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Epigenetics of Ancient DNA JULY-SEPTEMBER 2016 VOL. 8 № 3 (30) ISSN 2075-8251 Epigenetics of Ancient DNA ancient bone ancient DNA modern DNA STEM CELLS IN THE TREATMENT HYALURONIC ACID IN VASCULAR AND OF INSULIN-DEPENDENT IMMUNE HOMEOSTASIS DURING NORMAL DIABETES MELLITUS PREGNANCY AND PREECLAMPSIA Р. 31 Р. 59 Letter from the Editors ear readers of ActaNaturae, In the near future we intend to launch the We are delighted to bring you the 30th “electronic editorial board” system, which Dissue of the journal. will hopefully make communication between We continue to develop steadily. In 2015, authors and the editorial staff, peer review- our impact factor rose to 1.77, making Acta ers, and the Editorial Board more mobile, will Naturae third among Russian research jour- allow one to check manuscript status, to look nals in all disciplines. The credit goes to the through the comments of peer reviewers, and highly professional work of the editorial staff, to edit one’s personal data provided upon in- the Editorial Board, as well as to the increased itial registration. quality of the papers published. Gone are the We are looking forward to receiving new days when the Editorial Board had to put in a interesting manuscripts to publish with great lot of effort in attracting well-known authors pleasure! to submit articles. Today, with the portfolio See you in our next issue! full, we have an opportunity to select the sub- mitted papers more carefully and strictly. The Editorial Board VOL. 8 № 3 (30) 2016 | ACTA NATURAE | 1 INNOVATION RUSSIA Discussion club We create a dialogue between all socially active groups of people: students, scientists, lecturers, businessmen, managers, innovators, investors, designers, art critics, architects, photographs. Learn more at WWW.STRF.RU Everyone with something to say and ideas to share is welcome to visit our events Tel.: +7 (495) 930-87-07, 930-88-50 E-mail: [email protected] HIGHLIGHTS Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood E.V. Sotnezova, E.R. Andreeva, A.I. Grigoriev, L.B. Buravkova Suspended Transplantation of umbilical cord blood cells is widely used HSPCs in clinical practice. However, the limited number of hema- Adhered topoietic stem cells and prolonged time of recovery after the HSPCs transplantation are significant limitations for the use of the cord blood. The goal of the present review is to analyze the 50 µm HSPCs under MSCs recent methodological approaches to increase the effective- ness of using umbilical cord blood-derived hematopoietic Cultivation of cord blood mononuclear cells on mes- stem cells. enchymal stromal cells as a feeder layer. Collagen-coated (2D) culture vessel No gradients Apical-basal polarity of soluble induced by a stiff morphogenetic substrate Bioreactor-Based Tumor Tissue factors Blood vessel Continuous layer of matrix coating Tumor stem cells Engineering Unlimited potential for Increased stiffness Adhesions limited to a growth and migration (measured in GPa substrate plane only across a substrate plane range) Collagen gel (3D) A. E. Guller, P. N. Grebenyuk, A. B. Shekhter, A. V. Zvyagin, Presence of No certain cell gradients polarity of soluble