Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells

Home , ABTB1, C12orf43, EIF5A2, EVI5L, HIST1H1B, HIST1H2AH

Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma cells

Xiaoli Wangb$, Yi Guoa$, Shu Yanga, Caihong Wangb, Xuping Fua, Jinling Wangb, Yumin Maoa,

Junsong Zhangb*, Yao Lia*

aState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University,

Shanghai 200433, P.R.China

bSchool of Applied Chemistry and Biological, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, P.R.China.

The Journal of Pharmacology and Experimental Therapeutics

Supplement Table S1. Genes up-regulated in all experimental groups

GeneID Genetic information Gene No. chromosome Ratio

RN7SK Homo sapiens RNA, 7SK, nuclear (RN7SK) on chromosome 6. NR_001445.1 6 320.633

HIST2H3C Homo sapiens histone 2, H3c (HIST2H3C), mRNA. NM_021059.2 48.67

BP873537 Sugano cDNA library, embryonal kidney Homo sapiens cDNA clone 47.045 HS.562219 HKR13896, mRNA sequence BP873537

HIST1H4B Homo sapiens histone 1, H4b (HIST1H4B), mRNA. NM_003544.2 6 42.321

BX109218 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone 42.115 HS.245367 IMAGp998C03690, mRNA sequence BX109218

TRQ1 Homo sapiens tRNA glutamine 1 (TRQ1) on chromosome 17. NR_000037.1 15 38.195

HIST1H2AH Homo sapiens histone 1, H2ah (HIST1H2AH), mRNA. NM_080596.1 6 33.628

HS.545589 Human small nuclear RNA U6atac, partial sequence U62823 32.42

LOC440686 Homo sapiens similar to histone H2B histone family (LOC440686), mRNA. NM_001025303.1 1 32.014

RNU12 Homo sapiens RNA, U12 small nuclear (RNU12) on chromosome X. NR_000041.1 X 31.693

HIST1H1B Homo sapiens histone 1, H1b (HIST1H1B), mRNA. NM_005322.2 6 27.632

TRK1 Homo sapiens tRNA lysine 1 (TRK1) on chromosome 17. NR_001449.1 11 27.546

RPPH1 Homo sapiens ribonuclease P RNA component H1 (RPPH1) on chromosome 14. NR_002312.1 14 23.993

HIST1H2BG Homo sapiens histone 1, H2bg (HIST1H2BG), mRNA. NM_003518.3 6 16.779

BX093900 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGp998M23189, 13.798 HS.443602 mRNA sequence BX093900

FOXD4L1 Homo sapiens forkhead box D4-like 1 (FOXD4L1), mRNA. NM_012184.3 2 13.36

HS.545112 Homo sapiens mRNA; cDNA DKFZp434F0472 (from clone DKFZp434F0472) AL137331 12.512

ws25d05.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE:2498217 3, mRNA 12.003 HS.197703 sequence AI989490

Homo sapiens inhibitor of DNA binding 2, dominant negative helix-loop-helix protein 9.983 ID2 (ID2), mRNA. NM_002166.4 2

tt27g09.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE:2242048 3, mRNA 9.793 HS.371006 sequence AI655567

TSPYL3 PREDICTED: Homo sapiens TSPY-like 3 (pseudogene) (TSPYL3), misc RNA. XR_001421.1 9.651

PREDICTED: Homo sapiens similar to Kinase suppressor of ras-1 (Kinase 9.336 LOC649456 suppressor of ras) (LOC649456), mRNA. XM_938534.1

PREDICTED: Homo sapiens similar to Bcl-2-associated transcription factor 1 (Btf), 8.578 LOC650759 transcript variant 1 (LOC650759), mRNA. XM_940798.1

BX110944 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGp998E24200, 7.462 HS.297929 mRNA sequence BX110944

ta96c03.x1 NCI_CGAP_Lu26 Homo sapiens cDNA clone IMAGE:2051908 3, mRNA 7.292 HS.436134 sequence AI349750

AGENCOURT_14535501 NIH_MGC_191 Homo sapiens cDNA clone 5.816 HS.543887 IMAGE:30415823 5, mRNA sequence CD640673

HIST1H4H Homo sapiens histone 1, H4h (HIST1H4H), mRNA. NM_003543.3 6 5.726

HS.550111 AV647906 GLC Homo sapiens cDNA clone GLCBDC04 3, mRNA sequence AV647906 5.265

EOMES Homo sapiens eomesodermin homolog (Xenopus laevis) (EOMES), mRNA. NM_005442.2 3 4.905

RNU15B Homo sapiens RNA, U15b small nucleolar (RNU15B) on chromosome 11. NR_000025.1 11 4.8 KRT6E Homo sapiens keratin 6E (KRT6E), mRNA. NM_173086.2 12 4.702

TMEM70 Homo sapiens transmembrane protein 70 (TMEM70), mRNA. NM_017866.3 8 4.627

BX113978 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone 4.607 HS.370110 IMAGp998N131206, mRNA sequence BX113978

POLR1B Homo sapiens polymerase (RNA) I polypeptide B, 128kDa (POLR1B), mRNA. NM_019014.3 2 4.474

C14ORF172 Homo sapiens chromosome 14 open reading frame 172 (C14orf172), mRNA. NM_152307.1 14 4.413

LOC648852 PREDICTED: Homo sapiens hypothetical protein LOC648852 (LOC648852), mRNA. XM_940430.1 4.323

JMJD4 Homo sapiens jumonji domain containing 4 (JMJD4), mRNA. NM_023007.1 1 4.294

Homo sapiens LanC lantibiotic synthetase component C-like 2 (bacterial) (LANCL2), 4.07 LANCL2 mRNA. NM_018697.3 7

NCOA5 Homo sapiens nuclear receptor coactivator 5 (NCOA5), mRNA. NM_020967.2 2 3.959

BY796152 Homo sapiens eye Homo sapiens cDNA clone 3.883 HS.544432 HEmEye2030G10_072.ab1 5, mRNA sequence BY796152

PUS3 Homo sapiens pseudouridylate synthase 3 (PUS3), mRNA. NM_031307.2 11 3.788

DDX51 Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 (DDX51), mRNA. NM_175066.2 12 3.724

PREDICTED: Homo sapiens similar to arginine/serine-rich splicing factor 6, transcript 3.614 LOC644422 variant 1 (LOC644422), mRNA. XM_930254.1 17

HUS1B Homo sapiens HUS1 checkpoint homolog b (S. pombe) (HUS1B), mRNA. NM_148959.2 6 3.588

C12ORF43 Homo sapiens chromosome 12 open reading frame 43 (C12orf43), mRNA. NM_022895.1 12 3.398

RWDD1 Homo sapiens RWD domain containing 1 (RWDD1), transcript variant 2, mRNA. NM_016104.2 6 3.387

PREDICTED: Homo sapiens similar to protein immuno-reactive with anti-PTH 3.351 LOC649841 polyclonal antibodies (LOC649841), mRNA. XM_938906.1

C7ORF36 Homo sapiens chromosome 7 open reading frame 36 (C7orf36), mRNA. NM_020192.1 7 3.342

Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), 3.301 MFHAS1 mRNA. NM_004225.1 8

Homo sapiens complement component 4 binding protein, beta (C4BPB), transcript 3.298 C4BPB variant 1, mRNA. NM_000716.3 1

