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Tecan Journal Life Sciences and Partnering Business Edition 3/2013 Tecan Journal Life Sciences and Partnering Business Edition 3/2013 A turnkey solution for forensic identification Uruguay, Latin America pages 24-25 Enhancing clinical diagnostics in Turkey pages 12-13 Meeting the demands of mass spectrometry sample preparation pages 16-17 Boosting capacity for veterinary testing in the USA pages 26-27 2 CEO WELCOME TECAN JOURNAL 3/2013 Welcome to the latest Tecan Journal This June was an exciting time for Tecan dedicated system development capabilities, with the successful completion of the OEM complemented by the Tecan Cavro® brand development of the Dako Omnis, the latest components range. A Freedom EVO® platform major project for our Partnering Business. for mass spectrometry sample preparation The Dako Omnis, a fully automated tissue- completed the picture, ensuring that customers based cancer diagnostics platform, was headed home from Houston knowing that co-developed with our OEM partner Dako, they could have confidence in Tecan’s 30 years an Agilent Technologies Company, and is of expertise, global coverage and proven manufactured by Tecan. It sets new standards reputation for quality and innovation. in advanced tissue staining, automating both immunohistochemistry (IHC) and in situ In this issue, we learn more about the hybridization (ISH) processes, offering hospitals benefits of automated mass spectrometry and clinical laboratories greater flexibility, sample preparation, discover how the efficiency and traceability than ever before. HP D300 Digital Dispenser is enhancing drug discovery experiments, and hear from Another important event this summer was students of the Tecan Academy. Finally, no the annual AACC Clinical Lab Expo, held from edition would be complete without the the 30th of July to the 1st of August in Houston, usual array of stories describing some of the Texas. Tecan’s booth generated enormous innovative and interesting applications that interest, with visitors seeing at first hand the our customers around the world have for many reasons why the Company is the partner their Tecan equipment. of choice in key areas of clinical diagnostics. Highlights included the state-of-the-art Dako I hope you enjoy the journal, Omnis system and a focus on Tecan’s OEM David Martyr, CEO New offices for Tecan France and Tecan Ibérica: Tecan France S.A.S.U. Tecan Ibérica Instrumentación S.L. 6, Avenue du Château de Gerland Torre Mapfre F-69 007 LYON C/ de la Marina 16-18, Planta 11a C-1 T +33 4 72 76 04 80 E-08005 Barcelona F +33 4 72 76 04 99 T +34 935 95 2531 [email protected] F +34 93 3308700 [email protected] Welcome CONTENTS TECAN JOURNAL 3/2013 3 page 7 Shanghai’s Changhai Hospital has successfully evaluated Tecan’s HS 400™ Pro hybridization station 2 CEO Welcome 4 Automation doesn't get easier than this! pages 8-9 4 Tecan and Promega offer real-time cytotoxicity analysis 5 Automated mass spectrometry sample preparation; the key to an efficient workflow 5 Go configure! Receive a customized quote fast! 6 Tecan Academy offers web-based training for new users 7 Automation helps to improve in situ hybridization protocols 8 - 9 Shining a light on novel cancer drugs 10 - 11 Pushing back boundaries with ultra- low volume pipetting 12 - 13 Enhancing clinical diagnostics 14 - 15 Boosting drug discovery with pages 14-15 efficient transfection technology 16 - 17 Meeting the high throughput demands of mass spectrometry sample preparation 18 - 19 Removing the constraints of manual dose response assays 20 - 21 Novartis embraces full automation 22 - 23 New possibilities for biological pages 22-23 surfaces research 24 - 25 A turnkey solution for forensic Researchers at McMaster identification University’s Biointerfaces 26 - 27 Boosting capacity for veterinary Institute rely on the testing flexibility and modularity 27 Leading the debate of their Tecan workstations 28 Events Contents 4 PRODuct NEWS TECAN JOURNAL 3/2013 Automation doesn’t get easier than this! Tecan’s Smart Automation solutions offer straightforward liquid handling for new users Tecan is launching a range of benchtop The first Smart Automation solutions to A dedicated user interface and easy-to-follow solutions designed to provide fast, simple be launched are: application guide ensure that even and cost-effective automation of liquid Pickolo™ colony picking; for fully inexperienced users can get their systems up handling tasks. Based on the Freedom EVO® 75 and running quickly, with touchscreen operation automated colony picking platform, these preconfigured systems are also available for some applications. These designed to eliminate tedious manual liquid NucleoSpin® purification; for fully complete solutions provide an ideal introduction handling tasks without the need for previous