Bacteria Analytes
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Biofilm Formation by Moraxella Catarrhalis
BIOFILM FORMATION BY MORAXELLA CATARRHALIS APPROVED BY SUPERVISORY COMMITTEE Eric J. Hansen, Ph.D. ___________________________ Kevin S. McIver, Ph.D. ___________________________ Michael V. Norgard, Ph.D. ___________________________ Philip J. Thomas, Ph.D. ___________________________ Nicolai S.C. van Oers, Ph.D. ___________________________ BIOFILM FORMATION BY MORAXELLA CATARRHALIS by MELANIE MICHELLE PEARSON DISSERTATION Presented to the Faculty of the Graduate School of Biomedical Sciences The University of Texas Southwestern Medical Center at Dallas In Partial Fulfillment of the Requirements For the Degree of DOCTOR OF PHILOSOPHY The University of Texas Southwestern Medical Center at Dallas Dallas, Texas March, 2004 Copyright by Melanie Michelle Pearson 2004 All Rights Reserved Acknowledgements As with any grand endeavor, there was a large supporting cast who guided me through the completion of my Ph.D. First and foremost, I would like to thank my mentor, Dr. Eric Hansen, for granting me the independence to pursue my ideas while helping me shape my work into a coherent story. I have seen that the time involved in supervising a graduate student is tremendous, and I am grateful for his advice and support. The members of my graduate committee (Drs. Michael Norgard, Kevin McIver, Phil Thomas, and Nicolai van Oers) have likewise given me a considerable investment of time and intellect. Many of the faculty, postdocs, students and staff of the Microbiology department have added to my education and made my experience here positive. Many members of the Hansen laboratory contributed to my work. Dr. Eric Lafontaine gave me my first introduction to M. catarrhalis. I hope I have learned from his example of patience, good nature, and hard work. -
Yersiniosis** Non-Immediate Notification Epidemiology Program
YERSINIOSIS** NON-IMMEDIATE NOTIFICATION EPIDEMIOLOGY PROGRAM Event Name: YER Event Time Period: 1 year Clinical Description: N/A CDC Event Classification (N/A): N/A Massachusetts Event Classification (2015): Confirmed Positive culture of Yersinia enterocolitica or Yersinia pseudotuberculosis from throat swabs, mesenteric lymph nodes, peritoneal fluid, stool, and blood, OR Positive PCR result for Yersinia sp **If report is concerning a Yersinia pestis event, please refer to “Plague”. Y. pestis should not be entered as a YER** Test New or beyond Report Type Source Result Data Entry Type report period? Laboratory report Culture Clinical specimen Yersinia enterocolitica Yes New event OR CONFIRMED Yersinia No pseudotuberculosis Same event Select (no sub-type specified): Microorganism: PrId: Pt: xxx: Nom: Culture Select (sub-type specified): Microorganism: PrId: Pt: Islt: Nom: Bacterial subtyping Laboratory report Culture Stool Yersinia enterocolitica Yes New event OR CONFIRMED Yersinia No pseudotuberculosis Same event Select: Yersinia sp identified: Prld: Pt: Stl: Nom: Organism specific culture Laboratory report Culture Clinical specimen Yersinia enterocolitica Yes New event OR CONFIRMED Yersinia No pseudotuberculosis Same event Select: Yersinia sp identified: Prld : Pt : XXX : Nom : Organism specific culture Laboratory report PCR Clinical specimen Positive Yes New event CONFIRMED No Same event Select: Yersinia DNA XXX PCR MDPH Case Classification Manual Page 1 of 2 Yersiniosis Last modified: Jan 2017 Test New or beyond Report Type Source Result Data Entry Type report period? Laboratory report PCR Stool Detected Yes New event CONFIRMED No Same event Select: Y enterocol DNA: Stl: Ql: Non-probe: PCR Laboratory report PCR Stool Yersinia enterocolitica Yes New event CONFIRMED No Same event Select: GI path DNA+RNA: Pnl: Stl: Non-probe PCR We do not accept: Yersinia aldovae, Y. -
Blocking Transmission of Vector-Borne Diseases