MTG1 Homo sapiens mitochondrial GTPase 1 homolog (S. cerevisiae) (MTG1), mRNA. NM_138384.1 1 3.296

CGI-115 Homo sapiens CGI-115 protein (CGI-115), mRNA. NM_016052.2 1 3.264

ZNHIT2 Homo sapiens zinc finger, HIT type 2 (ZNHIT2), mRNA. NM_014205.2 11 3.204

GRWD1 Homo sapiens glutamate-rich WD repeat containing 1 (GRWD1), mRNA. NM_031485.2 19 3.184

LCMT2 Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA. NM_014793.3 15 3.147

GPATC3 Homo sapiens G patch domain containing 3 (GPATC3), mRNA. NM_022078.1 1 3.138

Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase 3.129 TRMU (TRMU), nuclear gene encoding mitochondrial protein, transcript variant 5, mRNA. NM_001008571.1 22

Homo sapiens piggyBac transposable element derived 2 (PGBD2), transcript variant 3.089 PGBD2 1, mRNA. NM_170725.2 1

KIAA0133 Homo sapiens KIAA0133 (KIAA0133), mRNA. NM_014777.1 1 3.064

LOC221710 PREDICTED: Homo sapiens hypothetical protein LOC221710 (LOC221710), mRNA. XM_927609.1 6 3.043

ALKBH8 Homo sapiens alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mRNA. NM_138775.1 11 3.036

FLJ20516 Homo sapiens timeless-interacting protein (FLJ20516), mRNA. NM_017858.1 15 3.027

MGC5242 Homo sapiens hypothetical protein MGC5242 (MGC5242), mRNA. NM_024033.1 7 3.018

Homo sapiens serpin peptidase inhibitor, clade B (ovalbumin), member 8 2.993 SERPINB8 (SERPINB8), transcript variant 3, mRNA. NM_001031848.1 18 C1ORF33 Homo sapiens chromosome 1 open reading frame 33 (C1orf33), mRNA. NM_016183.2 1 2.99

HSF2BP Homo sapiens heat shock transcription factor 2 binding protein (HSF2BP), mRNA. NM_007031.1 21 2.962

LOC644790 PREDICTED: Homo sapiens similar to ribosomal protein S15a (LOC644790), mRNA. XM_927887.1 1 2.91

P2RY11 Homo sapiens purinergic receptor P2Y, G-protein coupled, 11 (P2RY11), mRNA. NM_002566.4 19 2.9

KIAA0690 Homo sapiens KIAA0690 (KIAA0690), mRNA. NM_015179.2 1 2.888

TXNL1 Homo sapiens thioredoxin-like 1 (TXNL1), mRNA. NM_004786.1 18 2.879

DNAJC5 Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 5 (DNAJC5), mRNA. NM_025219.1 2 2.871

C17ORF32 Homo sapiens chromosome 17 open reading frame 32 (C17orf32), mRNA. NM_152464.1 17 2.85

BX111520 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGp998L15208, 2.829 HS.16515 mRNA sequence BX111520

DLEU1 PREDICTED: Homo sapiens deleted in lymphocytic leukemia, 1 (DLEU1), misc RNA. XR_001515.1 13 2.82

KLHL18 Homo sapiens kelch-like 18 (Drosophila) (KLHL18), mRNA. NM_025010.2 3 2.815

MSTO1 Homo sapiens misato homolog 1 (Drosophila) (MSTO1), mRNA. NM_018116.2 1 2.781

Homo sapiens ribonuclease P/MRP 38kDa subunit (RPP38), transcript variant 1, 2.778 RPP38 mRNA. NM_183005.1 1

PINX1 Homo sapiens PIN2-interacting protein 1 (PINX1), mRNA. NM_017884.3 8 2.759

IRX5 Homo sapiens iroquois homeobox protein 5 (IRX5), mRNA. NM_005853.4 16 2.758

PREDICTED: Homo sapiens similar to paraspeckle protein 1, transcript variant 2 2.73 LOC642395 (LOC642395), mRNA. XM_930623.1 13

C12ORF45 Homo sapiens chromosome 12 open reading frame 45 (C12orf45), mRNA. NM_152318.1 12 2.724

TMEM79 Homo sapiens transmembrane protein 79 (TMEM79), mRNA. NM_032323.1 1 2.721

Homo sapiens ankyrin repeat and SOCS box-containing 7 (ASB7), transcript variant 2.712 ASB7 1, mRNA. NM_024708.2 15

C6ORF157 Homo sapiens chromosome 6 open reading frame 157 (C6orf157), mRNA. NM_198920.1 6 2.702

HS.535360 Homo sapiens cDNA clone IMAGE:6250506 BC080616 2.698

Homo sapiens RRS1 ribosome biogenesis regulator homolog (S. cerevisiae) (RRS1), 2.695 RRS1 mRNA. NM_015169.3 8

MYL5 Homo sapiens myosin, light polypeptide 5, regulatory (MYL5), mRNA. NM_002477.1 4 2.688

ZC3H8 Homo sapiens zinc finger CCCH-type containing 8 (ZC3H8), mRNA. NM_032494.1 2 2.681

ANXA3 Homo sapiens annexin A3 (ANXA3), mRNA. NM_005139.1 4 2.672

C1ORF163 Homo sapiens chromosome 1 open reading frame 163 (C1orf163), mRNA. NM_023077.1 1 2.667

ZNF207 Homo sapiens zinc finger protein 207 (ZNF207), transcript variant 1, mRNA. NM_003457.2 17 2.667

ZNF232 Homo sapiens zinc finger protein 232 (ZNF232), mRNA. NM_014519.2 17 2.667

FLJ20309 Homo sapiens hypothetical protein FLJ20309 (FLJ20309), mRNA. NM_017759.2 2 2.643

EIF5A2 Homo sapiens eukaryotic translation initiation factor 5A2 (EIF5A2), mRNA. NM_020390.5 3 2.631

Homo sapiens M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) 2.599 MPHOSPH10 (MPHOSPH10), mRNA. NM_005791.1 2

REP15 Homo sapiens Rab15 effector protein (REP15), mRNA. NM_001029874.1 12 2.599

C12ORF5 Homo sapiens chromosome 12 open reading frame 5 (C12orf5), mRNA. NM_020375.1 12 2.591

HYAL3 Homo sapiens hyaluronoglucosaminidase 3 (HYAL3), mRNA. NM_003549.2 3 2.572

Homo sapiens MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP), 2.57 MKI67IP mRNA. NM_032390.3 2

Homo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit 2.567 PRKAB1 (PRKAB1), mRNA. NM_006253.4 12 602706795F1 NIH_MGC_43 Homo sapiens cDNA clone IMAGE:4843583 5, mRNA 2.557 HS.404514 sequence BG749175

Homo sapiens UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast) 2.553 UTP14A (UTP14A), mRNA. NM_006649.2 X

TUBB4Q Homo sapiens tubulin, beta polypeptide 4, member Q (TUBB4Q), mRNA. NM_020040.3 4 2.535

GPATC4 Homo sapiens G patch domain containing 4 (GPATC4), transcript variant 2, mRNA. NM_182679.1 1 2.531

Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, 2.528 EIF5 mRNA. NM_001969.3 14

WDR73 Homo sapiens WD repeat domain 73 (WDR73), mRNA. NM_032856.1 15 2.52

C1ORF73 Homo sapiens chromosome 1 open reading frame 73 (C1orf73), mRNA. NM_015434.1 1 2.505

