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DR. BABASAHEB AMBEDKAR MARATHWADA UNIVERSITY, AURANGABAD SYLLABUS of M.Sc

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DR. BABASAHEB AMBEDKAR MARATHWADA UNIVERSITY, AURANGABAD SYLLABUS of M.Sc DR. BABASAHEB AMBEDKAR MARATHWADA UNIVERSITY, AURANGABAD SYLLABUS Of M.Sc. II (Semester III and IV) (Forensic Science) Effective from Academic Year 2013-2014 onwards 1 Government Institute Of Forensic Science, Aurangabad M.Sc. II Year (Forensic Biology, Serology and DNA Finger Printing) Preamble M.Sc.-II (Sem-III & IV) (Forensic Science) Ordinance ------------:- Title of the Program: - M.Sc.-II (Sem-III & IV) (Forensic Science) Ordinance ------------:-- Eligibility: - M.Sc.-I (Forensic Science) Regulation no. ----------- : Specializations :- Four Specializations viz. Finger print and Questioned Document, Forensic Chemistry and Toxicology, Forensic Biology, Serology and DNA Finger Printing, Cyber Space, IT Security and Cyber Forensic may be offered subject to the availability of students as mentioned in the preceding Para/ regulation. Regulation no. -----------:- Minimum intake capacity for each specialization: - There shall be minimum 25% of the intake capacity of the students for each specialization. Regulation no. ----------- :-Allotment of specialization :- The specialization to the students will be allotted on the basis of choice and merit (M.Sc.-I) of the students. However, if the criterion of minimum intake capacity for a particular specialization as mentioned above is not full filled, in such case the students will be diverted to other specialization strictly based on the marks obtained by him/her at M.Sc.-I examination. In such situation the decision of the Head of the concerned Institution shall be final. Regulation no.-------------- :- Course structure Each semester will have four theory papers and two theory based practical papers. In the fourth semester students will carry out Dissertation instead of one practical paper. Each paper shall be of 75 marks. Total Marks for M. Sc.-II (Forensic Science), III and IV Semester Theory (4 Papers per Practical/ Dissertation Total semester each of 75marks) (2 Papers per semester Semester each of 75 marks) Marks Marks Marks III 300 150 450 IV 300 150 450 Total 600 300 900 2 Government Institute Of Forensic Science, Aurangabad M.Sc. II Year (Forensic Biology, Serology and DNA Finger Printing) Specialization-III : Forensic Biology, Serology and DNA fingerprinting Semester – III Paper code Title of the paper Hours per Marks week MFSBS 301 Bioinstrumentation 04 75 MFSBS 302 Biostatistics and Research methodology 04 75 MFSBS 303 Eukaryotic genetics and DNA fingerprinting 04 75 MFSBS 304 Enzymology, Serology & bioinformatics 04 75 MFSBS 305 Practical- I 04/Batch 75 MFSBS 306 Practical –II 04/Batch 75 Total 24 450 Semester -IV Paper code Title of the paper Hours per Marks week MFSBS 401 Forensics in botany, entomology, wildlife and 04 75 environment MFSBS 402 Forensic microbiology and quality assurance 04 75 MFSBS 403 Anthropology and forensic medicine 04 75 MFSBS 404 DNA Profiling and interpretation 04 75 MFSBS 405 Practical- III 04/Batch 75 MFSBS 406 Dissertation 04/Batch 75 Total 24 450 3 Government Institute Of Forensic Science, Aurangabad M.Sc. II Year (Forensic Biology, Serology and DNA Finger Printing) Syllabus Semester - III Paper- I (MFSBS 301): Bioinstrumentation Hrs. /Week-4 Marks: 75__ Unit I:Principle, working and forensic applications of different types of microscopes:light, Fluorescence, Comparison microscope, Phase contrast microscope, Stereoscopic microscope, Polarizing microscope, Fluorescent microscopy, Infra-red microscopy, Scanning Electron Microscope (SEM) & Transmission Electron Microscope (TEM), Laser scanning confocal microscopy, Differential interference microscopy, Atomic force microscope Unit II: Spectrophotometry: Ultra violet and visible spectrophotometry: Types of sources, wavelength selection, filters-cells and sampling devices, detectors, resolution, qualitative and quantitative methods for detection, Fluorescence and phosphorescence spectrophotometry, Atomic absorption spectrometry, Atomic emission spectrometer, X-ray spectroscopy, Infrared spectrophotometry, Mass spectrophotometer, NMR and ESR spectroscopy, Molecular structure determination using X-ray diffraction, NMR, and surface plasma resonance methods and their applications in forensic biology Unit III: Chromatography: General principles, applications and forensic significance of following types of chromatography, paper chromatography, column chromatography, Thin Layer Chromatography (TLC), adsorption chromatography, partition chromatography, gas chromatography, gas-liquid chromatography, ion-exchange chromatography, exclusion (permeation) chromatography, affinity chromatography, HPLC, HPTLC, capillary chromatography, UPLC. Unit IV: Electrophoresis: Theory and general principles, Various