For Research Use Only CD79A

Polyclonal www.ptglab.com Catalog Number:22349-1-AP

Basic Information Catalog Number: GenBank Accession Number: Recommended Dilutions: 22349-1-AP BC113733 WB 1:500-1:2000 Size: GeneID (NCBI): IHC 1:20-1:200 45 μg/150 μl 973 Source: Full Name: Rabbit CD79a molecule, immunoglobulin-associated Isotype: alpha IgG Calculated MW: Purification Method: 226aa,25 kDa affinity purification Observed MW: Immunogen Catalog Number: 38-42 kDa AG17924

Applications Tested Applications: Positive Controls: IHC, WB, ELISA WB : Daudi cells; Raji cells, Ramos cells Species Specificity: IHC : human tonsillitis tissue; human Note: suggested angen retrieval with TE buffer pH 9.0; (*) Alternavely, angen retrieval may be performed with citrate buffer pH 6.0

CD79A, also named as B-cell antigen complex-associated alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which Background Information contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

Notable Publications Author Pubmed ID Journal Application

Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this This product is exclusively available under product please contact: Proteintech Group brand and is not available to T: 1 (888) 4PTGLAB (1-888-478-4522) (toll E: [email protected] purchase from any other manufacturer. free in USA), or 1(312) 455-8498 (outside W: ptglab.com USA) Selected Validation Data

Various lysates were subjected to SDS PAGE of paraffin-embedded Immunohistochemistry of paraffin-embedded followed by western blot with 22349-1-AP (CD79A human tonsillitis tissue slide using 22349-1-AP( human tonsillitis tissue slide using 22349-1-AP( antibody) at dilution of 1:1000 incubated at room CD79A Antibody) at dilution of 1:200 (under 10x CD79A Antibody) at dilution of 1:200 (under 40x temperature for 1.5 hours lens) lens)