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Downloaded from http://www.jimmunol.org/ by guest on September 25, 2021 is online at: average * The Journal of Immunology , 19 of which you can access for free at: 2011; 186:164-173; Prepublished online 24 from submission to initial decision 4 weeks from acceptance to publication November 2010; doi: 10.4049/jimmunol.0904170 http://www.jimmunol.org/content/186/1/164 H5N1 Virus Activates Signaling Pathways in Human Endothelial Cells Resulting in a Specific Imbalanced Inflammatory Response Dorothee Viemann, Mirco Schmolke, Aloys Lueken, Yvonne Boergeling, Judith Friesenhagen, Helmut Wittkowski, Stephan Ludwig and Johannes Roth J Immunol cites 48 articles Submit online. Every submission reviewed by practicing scientists ? is published twice each month by Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts http://jimmunol.org/subscription http://www.jimmunol.org/content/suppl/2010/11/24/jimmunol.090417 0.DC1 This article http://www.jimmunol.org/content/186/1/164.full#ref-list-1 Information about subscribing to The JI No Triage! Fast Publication! Rapid Reviews! 30 days* Why • • • Material References Permissions Email Alerts Subscription Supplementary The Journal of Immunology The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. This information is current as of September 25, 2021. The Journal of Immunology H5N1 Virus Activates Signaling Pathways in Human Endothelial Cells Resulting in a Specific Imbalanced Inflammatory Response Dorothee Viemann,*,†,‡ Mirco Schmolke,x Aloys Lueken,*,‡ Yvonne Boergeling,x Judith Friesenhagen,* Helmut Wittkowski,*,† Stephan Ludwig,‡,x and Johannes Roth*,‡ H5N1 influenza virus infections in humans cause a characteristic systemic inflammatory response syndrome; however, the molec- ular mechanisms are largely unknown. Endothelial cells (ECs) play a pivotal role in hyperdynamic septic diseases. To unravel specific signaling networks activated by H5N1 we used a genome-wide comparative systems biology approach analyzing gene ex- pression in human ECs infected with three different human and avian influenza strains of high and low pathogenicity. Blocking of specific signaling pathways revealed that H5N1 induces an exceptionally NF-kB–dependent gene response in human endothelia. Downloaded from Additionally, the IFN-driven antiviral program in ECs is shown to be dependent on IFN regulatory factor 3 but significantly impaired upon H5N1 infection compared with low pathogenic influenza virus. As additional modulators of this H5N1-specific imbalanced gene response pattern, we identified HMGA1 as a novel transcription factor specifically responsible for the over- whelming proinflammatory but not antiviral response, whereas NFATC4 was found to regulate transcription of specifically H5N1- induced genes. We describe for the first time, to our knowledge, defined signaling patterns specifically activated by H5N1, which, in contrast to low pathogenic influenza viruses, are responsible for an imbalance of an overwhelming proinflammatory and http://www.jimmunol.org/ impaired antiviral gene program. The Journal of Immunology, 2011, 186: 164–173. nfluenza A viruses still pose a major threat due to their cell (EC) tropism (9–13). Recently, a-2,3-linked sialic acid re- pandemic potential. There is a huge natural virus reservoir in ceptors preferentially bound by avian influenza virus were shown to I birds, which provides a pool of viral genes that contribute to be present on human ECs, suggesting a crucial involvement of ECs the generation of novel pandemic virus strains. The highly path- in the characteristics of systemic H5N1 diseases (14). Isolated ogenic avian H5N1 virus is the first example of an avian virus re- suppression or knockdown of the cytokine response did not pro- ported to have infected and killed several hundreds of humans (1). tect against lethal outcomes (13, 15, 16). In human bronchial epi- by guest on September 25, 2021 Human-adapted influenza A viruses cause primarily infections of thelial cells, high pathogenic H5N1 variants replicate stronger than the upper respiratory tract with limited virus replication. Highly low pathogenic variants or the human H3N2 virus but elicit a much pathogenic avian influenza viruses (HPAIVs) rather cause systemic weaker and delayed IFN response (17). The overall pattern of infections with hemorrhagic sepsis in poultry. In humans, clinical proinflammatory and antiviral signaling pathways activated by manifestations of H5N1 infections are a systemic inflammatory H5N1 in specific tissues might be the clue to the pathogenicity of response syndrome (SIRS) leading to multiorgan failure (2–6). HPAIVs. There are two characteristics of H5N1 infections: an unusual The cardinal problem of treating H5N1 infections with neur- strong cytokine response (7, 8) and a neuro- as well as endothelial aminidase inhibitors is that it has to be initiated very early, oth- erwise virus replication and dissemination has already progressed too far (6). Evaluating the role of endothelial response programs x *Institute of Immunology, ‡Interdisciplinary Center of Clinical Research, and Institute with respect to proinflammatory and antiviral strategies and de- of Molecular Virology, University of Muenster; and †Department of Pediatrics, Uni- versity Hospital of Muenster, Muenster, Germany lineating the responsible signaling cascades will allow the de- velopment of novel therapeutic strategies. Received for publication December 28, 2009. Accepted for publication October 29, 2010. To approach to these objectives, we performed comparative This work was supported by the Bundesministerium fu¨r Bildung, Wissenschaft, For- global gene expression studies of ECs infected with HPAIV of the schung und Technologie Zoonosis Network FluResearchNet (Grant 01KI07130). H7N7 and H5N1 subtype and a low pathogenic human influenza D.V. and J.R. were supported by grants from the Interdisciplinary Center of Clinical virus of the H1N1 subtype to identify H5N1 specific gene response Research University of Muenster. subpatterns. In line with recent findings of our group, we confirm Address correspondence and reprint requests to Dr. Dorothee Viemann, Institute of k Immunology, University of Muenster, Roentgenstr. 