DOCSLIB.ORG

Burkholderia Cepacia Complex Remains a Aeruginosa Strains Remain Viable in a Drug Formulation, the Challenge for Drugs Manufacturers

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Burkholderia Cepacia Complex Remains a Aeruginosa Strains Remain Viable in a Drug Formulation, the Challenge for Drugs Manufacturers RESULTS AND DISCUSSION CONTINUED RESULTS AND DISCUSSION CONTINUED RESULTS AND DISCUSSION CONTINUED CONCLUSIONS A New Chromogenic Culture Medium for Screening for The data in this table demonstrates when B. cepacia or P. The exclusion of Burkholderia cepacia complex remains a aeruginosa strains remain viable in a drug formulation, the challenge for drugs manufacturers. CGMP regulations, i.e. Burkholderia cepacia in Non-sterile Pharmaceutical Products ChromID® Burkholderia cepacia medium is able to recover and 21 CFR 211.113(a) recommends appropriate procedures to detect them. prevent the risk of objectionable microorganism contamination. F. BERRAT, M. LARCON, C. de la FOATA and L. LEBLANC bioMérieux Inc. 376 chemin de L’Orme. F-69280 Marcy-L’étoile France In addition, adequately designed contamination control must The data also demonstrates a significantly higher resistance be implemented by pharmaceutical companies to exclude of B. cepacia to preservatives compared to P. aeruginosa. As ABSTRACT MATERIAL AND METHODS CONTINUED potentially harmful microorganisms from their finished goods. ® a consequence, B. cepacia remains a concern and should be The application of a novel chromogenic solid agar ChromID Burkholderia cepacia medium for microbiological examination testing of non- These non-sterile products were artificially contaminated by Currently, no dedicated solution is available to easily cultivate and detected to determine the performance of preservative efficacy. sterile drug products is presented. The propriety medium may be used for both the Total Aerobic Microbial Count as described in USP <61> inoculation with Burkholderia cepacia and Pseudomonas detect B. cepacia complex in pharmaceutical materials.2 and a test for the absence of Burkholderia cepacia complex. As an example, the benefit of the ChromID® Burkholderia aeruginosa (less than 100 CFU/mL). With the introduction of the new culture media ChromID® cepacia medium performance is illustrated in the 2 pictures Burkholderia cepacia, bioMérieux brings an efficient and RESULTS AND DISCUSSION below. The natural flora of the same batch of 2 transdermal INTRODUCTION effective solution to the pharmaceutical industry with patches, artificially contaminated by a suspension of less than Large panel of B. cepacia Strains following characteristics: The U.S. CGMP regulations 21 CFR 211.113 Control of Microbiological Contamination require that objectionable microorganisms be excluded 100 CFU of Burkholderia cepacia 7607022, are grown on TSA In order to control the presence of Burkholderia cepacia complex from non-sterile drug products. An industry survey of non-sterile drug product recalls for microbial contamination between 2004 and 2011 and the ChromID® Burkholderia cepacia medium. • Equivalent performance as a gold standard TSA for a TAMC control (BCC) in a product, the FDA publication recommends that “the highlighted that 34% of the recalls were for Burkholderia cepacia1. FDA microbiologists responded by publishing an article expressing their • Specific and rapid detection of Burkholderia cepacia complex directly on test method validation (to demonstrate the drug is free of BCC) an agar plate suitable for microbial count concern with this opportunistic bacterial pathogen due to its ability to survive in disinfectants, sanitizing agents and preserved, aqueous non- Figure 2: Details of recovery rates observed on Figure 4: Coloration intensity detail observed for Pseudomonas should address multiple strains of the species”1. The in-house Pseudomonas sterile drug products. Of additional concern is the ability of organisms like B cepacia to cause infections in compromised patient populations aeruginosa and Burkholderia spp. population. aeruginosa and Burkholderia spp. Population. • Compatibility with the test methods described in USP <61>, <62> including people with cystic fibrosis. Therefore, the FDA has recommended that microbiological testing methods be capable of screening for Burkholderia strains are presented at the bottom of the poster. 