Propagation of Bulbous Ornamentals by Simple Cultures of Bulb-scale Segments Using Plastic Vessels

Tadashi Yanagawa Laboratory of Horticultural Science Faculty of Education Kyoto University of Education Kyoto Japan

Keywords: ornamental bulbs, bulblet regeneration, , Liliaceae, simple culture, twin-scale segments, single-scale segments

Abstract The purpose of this study was to investigate bulblet regeneration of bulb-scale segments excised from bulbs of 26 and eight garden cultivars in the Amaryllidaceae and Liliaceae using plastic vessels. Moistened horticultural vermiculite or Japanese cedar bark were distributed to two types of plastic vessels, polyethylene bags (250 x 350 mm) and polyvinyl chloride cylinder vessels (130 x 100 mm). Cultures of twin-scale segments with basal plate using the plastic vessels afforded high bulblet regeneration for the majority of species tested. In the species and the garden cultivars belonging to the Liliaceae, not only single-scale segments with basal plate but also those without basal plate induced bulblets at a high rate regardless of the species. In contrast, in most of the species in the Amaryllidaceae, the single-scale segments with basal plate showed a clear tendency to produce bulblets at a higher rate than the ones without basal plate. In these cultures, a high regeneration rate was found for segments with cutting treatments to a third of the segment length from the proximal ends of the segments. The simple cultures of the bulb-scales segments using the plastic vessels proved to be feasible for artificial induction of bulblets in most of the species including lilies.

INTRODUCTION Many bulbous species produce few natural offsets, and some are not readily grown from seed. Scaly lily bulbs are multipled by scaling, where detached bulb-scales are planted in moist vermiculite, sand and so forth, and produce bulblets. For clonal propagation of Hippeastrum bulbs with a tunicate bulb, the fractional scale-stem cutting (stem cuttage) method has been widely used since Traub’s (1935) reports. The cuttings were planted in compost, such as vermiculite, peatmoss, and developed bulblets. Sakanishi and Yanagawa (1979) applied this scale-stem cutting method to the tunicate bulbs of 21 species in the Amaryllidaceae and Liliaceae. For clonal propagation of Narcissus bulbs, the twin-scaling method was developed to overcome their low natural rates of bulb production (Alkema, 1975; Hanks and Rees, 1979; Hanks, 1985, 1987; Hanks et al., 1986). In twin-scaling, the parent bulb is divided into a number of equal parts, which are further divided into twin-scale segments containing two-scales and basal plate. The twin-scale segments produce adventitious bulblets after incubation in moist vermiculite in polyethylene bags in about 3 months. The twin-scaling method using polyethylene bags is superior in easy incubation to the cutting method. Yanagawa (1988, 1993) applied this twin-scaling method to the tunicated bulbs of 20 species in the Amaryllidaceae and Liliaceae. This paper describes the regenerative response of various bulb-scale segments excised from bulbs of 26 species and eight garden cultivars in the Amaryllidaceae and the Liliaceae by simple culture using polyethylene bags and polyvinyl chloride vessels.

Proc. IXth Intl. Symp. on Flower Bulbs 343 Eds.: H. Okubo, W.B. Miller and G.A. Chastagner Acta Hort. 673, ISHS 2005

MATERIALS AND METHODS Bulbs were taken from of 26 species and eight garden cultivars in the Amaryllidaceae and Liliaceae, grown in the field and greenhouses at Kyoto University of Education. The bulbs were treated in aqueous benomyl fungicide (0.2%) for 20 minutes. In tunicate bulbs, the sterilized bulbs were divided into about eight equal longitudinal parts. The number of parts cut from each bulb depended on the size of the bulb. Each part was further divided into segments containing a single-scale and twin-scales by cutting through basal plate, starting from the outside. Each part thereby yielded two to six segments with basal plate, the thick, angular innermost scales being discarded. In these cases, single-scale segments without basal plate were also prepared. In scaly bulbs, lily bulb-scale segments were detached from each sterilized bulb of three species and eight garden cultivars. The outer brown or injured scales and the inner small scales were removed. In some experiments, cuts were made from the basal (proximal) end of the twin-scale segments or lily bulb-scale segments. These cuts were one third of the length of the segment. The single-scale segments, the twin-scale segments and the lily bulb-scale segments were incubated in polyethylene bags (25 x 35 cm, 0.22 mm thick) or polyvinyl chloride vessels (13 cm diameter cylinder with lid, 10.5 cm height) containing horticultural vermiculite, Japanese cedar bark, peatmoss, perlite or sawdust, generally with 15 to 50 segments, 800 ml or 350 ml dry vermiculite and 80 ml ion-exchanged water. The vessels were sealed with rubber bands or tape so that there was an air space above the vermiculite, and stored for 12 weeks in the dark at 25°C. Three plastic vessels were used for each treatment. At the end of this incubation period, the number of bulblets produced on each segment was recorded.

