MRPL39 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science MRPL39 Rabbit pAb Catalog No.: A10023 Basic Information Background Catalog No. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in A10023 protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% Observed MW protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is 38kDa reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins Calculated MW comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical 38kDa/40kDa properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been Category described. A pseudogene corresponding to this gene is found on chromosome 5q. Primary antibody Applications WB, IHC Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:1000 - 1:4000 Gene ID Swiss Prot 54148 Q9NYK5 IHC 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 39-338 of human MRPL39 (NP_059142.2). Synonyms MRPL39;C21orf92;L39mt;MRP-L5;MRPL5;MSTP003;PRED22;PRED66;RPML5 Contact Product Information 400-999-6126 Source Isotype Purification Rabbit IgG Affinity purification [email protected] www.abclonal.com.cn Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using MRPL39 antibody (A10023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Ferret MRPL39 ORF Mammalian Expression Plasmid, N-Flag Tag
Ferret MRPL39 ORF mammalian expression plasmid, N-Flag tag Catalog Number: FG60159-NF General Information Plasmid Resuspension protocol Gene : mitochondrial ribosomal protein L39 1. Centrifuge at 5,000×g for 5 min. Official Symbol : MRPL39 2. Carefully open the tube and add 100 l of sterile water to Synonym : MRPL39 dissolve the DNA. Source : Ferret 3. Close the tube and incubate for 10 minutes at room cDNA Size: 1008bp temperature. RefSeq : XM_004757437.1 4. Briefly vortex the tube and then do a quick spin to Description concentrate the liquid at the bottom. Speed is less than Lot : Please refer to the label on the tube 5000×g. Vector : pCMV3-N-FLAG 5. Store the plasmid at -20 ℃. Shipping carrier : Each tube contains approximately 10 μg of lyophilized plasmid. The plasmid is ready for: Storage : • Restriction enzyme digestion The lyophilized plasmid can be stored at ambient temperature for three months. • PCR amplification Quality control : • E. coli transformation The plasmid is confirmed by full-length sequencing with primers • DNA sequencing in the sequencing primer list. Sequencing primer list : E.coli strains for transformation (recommended but not limited) pCMV3-F： 5’ CAGGTGTCCACTCCCAGGTCCAAG 3’ Most commercially available competent cells are appropriate for pcDNA3-R ： 5’ GGCAACTAGAAGGCACAGTCGAGG 3’ the plasmid, e.g. TOP10, DH5α and TOP10F´. Or Forward T7 ： 5’ TAATACGACTCACTATAGGG 3’ ReverseBGH ： 5’ TAGAAGGCACAGTCGAGG 3’ pCMV3-F and pcDNA3-R are designed by Sino Biological Inc. Customers can order the primer pair from any oligonucleotide supplier. Manufactured By Sino Biological Inc., FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. Fax :+86-10-51029969 Tel:+86- 400-890-9989 http://www.sinobiological.com Ferret MRPL39 ORF mammalian expression plasmid, N-Flag tag Catalog Number: FG60159-NF Vector Information All of the pCMV vectors are designed for high-level stable and transient expression in mammalian hosts.
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10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 MRPL39 Antibody (C-term) Blocking Peptide Synthetic peptide Catalog # BP16957b Specification MRPL39 Antibody (C-term) Blocking MRPL39 Antibody (C-term) Blocking Peptide - Peptide - Background Product Information Mammalian mitochondrial ribosomal proteins Primary Accession Q9NYK5 are encoded bynuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) MRPL39 Antibody (C-term) Blocking Peptide - Additional Information consist of asmall 28S subunit and a large 39S subunit. They have an estimated75% protein to rRNA composition compared to prokaryotic Gene ID 54148 ribosomes,where this ratio is reversed. Another difference between mammalianmitoribosomes Other Names and prokaryotic ribosomes is that the latter 39S ribosomal protein L39, mitochondrial, containa 5S rRNA. Among different species, the L39mt, MRP-L39, 39S ribosomal protein L5, proteins comprising themitoribosome differ mitochondrial, L5mt, MRP-L5, MRPL39, greatly in sequence, and sometimes C21orf92, MRPL5, RPML5 inbiochemical properties, which prevents easy Format recognition by sequencehomology. This gene Peptides are lyophilized in a solid powder encodes a 39S subunit protein. Two format. Peptides can be reconstituted in transcriptvariants encoding distinct isoforms solution using the appropriate buffer as have been described. Apseudogene needed. corresponding to this gene is found on chromosome 5q. Storage Maintain refrigerated at 2-8°C for up to 6 MRPL39 Antibody (C-term) Blocking months. For long term storage store at Peptide - References -20°C. Need, A.C., et al. Hum. Mol. Genet. Precautions 18(23):4650-4661(2009)Zhang, Z., et al. This product is for research use only. Not Genomics 81(5):468-480(2003)O'Brien, T.W.