2 For the majority of the targeted species, the specific coloration Burkholderia cepacia . The exclusion of objectionable microorganisms from non-sterile drug product was the topic of a recently published PDA Furthermore, due to the chromogenic activity, the strains of 3 Growth promotion and specific coloration evaluation observed has a score of 3 that corresponds according to the technical report . Pseudomonas aeruginosa and Burkholderia cepacia were readily REFERENCES scale at a great intensity of coloration. The growth promoting capability of this medium to perform As the compendial tests described in USP <61> and <62> do not contain a specific test for the absence of Burkholderia cepacia, bioMérieux detected. Isolated colonies of these microorganisms show a pink 1. Memorandum of the Department Of Health And ® TAMC test was determined. The performance of ChromID ® ® developed an innovative proprietary chromogenic solid medium suitable for both the Total Aerobic Microbial Count and the screening for the to purple color on the ChromID Burkholderia cepacia medium: This demonstrate that the ChromID Burkholderia cepacia Human Services - Public Health Service - Food and Drug Burkholderia cepacia medium is equivalent to TSA and can Gram-negative bacteria Pseudomonas aeruginosa and Burkholderia cepacia in non-sterile drug products. medium can specifically detect Pseudomonas aeruginosa and Administration - Center For Drug Evaluation And Research - be used to perform Total Aerobic Microbial Counts. Burkholderia cepacia with a high level of confidence. 2013 - Reference ID: 3417530 and 3317977 - Erika Pfeiler, John Metcalfe. MATERIAL AND METHODS Average of recovery rate on the 106 strains tested Examination of non-sterile products Filter on ChromID® Filter on TSA: 2. PDA Journal of Pharmaceutical Science and Technology Products tested 120% In addition, the FDA clearly warns the pharmaceutical industry Burkholderia cepacia Score Coloration about the implementation of “test methods and acceptance Observation after Observation after September/October 2011 - Burkholderia cepacia: This The following bioMérieux products were tested: 100% 5 days at 30-35ºC 5 days at 30-35ºC 101% 100% criteria to demonstrate that drug products are free of BCC; […] decision is overdue - Lynn Torbeck, Diane Raccasi, Dennis 0 Not pink Orange, yellow, white 99% 97% 97% • ChromID® Burkholderia cepacia medium, Catalog No. 413454 80% E.Guilfoyle, Richard L. Friedman, David Hussong. any validated method capable of detecting BCC organisms Figure 5: Observation of Figure 6: Observation of • Tryptic Soy Agar (TSA), Catalog No. 43011 1 Light pink 60% 1 would be adequate” . the contamination grown on the contamination grown on 3. PDA Technical Report Number 67 Exclusion of Objectionable regular TSA. ChromID® Burkholderia cepacia 2 Pink Pink 40% ® Growth promotion test study and specific coloration evaluation The ChromID Burkholderia cepacia medium allows the medium. Microorganisms from Nonsterile Pharmaceuticals, Medical Visible differentiation of 3 Dark pink, intense pink, red-pink 20% detection of specific BCC and P. aeruginosa strains for the Devices, and Cosmetics October, 2014. During the development of this non-selective chromogenic solid Burkholderia cepacia colonies Visible detection and medium, 106 different strains were tested. These strains were 4 Purple Purple (light or dark) 0 control of different non-sterile drugs. from other bacterial