International Journal for Parasitology: Drugs and Drug Resistance 7 (2017) 90e109 Contents lists available at ScienceDirect International Journal for Parasitology: Drugs and Drug Resistance journal homepage: www.elsevier.com/locate/ijpddr Review Blocking transmission of vector-borne diseases * Sandra Schorderet-Weber a, Sandra Noack b, Paul M. Selzer b, Ronald Kaminsky c, a Sablons 30, 2000 Neuchatel,^ Switzerland b Boehringer Ingelheim Animal Health GmbH, Binger Str. 173, 55216 Ingelheim, Germany c ParaC Consulting for Parasitology and Drug Discovery, Altenstein 13, 79685 Haeg-Ehrsberg, Germany article info abstract Article history: Vector-borne diseases are responsible for significant health problems in humans, as well as in companion Received 14 November 2016 and farm animals. Killing the vectors with ectoparasitic drugs before they have the opportunity to pass Accepted 22 January 2017 on their pathogens could be the ideal way to prevent vector borne diseases. Blocking of transmission Available online 30 January 2017 might work when transmission is delayed during blood meal, as often happens in ticks. The recently described systemic isoxazolines have been shown to successfully prevent disease transmission under Keywords: conditions of delayed pathogen transfer. However, if the pathogen is transmitted immediately at bite as it Vector-borne diseases is the case with most insects, blocking transmission becomes only possible if ectoparasiticides prevent Transmission blocking Drug discovery the vector from landing on or, at least, from biting the host. Chemical entities exhibiting repellent activity Speed of kill in addition to fast killing, like pyrethroids, could prevent pathogen transmission even in cases of im- mediate transfer. Successful blocking depends on effective action in the context of the extremely diverse life-cycles of vectors and vector-borne pathogens of medical and veterinary importance which are summarized in this review. -
E. Coli (Expec) Among E
Elucidating the Unknown Ecology of Bacterial Pathogens from Genomic Data Tristan Kishan Seecharran A thesis submitted in partial fulfilment of the requirements of Nottingham Trent University for the degree of Doctor of Philosophy June 2018 Copyright Statement I hereby declare that the work presented in this thesis is the result of original research carried out by the author, unless otherwise stated. No material contained herein has been submitted for any other degree, or at any other institution. This work is an intellectual property of the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the owner(s) of the Intellectual Property Rights. Tristan Kishan Seecharran i Acknowledgements I would like to express my sincere gratitude and thanks to my external advisor Alan McNally and director of studies Ben Dickins for their continued support, guidance and encouragement, and without whom, the completion of this thesis would not have been possible. Many thanks also go to the members of the Pathogen Research Group at Nottingham Trent University. I would like to thank Gina Manning and Jody Winter in particular for their invaluable advice and contributions during lab meetings. I would also like to thank our collaborators, Mikael Skurnik and colleagues from the University of Helsinki and Jukka Corander from the University of Oslo, for their much-appreciated support and assistance in this project and the published work on Yersinia pseudotuberculosis. -
ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization Of
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by KhartoumSpace ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization of Pasteurella multocida Vaccine Strains By: Hajir Badawi Mohammed Ahmed B.V.M Khartoum University (2006) Supervisor: Dr. Awad A. Ibrahim A dissertation submitted to the University of Khartoum in partial fulfillment of the requirements for the degree of M. Sc. in Microbiology Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum June, 2010 Dedication To my mother Father Brother, sister and friends With great love Acknowledgments First and foremost, I would like to thank my Merciful Allah, the most beneficent for giving me strength and health to accomplish this work. Then I would like to deeply thank my supervisor Dr. Awad A. Ibrahim for his advice, continuous encouragement