sign in sign up
<<
Home , Cyanopindolol, Dihydroalprenolol, MPPF, Rauwolscine, SUCNR1

e t a e h e c c Promis- p n g s n m e m e n S P o c G- cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for GPCRs 3 5-Hydroxytryptamine 5-HT 1A Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-310-A 8-Hydroxy-DPAT, [propyl-2,3-ring-1,2,3- H]- 5-HT 1A agonist NET929 3 AequoZen frozen ready to screen cells CHO-K1 X ES-310-AF MPPF, [methyl- H]- 5-HT 1A antagonist NET1109 ValiScreen transfected cell line CHO-K1 X X X ES-310-C Lysergic acid diethylamide, [N-methyl- 3H]- agonist NET638 cAMPZen frozen ready to screen cells CHO-K1 X ES-310-CF Lysergic acid diethylamide, 2-[ 125 I]iodo-(+)-, ([ 125 I]-LSD) agonist NEX199 Membrane preparation CHO-K1 X 6110501400UA Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 HEK293 EBNA X RBHS1AM400UA 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 WAY100635 [METHOXY- 3H] antagonist NET1164 3 5-HT 2A Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-313-A hydrochloride, [ethylene- H]-, (R41 468) 5-HT 2 antagonist NET791 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-313-AF DOI, [ I]-(±)- 5-HT 2 agonist NEX255 3 ValiScreen transfected cell line CHO-K1 X X ES-313-C , [benzene ring- H]-, (Spiroperidol) 5-HT 2 antagonist NET565 Membrane preparation CHO-K1 X ES-313-M400UA Lysergic acid diethylamide, [N-methyl- 3H]- agonist NET638 Lysergic acid diethylamide, 2-[ 125 I]iodo-(+)-, ([ 125 I]-LSD) agonist NEX199 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 3 5-HT 2B Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-314-A Ketanserin hydrochloride, [ethylene- H]-, (R41 468) 5-HT 2 antagonist NET791 125 ValiScreen transfected cell line CHO-K1 X X ES-314-C DOI, [ I]-(±)- 5-HT 2 agonist NEX255 3 Membrane preparation CHO-K1 X ES-314-M400UA Spiperone, [benzene ring- H]-, (Spiroperidol) 5-HT 2 antagonist NET565 Lysergic acid diethylamide, [N-methyl- 3H]- agonist NET638 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 Lysergic acid diethylamide, 2-[ 125 I]iodo-(+)-, ([ 125 I]-LSD) agonist NEX199 Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 5-Hydroxy , [ 3H] endogenous NET1167 3 5-HT non edited isoform Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-318-A Ketanserin hydrochloride, [ethylene- H]-, (R41 468) 5-HT 2 antagonist NET791 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-318-AF DOI, [ I]-(±)- 5-HT 2 agonist NEX255 3 ValiScreen transfected cell line 1321N1 X X ES-318-C Spiperone, [benzene ring- H]-, (Spiroperidol) 5-HT 2 antagonist NET565 Membrane preparation HEK293 X 6110548400UA Lysergic acid diethylamide, [N-methyl- 3H]- agonist NET638 1321N1 X ES-318-M400UA Lysergic acid diethylamide, 2-[ 125 I]iodo-(+)-, ([ 125 I]-LSD) agonist NEX199 3 5-HT 2C edited isoform Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-315-A Hydroxytryptamine creatinine sulfate, 5-[1,2- H(N)]- endogenous NET498 3 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-315-AF 5-Carboxamidotryptamine, [1,2- H]- agonist NET1071 ValiScreen transfected cell line CHO-K1 X X X ES-315-C Membrane preparation CHO-K1 X ES-315-M400UA

