CD79A Polyclonal ANTIBODY

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For Research Use Only CD79A Polyclonal ANTIBODY www.ptglab.com Catalog Number:22349-1-AP Basic Information Catalog Number: GenBank Accession Number: Recommended Dilutions: 22349-1-AP BC113733 WB 1:500-1:2000 Size: GeneID (NCBI): IHC 1:20-1:200 45 μg/150 μl 973 Source: Full Name: Rabbit CD79a molecule, immunoglobulin-associated Isotype: alpha IgG Calculated MW: Purification Method: 226aa,25 kDa Antigen affinity purification Observed MW: Immunogen Catalog Number: 38-42 kDa AG17924 Applications Tested Applications: Positive Controls: IHC, WB, ELISA WB : Daudi cells; Raji cells, Ramos cells Species Specificity: IHC : human tonsillitis tissue; human Note: suggested angen retrieval with TE buﬀer pH 9.0; (*) Alternavely, angen retrieval may be performed with citrate buﬀer pH 6.0 CD79A, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which Background Information contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Notable Publications Author Pubmed ID Journal Application Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this This product is exclusively available under product please contact: Proteintech Group brand and is not available to T: 1 (888) 4PTGLAB (1-888-478-4522) (toll E: [email protected] purchase from any other manufacturer. free in USA), or 1(312) 455-8498 (outside W: ptglab.com USA) Selected Validation Data Various lysates were subjected to SDS PAGE Immunohistochemistry of paraffin-embedded Immunohistochemistry of paraffin-embedded followed by western blot with 22349-1-AP (CD79A human tonsillitis tissue slide using 22349-1-AP( human tonsillitis tissue slide using 22349-1-AP( antibody) at dilution of 1:1000 incubated at room CD79A Antibody) at dilution of 1:200 (under 10x CD79A Antibody) at dilution of 1:200 (under 40x temperature for 1.5 hours lens) lens).

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PAX5 Expression in Acute Leukemias: Higher B-Lineage Specificity Than Cd79a and Selective Association with T(8;21)-Acute Myelogenous Leukemia
[CANCER RESEARCH 64, 7399–7404, October 15, 2004] PAX5 Expression in Acute Leukemias: Higher B-Lineage Specificity Than CD79a and Selective Association with t(8;21)-Acute Myelogenous Leukemia Enrico Tiacci,1 Stefano Pileri,2 Annette Orleth,1 Roberta Pacini,1 Alessia Tabarrini,1 Federica Frenguelli,1 Arcangelo Liso,3 Daniela Diverio,4 Francesco Lo-Coco,5 and Brunangelo Falini1 1Institutes of Hematology and Internal Medicine, University of Perugia, Perugia, Italy; 2Unit of Hematopathology, University of Bologne, Bologne, Italy; 3Section of Hematology, University of Foggia, Foggia, Italy; 4Department of Cellular Biotechnologies and Hematology, University La Sapienza of Rome, Rome, Italy; and 5Department of Biopathology, University Tor Vergata of Rome, Rome, Italy ABSTRACT (13, 16). PAX5 expression also occurs in the adult testis and in the mesencephalon and spinal cord during embryogenesis (17), suggesting an The transcription factor PAX5 plays a key role in the commitment of important role in the development of these tissues. hematopoietic precursors to the B-cell lineage, but its expression in acute Rearrangement of the PAX5 gene through reciprocal chromosomal leukemias has not been thoroughly investigated. Hereby, we analyzed routine biopsies from 360 acute leukemias of lymphoid (ALLs) and mye- translocations has been described in different types of B-cell malig- loid (AMLs) origin with a specific anti-PAX5 monoclonal antibody. Blasts nancies (18–23), and, more recently, PAX5 has also been shown to be from 150 B-cell ALLs showed strong PAX5 nuclear expression, paralleling targeted by aberrant hypermutation in Ͼ50% of diffuse large B-cell that of CD79a in the cytoplasm. Conversely, PAX5 was not detected in 50 lymphomas (24).
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Human and Mouse CD Marker Handbook Human and Mouse CD Marker Key Markers - Human Key Markers - Mouse
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CD Markers Are Routinely Used for the Immunophenotyping of Cells
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Turk J Hematol 2006; 23:173-181 REVIEW ARTICLE © Turkish Society of Hematology Recent advances in diagnosis, prognosis and biology of small B cell lymphomas Gülşah Kaygusuz, Işınsu Kuzu Department of Pathology, Ankara University, Faculty of Medicine, Ankara, Turkey [email protected] INTRODUCTION eral blood, bone marrow, and/or lymph nodes. Small B cell lymphomas are mature B cell lym- The term SLL is used for non-leukemic cases phoid neoplasms arising from the various differ- having the morphology and immunophenotype entiation stages of B cell development. Although of CLL. Many cases of CLL/SLL are thought to in the World Health Organization (WHO) classi- correspond to the recirculating naïve B cells. fication of lymphomas, they are described based Cases that show Ig gene variable region muta- on their morphology, phenotype and genetics, the tions may correspond to a subset of memory B differential diagnosis can be difficult because of cells [1]. some overlapping characteristics. Differences ob- served in the clinical outcome of cases represent- The nodal presentation of CLL/SLL is char- ing the same entity have also made it difficult to acterized by the diffuse infiltration of small lym- understand fully the biology of the small B cell phocytes admixed with prolymphocytes and lymphomas, but interpretation of the results of para-immunoblasts, giving rise to proliferation molecular profiling studies has helped patholo- centers or pseudofollicles. CLL/SLL cells are gists and clinicians in this regard. Some specific small lymphocytes with round nucleus, clumped genetic changes defined on the neoplastic B cells chromatin and scant cytoplasm. Nucleoli are have revealed the route of lymphomagenesis.
