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Data may be preliminary. ta.19) oee,cmeiinfo nohrssc sbrsadmmasmgtas otiueto contribute also might mammals and birds as year-round Samson such 1973; endotherms of cloud Janzen montane from combination 1917; of in competition (Wheeler the 25% However, rare ants that for so only 1997). unsuitable suggested are to forests significant have ants al. cloud compared why a studies et make humidity of mountains is Previous and question wet there temperatures the unanswered. cool of Still, i.e. largely 83% diversity, 2016). remains on ant McCain forests for diversity and affect support (Szewczyk ant to some mountains and interact found arid gradients precipitation temperature elevational and between along temperature relationship patterns diversity whereby explanatory important species model an ant a More there. be of taxa could 2012). meta-analysis 2014) other Kotze recent many and al. A Noreika of et diversity 1997; (Longino high forests al. beetles, the cloud et staphylinid for in Halaj as factor ants 1990; such of patterns Wilson groups, shown near-absence and have the arthropod studies (Holldobler generally, Other other and and Philippines. ants the beetles responsible in between be carabid forests may patterns cloud ants diversity in with diversity complementary a mammal competition of along small suggested rodents in (2001) found and peak Heaney link who ants the Likewise (1977) similar seed-eating for US. Davidson in A the & precipitation in Himalaya. Brown annual gradient by to eastern north-south recorded response the from was in in competition patterns patterns diversity that diversity diversity 2011) idea complementary songbird and the al. ants in support with et peak lend (Acharya competition results mid-elevational density between our to and Overall, contribute diversity including 2019). could plant factors, al. ants 2011), other et from (Supriya al. contributions partly seasonality et exclude higher is (Acharya not Himalaya and productivity does eastern this primary the but in in al. predation, mid-elevations differences ant at et reduced abundance Rosumek of arthropod 2009; consequence high or a Holland relatively occupied and the Chamberlain that plants suggest 1994; from We opportunistic al. arthropods 2010). or et other facultative al. Fiala Even deter et 1977; 2016). Trager and (Bentley 2009; al. herbivory ants et reduce by sites) Chomicki nesting frequently 2007; to this visited (i.e. of known al. in is domatia et risk are Webber and found the ants interactions nectaries 1989; also reduce weaver extra-floral ant-plant we to al. bodies, of ants et as food Fiala with presence 2004a), as 1966; associations such the (Janzen mutualistic al. rewards on strong forests, them et form avoided offering populations mangrove (Offenberg by species species herbivory pest In plant beetle to many leaf reduce 2008; broadly, due a them. significantly More Mele that damage on study. showed ants Van leaf (2004b) weaver pheromones with 2005; used al. that ant correlated Christian still et weaver negatively found Offenberg are and with review 2019). and Peng leaves recent agent al. 1992; eating et A control Khoo (Thurman biological crops and 2019). a tree (Way tropical as demon- orchards al. used been in et have long ants populations Thurman been weaver pest have with trees control ants on weaver to arthropods Asian large along previously. of items guilds abundance strated dietary dietary reduced of of and patterns frequency herbivory diversity and Reduced the identity understand al. the sequencing to et on and useful (Pompanon information very PCR items 2011; that be gradients. to