Ligand specificity Carbohydrate Binding Module 64A, GFP-CBM64 binds to crystalline forms of cellulose. See the (GFP-CBM64) reference below for more details on the biochemical properties of GFP-CBM64. Spirochaeta thermophila Assay conditions Catalogue number(s): CZ00921, 1 mg To recover maximal GFP-CBM64 activity, centrifuge a CZ00922, 3 × 1 mg required volume of the precipitated protein suspension (13000 xg for 5 min), remove the ammonium sulphate supernatant and ressuspend the resulting pellet in the same

volume of 20 mM Tris-HCl, pH 7.5, 20 mM NaCl, 5 mM CaCl2. Description Proceed with the assay as required. GFP-CBM64 is a Carbohydrate Binding Protein derived from Spirochaeta thermophila. Recombinant GFP-CBM64, purified from Escherichia coli, is a modular protein containing a family 64 Carbohydrate Binding Module (CBM64) and an N-terminal Green Fluorescent Protein (GFP) (www.cazy.org). This GFP protein derivative is particularly recommended for subcellular localization studies. The protein is provided in 3.2 M ammonium sulphate at a 1 mg/mL concentration. Bulk Quality control assay quantities of this product are available on request. Protein purity is >90% as judged by SDS-PAGE followed by BlueSafe staining (MB15201). Electrophoretic Purity GFP-CBM64 purity was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by BlueSafe staining (MB15201) (Figure 1). Certificate of Analysis

Test Result

Enzyme purity Pass

Approved by: José Prates Senior Manager, Quality Systems

Revised 04/15

NZYTech Ltd Estrada do Paço do Lumiar, 22 Campus do Lumiar, Edifício E, R/C 1649-038 Lisboa, Portugal Tel.:+351 213643514 Figure 1. SDS-PAGE analysis of GFP-CBM64 (Lane 1). Fax: +351 217151168 Electrophoresis was performed using a 14% polyacrylamide www.nzytech.com gel. The Mw of the protein is 39,1 kDa. Lane M contains NZYTech Low Molecular Weight (LMW) Protein Marker (MB082).

Storage temperature This protein is shipped at room temperature but should be stored at 4 °C.