discovery of a tyrosine-rich sporocyst wall 2016 Nov 15;9(1):586. protein in Eimeria tenella. Parasit Vectors. 2016 Mar 2;9:124. doi: 10.1186/s13071-016-1410-z. Zahedi A, Monis P, Aucote S, King B, Paparini A, Jian F, Yang R, Oskam C, Ball A, Robertson I, Ryan U. Zoonotic Cryptosporidium Weaver ER, Agius PA, Veale H, Dorning K, Hlang TT, Aung PP, Species in Animals Inhabiting Sydney Water Catchments. PLoS Fowkes FJ, Hellard ME. Water, Sanitation, and Hygiene Facilities One. 2016 Dec 14;11(12):e0168169. doi: 10.1371/journal. and Hygiene Practices Associated with Diarrhea and Vomiting pone.0168169. eCollection 2016. in Monastic Schools, Myanmar. Am J Trop Med Hyg. 2016 Aug 3;95(2):278-87. doi: 10.4269/ajtmh.15-0290. Epub 2016 Jun 20. Zahedi A, Phasey J, Boland T, Ryan U. First report of Cryptosporidium species in farmed and wild buffalo from the Woon SA, Yang R, Ryan U, Boan P, Prentice D. Chronic Northern Territory, Australia. Parasitol Res. 2016 Mar;115(3):1349- Cystoisospora belli infection in an immunocompetent Myanmar 53. doi: 10.1007/s00436-016-4901-0. Epub 2016 Jan 13. refugee - microscopy is not sensitive enough. BMC Infect Dis. 2016 May 21;16:221. doi: 10.1186/s12879-016-1558-3. Zhang, B., Sambono, J.L. Morgan, J.A.T, Venus, B., Rolls, P., Lew- Tabor, A.E. An evaluation of quantitative PCR assays (TaqMan Xu T, Lew-Tabor A, Rodriguez-Valle M. Effective inhibition and SYBR Green) for the detection of Babesia bigemina and of thrombin by Rhipicephalus microplus serpin-15 (RmS-15) Babesia bovis, and a novel fluorescent-ITS1-PCR capillary obtained in the yeast Pichia pastoris. Ticks Tick Borne Dis. 2016 electrophoresis method for genotyping B. bovis isolates. Feb;7(1):180-7. doi: 10.1016/j.ttbdis.2015.09.007. Epub 2015 Oct Veterinary Sciences 3 (3), 23. 22. Zhang Z, Gasser RB, Yang X, Yin F, Zhao G, Bao M, Pan B, Yang X, Gasser RB, Fang R, Zeng J, Zhu K, Qi M, Zhang Z, Tan L, Lei Huang W, Wang C, Zou F, Zhou Y, Zhao J, Fang R, Hu M. Two W, Zhou Y, Zhao J, Hu M. First survey of parasitic helminths of goats benzimidazole resistance-associated SNPs in the isotype-1 along the Han River in Hubei Province, China. Acta Parasitol. 2016 β-tubulin gene predominate in Haemonchus contortus Sep 1;61(3):602-6. doi: 10.1515/ap-2016-0080. populations from eight regions in China. Int J Parasitol Drugs Drug

Page 82 Appendix 1

Resist. 2016 Dec;6(3):199-206. doi: 10.1016/j.ijpddr.2016.10.001. clade causing trypanosomosis in an Australian little red flying fox Epub 2016 Oct 5. (Pteropus scapulatus). Vet Parasitol. 2016 Jun 15;223:214-21. doi: 10.1016/j.vetpar.2016.05.002. Epub 2016 May 3.

Barton DP, Morgan JA. A morphological and genetic description of A Changing Environment pentastomid infective nymphs belonging to the family Sebekidae Sambon, 1922 in fish in Australian waters. Folia Parasitol (Praha). Aitken EH, Bueno MG, Dos Santos Ortolan L, Alvaréz JM, Pissinatti 2016 Jul 29;63. pii: 2016.026. doi: 10.14411/fp.2016.026. A, Kierulff MC, Catão-Dias JL, Epiphanio S. Survey of Plasmodium in the golden-headed lion tamarin (Leontopithecus chrysomelas) Beveridge, I. The gastro-intestinal helminth parasites of the living in urban Atlantic forest in Rio de Janeiro, Brazil Malar J. 2016 swamp wallaby, Wallabia bicolor (Desmarest) (Marsupialia: Feb 17;15:93. doi: 10.1186/s12936-016-1155-3. Macropodidae), and their regional distribution. Transactions of the Royal Society of South Australia 140: 203-227. Andres MJ, Pulis EE, Overstreet RM. Description of three species of Isorchis (Digenea: Atractotrematidae) from Australia. Acta Blair D, Nawa Y, Mitreva M, Doanh PN. Gene diversity and genetic Parasitol. 2016 Sep 1;61(3):590-601. doi: 10.1515/ap-2016-0079. variation in lung flukes (genus Paragonimus). Trans R Soc Trop Med Hyg. 2016 Jan;110(1):6-12. doi: 10.1093/trstmh/trv101. Review. Andriantsoanirina V, Fang F, Ariey F, Izri A, Foulet F, Botterel F, Bernigaud C, Chosidow O, Huang W, Guillot J, Durand R. Are Blasco-Costa I, Cutmore SC, Miller TL, Nolan MJ. Molecular humans the initial source of canine mange? Parasit Vectors. 2016 approaches to trematode systematics: ‘best practice’ and Mar 25;9:177. doi: 10.1186/s13071-016-1456-y. implications for future study. Syst Parasitol. 2016 Mar;93(3):295- 306. doi: 10.1007/s11230-016-9631-2. Epub 2016 Feb 22. Review. Austen JM, Paparini A, Reid SA, Friend JA, Ditcham WG, Ryan U. Molecular characterization of native Australian trypanosomes Blasco-Costa I, Poulin R, Presswell B. Species of Apatemon in quokka (Setonix brachyurus) populations from Western Szidat, 1928 and Australapatemon Sudarikov, 1959 (Trematoda: Australia. Parasitol Int. 2016 Jun;65(3):205-8. doi: 10.1016/j. Strigeidae) from New Zealand: linking and characterising life parint.2015.12.005. Epub 2015 Dec 15. cycle stages with morphology and molecules. Parasitol Res. 2016 Jan;115(1):271-89. doi: 10.1007/s00436-015-4744-0. Epub 2015 Baird FJ, Su X, Aibinu I, Nolan MJ, Sugiyama H, Otranto D, Lopata Sep 18. AL, Cantacessi C. The Anisakis Transcriptome Provides a Resource for Fundamental and Applied Studies on Allergy-Causing Parasites. Born-Torrijos A, Poulin R, Pérez-Del-Olmo A, Culurgioni J, Raga PLoS Negl Trop Dis. 2016 Jul 29;10(7):e0004845. doi: 10.1371/ JA, Holzer AS. An optimised multi-host trematode life cycle: fishery journal.pntd.0004845. eCollection 2016 Jul. discards enhance trophic parasite transmission to scavenging birds. Int J Parasitol. 2016 Oct;46(11):745-53. doi: 10.1016/j. Barbosa A, Austen J, Gillett A, Warren K, Paparini A, Irwin P, Ryan ijpara.2016.06.005. Epub 2016 Aug 1. U. First report of Trypanosoma vegrandis in koalas (Phascolarctos cinereus). Parasitol Int. 2016 Aug;65(4):316-8. doi: 10.1016/j. Botero A, Clode PL, Peacock C, Thompson RC. Towards a Better parint.2016.03.004. Epub 2016 Mar 10. Understanding of the Life Cycle of Trypanosoma copemani. Protist. 2016 Feb;167(1):82-92. doi: 10.1016/j.protis.2015.11.002. Epub Barbosa AD, Mackie JT, Stenner R, Gillett A, Irwin P, Ryan U. 2015 Nov 19. Trypanosoma teixeirae: A new species belonging to the T. cruzi