dietary think al. can environmental Due of we methods quantity et Still, these 2018). the (Razgour using 2012). on al. obtained information composition al. et accurate et diet Arrizabalaga-Escudero give Shokralla increasingly 2015; on not 2012; are may al. information methods species et taxonomic these Kartzinel among biases, fine-scale 2014; partitioning al. get niche et overlap to insectivorous dietary niche Brown and dietary tools of species examine ant Studies molecular to an analyses organisms. between using diet diet molecular related in of overlap S1), distantly utility quantified the table between many has demonstrates (Supplementary While that also ants levels. study work and taxonomic Our first birds higher songbirds. the between at be competition birds to taxonomic mid-elevation for all appears and at this evidence birds ants presented elevation weaver have low of and studies diet ants previous weaver the at of in diet birds overlap the for and in availability levels overlap showed food analyses reduce diet ants molecular ants elevations Our weaver weaver these Himalaya. at Since that eastern arthropods removal. suggest fewer the ant results of in weaver these presence elevations after Together, the low to abundance up. contribute in higher could than mos 6 Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. cay,B . .J adr,L iaa,adB htr.21.Eeainlgainsi iddvriyin diversity bird ONE PLoS in gradients mechanisms. Elevational underlying their 2011. and Chettri. patterns Finches distribution B. Ground of and evaluation Vijayan, 6:e29097. Galapagos An L. of Himalaya: Sanders, eastern repository Ecology J. Ecol. the Comparative N. Competition. K., Interspecific B. 1977. and Acharya, github Diversity Grant. Floristic of R. Importance P. 47:151–184. the and Monogr. a of Evaluation Abbott, Gould): K. (Geospiza L. on therein. I., performed Abbott, work manuscript. the the for available accountable revised analyses held cited critically be data Literature work, T.D.P to lab agree and molecular is and C.S.M. fieldwork, publication out for carried manuscript. approval K.S. the study. analyses drafted the designed and and conceived authors for All contributions used Authors interests competing no have authors code The interests and Competing data (https://doi.org/10.5281/zenodo.2651358) Department Chicago Forest All Bengal of West material University the and the from code at fieldwork Data, our Committee for Use permission and 2702/4R-6/2015). received Care and also (1423/WL/4R-1(Part-XII)/2014 We Institutional 71393). by # approved (ACUP reviewers was anonymous Schlumberger study two the This thank from to manuscript. fellowship like Science this Future also in of would the Women version from We Ethics for Museum previous grant Faculty a manuscript. Field on Explorer this a a comments Young writing and by their of a supported collection for time by was data the Research Supriya funded of Student at K. was Foundation Graduate time for collection the Supriya. Fund K. Data Hinds at Amruta Henry Fellowship to to and work. Biology grateful Grant Evolutionary lab Small are Wrayin Rufford We with Brian a Feldheim, Society, help field. Kevin Pranav Geographic for the Khan, National Chanda, Green Anubhab in Ritobroto Nair, Stefan assistance Jyothi Suresh Das, for and Tamma, Brar, Krishnapriya Priyanka Suresh Dey Thatte, Chhetri, Shankar, Abhijit Amir Prachi the Varudkar, and thank Vinod into Bawalia to insights Varughese, like original Sumit his also Jobin for would Balasubramanian, We Karmarkar, Hooper Daniel birds. Cathy Deepika and and on MS, Jablonski ants Rana, the David of of discussions, effects version for earlier