morphogenet- Discrete S. M. Deyev ic factors matrix fibrils Tumor tissue engineering is a new method of three-dimensional (3D) simulation of malignant neoplasms whose main goal is to Fibroblast Spatially-hindered provide the maximally realistic reproduction and long-term growth and Low stiffness migration (measured in kPa range) maintenance of the simulated tumor properties in vitro to study Adhesive Tumor cells contacts are distributed over the entire cell cancer biology and to develop new methods for diagnosis and A B surface treatment of neoplasms. The review focuses on the problem of Structure of a malignant tumor and the changes bioreactor-based tissue-engineering simulation of malignant taking place during 2D and 3D cultivation of cells tumors. in vitro 100 ММ Cleavage of Human Embryos: Options and 80 60 2:2:2 2:2, 2:2:1 2:1:2 40 2:1, 2:1:1 1:2:2 Diversity 1:2, 1:2:1 20 1:1:2 1:1, 1:1:1 0 100 МЕ 80 Yu. K. Doronin, I. V. Senechkin, L. V. Hilkevich, M. A. Kurcer 60 The time-lapse imaging data of preimplantation human embryo development were 40 used to estimate the different forms of embryo cleavage relative to embryo progress 20 0 (blastocyst formation). These data on the early development of human embryos 100 ЕМ obtained using noninvasive methods complement and extend our understanding 80 of the embryogenesis of eutherian mammals and may be applied in the practice of 60 40 reproductive technologies. 20 0 4 5 6 7 8 9 10 11 12 13 14 15 Distribution of blasto- mere cleavage periods for 4-cell and 8-cell embryos. VOL. 8 № 3 (30) 2016 | ACTA NATURAE | 3 Indexed in Pubmed, Web of Science, Scopus, and RISC JULY-SEPTEMBER 2016 VOL. 8 № 3 (30) Founders Ministry of Education and Science of the Russian Federation, Lomonosov Moscow State University, CONTENTS Park Media Ltd Editorial Council Chairman: A.I. Grigoriev Editors-in-Chief: A.G. Gabibov, S.N. Kochetkov Letter from the Editors . 1 V.V. Vlassov, P.G. Georgiev, M.P. Kirpichnikov, A.A. Makarov, A.I. Miroshnikov, V.A. Tkachuk, M.V. Ugryumov REVIEWS Editorial Board Managing Editor: V.D. Knorre E.V. Sotnezova, E.R. Andreeva, A.I. Grigoriev, Publisher: K.V. Kiselev L.B. Buravkova K.V. Anokhin (Moscow, Russia) Ex Vivo Expansion of Hematopoietic Stem and I. Bezprozvanny (Dallas, Texas, USA) I.P. Bilenkina (Moscow, Russia) Progenitor Cells from Umbilical Cord Blood . .6 M. Blackburn (Sheffield, England) S.M. Deyev (Moscow, Russia) V.M. Govorun (Moscow, Russia) O. V. Markov, N. L. Mironova, V. V. Vlasov, O.A. Dontsova (Moscow, Russia) M. A. Zenkova K. Drauz (Hanau-Wolfgang, Germany) A. Friboulet (Paris, France) Molecular and Cellular Mechanisms of M. Issagouliants (Stockholm, Sweden) Antitumor Immune Response Activation by A.L. Konov (Moscow, Russia) M. Lukic (Abu Dhabi, United Arab Emirates) Dendritic Cells . 17 P. Masson (La Tronche, France) K. Nierhaus (Berlin, Germany) M. A. Borisov, O. S. Petrakova, I. G. Gvazava, V.O. Popov (Moscow, Russia) I.A. Tikhonovich (Moscow, Russia) E. N. Kalistratova, A. V. Vasiliev A. Tramontano (Davis, California, USA) Stem Cells in the Treatment of Insulin- V.K. Švedas (Moscow, Russia) J.-R. Wu (Shanghai, China) Dependent Diabetes Mellitus . 