Page 83 Appendix 1

Botero A, Cooper C, Thompson CK, Clode PL, Rose K, Thompson Chilton NB, Huby-Chilton F, Koehler AV, Gasser RB, Beveridge RC. Morphological and Phylogenetic Description of Trypanosoma I. Phylogenetic relationships of species of the oesophageal noyesi sp. nov.: An Australian Wildlife Trypanosome within the T. parasitic nematode genera Cyclostrongylus and Spirostrongylus cruzi Clade. Protist. 2016 Nov;167(5):425-439. doi: 10.1016/j. (Strongyloidea: Chabertiidae: Cloacininae) with their wallaby hosts protis.2016.07.002. Epub 2016 Aug 2. (Marsupialia: Macropodidae). Mol Cell Probes. 2016 Apr;30(2):93- 9. doi: 10.1016/j.mcp.2016.01.004. Epub 2016 Jan 21. Bourguinat C, Che H, Mani T, Keller K, Prichard RK. ABC-B transporter genes in Dirofilaria immitis. Int J Parasitol Drugs Drug Chilton NB, Huby-Chilton F, Koehler AV, Gasser RB, Beveridge Resist. 2016 Aug;6(2):116-24. doi: 10.1016/j.ijpddr.2016.04.001. I. Detection of cryptic species of Rugopharynx (Nematoda: Epub 2016 Apr 8. Strongylida) from the stomachs of Australian macropodid marsupials. Int J Parasitol Parasites Wildl. 2016 Apr 19;5(2):124-33. Bray RA, Cribb TH, Littlewood DT, Waeschenbach A. The molecular doi: 10.1016/j.ijppaw.2016.04.003. eCollection 2016 Aug. phylogeny of the digenean family Opecoelidae Ozaki, 1925 and the value of morphological characters, with the erection of a new Choudhury A, Aguirre-Macedo ML, Curran SS, de Núñez MO, subfamily. Folia Parasitol (Praha). 2016 Apr 20;63. pii: 2016.013. Overstreet RM, de León GP, Santos CP. Trematode diversity in doi: 10.14411/fp.2016.013. freshwater fishes of the Globe II: ‘New World’. Syst Parasitol. 2016 Mar;93(3):271-82. doi: 10.1007/s11230-016-9632-1. Epub 2016 Bray RA, Diaz PE, Cribb TH. Knowledge of marine fish trematodes Feb 22. Review. of Atlantic and Eastern Pacific Oceans. Syst Parasitol. 2016 Mar;93(3):223-35. doi: 10.1007/s11230-016-9629-9. Epub 2016 Cirtwill AR, Stouffer DB,Poulin R, Lagrue C. Are parasite richness Feb 22. Review. and abundance linked to prey species richness and individual feeding preferences in fish hosts? Parasitology. 2016 Jan;143(1):75- Chapman PA, Owen H, Flint M, Traub RJ, Cribb TH, Mills PC. 86. doi: 10.1017/S003118201500150X. Epub 2015 Nov 17. Molecular Characterization of Coccidia Associated with an Epizootic in Green Sea Turtles (Chelonia mydas) in South East Clark NJ, Wells K, Dimitrov D, Clegg SM. Co-infections and Queensland, Australia. PLoS One. 2016 Feb 22;11(2):e0149962. environmental conditions drive the distributions of blood parasites doi: 10.1371/journal.pone.0149962. eCollection 2016. in wild birds. J Anim Ecol. 2016 Nov;85(6):1461-1470. doi: 10.1111/1365-2656.12578. Epub 2016 Aug 26. Chapman PA, Traub RJ, Kyaw-Tanner MT, Owen H, Flint M, Cribb TH, Mills PC. Terminal Restriction Fragment Length Cooper MK, Phalen DN, Donahoe SL, Rose K, Šlapeta J. The utility Polymorphism for the Identification of Spirorchiid Ova in Tissues of diversity profiling using Illumina 18S rRNA gene amplicon deep from the Green Sea Turtle, Chelonia mydas. PLoS One. 2016 sequencing to detect and discriminate Toxoplasma gondii among Aug 31;11(8):e0162114. doi: 10.1371/journal.pone.0162114. the cyst-forming coccidia. Vet Parasitol. 2016 Jan 30;216:38-45. eCollection 2016. doi: 10.1016/j.vetpar.2015.12.011. Epub 2015 Dec 18.

Chilton NB, Huby-Chilton F, Gasser RB, Koehler AV, Beveridge I. Cribb TH, Bray RA, Diaz PE, Huston DC, Kudlai O, Martin Pharyngostrongylus thylogale n. sp. (Nematoda: Strongylida) from SB, Yong RQ, Cutmore SC. Trematodes of fishes of the Indo- the stomachs of macropodid marsupials defined by morphological west Pacific: told and untold richness. Syst Parasitol. 2016 and molecular criteria. Syst Parasitol. 2016 Oct;93(8):749-60. doi: Mar;93(3):237-47. doi: 10.1007/s11230-016-9625-0. Epub 2016 10.1007/s11230-016-9661-9. Epub 2016 Sep 14. Feb 22. Review.

Page 84 Appendix 1

Cribb TH. Editorial: The biodiversity of trematodes of fishes. Syst Osei-Atweneboana MY, Grant WN. Parasitol. 2016 Mar;93(3):219-21. doi: 10.1007/s11230-016- 9628-x. Epub 2016 Feb 22. Drali R, Abi-Rached L, Boutellis A, Djossou F, Barker SC, Raoult D. Host switching of human lice to new world monkeys in South Curran SS, Phillips AJ, Overstreet RM, Benz GW, Henningsen AD. America. Infect Genet Evol. 2016 Apr;39:225-31. doi: 10.1016/j. Austrobdella cairae n. sp., an Oioxenous Marine Leech (Clitellata: meegid.2016.02.008. Epub 2016 Feb 8. Piscicolidae) from the Banded Guitarfish, Zapteryx exasperata, in the Northeastern Pacific Ocean. J Parasitol. 2016 Apr;102(2):179- Duarte AM, Jenkins TP, Latrofa MS, Giannelli A, Papadopoulos 86. doi: 10.1645/15-829. Epub 2016 Jan 22. E, de Carvalho LM, Nolan MJ, Otranto D, Cantacessi C. Helminth infections and gut microbiota - a feline perspective. Parasit Vectors. Cutmore SC, Diggles BK, Cribb TH. Transversotrema Witenberg, 2016 Dec 3;9(1):625. 1944 (Trematoda: Transversotrematidae) from inshore fishes of Australia: description of a new species and significant range Dybing NA, Jacobson C, Irwin P, Algar D, Adams PJ. Bartonella extensions for three congeners. Syst Parasitol. 2016 Sep;93(7):639- Species Identified in Rodent and Feline Hosts from Island and 52. doi: 10.1007/s11230-016-9658-4. Epub 2016 Aug 13. Mainland Western Australia. Vector Borne Zoonotic Dis. 2016 Apr;16(4):238-44. doi: 10.1089/vbz.2015.1902. Epub 2016 Feb da Silva FL, Dixon MW, Stack CM, Teuscher F, Taran E, Jones 22. MK, Lovas E, Tilley L, Brown CL, Trenholme KR, Dalton JP, Gardiner DL, Skinner-Adams TS. A Plasmodium falciparum S33 Fan YL, Lou ZZ, Li L, Yan HB, Liu QY, Zhan F, Li JQ, Liu CN, Cai JZ, proline aminopeptidase is associated with changes in erythrocyte Lei MT, Shi WG, Yang YR, McManus DP, Jia WZ. Genetic diversity deformability. Exp Parasitol. 2016 Oct;169:13-21. doi: 10.1016/j. in Echinococcus shiquicus from the plateau pika (Ochotona exppara.2016.06.013. Epub 2016 Jun 30 curzoniae) in Darlag County, Qinghai, China. Infect Genet Evol. 2016 Nov;45:408-414. doi: 10.1016/j.meegid.2016.06.016. Epub Delport TC, Power ML, Harcourt RG, Webster KN, Tetu SG. Colony 2016 Jun 6. Location and Captivity Influence the Gut Microbial Community Composition of the Australian Sea Lion (Neophoca cinerea). Appl Fayton TJ, Curran SS, Andres MJ, Overstreet RM, McAllister CT. Environ Microbiol. 2016 May 31;82(12):3440-9. doi: 10.1128/ Two New Species of Homalometron (Digenea: Apocreadiidae) AEM.00192-16. Print 2016 Jun 15 from Nearctic Freshwater Fundulids, Elucidation of the Life Cycle of H. cupuloris, and Molecular Phylogenetic Analysis of Some Doanh NP, Tu AL, Bui TD, Loan TH, Nonaka N, Horii Y, Blair D, Congeners. J Parasitol. 2016 Feb;102(1):94-104. doi: 10.1645/15- Nawa Y. Molecular and morphological variation of Paragonimus 862. Epub 2015 Nov 5. westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province. Parasitology. Fraser TA, Charleston M, Martin A, Polkinghorne A, Carver S. The 2016 Oct;143(12):1639-46. doi: 10.1017/S0031182016001219. emergence of sarcoptic mange in Australian wildlife: an unresolved Epub 2016 Jul 21. debate. Parasit Vectors. 2016 Jun 2;9(1):316. doi: 10.1186/s13071- 016-1578-2. Review. Doyle SR, Armoo S, Renz A, Taylor MJ, Osei-Atweneboana MY, Grant WN. Discrimination between Onchocerca volvulus and Gasser RB, Korhonen PK, Zhu XQ, Young ND. Harnessing the O. ochengi filarial larvae in Simulium damnosum (s.l.) and their Toxocara Genome to Underpin Toxocariasis Research and New distribution throughout central Ghana using a versatile high- Interventions. Adv Parasitol. 2016;91:87-110. doi: 10.1016/ resolution speciation assay. Doyle SR, Armoo S, Renz A, Taylor MJ, bs.apar.2015.12.001. Epub 2016 Feb 28. Review.

