perature (27°C). germination was examined over a two-week period two-week a over examined was germination Seed (27°C). perature

pared with the non-treated control. control. non-treated the with pared

third treatment, a pair of pruning shears was used to clip an edge of the seed coat. coat. seed the of edge an clip to used was shears pruning of pair a treatment, third

trated sulfuric acid for 4 min and then rinsing in sterile water three times. times. three water sterile in rinsing then and min 4 for acid sulfuric trated

with a radicle of 3 mm in length was considered successfully germinated. germinated. successfully considered was length in mm 3 of radicle a with

placed in sterile cold water. Acid scarification consisted of placing in concen­ in seeds placing of consisted scarification water. cold Acid sterile in placed rinsed three times with sterile water. Seeds were then divided into four groups with with groups four into divided then were Seeds water. sterile with times three rinsed

were surface sterilized with 1 1 with sterilized surface were

o miiin n eepae o os itrppr npti ihsa om tem­ room at dishes petri in paper filter moist a on placed were and imbibition for

number of available seeds restricted the sample size of the experiment. Forty seeds seeds Forty experiment. of the size sample the restricted seeds available of number application of treatments. treatments. of application

e o ahtetet Se ramns nldd o ae ramn, cd scarifi­ acid treatment, water hot included treatments Seed treatment. each for ten

water for 24 hours, hot water treatment, acid treatment, and nicking. However, However, nicking. and treatment, acid treatment, water hot hours, 24 for water

decline was mainly due to browsing from introduced animals and infestation infestation and animals introduced from browsing to due mainly was decline plant

ture tree on (Wiles et al. 1995, 1996). Wiles et al. (1996) pointed out that the the that out pointed (1996) al. et 1996). Wiles 1995, al. et (Wiles Guam on tree ture

ain ramn wt cnetae slui ai,ad lpig wih ee com­ were which clipping, and acid, sulfuric concentrated with treatment cation

the study was to find treatments to improve the seed germination of of germination seed the improve to treatments find to was study the

there have been no reports on seed pre-treatment of of pre-treatment seed on reports no been have there the nursery from field-collected seeds has been attempted at the University of of University the at attempted been has seeds field-collected from nursery the

tive limestone forest in the Mariana Islands. It is recognized as an endangered endangered an as recognized is It Islands. Mariana the in forest limestone tive

leguminous (MacDicken 1994). Common methods include soaking seeds in in seeds soaking include methods Common 1994). (MacDicken plants leguminous

Seeds of all treatments including the control were then soaked in water overnight overnight water in soaked then were control the including treatments all of Seeds

vice 1993) with a current population of 121 on the island of Rota and only one ma­ one only and of Rota island 121 the on of population current a 1993) with vice

of arthropods. In an effort to increase numbers of of numbers increase to effort an In arthropods. of

Micronesica Micronesica

Guam and the Guam Department of of Agriculture. Department Guam the and Guam

pce yteUie Sae ihadWllf Srie US ihadWllf Ser­ Wildlife and (U.S. Fish Service Wildlife and Fish States United the by species

All seeds which were clipped germinated within one week from the date of of date the from week one within germinated clipped were which seeds All

For the hot water treatment, seeds were placed in boiling water for 3 min, then then min, 3 for water boiling in placed were seeds treatment, water hot the For

S. nelsonii nelsonii S.

Serianthes nelsonii nelsonii

Several pre-treatments were recommended for seed germination of various various of germination seed for recommended were pre-treatments Several

30(2): 439-440 30(2):

Effect of Pre-treatments on Seed Germination Germination Seed on Pre-treatments of Effect

seeds were collected from Guam's single mature tree. The limited limited The tree. mature single Guam's from collected were seeds

College of Agriculture and Life Sciences, University of Guam, Guam, of University Sciences, Life and of Agriculture College

of of

JEANNY RICHARDSON AND AND RICHARDSON JEANNY

Serianthes nelsonii nelsonii Serianthes

, ,

1997 1997

303 University Drive, Mangilao GU 96923 96923 GU Mangilao Drive, University 303

erl i nedmc euios re ht rw i na­ in grows that tree leguminous endemic an is Merrill

In In

% %

contrast, no seed germination was observed in the the in observed was germination seed no contrast,

sodium hypochlorite (NaOCl) for 15 min and were were 15 and min for (NaOCl) hypochlorite sodium

Note Note

Merrill () (Fabaceae) Merrill

MARI MARI

S. nelsonii nelsonii S.

MARUTANI MARUTANI

S. nelsonii. nelsonii. S.

plant propagation in in propagation plant

The objective of of objective The

S. nelsonii. nelsonii. S.

. .

A seed seed A

In In the the

Serianthes Serianthes nel­

(Fabaceae), (Fabaceae), an en­

C. C. Manglona. 1996.

J. J.

& &

At At the end of the experiment, all

Grout. Grout. 1995. The status and distri­

J. J.

Serianthes Serianthes nelsonii

References References

S. S. nelsonii.

Davis Davis & D.

Micronesica Micronesica 30(2), 1997

W W

Aguon, Aguon, G.

. .

F. F.

. .

I. I. H. Schreiner, Nafus, D. L. K. Jurgensen

C. C.

U.S. U.S. Fish and Wildlife Service, Portland, OR. 60pp.

J., J.,

J., J.,

dangered dangered Micronesian tree. Biological Conservation 76:229-239.

The The status, biology and conservation of

28:31-49

Winrock Winrock International, Morrilton, Arkansas, Bangkok. and FAO, 272pp.

bution bution of endangered animals and plants in northern Guam. Micronesica

sonii. sonii.

Received 2 May May 1997 2 Received

Wiles, Wiles, G.

Wiles, Wiles, G.

MacDicken, MacDicken, K. G. 1994. Selection and Management of Nitrogen-fixing Trees.

conservation conservation program of this endangered species. United United States Fish & Wildlife Service. 1993. Recovery Plan for

seeds seeds were clipped and seedlings were planted in the nursery as part a of the plant

other other treatments and the control. It was observed that seeds with the sulfuric acid

treament treament swelled, suggesting that a longer treatment with sulfuric acid might in­

440 440 ping ping can improve germination of prove prove seed germination. The present experiment has demonstrated that seed clip­