colonies is not enumeration of Burkholderia Pharmacopoeias strains P. aeruginosa (18) and BCC (25) and other Other Gram negative Other Strains (19): 4. American Pharmaceutical Review December 2014 -Recovery (5) other Pseudomonas Burkholderia species (4) rods (29) Gram positive, yeast, possible on this medium. cepacia is easy and rapid sourced from culture collections and from clinical or environmental species (6) mold, anaerobic bacteria ® of Stressed (Acclimated) Burkholderia cepacia Complex Table 1: Colony color and intensity scale To assess the performance of the ChromID Burkholderia on ChromID® Burkholderia samples: Organisms. Julie Barlasov, Scott Sutton and Richard Jakober. Figure 1: Average of recovery rates calculated on the different type of cepacia cepacia medium. Pseudomonas aeruginosa Pseudomonas aeruginosa Burkholderia cepacia medium, different kinds of non-sterile drugs have • 5 quality control strains described in the pharmacopoeia (Bacillus subtilis Microbial examination of non-sterile products microorganisms from the panel of 106 strains. ATCC 10145 ATCC 9027 ATCC 25416 been tested according to the USP <61> recommendation. ATCC 6633, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa The ChromID® Burkholderia cepacia medium enhances the The microbiological examination of selected non-sterile products ATCC 9027, Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC detection of B. cepacia strains within a contaminated matrix. The results presented in the figure 1 strongly support the Non Fatty 16404), has been performed by enumeration using the membrane filtration Fluid in Figure 3: Pictures of 3 different strains with specific coloration of Water-Soluble Product Fatty Transdermal Aerosol excellent growth performance of the culture medium. For Product Insoluble in Product Patch • 19 strains of Pseudomonas aeruginosa (including Pseudomonas aeruginosa method (described in the U.S. Pharmacopoeia chapter <61>). the colonies. Upper picture: view from above. Bottom picture: view Form the different kind of microorganisms
Load more

Recommended publications
  • Biocontrol of Sclerotinia Stem Rot of Canola by Bacterial Antagonists and Study of Biocontrol Mechanisms Involved
    BIOCONTROL OF SCLEROTINIA STEM ROT OF CANOLA BY BACTERIAL ANTAGONISTS AND STUDY OF BIOCONTROL MECHANISMS INVOLVED by Yilan Zhang A Thesis Submitted to the Faculty of Graduate Studies In Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE Department of Plant Science University of Manitoba Winnipeg, Manitoba, Canada © Yilan Zhang 2004 THE UNIVERSITY OF MANITOBA FACULTY OF GRADUATE STUDIES ***** COPYRIGHT PERMISSION PAGE Biocontrol of Sclerotinia Stem Rot of Canola by Bacterial Antagonists and Study of Biocontrol Mechanisms Involved By Yilan Zhang A Thesis/Practicum submitted to the Faculty of Graduate Studies of the University of Manitoba in partial fulfillment of the requirements of the degree of Master of Science Yilan Zhang © 2004 Permission has been granted to the Library of the University of Manitoba to lend or sell copies of the this thesis/practicum, to the National Library of Canada to microfilm this thesis and to lend or sell copies of the film, and to the Univesity Microfilm Inc. to publish an abstract of this thesis/practicum. The author reserves other publication rights, and neither this thesis/practicum nor extensive extracts from it may be printed or otherwise reproduced without the author’s written permission. ACKNOWLEGEMENTS Fist of all, I would like to express my appreciation to my supervisor Dr. Dilantha Fernando for his guidance in my experiments, and support given at conference presentations and applying for several travel awards. Only with your patience, kindness and encouragement I could grow as a student and plant pathologist. I also appreciate the help of my co-supervisor Dr. Fouad Daayf, for the advice and support for my project during my Masters program.