Page 85 Appendix 1

Ghorashi SA, Tavassoli M, Peters A, Shamsi S, Hajipour N. 26;5(2):184-91. doi: 10.1016/j.ijppaw.2016.05.002. eCollection Phylogenetic relationships among Linguatula serrata isolates from 2016 Aug. Review. Iran based on 18S rRNA and mitochondrial cox1 gene sequences. Acta Parasitol. 2016 Jan;61(1):195-200. Hillman AE, Yang R, Lymbery AJ, Thompson RC. Eimeria spp. infecting quenda (Isoodon obesulus) in the greater Perth region, Giannelli A, Kirkova Z, Abramo F, Latrofa MS, Campbell B, Zizzo Western Australia. Exp Parasitol. 2016 Nov;170:148-155. doi: N, Cantacessi C, Dantas-Torres F, Otranto D. Angiostrongylus 10.1016/j.exppara.2016.09.012. Epub 2016 Sep 28. chabaudi in felids: New findings and a review of the literature. Vet Parasitol. 2016 Sep 15;228:188-192. doi: 10.1016/j. Hing S, Currie A, Broomfield S,Keatley S, Jones K, Thompson RC, vetpar.2016.09.007. Epub 2016 Sep 11. Narayan E, Godfrey SS. Host stress physiology and Trypanosoma haemoparasite infection influence innate immunity in the woylie Gillett AK, Ploeg R, O’Donoghue PJ, Chapman PA, Webb RI, (Bettongia penicillata). Comp Immunol Microbiol Infect Dis. 2016 Flint M, Mills PC. Ultrastructural and Molecular Characterisation Jun;46:32-9. doi: 10.1016/j.cimid.2016.04.005. Epub 2016 Apr 14. of an Heterosporis-Like Microsporidian in Australian Sea Snakes (Hydrophiinae). PLoS One. 2016 Mar 23;11(3):e0150724. doi: Hobbs RP, Elliot AD. A new species of Potoroxyuris (Nematoda: 10.1371/journal.pone.0150724. eCollection 2016. Oxyuridae) from the woylie Bettongia penicillata (Marsupialia: Potoroidae) from southwestern Australia. Int J Parasitol Parasites Gofton AW, Doggett S, Ratchford A, Ryan U, Irwin P. Wildl. 2016 Jun 27;5(3):211-6. doi: 10.1016/j.ijppaw.2016.06.004. Phylogenetic characterisation of two novel Anaplasmataceae eCollection 2016 Dec. from Australian Ixodes holocyclus ticks: ‘Candidatus Neoehrlichia australis’ and ‘Candidatus Neoehrlichia arcana’. Int J Syst Hoyal Cuthill JF, Sewell KB, Cannon LR, Charleston MA, Lawler Evol Microbiol. 2016 Oct;66(10):4256-4261. doi: 10.1099/ S, Littlewood DT, Olson PD, Blair D. Australian spiny mountain ijsem.0.001344. Epub 2016 Jul 27. crayfish and their temnocephalan ectosymbionts: an ancient association on the edge of coextinction? Proc Biol Sci. 2016 May Gordon CA, McManus DP, Jones MK, Gray DJ, Gobert GN. The 25;283(1831). pii: 20160585. doi: 10.1098/rspb.2016.0585. Increase of Exotic Zoonotic Helminth Infections: The Impact of Urbanization, Climate Change and Globalization. Adv Parasitol. Hulst F, Kemp LF, Slapeta J. A new coccidian parasite of the boodie, 2016;91:311-97. doi: 10.1016/bs.apar.2015.12.002. Epub 2016 Bettongia lesueur (Mammalia: Marsupialia: Potoroidae), from Feb 27. Review. Australia. Folia Parasitol (Praha). 2016 Oct 20;63. pii: 2016.036. doi: 10.14411/fp.2016.036. Greay TL, Oskam CL, Gofton AW, Rees RL, Ryan UM, Irwin PJ. A survey of ticks (Acari: Ixodidae) of companion animals in Australia. Hunt VL, Grant W et al The genomic basis of parasitism in Parasit Vectors. 2016 May 10;9(1):207. doi: 10.1186/s13071-016- the Strongyloides clade of nematodes. Nat Genet. 2016 1480-y Mar;48(3):299-307. doi: 10.1038/ng.3495. Epub 2016 Feb 1.

Gulia-Nuss M, Barker SC et al Genomic insights into the Ixodes Huston DC, Cutmore SC, Cribb TH. The life-cycle scapularis tick vector of Lyme disease. Nat Commun. 2016 Feb of Gorgocephalus yaaji Bray & Cribb, 2005 (Digenea: 9;7:10507. doi: 10.1038/ncomms10507. Gorgocephalidae) with a review of the first intermediate hosts for the superfamily Lepocreadioidea Odhner, 1905. Syst Parasitol. Haddaway NR, Watson MJ. On the benefits of systematic reviews 2016 Sep;93(7):653-65. doi: 10.1007/s11230-016-9655-7. Epub for wildlife parasitology. Int J Parasitol Parasites Wildl. 2016 May 2016 Aug 13.

Page 86 Appendix 1

Hutson KS, Boxshall GA. Discovery of the male of the rare Nov;45:369-377. doi: 10.1016/j.meegid.2016.09.024. Epub 2016 caligiform copepod Kabataia Kazachenko, Korotaeva & Kurochkin, Oct 1. 1972 (Copepoda: Siphonostomatoida), with a reconsideration of its phylogenetic affinities. Zootaxa. 2016 Oct 11;4174(1):122-136. Leontovyč R, Young ND, Korhonen PK, Hall RS, Tan P, Mikeš doi: 10.11646/zootaxa.4174.1.9. L, Kašný M, Horák P, Gasser RB. Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Inpankaew T, Hii SF, Chimnoi W, Traub RJ. Canine vector-borne Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian pathogens in semi-domesticated dogs residing in northern Definitive Host. PLoS Negl Trop Dis. 2016 Feb 10;10(2):e0004406. Cambodia. Parasit Vectors. 2016 May 10;9(1):253. doi: 10.1186/ doi: 10.1371/journal.pntd.0004406. eCollection 2016 Feb. s13071-016-1552-z. Leung TL, Koprivnikar J. Nematode parasite diversity in birds: the Khan NH, Messenger LA, Wahid S, Sutherland CJ. Phylogenetic role of host ecology, life history and migration. J Anim Ecol. 2016 position of Leishmania isolates from Khyber Pakhtunkhwa province Nov;85(6):1471-1480. doi: 10.1111/1365-2656.12581. Epub 2016 of Pakistan. Exp Parasitol. 2016 Aug;167:61-6. doi: 10.1016/j. Sep 12. exppara.2016.05.006. Epub 2016 May 24. Liang D, Wang T, Rotgans BA, McManus DP, Cummins SF. Koehler AV, Haydon SR, Jex AR, Gasser RB. Is Cryptosporidium Ionotropic Receptors Identified within the Tentacle of the from the common wombat (Vombatus ursinus) a new species and Freshwater Snail Biomphalaria glabrata, an Intermediate Host of distinct from Cryptosporidium ubiquitum? Infect Genet Evol. 2016 Schistosoma mansoni. PLoS One. 2016 Jun 2;11(6):e0156380. doi: Oct;44:28-33. doi: 10.1016/j.meegid.2016.06.028. Epub 2016 Jun 10.1371/journal.pone.0156380. eCollection 2016. 14. Liang D, Zhao M, Wang T, McManus DP, Cummins SF. GPCR and IR Korneva JV, Kornienko SA, Jones MK. Fine structure of uterus and genes in Schistosoma mansoni miracidia. Parasit Vectors. 2016 Oct non-functioning paruterine organ in Orthoskrjabinia junlanae 26;9(1):563. (Cestoda, Cyclophyllidea). Parasitol Res. 2016 Jun;115(6):2449-57. doi: 10.1007/s00436-016-4997-2. Epub 2016 Mar 21. Loh SM, Gofton AW, Lo N, Gillett A, Ryan UM, Irwin PJ, Oskam CL. Kudlai O, Cribb TH, Cutmore SC. A new species of microphallid (Trematoda: Digenea) infecting a novel host family, the Novel Borrelia species detected in echidna ticks, Bothriocroton Muraenidae, on the northern Great Barrier Reef, Australia. Syst concolor, in Australia. Parasit Vectors. 2016 Jun 14;9(1):339. doi: Parasitol. 2016 Nov;93(9):863-876. Epub 2016 Oct 14. 10.1186/s13071-016-1627-x.