5-HT 3A (ligand-gated ion channel) see Ion Channels 3 5-HT 5A Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-401-A Lysergic acid diethylamide, [N-methyl- H]- agonist NET638 125 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-401-AF Lysergic acid diethylamide, 2-[ I]iodo-(+)-, ([ I]-LSD) agonist NEX199 ValiScreen transfected cell line CHO-K1 X ES-401-C Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 Membrane preparation CHO-K1 X RBHS5AM400UA 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 CHO-K1 X ES-401-M400UA 3 5-HT 6 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-316-A Lysergic acid diethylamide, [N-methyl- H]- agonist NET638 125 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-316-AF Lysergic acid diethylamide, 2-[ I]iodo-(+)-, ([ I]-LSD) agonist NEX199 ValiScreen transfected cell line CHO-K1/1321N1 X X ES-316-C Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 cAMPZen frozen ready to screen cells 1321N1 X ES-316-CF 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 Membrane preparation CHO-K1 X ES-316-M400UA SB258585, [ 125 I]- antagonist NEX424 HEK293 X RBHS6M400UA Rat Membrane preparation HEK293 X RBRS6M400UA 3 5-HT 7 Gs Human Membrane preparation CHO-K1 X 6110512400UA Lysergic acid diethylamide, [N-methyl- H]- agonist NET638 Rat Membrane preparation HEK293 X RBRS7M400UA Lysergic acid diethylamide, 2-[ 125 I]iodo-(+)-, ([ 125 I]-LSD) agonist NEX199 5-Carboxamidotryptamine, [1,2- 3H]- agonist NET1071 Hydroxytryptamine creatinine sulfate, 5-[1,2- 3H(N)]- endogenous NET498 3 (Muscarinic) M1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-210-A Scopolamine methyl chloride, [N-methyl- H]- M1-M 5 antagonist NET636 AequoZen frozen ready to screen cells CHO-K1 X ES-210-AF Acetylcholine iodide, [acetyl- 3H]- endogenous NET113 3 ValiScreen transfected cell line CHO-K1 X X ES-210-C Quinuclidinyl benzilate, L-[benzilic-4,4'- H]- M1-M 5 antagonist NET656 3 Membrane preparation CHO-K1 X RBHM1M400UA Oxotremorine-M acetate, [methyl- H]- M1-M 5 agonist NET671 3 Pirenzepine, [N-methyl- H]- M1 antagonist NET780 3 4-DAMP, [N-methyl- H]- M1, M 3 antagonist NET1040 3 M2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-211-A Scopolamine methyl chloride, [N-methyl- H]- M1-M 5 antagonist NET636 AequoZen frozen ready to screen cells CHO-K1 X ES-211-AF Acetylcholine iodide, [acetyl- 3H]- endogenous NET113 3 ValiScreen transfected cell line CHO-K1 X X ES-211-C Quinuclidinyl benzilate, L-[benzilic-4,4'- H]- M1-M 5 antagonist NET656

Membrane preparation CHO-K1 X RBHM2M400UA Oxotremorine-M acetate, [methyl-3H]- M1-M 5 agonist NET671 3 AF-DX 384, [2, 3-dipropylamino- H]- M2 antagonist NET1041 3 M3 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-212-A Scopolamine methyl chloride, [N-methyl- H]- M1-M 5 antagonist NET636 AequoZen frozen ready to screen cells CHO-K1 X ES-212-AF Acetylcholine iodide, [acetyl- 3H]- endogenous NET113 3 ValiScreen transfected cell line CHO-K1 X X ES-212-C Quinuclidinyl benzilate, L-[benzilic-4,4'- H]- M1-M 5 antagonist NET656 3 Membrane preparation CHO-K1 X RBHM3M400UA Oxotremorine-M acetate, [methyl- H]- M1-M 5 agonist NET671 3 4-DAMP, [N-methyl- H]- M1, M 3 antagonist NET1040 3 M4 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-213-A Scopolamine methyl chloride, [N-methyl- H]- M1-M 5 antagonist NET636 3 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-213-AF Acetylcholine iodide, [acetyl- H]- endogenous NET113 3 ValiScreen transfected cell line CHO-K1 X X ES-213-C Quinuclidinyl benzilate, L-[benzilic-4,4'- H]- M1-M 5 antagonist NET656 3 Membrane preparation CHO-K1 X RBHM4M400UA Oxotremorine-M acetate, [methyl- H]- M1-M 5 agonist NET671 3 M5 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-214-A Scopolamine methyl chloride, [N-methyl- H]- M1-M 5 antagonist NET636 AequoZen frozen ready to screen cells CHO-K1 X X ES-214-AF Acetylcholine iodide, [acetyl- 3H]- endogenous NET113 3 ValiScreen transfected cell line CHO-K1 X X ES-214-C Quinuclidinyl benzilate, L-[benzilic-4,4'- H]- M1-M 5 antagonist NET656 3 Membrane preparation CHO-K1 X RBHM5M400UA Oxotremorine-M acetate, [methyl- H]- M1-M 5 agonist NET671