Homo sapiens RNA pseudouridylate synthase domain containing 1 (RPUSD1), 2.502 RPUSD1 mRNA. NM_058192.2 16

NXF1 Homo sapiens nuclear RNA export factor 1 (NXF1), mRNA. NM_006362.3 11 2.494

Homo sapiens LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae) 2.49 LSM1 (LSM1), mRNA. NM_014462.1 8

PAK1IP1 Homo sapiens PAK1 interacting protein 1 (PAK1IP1), mRNA. NM_017906.2 6 2.471

PGBD1 Homo sapiens piggyBac transposable element derived 1 (PGBD1), mRNA. NM_032507.2 6 2.465

FGFR1OP Homo sapiens FGFR1 oncogene partner (FGFR1OP), transcript variant 1, mRNA. NM_007045.2 6 2.459

SURF2 Homo sapiens surfeit 2 (SURF2), mRNA. NM_017503.2 9 2.456

NTS Homo sapiens neurotensin (NTS), mRNA. NM_006183.3 12 2.449

CRY1 Homo sapiens cryptochrome 1 (photolyase-like) (CRY1), mRNA. NM_004075.2 12 2.445

KLHL21 Homo sapiens kelch-like 21 (Drosophila) (KLHL21), mRNA. NM_014851.2 1 2.439

Homo sapiens mitochondrial ribosomal protein L1 (MRPL1), nuclear gene encoding 2.427 MRPL1 mitochondrial protein, mRNA. NM_020236.2 4

Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, 2.415 BRF2 BRF1-like (BRF2), mRNA. NM_018310.2 8

PREDICTED: Homo sapiens similar to zinc finger and BTB domain containing 8 2.414 LOC646900 opposite strand (LOC646900), mRNA. XM_929862.1 2

WDR77 Homo sapiens WD repeat domain 77 (WDR77), mRNA. NM_024102.2 1 2.402

CIRBP Homo sapiens cold inducible RNA binding protein (CIRBP), mRNA. NM_001280.1 19 2.372

Homo sapiens polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa 2.371 POLR3F (POLR3F), mRNA. NM_006466.2 2

PWP2H Homo sapiens PWP2 periodic tryptophan protein homolog (yeast) (PWP2H), mRNA. NM_005049.2 21 2.365

RCL1 Homo sapiens RNA terminal phosphate cyclase-like 1 (RCL1), mRNA. NM_005772.2 9 2.365

DNAJC3 Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 3 (DNAJC3), mRNA. NM_006260.2 13 2.358

Homo sapiens polymerase (RNA) III (DNA directed) polypeptide C (62kD) (POLR3C), 2.356 POLR3C mRNA. NM_006468.5 1

TXNL4B Homo sapiens thioredoxin-like 4B (TXNL4B), mRNA. NM_017853.1 16 2.356

C14ORF169 Homo sapiens chromosome 14 open reading frame 169 (C14orf169), mRNA. NM_024644.1 14 2.352

FLJ20551 Homo sapiens hypothetical protein FLJ20551 (FLJ20551), mRNA. NM_017875.1 3 2.351

C9ORF112 Homo sapiens chromosome 9 open reading frame 112 (C9orf112), mRNA. NM_138778.1 9 2.348

Homo sapiens upstream binding transcription factor, RNA polymerase I (UBTF), 2.346 UBTF mRNA. NM_014233.1 17

WDR74 Homo sapiens WD repeat domain 74 (WDR74), mRNA. NM_018093.1 11 2.345 ZSCAN5 Homo sapiens zinc finger and SCAN domain containing 5 (ZSCAN5), mRNA. NM_024303.1 19 2.309

FLJ10803 Homo sapiens hypothetical protein FLJ10803 (FLJ10803), mRNA. NM_018224.1 7 2.293

C1ORF82 Homo sapiens chromosome 1 open reading frame 82 (C1orf82), mRNA. NM_024813.1 1 2.219

XPO5 Homo sapiens exportin 5 (XPO5), mRNA. NM_020750.1 6 2.205

LOC647769 PREDICTED: Homo sapiens hypothetical protein LOC647769 (LOC647769), mRNA. XM_943009.1 2.197

SUPV3L1 Homo sapiens suppressor of var1, 3-like 1 (S. cerevisiae) (SUPV3L1), mRNA. NM_003171.2 1 2.191

Homo sapiens Tar (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 1, 2.133 TARBP2 mRNA. NM_134323.1 12

Table S2. Genes down-regulated in all experimental groups

GeneID Genetic information Gene No. chromosome Ratio

HS.181245 Homo sapiens mRNA; cDNA DKFZp686B15184 (from clone DKFZp686B15184) BX640973 0.028

HCG27 Homo sapiens HLA complex group 27 (HCG27), mRNA. NM_181717.1 6 0.039

AL528570 Homo sapiens NEUROBLASTOMA COT 50-NORMALIZED Homo 0.042 HS.561735 sapiens cDNA clone CS0DD001YM19 5-PRIME, mRNA sequence AL528570

UI-E-EJ0-aiq-m-17-0-UI.r1 UI-E-EJ0 Homo sapiens cDNA clone 0.045 HS.133261 UI-E-EJ0-aiq-m-17-0-UI 5, mRNA sequence BM723547

Homo sapiens solute carrier family 2 (facilitated glucose transporter), member 3 0.049 SLC2A3 (SLC2A3), mRNA. NM_006931.1 12

HS.170035 Homo sapiens cDNA FLJ35432 fis, clone SMINT2002311 AK092751 0.051

FLJ36779 Homo sapiens hypothetical protein FLJ36779 (FLJ36779), mRNA. NM_152571.2 9 0.054

AGENCOURT_13565173 NIH_MGC_184 Homo sapiens cDNA clone 0.056 HS.48729 IMAGE:30326407 5, mRNA sequence CB984746

C17ORF55 Homo sapiens chromosome 17 open reading frame 55 (C17orf55), mRNA. NM_178519.2 17 0.057

PREDICTED: Homo sapiens chromosome 13 open reading frame 25 (C13orf25), 0.059 C13ORF25 mRNA. XM_931068.1 13

C10ORF10 Homo sapiens chromosome 10 open reading frame 10 (C10orf10), mRNA. NM_007021.2 1 0.062

FLJ22639 Homo sapiens hypothetical protein FLJ22639 (FLJ22639), mRNA. NM_024796.1 1 0.07

NFE2 Homo sapiens nuclear factor (erythroid-derived 2), 45kDa (NFE2), mRNA. NM_006163.1 12 0.071

ie62h02.y5 Melton Normalized Human Islet 4 N4-HIS 1 Homo sapiens cDNA clone 0.081 HS.524705 IMAGE:5671539 5, mRNA sequence CK903656

PREDICTED: Homo sapiens hypothetical protein LOC649639 (LOC649639), 0.088 LOC649639 mRNA. XM_943818.1

HS.131041 AV691356 GKC Homo sapiens cDNA clone GKCCBC07 5, mRNA sequence AV691356 0.091

HS.372654 Homo sapiens cDNA: FLJ23004 fis, clone LNG00391 AK026657 0.097

LOX Homo sapiens lysyl oxidase (LOX), mRNA. NM_002317.3 5 0.097

TJP3 Homo sapiens tight junction protein 3 (zona occludens 3) (TJP3), mRNA. NM_014428.1 19 0.098