competitive lab an Jablonski possible on and comments lab their Price for lab, Pfister Moreau the of of members effect thank We the al. enhance et (Davis shaping or involved in 2007) actors the Acknowledgements taxa on al. related depending is et distantly distributions, it range (Suttle between and change, dampen interactions 1998). abundance climate could species ecological to on interactions on due change these climate studies shift because more competing taxa prey potentially patterns, of for but arthropod related diversity patterns advocate distantly for such diversity We of compete As patterns compare ants taxa. and Himalaya. and patterns these eastern birds monitor the to that shaping wet important in suggest in and elevations data effects warm low experimental biotic needed. the at much and versus in are observational abiotic birds gradients our of affect elevational Overall, along ants importance abundance the postulate ant we compare of that to pattern way tests the same Experimental the elevations. much lower in distributions, ant limiting 7 l uhr aefinal gave authors All Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. adn .J,M .Hne,adB .Edr.21.Ciaecag n nivsv,toia milkweed: tropical invasive, an and change Climate 99:1031–1038. 2018. Ecology Elderd. butterflies. D. monarch B. for for and trap (L.) Hunter, ecological and D. clangula an Rose, M. Bucephala goldeneyes J., De and M. F. Faldyn, fish 434:1015–1017. freshwater Nature a. between 41:99–107. speciation. Competition C. Oecologia drive prey. Giongo, 1979. can common diversity G. A. high Species O. with Lange, M. 2005. P. 12:1450013. analysis Eriksson, Kolm. Biol. metagenomic N. a. Comput. and Improving when T. C., Bioinform. NCBI-TaxCollector: mistakes B. Neves, J. Emerson, Making and attachment. V. taxonomic MPI-blastn M. wide 1998. Rossi, – and 2014. classification D. evolution Wood. performance F. S. of Triplett. 391:783–786. Xavier, and masterpiece Nature W. G. Shorrocks, A warming. E. M. B. global to 2009. Lawton, R., response H. Dias, in Robson. J. range A. species Jenkinson, in K. S. shifts S. L. predicting and J., 13:57–71. Schluns, A. News A. Davis, Myrmecol. E. Formicidae). in Newey, (Hymenoptera: insects: S. ants for 14:1160–1170. weaver P. metabarcodes Oecophylla Resour. Environmental H., Ecol. 2014. R. Mol. Cooper. Crozier, bias. A. taxonomic and Weyrich, 211:1358–1370. for concealed S. potential Phytol. through L. reveals choice New Soubrier, Partner silicoPCR mutualisms. J. ant–plant 2016. J., of Renner. L. specialization Clarke, S. 31:343–366. the S. Syst. with and evolved Ecol. Schonenberger, rewards J. Rev. sugar Staedler, floral Annu. M. diversity. Y. species G., of Chomicki, maintenance Venn of highly-customizable Mechanisms of 2000. generation 12:35. P. the Bioinformatics 2016. for Chesson, ant–plant BMC package R. a in Holmes. in VennDiagram: dependency P. diagrams 2011. context Euler S. Boutros. of and and C. synthesis P. Quantitative Johnson, and H., A. Chen, 2009. J. Holland. A. 90:2384–2392. 13:581–583. N. Ecology Han, Methods J. mutualisms. W. Nat. and protection desert A. data. A., in amplicon Rosen, S. ants Illumina J. Chamberlain, from and M. inference rodents sample McMurdie, seed-eating resolution J. between High P. DADA2: Competition J., B. 1977. re-introduced Callahan, a Davidson. and W. Symondson. murinus) 196:880–882. D. C. Science (Suncus O. and ecosystems. 