Spatiotemporal regulation of circular RNA expression during the developmentof skeletal muscle, subcutaneous fat, and liver in Ningxiang pigs Biao Li Hunan Agricultural University Yan Gong Hunan Agricultural University Yuebo Zhang Hunan Agricultural University Kyung Seok Kim Iowa State University Yu Xiao Hunan Agricultural University Qinghua Zeng Hunan Agricultural University Kang Xu Chinese Academy of Sciences Yehui Duan Chinese Academy of Sciences Jianhua He Hunan Agricultural University Haiming Ma (
[email protected] ) Hunan Agricultural University https://orcid.org/0000-0002-2702-2440 Jun He Hunan Agricultural University Research article Keywords: circRNAs, Ningxiang pig, proling, skeletal muscle, liver, subcutaneous fat Posted Date: December 11th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-124585/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/22 Abstract Background In recent years, thousands of different circular RNAs (circRNAs) have been identied through comparative analysis of different pig breeds. However, very few studies have investigated the spatiotemporal expression patterns of circRNA during organ development, which is crucial for functional and evolutionary analysis. Results In this study, we systematically analyzed circRNAs associated with fatty acid metabolism in the three main organs (muscle, fat and liver) a tfour growth time points (30 d, 90 d, 150 d and 210 d after birth) for Ningxiang pigs, awell-known native pig breed in China known for its excellent meat quality. We identied 61,683 circRNAs and analyzed their molecular characteristics, potential functions, and interactions with miRNAs. The circRNAs exhibited notable spatiotemporal specicity in the form of dynamic expression.