RESULTS We examined ehe effect of the quantity of water in the vermiculite on bulblet regeneration of Hippeastrum twin-scale segments and Lilium bulb-scale segments in polyethylene bags. With Hippeastrum twin-scale segments, higher bulblet regeneration was shown in the medium containing 80 ml of water. In the cases of Lilium segments, higher bulblet regeneration was found in each quantity (40, 80, 160 ml) of water (data not shown). Hippeastrum twin-scale segments and Lilium bulb-scale segments were cultured with various media including vermiculite, peatmoss, perlite, sawdust or Japanese ceder bark using polyethylene bags and polyvinyl chloride vessels. In the cultures of Hippeastrum twin-scale segments, higher bulblet regeneration was shown in vermiculite and Japanese ceder bark media. The other media containing peatmoss, perlite or sawdust showed lower regenerative ability. In the cases of Lilium bulb-scale segments, higher bulblet regeneration was shown in Japanese cedar bark, vermiculite and peatmoss media. By polyvinyl chloride vessel culture instead of polyethylene bag culture, Hippeastrum twin-scale segments and Lilium bulb-scale segments also produced bulblets at a high rate regardless of medium (Table 1). In the species belonging to the Liliaceae, not only the single-scale segments with basal plate but also the ones without basal plate induced bulblets at a high rate regardless of the species. In contrast, bulblet regeneration of the species in the Amaryllidaceae differed according to the species. Namely, in most of the species, the single-scale segments with basal plate showed a clear tendency to produce bulblets at a higher rate than the ones without basal plate. With Cyrtanthus, Leucojum and x Amarcrinum, however, no difference in the regenerative rate was shown between those segments. The segments excised from Nerine bulbs showed no bulblet regeneration (Fig. 1).

344 When the bulb-scale segments detached from various lily bulbs, Lilium longiflornm, L. speciosum, L. leichtlinii var. maxcimowiczi, six Asiatic hybrid cultivars, one Aurelian hybrid cultivar and one Oriental hybrid cultivar, were cultured in polyethylene bags, the bulb-scale segments of every species and cultivar tested, produced bulblets at the rate of over 71%. To increase regenerative ability of Hippeastrum twin-scale segments, the segments with cuts from their proximal ends were cultured. As the number of cuts increased, regenerated bulblets increased. In Hippeastrum, the 2- and 3-cut treatments gave the highest number of bulblets. For the bulb-scale segments of Lilium longiflorum, the number of regenerated bulblets increased with the number of cuts and the 4-cut treatment gave the highest number of bulblets (Table 2).

DISCUSSION In this propagation, the size of the segments is important for producing bulblets, namely, the number of bulb-scales in the segments. In a comparison of the number of scales in the segments with respect to bulblet regeneration, three-scale segments showed a clear tendency to produce bulblets at a higher rate than both the twin-scale and the single-scale segments (Yanagawa, 1988). The present results showed that cultures of twin-scale segments afforded high bulblet regeneration for the majority of species tested and single-scale segments excised from most species also produced bulblets. The number of single-scale segments per bulb was greater than the other segments. As a result, the single-scale segments of some species obtained the highest number of bulblets per bulb. Bulblet regeneration was stimulated by basal cutting, with the maximum increase seen with 3- or 4-cuts per segment. Yanagawa and Osaki (1996) also found that cut treatments to bulb-scale base explants improved in vitro propagation of Hippeastrum. For the clonal propagation of Narcissus, instead of twin-scaling, the simpler, less productive, but apparently more reliable method of chipping has been promoted (Flint, 1982; Vreeburg, 1986; Linfield and Price, 1990; Fenlon et al., 1990). In chipping, 8-16 chips containing a number of scales without further division into twin-scales are excised from each bulb and incubated normally. These segments produced adventitious bulblets from the basal scale portion close to the junction with basal plate (Grootaarts et al., 1981). Bulblet regeneration, especially of the Amaryllidaceae, is confined to the segments from the portion near scale base (Yanagawa and Sakanishi, 1977, 1980). In contrast, in the species in the Liliaceae, not only the scale base but also the segments from the middle and the apical portions of scale induced bulblets (Robb, 1957; Hackett, 1969; Pierik and Ruibing, 1973; Yanagawa, 1989). Particularly, Hyacinthus bulbs were propagated successfully by leaf cutting (Krause, 1977). The present results also showed a higher regenerative capacity for the segments of the species in the Liliaceae than for those in the Amaryllidaceae. These simple cultures using polyethylene bags and polyvinyl chloride vessels for the bulb-scale segments proved to be feasible for artificial induction of bulblets in most of the species tested, especially for lily bulb propagation. The cut treatments to the segments were effective for increasing regenerative ability.

