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Using AFLP Markers in Persian Gulf

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Using AFLP Markers in Persian Gulf Genetic diversity of Bartail flathead (Platycephalus indicus (Linnaeus,1758)) using AFLP markers in Persian Gulf Item Type thesis Authors Fekrandish, Hakimeh Publisher Islamic Azad University, Science and Research Branch, Tehran, Fisheries Download date 30/09/2021 16:10:32 Link to Item http://hdl.handle.net/1834/34381 ‌ ‌ ‌ ‌ ‌ ‌ أ ‌ ‌ ‌ ب ‌ داًشگاُ آساداسﻻهی ٍاحدعلَهَتحقیقات تعهدنامهاصالترسالهیاپایاننامه ایٌدبًتحکیوِ فکزاًدیشداًـأهَختْوقطؼذکتشیتخصصیذسسؿتِشیﻻتکْذستبسیخ3/ 9/ 1392اصپبیبًٌبهِ / " سػبلْخَدتحتؼٌَاى هطالعِ تٌَع صًتیکی هاّی سهیي کي دم ًَاری "AFLP Platycephalusindicus(Linnaeus,1758 )) ( در خلیج فارس با استفادُ اس ًشاًگزّای ثبکؼجٌوشُ .......ٍدسخِ .............دفبػٌوَدّبهجذیػٌَیلْوتؼْذهیـَم: 1(ایٌپبیبًٌبهِ / سػبلْحبصلتحقیقَپظٍّـبًدبهـذّتػَطبیٌدبًججَدَّدسهَاسدیکْبصدػتبٍسدّبیؼلویَپظٍّـیذیگشاى)اػوبصپبیبً ًبهِ،کتبة،هقبلَْ ... (اػتفبدٌّوَدّبم،هطبثقضَاثطَسٍیْوَخَد،ًبهوٌجؼوَسداػتفبدػَّبیشهـخصبتأًشادسفْشػتوشثَطْزکشٍدسخک سدّبم. 2)ایٌپبیبًٌبهِ / سػبلْقجﻻ " ثشایذسیبفتْیچوذسکتحصیلی)ّوؼطح،پبئیٌتشیبثبﻻتش(دسػبیشداًـگبّْبٍهؤؼػبتأهَصؼؿبلیبسائٌْـذّبػت. 3(چٌبًچْجؼذاصفشاغتبصتحصیل،قصذاػتفذَّّشگًَْجْشّجشداسیبػوبصچبپکتبة،ثجتبختشاػَ ... اصایٌپبیبًٌبهْذاؿتْجبؿن،اصحَصّوؼبًٍتپظٍّـیَاحذهدَصّبیوشثَطْشااخزًوبین. 4(چٌبًچْذسّشهقطؼضهبًیخﻻفوَاسدفَقثبثتـَد،ػَاقجٌبؿیبصآًشاهیپزیشهٍَاحذداًـگبّیودبصاػتجبایٌدبًجوطبثقض ٍاثطَهقشساتشفتبسًوَدَّدسصَستبثطبلوذسکتحصیلیبهْیچگًَْبدػبییٌخَاّوذاؿت. ًبهًَبهخبًَادگی:حکیوِ فکشاًذیؾ تبسیخَاهضبء: ت ‌ دانشگاه آزاد اسﻻمی واحد علوم و تحقیقات دانشکده کشاورزی و منابع طبیعی ،گروه شیﻻت Ph.D رساله دکتری رشته شیﻻت ) ( عنوان: هطالعه تنوع شنتیکی هاهی زهین کن دم نواری ))Platycephalusindicus(Linnaeus,1758( در خلیج فارس با استفاده از نشانگرهای AFLP استادان راهنما: دکتر ابوالقاسن کوالی دکتر سید جواد حسینی استاد مشاور: دکتر ههدی سلطانی نگارش: حکیوه فکراندیش پاییس 2931 ث ISLAMIC AZAD UNIVERSITY SCIENCE AND RESEARCH BRANCH Faculty of Agriculture and Natural Resourse Department of Fisheries Ph.D Thesis Fisheries Subject: Genetic diversity of Bartail flathead (Platycephalusindicus(Linnaeus,1758)) using AFLP markers in Persian Gulf Thesis Advisors: A. Kamali Ph.D. S. J. Hosseini Ph.D. Consulting Advisor: M SoltaniPh.D. By: Hakimeh Fekrandish Autumn 2013 ج سپاسگشاری پیؾ اص ّش چیض خذاًٍذ ثضسگ سا ؿبکشم کِ تَفیق داد تب ؿبیذ ثب کؼت ػلن ٍ داؾً ثتَاًن رسُ ای اص حق ثٌذگی اؽ سا ثدبی آٍسم. خذاًٍذ هْشثبًی کِ دس توبهی لحظبت حبضش ٍ ًبظش ثش احَال هي ثَدُ ٍ دس کلیِ هشاحل صًذگی هشا دس پٌبُ خَد ًگِ داؿتِ ٍ ّوَاسُ دس سػیذى ثِ اّذافن ثِ هي کوک کشدُ اػت. اص اػبتیذ اسخوٌذم خٌبة آقبی دکتش ػیذ خَاد حؼیٌیؼضَ ّیبت ػلوی هشکض هطبلؼبت داًـگبُ خلیح فبسع ثؿَْش ٍدکتش اثَالقبػن کوبلی کِ ػلیشغن هـغلِ کبسی فشاٍاى ساٌّوبیی ایي سػبلِ سا ثش ػْذُ داؿتِ ٍدس توبهی هشاحل اًدبم ایي پشٍطُ اص حوبیت ّبی ثی دسیغ ٍ کوک ّب ٍ ساٌّوبیی ّبی اسصًذُ ؿبى ثشخَس داس ثَدُ ام ثی ًْبیت ػپبػگضاسم ٍ ؼػبدت ٍ ػﻻهت سٍص افضٍى ایـبى سا اص دسگبُ خذاًٍذ هتؼبل خَاػتبسم. اص اػتبد ثضسگَاسم خٌبة آقبی دکتش هْذی ػلطبًی کِ ثِ ػٌَاى اػتبد هـبٍس دس طَل اًدبم ایي پشٍطُ ّوَاسُ اص هؼبػذت ّب ٍ کوک ّبی ثی دسیغـبى ثْشُ هٌذ ثَدُ ام صویوبًِ ػپبػگضاسی هی کٌن. اص سیبػت هحتشم هشکض هطبلؼبت ٍ پظؾٍّ ّبی داًـگبُ خلیح فبسع ثؿَْش خٌبة آقبی دکتش هحوذ هذسػی ثِ خْت