FLJ45244 Homo sapiens FLJ45244 protein (FLJ45244), mRNA. NM_207443.1 14 0.099

PREDICTED: Homo sapiens hypothetical locus LOC338799, transcript variant 3 0.101 LOC338799 (LOC338799), misc RNA. XR_000910.1 12

FAM113B Homo sapiens family with sequence similarity 113, member B (FAM113B), mRNA. NM_138371.1 12 0.104

ACPP Homo sapiens acid phosphatase, prostate (ACPP), mRNA. NM_001099.2 3 0.105

602617110F1 NIH_MGC_79 Homo sapiens cDNA clone IMAGE:4730811 5, mRNA 0.109 HS.445843 sequence BG621527

HS.143408 Homo sapiens cDNA clone IMAGE:30332316 BC067908 0.113

FLJ42957 Homo sapiens FLJ42957 protein (FLJ42957), mRNA. NM_207436.1 12 0.115

GJA12 Homo sapiens gap junction protein, alpha 12, 47kDa (GJA12), mRNA. NM_020435.2 1 0.116

HS.231861 Homo sapiens cDNA FLJ13474 fis, clone PLACE1003593 AK023536 0.117

HS.120058 Homo sapiens cDNA FLJ34446 fis, clone HLUNG2002050 AK091765 0.118 PRSS8 Homo sapiens protease, serine, 8 (prostasin) (PRSS8), mRNA. NM_002773.2 16 0.119

PREDICTED: Homo sapiens similar to NADH dehydrogenase (ubiquinone) 1 beta 0.126 LOC653432 subcomplex, 4, 15kDa, transcript variant 1 (LOC653432), mRNA. XM_928990.1 3

Homo sapiens Ral GEF with PH domain and SH3 binding motif 1 (RALGPS1), 0.126 RALGPS1 mRNA. NM_014636.1 9

HS.71947 Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 994183 AJ420536 0.127

HS.267120 Homo sapiens mRNA; cDNA DKFZp666J249 (from clone DKFZp666J249) AL833054 0.128

RIMS3 Homo sapiens regulating synaptic membrane exocytosis 3 (RIMS3), mRNA. NM_014747.2 1 0.131

Homo sapiens oxysterol binding protein-like 7 (OSBPL7), transcript variant 1, 0.135 OSBPL7 mRNA. NM_145798.2 17

VSIG8 Homo sapiens V-set and immunoglobulin domain containing 8 (VSIG8), mRNA. NM_001013661.1 1 0.136

DA728582 NT2RM2 Homo sapiens cDNA clone NT2RM2002174 5, mRNA 0.141 HS.574590 sequence DA728582

F2R Homo sapiens coagulation factor II (thrombin) receptor (F2R), mRNA. NM_001992.2 5 0.142

SOX12 Homo sapiens SRY (sex determining region Y)-box 12 (SOX12), mRNA. NM_006943.2 2 0.147

HS.408455 Homo sapiens mRNA; cDNA DKFZp686J1595 (from clone DKFZp686J1595) BX538057 0.148

KLHL24 Homo sapiens kelch-like 24 (Drosophila) (KLHL24), mRNA. NM_017644.3 3 0.148

GDF15 Homo sapiens growth differentiation factor 15 (GDF15), mRNA. NM_004864.1 19 0.151

MGC16121 PREDICTED: Homo sapiens hypothetical protein MGC16121 (MGC16121), mRNA. XM_927600.1 X 0.151

PREDICTED: Homo sapiens hypothetical protein LOC646123 (LOC646123), 0.152 LOC646123 mRNA. XM_933424.1 22

Homo sapiens Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), 0.162 RAPGEF3 mRNA. NM_006105.3 12

PREDICTED: Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 0.165 PPP1R3E 3E (PPP1R3E), mRNA. XM_940069.1

ANKRD37 Homo sapiens ankyrin repeat domain 37 (ANKRD37), mRNA. NM_181726.1 4 0.167

HS.553301 AV737317 CB Homo sapiens cDNA clone CBCAQH03 5, mRNA sequence AV737317 0.169

AGENCOURT_8109349 Lupski_sympathetic_trunk Homo sapiens cDNA clone 0.171 HS.572030 IMAGE:6189406 5, mRNA sequence BQ717127

PLD1 Homo sapiens phospholipase D1, phosphatidylcholine-specific (PLD1), mRNA. NM_002662.2 3 0.172

HS.365312 PREDICTED: Homo sapiens hypothetical LOC389250 (LOC389250), mRNA XM_374101 0.175

STC1 Homo sapiens stanniocalcin 1 (STC1), mRNA. NM_003155.2 8 0.186

ANGPTL4 Homo sapiens angiopoietin-like 4 (ANGPTL4), transcript variant 1, mRNA. NM_139314.1 19 0.199

GATS Homo sapiens opposite strand transcription unit to STAG3 (GATS), mRNA. NM_178831.4 7 0.199

HS.123119 Homo sapiens cDNA FLJ37828 fis, clone BRSSN2006575 AK095147 0.203

Homo sapiens integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) 0.203 ITGA2 (ITGA2), mRNA. NM_002203.2 5

ZFP36 Homo sapiens zinc finger protein 36, C3H type, homolog (mouse) (ZFP36), mRNA. NM_003407.1 19 0.203

ATP8B3 Homo sapiens ATPase, Class I, type 8B, member 3 (ATP8B3), mRNA. NM_138813.2 19 0.205

ANKRD22 Homo sapiens ankyrin repeat domain 22 (ANKRD22), mRNA. NM_144590.1 1 0.208

Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), nuclear gene 0.208 MRPS25 encoding mitochondrial protein, mRNA. NM_022497.3 3

DA160026 BRAMY2 Homo sapiens cDNA clone BRAMY2019928 5, mRNA 0.209 HS.575222 sequence DA160026 DIDO1 Homo sapiens death inducer-obliterator 1 (DIDO1), transcript variant 3, mRNA. NM_080797.2 2 0.213

DKFZP686I152 0.213 17 Homo sapiens hypothetical protein DKFZp686I15217 (DKFZp686I15217), mRNA. NM_207495.2 6

HS.356079 Homo sapiens cDNA FLJ42149 fis, clone THYMU1000692 AK124143 0.214

TC125227 Human breast cancer tissue, large insert, pCMV expression library

Homo sapiens cDNA clone TC125227 5 similar to Homo sapiens LOC399828 0.218

HS.568741 (LOC387724), mRNA sequence DN997246

Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (ST3GAL5), 0.219 ST3GAL5 mRNA. NM_003896.2 2

NGEF Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), mRNA. NM_019850.1 2 0.226

Homo sapiens prostaglandin-endoperoxide synthase 1 (prostaglandin G/H 0.228 PTGS1 synthase and cyclooxygenase) (PTGS1), transcript variant 2, mRNA. NM_080591.1 9

ANTXR2 Homo sapiens anthrax toxin receptor 2 (ANTXR2), mRNA. NM_058172.3 4 0.233

Homo sapiens solute carrier family 4, sodium bicarbonate cotransporter, member 7 0.234 SLC4A7 (SLC4A7), mRNA. NM_003615.2 3

HIST2H2BE Homo sapiens histone 2, H2be (HIST2H2BE), mRNA. NM_003528.2 1 0.236

NDRG1 Homo sapiens N-myc downstream regulated gene 1 (NDRG1), mRNA. NM_006096.2 8 0.24