and patience throughout the period of this work. My gratitude is also extended to prof. Mawia M. Mukhtar and for Dr. Manal Gamal El-dein, Institute of Endemic Disease. My thanks extend to members of Department of Microbiology Faculty of Veterinary Medicine for unlimited assistant and for staff of Central Laboratory Soba. I am grateful to my family for their continuous support and standing beside me all times. My thanks also extended to all whom I didn’t mention by name and to the forbearance of my friends, and colleagues who helped me. Finally I am indebted to all those who helped me so much to make this work a success. Abstract The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level. -
Supplementary Information
doi: 10.1038/nature06269 SUPPLEMENTARY INFORMATION METAGENOMIC AND FUNCTIONAL ANALYSIS OF HINDGUT MICROBIOTA OF A WOOD FEEDING HIGHER TERMITE TABLE OF CONTENTS MATERIALS AND METHODS 2 • Glycoside hydrolase catalytic domains and carbohydrate binding modules used in searches that are not represented by Pfam HMMs 5 SUPPLEMENTARY TABLES • Table S1. Non-parametric diversity estimators 8 • Table S2. Estimates of gross community structure based on sequence composition binning, and conserved single copy gene phylogenies 8 • Table S3. Summary of numbers glycosyl hydrolases (GHs) and carbon-binding modules (CBMs) discovered in the P3 luminal microbiota 9 • Table S4. Summary of glycosyl hydrolases, their binning information, and activity screening results 13 • Table S5. Comparison of abundance of glycosyl hydrolases in different single organism genomes and metagenome datasets 17 • Table S6. Comparison of abundance of glycosyl hydrolases in different single organism genomes (continued) 20 • Table S7. Phylogenetic characterization of the termite gut metagenome sequence dataset, based on compositional phylogenetic analysis 23 • Table S8. Counts of genes classified to COGs corresponding to different hydrogenase families 24 • Table S9. Fe-only hydrogenases (COG4624, large subunit, C-terminal domain) identified in the P3 luminal microbiota. 25 • Table S10. Gene clusters overrepresented in termite P3 luminal microbiota versus soil, ocean and human gut metagenome datasets. 29 • Table S11. Operational taxonomic unit (OTU) representatives of 16S rRNA sequences obtained from the P3 luminal fluid of Nasutitermes spp. 30 SUPPLEMENTARY FIGURES • Fig. S1. Phylogenetic identification of termite host species 38 • Fig. S2. Accumulation curves of 16S rRNA genes obtained from the P3 luminal microbiota 39 • Fig. S3. Phylogenetic diversity of P3 luminal microbiota within the phylum Spirocheates 40 • Fig. -
Review Article Yersinia Enterocolitica: Mode of Transmission, Molecular Insights of Virulence, and Pathogenesis of Infection
SAGE-Hindawi Access to Research Journal of Pathogens Volume 2011, Article ID 429069, 10 pages doi:10.4061/2011/429069 Review Article Yersinia enterocolitica: Mode of Transmission, Molecular Insights of Virulence, and Pathogenesis of Infection Yeasmin Sabina,1 Atiqur Rahman,2 Ramesh Chandra Ray,3 and Didier Montet4 1 Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka 1000, Bangladesh 2 Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh 3 Central Tuber Crops Research Institute, Bhubaneswar, India 4 Centre International de Recherche en Agronomie pour le Developpement (CIRAD), Montpellier, France Correspondence should be addressed to Yeasmin Sabina, y [email protected] Received 19 April 2011; Revised 28 May 2011; Accepted 5 June 2011 Academic Editor: Latiful Bari Copyright © 2011 Yeasmin Sabina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Although Yersinia enterocolitica is usually transmitted through contaminated food and untreated water, occasional transmission such as human-to-human, animal-to-human and blood transfusion associated transmission have also identified in human disease. Of the six Y. enterocolitica biotypes, the virulence of the pathogenic biotypes, namely, 1B and 2–5 is attributed to the presence of a highly conserved 70-kb virulence plasmid, termed pYV/pCD and certain chromosomal genes. Some biotype 1A strains, despite lacking virulence plasmid (pYV) and traditional chromosomal virulence genes, are isolated frequently from humans with gastrointestinal diseases similar to that produced by isolates belonging known pathogenic biotypes. Y. enterocolitica pathogenic biotypes have evolved two major properties: the ability to penetrate the intestinal wall, which is thought to be controlled by plasmid genes, and the production of heat-stable enterotoxin, which is controlled by chromosomal genes. -