کلیِ کوک ّب ٍ ّوکبسی ّبی ﻻصم ػپبػگضاسم. اص توبهی ػضیضاى هشکض هطبلؼبت ٍ پظؾٍّ ّبی داًـگبُ خلیح فبسع ثؿَْش کِ ّش یک ثِ ًحَی دس اخشای ایي پشٍطُ خبلصبًِ ایٌدبًت سا یبسی ًوَدًذ خصَصب پشػٌل هحتشم ثخؾ طًتیک آقبی هٌْذع فقیِ احوذاًی، آقبی هٌْذع کبهیبة، آقبی هٌْذع فخشی، آقبی دکتش قبػوی ٍ ثقیِ دػٍتبى ػشکبس خبًن هٌْذػأٍیظگبى، خبًن هٌْذع چشاغی ٍ خبًن هٌْذع ًبهدَ ٍ ثقیِ ثضسگَاساى ػپبػگضاسم. دس ًْبیت اص اػتبد ػضیضم خٌبة آقبی دکتش حبهذ هػََی ثبثتجِ خبطشساٌّوبیی ّبی ثؼیبس اسصًذُ دس طی اًدبم ایي پشٍطُ صویوبًِ ػپبػگضاسم. ثی ؿک ثذٍى ّوکبسی ایـبى اًدبم ایي پشٍطُ ثشاین ًبهوکي ثَد. ‌ ‌ ‌ ‌ ‌ ح ‌ ‌ تقدیم هب هادر هْزباًن کِ گزهای عشق ٍ هحبتش را در تواهی لحظات سًدگی ام احساس کزدُ ام پدر ارجوٌدم کِ اس ّیچ تﻻشی در جْت هَفقیت فزسًداًش فزٍگذار ًکزدُ است، ٍ بزادر عشیشم احود کِ دٍستی هْزباى ٍ تکیِ گاّی هحکن است. ‌ ‌ ‌ ‌ خ ‌ ‌ ‌ ‌ فْزست هطالب عٌَاى ....................................................................................................................................... صفحِ چکیذُ .......................................................................................................................................................... 1 فصل اٍل: کلیات تحقیق 1-1- هقذهِ ................................................................................................................................................. 4 1-2- کلیبت ................................................................................................................................................. 8 1-2-1- خلیح فبسع ................................................................................................................................... 8 1-2-1-1- هَقؼیت خغشافیبیی خلیح فبسع ............................................................................................... 8 1-2-1-2- ٍیظگی اکَلَطیکی خلیح فبسع ................................................................................................ 8 ػ-3-1-2-1وقػٌدیَ سیختؿٌبػی دس خلیح فبسع ............................................................................. 9 1-2-2- خبًَادُ صهیيکي هبّیبى Platycephalidaeیب هبّیبى ػشهؼطحFlatheads .......................... 9 1-2-2-1- هـخصبت ثبسص صهیي کي هبّیبى ............................................................................................ 10 1-2-2-2- هؼشفی گًَِ صهیيکي دمًَاسی)(Platycephalus indicus(Linnaeus,1758( ............ 10 1-2-2-3- پشاکؾٌ، صیؼتگبُ ٍ خصَصیبت هَسفَلَطیک هبّی صهیيکي دمًَاسی ................................. 11 1-2-2-4- خصَصیبتتَلیذ هثلی ٍ تغزیِ هبّی صهیيکي دمًَاسی ...................................................... 14 1-2-2-5-آهبس صیذ هبّی صهیيکي دمًَاسی)(Platycephalus indicus(Linnaeus,1758( دس ایشاى 16........................................................................................................................................................... د 1-2-3- هَقؼیت هٌبطق هَسد هطبلؼِ ........................................................................................................ 17 1-2-3-1- خَسهػَی ...............................................................................................................................17 1-2-3-2- ثحشکبى ................................................................................................................................... 