KIAA1904 Homo sapiens KIAA1904 protein (KIAA1904), mRNA. NM_052906.1 22 0.244

Homo sapiens opsin 3 (encephalopsin, panopsin) (OPN3), transcript variant 3, 0.244 OPN3 mRNA. NM_001030012.1 1

CLIC3 Homo sapiens chloride intracellular channel 3 (CLIC3), mRNA. NM_004669.2 9 0.246

CNTNAP1 Homo sapiens contactin associated protein 1 (CNTNAP1), mRNA. NM_003632.1 17 0.247

PLAT Homo sapiens plasminogen activator, tissue (PLAT), transcript variant 1, mRNA. NM_000930.2 8 0.251

Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 3 0.252 ABCC3 (ABCC3), transcript variant MRP3A, mRNA. NM_020037.1 17

ZNF467 Homo sapiens zinc finger protein 467 (ZNF467), mRNA. NM_207336.1 7 0.252

DENND3 Homo sapiens DENN/MADD domain containing 3 (DENND3), mRNA. NM_014957.2 8 0.253

KIAA1539 Homo sapiens KIAA1539 (KIAA1539), mRNA. NM_025182.2 9 0.254

RHBDF1 Homo sapiens rhomboid 5 homolog 1 (Drosophila) (RHBDF1), mRNA. NM_022450.2 16 0.255

LMO7 Homo sapiens LIM domain 7 (LMO7), mRNA. NM_005358.3 13 0.256

IL1B Homo sapiens interleukin 1, beta (IL1B), mRNA. NM_000576.2 2 0.258

LRG1 Homo sapiens leucine-rich alpha-2-glycoprotein 1 (LRG1), mRNA. NM_052972.2 19 0.258

RAB26 Homo sapiens RAB26, member RAS oncogene family (RAB26), mRNA. NM_014353.4 16 0.259

GCNT3 Homo sapiens glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), mRNA. NM_004751.1 15 0.26

EVI5L Homo sapiens ecotropic viral integration site 5-like (EVI5L), mRNA. NM_145245.2 19 0.263

PREDICTED: Homo sapiens hypothetical protein LOC643272 (LOC643272), 0.263 LOC643272 mRNA. XM_926633.1 3

Homo sapiens alpha 1,4-galactosyltransferase (globotriaosylceramide synthase) 0.266 A4GALT (A4GALT), mRNA. NM_017436.4 22

CYBRD1 Homo sapiens cytochrome b reductase 1 (CYBRD1), mRNA. NM_024843.2 2 0.269

TMEM118 Homo sapiens transmembrane protein 118 (TMEM118), mRNA. NM_032814.1 12 0.27

ANG Homo sapiens angiogenin, ribonuclease, RNase A family, 5 (ANG), mRNA. NM_001145.2 14 0.272

Homo sapiens Cbp/p300-interacting transactivator, with Glu/Asp-rich 0.273 CITED4 carboxy-terminal domain, 4 (CITED4), mRNA. NM_133467.2 1 LOC201895 Homo sapiens hypothetical protein LOC201895 (LOC201895), mRNA. NM_174921.1 4 0.274

Homo sapiens solute carrier family 20 (phosphate transporter), member 1 0.276 SLC20A1 (SLC20A1), mRNA. NM_005415.3 2

Homo sapiens vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), 0.278 VAMP1 transcript variant 2, mRNA. NM_199245.1 12

Homo sapiens MOB1, Mps One Binder kinase activator-like 2A (yeast) 0.28 MOBKL2A (MOBKL2A), mRNA. NM_130807.2 19

CXORF12 Homo sapiens chromosome X open reading frame 12 (CXorf12), mRNA. NM_003492.1 X 0.281

FBXL2 Homo sapiens F-box and leucine-rich repeat protein 2 (FBXL2), mRNA. NM_012157.2 3 0.282

ELLS1 Homo sapiens hypothetical protein Ells1 (Ells1), mRNA. NM_152793.1 7 0.284

ACTA2 Homo sapiens actin, alpha 2, smooth muscle, aorta (ACTA2), mRNA. NM_001613.1 1 0.285

CBX7 Homo sapiens chromobox homolog 7 (CBX7), mRNA. NM_175709.2 22 0.287

MYO18A Homo sapiens myosin XVIIIA (MYO18A), transcript variant 2, mRNA. NM_203318.1 17 0.287

Homo sapiens development and differentiation enhancing factor-like 1 (DDEFL1), 0.29 DDEFL1 mRNA. NM_017707.2 1

Homo sapiens solute carrier family 27 (fatty acid transporter), member 1 0.29 SLC27A1 (SLC27A1), mRNA. NM_198580.1 19

NLGN2 Homo sapiens neuroligin 2 (NLGN2), mRNA. NM_020795.2 17 0.292

PREDICTED: Homo sapiens hypothetical protein LOC652226 (LOC652226), 0.298 LOC652226 mRNA. XM_945303.1

KLF9 Homo sapiens Kruppel-like factor 9 (KLF9), mRNA. NM_001206.2 9 0.3

ATXN1 Homo sapiens ataxin 1 (ATXN1), mRNA. NM_000332.2 6 0.302

CLDN7 Homo sapiens claudin 7 (CLDN7), mRNA. NM_001307.3 17 0.303

FLJ40142 Homo sapiens FLJ40142 protein (FLJ40142), mRNA. NM_207435.1 12 0.303

GSDML Homo sapiens gasdermin-like (GSDML), mRNA. NM_018530.1 17 0.305

602507996F1 NIH_MGC_79 Homo sapiens cDNA clone IMAGE:4605190 5, mRNA 0.307 HS.88156 sequence BG434785

HIP1 Homo sapiens huntingtin interacting protein 1 (HIP1), mRNA. NM_005338.4 7 0.308

MXD1 Homo sapiens MAX dimerization protein 1 (MXD1), mRNA. NM_002357.2 2 0.308

Homo sapiens low density lipoprotein receptor (familial hypercholesterolemia) 0.311 LDLR (LDLR), mRNA. NM_000527.2 19

Homo sapiens solute carrier family 17 (anion/sugar transporter), member 5 0.316 SLC17A5 (SLC17A5), mRNA. NM_012434.3 6

HS.19339 Homo sapiens cDNA clone IMAGE:5263177 BC035116 0.32

HS.294103 Homo sapiens mRNA; cDNA DKFZp686P23119 (from clone DKFZp686P23119) BX537605 0.32

Homo sapiens cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase 0.321 CCBL1 K, kyneurenine aminotransferase) (CCBL1), mRNA. NM_004059.3 9

INSIG1 Homo sapiens insulin induced gene 1 (INSIG1), transcript variant 1, mRNA. NM_005542.3 7 0.321

GLCE Homo sapiens glucuronyl C5-epimerase (GLCE), mRNA. NM_015554.1 15 0.324

Homo sapiens guanine nucleotide binding protein (G protein), alpha 15 (Gq class) 0.326 GNA15 (GNA15), mRNA. NM_002068.1 19

PIM1 Homo sapiens pim-1 oncogene (PIM1), mRNA. NM_002648.2 6 0.326

Homo sapiens CD55 antigen, decay accelerating factor for complement (Cromer 0.327 CD55 blood group) (CD55), mRNA. NM_000574.2 1 SLC44A2 Homo sapiens solute carrier family 44, member 2 (SLC44A2), mRNA. NM_020428.2 19 0.327