Motiliproteus Sediminis Gen. Nov., Sp. Nov., Isolated from Coastal Sediment
Antonie van Leeuwenhoek (2014) 106:615–621 DOI 10.1007/s10482-014-0232-2 ORIGINAL PAPER Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment Zong-Jie Wang • Zhi-Hong Xie • Chao Wang • Zong-Jun Du • Guan-Jun Chen Received: 3 April 2014 / Accepted: 4 July 2014 / Published online: 20 July 2014 Ó Springer International Publishing Switzerland 2014 Abstract A novel Gram-stain-negative, rod-to- demonstrated that the novel isolate was 93.3 % similar spiral-shaped, oxidase- and catalase- positive and to the type strain of Neptunomonas antarctica, 93.2 % facultatively aerobic bacterium, designated HS6T, was to Neptunomonas japonicum and 93.1 % to Marino- isolated from marine sediment of Yellow Sea, China. bacterium rhizophilum, the closest cultivated rela- It can reduce nitrate to nitrite and grow well in marine tives. The polar lipid profile of the novel strain broth 2216 (MB, Hope Biol-Technology Co., Ltd) consisted of phosphatidylethanolamine, phosphatidyl- with an optimal temperature for growth of 30–33 °C glycerol and some other unknown lipids. Major (range 12–45 °C) and in the presence of 2–3 % (w/v) cellular fatty acids were summed feature 3 (C16:1 NaCl (range 0.5–7 %, w/v). The pH range for growth x7c/iso-C15:0 2-OH), C18:1 x7c and C16:0 and the main was pH 6.2–9.0, with an optimum at 6.5–7.0. Phylo- respiratory quinone was Q-8. The DNA G?C content genetic analysis based on 16S rRNA gene sequences of strain HS6T was 61.2 mol %. Based on the phylogenetic, physiological and biochemical charac- teristics, strain HS6T represents a novel genus and The GenBank accession number for the 16S rRNA gene T species and the name Motiliproteus sediminis gen. -
Escherichia Coli Saccharomyces Cerevisiae Bacillus Subtilis はB
研究開発等に係る遺伝子組換え生物等の第二種使用等に当たって執るべき拡散防止措 置等を定める省令の規定に基づき認定宿主ベクター系等を定める件 (平成十六年一月二十九日文部科学省告示第七号) 最終改正:令和三年二月十五日文部科学省告示第十三号 (認定宿主ベクター系) 第一条 研究開発等に係る遺伝子組換え生物等の第二種使用等に当たって執るべき拡散防止 措置等を定める省令(以下「省令」という。)第二条第十三号の文部科学大臣が定める認 定宿主ベクター系は、別表第一に掲げるとおりとする。 (実験分類の区分ごとの微生物等) 第二条 省令第三条の表第一号から第四号までの文部科学大臣が定める微生物等は、別表第 二の上欄に掲げる区分について、それぞれ同表の下欄に掲げるとおりとする。 (特定認定宿主ベクター系) 第三条 省令第五条第一号ロの文部科学大臣が定める特定認定宿主ベクター系は、別表第一 の2の項に掲げる認定宿主ベクター系とする。 (自立的な増殖力及び感染力を保持したウイルス及びウイロイド) 第四条 省令別表第一第一号ヘの文部科学大臣が定めるウイルス及びウイロイドは、別表第 三に掲げるとおりとする。 別表第1(第1条関係) 区 分 名 称 宿主及びベクターの組合せ 1 B1 (1) EK1 Escherichia coli K12株、B株、C株及びW株又は これら各株の誘導体を宿主とし、プラスミド又は バクテリオファージの核酸であって、接合等によ り宿主以外の細菌に伝達されないものをベクター とするもの(次項(1)のEK2に該当するものを除 く。) (2) SC1 Saccharomyces cerevisiae又はこれと交雑可能な 分類学上の種に属する酵母を宿主とし、これらの 宿主のプラスミド、ミニクロモソーム又はこれら の誘導体をベクターとするもの(次項(2)のSC2 に該当するものを除く。) (3) BS1 Bacillus subtilis Marburg168株、この誘導体又 はB. licheniformis全株のうち、アミノ酸若しく は核酸塩基に対する複数の栄養要求性突然変異を 有する株又は胞子を形成しない株を宿主とし、こ れらの宿主のプラスミド(接合による伝達性のな いものに限る。)又はバクテリオファージの核酸 をベクターとするもの(次項(3)のBS2に該当す るものを除く。) (4) Thermus属細菌 Thermus属細菌(T. thermophilus、T. aquaticus、 T. flavus、T. caldophilus及びT. ruberに限る。) を宿主とし、これらの宿主のプラスミド又はこの 誘導体をベクターとするもの (5) Rhizobium属細菌 Rhizobium属細菌(R. radiobacter(別名Agroba- cterium tumefaciens)及びR. rhizogenes(別名 Agrobacterium rhizogenes)に限る。)を宿主と し、これらの宿主のプラスミド又はRK2系のプラ スミドをベクターとするもの (6) Pseudomonas putida Pseudomonas putida KT2440株又はこの誘導体を 宿主とし、これら宿主への依存性が高く、宿主以 外の細胞に伝達されないものをベクターとするも の (7) Streptomyces属細菌 Streptomyces属細菌(S. avermitilis、S. coel- icolor [S. violaceoruberとして分類されるS. coelicolor A3(2)株を含む]、S. lividans、S. p- arvulus、S. griseus及びS. -
(12) United States Patent (10) Patent No.: US 9,018,158 B2 Onsoyen Et Al