21, D-48149 Muenster, Germany. a remarkable NF- B dependency of the H5N1-induced gene ex- E-mail address: [email protected] pression program (18), which we demonstrate to be much weaker The online version of this article contains supplemental material. or not relevant for infections with the other influenza strains. Abbreviations used in this paper: BS, binding site; EC, endothelial cell; FC, fold Concurrently, infections with H5N1 were characterized by a strik- change; FPV, A/FPV/Bratislava/79 (H7N7) fowl plague virus; HPAIV, highly patho- ing attenuation of the IFN-b–dependent antiviral response. Fur- genic avian influenza virus; IKK2kd, kinase-dead IkB kinase 2; IRF, IFN regulatory factor; ISRE, IFN-stimulated response element; MDCK, Madin-Darby canine kidney; thermore, we reveal HMGA1 as a novel transcription factor to be MOI, multiplicity of infection; PCA, principal component analysis; p.i., postinfection; specifically responsible for the overwhelming proinflammatory PR8, A/PR8/34 (H1N1); qRT-PCR, quantitative real-time RT-PCR; siRNA, short in- but not antiviral response in H5N1 infections, whereas NFATC4 terfering RNA; SIRS, systemic inflammatory response syndrome; wt, wild-type. was found to be a transcriptional regulator of specifically H5N1- Copyright Ó 2010 by The American Association of Immunologists, Inc. 0022-1767/10/$16.00 induced genes. We describe for the first time, to our knowledge, www.jimmunol.org/cgi/doi/10.4049/jimmunol.0904170 The Journal of Immunology 165 MDCKII were cultured in MEM (PAA Laboratories, Pasching, Austria) containing 10% v/v FCS and 100 U/ml penicillin/0.1 mg/ml streptomycin (13 penicillin/streptomycin) (Life Technologies, Carlsbad, CA). A/PR8/ 34 (H1N1) (PR8) was cultivated in 11-d-old embryonic chicken as de- scribed previuosly (19). Allantoic liquid containing viral particles was harvested 50 h postinfection (p.i.). HUVECs were obtained from Cambrex (Charles City, IA) and cultured as described elsewhere (20, 21). HUVECs between passages 5 and 7 were infected with a multiplicity of infection (MOI) of 5 of each viral strain. For the plaque assay, MDCK cells grown 100% confluent in six-well dishes were washed with PBS and infected with serial dilutions of culture FIGURE 1. Infectibility of HUVECs with different strains of influenza supernatants in PBS containing 0.2% bovine serum albumin, 1 mM MgCl2, viruses. Lysates of uninfected HUVECs (ctrl) and PR8-, FPV-, and H5N1- 0.9 mM CaCl2, 100 U/ml penicillin, and 0.1 mg/ml streptomycin for 30 infected HUVECs were immunoblotted 3, 5, and 8 h p.i. for viral proteins min at 37˚C. The inoculum was aspirated, and cells were incubated with PB1 (86 kDa), M1 (27 kDa), and NS1 (26 kDa). Immunostaining of ERK1/ 2 ml MEM medium containing 0.2% BSA and antibiotics supplemented with 0.6% agar (Oxoid, Cambridge, U.K.), 0.3% DEAE-dextran (GE 2 served as protein loading control. Healthcare Bio-Sciences AB, Uppsala, Sweden), and 1.5% NaHCO3 at 37˚C, 5% CO2 for 2 to 3 d. Virus plaques were visualized by staining with defined signaling patterns specifically activated by H5N1, which, neutral-red or Coomassie blue (0.1% Coomassie
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  • The Methamphetamine-Induced RNA Targetome of Hnrnp H in Hnrnph1 Mutants Showing Reduced Dopamine Release and Behavior

    The Methamphetamine-Induced RNA Targetome of Hnrnp H in Hnrnph1 Mutants Showing Reduced Dopamine Release and Behavior

    bioRxiv preprint doi: https://doi.org/10.1101/2021.07.06.451358; this version posted July 7, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. The methamphetamine-induced RNA targetome of hnRNP H in Hnrnph1 mutants showing reduced dopamine release and behavior Qiu T. Ruan1,2,3, Michael A. Rieger4, William B. Lynch2,5, Jiayi W. Cox6, Jacob A. Beierle1,2,3, Emily J. Yao2, Amarpreet Kandola2, Melanie M. Chen2, Julia C. Kelliher2, Richard K. Babbs2, Peter E. A. Ash7, Benjamin Wolozin7, Karen K. Szumlinski8, W. Evan Johnson9, Joseph D. Dougherty4, and Camron D. Bryant1,2,3* 1. Biomolecular Pharmacology Training Program, Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine 2. Laboratory of Addiction Genetics, Department of Pharmacology and Experimental Therapeutics and Psychiatry, Boston University School of Medicine 3. Transformative Training Program in Addiction Science, Boston University School of Medicine 4. Department of Genetics, Department of Psychiatry, Washington University School of Medicine 5. Graduate Program for Neuroscience, Boston University 6. Programs in Biomedical Sciences, Boston University School of Medicine 7. Department of Pharmacology and Experimental Therapeutics and Neurology, Boston University School of Medicine 8. Department of Psychological and Brain Sciences, University of California, Santa Barbara 9. Department of Medicine, Computational Biomedicine, Boston University School of Medicine *Corresponding Author Camron D. Bryant, Ph.D. Department of Pharmacology and Experimental Therapeutics and Department of Psychiatry 72 E.