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Atpase, Na+/K+ Transporting, Alpha 3 Polypeptide Homologous to 3'UTR
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REVIEWS Genetic Defects in B-Cell Development and Their Clinical Consequences H Abolhassani,1,2 N Parvaneh,1 N Rezaei,1 L Hammarström,2 A Aghamohammadi1 1Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran 2Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden n Abstract Expression of selected genes in hematopoietic stem cells has been identified as a regulator of differentiation of B cells in the liver and bone marrow. Moreover, naïve B cells expressing surface immunoglobulin need other types of genes for antigen-dependent development in secondary lymphoid organs. Many advanced molecular mechanisms underlying primary antibody deficiencies in humans have been described. We provide an overview of the mutations in genes known to be involved in B-cell development and their clinical consequences. Key words: Genetic disorder. B-cell development. Primary antibody deficiencies. Clinical phenotypes. n Resumen Se ha identificado la expresión de genes seleccionados en las células pluripotenciales de médula ósea como reguladores de la diferenciación de las células B en el hígado y en médula ósea. Sin embargo, las células B naïve que expresan inmunoglubulinas de superficie, necesitan otros tipos de genes para su desarrollo en los órganos linfoides secundarios dependienteS de antígeno. Se han descrito muchos mecanismos moleculares avanzados que subrayan las inmunodeficiencias en humanos y esta revisión constituye una visión general de la mutación en todos los genes conocidos involucrados en el desarrollo de las células B y sus consecuencias clínicas. Palabras clave: Alteraciones genéticas. Desarrollo de las células B.
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Hindawi Journal of Immunology Research Volume 2017, Article ID 4807853, 17 pages https://doi.org/10.1155/2017/4807853 Review Article Interleukin-7 and Immunosenescence Vanloan Nguyen, Andrew Mendelsohn, and James W. Larrick Panorama Research Institute, 1230 Bordeaux Drive, Sunnyvale, CA 94089, USA Correspondence should be addressed to Vanloan Nguyen; [email protected] Received 6 December 2016; Revised 1 February 2017; Accepted 19 February 2017; Published 6 April 2017 Academic Editor: Alessandra Santos Copyright © 2017 Vanloan Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The age of an individual is an important, independent risk factor for many of the most common diseases afflicting modern societies. Interleukin-7 (IL-7) plays a central, critical role in the homeostasis of the immune system. Recent studies support a critical role for IL-7 in the maintenance of a vigorous healthspan. We describe the role of IL-7 and its receptor in immunosenescence, the aging of the immune system. An understanding of the role that IL-7 plays in aging may permit parsimonious preventative or therapeutic solutions for diverse conditions. Perhaps IL-7 might be used to “tune” the immune system to optimize human healthspan and longevity. 1. Introduction To understand the IL-7 network, we begin with a descrip- tion of IL-7, the IL-7 receptor, and downstream signal trans- One’s chronological age is an important, independent risk duction. We document how aging affects various parts of factor for many of the most common diseases afflicting the the immune system, B cells, T cells, and so forth, in an effort aging population of the world.
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Burkitt Lymphoma
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LECTURE 12 B Lymphocytes Surface Receptors and Activation
LECTURE: 12 Title: B-LYMPHOCYTES SURFACE RECEPTORS AND THEIR ACTIVATION LEARNING OBJECTIVES: The student should be able to: • Identify the percentage of B-lymphocytes in comparison to other circulating lymphoid cells. • Distinguish B-lymphocytes in regarding to their cell surface receptors. • Identify the definitive B-cell surface markers. • Determine to which type of immune receptor families this B-cell definitive marker belongs? • Identify the surface immunoglobulin isotypes (antigen-receptors). • Describe the B-cell receptor complex (BCR). • Enumerate some B-cell markers, and indicate their importance in immunity. • Explain the reason that, CD5+ B-lymphocytes are considered as a distinctive Cell subset. • Indicate which B-cell marker (s), enhance B-cell phagocytosis. • Enumerate some of the B-cell receptors which are important in B-cell- cooperations. • Explain the serological technique used to visualize the immunoglobulins in the cytoplasm of the plasma cell. • Describe the mechanism of signal transduction is performed by BCRs. LECTURE REFRENCE: 1. TEXTBOOK: ROITT, BROSTOFF, MALE IMMUNOLOGY. 6th edition. Chapter 2. pp. 29-30 2. TEXTBOOK: ABUL K. ABBAS. ANDREW H. LICHTMAN. CELLULAR AND MOLECULAR IMMUNOLOGY. 5TH EDITION. Chapter. 7 .pg 129-151. 3. TEXTBOOK: MALE, COOKE. OWEN, TROWSDALE, CHAMPION ADVANCE IMMUNOLOGY. Chapter 3. pp. 3.7. Chapter 6. pp 6.7. 1 B-LYMPHOCYTE SURFACE RECEPTORS AND B CELL ACTIVATRION INTRODUCTION B cells are characterized by their surface immunoglobulins. They represent about 5-15 % of the circulating lymphoid pool, and are classically defined by the presence of surface immunoglobulins. These immunoglobulin markers are made by the B cells themselves, and are inserted into the surface membrane where they act as specific antigen receptors.