Le TH, Anh NT, Nguyen KT, Nguyen NT, Thuy do TT, Gasser RB. Ma G, Luo Y, Zhu H, Luo Y, Korhonen PK, Young ND, Gasser Toxocara malaysiensis infection in domestic cats in Vietnam--An RB, Zhou R. MicroRNAs of Toxocara canis and their predicted emerging zoonotic issue? Infect Genet Evol. 2016 Jan;37:94-8. doi: functional roles. Parasit Vectors. 2016 Apr 23;9:229. doi: 10.1186/ 10.1016/j.meegid.2015.11.009. Epub 2015 Nov 14. s13071-016-1508-3.

Le TH, Nguyen NT, Nguyen KT, Doan HT, Dung DT, Blair D. A Ma J, He JJ, Liu GH, Blair D, Liu LZ, Liu Y, Zhu XQ. Mitochondrial complete mitochondrial genome from Echinochasmus japonicus genome of Ogmocotyle sikae and implications for phylogenetic supports the elevation of Echinochasminae Odhner, 1910 to studies of the Notocotylidae trematodes. Infect Genet Evol. 2016 family rank (Trematoda: Platyhelminthes). Infect Genet Evol. 2016 Jan;37:208-14. doi: 10.1016/j.meegid.2015.11.018. Epub 2015

Page 87 Appendix 1

Nov 25. Nov 1;11(11):e0165918. doi: 10.1371/journal.pone.0165918. eCollection 2016 Maceda-Veiga A, Green AJ, Poulin R, Lagrue C. Body Condition Peaks at Intermediate Parasite Loads in the Common Nkouawa A, Haukisalmi V, Li T, Nakao M, Lavikainen A, Chen X, Bully Gobiomorphus cotidianus. PLoS One. 2016 Dec Henttonen H, Ito A. Cryptic diversity in hymenolepidid tapeworms 28;11(12):e0168992. doi: 10.1371/journal.pone.0168992. infecting humans. Parasitol Int. 2016 Apr;65(2):83-6. doi: eCollection 2016. 10.1016/j.parint.2015.10.009. Epub 2015 Oct 29.

MacLeod CD, Poulin R. Parasitic infection: a buffer against ocean Nolan MJ, Cantacessi C, Cutmore SC, Cribb TH, Miller TL. acidification? Biol Lett. 2016 May;12(5). pii: 20160007. doi: High-intensity cardiac infections of Phthinomita heinigerae n. 10.1098/rsbl.2016.0007. sp. (Digenea: Aporocotylidae) in the orangelined cardinalfish, Taeniamia fucata (Cantor), off Heron Island on the Great Barrier Maure F, Poulin R. Inequalities in body size among mermithid Reef. Parasitol Int. 2016 Oct;65(5 Pt A):371-7. doi: 10.1016/j. nematodes parasitizing earwigs. Parasitol Res. 2016 parint.2016.05.006. Epub 2016 May 14. Dec;115(12):4471-4475. Epub 2016 Aug 22. Pérez-Ponce DE León G, García-Varela M, Pinacho-Pinacho CD, Moore CV, Thompson RC, Jabbar A, Williams J, Rasiah K, Pallant L, Sereno-Uribe AL, Poulin R. Species delimitation in trematodes Koehler AP, Graham C, Weldhagen GF. Rare Human Infection with using DNA sequences: Middle-American Clinostomum as a case Pacific Broad Tapeworm Adenocephalus pacificus, Australia. Emerg study. Parasitology. 2016 Nov;143(13):1773-1789. Epub 2016 Aug Infect Dis. 2016 Aug;22(8):1510-2. doi: 10.3201/eid2208.160156 30.

Moravec F, Adlard R. Redescription of Rhabdochona papuanensis Pérez-Ponce de León G, Poulin R. Taxonomic distribution of cryptic (Nematoda: Thelazioidea), a parasite of rainbow fishes diversity among metazoans: not so homogeneous after all. Biol (Melanotaenia spp.); the first record of the species of Rhabdochona Lett. 2016 Aug;12(8). pii: 20160371. doi: 10.1098/rsbl.2016.0371. in Australia. Acta Parasitol. 2016 Dec 1;61(4):820-827. doi: 10.1515/ap-2016-0114. Pichelin S, Smales LR, Cribb TH. A review of the genus Sclerocollum Schmidt & Paperna, 1978 (Acanthocephala: Moravec F, Barton DP. New tissue-dwelling species of Philometra Cavisomidae) from rabbitfishes (Siganidae) in the Indian and Costa, 1845 and Philometroides Yamaguti, 1935 (Nematoda: Pacific Oceans. Syst Parasitol. 2016 Feb;93(2):101-14. doi: Philometridae) from marine perciform fishes off the northern coast 10.1007/s11230-015-9609-5. Epub 2016 Jan 20. of Australia. Syst Parasitol. 2016 Sep;93(7):623-37. doi: 10.1007/ s11230-016-9657-5. Epub 2016 Aug 13. Pomaznoy MY, Logacheva MD, Young ND, Penin AA, Ershov NI, Katokhin AV, Mordvinov VA. Whole transcriptome profiling Nguyen C, Koh WL, Casteriano A, Beijerink N, Godfrey C, Brown of adult and infective stages of the trematode Opisthorchis G, Emery D, Šlapeta J. Mosquito-borne heartworm Dirofilaria felineus. Parasitol Int. 2016 Feb;65(1):12-9. doi: 10.1016/j. immitis in dogs from Australia. Parasit Vectors. 2016 Oct parint.2015.09.002. Epub 2015 Sep 9. 7;9(1):535. Portas T.M., Cunningham R.B., Spratt D., Devlin J. Holz P., Niebuhr CN, Poulin R, Tompkins DM. Is Avian Malaria Playing Batson W., Owens J., Manning A.D. (2016) Beyond morbidity a Role in Native Bird Declines in New Zealand? Testing and mortality in reintroduction programmes: changing health Hypotheses along an Elevational Gradient. PLoS One. 2016 parameters in reintroduced eastern bettongs, Bettongia