    [Show full text]
  • Burkholderia Pyrrocinia JK-SH007 Enhanced Seed Germination, Cucumber Seedling Growth and Tomato Fruit Via Catecholate- Siderophore-Mediation
    INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY ISSN Print: 1560–8530; ISSN Online: 1814–9596 19F–065/2019/22–4–779–786 DOI: 10.17957/IJAB/15.1130 http://www.fspublishers.org Full Length Article Burkholderia pyrrocinia JK-SH007 Enhanced Seed Germination, Cucumber Seedling Growth and Tomato Fruit via Catecholate- Siderophore-Mediation Li-Jing Min1,2, Xiao-Qin Wu1, De-Wei Li1,3, Ke Chen4, Lu Guo5 and Jian-Ren Ye1* 1Southern China Collaborative Innovation Center of Sustainable Forestry, College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China 2College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China 3The Connecticut Agricultural Experiment Station Valley Laboratory, Windsor, CT 06095, USA 4Huzhou Institute for Food and Drug Control, Huzhou, Zhejiang 313000, China 5Huzhou First People’s Hospital, Huzhou University, Huzhou, Zhejiang 313000, China *For correspondence: [email protected] Abstract Plant growth-promoting bacteria (PGPB), both endophytic and rhizotrophic bacteria, can play a beneficial role for the biocontrol of phytopathogens and promotion of plant growth. Burkholderia pyrrocinia JK-SH007, as a poplar endophyte, showed strong antagonistic ability to poplar canker disease. A Siderophore involved in the transport of iron in plants and antifungal activities, inferred that siderophore may be the key factor of JK-SH007 biological control of poplar canker disease and plant-growth-promoting even in non-host plant. The siderophore-producing characteristics and conditions of B. pyrrocinia JK-SH007 were investigated, and the impact of JK-SH007-siderophore on plant growth was examined. Chemical analysis indicated that JK-SH007-siderophore was of catecholate-type, as confirmed by HPLC and LC/MS.
    [Show full text]
  • Insights Into the Pathogenicity of Burkholderia Pseudomallei
    REVIEWS Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei W. Joost Wiersinga*, Tom van der Poll*, Nicholas J. White‡§, Nicholas P. Day‡§ and Sharon J. Peacock‡§ Abstract | Burkholderia pseudomallei is a potential bioterror agent and the causative agent of melioidosis, a severe disease that is endemic in areas of Southeast Asia and Northern Australia. Infection is often associated with bacterial dissemination to distant sites, and there are many possible disease manifestations, with melioidosis septic shock being the most severe. Eradication of the organism following infection is difficult, with a slow fever-clearance time, the need for prolonged antibiotic therapy and a high rate of relapse if therapy is not completed. Mortality from melioidosis septic shock remains high despite appropriate antimicrobial therapy. Prevention of disease and a reduction in mortality and the rate of relapse are priority areas for future research efforts. Studying how the disease is acquired and the host–pathogen interactions involved will underpin these efforts; this review presents an overview of current knowledge in these areas, highlighting key topics for evaluation. Melioidosis is a serious disease caused by the aerobic, rifamycins, colistin and aminoglycosides), but is usually Gram-negative soil-dwelling bacillus Burkholderia pseu- susceptible to amoxicillin-clavulanate, chloramphenicol, domallei and is most common in Southeast Asia and doxycycline, trimethoprim-sulphamethoxazole, ureido- Northern Australia. Melioidosis is responsible for 20% of penicillins, ceftazidime and carbapenems2,4. Treatment all community-acquired septicaemias and 40% of sepsis- is required for 20 weeks and is divided into intravenous related mortality in northeast Thailand. Reported cases are and oral phases2,4. Initial intravenous therapy is given likely to represent ‘the tip of the iceberg’1,2, as confirmation for 10–14 days; ceftazidime or a carbapenem are the of disease depends on bacterial isolation, a technique that drugs of choice.
