bioRxiv preprint doi: https://doi.org/10.1101/780155; this version posted September 23, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Classified Personnel Information 1 High throughput screening and identification of coagulopathic snake venom proteins and 2 peptides using nanofractionation and proteomics approaches 3 4 Julien Slagbooma,b, Marija Mladićc, Chunfang Xie b, Freek Vonkd, Govert W. Somsenb, Nicholas 5 R. Casewella, Jeroen Koolb 6 aCentre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, 7 Liverpool, UK 8 bDivision of BioAnalytical Chemistry, Amsterdam Institute for Molecules Medicines and Systems, 9 VU University Amsterdam, Amsterdam, The Netherlands 10 cAnimal Sciences and Health, Institute of Biology Leiden, Leiden University, Leiden, The 11 Netherlands 12 dNaturalis Biodiversity Center, Leiden, The Netherlands 13 *Corresponding author
[email protected] 14 15 1 bioRxiv preprint doi: https://doi.org/10.1101/780155; this version posted September 23, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Classified Personnel Information 16 Abstract 17 Snakebite is a neglected tropical disease that results in a variety of systemic and local pathologies in 18 envenomed victims and is responsible for around 138,000 deaths every year. Many snake venoms cause 19 severe coagulopathy that makes victims vulnerable to suffering life-threating haemorrhage.