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												  Overcoming the Challenges of Tamarix Management with Diorhabda Carinulata Through the Identification and Application of SemiocheOVERCOMING THE CHALLENGES OF TAMARIX MANAGEMENT WITH DIORHABDA CARINULATA THROUGH THE IDENTIFICATION AND APPLICATION OF SEMIOCHEMICALS by Alexander Michael Gaffke A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Ecology and Environmental Sciences MONTANA STATE UNIVERSITY Bozeman, Montana May 2018 ©COPYRIGHT by Alexander Michael Gaffke 2018 All Rights Reserved ii ACKNOWLEDGEMENTS This project would not have been possible without the unconditional support of my family, Mike, Shelly, and Tony Gaffke. I must thank Dr. Roxie Sporleder for opening my world to the joy of reading. Thanks must also be shared with Dr. Allard Cossé, Dr. Robert Bartelt, Dr. Bruce Zilkowshi, Dr. Richard Petroski, Dr. C. Jack Deloach, Dr. Tom Dudley, and Dr. Dan Bean whose previous work with Tamarix and Diorhabda carinulata set the foundations for this research. I must express my sincerest gratitude to my Advisor Dr. David Weaver, and my committee: Dr. Sharlene Sing, Dr. Bob Peterson and Dr. Dan Bean for their guidance throughout this project. To Megan Hofland and Norma Irish, thanks for keeping me sane. iii TABLE OF CONTENTS 1. INTRODUCTION ...........................................................................................................1 Tamarix ............................................................................................................................1 Taxonomy ................................................................................................................1 Introduction
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												  CDA Leafy Spurge BrochureFrequently Asked Questions About the Palisade Insectary Mission Statement How do I get Aphthona beetles? You can call the Colorado Department of We are striving to develop new, effective Agriculture Insectary in Palisade at (970) ways to control non-native species of plants 464-7916 or toll free at (866) 324-2963 and and insects that have invaded Colorado. get on the request list. We are doing this through the use of biological controls which are natural, non- When are the insects available? toxic, and environmentally friendly. We collect and distribute adult beetles in June and July. The Leafy Spurge Program In Palisade How long will it take for them to control my leafy spurge? The Insectary has been working on leafy Biological Control You can usually see some damage at the spurge bio-control since 1988. Root feeding point of release the following year, but it flea beetles are readily available for release of typically takes three to ten years to get in early summer. Three other insect species widespread control. have been released and populations are growing with the potential for future Leafy Spurge What else do the beetles feed on? distribution. All of the leafy spurge feeding The beetles will feed on leafy spurge and insects are maintained in field colonies. cypress spurge. They were held in Additional research is underway to explore quarantine and tested to ensure they would the potential use of soilborne plant not feed on other plants before they were pathogens as biocontrol agents. imported and released in North America What makes the best release site? A warm dry location with moderate leafy spurge growth is best.
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												  Integrated Noxious Weed Management Plan: US Air Force Academy and Farish Recreation Area, El Paso County, COIntegrated Noxious Weed Management Plan US Air Force Academy and Farish Recreation Area August 2015 CNHP’s mission is to preserve the natural diversity of life by contributing the essential scientific foundation that leads to lasting conservation of Colorado's biological wealth. Colorado Natural Heritage Program Warner College of Natural Resources Colorado State University 1475 Campus Delivery Fort Collins, CO 80523 (970) 491-7331 Report Prepared for: United States Air Force Academy Department of Natural Resources Recommended Citation: Smith, P., S. S. Panjabi, and J. Handwerk. 2015. Integrated Noxious Weed Management Plan: US Air Force Academy and Farish Recreation Area, El Paso County, CO. Colorado Natural Heritage Program, Colorado State University, Fort Collins, Colorado. Front Cover: Documenting weeds at the US Air Force Academy. Photos courtesy of the Colorado Natural Heritage Program © Integrated Noxious Weed Management Plan US Air Force Academy and Farish Recreation Area El Paso County, CO Pam Smith, Susan Spackman Panjabi, and Jill Handwerk Colorado Natural Heritage Program Warner College of Natural Resources Colorado State University Fort Collins, Colorado 80523 August 2015 EXECUTIVE SUMMARY Various federal, state, and local laws, ordinances, orders, and policies require land managers to control noxious weeds. The purpose of this plan is to provide a guide to manage, in the most efficient and effective manner, the noxious weeds on the US Air Force Academy (Academy) and Farish Recreation Area (Farish) over the next 10 years (through 2025), in accordance with their respective integrated natural resources management plans. This plan pertains to the “natural” portions of the Academy and excludes highly developed areas, such as around buildings, recreation fields, and lawns.
