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Survey for the Incidence of Tobacco Streak Virus (TSV) in Field and Horticultural Crops

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Survey for the Incidence of Tobacco Streak Virus (TSV) in Field and Horticultural Crops Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 3657-3669 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.710.423 Survey for the Incidence of Tobacco streak virus (TSV) in Field and Horticultural Crops M. Sunil Kumar1*, R. Sarada Jayalakshmi Devi2, M. Krishna Reddy3, K. Vemana4, T. Murali Krishna5 and L. Prasanthi6 1Department of Plant Pathology, S.V. Agricultural College, Tirupathi, 517 502, India 2University Librarian, Acharya N.G. Ranga Agricultural University, Guntur, 522 209, India 3Division of Plant Pathology, ICAR-IIIHR, Bangalore, 560089, India 4Department of Plant Pathology, Agricultural Research Station, Kadiri, India 5Department of Entomology, 6Department of genetics & Plant Breeding, IFT, RARS, Tirupathi, 517 502, India *Corresponding author ABSTRACT Survey conducted for disease incidence of Tobacco streak virus (TSV) during Kharif K e yw or ds 2014-15 and 2015-16 in Andhra Pradesh and Karnataka revealed disease incidence of 9-28 per cent in groundnut, 6-18 per cent in sunflower and 5-22 per cent in cucumber. In Ilarvirus , TSV, Okra, Gherkin, Cucumber, Andhra Pradesh during Kharif 2014-15 and 2015-16, the incidence of TSV in groundnut Pumpkin ranged from 9.8 (Bathalapalli) to 28.2 (Obuldevarayacheruvu) per cent and 9.2 (Kothacheruvu) to 23.2 (Gorantla) per cent respectively; similarly on sunflower the Article Info incidence ranged from 6.4 (Aluru) to 18.7 (Bethamcherla) per cent and 5.2 (Adoni) to 14.2 (Bethamcherla) per cent respectively. In Karnataka the incidence of TSV during Kharif Accepted: 26 September 2018 2014-15 and 2015-16 ranged from 10.9 (Herapanahalli) to 22.8 (Tumkur) per cent and 5.2 Available Online: (Pavagada) to 14.2 (Tumkur) per cent respectively. The collected samples were tested by 10 October 2018 Enzyme Linked Immunosorbent Assay (ELISA) using TSV specific antisera and through electron microscopy and positive samples were maintained on cowpea for further studies. Introduction gherkin, cucumber, pumpkin) has been seriously hampered due to TSV infection. Due TSV is the type member of Ilarvirus genus of to wide range of host plants, transmission the Bromoviridae family. In recent years, through pollen and seed, this virus was placed Ilarviruses (TSV) had received national and among the most economically important virus. international attention because they cause TSV infection at seedling stage results in major crop losses. TSV had caused severe premature death of the plant, Infection during epidemics in India on several important crops mid – stage of the plant growth may result in such as groundnut, sunflower, okra, cotton, necrosis of the leaves and severe reduction in cucurbits and gherkins. However, in recent yield. Infection at late stage of the plant years, vegetable crop cultivation (okra, growth results in mild chlorotic symptoms 3657 Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 3657-3669 with little effect on plant growth and yield. In allowed to stay for 2-3 minutes. The excess several weed hosts, such as parthenium, TSV stain was drained by touching the blotting causes asymptomatic infection. Keeping in paper strip to the edge of the grid. The grids view the economic importance of virus, a were dried for 15-30 min in dessicator and survey was conducted in Andhra Pradesh and examined under JOEL 100 S transmission Karnataka to assess the disease incidence of electron microscope at various magnifications. TSV in groundnut, sunflower and cucumber. The photographs of the virus particles were taken. Materials and Methods Actual size of a Particle in nm = Field surveys Size of the particle in electron micrograph (mm) Magnification Roving survey was conducted to note the incidence of Tobacco Streak Virus (TSV) in Bioassay different crop plants viz., groundnut, sunflower and Cucumber from different Suspected TSV infected samples from locations of Andhra Pradesh and Karnataka. groundnut, sunflower and cucumber were The Suspected TSV infected samples collected from different locations of Andhra exhibiting characteristic symptoms of severe Pradesh and Karnataka, were used for sap necrosis of leaf lamina, petioles and other inoculation on cowpea (Vigna unguiculata cv. plant parts were collected and identified by C-152), a diagnostic host for TSV. Test plants Enzyme Linked Immunosorbent Assay were raised in insect proof glasshouse (ELISA) using TSV specific antisera or conditions. Five to eight seeds were planted in through electron microscopy by leaf dip each plastic pot (4” diameter) and method. The TSV identified samples either o cotyledonary stage with uniform growth were stored in -80 C for further virus selected for inoculation. Infected as well as characterization or mechanically inoculated to healthy tissues from field samples macerated test hosts for culture maintenance