    [Show full text]
  • Functional Identification of the Prnabcd Operon and Its Regulation
    Manuscript Click here to download Manuscript manuscript2366.docx Click here to view linked References Functional identification of the prnABCD operon and its regulation in 1 2 Serratia plymuthica 3 4 5 6 Xiaoguang Liu1*, Xiaoli Yu1, Yang Yang1, Stephan Heeb2, Shao Gao3, Kok Gan Chan4, 7 8 2 5 9 Miguel Cámara , Kexiang Gao 10 11 12 13 1 Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China, 14 15 2 Centre for Biomolecular Sciences, School of Life Sciences, The University of Nottingham, University Park, NG7 2RD, 16 17 United Kingdom 18 19 3 Department of Architecture and Built Environment, Faculty of Engineering, The University of Nottingham, University 20 21 Park, NG7 2RD, United Kingdom 22 23 4 Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 24 25 Kuala Lumpur 50603, Malaysia 26 27 5 Department of Plant Pathology, Shandong Agricultural University, Tai’an 217018, China 28 29 * Correspondence to Xiaoguang Liu [email protected]; Tel: +8651188791702; Fax: +865118791923; Institute of 30 31 Life Sciences, Jiangsu University, Zhenjiang, 212013, China 32 33 34 35 Abstract 36 37 38 39 The antibiotic pyrrolnitrin (PRN) is a tryptophan-derived secondary metabolite that plays an 40 41 important role in the biocontrol of plant diseases due to its broad-spectrum of antimicrobial 42 43 activities. The PRN biosynthetic gene cluster remains to be characterized in Serratia 44 45 plymuthica, though it is highly conserved in PRN-producing bacteria. To better understand 46 47 PRN biosynthesis and its regulation in Serratia, the prnABCD operon from S.
    [Show full text]
  • Targeting the Burkholderia Cepacia Complex
    viruses Review Advances in Phage Therapy: Targeting the Burkholderia cepacia Complex Philip Lauman and Jonathan J. Dennis * Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada; [email protected] * Correspondence: [email protected]; Tel.: +1-780-492-2529 Abstract: The increasing prevalence and worldwide distribution of multidrug-resistant bacterial pathogens is an imminent danger to public health and threatens virtually all aspects of modern medicine. Particularly concerning, yet insufficiently addressed, are the members of the Burkholderia cepacia complex (Bcc), a group of at least twenty opportunistic, hospital-transmitted, and notoriously drug-resistant species, which infect and cause morbidity in patients who are immunocompromised and those afflicted with chronic illnesses, including cystic fibrosis (CF) and chronic granulomatous disease (CGD). One potential solution to the antimicrobial resistance crisis is phage therapy—the use of phages for the treatment of bacterial infections. Although phage therapy has a long and somewhat checkered history, an impressive volume of modern research has been amassed in the past decades to show that when applied through specific, scientifically supported treatment strategies, phage therapy is highly efficacious and is a promising avenue against drug-resistant and difficult-to-treat pathogens, such as the Bcc. In this review, we discuss the clinical significance of the Bcc, the advantages of phage therapy, and the theoretical and clinical advancements made in phage therapy in general over the past decades, and apply these concepts specifically to the nascent, but growing and rapidly developing, field of Bcc phage therapy. Keywords: Burkholderia cepacia complex (Bcc); bacteria; pathogenesis; antibiotic resistance; bacterio- phages; phages; phage therapy; phage therapy treatment strategies; Bcc phage therapy Citation: Lauman, P.; Dennis, J.J.