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												  New DNA Markers Reveal Presence of Aphthona Species (Coleoptera: Chrysomelidae) Believed to Have Failed to Establish After Release Into Leafy SpurgeBiological Control 49 (2009) 1–5 Contents lists available at ScienceDirect Biological Control journal homepage: www.elsevier.com/locate/ybcon New DNA markers reveal presence of Aphthona species (Coleoptera: Chrysomelidae) believed to have failed to establish after release into leafy spurge R. Roehrdanz a,*, D. Olson b,1, G. Fauske b, R. Bourchier c, A. Cortilet d, S. Sears a a Biosciences Research Laboratory, Red River Valley Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, 1605 Albrecht Blvd, Fargo, ND 58105, United States b Department of Entomology, North Dakota State University, Fargo, ND, United States c Agriculture and Agri-Food Canada, Lethbridge, AB, Canada d Agricultural Resources Management and Development Division - Weed Integrated Pest Management Unit, Minnesota Department of Agriculture, St. Paul, MN, United States article info abstract Article history: Six species of Aphthona flea beetles from Europe have been introduced in North America for the purpose Received 28 September 2007 of controlling a noxious weed, leafy spurge (Euphorbia esula). In the years following the releases, five of Accepted 10 December 2008 the species have been recorded as being established at various locations. There is no evidence that the Available online 31 December 2008 sixth species ever became established. A molecular marker key that can identify the DNA of the five established species is described. The key relies on restriction site differences found in PCR amplicons Keywords: of a portion of the mitochondrial cytochrome oxidase I gene. Three restriction enzymes are required to Flea beetles separate the immature specimens which are not visually separable. Adults which can be quickly sepa- Leafy spurge rated into the two black species and three brown species require only two restriction enzymes to resolve Euphorbia esula Aphthona the species.
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												  (Leafy Spurge), on a Native Plant Euphorbia RobustaNon-target impacts of Aphthona nigriscutis, a biological control agent for Euphorbia esula (leafy spurge), on a native plant Euphorbia robusta John L. Baker, Nancy A.P. Webber and Kim K. Johnson1 Summary Aphthona nigriscutis Foudras, a biological control agent for Euphorbia esula L. (leafy spurge), has been established in Fremont County, Wyoming since 1992. Near one A. nigriscutis release site, a mixed stand of E. esula and a native plant, Euphorbia robusta Engelm., was discovered in 1998. During July of 1999, A. nigriscutis was observed feeding on both E. esula and E. robusta. A total of 31 E. robusta plants were located and marked on about 1.5 ha of land that had an E. esula ground-cover of over 50%. Eighty-seven percent of the E. robusta plants showed adult feeding damage. There was 36% mortality for plants with heavy feeding, 12% mortality for plants with light feeding, and no mortality for plants with no feeding. By August of 2002, the E. esula ground-cover had declined to less than 6% and the E. robusta had increased to 542 plants of which only 14 plants (2.6%) showed any feeding damage. For the four-year period, the E. esula ground-cover was inversely correlated to E. robusta density and posi- tively correlated to A. nigriscutis feeding damage, showing that as E. esula density declines so does Aphthona nigriscutis feeding on E. robusta. Keywords: Aphthona, density, Euphorbia, mortality, non-target impacts. Introduction established populations. These sites were monitored annually to assess the establishment of the bioagents. A parcel of land 4.8 km (3 miles) south-west of Lander, There was a strong contrast between the Majdic land Fremont County, Wyoming has been infested with and the Christiansen properties where the insects were Euphorbia esula (leafy spurge) for over 30 years.
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												  Inundative Release of Aphthona Spp. Flea Beetles (Coleoptera: Chrysomelidae) As a Biological "Herbicide" on Leafy Spurge in Riparian AreasInundative Release of Aphthona spp. Flea Beetles (Coleoptera: Chrysomelidae) as a Biological "Herbicide" on Leafy Spurge in Riparian Areas 5 R. A. PROCAR,l G. MARKIN,2 J. MILAN T. BARBOULETOS,3 AND M. J. RINELLA ABSTRACT Inundative releases of beneficial insects are frequently used to suppress pest insects but not commonly attempted as a method of weed biological control because of the difficulty in obtaining the required large numbers of insects. The successful establishment of a flea beetle complex, mixed Aphthona lacertoea (Rosenhauer) and Aphthona nigriscutus Foundras (87 and 13%, respectively), for the control of leafy spurge, Euphorbia esula L, provided an easily collectable source of these natural enemies that enabled us to attempt inundative release as a possible leafy spurge control method in a sensitive riparian ecological zone where chemical control is restricted. Our target weed populations were small isolated patches of leafy spurge along three streams in southwestern, central, and north- eastern Idaho. This study assessed leafy spurge and associated vegetation responses to Inundative releases of 10 and 50 beetles per spurge flowering stem over two consecutive years. Releasing 10 beetles per flowering stem had inconclusive effects on spurge biomass, crown, stem, and seedling density. Alternatively, releasing 50 beetles per flowering stem resulted in a reduction of biomass, crown and stem density in the range of 60-80% at all three study sites, and about an =60% reduction of seedling density at one site, compared with untreated plots. In contrast to leafy spurge, associated vegetation did not conclusively respond to beetle release, indicating that it may take more than two years for desired riparian vegetation to respond to reductions in leafy spurge competition.