18 ؿ -3-3-2-1یف ...................................................................................................................................... 18 1-2-3-4- هطبف ..................................................................................................................................... 18 1-2-3-5- چبسک ..................................................................................................................................... 19 1-2-3-6- ثٌذسػجبع ............................................................................................................................... 19 1-2-4 - اًَاع ًـبًگشّب ........................................................................................................................... 20 1-2-4-1- ًـبًگشّبی هَسفَلَطیکی ....................................................................................................... 20 1-2-4-2- ًـبًگشّبی ػیتَطًتیکی .........................................................................................................20 1-2-4-3- ًـبًگشّبی هَلکَلی ............................................................................................................... 21 1-2-4-3-1-ًـبًگشّبی پشٍتئیٌی ......................................................................................................... 22 1-2-4-3-2- ًـبًگشّبیDNA ............................................................................................................ 23 1-2-4-3-2-1-ًـبًگشّبیmtDNA ............................................................................................... 24 24 ......................................................................................................................... RFLP-2-2-3-4-2-1 25 ........................................................................................................................ RAPD-3-2-3-4-2-1 25 ............................................................................................................................. SNP-4-2-3-4-2-1 26 .............................................................................................................................. EST-5-2-3-4-2-1 1-2-4-3-2-6-سیض هبَّاسّْب ............................................................................................................... 26 27 ......................................................................................................................... AFLP-7-2-3-4-2-1 1-2-5- ًـبًگش Amplified Fragment length polymorphism(AFLP(............................... 27 1-2-5-1-هضایبی AFLP ........................................................................................................................ 29 1-2-5-2-هؼبیتAFLP ......................................................................................................................... 29 1-2-5-3-کبسثشد ًـبًگشAFLP ............................................................................................................. 30 ذ 1-2-6- فشضیِ ّبی تحقیق ...................................................................................................................... 31 1-2-7- اّذاف تحقیق .............................................................................................................................. 31 فصل دٍم: هشٍسیجشادثیبتتحقیقَپیـیٌْتحقیق
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