    [Show full text]
  • Broad-Spectrum Antimicrobial Activity by Burkholderia Cenocepacia Tatl-371, a Strain Isolated from the Tomato Rhizosphere
    RESEARCH ARTICLE Rojas-Rojas et al., Microbiology DOI 10.1099/mic.0.000675 Broad-spectrum antimicrobial activity by Burkholderia cenocepacia TAtl-371, a strain isolated from the tomato rhizosphere Fernando Uriel Rojas-Rojas,1 Anuar Salazar-Gómez,1 María Elena Vargas-Díaz,1 María Soledad Vasquez-Murrieta, 1 Ann M. Hirsch,2,3 Rene De Mot,4 Maarten G. K. Ghequire,4 J. Antonio Ibarra1,* and Paulina Estrada-de los Santos1,* Abstract The Burkholderia cepacia complex (Bcc) comprises a group of 24 species, many of which are opportunistic pathogens of immunocompromised patients and also are widely distributed in agricultural soils. Several Bcc strains synthesize strain- specific antagonistic compounds. In this study, the broad killing activity of B. cenocepacia TAtl-371, a Bcc strain isolated from the tomato rhizosphere, was characterized. This strain exhibits a remarkable antagonism against bacteria, yeast and fungi including other Bcc strains, multidrug-resistant human pathogens and plant pathogens. Genome analysis of strain TAtl-371 revealed several genes involved in the production of antagonistic compounds: siderophores, bacteriocins and hydrolytic enzymes. In pursuit of these activities, we observed growth inhibition of Candida glabrata and Paraburkholderia phenazinium that was dependent on the iron concentration in the medium, suggesting the involvement of siderophores. This strain also produces a previously described lectin-like bacteriocin (LlpA88) and here this was shown to inhibit only Bcc strains but no other bacteria. Moreover, a compound with an m/z 391.2845 with antagonistic activity against Tatumella terrea SHS 2008T was isolated from the TAtl-371 culture supernatant. This strain also contains a phage-tail-like bacteriocin (tailocin) and two chitinases, but the activity of these compounds was not detected.
    [Show full text]
  • Phenotypic and Genetic Diversity of Pseudomonads
    PHENOTYPIC AND GENETIC DIVERSITY OF PSEUDOMONADS ASSOCIATED WITH THE ROOTS OF FIELD-GROWN CANOLA A Thesis Submitted to the College of Graduate Studies and Research In Partial Fulfillment of the Requirements For the Degree of Doctor of Philosophy In the Department of Applied Microbiology and Food Science University of Saskatchewan Saskatoon By Danielle Lynn Marie Hirkala © Copyright Danielle Lynn Marie Hirkala, November 2006. All rights reserved. PERMISSION TO USE In presenting this thesis in partial fulfilment of the requirements for a Postgraduate degree from the University of Saskatchewan, I agree that the Libraries of this University may make it freely available for inspection. I further agree that permission for copying of this thesis in any manner, in whole or in part, for scholarly purposes may be granted by the professor or professors who supervised my thesis work or, in their absence, by the Head of the Department or the Dean of the College in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that due recognition shall be given to me and to the University of Saskatchewan in any scholarly use which may be made of any material in my thesis. Requests for permission to copy or to make other use of material in this thesis in whole or part should be addressed to: Head of the Department of Applied Microbiology and Food Science University of Saskatchewan Saskatoon, Saskatchewan, S7N 5A8 i ABSTRACT Pseudomonads, particularly the fluorescent pseudomonads, are common rhizosphere bacteria accounting for a significant portion of the culturable rhizosphere bacteria.
    [Show full text]
  • Horizontal Gene Transfer to a Defensive Symbiont with a Reduced Genome
    bioRxiv preprint doi: https://doi.org/10.1101/780619; this version posted September 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Horizontal gene transfer to a defensive symbiont with a reduced genome 2 amongst a multipartite beetle microbiome 3 Samantha C. Waterwortha, Laura V. Flórezb, Evan R. Reesa, Christian Hertweckc,d, 4 Martin Kaltenpothb and Jason C. Kwana# 5 6 Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin- 7 Madison, Madison, Wisconsin, USAa 8 Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, 9 Johannes Gutenburg University, Mainz, Germanyb 10 Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research 11 and Infection Biology, Jena, Germanyc 12 Department of Natural Product Chemistry, Friedrich Schiller University, Jena, Germanyd 13 14 #Address correspondence to Jason C. Kwan, [email protected] 15 16 17 18 1 bioRxiv preprint doi: https://doi.org/10.1101/780619; this version posted September 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 19 ABSTRACT 20 The loss of functions required for independent life when living within a host gives rise to 21 reduced genomes in obligate bacterial symbionts. Although this phenomenon can be 22 explained by existing evolutionary models, its initiation is not well understood.