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gaimardi.. Oryx published online 28 March 2016, 10pp. DOI: Russell TL, Beebe NW, Bugoro H, Apairamo A, Chow WK, Cooper 10.1017/S0030605315001283 RD, Collins FH, Lobo NF, Burkot TR. Frequent blood feeding enables insecticide-treated nets to reduce transmission by Poulin R, Besson AA, Morin MB, Randhawa HS. Missing links: mosquitoes that bite predominately outdoors. Malar J. 2016 Mar testing the completeness of host-parasite checklists. Parasitology. 10;15:156. doi: 10.1186/s12936-016-1195-8. 2016 Jan;143(1):114-22. doi: 10.1017/S0031182015001559. Epub 2015 Nov 9. Russell TL, Beebe NW, Bugoro H, Apairamo A, Collins FH, Cooper RD, Lobo NF, Burkot TR. Anopheles farauti is a homogeneous Poulin R, Presswell B. Taxonomic Quality of Species Descriptions population that blood feeds early and outdoors in the Solomon Varies over Time and with the Number of Authors, but Unevenly Islands. Malar J. 2016 Mar 9;15:151. doi: 10.1186/s12936-016- among Parasitic Taxa. Syst Biol. 2016 Nov;65(6):1107-1116. Epub 1194-9. 2016 Jun 10. Russell TL, Beebe NW, Bugoro H, Apairamo A, Cooper RD, Collins Reid SA, McKenzie J, Woldeyohannes SM. One Health research FH, Lobo NF, Burkot TR. Determinants of host feeding success by and training in Australia and New Zealand. Infect Ecol Epidemiol. Anopheles farauti. Malar J. 2016 Mar 10;15:152. doi: 10.1186/ 2016 Nov 29;6:33799. doi: 10.3402/iee.v6.33799. eCollection s12936-016-1168-y. 2016. Review. Russell TL, Burkot TR, Bugoro H, Apairamo A, Beebe NW, Reverter M, Cutmore SC, Bray R, Cribb TH, Sasal P. Gill Chow WK, Cooper RD, Collins FH, Lobo NF. Larval habitats of monogenean communities (Platyhelminthes, Monogenea, the Anopheles farauti and Anopheles lungae complexes in the Dactylogyridae) of butterflyfishes from tropical Indo-West Pacific Solomon Islands. Malar J. 2016 Mar 15;15:164. doi: 10.1186/ Islands. Parasitology. 2016 Oct;143(12):1580-91. doi: 10.1017/ s12936-016-1196-7. S0031182016001463. Epub 2016 Aug 30. Schiller SE, Webster KN, Power M. Detection of Cryptosporidium Richardson MF, Sherman CD, Lee RS, Bott NJ, Hirst AJ. Multiple hominis and novel Cryptosporidium bat genotypes in wild and dispersal vectors drive range expansion in an invasive marine captive Pteropus hosts in Australia. Infect Genet Evol. 2016 species. Mol Ecol. 2016 Oct;25(20):5001-5014. doi: 10.1111/ Oct;44:254-60. doi: 10.1016/j.meegid.2016.07.002. Epub 2016 mec.13817. Epub 2016 Sep 12. Jul 4.

Ross E, Blair D, Guerrero-Hernández C, Sánchez Alvarado A. Scholz T, Besprozvannykh VV, Boutorina TE, Choudhury A, Cribb Comparative and Transcriptome Analyses Uncover Key Aspects TH, Ermolenko AV, Faltýnková A, Shedko MB, Shimazu T, Smit NJ. of Coding- and Long Noncoding RNAs in Flatworm Mitochondrial Trematode diversity in freshwater fishes of the Globe I: ‘Old World’. Genomes. G3 (Bethesda). 2016 May 3;6(5):1191-200. doi: Syst Parasitol. 2016 Mar;93(3):257-69. doi: 10.1007/s11230-016- 10.1534/g3.116.028175. 9630-3. Epub 2016 Feb 22. Review.

Ross KE, Bradbury RS, Garrard TA, O’Donahoo FJ, Shield JM, Schrandt MN, Andres MJ, Powers SP, Overstreet RM. Novel Page W, Miller A, Robertson G, Judd JA, Speare R. The National Infection Site and Ecology of Cryptic Didymocystis sp. (Trematoda) Strongyloides Working Group in Australia 10 workshops on: in the Fish Scomberomorus maculatus. J Parasitol. 2016 commendations and recommendations. Aust N Z J Public Health. Jun;102(3):297-305. doi: 10.1645/15-772. Epub 2016 Mar 16. 2016 Dec 13. doi: 10.1111/1753-6405.12611 Shuttleworth M, Jabbar A, Beveridge I. Description and

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molecular characterisation of Cloacina johnstoni sp. nov. Dingoes (Canis dingo Meyer, 1793) continue to be an important (Nematoda: Strongyloidea) from the wallaroo, Macropus reservoir host of Dirofilaria immitis in low density housing areas robustus (Marsupialia: Macropodidae) and relationships with in Australia. Vet Parasitol. 2016 Jan 15;215:6-10. doi: 10.1016/j. the synhospitalic species C. macropodis. Parasitol Res. 2016 vetpar.2015.10.020. Epub 2015 Oct 27. Sep;115(9):3357-63. doi: 10.1007/s00436-016-5096-0. Epub 2016 May 12. Speare R, Bradbury RS, Croese J. A Case of Ancylostoma ceylanicum Infection Occurring in an Australian Soldier Returned Shuttleworth MA, Beveridge I, Koehler AV, Gasser RB, from Solomon Islands. Korean J Parasitol. 2016 Aug;54(4):533-6. Jabbar A. Molecular characterization of species of Cloacina doi: 10.3347/kjp.2016.54.4.533. Epub 2016 Aug 31. (Strongyloidea: Cloacininae) from the common wallaroo, Macropus robustus (Marsupialia: Macropodidae) in Australia. Infec t Genet Spratt D.M. Zoonotic tissue parasites of Australian wildlife. Evol. 2016 Oct;44:245-53. doi: 10.1016/j.meegid.2016.06.037. Microbiology Australia (Under the Microscope) February 2016 Epub 2016 Jul 15. 10.1071/MA16005 pp. 7-9

Sidabalok CM, Bruce NL. Redescription of three cirolanid isopods Spratt DM, Beveridge I. Helminth parasites of Australasian (Crustacea: Peracarida) from Indonesia. Zootaxa. 2016 May monotremes and marsupials. Zootaxa. 2016 Jun 15;4123(1):1-198. 24;4114(3):277-90. doi: 10.11646/zootaxa.4114.3.4. doi: 10.11646/zootaxa.4123.1.1.

Šlapeta J, Morin-Adeline V, Thompson P, McDonell D, Shiels M, Stephens PR, Altizer S, Smith KF, Alonso Aguirre A, Brown JH, Gilchrist K, Votýpka J, Vogelnest L. Intercontinental distribution Budischak SA, Byers JE, Dallas TA, Jonathan Davies T, Drake JM, of a new trypanosome species from Australian endemic Ezenwa VO, Farrell MJ, Gittleman JL, Han BA, Huang S, Hutchinson Regent Honeyeater (Anthochaera phrygia). Parasitology. 2016 RA, Johnson P, Nunn CL, Onstad D, Park A, Vazquez-Prokopec GM, Jul;143(8):1012-25. doi: 10.1017/S0031182016000329. Epub Schmidt JP, Poulin R. The macroecology of infectious diseases: a 2016 Mar 22. new perspective on global-scale drivers of pathogen distributions and impacts. Ecol Lett. 2016 Sep;19(9):1159-71. doi: 10.1111/ Smales LR The gastrointestinal helminths of Rattus giluwensis ele.12644. Epub 2016 Jun 28. Review. and Rattus novaeguineae (Rodentia: Muridae) with descriptions of two new species (Nematoda) from Papua New Guinea. Stumbo AD, Poulin R. Possible mechanism of host manipulation Comparative Parasitology 83: 162-172. resulting from a diel behaviour pattern of eye-dwelling parasites? Parasitology. 2016 Sep;143(10):1261-7. doi: 10.1017/ Smales LR The gastrointestinal helminths of Rattus niobe S0031182016000810. Epub 2016 May 24. (Rodentia: Muridae) with descriptions of two new genera and three new species (Nematoda) from Papua New Guinea and Suwannatrai K, Suwannatrai A, Tabsripair P, Welbat JU, Papua Indonesia. Zootaxa 4117: 183-197 Tangkawattana S, Cantacessi C, Mulvenna J, Tesana S, Loukas A, Sotillo J. Differential Protein Expression in the Hemolymph Smales LR, Al-Hasson HAH, Al-Niaeem KS, Al-Azizz S. A new of Bithynia siamensis goniomphalos Infected with Opisthorchis species of Neorhadinorhynchus (Acanthocephala: Cavisomidae) viverrini. PLoS Negl Trop Dis. 2016 Nov 28;10(11):e0005104. doi: from Platax teira (Ephippidae) from Iraqi marine waters. 10.1371/journal.pntd.0005104. eCollection 2016 Nov. Transactions of the Royal Society of South Australia 140: Vaughan DB, Chisholm LA, Hansen H. Electrocotyle whittingtoni Smout FA, Skerratt LF, Butler JR, Johnson CN, Congdon BC. n. gen., n. sp. (Monogenea: Monocotylidae: Heterocotylinae) from

