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29733 MBNL2 Antibody Revision 1 C 0 2 - t MBNL2 Antibody a e r o t S Orders: 877-616-CELL (2355) [email protected] 3 Support: 877-678-TECH (8324) 3 7 Web: [email protected] 9 www.cellsignal.com 2 # 3 Trask Lane Danvers Massachusetts 01923 USA For Research Use Only. Not For Use In Diagnostic Procedures. Applications: Reactivity: Sensitivity: MW (kDa): Source: UniProt ID: Entrez-Gene Id: WB H Endogenous 41 Rabbit Q5VZF2 10150 ( ) g g p ( ) g Product Usage Information toxic RNA hairpins that sequester MBNLs in nuclear foci. This loss of available MBNLs causes an adult-to-fetal shift in alternative splicing patterns and ultimately results in Application Dilution respiratory and cardiac complications observed in DM patients (2,5). 1. Pascual, M. et al. (2006) Differentiation 74, 65-80. Western Blotting 1:1000 2. Konieczny, P. et al. (2014) Nucleic Acids Res 42, 10873-87. 3. Lee, K.Y. et al. (2013) EMBO Mol Med 5, 1887-900. Storage 4. Lee, K.S. et al. (2010) J Biol Chem 285, 33779-87. 5. Cho, D.H. and Tapscott, S.J. (2007) Biochim Biophys Acta 1772, 195-204. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Specificity / Sensitivity MBNL2 Antibody recognizes endogenous levels of total MBNL2 protein. This antibody does not cross-react with MBNL1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly119 of human MBNL2 protein. Antibodies are purified by peptide affinity chromatography. Background Alternative splicing is a crucial biological process that promotes protein diversity and provides cells with an additional mechanism to regulate the expression of tissue-specific protein isoforms. Muscleblind-like proteins (MBNLs) are one such protein family responsible for tissue-specific alternative splicing regulation. MBNLs bind pre-mRNA through an evolutionarily conserved zinc finger domain, and act as either activators or repressors of splicing on specific pre-mRNA targets by promoting the inclusion or exclusion of exons (1). MBNLs are functionally antagonistic to CUG-BP and ETR-3-like factors (CELF proteins) that also control pre-mRNA splicing. The interplay between MBNL and CELF activity plays a key role in development, where predominant MBNL activity promotes adult differentiation, and predominant CELF activity promotes embryonic splicing patterns (1). Three MBNL homologs are expressed in humans (MBNL1, MBNL2, and MBNL3) that share similar structure and function, yet differ in their tissue- and developmental stage-specific expression patterns (2). MBNL1 is the predominant isoform in the majority of tissue, including muscle, and is therefore the most well characterized. MBNL2 is the predominant homolog in brain, and exhibits increased expression upon functional loss of MBNL1 in other tissue types, suggesting a compensatory role (3). MBNL3 appears to play a more specialized role, where it inhibits muscle differentiation in muscle precursor cells (4). Functional loss of MBNLs is observed in myotonic dystrophy (DM), where pathological repeats in the 3’-UTR of the myotonic dystrophy protein kinase (DMPK) gene or intron 1 of the cellular nucleic acid binding protein (CNBP) gene result in Species reactivity is determined by testing in at least one approved application (e.g., IMPORTANT: For primary antibodies recommended for western blotting applications, western blot). we recommend incubating the membrane with diluted antibody at 4°C with gentle shaking overnight. Please refer to the western blot protocol found on the product web page for the antibody-specific diluent recommendation. APPLICATIONS KEY WB: Western Blot IP: Immunoprecipitation IHC: Immunohistochemistry ChIP: Chromatin Immunoprecipitation IF: Immunofluorescence F: Flow Cytometry E-P: ELISA-Peptide Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc. CROSS-REACTIVITY KEY H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse All: all species expected https://www.cellsignal.com/datasheet.jsp?productId=29733&images=0&protocol=0 1/2 Revision 1 #29733 MBNL2 Antibody Limited Uses Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products. CSTLT_86_20200512 Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: www.cellsignal.com https://www.cellsignal.com/datasheet.jsp?productId=29733&images=0&protocol=0 2/2.
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