GBP2 Homo sapiens guanylate binding protein 2, interferon-inducible (GBP2), mRNA. NM_004120.3 1 0.328

MGC24665 Homo sapiens hypothetical protein MGC24665 (MGC24665), mRNA. NM_152308.1 16 0.33

HS.478682 Homo sapiens cDNA FLJ31090 fis, clone IMR321000102 AK055652 0.333

MGC33839 Homo sapiens hypothetical protein MGC33839 (MGC33839), mRNA. NM_152353.1 19 0.333

Homo sapiens butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 1, 0.334 BTN3A3 mRNA. NM_006994.3 6

EDN2 Homo sapiens endothelin 2 (EDN2), mRNA. NM_001956.2 1 0.338

Homo sapiens sparc/osteonectin, cwcv and kazal-like domains proteoglycan 0.339 SPOCK2 (testican) 2 (SPOCK2), mRNA. NM_014767.1 1

Homo sapiens ankyrin repeat and BTB (POZ) domain containing 1 (ABTB1), 0.34 ABTB1 transcript variant 2, mRNA. NM_172027.1 3

ARL14 Homo sapiens ADP-ribosylation factor-like 14 (ARL14), mRNA. NM_025047.1 3 0.341

BAIAP2L2 Homo sapiens BAI1-associated protein 2-like 2 (BAIAP2L2), mRNA. NM_025045.3 22 0.342

PREDICTED: Homo sapiens ring finger protein 5 pseudogene 1 (RNF5P1), misc 0.342 RNF5P1 RNA. XR_000528.1 8

SLC38A2 Homo sapiens solute carrier family 38, member 2 (SLC38A2), mRNA. NM_018976.3 12 0.343

TMEM44 Homo sapiens transmembrane protein 44 (TMEM44), transcript variant 1, mRNA. NM_138399.3 3 0.343

Homo sapiens integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), 0.344 ITGA5 mRNA. NM_002205.2 12

Homo sapiens MADS box transcription enhancer factor 2, polypeptide D (myocyte 0.345 MEF2D enhancer factor 2D) (MEF2D), mRNA. NM_005920.2 1

ZNF488 Homo sapiens zinc finger protein 488 (ZNF488), mRNA. NM_153034.2 1 0.345

Homo sapiens microtubule-associated protein, RP/EB family, member 3 0.347 MAPRE3 (MAPRE3), mRNA. NM_012326.2 2

Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), 0.348 PFKFB4 mRNA. NM_004567.2 3

7l60c12.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone 0.349 HS.130313 IMAGE:3525886 3, mRNA sequence BF109232

HS.443490 Homo sapiens cDNA FLJ32174 fis, clone PLACE6001064 AK056736 0.349

LOC653626 PREDICTED: Homo sapiens similar to ARG99 protein (LOC653626), mRNA. XM_928461.1 12 0.349

CCPG1 Homo sapiens cell cycle progression 1 (CCPG1), transcript variant 1, mRNA. NM_004748.3 15 0.35

MGC50722 Homo sapiens hypothetical MGC50722 (MGC50722), mRNA. NM_203348.1 0.35

RBM33 Homo sapiens RNA binding motif protein 33 (RBM33), mRNA. NM_001008408.1 7 0.351

C17ORF69 Homo sapiens chromosome 17 open reading frame 69 (C17orf69), mRNA. NM_152466.1 17 0.353

ENO2 Homo sapiens enolase 2 (gamma, neuronal) (ENO2), mRNA. NM_001975.2 12 0.353

APEG1 Homo sapiens aortic preferentially expressed gene 1 (APEG1), mRNA. NM_005876.3 2 0.354

C9ORF127 Homo sapiens chromosome 9 open reading frame 127 (C9orf127), mRNA. NM_016446.2 9 0.357

HCP5 Homo sapiens HLA complex P5 (HCP5), mRNA. NM_006674.2 6 0.357

Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 3 (ST3GAL3), 0.357 ST3GAL3 transcript variant 10, mRNA. NM_006279.2 1

GFPT2 Homo sapiens glutamine-fructose-6-phosphate transaminase 2 (GFPT2), mRNA. NM_005110.1 5 0.358

KIAA1815 Homo sapiens KIAA1815 (KIAA1815), mRNA. NM_024896.2 9 0.358

TMEM49 Homo sapiens transmembrane protein 49 (TMEM49), mRNA. NM_030938.2 17 0.36 GPR3 Homo sapiens G protein-coupled receptor 3 (GPR3), mRNA. NM_005281.2 1 0.362

Homo sapiens solute carrier family 6, member 15 (SLC6A15), transcript variant 1, 0.362 SLC6A15 mRNA. NM_182767.3 12

Homo sapiens CD47 antigen (Rh-related antigen, integrin-associated signal 0.363 CD47 transducer) (CD47), transcript variant 4, mRNA. NM_001025080.1 3

RP2 Homo sapiens retinitis pigmentosa 2 (X-linked recessive) (RP2), mRNA. NM_006915.1 X 0.363

SYTL4 Homo sapiens synaptotagmin-like 4 (granuphilin-a) (SYTL4), mRNA. NM_080737.1 X 0.363

Homo sapiens sparc/osteonectin, cwcv and kazal-like domains proteoglycan 0.364 SPOCK (testican) (SPOCK), mRNA. NM_004598.2 5

HS.535028 Homo sapiens cDNA: FLJ22720 fis, clone HSI14320 AK026373 0.366

Homo sapiens Williams Beuren syndrome chromosome region 27 (WBSCR27), 0.366 WBSCR27 mRNA. NM_152559.2 7

Homo sapiens protein phosphatase 2C, magnesium-dependent, catalytic subunit 0.368 PPM2C (PPM2C), nuclear gene encoding mitochondrial protein, mRNA. NM_018444.2 8

HS.91389 Homo sapiens cDNA clone IMAGE:3079901 BC090942 0.369

SCNN1A Homo sapiens sodium channel, nonvoltage-gated 1 alpha (SCNN1A), mRNA. NM_001038.4 12 0.37

Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 12 (CYP4F12), 0.371 CYP4F12 mRNA. NM_023944.1 19

LRRC58 PREDICTED: Homo sapiens leucine rich repeat containing 58 (LRRC58), mRNA. XM_938862.1 0.371

MT1F Homo sapiens metallothionein 1F (functional) (MT1F), mRNA. NM_005949.1 16 0.371

HRIHFB2122 Homo sapiens Tara-like protein (HRIHFB2122), transcript variant 1, mRNA. NM_007032.3 22 0.372

Homo sapiens oxysterol binding protein-like 5 (OSBPL5), transcript variant 1, 0.372 OSBPL5 mRNA. NM_020896.2 11

PALM Homo sapiens paralemmin (PALM), mRNA. NM_002579.1 19 0.373

DDIT4 Homo sapiens DNA-damage-inducible transcript 4 (DDIT4), mRNA. NM_019058.2 1 0.374

Homo sapiens basic helix-loop-helix domain containing, class B, 2 (BHLHB2), 0.375 BHLHB2 mRNA. NM_003670.1 3

MGC59937 Homo sapiens Similar to RIKEN cDNA 2310002J15 gene (MGC59937), mRNA. NM_199001.1 9 0.376

MMD Homo sapiens monocyte to macrophage differentiation-associated (MMD), mRNA. NM_012329.2 17 0.376