23:3695–3705. Shrew W. H., Ecol. and Past, Musk J. Montazam, Mol. the Asian Brown, A. telfairii). and alien Clare, (Leiolopisma Change L. the Skink Climate Telfair’s E. between Vencatasamy, of D. competition 2013. population Cole, Dietary N. Finnegan. Burger, S. 2014. R. and S., 341:499–504. Ecol. Fitzpatrick, D. Science Brown, C. Rev. Interactions. M. Biotic Zarnetske, Annu. of Future L. and bodyguards. P. Present, forest pugnacious L., evergreen by J. wet protection Blois, a and in nectaries community Extrafloral 8:407–427. 73:173–179. ant Syst. 1977. Sci. foraging L. Curr. ground B. behaviour. the Bentley, interference Goiti. in of Ecol. U. role hierarchy Mol. and India): Competition Ghats, metabarcoding. Aihartza, (Western DNA J. 1997. by P. Garin, species Basu, I. bat Alberdi, sibling co-occurring A. of Salsamendi, partitioning 27:1273–1283. E. 37:406–415. niche Ecography Clare, Assessing interactions. biotic L. niches 2018. of E. species scaling geographic of A., The models Arrizabalaga-Escudero, 2014. in Rozenfeld. considered A. and be B., interactions M. biotic Araujo, 44:8–17. should how Biogeogr. and J. When distributions? and 2017. P. R. Anderson, 8 Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. azn .H 93 we ape ftoia oig net:Eet fsaos eeaintps elevation, types, vegetation seasons, of Evolution Effects 54:687–708. Ecology insects: America. foliage insularity. Central tropical and of day, in samples of acacias Sweep time and 1973. H. ants D. between Janzen, may mutualism aggression of Interspecific Coevolution top: the 1966. 20:249–275. at H. Squeezed 91:1877–1884. D. 2010. Ecology Janzen, Levey. birds. J. tropical D. in and and ranges scales Robinson, elevational across K. constrain mismatches S. E., and J. extensions Jankowski, macroevolution: MA. and Cambridge 62:715–739. Press, interactions Evolution Belknap 5:367–371. Biotic levels. ants. Evol. 2008. The Ecol. D. Trends 1990. Jablonski, kingdoms. Wilson. between O. Competition E. and 1990. B., assessment Lawton. Holldobler, an H. J. Philippines: and 10:15–39. the E., in Biogeogr. M. Douglas-fir gradients Ecol. Hochberg, elevational in Glob. spiders along diversity on hypotheses. foraging mammal and ant patterns Small R of of 2001. effects data. R. Negative ecological L. 1997. for Heaney, Moldenke. analysis R. model A. 109:313–322. Null and Oecologia EcoSimR: Ross, canopies. W. 2015. D. J., Ellison. M. Halaj, A. and Hart, 0.1.0. M. version package E. 79:1181–1192. 2010. J., Ecol. Daniel. N. assembly community Carlos Gotelli, Anim. in Cadena J. filtering and habitat birds. and G., forest competition Jose of Tello Neotropical role T., of the Robb disentangling 139:771–801. to Brumfield natural approach Nat. A., in phylogenetic Gustavo A competition Am. Bravo interspecific Pablo, plants. of Juan with consequences Gomez and experiments Patterns field of 1992. review Barton. a M. in A. communities: gradients and 28:125–130. E., elevational Res. D. across 283:20160047. Goldberg, Ecol. foliage-arthropods Sci. Himalayas. of west Biol. abundance macroevolutionary and B and east coexistence: Soc. Distribution the Species R. 2013. Proc. 2016. M. interactions. Ghosh-Harihar, Benjamin. historical Gilbert of contingency and the T., and Jason relationships Weir Ecol. M., Funct. Rachel better). Germain 2015. far Patrick. are Venail (they and mechanisms Andreas, assembly Prinzing community of V., 29:600–614. proxies Igor not Bartish are Marten, biotic patterns Winter for Phylogenetic F., Accounting James Cahill 2017. Evol. Pille, Kunin. Ecol. Gerhold E. Methods W. models. and distribution Haase, species P. stacked Syst. Stoll, lowland 8:1092–1102. in S. Ecol. constraints tropical Kuemmerlen, alpha-diversity Rev. M. in through Annu. Marsh, interactions predators J. Specialization. C. key Ecological Y., of as Gavish, Evolution ants The Arboreal 1988. Moreno. 