Product Specifications

20,000 detection improved and The optional HCD collision cell adds additional functionality the benchtop to system providing by all-ion fragmentation (AIF) for compound confirmation, while maintaining high-resolution, accurate-mass sensitivity. performance high at The Exactive Plus benchtop system with full-scan and AIF modes is ideally suited for food safety and environmental analysis, research, clinical and toxicology forensic as as well drug metabolism studies. With an optional extended mass range (EMR) of up to m/z of high-mass ions, the Exactive Plus EMR is an outstandingsystem platform for biopharmaceutical research biological and antibodies, monoclonal of analysis requiring antibody drug conjugates, assemblies, protein and other proteins their in native states.

™ Plus ™

Exactive ™

technology. Advanced signal ™ 200 distinguishes target compounds Orbitrap The Thermo Scientific benchtop LC-MS provides high-resolution, accurate-mass (HR/AM) full-scan mass spectra Thermo proven using Scientific processing allows scan Hz speeds up to 12 analyses quantitative and screening for compatible with fast UHPLC chromatography. Mass resolution up to 140,000 (FWHM) at m/z from interferences, analysis of enabling demanding samples matrices. complex in The Exactive Plus mass spectrometer is the ultimate solution for screening applications, from routine compound identification the to trace-level analysis of most challenging compounds complex in mixtures. Fast polarity switching between positive and negative scan modes saves time allowing by complete screening analyses to be completed a in mass high resolutionsingle run. True data need the reduces and positives false avoids reanalyzeto samples. Benchtop Full-Scan Orbitrap Orbitrap Benchtop Full-Scan Mass Spectrometers Exactive Plus & Exactive Plus EMR

Thermo ScientificThermo MASS SPECTROMETRY MASS In-source CID (collision-induced In-source dissociation) collisional HCD (higher-energy optional) dissociation, Routine sub-ppm mass accuracy Routine sub-ppm mass of up to 140,000 Resolving power (FWHM) at m/z 200 Maximum scan speed of 12 Hz of range dynamic Intrascan > 5,000:1 of 50 to 6,000 m/z Mass range mode) or 350 to 20,000 m/z (standard (EMR mode) Full-scan MS analysis with multiple All-ion fragmentation dissociation techniques: - - Onsite upgradable from Exactive Plus from Exactive Onsite upgradable mass spectrometer to Q Exactive mass spectrometer S-Lens for increased sensitivity S-Lens for increased • • • • • • • • • 2 Hardware Specification Vacuum System Performance Characteristics Thermo Scientific Ion Max API Source • Differentially pumped vacuum system with Mass range -9 • Enhanced sensitivity and ruggedness final vacuum <1× 10 mbar Standard mode m/z 50–6,000 EMR mode m/z 350–20,000 • Reduced chemical noise upon sweep gas • Two split-flow turbomolecular pumps and application one rotary vane pump Resolving power Up to 140,000 (FWHM) @ m/z 200 • 60° interchangeable ion probe orientation • Seven vacuum regions Max scan rate Up to 12 Hz at mass • Removable metal ion transfer capillary Orbitrap Mass Analyzer • Nitrogen- filled curved linear trap (C-Trap) resolution setting of provides vent-free maintenance 17,500 @ m/z 200 • Highly efficient ion transfer to Orbitrap • H-ESI II probe with dual desolvation zone Mass accuracy1 analyzer technology Standard Internal: <1 ppm RMS Ion Optics • Orbitrap analyzer with 5 kV central External: <3 ppm RMS electrode voltage • S-Lens stacked-ring radio frequency (RF) EMR mode External: <3 ppm RMS ion guide captures and efficiently focuses • Low-noise image current pre-amplifier for m/z 350–6000 the ions into a tight beam. Large variable • 16-bit signal digitalization External: <5 ppm RMS spacing between electrodes allows for Data Acquisition for m/z 6001–10,000 better pumping efficiency and improved • Ultrafast real-time data acquisition and External: <10 ppm RMS ruggedness instrument control system for m/z 10,001–15,000 • Advanced ion guides for high sensitivity • Fully automated calibration via instrument Sensitivity In full-scan MS mode: and ruggedness control software 500 fg buspirone • High stability and ion transmission on-column S/N • Automated gain control efficiency 100:1 acquired with H-ESI II probe Dynamic range >5000:1 in single scan, intrascan dynamic range Polarity switching One full cycle in <1 sec (one full positive mode scan and one full negative mode scan at mass resolution setting of 35,000 @ m/z 200) HCD Cell C-Trap Octopole Analog inputs One analog input (0 –1 V) One analog input (0 –10 V)

