https://doi.org/10.5194/bg-2020-392 Preprint. Discussion started: 9 November 2020 c Author(s) 2020. CC BY 4.0 License. 1 Carbon storage in phosphorus limited grasslands may 2 decline in response to elevated nitrogen deposition: a long- 3 term field manipulation and modelling study 4 Christopher R. Taylor1, Victoria Janes-Bassett3, Gareth Phoenix1, Ben Keane1, Iain P. Hartley2, Jessica 5 A.C. Davies3 6 1Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK 7 2Geography, College of Life and Environmental Science, University of Exeter, Exeter, UK 8 3Lancaster Environment Centre, Lancaster University, Lancaster, UK 9 10 Corresponding author: Christopher Taylor (
[email protected]) 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Page 1 of 37 https://doi.org/10.5194/bg-2020-392 Preprint. Discussion started: 9 November 2020 c Author(s) 2020. CC BY 4.0 License. 25 Abstract 26 In many temperate ecosystems, nitrogen (N) limits productivity, meaning anthropogenic N 27 deposition can stimulate plant growth and subsequently carbon (C) sequestration. Phosphorus 28 (P) and N-P co-limited grasslands are widespread, yet there is limited understanding of their 29 responses to N deposition, which may transition more ecosystems toward P-limited or N-P co- 30 limited states. Here, we investigate the consequences of enhanced N addition on the C-N-P 31 pools of grasslands in different states of nutrient limitation. We explored the response of a long- 32 term nutrient-manipulation experiment on two N-P co-limited grasslands; an acidic grassland of 33 stronger N-limitation and a calcareous grassland of stronger P-limitation, by combining data with 34 an integrated C-N-P cycling model (N14CP).