Mitochondrial DNA, August 2012; 23(4): 290–292

MITOGENOME ANNOUNCEMENT

Complete mitochondrial genome sequence of the Callionymus curvicornis (: Callionymoidei: Callionymidae)

HA YEUN SONG1, TAKASHI P. SATOH2, & KOHJI MABUCHI1

1Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8564, Japan, and 2National Museum Nature and Science, Collection Center, 4-1-1 Amakubo, Tsukuba City, Ibaraki 305-0005, Japan

(Received 17 February 2012; revised 20 February 2012; accepted 6 March 2012)

Abstract We determined the complete mitochondrial genome (mitogenome) sequence of the dragonet Callionymus curvicornis. The total length of C. curvicornis mitogenome is 16,406 bp, which consists of 13 protein–coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. It has the typical vertebrate mitochondrial gene arrangement. This is the first report of a complete mitochondrial genome in the fish suborder Callionymoidei.

Keywords: Repomucenus curvicornis, Repomucenus richardsonii, Percomorpha, mitogenome

The suborder Callionymoidei is a group of small mitogenome sequence of an important food species

For personal use only. benthic marine fishes primarily occurring in Indo- called “Nezumi-gochi” in Japanese, as the first West Pacific. Two families make up the suborder, complete mitogenome sequence of the suborder the callionymid (182 species) and the Callionymoidei. For this species, multiple scien- deeper water draconettid slope dragonets (12 species) tific names are used: Callionymus curvicornis and (Nelson 2006). The former family (Callionymidae) Repomucenus curvicornis are usually used now, but includes some important food species in Japan, as well an old name, Repomucenus richardsonii still appears as a worldwide popular aquarium species, Synchiropus sometimes (for details, see Nakabo 2002). splendidus. The taxonomic and phylogenetic studies Total genomic DNA was extracted from an ethanol- within Callionymidae were intensively performed by preserved specimen of “Nezumi-gochi” (NSMT- Nakabo (1982, 1983a,b) and Fricke (1983, 2002) P96286, collected from Seto Inland Sea of Hiroshima based on morphological characters. Their phylo- Prefecture, Japan). The mitogenome was amplified in genetic position within the Series Percomorpha is, its entirety using a long PCR technique (Cheng et al. Mitochondrial DNA Downloaded from informahealthcare.com by The University of Manchester on 12/09/14 however, still unclear, although affinity with gobieso- 1994). We used fish-versatile PCR primers in various cids has been proposed (Gill 1996; Springer combinations to amplify contiguous, overlapping and Johnson 2004). In many recent studies, whole segments of the entire mitogenome. All the exper- mitochondrial genome (mitogenome) sequences has iments were carried out following protocols previously been demonstrated as being useful for resolving higher described (Miya and Nishida 1999). The complete level relationships of percomorph fishes. But, no mitogenome of this specimen was 16,406 bp in length, complete sequence has been reported from this being deposited in DDBJ/GenBank/EMBL with suborder. In this study, we determined the complete accession number: AP012307. The mitogenome

Correspondence: H. Y. Song, Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8564, Japan. Tel: þ 81 4 7136 6215. Fax: þ 81 4 7136 6216. E-mail: [email protected]

ISSN 1940-1736 print/ISSN 1940-1744 online q 2012 Informa UK, Ltd. DOI: 10.3109/19401736.2012.674124 Mitochondrial genome of C. curvicornis 291

Table I. Mitochondrial genome characteristics of C. curvicornis.

Position Codon Intergenic Gene From To Length (bp) Start Stopnucleotides* Strand†

tRNAPhe 16868 2 H 12S rRNA 69 1011 943 0 H tRNAVal 1012 1082 71 0 H 16S rRNA 1083 2742 1660 0 H tRNALeu(UUR) 2743 2815 73 0 H ND1 2816 3787 972 ATG TAA 4 H tRNAIle 3792 3861 70 0 H tRNAGln 3862 3933 72 21L tRNAMet 3933 4002 70 0 H ND2 4003 5048 1046 ATG TA 0 H tRNATrp 5049 5119 71 0 H tRNAAla 5120 5188 69 1 L tRNAAsn 5190 5262 73 32 L tRNACys 5295 5358 64 0 L tRNATyr 5359 5425 67 1 L COI 5427 6971 1545 GTG TAA 3 H tRNASer(UCN) 6975 7045 71 3 L tRNAAsp 7049 7117 69 3 H COII 7121 7810 690 ATG TAA 0 H tRNALys 7811 7885 75 1 H ATP8 7887 8051 165 ATG TAG 27H ATP6 8045 8727 683 ATG TA 0 H COIII 8728 9512 785 ATG TA 0 H tRNAGly 9513 9581 69 0 H ND3 9582 9930 349 ATG T 0 H tRNAArg 9931 9999 69 0 H ND4L 10,000 10,296 297 ATG TAA 27H ND4 10,290 11,663 1374 ATG TAA 2 H tRNAHis 11,666 11,734 69 1 H tRNASer(AGY) 11,736 11,802 67 2 H tRNALeu(CUN) 11,805 11,877 73 3 H ND5 11,881 13,719 1839 ATG TAA 24H For personal use only. ND6 13,716 14,234 519 ATG TAG 0 L tRNAGlu 14,235 14,302 68 3 L Cytb 14,306 15,446 1141 ATG T 0 H tRNAThr 15,447 15,517 71 21H tRNAPro 15,517 15,585 69 0 L CR 15,586 16,406 821 0 2