2002. G. 19:207–233. and ant-plant J., Linsenmair. D. Asian Futuyma, K. East E. South 131:137–144. and Oecologia a trees. of Biun, 14:64–81. rainforest Evolution Studies A. Diversity. Organic A., 1989. in Floren, Variations 79:463–470. Latitudinal Helbig. Oecologia 1960. interactions: J. borneensis. G. ant-plant A. A. of and by Fischer, Diversity trees Pong, Macaranga 97:186–192. Y. of Oecologia 1994. T. protection association. Maschwitz, association: ant Linsenmair. U. of E. degrees B., different K. Fiala, with and species Maschwitz, Macaranga in U. efficacy Grunsky, protective H. B., Fiala, 9 Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. io,J,X saae,N aels .Mrie-iat,J .Brino,adD o.21.Atversus Ant 35:367–376. 2010. Entomol. Ecol. Sol. grove. D. citrus and organic Barrientos, an A. of J. assemblage arthropod Martinez-Vilalta, the J. on on effects Canellas, 14:49–57. effects canopies: N. exclusion Entomol. citrus Espadaler, bird For. from X. ant-exclusion Agric. of J., yield. years Pinol, fruit Eight on 2012. and Canellas. assemblage N. arthropod and the Int. Espadaler, element. X. key J., Territory a Pinol, Northern as the Formicidae) in 51:149–155. (Hymenoptera: orchards , Manag. mediates smaragdina in Pest interactions Oecophylla management J. ant, vocal pest weaver Integrated frugivore via the 2005. dominance using neotropical , 182:E161-173. Interspecific Christian. a K. Nat. 2013. and of Am. R., structure Peng, Phelps. mice. Trophic M. singing S. neotropical in and 1989. zonation Bolker, 79:403–411. altitudinal M. Oecologia Stoleson. B. bats? S. B., and Pasch, birds and between Gorchoy, competition Community Sons. L. there & is vegan: D. Wiley community: John M., 2018. ecosystems. J. Wagner. forest Palmeirim, in H. sampling and Insect Szoecs, 2005. E. C. Stevens, O’Hara, Ozanne, B. H. R. Minchin, Henry, R. P. 271:S433–S435. M. McGlinn, Solymos, Package. Sci. J. D. P. Ecology Biol. Legendre, insect P. Simpson, B Kindt, that L. R. Lond. Friendly, Evidence M. G. Soc. 2004b. Blanchet, G. R. Aksornkoae. F. S. J., Proc. Oksanen, and pheromones. Havanon, ant S. by MacIntosh, J. deterred D. are on Nielsen, herbivores the effect G. on their M. Observations and J., 36:344–351. ecosystem 2004a. Offenberg, Biotropica mangrove Nielsen. Thai Lam. G. a mucronata M. in Rhizophora Fabricius) and of smaragdina Macintosh, herbivory (Oecophylla J. ants D. weaver distribution Aksornkoae, of increasing 16:867–881. spatial S. ecology the through Conserv. Havanon, on Insect S. evolved effect J. J., predictable interactions a Offenberg, forests. have Ant–plant contrasts urban edge in Forest 2018. beetles 2012. carabid Kotze. of Moreau. J. D. S. and 115:12253–12258. C. N., Sci. Noreika, and Acad. Ree, Natl. P. H. Proc. richness. R. interdependence. species P., Chichester. in phylogenetic Ltd, M. gradients the Sons, Elevational Nelsen, & on Wiley 2010. exclusion John competitive Grytnes. (ELS). of Sciences J.-A. effects Life and Opposing M., C. 13:1085–1093. 2010. McCain, Lett. 48:165–174. Levine. Environ. Ecol. Fauna M. communities. composition Neotropical J. community of and Stud. and structure diversity forests. M., beetle Andean Carabid M. in tropical Mayfield, 2013. seasonality on Erwin. and abundance L. altitude ant T. to out: and related drop Browne, as ants A. How R. species A., 2014. joint S. Maveety, Colwell. dynamic K. 9:e104030. R. Preisser. a and ONE L. by Branstetter, PLoS E. G. revealed and mountains. M. Orwig, differences T., A. J. niche D. Longino, Orians, abiotic M. 99:1018–1023. C. and Schaeffer, Ecology interactions N. model. R. biotic distribution Schliep, Asymmetric M. African E. by Zarnetske, partitioning 2018. L. niche Rubenstein, P. 112:8019–8024. I. dietary