in an insect separately using sterilized chilled pestle and proof glass house. motor in 0.1M phosphate buffer (pH 7.2, 1:1 w/v) containing 0.1% mercaptoethanol as Electron microscopy extraction buffer (EB) (Appendix I). The sap was kept on ice till the inoculation was The suspected plant sample was ground in completed. The test plant seedlings were Sodium phosphate buffer pH 7.5 at 1:5 (w/v) dusted with celite (diatomaceous earth) or proportion filtered through double layered carborandum powder, which served as an cheese cloth and observed under transmission abrasive. The inoculum was applied directly electron microscope for determining the shape on to the upper surface of the leaves. After and size of the virus particles causing mosaic inoculations, the seedlings were sprayed with symptoms on diseased sample. To examine distilled water and kept in the insect-proof virus particles in suspected leaf, a drop of glass house. Inoculations were preferably filtered leaf preparation was placed on the carried out in the evening. The inoculated carbon-coated grids and allowed to settle for seedlings were observed for symptoms 2-3 minutes. The excess of sample was development. Local lesions developed 4-5 removed by using blotting paper. A small days post-inoculation were used for further droplet of dye (uranyl acetate or inoculation by taking single lesion and phosphotungstic acid) was placed on it and subsequently maintained pure virus cultures of 3658 Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 3657-3669 groundnut, sunflower and cucumber TSV cent-2004; 5-80 per cent-2006 and 2-30 per isolates on cowpea. cent-2007) in Andhra Pradesh (Anonymous 2004b, 2006 and 2007). TSV incidence has Results and Discussion also been reported on groundnut in Raichur district of Karnataka (Prasada Rao et al., Intensive roving survey was conducted during 2003). Sunflower Necrosis Disease (SND) Kharif 2014-15 and 2015-16 to record the caused by Tobacco streak virus was reported disease incidence of TSV in groundnut, as an epidemic consecutively for the three sunflower and cucumber at different places in years (1997-99), with the incidence ranging Andhra Pradesh and Karnataka. The incidence from 10 to 80 percent and causing yield losses of TSV in Andhra Pradesh ranged from 9-28 up to 90 per cent in most of the sunflower per cent in groundnut and 6-18 per cent in growing regions of Southern India (DOR sunflower samples. The incidence of TSV in Annual Report, 2001). Halakeri (1999) cucumber ranged from 5-22 percent in recorded incidence of sunflower necrosis Tumkur and Davanagere districts of disease ranged from 3 to 70 per cent around Karnataka. In Andhra Pradesh during Kharif Dharwad, Gadag, Bagalkot, Haveri and 2014-15 the incidence of TSV in groundnut Bijapur districts. Five to thirty per cent was highest in Obuldevaraya cheruvu (28.2 necrosis disease was recorded at Sira, per cent) and lowest in Bathalapalli (9.8 per Chitradurga, Bellary, Bijapur and Gulbarga cent). Similarly Gorantla recorded highest districts, 50 to 70 per cent around Nagalapur disease incidence of 23.2 per cent and and Lingsur of Raichur district (Anonymous, Kothacheruvu recorded lowest disease 2002). Krishna Reddy et al., (2003) reported incidence of 9.2 per cent during Kharif 2015- severe outbreak of Tobacco streak virus 16 (Table 1 and Fig. 1). (TSV) in cucumber and gherkin causing yield losses of 31 to 75 per cent in Bangalore, In sunflower, Bethamcherla recorded highest Bellary, Davanagiree, and Tumkur districts of disease incidence of 18.7 and 14.2 per cent Karnataka state during 2000-2002. during Kharif 2014-15 and 2015-16 respectively and lowest disease incidence was Identification of TSV isolates recorded in Aluru (6.4 per cent) and Adoni (5.2 per cent) during Kharif 2014-15 and Suspected Tobacco streak virus (TSV) 2015-16 respectively (Table 2 and Fig. 2). In infected groundnut samples showing necrotic Karnataka, cucumber growing areas of symptoms on leaves, top bud and stem Tumkur district, Sira recorded highest disease collected from farmer’s fields of different incidence of 22.8 (Kharif, 2014-15) and 14.2 mandals in Anantapur district during Kharif, (Kharif, 2015-16) per cent, while 2014-15 and 2015-16 were tested by bio assay Herapanahalli (10.9 per cent) and Pavagada and ELISA for the TSV identification. Out of (5.2 per cent) recorded lowest disease 44 samples collected, 30 samples reacted incidence during Kharif 2014-15 and 2015-16 positively with polyclonal antiserum to TSV respectively (Table 3 and Fig. 3). by DAC-ELISA. The absorbance values (A405 nm) ranged from 0.19-3.16 (Table 1). TSV TSV incidence was first observed on suspected symptomatic groundnut samples groundnut during the year 2000 (Reddy et al., were sap transmitted to the cowpea seedlings 2002) in Anantapur district of Andhra (Vigna unguiculata cv. 132) and presence of Pradesh. Since then its incidence on groundnut TSV was confirmed by DAC-ELISA (A405 crop has been recorded regularly (16.5 per nm: 2.45-3.12) (Table 4).
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