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												  Biology of Aphthona Nigriscutis (Coleoptera: 1 Chrysomelidae) in the LaboratoryReprinted with permission from: Annals of the Entomological Society of America. July 1997. 90(4):433-437. Published and copyrighted by: ©1998 Entomological Society of America. www.entsoc.org Biology of Aphthona nigriscutis (Coleoptera: 1 Chrysomelidae) in the laboratory JAN J. JACKSON Northern Grain Insects Research Laboratory, USDA-ARS, 2923 Medary Avenue, Brookings, SD 57006 Abstract: The flea beetle Aphthona nigriscutis Foudras was introduced to Canada in 1983 and the United States in 1989 for the biological control of leafy spurge, Euphorbia esula L. Life history data for A. nigriscutis are de- scribed based on laboratory studies using insect populations from Barnes County, North Dakota, and Fallon County, Montana. Initial emergence of males and females from spring soil samples was synchronized, but com- plete emergence for males preceded that of females. For the Barnes County population, ≈80% of the adults were female. When adults were held as mated pairs at 25° C and a photoperiod of 14:10 (L:D) h, male lon- gevity was longer than that of the female with a median longevity of 221 and 124 days, respectively. Oviposition started ≈10 days after female emergence and proceeded at a rate of 4 eggs per day over an average ovi- position period of 109 days. Oviposition averaged 537 eggs per female with a range of 103-1,157. Eggs from the Fallon County population started to hatch within 10 days after oviposition when eggs were held at 25° C. Egg hatch averaged near 60% and occurred over a period of ≈8 days. Life history data for A. nigriscutis were similar to other univoltine Aphthona spp., except A.
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												  Forest Health Technology Enterprise Team Biological Control of InvasiveForest Health Technology Enterprise Team TECHNOLOGY TRANSFER Biological Control Biological Control of Invasive Plants in the Eastern United States Roy Van Driesche Bernd Blossey Mark Hoddle Suzanne Lyon Richard Reardon Forest Health Technology Enterprise Team—Morgantown, West Virginia United States Forest FHTET-2002-04 Department of Service August 2002 Agriculture BIOLOGICAL CONTROL OF INVASIVE PLANTS IN THE EASTERN UNITED STATES BIOLOGICAL CONTROL OF INVASIVE PLANTS IN THE EASTERN UNITED STATES Technical Coordinators Roy Van Driesche and Suzanne Lyon Department of Entomology, University of Massachusets, Amherst, MA Bernd Blossey Department of Natural Resources, Cornell University, Ithaca, NY Mark Hoddle Department of Entomology, University of California, Riverside, CA Richard Reardon Forest Health Technology Enterprise Team, USDA, Forest Service, Morgantown, WV USDA Forest Service Publication FHTET-2002-04 ACKNOWLEDGMENTS We thank the authors of the individual chap- We would also like to thank the U.S. Depart- ters for their expertise in reviewing and summariz- ment of Agriculture–Forest Service, Forest Health ing the literature and providing current information Technology Enterprise Team, Morgantown, West on biological control of the major invasive plants in Virginia, for providing funding for the preparation the Eastern United States. and printing of this publication. G. Keith Douce, David Moorhead, and Charles Additional copies of this publication can be or- Bargeron of the Bugwood Network, University of dered from the Bulletin Distribution Center, Uni- Georgia (Tifton, Ga.), managed and digitized the pho- versity of Massachusetts, Amherst, MA 01003, (413) tographs and illustrations used in this publication and 545-2717; or Mark Hoddle, Department of Entomol- produced the CD-ROM accompanying this book.
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												  Euphorbia Esula) Biocontrol Program Following Herbicide ApplicationUniversity of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln USGS Northern Prairie Wildlife Research Center US Geological Survey 2007 Short-term Disruption of a Leafy Spurge (Euphorbia esula) Biocontrol Program Following Herbicide Application Diane L. Larson USGS Northern Prairie Wildlife Research Center, [email protected] James B. Grace USGS National Wetlands Research Center, Lafayette, LA, [email protected] Paul A. Rabie University of Minnesota, St. Paul, [email protected] Paula Andersen Theodore Roosevelt National Park Follow this and additional works at: https://digitalcommons.unl.edu/usgsnpwrc Part of the Other International and Area Studies Commons Larson, Diane L.; Grace, James B.; Rabie, Paul A.; and Andersen, Paula, "Short-term Disruption of a Leafy Spurge (Euphorbia esula) Biocontrol Program Following Herbicide Application" (2007). USGS Northern Prairie Wildlife Research Center. 84. https://digitalcommons.unl.edu/usgsnpwrc/84 This Article is brought to you for free and open access by the US Geological Survey at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in USGS Northern Prairie Wildlife Research Center by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Biological Control 40 (2007) 1–8 www.elsevier.com/locate/ybcon Commentary Short-term disruption of a leafy spurge (Euphorbia esula) biocontrol program following herbicide application Diane L. Larson a,*, James B. Grace b, Paul A. Rabie c,1, Paula Andersen d,2 a USGS Northern Prairie Wildlife Research Center, Minnesota Field Station, 100 Ecology Building, 1987 Upper Buford Circle, St. Paul, MN 55108, USA b USGS National Wetlands Research Center, Lafayette, LA, USA c University of Minnesota, St.