Literature Cited Alkema, H.Y. 1975. Vegetative propagation of daffodils by double-scaling. Acta Hort. 47:193-199. Fenlon, J.S., Jonnes, S.K., Hanks, G.R. and Langton, F.A. 1990. Bulb yields from narcissus chipping and twin-scaling. J. Hort. Sci. 65:441-450. Flint, G.J. 1982. Kirton’s up on narcissus chips. Grower 98(22):24, 29. Grootaarts, H., Schel, J.H.N. and Pierik, R.L.M. 1981. The origin of bulblets formed on excised twin scales of Nerine bowdenii W. Watts. Cell Tissue and Organ

345 Culture 1:39-46. Hackett, W.P. 1969. Aseptic multiplication of lily bulblets from bulb scales. Proc. Intern. Plant Prop. Soc. 19:105-108. Hanks, G.R. and Rees, A.R. 1979. Twin-scale propagation of narcissus: a review. Scientia Hort. 10:1-14. Hanks, G.R. 1985. Factors affecting yields of adventitious bulbils during propagation of Narcissus by the twin-scaling technique. J. Hort. Sci. 60:531-543. Hanks, G.R., Shalk, G. and Jones, S.K. 1986. Bulbil production in Narcissus: the effect of temperature and duration of storage on bulb unit development and subsequent propagation by twin-scaling. Ann. Appl. Biol. 109:417-425. Hanks, G.R. 1987. Effects of growth retardants on bulbil production by Narcissus twin-scales. Ann. Appl. Biol. 110:203-297. Krause, J. 1977. Vermehrung von Hyazinthen durch Blattstecklinge. Gartenbau. 24:279-281. Linfield, C.A. and Price, D. 1990. Effect of fungicides on the production of adventitious bulbils in the propagation of Narcissus by the chipping technique. Crop Protection 9:143-147. Pierik, R.L.M. and Ruibing, M.A. 1973. Regeneration of bulblets on bulb scale segments of hyacinth in vitro. Neth. J. Agric. Sci. 21:129-138. Robb, S.M. 1957. The culture of excised tissue from bulb scales of Lilium speciosum Thunb. J. Exp. Bot. 8:348-352. Sakanishi, Y. and Yanagawa, T. 1979. Bulblet formation by fractional scale-stem cuttings in various bulbous ornamentals (in Japanese). Studies from the Institute of Horticulture, Kyoto University 9:100-107. Traub, H.P. 1935. Propagation of amaryllis by stem cuttage. Amer. Amaryl. Soc. Yrbk. p.123-126. Vreeburg, R.J.M. 1986. Chipping of narcissus bulbs: A quick way to obtain large numbers of small, round bulbs. Acta Hort. 177:579-584. Yanagawa, T. and Sakanishi, Y. 1977. Regeneration of bulblets on Hippeastrum bulb segments excised from various parts of a parent bulb (in Japanese). J. Japan. Soc. Hort. Sci. 46:250-260. Yanagawa, T. and Sakanishi, Y. 1980. Studies on the regeneration of excised bulb tissues of various tunicated-bulbous ornamentals. I. Regenerative capacity of the segments from different parts of bulb scales (in Japanese). J. Japan. Soc. Hort. Sci. 48:495-502. Yanagawa, T. 1988. Propagation of tunicated-bulbous ornamentals by twin-scaling (in Japanese). Abstracts Fall Meeting, Japan. Soc. Hort. Sci. p.566-567. Yanagawa, T. 1989. Studies of the scale propagation of lilies (in Japanese). J. Japan. Soc. Hort. Sci. 58(Suppl. 2):468-469. Yanagawa, T. 1993. Propagation of bulbous ornamentals by polyethylene bag culture of single-scale segments. J. Regional Education Center (REC), Agricultural Technology 2:83-88. Yanagawa, T. and Osaki, T. 1996. In vitro propagation of bulblets and elimination of viruses by bulb-scale cultures of Hippeastrum hybridum bulbs. Plant Tissue Culture Letters 13(2):147-152.