    [Show full text]
  • Molecular Study of the Burkholderia Cenocepacia Division Cell Wall Operon and Ftsz Interactome As Targets for New Drugs
    Università degli Studi di Pavia Dipartimento di Biologia e Biotecnologie “L. Spallanzani” Molecular study of the Burkholderia cenocepacia division cell wall operon and FtsZ interactome as targets for new drugs Gabriele Trespidi Dottorato di Ricerca in Genetica, Biologia Molecolare e Cellulare XXXIII Ciclo – A.A. 2017-2020 Università degli Studi di Pavia Dipartimento di Biologia e Biotecnologie “L. Spallanzani” Molecular study of the Burkholderia cenocepacia division cell wall operon and FtsZ interactome as targets for new drugs Gabriele Trespidi Supervised by Prof. Edda De Rossi Dottorato di Ricerca in Genetica, Biologia Molecolare e Cellulare XXXIII Ciclo – A.A. 2017-2020 Cover image: representation of the Escherichia coli division machinery. Created by Ornella Trespidi A tutta la mia famiglia e in particolare a Diletta Table of contents Abstract ..................................................................................................... 1 Abbreviations ............................................................................................ 3 1. Introduction ........................................................................................... 5 1.1 Cystic fibrosis 5 1.1.1 CFTR defects in CF 5 1.1.2 Pathophysiology of CF 7 1.1.3 CFTR modulator therapies 11 1.2 CF-associated lung infections 12 1.2.1 Pseudomonas aeruginosa 13 1.2.2 Staphylococcus aureus 14 1.2.3 Stenotrophomonas maltophilia 15 1.2.4 Achromobacter xylosoxidans 16 1.2.5 Non-tuberculous mycobacteria 17 1.3 The genus Burkholderia 18 1.3.1 Burkholderia cepacia complex 20 1.3.2 Bcc species as CF pathogens 22 1.4 Burkholderia cenocepacia 23 1.4.1 B. cenocepacia epidemiology 23 1.4.2 B. cenocepacia genome 25 1.4.3 B. cenocepacia pathogenicity and virulence factors 26 1.4.3.1 Quorum sensing 29 1.4.3.2 Biofilm 32 1.4.4 B.
    [Show full text]
  • Morphological and Physiological Characterization and Biomass Production of Burkholderia Pyrrocinia (BRM 32113)
    Brazilian Journal of Development 41096 ISSN: 2525-8761 Morphological and physiological characterization and biomass production of Burkholderia pyrrocinia (BRM 32113) Caracterização morfológica e fisiológica e produção de biomassa de Burkholderia pyrrocinia (BRM 32113) DOI:10.34117/bjdv7n4-529 Recebimento dos originais: 04/02/2021 Aceitação para publicação: 01/03/2021 Marina Teixeira Arriel Elias Mestre em Agronomia Instituição de atuação atual: Universidade Federal de Goiás Endereço: Rodovia Goiânia/Nova Veneza. Km 0, Goiânia, GO 74001-970, Brasil E-mail: [email protected] Maythsulene Inácio de Sousa Oliveira Mestre em Agronomia Instituição de atuação atual: Faculdade de Farmácia - Universidade Federal de Goiás, R. 240 - Setor Leste Universitário, Goiânia - GO, 74605-170. E-mail: [email protected] Gustavo Andrade Bezerra Mestre em Produção Vegetal Instituição de atuação atual: Universidade Federal de Goiás Endereço: Rodovia Goiânia/Nova Veneza. Km 0, Goiânia, GO 74001-970, Brasil E-mail: [email protected] Marcio Vinicius de Carvalho Barros Côrtes Mestre em Bioquímica Instituição de atuação atual: Embrapa Arroz e Feijão Endereço: km 12 - Zona Rural, GO-462, Santo Antônio de Goiás - GO, 75375-000 E-mail: [email protected] Marta Cristina Corsi de Filippi PhD Plant Pathology & Microbiology Instituição de atuação atual: Embrapa Arroz e Feijão Endereço: km 12 - Zona Rural, GO-462, Santo Antônio de Goiás - GO, 75375-000 E-mail: [email protected] ABSTRACT We aimed to evaluate the viability of agroindustry residues for Burkholderia pyrrocinia (BRM32113) biomass production. BRM32113 was morphological and physiologically characterized; carbon: nitrogen ratio, optimal pH and osmotic conditions were determined and carbon sources profile analyzed.