November 2011 Page 1 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Adenosine A1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-010-A Cyclopentyl-1,3-dipropylxanthine, 8-[dipropyl-2,3- H(N)]- A1 antagonist NET974 3 AequoZen frozen ready to screen cells CHO=K1 X ES-010-AF Ethylcarboxamidoadenosine, 5'-N-[adenine-2,8- H]-, (NECA) A1, A 2 agonist NET811 ValiScreen transfected cell line CHO-K1 X X X ES-010-C cAMPZen frozen ready to screen cells CHO-K1 X ES-010-CF Membrane preparation CHO-K1 X X ES-010-M400UA Rat Membrane preparation CHO-K1 X 6110511400UA 3 A2A Gs Human AequoScreen aequorin double-transfected cell line HEK293 Gα16 X X X ES-011-A CGS 21680, [carboxyethyl- H(N)]- A2 agonist NET1021 3 AequoZen frozen ready to screen cells HEK293 Gα16 X ES-011-AF Ethylcarboxamidoadenosine, 5'-N-[adenine-2,8- H]-, (NECA) A1, A 2 agonist NET811 ValiScreen transfected cell line HEK293 X X ES-011-C cAMPZen frozen ready to screen cells HEK293 X ES-011-CF Membrane preparation HEK293 X RBHA2AM400UA Rat ValiScreen transfected cell line CHO-K1 X MCL-511 3 A2B Gs Human AequoScreen aequorin double-transfected cell line HEK293 Gα16 X ES-013-A CGS 21680, [carboxyethyl- H(N)]- A2 agonist NET1021 3 ValiScreen transfected cell line HEK293 X X ES-013-C Ethylcarboxamidoadenosine, 5'-N-[adenine-2,8- H]-, (NECA) A1, A 2 agonist NET811 cAMPZen frozen ready to screen cells HEK293 X ES-013-CF Membrane preparation HEK293 X ES-013-M400UA 125 A3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-012-A AB-MECA, (4-amino-3-[ I]iodobenzyl-5'-N-)-methylcarboxamideoadenosine A3 agonist NEX312

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-012-AF ValiScreen transfected cell line CHO-K1 X X X ES-012-C cAMPZen frozen ready to screen cells CHO-K1 X ES-012-CF

PhotoScreen Photina double-transfected cell line CHO-K1 Gα16 X AX-001-PCF Membrane preparation CHO-K1 X X ES-012-M400UA Rat ValiScreen transfected cell line CHO-K1 X MCL-512 Membrane preparation HEK293 EBNA X RBRA3M400UA 3 Adrenoceptors α1A Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-036-A , [7-methoxy- H]- α1 antagonist NET823 125 AequoZen frozen ready to screen cells CHO-K1 X ES-036-AF HEAT, [ I]- α1 antagonist NEX182 125 ValiScreen transfected cell line CHO-K1 X X ES-036-C Prazosin analog [ I]-iodoazido-, (iodoarylazidoprazosin) α1 antagonist NEX219 Membrane preparation CHO-K1 X ES-036-M400UA