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Supplementary table 1: Proteins identified by proteomics Protein Accession Gene Symbol Ratio Protein Name mitogen-activated protein kinase-activated protein kinase 2 gi|32481209 MAPKAPK2 0.04 isoform 2 gi114155133 DNAH9 0.05 dynein, axonemal, heavy chain 9 isoform 2 gi|4506145 PRSS1 0.09 protease, serine, 1 preproprotein gi|4507823 UGT2B11 0.10 UDP glucuronosyltransferase 2 family, polypeptide B11 gi|31377593 RANBP3L 0.10 RAN binding protein 3-like gi|45827809 MAP3K6 0.10 mitogen-activated protein kinase kinase kinase 6 gi|31795544 ORC1L 0.19 origin recognition complex, subunit 1 gi|4504191 MSH6 0.23 mutS homolog 6 gi|4503971 GDI1 0.26 GDP dissociation inhibitor 1 gi|4502197 TRIM23 0.27 ADP-ribosylation factor domain protein 1 isoform alpha gi|30410794 PSME3 0.28 proteasome activator subunit 3 isoform 1 gi|4506703 RPS24 0.29 ribosomal protein S24 isoform c gi|8923415 MARCH5 0.30 membrane-associated ring finger (C3HC4) 5 gi|39725634 LARP1 0.30 la related protein isoform 1 RBM14/RBM4 NP_002887 fusion 0.31 Transcriptional coactivator CoAZ gi|4506891 SET 0.33 SET translocation (myeloid leukemia-associated) gi|57863257 TCP1 0.33 T-complex protein 1 isoform a gi|25777612 PSMD3 0.35 proteasome 26S non-ATPase subunit 3 gi|22748937 XPO5 0.35 exportin 5 gi68303565 PSMA8 0.36 proteasome alpha 8 subunit isoform 3 gi|29789090 RCC2 0.37 regulator of chromosome condensation 2 gi113412226 LOC642098 0.38 similar to ribosomal protein L31 gi|4506189 PSMA7 0.38 proteasome alpha 7 subunit gi|38201619 EIF4G1 0.38 eukaryotic translation initiation factor 4 gamma,
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Table 1 the Statistical Metrics for Key Differentially Expressed Genes (Degs)
Table 1 The statistical metrics for key differentially expressed genes (DEGs) Illumina Id Gene Symbol logFC p Value FDR t value Regulation Gene name ILMN_1757497 VGF 4.848711 1.27E-13 6.11E-09 48.36804 UP VGF nerve growth factor inducible ILMN_2193706 HRK 3.058491 2.89E-10 3.47E-06 22.91096 UP harakiri, BCL2 interacting protein ILMN_2182198 MRPL58 1.8013 5.14E-10 3.64E-06 21.65441 UP mitochondrial ribosomal protein L58 ILMN_1699772 RRAGD 2.331637 6.56E-10 3.64E-06 21.14388 UP Ras related GTP binding D ILMN_1695969 TMPRSS15 2.158671 6.81E-10 3.64E-06 21.06686 UP transmembrane protease, serine 15 ILMN_1754002 IL36B 4.161387 3.21E-09 1.10E-05 18.09187 UP interleukin 36, beta ILMN_1656134 CNOT7 1.387716 4.97E-09 1.49E-05 17.32692 UP CCR4-NOT transcription complex subunit 7 ILMN_1658499 SYT13 1.653015 6.80E-09 1.59E-05 16.79842 UP synaptotagmin 13 ILMN_1684836 AKAP12 1.291595 8.20E-09 1.68E-05 16.48925 UP A-kinase anchoring protein 12 ILMN_1799519 IL36B 4.299495 9.58E-09 1.68E-05 16.23691 UP interleukin 36, beta ILMN_1678757 BCYRN1 3.056229 9.58E-09 1.68E-05 16.23661 UP brain cytoplasmic RNA 1 ILMN_1726743 MRPS30 1.302467 1.08E-08 1.73E-05 16.04765 UP mitochondrial ribosomal protein S30 ILMN_1777976 SLC25A26 1.609861 1.37E-08 2.06E-05 15.66752 UP solute carrier family 25 member 26 mitochondrially encoded ILMN_3308961 MT-ND6 3.019664 1.61E-08 2.15E-05 15.41788 UP NADH:ubiquinoneoxidoreductase core subunit 6 ILMN_2380999 RECQL 1.437639 2.36E-08 2.72E-05 14.83968 UP RecQ like helicase ILMN_3233229 SNHG7 1.202524 2.50E-08 2.72E-05 14.7569 UP small
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Open Min-Joon-Upload-Final.Pdf
The Pennsylvania State University The Graduate School Eberly College of Science MAMMALIAN MITOCHONDRIAL RIBOSOMAL PROTEINS : A MATTER OF LIFE AND DEATH A Dissertation in Biochemistry, Microbiology and Molecular Biology by Min-Joon Han © 2010 Min-Joon Han Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August 2010 The dissertation of Min-Joon Han was reviewed and approved* by the following: Emine C. Koc Assistant Professor of Biochemistry and Molecular Biology Dissertation Co-Advisor Co-Chair of Committee Hasan Koc Research Assistant Professor of Biochemistry and Molecular Biology Dissertation Co-Advisor Co-Chair of Committee Craig E. Cameron Paul Berg Professor of Biochemistry and Molecular Biology Wendy Hanna-Rose Associate Professor of Biochemistry and Molecular Biology Teh-hui Kao Professor of Biochemistry and Molecular Biology Seogchan Kang Professor of Plant pathology Scott B. Selleck Professor of Biochemistry and Molecular Biology Head of the Department of Biochemistry and Molecular Biology *Signatures are on file in the Graduate School ii ABSTRACT Human mitochondria are essential for cell survival while playing key roles in programmed cell death also called apoptosis. First of all, mitochondria are the main source of energy for the eukaryotic cell. Mitochondria produce more than 90% of the energy used by mammalian cells in a process referred to as oxidative phosphorylation. This demanding mechanism requires a lot of proteins, which are nucleus-encoded, synthesized in cytoplasm, and imported into mitochondria. However, it also requires 13 mitochondrial-encoded proteins. Mitochondria have their own 16.5 kb circular genome (mtDNA) and ribosome to translate these 13 proteins.
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