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Produktinformation Produktinformation Diagnostik & molekulare Diagnostik Laborgeräte & Service Zellkultur & Verbrauchsmaterial Forschungsprodukte & Biochemikalien Weitere Information auf den folgenden Seiten! See the following pages for more information! Lieferung & Zahlungsart Lieferung: frei Haus Bestellung auf Rechnung SZABO-SCANDIC Lieferung: € 10,- HandelsgmbH & Co KG Erstbestellung Vorauskassa Quellenstraße 110, A-1100 Wien T. +43(0)1 489 3961-0 Zuschläge F. +43(0)1 489 3961-7 [email protected] • Mindermengenzuschlag www.szabo-scandic.com • Trockeneiszuschlag • Gefahrgutzuschlag linkedin.com/company/szaboscandic • Expressversand facebook.com/szaboscandic SAN TA C RUZ BI OTEC HNOL OG Y, INC . WDR78 (E-7): sc-390633 BACKGROUND APPLICATIONS WD-repeats are motifs that are found in a variety of proteins and are charac - WDR78 (E-7) is recommended for detection of WDR78 isoforms of mouse, terized by a conserved core of 40-60 amino acids that commonly form a terti - rat and human origin by Western Blotting (starting dilution 1:100, dilution ary propeller structure. While proteins that contain WD-repeats participate in range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total a wide range of cellular functions, they are generally involved in regulatory protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, mechanisms concerning chromatin assembly, cell cycle control, signal trans - dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, duction, RNA processing, apoptosis and vesicular trafficking. WDR78 (WD dilu tion range 1:30-1:3000). repeat domain 78) is an 848 amino acid protein that contains 6 WD repeats Suitable for use as control antibody for WDR78 siRNA (h): sc-78584, WDR78 and exists as 3 alternatively spliced isoforms. The gene encoding WDR78 siRNA (m): sc-155318, WDR78 shRNA Plasmid (h): sc-78584-SH, WDR78 maps to human chromosome 1, which spans 260 million base pairs, contains shRNA Plasmid (m): sc-155318-SH, WDR78 shRNA (h) Lentiviral Particles: over 3,000 genes and comprises nearly 8% of the human genome. sc-78584-V and WDR78 shRNA (m) Lentiviral Particles: sc-155318-V. REFERENCES Molecular Weight of WDR78 isoforms: 95/57/63 kDa. 1. van der Voorn, L. and Ploegh, H.L. 1992. The WD-40 repeat. FEBS Lett. Positive Controls: WDR78 (m2): 293T Lysate: sc-126238. 307: 131-134. 2. Neer, E.J., et al. 1994. The ancient regulatory-protein family of WD-repeat RECOMMENDED SUPPORT REAGENTS proteins. Nature 371: 297-300. To ensure optimal results, the following support reagents are recommended: 1) Western Blotting: use m-IgG BP-HRP: sc-516102 or m-IgG BP-HRP 3. Garcia-Higuera, I., et al. 1996. Folding of proteins with WD-repeats: com - κ κ (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ parison of six members of the WD-repeat superfamily to the G protein Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: subunit. Biochemistry 35: 13985-13994. β sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- 4. Smith, T.F., et al. 1999. The WD repeat: a common architecture for diverse itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). functions. Trends Biochem. Sci. 24: 181-185. 3) Immunofluorescence: use m-IgG κ BP-FITC: sc-516140 or m-IgG κ BP-PE: sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: 5. Li, D. and Roberts, R. 2001. WD-repeat proteins: structure characteristics, sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. biological function, and their involvement in human diseases. Cell. Mol. Life Sci. 58: 2085-2097. DATA 6. Koshizuka, Y., et al. 2001. Isolation, characterization, and mapping of the mouse and human WDR8 genes, members of a novel WD-repeat gene AB family. Genomics 72: 252-259. CHROMOSOMAL LOCATION 116 K – Genetic locus: WDR78 (human) mapping to 1p31.3; Wdr78 (mouse) mapping 81K– < WDR78 to 4 C6. SOURCE WDR78 (E-7): sc-390633. Western blot analysis of WDR78 (E-7) is a mouse monoclonal antibody specific for an epitope map ping WDR78 expression in non-transfected: sc-117752 (A) and mouse WDR78 transfected: sc-126238 (B) 293T between amino acids 459-482 within an internal region of WDR78 of human whole cell lysates. origin. RESEARCH USE PRODUCT For research use only, not for use in diagnostic procedures. Each vial contains 200 µg IgG 1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. PROTOCOLS Blocking peptide available for competition studies, sc-390633 P, (100 µg See our web site at www.scbt.com for detailed protocols and support peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer products. protein). STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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