US0090181.58B2 (12) United States Patent (10) Patent No.: US 9,018,158 B2 Onsoyen et al. (45) Date of Patent: Apr. 28, 2015 (54) ALGINATE OLIGOMERS FOR USE IN 7,208,141 B2 * 4/2007 Montgomery .................. 424/45 OVERCOMING MULTIDRUG RESISTANCE 22:49 R: R388 al al W . aSOC ea. N BACTERA 7,671,102 B2 3/2010 Gaserod et al. 7,674,837 B2 3, 2010 G d et al. (75) Inventors: Edvar Onsoyen, Sandvika (NO); Rolf 7,758,856 B2 T/2010 it. Myrvold, Sandvika (NO); Arne Dessen, 7,776,839 B2 8/2010 Del Buono et al. Sandvika (NO); David Thomas, Cardiff 2006.8 R 38 8. Melist al. (GB); Timothy Rutland Walsh, Cardiff 2003/0022863 A1 1/2003 Stahlang et al. (GB) 2003/0224070 Al 12/2003 Sweazy et al. 2004/OO73964 A1 4/2004 Ellington et al. (73) Assignee: Algipharma AS, Sandvika (NO) 2004/0224922 A1 1 1/2004 King 2010.0068290 A1 3/2010 Ziegler et al. (*) Notice: Subject to any disclaimer, the term of this 2010/0305062 A1* 12/2010 Onsoyen et al. ................ 514/54 patent is extended or adjusted under 35 U.S.C. 154(b) by 184 days. FOREIGN PATENT DOCUMENTS DE 268865 A1 1, 1987 (21) Appl. No.: 13/376,164 EP O324720 A1 T, 1989 EP O 506,326 A2 9, 1992 (22) PCT Filed: Jun. 3, 2010 EP O590746 A1 4f1994 EP 1234584 A1 8, 2002 (86). PCT No.: PCT/GB2O1 O/OO1097 EP 1714660 A1 10, 2006 EP 1745705 A1 1, 2007 S371 (c)(1), FR T576 M 3/1968 (2), (4) Date: Jan. -
Identification of Pasteurella Species and Morphologically Similar Organisms
UK Standards for Microbiology Investigations Identification of Pasteurella species and Morphologically Similar Organisms Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 1 of 28 © Crown copyright 2015 Identification of Pasteurella species and Morphologically Similar Organisms Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories UK Standards for Microbiology Investigations are produced in association with: Logos correct at time of publishing. Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 2 of 28 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Pasteurella species and Morphologically Similar Organisms Contents ACKNOWLEDGMENTS ......................................................................................................... -
Chapitre IV: Bacilles Gram Négatifs Aéro- Anaérobie Facultatifs ______Les Entérobactéries, Cours De Microbiologie Systématique Dr
Chapitre IV: Bacilles Gram négatifs aéro- anaérobie facultatifs __________________________________________Les entérobactéries, Cours de Microbiologie Systématique Dr. BOUSSENA 1. Enterobacteriaceae 1.1. Définition Les entérobactéries sont une famille très hétérogène pour ce qui est de leur pathogénie et de leur écologie. Les espèces qui composent cette famille sont en effet soit parasites (Shigella, Yersinia pestis), soit commensales (Escherichia coli, Proteus mirabilis, Klebsiella sp), soit encore saprophytes (Serratia sp, Enterobacter sp). 1.2. Habitat et pouvoir pathogène Le domaine des entérobactéries commensales ne se limite pas à l’intestin : on les trouve aussi dans la cavité buccale, au niveau des voies aériennes supérieures et sur les organes génitaux. Les entérobactéries sont présentes dans le monde entier et elles ont un habitat très large : eau douce, eau de mer (Alterococcus agarolyticus), sol, végétaux, animaux et elles peuvent contaminer des denrées alimentaires. Certaines espèces sont responsables de diarrhée et/ou d'infections opportunistes (infections urinaires, infections respiratoires, surinfections des plaies, septicémies, méningites...). 1.3. Répartition en genres Au sein des entérobactéries, on distingue de nombreux genres (Shigella, Escherichia, Enterobacter, Serratia, etc…) (tableau 3-1 et 3-2). La distinction entre les genres se fait par l'étude des caractères biochimiques dont les plus importants sont : fermentation du lactose, production d'indole, production d'uréase, production d'acetoïne (réaction dite VP+), utilisation du citrate, désamination du tryptophane. 1.4. Caractérisation des espèces Au sein de chaque genre, on individualise des espèces, par l'étude des caractères biochimiques ou antigéniques. Les entérobactéries possèdent toutes des antigènes de paroi (« somatiques ») ou antigènes O. Les entérobactéries mobiles possèdent en plus des antigènes de flagelle (« flagellaires ») ou antigènes H.