Supplementary Table 1. List of CMV Epitopes Used for in Vitro Stimulation

Supplementary table 1. List of CMV epitopes used for in vitro stimulation.

HLA Amino acid CMV protein Code Epitope sequence restriction location (gene) A1 VTE VTEHDTLLY 245-253 pp50 (UL44) YSE YSEHPTFTSQY 363-373 pp65 (UL83) A2 NLV NLVPMVATV 495-503 pp65 (UL83) VLE VLEETSVML 316-324 IE-1 (UL123) A3 IMR IMREFNSYK 683-691 gB (UL55) TTV TTVYPPSSTAK 945-955 pp150 (UL32) A11 SVL SVLGPISGHVLK 13-24 pp65 (UL83) A24 FTS FTSQYRIQGKL 369-379 pp65 (UL83) QYD QYDPVAALF 341-349 pp65 (UL83) B7 KAR KARDHLAVL 101-109 pp65 (UL83) QAR QARLTVSGL 158-166 pp65 (UL83) RPH RPHERNGFTVL 265-275 pp65 (UL83) TPR TPRVTGGGAM 417-426 pp65 (UL83) B8 ELR ELRRKMMYM 199-207 IE-1 (UL123) QIK QIKVRVDMV 88-96 IE-1 (UL123) B27 RRR RRRHRQDAL 539-547 pp65 (UL83) B35 CPS CPSQEPMSIYVY 103-114 pp65 (UL83) FPT FPTKDVAL 188-195 pp65 (UL83) IPS IPSINVHHY 123-131 pp65 (UL83) B40/60 CED CEDVPSGKL 232-240 pp65 (UL83) B44 QEF QEFFWDANDIY 511-521 pp65 (UL83) QEP QEPMSIYVY 106-114 pp65 (UL83) Cw4 QAI QAIRETVEL 331-339 pp65 (UL83) QYD QYDPVAALF 341-349 pp65 (UL83) Cw6 TRA TRATKMQVI 211-219 pp65 (UL83) DR7 DYS DYSNTHSTRYV 217-227 gB (UL55) DR11 HEL HELLVLVKKAQL 276-287 gH (UL75)

Supplementary table 2. List of epitopes used for in vitro T cell expansion and patient responses.

GBM:ID peptides used specificity of expanded T cells

01 NLV, VLE, TPR, QEF TPR, VLE

02 NLV, VLE, IMR, TTV, KAR, TPR, QAR TPR

03 VTE, YSE, SVL, QIK, ELR, RRR VTE, YSE, QIK, ELR

04 NLV, VLE, QEF, QEP NLV, VLE, QEF

05 IMR, TTV, QEF, QEP not done (low cell numbers)

06 VTE, YSE, NLV, VLE, QIK, ELR, RRR VTE, YSE, ELR, QIK

07 IMR, TTV, KAR, TPR, QAR, RPH, CED RPH, CED, TPR

09 VTE, YSE, NLV, VLE, QIK, ELR, RRR, QEF, VTE, YSE, QIK, ELR QEP

11 VTE, YSE, FTS, QYD, FPT, CPS, IPS, QAI VTE, YSE, FPT, QAI, CPS, QYD

12 IMR, TTV, FTS, QYD, TRA no expansion

13 VTE, YSE, IMR, TTV, KAR, TPR, QAR, VTE, YSE, TPR, RPH, ELR, QIK RPH, RRR, ELR, QIK

14 VTE, YSE, FTS, QYD, KAR, TPR, QAR, VTE, YSE, QYD, TPR, QAR, RPH RPH

15 NLV, VLE, IMR, TTV, QEF, QEP, CED NLV, VLE

16 VTE, YSE, NLV, VLE, KAR, TPR , QAR, VTE, VLE, TPR, RPH, IPS, HEL RPH, FPT, CPS, IPS, HEL

19 TRA, DYS, QYD QYD

Rows highlighted in grey indicate T cell products that could not be administered to patients, either because of withdrawal from the trial due to progressive disease (GBM:ID 07, 11, 14) or process failure (GBM:ID 05, 12).