α1B Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-037-A

α1D Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-038-A 3 α2A Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-030-A MK-912, [methyl- H]- α2 antagonist NET1059 125 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-030-AF Iodoclonidine, p-[ I]-(2-[(2,5-dichloro-4-[ I] phenyl) imino] imidazolidine) α2 agonist NEX253 3 ValiScreen transfected cell line CHO-K1 X X X ES-030-C hydrochloride, [benzene ring- H]- α2 agonist NET613 cAMPZen frozen ready to screen cells CHO-K1 X ES-030-CF Epinephrine, levo-[N-methyl- 3H]- endogenous NET623 3 Membrane preparation CHO-K1 X X ES-030-M400UA , [methyl- H]- α2 antagonist NET659 Sf9 X 6110113400UA , levo-[ring-2,5,6- 3H]- endogenous NET678 3 , [methyl- H]- α2 antagonist NET722 3 UK-14,304, [imidazolyl-4,5- H]- α2 agonist NET853 3 RX821002, [ H] α2 antagonist NET1153 3 α2B Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-031-A MK-912, [methyl- H]- α2 antagonist NET1059 125 125 AequoZen frozen ready to screen cells CHO-K1 X ES-031-AF Iodoclonidine, p-[ I]-(2-[(2,5-dichloro-4-[ I] phenyl) imino] imidazolidine) α2 agonist NEX253 3 ValiScreen transfected cell line CHO-K1 X X ES-031-C Clonidine hydrochloride, [benzene ring- H]- α2 agonist NET613 Membrane preparation CHO-K1 X X ES-031-M400UA Epinephrine, levo-[N-methyl- 3H]- endogenous NET623 3 Yohimbine, [methyl- H]- α2 antagonist NET659 Norepinephrine, levo-[ring-2,5,6- 3H]- endogenous NET678 3 Rauwolscine, [methyl- H]- α2 antagonist NET722 3 UK-14,304, [imidazolyl-4,5- H]- α2 agonist NET853 3 RX821002, [ H] α2 antagonist NET1153 3 α2C Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-032-A MK-912, [methyl- H]- α2 antagonist NET1059 125 125 ValiScreen transfected cell line CHO-K1 X X ES-032-C Iodoclonidine, p-[ I]-(2-[(2,6-dichloro-4-[ I]iodophenyl)imino]imidazolidine) α2 agonist NEX253 3 cAMPZen frozen ready to screen cells CHO-K1 X ES-032-CF Clonidine hydrochloride, [benzene ring- H]- α2 agonist NET613 Membrane preparation Sf9 X 6110114400UA Epinephrine, levo-[N-methyl- 3H]- endogenous NET623 3 CHO-K1 X ES-032-M400UA Yohimbine, [methyl- H]- α2 antagonist NET659 Norepinephrine, levo-[ring-2,5,6- 3H]- endogenous NET678 3 Rauwolscine, [methyl- H]- α2 antagonist NET722 3 UK-14,304, [imidazolyl-4,5- H]- α2 agonist NET853 3 RX821002, [ H] α2 antagonist NET1153 3 β1 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-033-A (-)-CGP 12177, [5,7- H]- antagonist NET1061 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-033-AF (-)-, [ I]- antagonist NEX189 ValiScreen transfected cell line CHO-K1 X X ES-033-C (-)-, [ 125 I]- antagonist NEX211 cAMPZen frozen ready to screen cells CHO-K1 X ES-033-CF , L-[4- 3H]- antagonist NET515 Membrane preparation CHO-K1 X ES-033-M400UA hydrochloride, levo [ring, propyl- 3H]- antagonist NET720 Sf9 X 6110110400UA (+/-)-Cyanopindolol, [ 125 I]- antagonist NEX174

November 2011 Page 2 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Adrenoceptors (continued) β2 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-034-C (-)-CGP 12177, [5,7- H]- antagonist NET1061 cAMPZen frozen ready to screen cells CHO-K1 X ES-034-CF (-)-Cyanopindolol, [ 125 I]- antagonist NEX189 Membrane preparation CHO-K1 X ES-034-M400UA (-)-Pindolol, [ 125 I]- antagonist NEX211 Sf9 X 6110106400UA Propranolol, L-[4- 3H]- antagonist NET515 HEK293 EBNA X RBHBE2M400UA Dihydroalprenolol hydrochloride, levo [ring, propyl- 3H]- antagonist NET720 (+/-)-Cyanopindolol, [ 125 I]- antagonist NEX174 3 β3 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-035-C (-)-CGP 12177, [5,7- H]- antagonist NET1061 cAMPZen frozen ready to screen cells CHO-K1 X ES-035-CF (-)-Cyanopindolol, [ 125 I]- antagonist NEX189 Membrane preparation CHO-K1 X ES-035-M400UA (-)-Pindolol, [ 125 I]- antagonist NEX211 Non-recombinant, membrane preparation SK-N-MC X RBHBE3M400UA Propranolol, L-[4- 3H]- antagonist NET515 Dihydroalprenolol hydrochloride, levo [ring, propyl-3H]- antagonist NET720 (+/-)-Cyanopindolol, [ 125 I]- antagonist NEX174 125 Anaphylatoxin C3a Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-730-A Complement C3a , [ I]-, Bolton-Hunter labeled C3a endogenous NEX356 ValiScreen transfected cell line CHO-K1 X X X ES-730-C cAMPZen frozen ready to screen cells CHO-K1 X ES-730-CF Membrane preparation CHO-K1 X X ES-730-M400UA 125 C5a Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-731-A Complement C5a , [ I]-, Bolton-Hunter labeled C5a endogenous NEX250 AequoZen frozen ready to screen cells CHO-K1 X ES-731-AF ValiScreen transfected cell line CHO-K1 X X X ES-731-C cAMPZen frozen ready to screen cells CHO-K1 X ES-731-CF Membrane preparation CHO-K1 X X ES-731-M400UA CHO-K1 X 6110526400UA 1 4 8 125 Angiotensin AT 1 Gq/11 , G i/o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-072-A Angiotensin II (Sar , Tyr , Ile ), [ I]- AT antagonist NEX248 4 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-072-AF Angiotensin I (Tyr ), [ I]- endogenous NEX101 2 125 ValiScreen transfected cell line CHO-K1 X X ES-072-C Angiotensin IV (Tyr ), [ I]- Non AT 1, non AT 2 agonist NEX295 Membrane preparation Sf9 X 6110121400UA Angiotensin II (Tyr 4), [ 125 I]- endogenous NEX105 CHO-K1 X ES-072-M400UA 1 4 8 125 AT 2 Gi/G o Human ValiScreen transfected cell line CHO-K1 X ES-070-C Angiotensin II (Sar , Tyr , Ile ), [ I]- AT antagonist NEX248 2 125 Membrane preparation CHO-K1 X ES-070-M400UA Angiotensin IV (Tyr ), [ I]- Non AT 1, non AT 2 agonist NEX295 Mouse ValiScreen transfected cell line CHO-K1 X ES-071-C Angiotensin II (Tyr 4), [ 125 I]- endogenous NEX105 125 Membrane preparation CHO-K1 X ES-071-M400UA CGP42112A, [ I]- AT 2 antagonist NEX324 65 75 77 125 Apelin APJ Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-460-A Apelin 13 (Glp , Nle , Tyr ), [ I]- APJ agonist NEX393 ValiScreen transfected cell line CHO-K1 X X X ES-460-C cAMPZen frozen ready to screen cells CHO-K1 X ES-460-CF Membrane preparation CHO-K1 X X ES-460-M400UA