1. Under defined conditions Options • Axial higher-energy collisional dissociation (HCD) cell • Extended mass range (EMR) of S-Lens 350–20,000 m/z with software control of HCD collision gas pressure for enhanced Orbitrap analysis of native-state proteins Mass Analyzer • Thermo Scientific Nanospray Flex ion source – single set-up for all online nanoflow applications • ESI probe compatible with liquid flow Schematic of the Thermo Scientific Exactive Plus and Exactive Plus EMR Benchtop LC-MS systems rates of <1 μL/min to 1 mL/min without splitting • APCI source compatible with liquid flow rates of 50 μL/min to 2 mL/min without splitting • APCI/APPI source compatible with liquid flow rates of 50 μL/min to 2 mL/min without splitting • The Exactive Plus MS is upgradable to Q Exactive MS on site Software Features Operation Modes 3 Data System • Full-scan MS with high-resolution • High-performance PC with Intel® accurate-mass detection processor • All-ion fragmentation (AIF) in the HCD • High-resolution LCD color monitor collision cell with high-resolution accurate-mass detection (optional) • Microsoft Windows® 7 operating system • Source fragmentation of all ions in the • Microsoft Office® software package source region • Thermo Scientific™ Xcalibur™ processing • Positive/negative ion switching on and instrument control software chromatographic timescale • New workflow-based method editor • On-the-fly data-dependent AIF Data Processing Software • Optional extended mass range full-scan • Thermo Scientific™ TraceFinder™ MS and extended mass range AIF processing software for routine targeted and general unknown compound Exclusive Technologies screening, quantitation and indentification, • Optimum number of ions for all scans with compound database and spectra library • High-performance HCD collision cell for • Thermo Scientific™ MetQuest™ software, highest-performance AIF fragmentation automated processing software for (optional) quan/qual drug metabolic screening • Collision energy profiling using different and pharmacokinetic analyses collision energies for HCD fragmentation • Thermo Scientific™ MetWorks™ software, • Advanced signal processing metabolite identification processing • Interleaved operation software for the study of biotransformations • Thermo Scientific™ SIEVE™ software, differential analysis software for the study of metabolomics

• Thermo Scientific™ LCquan™ processing software for quantitative analysis of compounds supporting LC/MS data management activites within a 21 CFR Part 11 compliant environment • Mass Frontier™ software, chemically intelligent mass spectral interpretation software for metabolite identification and structural elucidation • Thermo Scientific™ Protein Deconvolution software for deconvolution of both isotopically resolved and unresolved spectra. Ideal for characterization of proteins, monoclonal antibodies, and similar molecules in their native (intact) states

Exactive Plus mass spectrometer with Thermo Scientific™ Dionex™ UltiMate™ 3000 Rapid Separation LC system

Product Specifications 420 mm 420   +41 61 716 77 00 +44 1442 233555 +65 6289 1190 910 mm +46 8 556 468 00 +886 2 8751 6655 +1 800 532 4752 Singapore Sweden Switzerland Taiwan UK/Ireland USA

+1 561 688 8700 +64 9 980 6700 +31 76 579 55 55 +46 8 556 468 00 940 mm +81 6 6885 1213 +82 2 3420 8600 Korea Latin America Netherlands New Zealand Norway Japan +45 70 23 62 60 +49 6103 408 1014 +358 9 3291 0200 +33 1 60 92 48 00

+91 22 6742 9494 +39 02 950 591 Denmark Finland France Germany India Italy

+32 53 73 42 41 +61 3 9757 4486 +1 800 530 8447  +400 650 5118 +43 810 282 206

+55 11 3731 5140 +1 800 810 5118

Exactive Plus (EMR) mass spectrometer without rotary vane forepump: Exactive Plus (EMR) mass spectrometer: (h × d × w) 940 × 830 mm × 910 (37 × 33× 36 inches) Operating environment must be 15–26 °C (59–78 °F) and relative humidity must be 40–70% with no condensation (385 kg 175 pounds) Forepump: pounds) 62 (136 kg System averages 2,500 (~9,000 W Btu/h) output when conditioning considering air needs 2 x 230 VAC ± 10% single phase, 15 2 x 230 single phase, 15 ± 10% VAC Ampere, 50/60 Hz, with earth ground for the instrument or 230120 single phase VAC with earth ground for the data system

PS30242_E 08/16S PS30242_E China Belgium Brazil Candada Australia Austria property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications,Not terms all products and pricing areare availablesubject to inchange. all countries. Please consult your local sales representative for details. © 2016 Thermo Fisher Scientific Inc. Windows, Microsoft, and Microsoft Office are Inteltrademarks is a trademark of Microsoft of Intel Corporation. Corporation. Mass Frontier is a trademark of HighChem Ltd.. All other trademarks are the www.thermofisher.com/dionex Not for use in diagnostic procedures. diagnostic in use for Not For Research Use Only. • • Weight • Dimensions • • Environment Environment 800 ± 30 kPa (8.0 ± 0.3 bar, 116 ± 4 psi)) 800 ± 30 kPa116 (8.0 ± 0.3 bar, High purity nitrogen gas supply (99% pure at Nitrogen • Gas • Power Installation Requirement