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PTPRE Sirna Set I PTPRE Sirna Set I Catalog # Aliquot Size P52-911B-05 3 x 5 nmol P52-911B-20 3 x 20 nmol P52-911B-50 3 x 50 nmol PTPRE siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P52-911B Lot # Z2085-30 Specificity Formulation PTPRE siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPRE expression. shipped at room temperature. Product Description Reconstitution Protocol PTPRE siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPRE mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is BC050062. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot PTPE, P-PTP-epsilon, HPTPE, R-PTP-EPSILON, RP11-380J17.1, 1x stock solutions for storage. DKFZp313F1310, FLJ57799, FLJ58245 Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the PTPRA (LRP), Active P48-21G lyophilized and resuspended siRNAs are stored at or PTPRC (CD45), Active P50-21H below -20oC. After resuspension, siRNA stock solutions ≥2 PTPRC (CD45), Active P50-21BG µM can undergo up to 50 freeze-thaw cycles without PTPRE, Active P52-21G significant degradation. For long-term storage, it is PTPRF (LAR), Active P53-21G recommended that the siRNA is stored at -70oC. For most PTPRS(PTP-sigma), P64-21G favorable performance, avoid repeated handling and Active multiple freeze/thaw cycles. PTPRM (PTP-mu) Protein P58-35G Scientific Background PTPRE or protein tyrosine phosphatase receptor type E is a member of the protein tyrosine phosphatase (PTP) family. This family of proteins are known to be signaling PTPRE siRNA Set I molecules that regulate a variety of cellular processes siRNA duplexes targeted against three exon regions including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRE is highly expressed in Catalog # P52-911B brain and testis (1). The regulatory roles of this PTP in Ras Lot # Z2085-30 related signal transduction pathways, cytokines induced Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol signaling, as well as the activation of voltage-gated K+ Format Lyophilized powder channels have been identified. PTPRE has a Stability 1yr at -70oC from date of shipment transmembrane segment and a cytoplasmic region Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is containing duplicated PTPase domains (2). recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can 1. Yi, T. et.al: Identification of novel protein tyrosine undergo up to 50 freeze-thaw cycles phosphatases of hematopoietic cells by polymerase without significant degradation. For chain reaction amplification. Blood 78: 2222-2228, long-term storage, it is recommended that the siRNA is stored at -70oC. For 1991. most favorable performance, avoid 2. Krueger, N. X. et.al: Structural diversity and evolution repeated handling and multiple of human receptor-like protein tyrosine freeze/thaw cycles. phosphatases. EMBO J. 9: 3241-3252, 1990. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .
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