* Numbers correspond to the nucleotides separating different genes. Negative numbers indicate overlapping nucleotides between adjacent genes; † H and L denote heavy and light strands, respectively.

consists of 13 protein-coding genes, 22 tRNA genes, with complete stop codons TAA or TAG. Control and 2 rRNA genes as listed in Table I. Eight tRNA region (CR) is located between tRNAPro and and ND6 genes are encoded on the light strand. tRNAPhe, and 821 bp long. Mitochondrial DNA Downloaded from informahealthcare.com by The University of Manchester on 12/09/14 The overall base composition of the heavy strand is The present result provides the first foothold for 26.87% A, 27.55% C, 17.73% G, and 27.84% T. The the mitogenomic analysis of the phylogenetic position AT content is higher than the GC contents as shown of the suborder Callionymoidei within the Series in other vertebrate mitogenomes (Saccone et al. Percomorpha. In addition, it can also contribute to 1999). All tRNA genes can fold into a typical elucidate the population genetic structure of this cloverleaf structure, with length ranges from 64 to important food species in East Asia. 75 bp. The 12S rRNA and 16S rRNA genes are located between tRNAPhe and tRNAVal (943 bp) and between rRNAVal and tRNALeu(UUR) (1660 bp), Acknowledgements respectively. The majority of protein-coding genes (12 of 13 genes) start with ATG, whereas COI starts We heartily appreciate Center for Molecular Bio- with GTG. While 5 of the 13 protein-coding genes diversity Research (NSMT) for providing a specimen were shown to terminate with incomplete of C. curvicornis (NSMT-P96286). This work was stop codons, T (ND3 and Cytb)andTA(ND2, supported by JSPS Research grants (19207007, ATP6, and COIII), the remaining eight genes ended 23370041). 292 H. Y. Song et al.

Declaration of interest: The authors report no Stomiiformes): First example of transfer RNA gene rearrange- conflict of interest. The authors alone are responsible ments in bony fishes. Mar Biotechnol 1:416–426. for the content and writing of the paper. Nakabo T. 1982. Revision of the genera of dragonets (Pisces: Callionymidae). Publ Seto Mar Biol Lab 27:77–131. Nakabo T. 1983a. Revision of dragonets (Pisces: Callionymidae) References found in the Waters of Japan. Publ Seto Mar Biol Lab 27: 193–259. Cheng S, Higuchi R, Stoneking M. 1994. Complete mitochondrial Nakabo T. 1983b. Comparative osteology and phylogenetic genome amplification. Nat Genet 7:350–351. relationships of the dragonets (Pisces: Callionymidae) with Fricke R. 1983. Revision of the Indo-Pacific genera and species of some thoughts of their evolutionary history. Publ Seto Mar Biol the dragonet family Callionymidae (Teleostei). Theses Zoologi- Lab 28:1–73. cae 3:x þ 774. Nakabo T. 2002. Callionymidae. In: Nakabo T, editor. Fishes of Fricke R. 2002. Annotated checklist of the dragonet families Japan with pictorial keys to the species. English ed., Tokyo: Tokai Callionymidae and (Teleostei: Callionymoidei), University Press, 1125–1138, 1594–1595. with comments on callionymid fish classification. Stuttgarter Nelson JS. 2006. Fishes of the World. 4th ed., New York: John Wiley Beitra¨ge zur Naturkunde Serie A (Biologie) 645:1–103. and Sons, Inc. Gill AC. 1996. Comments on an intercalar path for the glossopharyngeal (cranial IX) nerve as a synapomorphy of Saccone S, Giorgi CD, Gissi C. 1999. Evolutionary genomics in the Paracanthopterygii and on the phylogenetic position of Metazoa: The mitochondrial DNA as a model system. Gene the Gobiesocidae (Teleostei: Acanthomorpha). Copeia 4: 238:195–209. 1022–1029. Springer VG, Johnson GD. 2004. Text 260 and plates 205 Study of Miya M, Nishida M. 1999. Organization of the mitochondrial the dorsal gill-arch musculature of teleostome fishes, with special genomeofadeep-seafish,Gonostoma gracile (Teleostei: reference to the . Bull Bio Soc Wash 11. For personal use only. Mitochondrial DNA Downloaded from informahealthcare.com by The University of Manchester on 12/09/14 本文献由“学霸图书馆-文献云下载”收集自网络，仅供学习交流使用。

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