factors affecting electrophoresis, low and high voltage electrophoresis, horizontal and vertical Electrophoresis. Various electrophoretic techniques – Sodium dodecyl sulphate (SDS), Agarose Gel Electrophoresis (AGE), Polyacrylamide Gel Electrophoresis (PAGE), Iso-electric focusing (IEF), Gel immuno-diffusion, complement fixation, radio immuno assay (RIA), ELISA, and fluorescence immunoassay. Serological methods for detection and quantitation of viruses including Hepatitis, Influenza, HIV and others, Immuno-assays: SRID, ELISA, ELISA-PCR, RIA, western blotting, immunofluorescence and their applications. Immune-deficiencies (e.g. Rheumatoid arthritis) and autoimmunity, detection of genetic disease by electrophoresis Unit V: Centrifugation: Preparative: Differential centrifugation, Density gradient centrifugation: Rate-Zonal, Isopycnic. Types of centrifuge machines; preparative and analytical centrifuges; differential centrifugation, sedimentation velocity, sedimentation equilibrium, density gradient methods and their applications, ultra centrifugation 4 Government Institute Of Forensic Science, Aurangabad M.Sc. II Year (Forensic Biology, Serology and DNA Finger Printing) *** Suggested reading: 1. Instrumental Methods of Analysis6th Edition. (1986): H.H. Willard, L.L. Merritt Jr. and others.CBS Publishers and Distributors. 2. Instrumental Methods of Chemical Analysis. (1989):Chatwal G and Anand, S. Himalaya Publishing House, Mumbai. 3. A Biologists Guide to Principles and Techniques of Practical Biochemistry. (1975): Williams, B.L. and Wilson, K. 4. Spectroscopy. (Vol. 1): Edited by B.B. Straughan and S. Walker. Chapman and HallLtd. 5. Gel Electrophoresis of Proteins- A Practical Approach: Hanes. 6. Chromatography: Concepts and Contrasts- 1988 by James Miller. John Wiley and Sons. Inc., New York. 7. Analytical Biochemistry: Holme. 8. Introduction to High Performance Liquid Chromatography: R. J. Hamilton and P. A.Sewell. 9. Spectroscopy: B.P. Straughan and S. Walker. 10. Practical aspects of Gas Chromatography and Mass Spectrometry (1984) by Gordon M.Message, John Wiley and Sons, New York. 11. Gel Chromatography by Tibor Kremmery. 12. Principles and Techniques of Biochemistry and Molecular Biology: Edt. Keith Wilson, John Walker *** Paper-II (MFSBS 302): Biostatistics and research methodology Hrs. /Week-4 Marks: 75____ Unit I: Statistical methods: Basic definitions and applications. Sampling: Representative sample, sample size, sampling bias and sampling techniques. Data collection and presentation: Types of data, methods of collection of primary and secondary data. Methods of data presentation-graphical representation by histogram, polygon, ogive curves and pie diagram Unit II: Measures of central tendency: Mean, Median, Mode; Measures of variability: standard deviations, standard error, range, mean deviation and coefficient of variation. Correlation and regression: Positive and negative correlation and calculation of Karl-Pearsons co-efficient of correlation. Linear regression and regression equation and multiple linear regression, ANOVA, one and two way classification 5 Government Institute Of Forensic Science, Aurangabad M.Sc. II Year (Forensic Biology, Serology and DNA Finger Printing) Unit III: Tests of significance: Small sample test (Chi-square, t test, and F test), large sample test (Z test) and standard error. Introduction to probability theory and distributions, (concept without deviation) binomial, poison and normal (only definitions and problems) Unit IV:Research methodology: Meaning of research in biological sciences; Process of research; Identification and criteria of selecting a research problem (Hypothesis); Formulation of objectives; Research plan and its components; Methods of research and difficulties in biological research; Research proposal and experimental design: Key elements- Objective, Introduction, design or rationale of work, Guidelines for design of experiments, material and methods, designing biological experiments, compilation and documentation of data; Major organizations and laboratories related to forensic science in India. A brief idea about government research agencies such as DBT, DST, ICMR, CSIR, UGC, BPR&D, DRDO etc. Unit V: Writing and presentation: Format of research paper and report writing, Procedure of Reference Citation; Significance of writing research papers and review articles; Major Scientific publishers; Impact factor and citation index; Ethics and scientific conduct, Ethics in human and animal studies; Intellectual Property right and Plagiarism; Effective presentation of research findings *** Suggested reading: 1. Statistics in biology, (1967) Vol. 1: Bliss, C.I.K. McGraw Hill, NewYork. 2. Practical Statistics for experimental biologist (1985): Wardlaw, A.C. 3. Statistical Methods in Biology (2000): Bailey, N.T. J. English Univ. Press. 4. Biostatistics - 7th
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