K., D. Sci. illuminates N. Kuzmina, Lany, metabarcoding L. Acad. M. DNA Natl. Kress, J. 2015. of between Proc. W. roles Pringle. Erickson, herbivores. competition interacting L. M. large D. for R. Coverdale, caterpillars: and C. Evidence more Wang, T. Chen, have W. A. years P. 2010. Wet 98:2370–2378. R., T. Ecology Kartzinel, 2017. Rohr. ants. by R. Holyoak. predation 277:3001–3008. J. M. and Sci. and litter and plant Biol. Grof-Tisza, Raffel, B P. R. R., Soc. R. Karban, T. Proc. Krupa, spiders. J. and J. plants carnivorous E., D. Jennings, 10 in nylpdaof Encyclopedia Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. ukr .M,M .Cdte .B avlo .J ais .Frir .A rt,R rne,M R. M. macroecology. and Grenyer, and Winter, ecology R. M. community Fritz, Rosauer, conservation, A. F. for D. S. metrics Redding, Ferrier, phylogenetic W. S. D. to Davies, Purschke, 92:698–715. guide O. J. A Rev. Pavoine, T. S. Biol. 2017. Carvalho, Mooers, B. O. Mazel. A. S. W. F. Jin, Cadotte, C. S. meta-analysis. McKeon, W. L. a S. M. Helmus, mutualisms: C. protective M., Rodriguez-Cabal, ant-plant C. in A. Tucker, plants M. for Andrade, to Benefits V. Services 5:e14308. 2010. G. ONE Ecosystem Bolker. Hostetler, PLOS Provide M. A. Ants B. Weaver J. and Bhotika, Osenberg, 2019. S. D., Snyder. 7. M. Ecology E. Trager, Evol. W. birds. Ecol. Andean and Front. of Northfield, Crops. distribution D. Tree the Tropical T. in H., competition J. of Thurman, role The 1975. Weske. Diversity. S. Elevational 56:562–576. J. Ant of and Drivers J., Global Terborgh, of to Review responses Systematic grassland A 11:e0155404. reverse 2016. One interactions McCain. Plos Species M. C. 2007. and elevational T., Power. temperate Szewczyk, E. and M. tropical 315:640–642. and of Science Thomsen, Analysis climate. A. changing 2019. M. B., Price. 0. K. D. Biogeogr. Suttle, CATIE. T. / Front. and IICA abundance. Orton Sam, arthropod K. Bib. in Moreau, review. gradients bibliographic S. a C. technologies tropics: K., humid sequencing Supriya, the Next-generation in forests 2012. species Cloud bird Hajibabaei. 1987. 21:1794–1805. of T. M. Ecol. Stadtmuller, driver and the Mol. Gibson, as research. F. 10.1086/707665. limits DNA J. latitudinal doi: environmental Ecological Spall, Nat., a for L. 2019. there Am. J. Is gradient. S., Price. elevational Shokralla, T. 2009. Himalayan 40:245–269. and east Roy. Syst. Supriya, the K. Evol. K. along and patterns Ecol. White, richness Sobel, E. Rev. M. A. Annu. J. M., interactions? Cornell, Schumm, biotic elevational V. of an H. importance along Mittelbach, the abundance in G. and gradient G. diversity Ant W., D. 1997. Schemske, 29:349–363. Gonzales. Biotropica C. n/a-n/a. P. Ecography Philippines. and the Guinea. Rickart, G. in New A. gradient Pezzini, Papua E. caterpillars in F. A., of gradient Oki, predation D. biotic forest ant Samson, Y. and plant elevational avian as complete Diniz, increases damage ants a L. Herbivore of along Barbosa, 2014. role trees Novotny. U. the on V. and de of Koane, P. meta-analysis B. K., a N. Sam, plants: Neves, on S. G. Ants de 160:537–549. and 2009. Oecologia F. Gilbert, Cornelissen. Silveira, defenses. P. T. O. T. and A. Rasmussen, Fernandes, cryptic F. W. M. in B., Schnell, partitioning F. B. resource I. Rosumek, of 1:556–570. Hanmer, mechanisms Evol. into J. Ecol. insight Zeale, offers species. K. sequencing bat R. High-throughput M. Statistical 2011. Clare, for Foundation Jones. L. R E. M. computing. O., Olsson, filling statistical Razgour, Niche U. for environment Alstrom, 2014. and P. language Mohan. Austria. Tietze, A D. Vienna, R: T. Computing, and 2014. D. Singh, Team. P. 509:222–225. Johansson, Core Nature