Page 90 Appendix 1

the gills of a captive onefin electric ray, Narke capensis (Narkidae) Yang R, Brice B, Ryan U. Morphological and molecular at Two Oceans Aquarium, Cape Town, South Africa. Parasitol Res. characterization of Eimeria purpureicephali n. sp. 2016 Sep;115(9):3575-84. doi: 10.1007/s00436-016-5123-1. (Apicomplexa:Eimeriidae) in a red-capped parrot Epub 2016 Jun 1. (Purpureicephalus spurius, Kuhl, 1820) in Western Australia. Int J Parasitol Parasites Wildl. 2016 Jan 12;5(1):34-9. doi: 10.1016/j. Vermuelen, E.T., Power, M.L., Nipperess, D.A., Beveridge, ijppaw.2016.01.003. eCollection 2016 Apr. I. & Eldridge, M.D.B. Biodiversity of parasite assemblages in the genus Petrogale and its relation to the phylogeny and Yang R, Brice B, Ryan U. Morphological and molecular biogeography of their hosts. Australian Journal of Zoology 64: characterization of Isospora neochmiae n. sp. in a captive-bred red- 61-80. browed finch (Neochmia temporalis) (Latham, 1802). Exp Parasitol. 2016 Jul;166:181-8. doi: 10.1016/j.exppara.2016.04.011. Epub Vermeulen ET, Lott MJ, Eldridge MD, Power ML. Evaluation 2016 Apr 21. of next generation sequencing for the analysis of Eimeria communities in wildlife. J Microbiol Methods. 2016 May;124:1-9. Yang R, Dorrestein GM, Ryan U. Molecular characterisation of a doi: 10.1016/j.mimet.2016.02.018. Epub 2016 Mar 2. disseminated Cryptosporidium infection in a Koi carp (Cyprinus carpio). Vet Parasitol. 2016 Aug 15;226:53-6. doi: 10.1016/j. Wang T, Zhao M, Rotgans BA, Strong A, Liang D, Ni G, Limpanont vetpar.2016.06.027. Epub 2016 Jun 21. Y, Ramasoota P, McManus DP, Cummins SF. Proteomic Analysis of the Schistosoma mansoni Miracidium. PLoS One. 2016 Jan Yong RQ, Cutmore SC, Miller TL, Wee NQ, Cribb TH. A complex 22;11(1):e0147247. doi: 10.1371/journal.pone.0147247. of Cardicola Short, 1953 (Digenea: Aporocotylidae) species eCollection 2016. infecting the milkfish Chanos chanos Forsskål (Gonorynchiformes), with descriptions of two new species. Syst Parasitol. 2016 Yang R, Brice B, Elloit A, Ryan U. Morphological and Nov;93(9):831-846. Epub 2016 Oct 14. molecular characterization of Eimeria labbeana-like (Apicomplexa:Eimeriidae) in a domestic pigeon (Columba Yong, RQ, Cutmore SC, Bray RA, Miller TL, Semarariana IWY, livia domestica, Gmelin, 1789) in Australia. Exp Parasitol. 2016 Palm HW, Cribb TH Three new species of blood flukes (Digenea: Jul;166:124-30. doi: 10.1016/j.exppara.2016.04.009. Epub 2016 Aporocotylidae) infecting pufferfishes (Teleostei: Tetraodontidae) Apr 12. from off Bali, Indonesia. Parasitology international, 65, 432–443.

Yang R, Brice B, Jian F, Ryan U. Morphological and molecular characterization of Isospora manorinae n. sp. in a yellow-throated miner (Manorina flavigula wayensis) (Gould, 1840). Exp Parasitol. 2016 Apr;163:16-23. doi: 10.1016/j.exppara.2016.01.013. Epub 2016 Jan 26.

Yang R, Brice B, Ryan U. Morphological and molecular characterization of Choleoeimeria pogonae n. sp. coccidian parasite (Apicomplexa: Eimeriidae, 1989, Paperna and Landsberg) in a western bearded dragon (Pogona minor minor). Exp Parasitol. 2016 Jan;160:11-6. doi: 10.1016/j.exppara.2015.11.001. Epub 2015 Nov 14.

Page 91 Appendix 2: Research grants awarded to ASP Members in 2016

IN 2016, ASP MEMBERS SECURED and management protocols for tick-borne disease in Australia. OVER $33 MILLION IN NEW RESEARCH GRANTS AND FELLOWSHIPS. ARC Discovery Grants

Institution names in the list below refer to the Australian Administering Institution, not necessarily the home Philip Andrews, Lukasz Kedzierski, Hongzhe Sun and Michael institution of individual Researchers) Mehring (Monash University) – to develop new anti-leishmanials.

ARC Future Fellowships ARC Linkage Grants Rowena Martin (ANU) – to continue her efforts to understand Professor Terence Spithill; Dr Travis Beddoe; Dr Robert antimalarial drug action and resistance. Dempster; Dr Glenn Anderson (Virbac Australia Pty Ltd and La Trobe University) This project aims to optimise the formulation of novel fluke vaccine antigens by constructing combination hybrid NHMRC Senior Principal Research Fellowships recombinant antigens and using a protein adjuvant to improve immunogenicity, and test new antigens expressed in young flukes Alex Loukas (JCU) – an NHMRC Senior Principal Research as vaccines and evaluate their ability to synergise with hybrid Fellowship to continue his work on helminth immunobiology and vaccines. Fasciola (fluke) infections cause serious economic losses vaccine development. to livestock production and fluke drug resistance threatens control, so new therapies such as a vaccine are needed. These vaccines Alan Cowman (WEHI) – an NHMRC Senior Principal Research should be evaluated in cattle trials. The major outcome plan is Fellowship to continue his research on the biology of the malaria validation of hybrid antigens for commercial vaccine development parasite. for fluke control in cattle, leading to more sustainable beef and milk production in Australia. NHMRC Principal Research Fellowships Professor Peter Irwin; Professor Una Ryan; Dr Charlotte Oskam; Dr Liisa Ahlstrom; Associate Professor Peter Banks; John Baell (Monash University) – to develop new anti-parasitic Professor Roy Hall; Dr Sonja Hall-Mendelin; Dr Bettina drugs. Schunack (Bayer Australia Ltd, Bayer Healthcare, Queensland Health and Murdoch University) This project aims to determine the bacterial, protozoal and viral biodiversity in wildlife ticks and NHMRC Career Development Fellowships their native mammal hosts, and provide new information about the biology and transmission dynamics of these microorganisms and Aaron Jex (The Walter and Eliza Hall Institute of Medical Research) their potential to cause disease in wildlife, domesticated animals R.D. Wright Biomedical Fellowship, Systems biology of neglected and humans. Anticipated outcomes are improved diagnostic tests parasites

Page 92 Appendix 2

NHMRC Early Career Research Fellowships University of Notre Dame Australia), Andrew Steer (Murdoch Childrens Research Institute), Roz Walker (University of Western Robert Summers (ANU) – to carry out research to suppress Australia), Julie Marsh (Telethon Kids Institute, Subiaco WA), antimalarial drug resistance. Raymond Christophers, Skin disease control in remote Aboriginal children: translating evidence into practice with a cluster Pasi Korbonen (The University of Melbourne) – to harness systems randomised, stepped wedge trial biology to tackle neglected tropical diseases. Brendan Crabb (Burnet Institute), Travis Beddoe (La Trobe University), Tania de Koning-Ward (Deakin University), Paul NHMRC Project Grants Gilson (Burnet Institute), Functional resolution of PTEX, the exporter of virulence factors in malaria parasites Alan Cowman, Justin Boddey. Wilson Wong and Anthony Hodder (all Walter and Eliza Hall Institute of Medical Research), Christopher MacRaild (Monash University), Raymond Norton Effector export in P. falciparum infected human erythrocytes (Monash University), Jonathan Richards (Burnet Institute), Enhancing the immune response to disordered malaria antigens Andrew Steer (Murdoch Childrens Research Institute), Margot Whitfeld (St Vincents and Mater Health Sydney), Tibor Schuster Laurens Manning (University of Western Australia), Timothy (Murdoch Childrens Research Institute), Titus Nasi (National Davis (University of Western Australia), Brioni Moore (University Referral Hospital, Honiara), Oliver Sokana (Ministry of Health of Western Australia), Moses Laman (Papua New Guinea Institute and Medical Services Solomon Islands), Ross Andrews (Menzies of Medical Research, Goroka, Papua New Guinea), Kevin Batty School of Health Research), Lucia Romani (The Kirby Institute for (Curtin University of Technology), Sam Salman (University of infection and immunity in society), Michael Marks (London School Western Australia), Leanne Robinson (The Walter and Eliza Hall of Hygiene and Tropical Medicine), Daniel Engelman (University Institute of Medical Research), Enhancing clinical management of of Melbourne - Centre for International Child Health), Cluster paediatric malaria in endemic areas with transmission of multiple randomised trial comparing one versus two doses of ivermectin for Plasmodium species mass drug administration to control scabies Malcolm Jones (The University of Queensland), A new animal Andrew Steer (Murdoch Childrens Research Institute), Margot model for genitourinary schistosomiasis Whitfeld (St Vincents and Mater Health Sydney), Handan Wand (University of New South Wales), Mike Kama (Fiji Centre Malcolm McConville (University of Melbourne), Christopher for Communicable Disease Control, Mataika House, Suva, Fiji), Tonkin (The Walter and Eliza Hall Institute of Medical Research), Joseph Kado (Fiji Ministry of Health), Ross Andrews (Menzies Vernon Carruthers (University of Michigan Health System), School of Health Research), Lucia Romani (The Kirby Institute for Targeting acute and chronic toxoplasmosis infection and immunity in society), Natalie Carvalho (University of Melbourne), Does mass drug administration for scabies result Michael Duffy (University of Melbourne), Tania de Koning- in control of serious bacterial complications? A proof of concept Ward (Deakin University), Stefan Knapp (Goethe University towards global elimination Frankfurt), The role of novel and essential bromodomain proteins in coordinating malaria parasite gene regulation and their potential Asha Bowen (Telethon Kids Institute, Subiaco WA), Jonathan as anti-malarial targets Carapetis (Telethon Kids Institute, Subiaco WA), Steven Tong (Menzies School of Health Research), Julianne Coffin (The Michelle Boyle (Burnet Institute), T-follicular helper cell subsets