31 N.K. Yankovsky (Moscow, Russia) M. Zouali (Paris, France) A. E. Guller, P. N. Grebenyuk, A. B. Shekhter, Project Head: N.V. Soboleva A. V. Zvyagin, S. M. Deyev Editor: N.Yu. Deeva Designer: K.K. Oparin Bioreactor-Based Tumor Tissue Engineering .44 Art and Layout: K. Shnaider Copy Chief: Daniel M. Medjo M. M. Ziganshina, S. V. Pavlovich, N. V. Bovin, Address: 119234 Moscow, Russia, Leninskiye Gory, Nauchny G. T. Sukhikh Park MGU, vlad.1, stroeniye 75G. Phone/Fax: +7 (495) 727 38 60 Hyaluronic Acid in Vascular and Immune E-mail: [email protected], [email protected] Homeostasis during Normal Pregnancy and Reprinting is by permission only. Preeclampsia . 59 © ACTA NATURAE, 2016 Hoмep пoдпиcaн в пeчaть 26 мaя 2016 г. Tиpaж 200 экз. Цeнa cвoбoднaя. S. V. Zhenilo, A.S. Sokolov, E. B. Prokhortchouk Oтпeчaтaнo в типoгpaфии «MИГ ПPИHT» Epigenetics of Ancient DNA . 72 4 | ACTA NATURAE | VOL. 8 № 3 (30) 2016 CONTENTS D.K. Nilov, A.V. Kulikov, E.A. Prokhorova, RESEARCH ARTICLES V.K. Švedas Identification of New Structural Fragments A.P. Bonartsev, G. A. Bonartseva, V. L. Myshkina, V. V. Voinova, for the Design of Lactate Dehydrogenase A T. K. Mahina, I. I. Zharkova, S. G. Yakovlev, Inhibitors . 118 A. L. Zernov, E. V. Ivanova, E. A. Akoulina, E. S. Kuznetsova, V. A. Zhuikov, O. A. Smirnova, O. N. Ivanova, S. G. Alekseeva, V. V. Podgorskii, F. Sh. Mukhtarov, V. L. Tunitskaya, J. Jansons, I. V. Bessonov, M. N. Kopitsyna, M. G. Isaguliants, S. N. Kochetkov, A. V. Ivanov A. S. Morozov, E. Y. Milanovskiy, Z. N. Tyugay, Analysis of the Domains of Hepatitis C Virus G. S. Bykova, M. P. Kirpichnikov, K. V. Shaitan Core and NS5A Proteins that Activate the Biosynthesis of poly(3-hydroxybutyrate- Nrf2/ARE Cascade . 123 co-3-hydroxy-4-methylvalerate) by Strain Azotobacter chroococcum 7B . 77 A.P. Topolyan, M.A. Belyaeva, E.E. Bykov, P.V. Coodan, E.A. Rogozhin, D.A. Strizhevskaya, Yu. K. Doronin, I. V. Senechkin, L. V. Hilkevich, O.M. Ivanova, A.V. Ustinov, I.V. Mikhura, I.A. M. A. Kurcer Prokhorenko, V.A. Korshun, A.A. Formanovsky Cleavage of Human Embryos: Options and Derivatization of Aminoglycoside Antibiotics with Diversity . 88 Tris(2,6-dimethoxyphenyl)carbenium Ion . 128 N.A. Zolotarev, O.G. Maksimenko, O.V. Shamova, D.S. Orlov, M.S. Zharkova, P.G. Georgiev, A.N. Bonchuk S.V. Balandin, E.V. Yamschikova, D. Knappe, R. Hoffmann, V.N. Kokryakov, ZAD-Domain Is Essential for Nuclear T.V. Ovchinnikova Localization of Insulator Proteins in Drosophila Minibactenecins ChBac7 .Nα and ChBac7 .Nβ - melanogaster . 97 Antimicrobial Peptides from Leukocytes of the O.G. Kulakova, M.R. Kabilov, L.V. Danilova, Goat Capra hircus. 136 E.V. Popova, O.A. Baturina, E.Y. Tsareva, N.M. Baulina, I.S. Kiselev, A.N. Boyko, Guidelines for Authors . 147 A.V. Favorov, O.O. Favorova, V.V. Vlassov Whole-Genome DNA Methylation Analysis of Peripheral Blood Mononuclear Cells Epigenetics in Multiple Sclerosis Patients with Different of Ancient DNA Disease Courses . 103 Yu.O. Nikishina, A.Ya. Sapronova, M.V. Ugrumov ancient bone The Effect of Dopamine Secreted ancient DNA by the Brain into the Systemic Circulation modern DNA on Prolactin Synthesis by the Pituitary gland IMAGE ON THE COVER PAGE in Ontogenesis . 111 (see the article by Zhenilo et al.) VOL.
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