Harr, S. R B. songbirds. U. Martens, Himalayan Buchanan, of J. diversification D. Gupta, the Who K. C. slows 21:1931–1950. S. 2012. Hooper, Ishtiaq, Ecol. Taberlet. M. P. F. Mol. and D. Ghosh-Harihar, Jarman, sequencing. D., N. generation S. T. next Brown, Price, using S. assessment D. Symondson, diet C. what: O. eating W. is Deagle, E. B. F., Pompanon, 11 Posted on Authorea 10 Feb 2020 — CC BY 4.0 — https://doi.org/10.22541/au.158137036.64067629 — This a preprint and has not been peer reviewed. Data may be preliminary. siae n1 evsdsrbtdaon h tree. the around ant distributed leaves and 10 Bird on estimated mid-elevations. not. at did other silhouette. birds (a-b) bird and the 3: elevations produced and Figure Caravaggi low blue Anthony at in phylopic.org. from shown birds taken study ants, silhouettes this weaver in between further 2014) metabarcoding examined al. 2 elevation et (Price 2000m Figure in and data 200m on at Based Site respectively. yellow for gradient. PCR same Taxon-specific the Himalaya 2011. eastern the Jones. in G. a. and 1: 11:236–244. Lees, Figure C. Resour. D. Ecol. Barker, Mol. 8:352–359. A. legends: faeces. J. L. Figure bat R G. in R. Butlin, prey in K. arthropod Data R. barcoding Sequence DNA Biological K., Big realised R. Analyze and M. 88:15–30. to distributions Zeale, v2.0 Rev. shaping DECIPHER Wardle, C. in Biol. Using Maiorano, A. interactions M. modelling. D. L. 2016. biotic Dormann, distribution E. Timmermann, Luoto, of F. Wright, species A. role M. for C. Termansen, The Kuhn, implications Damgaard, M. I. 2013. species: Schmidt, F. Hoye, of M. Svenning. C. assemblages T. N. J.-C. Lenoir, T. Ockinger, and J. Heikkinen, E. Anim. Aastrup, Pellissier, P. K. J. Normand, L. S. R. lecture. Kissling, Nilsson, Guisan, tansley D. M.-C. sixth A. W. The Grytnes, Pottier, ants. J.-A. J. Forchhammer, the S., of case M. the Wisz, success: ecological of 3:180–185. 52:457– 56:1–9. Causes Stat. Sci. Ecol. 1987. Comput. Arts O. Rev. E. Acad. Interdiscip. Wilson, Am. Wiley Proc. ggplot2. 2011. America. H. North Wickham, western of ants mountain The 569. 1917. in interactions M. ant–plant hemipteran W. of 154:353–371. and diversity Wheeler, prostomata, The Soc. domatia, 2007. Linn. bodies, J. Woodrow. food E. Bot. Annu. I. (Achariaceae): and trophobionts. Ryparosa ecology. Curtis, tree, community O. understorey S. and rainforest A. 479–503. the Phylogenies Moog, Entomol. J. 2002. L., Rev. B. Annu. Webber, Donoghue. management. M. 33:475–505. pest and Syst. in Mcpeek, Ecol. ants M. of Rev. Ackerly, Role D. The 1992. mesocosm C., 2014. a Khoo. Webb, C. in Cardinale. K. algae J. and B. green J., freshwater and M. Way, Oakley, among H. interactions 102:1288–1299. T. species Ecol. Alexandrou, J. on A. relatedness experiment. M. Fritschie, phylogenetic Agric. K. of control. Narwani, biological influence A. in Oecophylla A., ant P. weaver Venail, the on research species of 10:13–22. rodent review on Entomol. historical extinction For. A and 2008. colonization, P. competition, Mele, Van of Effects in 1995. interactions A. 267:880–883. Rey, Brown. ecological D. Science J. H. of P. diversity. J. Garcia, extinction Ramirez, and B. the N. J., M. Oviedo, T. loss: 29:299–307. Valone, R. Galetti, species species Ecol. Obeso, Beyond M. and Funct. R. 2015. Cocucci, change J. world. Navarro, A. Zamora. changing Global L. Arroyo, a R. Medel, and J. R. 2008. Verdu, Alcantara, Jordano, M. P. Traveset, M. Wardle. Gomez, J. M. A. Aizen, J. D. Garcia, A. and M. 11:1351–1363. Bascompte, A., Lett. Valiente-Banuet, J. Ecol. Didham, ecosystems. K. terrestrial in R. interactions M., J. 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