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Cd79a Percp-Cy5.5 Noto Anche Come: Mb-1 Catalog Number(S): 9045-0792-025 (25 Tests), 9045-0792-120 (120 Tests)

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Cd79a Percp-Cy5.5 Noto Anche Come: Mb-1 Catalog Number(S): 9045-0792-025 (25 Tests), 9045-0792-120 (120 Tests) Page 1 of 2 CD79a PerCP-Cy5.5 Noto anche come: mb-1 Catalog Number(s): 9045-0792-025 (25 tests), 9045-0792-120 (120 tests) Profili di fluorescenza di normali linfociti del sangue periferico umano non colorati (istogramma blu) o colorati a livello intracellulare con CD79a coniugati con PerCP-Cy5.5 (istogramma viola). Informazioni sul prodotto Indice: CD79a PerCP-Cy5.5 Storage Conditions: Conservare a 2-8 °C. Catalog Number(s): 9045-0792-025 (25 tests), Non congelare. 9045-0792-120 (120 tests) Materiale fotosensibile. Clone: HM47 Attenzione: contiene azide Concentrazione: 5 µl (0,03 µg)/test (un test viene Manufacturer: eBioscience, Inc., 10255 Science definito come la quantità in grado di colorare Center Drive, San Diego, CA 92121, USA 1x10e6 cellule in 100 μl) Authorized Representative: Bender MedSystems Ospite/isotipo: IgG1 di topo, kappa GmbH, an eBioscience Company Campus Vienna Workshop HLDA: V Biocenter 2 A-1030 Vienna Austria Formulazione: Tampone acquoso, 0,09% di sodio azide; può contenere proteina carrier/stabilizzante. Uso previsto Descrizione L'anticorpo monoclonale HM47 coniugato con fluorocromo L'anticorpo monoclonale HM47 riconosce il dominio reagisce con l'antigene CD79a umano. Il CD79a può essere citoplasmatico del CD79a, chiamato anche mb-1. Il CD79a è rilevato in campioni biologici umani mediante tecniche una glicoproteina di membrana da 47 kDa che si unisce al immunologiche. CD79b con cui forma il recettore eterodimerico per i linfociti Principi del test B (BCR). Questo recettore è responsabile della segnalazione La citometria a flusso è uno strumento utile per la misurazione dei linfociti B e causa la loro attivazione, apoptosi o anergia. simultanea delle diverse proprietà fisiche di singole particelle L'espressione del CD79a si verifica durante tutto lo sviluppo, (come ad es., le cellule). Le cellule attraversano un raggio laser dalle prime cellule pre-linfociti B alle plasmacellule. Il CD79 allineate una dietro l'altra. Per ogni cellula che attraversa il viene espresso sulle neoplasie dei linfociti B e su alcuni raggio laser il citometro registra il modo in cui la cellula o la tumori maligni senza linfociti B, come la leucemia mieloide particella disperdono la luce laser incidente ed emettono acuta. A differenza degli altri anticorpi che possono risultare fluorescenza. Mediante questo protocollo di analisi con nascosti nei linfociti B neoplastici, il HM47 è un anticorpo citometria a flusso è possibile eseguire l'analisi simultanea delle affidabile che permette il riconoscimento del CD79a e di molecole di superficie a livello della singola cellula. conseguenza, è un ottimo marker dei linfociti B. Document # 30778_03_it • Effective date: 1/25/2011 Not for further distribution without written consent. Copyright © 2000-2010 eBioscience, Inc. www.eBioscience.com ORDER INQUIRIES • +43 1 796 40 40-120 TECH • +43 1 796 40 40-0 [email protected] Page 2 of 2 Istruzioni per il prelievo e la conservazione dei campioni 8. Risospendere il pellet di cellule colorate in 1 ml di Flow Prelevare un campione di sangue venoso mediante venipuntura Cytometry Staining Buffer e analizzare i campioni con in una provetta sterile per la raccolta del sangue, utilizzando un un citometro a flusso. anticoagulante appropriato (si consiglia di utilizzare EDTA). Limiti Conservare i campioni a temperatura ambiente (18-25 °C). 1. Per una performance ottimale degli anticorpi coniugati Prima dell'uso, miscelare i campioni agitandoli delicatamente. con il fluorocromo, conservare le fiale al buio a 2-8 °C. Materiali necessari ma non forniti Non congelarle. Provette da 12 x 75 mm 2. Centrifugare la fiala con gli anticorpi per recuperare il Tamponi (si consiglia eBioscience Flow Cytometry massimo volume possibile. Staining Buffer, N. cat. 00-4222) 3. Tranne quando riportato nel protocollo, tutte le Tampone di lisi (si consiglia eBioscience 1X RBC Lysis colorazioni devono essere eseguite su ghiaccio oppure Buffer, N. cat. 00-4333 o eBioscience 1-step Fix/Lyse a 2-8 °C con una minima esposizione alla luce. Solution (10X), N. cat. 00-5333) Caratteristiche della performance Per la colorazione intracellulare utilizzare IC Fixation La costanza della performance dei reagenti di alta qualità Buffer e Permeabilization Buffer, N. cat. 88-8823 viene assicurata mediante l'analisi di ciascun lotto di (colorazione per le citochine intracellulari o per le anticorpi monoclonali per eseguire il confronto tra la loro proteine citoplasmatiche) o Foxp3 Buffer Set, conformità e le caratteristiche di un reagente standard. N. cat. 00-5523 (colorazione delle proteine nucleari). Quando ritenuto idoneo, vengono inclusi i dati Fare riferimento alla sezione Best Protocols del sito rappresentativi della citometria a flusso. web di eBioscience per i protocolli "Staining Segni di deterioramento Intracellular Antigens for Flow Cytometry". Per qualsiasi domanda o dubbio riguardanti la performance Colorazione di vitalità (si consiglia 7-AAD Viability o la qualità dei prodotti ricevuti, contattare l'assistenza Staining Solution, N. cat. 00-6993 o Propidium Iodide tecnica di eBioscience (vedere di seguito). Staining Solution, N. cat. 00-6990) Pipette automatiche Bibliografia Centrifuga Procedures for the Collection of Diagnostic Blood Specimens Miscelatore Vortex by Venipuncture (H3-A6), 3rd Edition published by the Secchio con ghiaccio o frigorifero National Committee for Clinical Laboratory Standards. Citometro a flusso Schlossman SF, Boumsell L, Gilks W, et al, eds. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford Protocollo del test University Press. New York. 1995. NOTA: per la colorazione intracellulare, fare riferimento alla Sakaguchi N, et al. B lymphocyte lineage-restricted sezione Best Protocols del sito web di eBioscience per i expression of mb-1, a gene with CD3-like structural protocolli "Staining Intracellular Antigens for Flow Cytometry". properties. EMBO J. 1988;7:3457-3464. 1. Erogare 100 µl del campione di analisi nelle provette. Nota legale 2. Aggiungere 5 µl dell'anticorpo appropriato in ciascuna FOR RESEARCH (NOT TO INCLUDE IN VIVO RESEARCH) AND/OR IN VITRO DIAGNOSTIC USE ONLY. provetta. THE ONLY PERMITTED COMMERCIAL USE IS PROVISION OF RESEARCH (NOT TO INCLUDE IN VIVO 3. Incubare per 30-60 minuti a 2-8 °C. In alternativa, RESEARCH) SERVICES AND/OR IN VITRO DIAGNOSTIC SERVICES. NOT FOR THERAPEUTIC OR IN i campioni possono essere incubati al buio a VIVO APPLICATIONS. OTHER USE NEEDS LICENSES WHICH ARE AVAILABLE FROM GE HEALTHCARE temperatura ambiente per 15-30 minuti. BIO-SCIENCES CORP. UNDER U.S. PATENT NOS. NOS 5,268,486, 5,569,587 AND 5,627,027 AND 4. Aggiungere 2 ml di 1X RBC Lysis Buffer (a temperatura FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. ambiente) ad ogni provetta. Miscelare delicatamente. THIS PRODUCT IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES (In alternativa, è possibile incubare i campioni con 2 ml CORP. ("GE HEALTHCARE") AND CARNEGIE MELLON UNIVERSITY ("CMU") AND MADE AND di 1-step Fix/Lyse Solution). SOLD UNDER LICENSE FROM GE HEALTHCARE. THE PRODUCT BEARING THIS LABEL IS 5. Incubare i campioni al buio e a temperatura ambiente LICENSED FOR SALE ONLY FOR RESEARCH (NOT TO INCLUDE IN VIVO RESEARCH) AND IN per 10 minuti. Con l'RBC Lysis Buffer non superare ADDITION MAY BE USED TO PERFORM RESEARCH (NOT TO INCLUDE IN VIVO RESEARCH) 15 minuti di incubazione. AND/OR IN VITRO DIAGNOSTIC SERVICES FOR THIRD PARTIES. IT IS NOT LICENSED NOR ARE 6. Centrifugare i campioni a 300-400 x g per 5 minuti a THERE ANY IMPLIED LICENSES FOR ANY OTHER USE INCLUDING, WITHOUT LIMITATION, temperatura ambiente, decantare/aspirare il surnatante e COMMERCIAL USES UNLESS EXPRESSLY AUTHORIZED BY GE HEALTHCARE IN WRITING. IF lavare 1 volta con 2 ml di Flow Cytometry Staining Buffer. YOU INTEND TO USE THIS PRODUCT COMMERCIALLY AND YOU DO NOT HAVE A LICENSE 7. Centrifugare i campioni a 300-400 x g per 5 minuti a TO USE THIS PRODUCT FOR THE INTENDED COMMERCIAL PURPOSE, RETURN THIS temperatura ambiente, decantare/aspirare il surnatante. PRODUCT, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE PRODUCT WILL BE REFUNDED. Document # 30778_03_it • Effective date: 1/25/2011 Not for further distribution without written consent. Copyright © 2000-2010 eBioscience, Inc. www.eBioscience.com ORDER INQUIRIES • +43 1 796 40 40-120 TECH • +43 1 796 40 40-0 [email protected] .
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