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												  Mechanisms of Cabbage Seedpod Weevil ResistanceUniversity of Alberta Mechanisms of cabbage seedpod weevil, Ceutorhynchus obstrictus , resistance associated with novel germplasm derived from Sinapis alba x Brassica napus by James Allen Tansey A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Plant Science Department of Agricultural, Food and Nutritional Sciences ©James Allen Tansey Fall, 2009 Edmonton, Alberta Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission. University of Alberta Faculty of Graduate Studies and Research The undersigned certify that they have read, and recommend to the Faculty of Graduate Studies and Research for acceptance, a thesis entitled ‘Mechanisms of cabbage seedpod weevil, Ceutorhynchus obstrictus resistance associated with novel germplasm derived from Sinapis alba x Brassica napus ’ submitted by James A. Tansey in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Agricultural, Food and Nutritional Science. Examining Committee Dr. Lloyd M. Dosdall, Agricultural, Food and Nutritional Science Dr.
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												  Abstract IntroductionAbstract By nature, invasive exotic species are difficult to control. The more management tools that are available to weed managers, the greater the chance that a particular noxious weed will successfully be controlled. Arapahoe County has made the decision to explore the use of beneficial insects to complement herbicide applications and mechanical controls. Insects for leafy spurge have been released in locations throughout the County to help provide some control. Three different Apthona flea beetle species have been released as well as Oberea erythrocephala, a stem and root-boring beetle. To date the Aphthona flea beetles have proven to be the most effective for leafy spurge control and results on the effectiveness of Oberea erythrocephala are still inconclusive. Introduction Leafy spurge is a non-native plant originally from Europe that will invade riparian areas, rangelands, pastures, and waste areas. The main reason it is successful at invading these sites is its massive underground root system. It can grow to depths of 30 feet and can sprout new plants from root fragments as small as ½ inch long. Plants will vary in height from 1 to 3 feet and are pale green in color. All parts of the plant contain a white, milky latex sap that can inhibit the movement of some herbicides into the root system and is poisonous to both humans and animals. For the past five years Arapahoe County has been implementing biological controls on both public and private lands as a means of noxious weed control. In general biological controls, or beneficial insects, have proven largely to be successful.
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												  Background and General Information 2United States Department of National Program 304: Agriculture Agricultural Crop Protection and Research Service Quarantine National Program Staff August 2007 TABLE OF CONTENTS Background and General Information 2 Component I: Identification and Classification of Insects and Mites 5 Component II: Biology of Pests and Natural Enemies (Including Microbes) 8 Component III: Plant, Pest, and Natural Enemy Interactions and Ecology 17 Component IV: Postharvest, Pest Exclusion, and Quarantine Treatment 24 Component V: Pest Control Technologies 30 Component VI: Integrated Pest Management Systems and Areawide Suppression 41 Component VII: Weed Biology and Ecology 48 Component VIII: Chemical Control of Weeds 53 Component IX: Biological Control of Weeds 56 Component X: Weed Management Systems 64 APPENDIXES – Appendix 1: ARS National Program Assessment 70 Appendix 2: Documentation of NP 304 Accomplishments 73 NP 304 Accomplishment Report, 2001-2006 Page 2 BACKGROUND AND GENERAL INFORMATION THE AGRICULTURAL RESEARCH SERVICE The Agricultural Research Service (ARS) is the intramural research agency for the U.S. Department of Agriculture (USDA), and is one of four agencies that make up the Research, Education, and Economics mission area of the Department. ARS research comprises 21 National Programs and is conducted at 108 laboratories spread throughout the United States and overseas by over 2,200 full-time scientists within a total workforce of 8,000 ARS employees. The research in National Program 304, Crop Protection and Quarantine, is organized into 140 projects, conducted by 236 full-time scientists at 41 geographic locations. At $102.8 million, the fiscal year (FY) 2007 net research budget for National Program 304 represents almost 10 percent of ARS’s total FY 2007 net research budget of $1.12 billion.