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Human CD Marker Chart Reviewed by HLDA1 Bdbiosciences.Com/Cdmarkers
BD Biosciences Human CD Marker Chart Reviewed by HLDA1 bdbiosciences.com/cdmarkers 23-12399-01 CD Alternative Name Ligands & Associated Molecules T Cell B Cell Dendritic Cell NK Cell Stem Cell/Precursor Macrophage/Monocyte Granulocyte Platelet Erythrocyte Endothelial Cell Epithelial Cell CD Alternative Name Ligands & Associated Molecules T Cell B Cell Dendritic Cell NK Cell Stem Cell/Precursor Macrophage/Monocyte Granulocyte Platelet Erythrocyte Endothelial Cell Epithelial Cell CD Alternative Name Ligands & Associated Molecules T Cell B Cell Dendritic Cell NK Cell Stem Cell/Precursor Macrophage/Monocyte Granulocyte Platelet Erythrocyte Endothelial Cell Epithelial Cell CD1a R4, T6, Leu6, HTA1 b-2-Microglobulin, CD74 + + + – + – – – CD93 C1QR1,C1qRP, MXRA4, C1qR(P), Dj737e23.1, GR11 – – – – – + + – – + – CD220 Insulin receptor (INSR), IR Insulin, IGF-2 + + + + + + + + + Insulin-like growth factor 1 receptor (IGF1R), IGF-1R, type I IGF receptor (IGF-IR), CD1b R1, T6m Leu6 b-2-Microglobulin + + + – + – – – CD94 KLRD1, Kp43 HLA class I, NKG2-A, p39 + – + – – – – – – CD221 Insulin-like growth factor 1 (IGF-I), IGF-II, Insulin JTK13 + + + + + + + + + CD1c M241, R7, T6, Leu6, BDCA1 b-2-Microglobulin + + + – + – – – CD178, FASLG, APO-1, FAS, TNFRSF6, CD95L, APT1LG1, APT1, FAS1, FASTM, CD95 CD178 (Fas ligand) + + + + + – – IGF-II, TGF-b latency-associated peptide (LAP), Proliferin, Prorenin, Plasminogen, ALPS1A, TNFSF6, FASL Cation-independent mannose-6-phosphate receptor (M6P-R, CIM6PR, CIMPR, CI- CD1d R3G1, R3 b-2-Microglobulin, MHC II CD222 Leukemia
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LECTURE 07 Immune Cells Surface Receptors and Their Functions
LECTURE: 07 Title: IMMUNE CELL SURFACE RECEPTORS AND THEIR FUNCTIONS LEARNING OBJECTIVES: The student should be able to: • The chemical nature of the cellular surface receptors. • Define the location of the cellular receptors. • Enumerate the different given terms for the word "receptor". • Define the term "CD" marker. • Describe the location of the immune receptors. • Classify the cellular receptors according to the information they offer. • Classify the cellular receptors according to their families. • Enumerate some common immunoglobulin receptors. • Enumerate some common complement receptors. • Enumerate some adhesion molecules receptors. • Enumerate the main functions of the immune cell receptors. • Realize the importance of the immune receptors. LECTURE REFRENCE: 1. TEXTBOOK: ROITT, BROSTOFF, MALE IMMUNOLOGY. 6th edition. pp. 29, 143, 76, 77, 78, 221, 325. 2. TEXTBOOK: ABUL K. ABBAS. ANDREW H. LICHTMAN. CELLULAR AND MOLECULAR IMMUNOLOGY. 5TH EDITION. Chapter. 6 .pg 105. Chapter 4. pg 65- 80. 1 Lymphocytes can be identified by characteristic markers Lymphocytes and other leukocytes express different surface molecules which can be used for distinguishing cell population. A systemic nomenclature called the CD system has been developed, in which the term CD refers to (cluster of designation). These different cell surface molecules are detected with specific monoclonal antibodies, each of these molecules are given CD number. Molecular markers can be further defined according to the information they offer about the cell. For example; A. Lineage markers are exclusive to a particular cell line, e.g., CD3, which is found only on T cells. B. Maturation markers are transiently expressed during cell differentiation, e.g., CD1, which is only found on cells developing in thymus and is not present on peripheral T cells.
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