automated, vacuum-based nucleic acid to the throughput, precision and reproducibility automation experience. extraction using MACHEREY-NAGEL offered by Tecan’s liquid handling systems, NucleoSpin kits with the flexibility to expand and adapt as your Each Smart Automation solution is supplied NucleoMag® purification; for fully laboratory needs change. with optimized, ready-to-run protocols, and automated, magnetic bead-based includes all of the modules and ancillary nucleic acid separation using To find out more on Tecan’s devices necessary to automate your MACHEREY-NAGEL NucleoMag kits. Smart Automation solutions, visit manual processes. www.tecan.com/smartautomation Tecan and Promega offer Application Note real-time cytotoxicity analysis oriented functions of the Infinite 200 PRO – including the Gas Control Module (GCM™), Optimal Read (OR), linear and orbital shaking, temperature control and automated z-focusing – offers an unparalleled solution Viable cell Non-viable for long-term kinetic studies of cytotoxicity. cell By providing stable culture conditions within the reader chamber, this set-up allows Low fluorescence High fluorescence continuous measurement over the duration The CellTox Green Dye is excluded from viable cells, but of the experiment, ensuring high quality data binds to DNA from cells with compromised membrane integrity, resulting in an increase in fluorescence Tecan has worked with Promega to without the need for manual intervention or demonstrate the suitability of the transfer of microplates between an incubator Infinite® 200 PRO and HP D300 Digital and the reader. prevent a bolus of DMSO from sinking onto Dispenser for real-time kinetic investigation plated cells, automatic plate randomization of cytotoxicity using Promega’s CellTox™ The assay workflow can be further improved and straightforward generation of drug-drug Green Dye. by use of the HP D300 Digital Dispenser interaction studies – the HP D300 is designed to provide on -demand assay plate set-up. to increase productivity with minimal set -up The CellTox Green Cytotoxicity Assay measures Offering non-contact dispensing from 13 pl to time for cell-based and biochemical studies. changes in membrane integrity that occur as 10 μl, this innovative instrument allows small a result of cell death. This non-toxic dye allows molecules in DMSO to be dispensed directly To find out more on Tecan’s Infinite 200 PRO real-time fluorescence-based measurements into the assay plate, eliminating the need for and GCM, visit www.tecan.com/gcm for up to 72 hours, and is ideally suited to time -consuming and wasteful serial dilutions. mode-of-action studies. Combining the With a range of features specifically tailored To learn more about Direct Digital CellTox Green assay with the advanced towards cell-based and drug discovery Dispensing, go to environmental control and cell biology applications – including plate shaking to www.tecan.com/digitaltitration Real-Time Cytotoxicity Analysis 1 1 2 2 Tracy Worzella , Andrew Niles , Thomas Hengstl , Michael Fejtl , 2 3 Christian Oberdanner and Jessica Merlino 1 Promega Corporation, Madison, WI · 2 Tecan Austria GmbH · 3 Tecan Group, Ltd., Männedorf, Switzerland Abstract We demonstrate the use of a non-activity-based cytotoxicity Using DNA binding as an indicator of cytotoxicity mitigates this probe, CellTox™ Green Dye, which can be added at the issue, providing a robust method of detection for time course beginning of an experiment and employed in real time experiments. CellTox™ Green Dye is an asymmetric cyanine for the kinetic determination of cytotoxicity. We used the dye that measures cytotoxicity as a result of compromised HP D300 Digital Dispenser, available from Tecan, for cellular membrane integrity (Figure 1). noncontact dispensing of test compound and the Tecan When the dye binds to DNA, green fluorescence is enhanced. Infinite® M200 PRO with Gas Control Module (GCM™) The signal intensity is directly correlated to the degree of for kinetic measurement of cytotoxicity in HepG2 cells change in the membrane integrity. The dye can be added at over 72 hours. the time of cell plating or compound treatment and the fluorescence monitored kinetically over a period of up to 72 Introduction hours. Alternatively, the dye can be added at the conclusion of compound treatment and an endpoint measurement taken. In vitro cytotoxicity is largely influenced by test article concentration and the exposure period with cells. The diversity of kinetic response can complicate conventional cytotoxicity endpoint assay determinations because most assay reagents are formulated to measure enzymatic biomarkers that are susceptible to time-dependent decay. 1 PRODuct News TECAN JOURNAL 3/2013 5 Automated mass spectrometry sample preparation; the key to an efficient
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