GRAMD1A Homo sapiens GRAM domain containing 1A (GRAMD1A), mRNA. NM_020895.2 19 0.377

Homo sapiens acyl-CoA synthetase long-chain family member 3 (ACSL3), 0.379 ACSL3 transcript variant 1, mRNA. NM_004457.3 2

HS.154336 Homo sapiens clone 24583 mRNA sequence AF070595 0.379

Homo sapiens glioma tumor suppressor candidate region gene 1 (GLTSCR1), 0.381 GLTSCR1 mRNA. NM_015711.1 19

INSIG2 Homo sapiens insulin induced gene 2 (INSIG2), mRNA. NM_016133.2 2 0.383

Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, 0.383 MLLT7 Drosophila); translocated to, 7 (MLLT7), mRNA. NM_005938.1 X

Homo sapiens transient receptor potential cation channel, subfamily M, member 4 0.386 TRPM4 (TRPM4), mRNA. NM_017636.2 19

HEG1 PREDICTED: Homo sapiens HEG homolog 1 (zebrafish) (HEG1), mRNA. XM_087386.8 3 0.387

Homo sapiens BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L), 0.39 BNIP3L mRNA. NM_004331.2 8

LKAP Homo sapiens limkain b1 (LKAP), mRNA. NM_014647.1 16 0.39 IGF2R Homo sapiens insulin-like growth factor 2 receptor (IGF2R), mRNA. NM_000876.1 6 0.391

HS1BP3 Homo sapiens HS1-binding protein 3 (HS1BP3), mRNA. NM_022460.3 2 0.392

C3ORF40 Homo sapiens chromosome 3 open reading frame 40 (C3orf40), mRNA. NM_144635.3 3 0.395

SNX26 Homo sapiens sorting nexin 26 (SNX26), mRNA. NM_052948.2 19 0.395

HBP1 Homo sapiens HMG-box transcription factor 1 (HBP1), mRNA. NM_012257.3 7 0.397

KIAA0323 Homo sapiens KIAA0323 (KIAA0323), mRNA. NM_015299.1 14 0.397

LMTK3 PREDICTED: Homo sapiens lemur tyrosine kinase 3 (LMTK3), mRNA. XM_936372.1 0.397

HS.335413 Homo sapiens, clone IMAGE:5241654, mRNA BC038245 0.398

CD109 Homo sapiens CD109 antigen (Gov platelet alloantigens) (CD109), mRNA. NM_133493.1 6 0.399

Homo sapiens myeloma overexpressed gene (in a subset of t(11;14) positive 0.406 MYEOV multiple myelomas) (MYEOV), mRNA. NM_138768.2 11

DIP Homo sapiens death-inducing-protein (DIP), mRNA. NM_015124.2 22 0.407

ZNF512 Homo sapiens zinc finger protein 512 (ZNF512), mRNA. NM_032434.2 2 0.408

Homo sapiens membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript 0.413 2-Mar variant 2, mRNA. NM_001005415.1 19

CPD Homo sapiens carboxypeptidase D (CPD), mRNA. NM_001304.3 17 0.413

C1ORF66 Homo sapiens chromosome 1 open reading frame 66 (C1orf66), mRNA. NM_015997.2 1 0.414

EVPL Homo sapiens envoplakin (EVPL), mRNA. NM_001988.1 17 0.414

SOX13 Homo sapiens SRY (sex determining region Y)-box 13 (SOX13), mRNA. NM_005686.2 1 0.416

C1ORF106 Homo sapiens chromosome 1 open reading frame 106 (C1orf106), mRNA. NM_018265.1 1 0.417

C10ORF33 Homo sapiens chromosome 10 open reading frame 33 (C10orf33), mRNA. NM_032709.1 1 0.418

RHOU Homo sapiens ras homolog gene family, member U (RHOU), mRNA. NM_021205.4 1 0.419

C14ORF147 Homo sapiens chromosome 14 open reading frame 147 (C14orf147), mRNA. NM_138288.2 14 0.42

F2RL1 Homo sapiens coagulation factor II (thrombin) receptor-like 1 (F2RL1), mRNA. NM_005242.3 5 0.422

Homo sapiens cysteine-rich with EGF-like domains 1 (CRELD1), transcript variant 0.423 CRELD1 1, mRNA. NM_001031717.1 3

Homo sapiens dehydrogenase/reductase (SDR family) member 3 (DHRS3), 0.424 DHRS3 mRNA. NM_004753.4 1

EVA1 Homo sapiens epithelial V-like antigen 1 (EVA1), transcript variant 2, mRNA. NM_144765.1 11 0.424

FZD2 Homo sapiens frizzled homolog 2 (Drosophila) (FZD2), mRNA. NM_001466.2 17 0.424

Homo sapiens ATPase, H+ transporting, lysosomal V0 subunit a isoform 1 0.426 ATP6V0A1 (ATP6V0A1), mRNA. NM_005177.2 17

SEC14L1 Homo sapiens SEC14-like 1 (S. cerevisiae) (SEC14L1), mRNA. NM_003003.1 17 0.43

STX16 Homo sapiens syntaxin 16 (STX16), transcript variant 2, mRNA. NM_003763.3 2 0.431

Homo sapiens solute carrier family 2 (facilitated glucose transporter), member 6 0.436 SLC2A6 (SLC2A6), mRNA. NM_017585.2 9

Homo sapiens ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,

3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 (ST6GALNAC4), 0.44

ST6GALNAC4 transcript variant 3, mRNA. NM_175040.1 9

PRSS16 Homo sapiens protease, serine, 16 (thymus) (PRSS16), mRNA. NM_005865.2 6 0.442

PDDC1 Homo sapiens Parkinson disease 7 domain containing 1 (PDDC1), mRNA. NM_182612.1 11 0.445

Homo sapiens phosphatidylinositol glycan, class N (PIGN), transcript variant 2, 0.447 PIGN mRNA. NM_012327.3 18

KIAA0746 Homo sapiens KIAA0746 protein (KIAA0746), mRNA. NM_015187.1 4 0.46 NAT6 Homo sapiens N-acetyltransferase 6 (NAT6), mRNA. NM_012191.2 3 0.462

PCGF2 Homo sapiens polycomb group ring finger 2 (PCGF2), mRNA. NM_007144.2 17 0.466

ACPL2 Homo sapiens acid phosphatase-like 2 (ACPL2), mRNA. NM_152282.2 3 0.467

Table S3. Differential pathway analysis (Time Point Zero Group): Compared to control, there are 1775 genes up-regulated and 1451 genes down-regulated. Data are presented as differential pathway analysis by gene expression.