346 Tables

Table 1. Bulblet regeneration of Hippeastrum twin-scale segments and Lilium longiflorum bulb-scale segments cultured on various medium using plastic vessels.

Plastic Medium Hippeastrum Lilium longiflorum vessel twin-scale segment bulb-scale segment Percentage Number of Percentage Number of of bulblet bulblets per of bulblet bulblets per regeneration segment regeneration segment Polyethylene Vermiculite 97±3.5 az 1.5±0.06 94±2.3 a 1.2±0.06 bag Peatmoss 83±3.5 a 1.3±0.06 92±1.7 a 1.3±0.06 Perlite 62±1.7 b 1.3±0.06 84±2.3 a 1.1±0.06 Sawdust 66±2.3 b 1.3±0.06 84±2.3 a 1.1±0.06 Japanese 99±1.7 a 1.9±0.10 99±1.7 a 1.8±0.10 cedar bark Polyvinyl Vermiculite 94±2.3 a 1.3±0.06 99±1.7 a 1.7±0.10 chloride Japanese 99±1.7 a 1.5±0.06 99±1.7 a 1.9±0.10 vessel cedar bark 20 to 30 segments were cultured in the polyethylene bag (25 x 35 cm) containing 800 ml dry medium and 80 ml ion-exchanged water or in the polyvinyl chloride vessel (13 cm diameter, 10.5 cm height) containing 350 ml medium and 80 ml ion-exchanged water for 12 weeks. Three plastic vessels were used for each treatment. Values are mean ± SE of 3 vessels. z Different letters within column indicate significant difference by Tukey’s test at 5% level.

Table 2. Effects of cut treatments on the proximal ends of Hippeastrum twin-scale segments with basal plate and Lilium bulb-scale segments on bulblet regeneration.

Species, segment Number Percentage of bulblet Number of bulblets of cuts regeneration per segment Hippeastrum hybridum 0 98±2.9 az 1.3±0.06 twin-scale segment 1 98±2.9 a 1.8±0.10 2 91±3.2 a 2.5±0.06 3 88±2.6 a 2.5±0.15 Lilium longiflorum 0 93±2.5 a 1.1±0.06 bulb-scale segment 1 98±2.6 a 1.7±0.06 2 98±2.9 a 2.1±0.15 3 97±2.9 a 2.5±0.06 4 98±2.9 a 2.7±0.06 15 to 20 segments with cuts in their proximal ends were cultured in the polyethylene bag containing 800 ml vermiculite and 80 ml ion-exchanged water for 12 weeks. Three polyethylene bags were used for each treatment. Values are mean ± SE of 3 bags. z Different letters within column indicate significant difference by Tukey’s test at 5% level.

347 Figures

Single-scale segment Single-scale segment Twin-scale segment with basal plate with basal plate without basal plate

Amarylliadaceae Amaryllis belladonna Crinum powellii Cyrtanthus mackenii Haemanthus albiflos Hippeastrum hybridum speciosa Leucojum aestivum Lycoris sprengeri Nerine sarniensis Rhodophiala bifida Sternbergia lutea Zephyranthes candida ×Amarcrinum ×Amarine ×Hippeaskelia

Liliace Albuca nelsonii Eucomis undulata Galtonia candicans Hyacinthus orientalis Ornithogalum arabicum Ornithogalum longibractaetum Scilla hyacinthoides Scilla peruviana

0 20 40 60 80 100 0204060801000 20406080100 Percentage of bulbet regeneration (%)

Fig.1Fig. 1.Bulblet Bulblet regeneration regeneration by by polyethylene polyethylene bag bag culture culture of of variousvarious bulb-scale segmentssegments excisedexcised from bulbs from in bulbs the Amaryllidaceae in the Amaryllidaceae and the Liliaceae.and Liliaceae. 15 to 50 segments were 15 to 50cultured segments in were the culturedpolyethylene in the polyethylenebag containing bag 800containing ml vermiculite 800ml vermiculite and 80 andml 80 ml ion-exchangedion-exchanged waterwater forfor 12 12 weeks. weeks. Three Three polyethylenepolyethylene bagsbags werewere usedused for each each treatment.treatment. Values Valuesare mean are ± mean SE of ± 3SE bags. of three bags.

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