Page 93 Appendix 2

that induce protective anti- Plasmodium falciparum antibodies Vicky Avery Griffith University Research Infrastructure Project (GURIP): Imaging and Quantitative Cell Analysis Facility. Nicholas Smith (Western Sydney University), Adrian Hehl (University of Zurich), Peter Deplazes (University of Zurich), A Amy Jones CASS Travel Grant and ASM travel award to attend the transmission-blocking vaccine to prevent toxoplasmosis ASM (American Society of Microbiology) Microbe Meeting held June 16th-20th 2016, Boston, USA to give an oral presentation. Rowena Martin (Australian National University), Adele Lehane (Australian National University), Determining the mechanistic basis Amy Jones Theo Murphy (Australia) Fund, (administered by the UK of the patterns of inverse drug susceptibility induced by two key Royal Society) to participate in the 2016 Theo Murphy High Flyers drug resistance proteins of the malaria parasite Think Tank – An interdisciplinary approach to living in a risky world (Australian Academy of Science) held on 20-22 July in Canberra Scott Mueller (University of Melbourne), Immune surveillance of the CNS during malaria infection Melissa Sykes and Bilal Zulfiqar Tony B. Academic Travel award covering flights and accommodation to present a poster at the Stephan Karl (The Walter and Eliza Hall Institute of Medical SLAS2017 6th Annual Conference and Exhibition, February 4-8, Research), Istvan Kezsmarki (Budapest University of Techology 2017 in Washington, DC and Economics), Moses Laman (Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea), Malcolm Jones Bilal Zulfaqir Griffith University International Experience Incentive (The University of Queensland), Peter Metaxas (University of Scheme (IEIS) Travel Grant to be used towards his planned visit to Western Australia), Field-based evaluation of a novel magneto- Prof Louis Maes' lab in Belgium in 2017. optical technique to diagnose malaria James McCarthy (QIMR Berghofer Medical Research Institute) Timothy Davis (University of Western Australia), Brioni Moore (University of Western Australia), Moses Laman (Papua New • Bill and Melinda Gates Foundation Grand Challenges – New Guinea Institute of Medical Research, Goroka, Papua New Guinea), Interventions for Global Health: Novel Sensor and Biomarkers Kevin Batty (Curtin University of Technology), Laurens Manning for Diagnosis of Malaria Using Human Breath (University of Western Australia), Sam Salman (University of Western Australia), A study of artemisinin combination therapy • NHMRC Northern Australia Tropical Disease Collaborative given at delivery to prevent postpartum malaria and to young Research Progamme 1131932 Improving Health Outcomes in infants to treat uncomplicated malaria. the Tropical North: A Multidisciplinary Collaboration

• Wellcome Trust Translation Fund Award An innovative Other research grants antimalarial treatment – Proof of Efficacy in Malaria Human Challenge Model ASP members have reported the following additional funding. • PATH-MVI Controlled Human Malaria Infection Transmission Vicky Avery and Sandra Duffy Griffith University's Nature (CHMI Transmission) Incentive Scheme and $2500, for the contribution made to the following Nature article in 2016; Diversity-oriented synthesis yields • PATH Biomarkers for Malaria Elimination” “BIOME” novel multistage antimalarial inhibitors. Plasmodium Vivax (“Pv’)

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• PATH Plasmodium Falciparum (“Pf”) Histidine-Rich Protein Geoff McFadden (University of Melbourne) McCoy Foundation 2 (“HRP2”) Introduction and decay model to define HRP2 Grant for “Wildlife Pathogen Biobank” dynamics and Pf detection over time Michael Duffy (University of Melbourne) in partnership with • Medicines for Malaria Venture A proof-of-concept study to McGill University, the Medicines for Malaria Venture, the Structural assess the effect of (+)-SJ000557733 (SJ733) against early Genomics Consortium at University of Toronto and the Drugs for Plasmodium falciparum blood stage infection in healthy Neglected Diseases initiative, Global Health Innovative Technology participants Fund grant for, “Targeting bromodomains for novel anti-parasitic mechanisms of action for malaria, Chagas disease, leishmaniasis • Foundation for Innovative New Diagnostics (FIND) P. and cryptosporidiosis.” falciparum hrp2 sequencing project Ala Lew and Peter James (University of Queensland) Meat & • Medicines for Malaria Venture A single centre, two-part, Livestock Australia Donor Company (MDC) funding, to, for ‘Cattle Phase I study to investigate the safety, tolerability and tick and Buffalo fly host genetics, susceptibility to buffalo fly lesions pharmacokinetic profile of ascending oral doses of and biomarkers for resistance’. MMV390048 and its antimalarial activity against Plasmodium falciparum in healthy adult subjects Ala Lew (University of Queensland) Advance Queensland Innovation Partnership grant for ‘Improving the identification of • Medicines for Malaria Venture A Phase Ib experimental study tick resistance in beef cattle’, to, and collaborators. to assess the in vivo safety and response to chloroquine of Plasmodium vivax isolate HMPBS02-Pv in healthy participants with induced blood stage malaria infection

Page 95 Appendix 3: ASP Member Survey 2016 IN 2016 THE ASP SURVEYED MEMBERS Investigating models of governance and operation TO FIND OUT WHAT THEY THOUGHT for the Australian Society for Parasitology in a ABOUT GOVERNANCE, LEADERSHIP changing economic and social environment. AND OPERATIONS OF THE AUSTRALIAN Good corporate governance is essential for the smooth and SOCIETY FOR PARASITOLOGY TO HELP efficient financial and operational running of an organisation and to protect the rights of its leaders. This study analysed PLAN FOR THE FUTURE. THE FULL the governance and operations of the Australian Society for REPORT CAN BE DOWNLOADED FROM Parasitology (ASP) within the context of a changing social and THE MEMBERS RESOURCES SECTION OF economic environment. 156 participants were surveyed for their opinion about the governance and operations of the ASP and 20 THE ASP WILDAPRICOT MEMBERSHIP members of the ASP leadership team participated in telephone WEBSITE, SEE “APPENDIX 3 FOR 2016 interviews to identify the current governance and operational systems of the ASP. All participants were asked to consider how the ASP ETM & AGM DRAFT MINUTES…” ASP might operate in the future. HTTP://ASP.WILDAPRICOT.ORG/ MEMBERRESOURCES The research questions were: What should the Constitution of the Australian Society for Parasitology look like?

What is the best governance model for the Australian Society for Parasitology within the framework of the mission, vision and values identified in Constitution?