Number Number Number Percent Percent MAPP Name Change Z Score PermuteP AdjustedP Measured On MAPP Changed Present d

helicase activity 34 122 131 27.86885 93.12977 3.564 0.001 0.234

negative regulation of cell 31 116 129 26.72414 89.92248 3.138 0.001 0.594 proliferation

DNA replication 34 123 146 27.64228 84.24657 3.51 0.002 0.247

cell cycle 39 156 173 25 90.17341 3.054 0.003 0.639

Hs_Cell_Cycle-G1_to_S_co 19 64 67 29.6875 95.52238 2.973 0.004 0.691 ntrol_Reactome

cysteine-type peptidase 27 102 125 26.47059 81.6 2.871 0.005 0.774 activity

Hs_Cell_cycle_KEGG 22 79 91 27.8481 86.81319 2.859 0.006 0.785

Hs_DNA_replication_React 13 39 42 33.33333 92.85714 2.94 0.007 0.725 ome

DNA repair 40 166 187 24.09639 88.77005 2.833 0.008 0.8

response to DNA damage 43 182 206 23.62637 88.34952 2.794 0.008 0.819 stimulus

apoptosis 52 236 255 22.0339 92.54902 2.516 0.013 0.971

Golgi apparatus 59 271 296 21.77122 91.55405 2.581 0.015 0.951

negative regulation of 35 149 160 23.48993 93.125 2.479 0.021 0.976 metabolism

mitochondrial inner 24 97 117 24.74227 82.90598 2.334 0.021 0.993 membrane

Hs_Fas_Pathway_and_Stre

ss_Induction_of_HSP_Regu 10 34 38 29.41176 89.47369 2.121 0.029 1

lation_Biocarta

Hs_Oxidative_Stress 8 24 28 33.33333 85.71429 2.305 0.032 0.998

regulation of cell cycle 38 172 187 22.09302 91.97861 2.164 0.032 1

Hs_Apoptosis 19 75 82 25.33333 91.46342 2.19 0.033 1

negative regulation of 32 141 148 22.69504 95.27027 2.153 0.036 1 cellular metabolism

cysteine-type 21 86 105 24.4186 81.90476 2.115 0.037 1 endopeptidase activity

mitochondrial membrane 29 127 152 22.83465 83.55264 2.085 0.045 1

Table S4. Differential pathway analysis (Time Point Two Group): Compared to control there are 1004 genes up-regulated and 1177 genes down-regulated. Data are presented as differential pathway analysis by gene expression.

Number Number Number Percent Percent MAPP Name Change Measure Z Score PermuteP AdjustedP On MAPP Changed Present d d

lysosome 21 93 103 22.58064 90.29126 3.716 0.001 0.241

negative regulation of cell 22 116 129 18.96552 89.92248 2.889 0.005 0.835 proliferation

helicase activity 23 122 131 18.85246 93.12977 2.923 0.006 0.823

apoptosis 39 236 255 16.52542 92.54902 2.917 0.007 0.825

mitochondrial inner 17 97 117 17.52577 82.90598 2.18 0.024 0.999 membrane

I-kappaB kinase 17 96 105 17.70833 91.42857 2.227 0.031 0.999 NF-kappaB cascade

structural constituent of 16 92 104 17.3913 88.46154 2.081 0.042 1 cytoskeleton

Table S5. Differential pathway analysis (Time Point Six Group): Compared to control there are 1129 genes up-regulated and 1207 genes down-regulated. Data are presented as differential pathway analysis by gene expression.

Number Number Number Percent Percent MAPP Name Change Measure Z Score PermuteP AdjustedP On MAPP Changed Present d d

helicase activity 29 122 131 23.77049 93.12977 4.365 0 0.034

negative regulation of cell 26 116 129 22.41379 89.92248 3.792 0 0.19 proliferation

apoptosis 43 236 255 18.22034 92.54902 3.381 0 0.459

lysosome 22 93 103 23.65591 90.29126 3.772 0.001 0.194

mitochondrial inner 19 97 117 19.58763 82.90598 2.583 0.011 0.974 membrane

regulation of cell 39 236 252 16.52542 93.65079 2.552 0.014 0.978 proliferation

Hs_HSP70_and_Apoptosis 5 15 18 33.33333 83.33334 2.695 0.022 0.943

Hs_Inflammatory_Response 7 28 33 25 84.84849 2.29 0.038 1 _Pathway

negative regulation of 17 93 102 18.27957 91.17647 2.129 0.04 1 apoptosis

cysteine-type 16 86 105 18.60465 81.90476 2.143 0.041 1 endopeptidase activity

negative regulation of 17 94 103 18.08511 91.26214 2.081 0.043 1 programmed cell death

Hs_Apoptosis 14 75 82 18.66667 91.46342 2.017 0.049 1

Table S6. Differential pathway analysis (Time Point 12 Group): Compared to control there are 1217 genes up-regulated and 1386 genes down-regulated. Data are presented as differential pathway analysis by gene expression

Number Number Number Percent Percent MAPP Name Change Measure Z Score PermuteP AdjustedP On MAPP Changed Present d d

helicase activity 41 122 131 33.60656 93.12977 6.834 0 0

I-kappaB kinase NF-kappaB 23 96 105 23.95833 91.42857 3.229 0.001 0.517 cascade

chromatin 30 145 170 20.68966 85.29412 2.797 0.008 0.857

structural constituent of 20 92 104 21.73913 88.46154 2.524 0.013 0.978 cytoskeleton

Hs_Biotin_metabolism 3 5 11 60 45.45454 3.136 0.017 0.62

lysosome 20 93 103 21.50538 90.29126 2.471 0.025 0.987

apoptosis 42 236 255 17.79661 92.54902 2.244 0.038 0.999

chromatin binding 11 47 51 23.40425 92.15686 2.142 0.049 1

Table S7. Differential pathway analysis (Time Point Twenty Group): Compared to control there are 1002 genes up-regulated and 1301 genes down-regulated. Data are presented as differential pathway analysis by gene expression.

Number Number Number Percent Percent MAPP Name Change Measure Z Score PermuteP AdjustedP On MAPP Changed Present d d

apoptosis 42 236 255 17.79661 92.54902 3.535 0.002 0.364

chromatin 30 145 170 20.68966 85.29412 3.893 0.001 0.158

chromatin assembly 19 103 115 18.4466 89.56522 2.537 0.013 0.974

chromatin assembly or 26 137 152 18.9781 90.13158 3.132 0.004 0.607 disassembly

cytoskeleton 35 232 263 15.08621 88.21293 2.159 0.028 1

establishment and or

maintenance of chromatin 35 210 232 16.66667 90.51724 2.798 0.006 0.901

architecture

GTPase activity 25 142 157 17.60563 90.44586 2.658 0.014 0.948

Hs_G13_Signaling_Pathway 8 36 37 22.22222 97.29729 2.229 0.031 1

negative regulation of 18 93 102 19.35484 91.17647 2.694 0.012 0.946 apoptosis

negative regulation of cell 25 116 129 21.55172 89.92248 3.779 0 0.201 proliferation

negative regulation of 18 94 103 19.14894 91.26214 2.644 0.013 0.951 programmed cell death

nucleosome assembly 19 94 106 20.21277 88.67924 2.978 0.002 0.764

small GTPase regulator 21 122 133 17.21312 91.72932 2.321 0.033 0.998 activity

structural constituent of 16 92 104 17.3913 88.46154 2.069 0.048 1 cytoskeleton

Figure S1

Fig. S1. The result of differentially expressed genes related to negative regulation of cell proliferation. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)

Figure S2

Fig. S2. Differentially expressed gens related to cell cycle pathway. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)

Figure S3

Fig. S3. Differentially expressed genes related to the mitochondrial oxidative stress pathway. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)

Figure S4

Fig. S4. Differentially expressed genes related to mitochondrial inner membrane. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)

Figure S5

Fig. S5. Differentially expressed genes related to cysteine-type endopeptidase activity. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)

Figure S6

Fig. S6. Differentially expressed genes related to FAS pathway and stress induction of HSP regulation. (red, up-regulation; green, down-regulation; grey, not criteria met; blank, not found.)