Supplementary table 3. List of genes included in taqman array.

Functional group Gene name Assay ID Cell Division and Metabolism BIRC5 Hs04194392_s1 CDCA7 Hs00230589_m1 CDK2 Hs01548894_m1 G0S2 Hs00274783_s1 MCM4 Hs00907398_m1 MKI67 Hs01032443_m1 SAP30 Hs01009154_g1 TYMS Hs00426586_m1 UHRF1 Hs01086727_m1

Survival and Apoptosis BAD Hs00188930_m1 BAK1 Hs00832876_g1 BAX Hs00180269_m1 BCL2L1 Hs00236329_m1 BCL2L11 Hs00708019_s1 BIK Hs00154189_m1 CASP1 Hs00354836_m1 FAS Hs00236330_m1 FASLG Hs00181225_m1 MCL1 Hs01050896_m1 TNFSF10 Hs00921974_m1 TNFSF14 Hs00542477_m1 XAF1 Hs00213882_m1 XIAP Hs00745222_s1

Effector Molecules GZMA Hs00989184_m1 GZMB Hs01554355_m1 GZMK Hs00157878_m1 PROK2 Hs01587689_m1 SPON2 Hs00202813_m1

Transcription Factors BCL6 Hs00153368_m1 EOMES Hs00172872_m1 FOXP3 Hs01085834_m1 GATA3 Hs00231122_m1 LEF1 Hs01547250_m1 NR3C2 Hs01031809_m1 PRDM1 Hs00153357_m1 RORC Hs01076122_m1 SCML1 Hs00232467_m1 SOCS3 Hs02330328_s1 TBX21 Hs00203436_m1 TSC22D3 Hs00608272_m1 ZNF395 Hs00608626_m1 ZNF683 Hs00543184_m1

Migration and Adhesion ADAM8 Hs00174246_m1 CCR1 Hs00928897_s1 CCR5 Hs99999149_s1 CCR7 Hs01013469_m1 CXCR1 Hs01921207_s1 CXCR3 Hs01847760_s1 CXCR5 Hs00540548_s1 CX3CR1 Hs01922583_s1 ITGA6 Hs01041011_m1 ITGAD Hs00236178_m1 ITGAL Hs00158218_m1 ITGB1 Hs00559595_m1 ITGB2 Hs00164957_m1 SELL Hs00174151_m1

Cytokine Receptors CXCR6 Hs01890898_s1 IL2RA Hs00907779_m1 IL2RB Hs01081697_m1 IL7R Hs00902334_m1 IL17RA Hs01064648_m1 IL18R1 Hs00977691_m1 IL28RA Hs00417120_m1

Chemokines and Cytokines CCL4 Hs00605740_g1 CCL5 Hs00174575_m1 CXCL13 Hs00757930_m1 IFNG Hs00989291_m1 IL2 Hs00174114_m1 IL4 Hs00174122_m1 IL8 Hs00174103_m1 IL10 Hs00961622_m1 IL17A Hs00174383_m1 IL21 Hs00222327_m1 IL32 Hs00992441_m1 TGFB1 Hs00998133_m1

Differentiation and Exhaustion BTLA Hs00699198_m1 CD27 Hs00386811_m1 CD28 Hs01007422_m1 CD160 Hs00199894_m1 CD244 Hs00175568_m1 CD40LG Hs00163934_m1 CTLA4 Hs03044418_m1 HAVCR2 Hs00262170_m1 ICOS Hs00359999_m1 KLRC1 Hs00970273_g1 KLRD1 Hs00233844_m1 KLRG1 Hs00929964_m1 LAG3 Hs00158563_m1 PDCD1 Hs01550088_m1 PTGER2 Hs00168754_m1 housekeeping genes 18S Hs99999901_s1 ACTB Hs01060665_g1 B2M Hs00984230_m1