What are the possibilities for the Australian Society for Parasitology

Fig 1. ASP Operations

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in the future? Summary of key findings

External factors have affected the macro environment of the ASP, Survey participants (n=139) represented both male and female with effects on science research funding and job opportunities for ASP members equally, reflecting the ratio of males to females in scientists in Australia. These forces for change have made the ASP the whole of the ASP. In contrast, the gender ratio for interview consider how they might need to adapt in the future. participants (n=20), representing the ASP leadership team, had more males, (75%), than females, (25%), reflecting the current The aim of this research was to determine the best model for gender ratio of the ASP leadership team. ASP members from a governance and operation of the ASP to enable the Society to good spread of institutions and locations participated in the survey. continue to grow and develop within the constraints of a changing economic and social environment. 95% of participants agreed or strongly agreed with the ASP Object as stated in the ASP Constitution (2014): “The Society ASP Operations fosters association of persons interested in parasitology, fosters establishment and proper curation of collections of Australian The Operations of the ASP (Figure 1) can be analysed as a parasites, and, by facilitating intercourse and discussion, promotes transformation process where inputs of material, management, investigation and advances knowledge of parasitology”. Some labour and capital are identified, they go through a transformation commented that there were aspects missing including “outreach”, process and are turned into outputs, goods and services. ASP “international” and “student support”, and a few noted with Operations analysis showed that the ASP administered and surprise the inclusion of “curation of collections”. funded grants, professional development courses and networking opportunities for its members, it has frequently and regularly Participants thought that using a “voting-based method of organised large, international conferences, and supported decision making where the group agrees to a portion of votes and numerous public education and engagement initiatives financially the majority of votes wins” was used most frequently when the ASP and through in-kind contributions. leadership team were making decisions.

The ASP relished in giving opportunities for future generations of parasitologists, they were passionate about the discipline and ensuring the vibrancy and future of the Society. It was important to

Fig 2. Survey participant response to "What do you think the ASP mission and vision is?", themed, n=136

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identify what motivated participants to be an active ASP member “mapping the tasks and understanding the best way to do those to make sure that that motivation continued in the future. based on a streamlined and modernized structure” is essential. The future threats highlighted a combination of external factors Institutional “support” (or the lack thereof) for ASP activities was that the ASP have little influence over and internal factors that clearly an area that many participants felt strongly about; however they have full control over and the AGM is the ideal forum to have how to address that issue was not clear cut. Institutional support is a discussion and form a working group to feed into the strategic necessary to keep people in the discipline of parasitology. Some felt planning process. that the ASP needed to act as a lobby group and this could include lobbying within their own institution. Implicit in many comments Blue sky thinking around future income sources for the ASP was the thought that the status of the ASP as an independent and revealed that participants were not convinced this was necessary objective voice is one of the society's strengths that the ASP should or ideologically right because it doesn’t sit well with the culture not align with any one institution. and values of the Society. However, this might not apply to concepts like “crowd-funding”, “sponsorship”, “endowments” and Considering the future of the ASP and how to ensure that the “philanthropy” and are ideas worth pursuing as part of the future transformation process happens efficiently so that the outputs are planning for the ASP along with the concept of “clever spending”. more valuable than the inputs was asked through several questions The organisational culture of the ASP was revealed as a culture around threats to the ongoing stability of the science workforce. that enabled members to take risks, be fully engaged if they In terms of what happens during the transformation process desired, and a culture that has resulted in some highly creative

Figure 3. Survey participant "Motivation to be an active ASP member", categorised into 15 themes, n=102

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organisational outputs which has helped to make the ASP a rich The top ranked themes were “Research support”, “Outreach” and and vibrant society. “Network”.

ASP Constitution Survey and interview participants when asked to identify their motivation to be an active ASP member were united in “Early Survey participants gave eloquent interpretations of their version Career Researcher support” and a “collegial parasitology of the ASP mission and vision, two are highlighted here and all have community” as main themes (Figure 3 & 4). They gave the been coded into 15 themes (Figure 2). following comments:

“To promote, champion and advance the field of parasitology on a “Maintain contact with likeminded colleagues; maintain global scale” knowledge in parasitology; support early career researchers and students” “To promote the teaching and research of parasitology in Australia and worldwide. Support and promotion of young researchers and “Love of parasitology, desire to be part of the parasitology excellence in research. Education of, and communication to, the community, and want to support other members.” public about parasitology. Collection, curation and documentation of parasites for which there is little information. Promotion and “I am less active since retirement, but I love the organization, its fostering of international collaboration.” spirit and its members”

Fig 4. Interview participant "Motivation to be an active ASP member", themed, n=20

Page 99 “It is the only society that solely focuses on parasites and is one of views to be heard and accepted that a “majority vote” rules, also the society's that is most relevant to my research.” outlined in the ASP Constitution (2014).

“The collegiality and the opportunities the society creates for the “.. there have been hard moments in the past surrounding financial next generations of parasitologists.” issues, but other than that the future decisions have been most important and satisfying..” “The ASP promotes the science and training and mentoring students, we need to do this well if we want parasitology to thrive.” “..opportunities for the next generations of parasitologists that it ensured the vibrancy and future of the Society.” “Passionate about the discipline at large, thoroughly enjoy everything the ASP offers particularly the meetings,” Income sources

Leadership Survey and Interview participants were asked to do some “blue sky” thinking around potential future income sources for the When asked about the best decision they were involved in and ASP. Survey participants (Figure 5) were not sure it was necessary why Interview participants talked about the “financial support of (18%) but several thought of “crowd-funding”, “sponsorship”, initiatives”, “support for the CIP course” and described being part “courses” and “events”. Interview participants gave the following of “democratic decision making processes that made them feel suggestions (Figure 31) “corporate support or sponsorship”, good”. A majority of Interview participants felt the decision making profit making “courses”, “endowments” and “philanthropy”. One process that the ASP Council and Executive used enabled their suggested “Industry membership could be looked at to make it Fig.5. Survey participant suggestions for "future ways the ASP might generate more valuable.” Participants didn’t necessarily agree that the ASP income", categorised into the top 10 themes, n=81 needed to diversify its income, two comments from participants “the bigger issue is clever spending” and “I think that it does not need to Philanthrophy, find new ways to generate income, it could look investments, 12, 9% at its expenditure side if there is concern about Consultancy, 3, 2% balancing its considerable budget” identifies Crowd funding, 32, 25% Conferences, 4, 3% a different way of looking at increasing income for the ASP. Membership, 5, 4%

Donations/Fundraising/ Conclusion Bequests, 6, 5% One final comment highlighted the importance Merchandise, 7, 5% of maintaining the organisational culture of the ASP;

Public Events/Public Unsure/Uneccessary, 23, “The organisational culture of the ASP is such Education, 9, 7% 18% that individuals are allowed to take risks, they are engaged and they produce some really

Courses/Training, 14, creative activities that make the ASP such a 11% Sponsorship, 14, 11% vibrant society.”

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This study highlighted the importance of ASP members feeling 3) Discuss with ASP membership workforce employed to deliver like they own part of the society. Overall ASP members were ASP Operations, whether this is desirable and sustainable long happy with the ASP Operations, the governance and mission and term to support the work of the ASP volunteers to benefit the vision statements were congruent. Comments from participants Society and the discipline of parasitology. and information from other sources all resonated with principles that promote good governance and the following changes are Any proposed interventions need to have ASP membership recommended: support before being delivered and the change management process will be evaluated to enable the ASP to be a learning 1) Changes to the Constitution organisation within a changing macro-environment. a) Make the election processes for Council explicit in the Core competencies of organisations are hard to imitate and constitution with a page on the ASP website dedicated to achieved through people; with the right combination of people explaining how to nominate someone for Council, how it will be and communication channels the ASP could, as a team, contribute decided who is elected. more than each individual and this has been seen with the success of ASP Operational Outputs. To continue to flourish in b) Consider the representation of ASP members on Council, a constantly changing macro-environment the ASP needs to whether a student representative can be included. maintain competitive advantage as a learning organisation. Being able to lead change and deal with unplanned change are the core c) Assess voting status of Council and effect on decision making elements of learning organisations. With a focus on people with a process. shared vision; thoughtful communication; using systems thinking and continually challenging processes and taking risks the ASP has 2) Discuss with ASP membership whether the ASP should try to shown itself to being a learning organisation. make profit from some of their activities and, therefore, increase income, and the ways this could be achieved.

Page 101 Australian Society for Parasitology Inc. ABN 65 979 686 445

PO Box 60 Royal Brisbane Hospital QLD, Australia, 4029

Web: www.parasite.org.au Email: [email protected] Tel: +61 7 42321311