    [Show full text]
  • Strains from the Burkholderia Cepacia Complex: Relationship to Opportunistic Pathogens1
    Journal of Nematology 35(2):208–211. 2003. © The Society of Nematologists 2003. Strains from the Burkholderia cepacia Complex: Relationship to Opportunistic Pathogens1 Peter Vandamme2 and Eshwar Mahenthiralingam3 Abstract: Burkholderia cepacia-like organisms attract much interest from the agricultural industry as natural promoters of plant growth and biological control agents, and for bioremediation. Some of these organisms, however, cause life-threatening infections, particularly in cystic fibrosis patients for whom this multi-resistant bacterium is a major pathogen. The biodiversity of this group of bacteria is severely underestimated, and current identification procedures are inadequate. Presumed B. cepacia isolates belong to at least nine distinct genomic species (genomovars), referred to collectively as the B. cepacia complex. All these B. cepacia complex genomovars have been isolated from clinical and environmental sources. There are no phenotypic, genomic, or taxonomic grounds to differentiate environmental and clinical strains of the B. cepacia complex or to use the source of isolation to assess the safety of biopesticides containing members of the B. cepacia complex. Key words: Burkholderia cepacia complex, cystic fibrosis, epidemiology, genomovar, strain typing. Burkholderia cepacia is an extremely versatile organism tions caused numerous deaths in cystic fibrosis popula- that is truly considered both a friend and foe to humans tions worldwide and new guidelines were issued to re- (Govan et al., 2000). It is a genuinely ubiquitous organ- duce the risk of B. cepacia acquisition (Govan and ism with soil, water (including antiseptic and pharma- Deretic, 1996). More recently, serious outbreaks with ceutical solutions, and seawater), animals, plants, and fatalities have occurred in non-cystic fibrosis patients humans as niches.
    [Show full text]
  • Presence of the Hmq System and Production of 4-Hydroxy-3-Methyl-2-Alkylquinolines Is
    bioRxiv preprint doi: https://doi.org/10.1101/2020.08.27.269993; this version posted August 27, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 2 3 Presence of the Hmq system and production of 4-hydroxy-3-methyl-2-alkylquinolines is 4 heterogeneously distributed between Burkholderia cepacia complex species and more 5 prevalent among environmental than clinical isolates 6 7 Pauline M.L. Coulon1, James E.A. Zlosnik2, & Eric Déziel1* 8 9 1Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique 10 (INRS), Laval, QC, Canada 11 2Canadian Burkholderia cepacia Complex Research and Referral Repository, University of 12 British Columbia, Vancouver, BC, Canada 13 14 *Corresponding author 15 E-mail: [email protected] 16 17 Author contributions 18 PC: conception and design of the manuscript and acquisition of data; JZ: provision and 19 identification of 200 clinical Bcc; ED: conception of the project, resources and funding; PC, JZ 20 and ED: analysis and interpretation of data, revision of the manuscript. 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.08.27.269993; this version posted August 27, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 21 Abstract 22 Some Burkholderia cepacia complex (Bcc) strains have been reported to produce 4- 23 hydroxy-3-methyl-2-alkylquinolines (HMAQs), analogous to the 4-hydroxy-2-alkylquinolines of 24 Pseudomonas aeruginosa.
    [Show full text]