Beta Alanine MRGPRD, TGR7 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-741-A 4 125 Bombesin BB 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-581-A Bombesin (Tyr ), [ I]- agonist NEX258

Membrane preparation CHO-K1 Gα16 X RBHBS1M400UA 4 125 BB 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-582-A Bombesin (Tyr ), [ I]- agonist NEX258

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-582-AF ValiScreen transfected cell line CHO-K1 X X ES-582-C Gastrin Releasing Peptide (Tyr 15 ) (Porcine), [125I]- agonist NEX421 Membrane preparation HEK293 X RBHBS2M400UA CHO-K1 X ES-582-M400UA 6 11 13 14 125 BB 3 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-580-A Bombesin (6-14) (D-Tyr - βAla , Phe , Nle ), [ I]- BB 3 agonist NEX377 Membrane preparation Balb 3T3 X RBHBS3M400UA Bombesin (Tyr 4), [ 125 I]- agonist NEX258 10 3 Bradykinin B1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-091-A Kallidin (Des-Arg ), [3,4-prolyl-3,4- H(N)]- B1 agonist NET1064 10 9 3 ValiScreen transfected cell line CHO-K1 X X ES-091-C Kallidin (Des-Arg , Leu ), [3,4-prolyl-3,4- H(N)]- B1 antagonist NET1096 Membrane preparation CHO-K1 X ES-091-M400UA

B1 Gq/G 11 Rat AequoScreen aequorin double-transfected cell line CHO-K1 X ES-092-A 3 B2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-090-A Bradykinin, [2,3-prolyl-3,4- H]- endogenous NET706 ValiScreen transfected cell line CHO-K1 X X ES-090-C Membrane preparation CHO-K1 X ES-090-M400UA 125 125 Calcitonin CGRP 1 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαq/s X X X ES-420-A Calcitonin Related Peptide , [ I]-, ([ I]-hCGRP) CGPR 1, endogenous NEX354 125 125 AequoZen frozen ready to screen cells CHO-K1 Gαq/s X ES-420-AF Calcitonin Gene Related Peptide (8-37), [ I]-, ([ I]-hCGRP) CGPR 1 antagonist NEX319

ValiScreen transfected cell line CHO-K1 X X ES-420-C Amylin (r) [125I]- CGPR 1 antagonist NEX448 cAMPZen frozen ready to screen cells CHO-K1 X ES-420-CF Membrane preparation CHO-K1 X ES-420-M400UA

Sf9 Gαsβ1γ2 X 6110135400UA Non-recombinant, membrane preparation SK-N-MC X RBHGRPM400UA CT Calcitonin (Tyr 12 ) (human), [ 125 I]- endogenous NEX422 Calcitonin (salmon), [ 125 I]- NEX423 125 AM 1 Gs Human Membrane preparation Sf9 Gαsβ1γ2 X 6110136400UA Adrenomedullin (13-52), (Tyr), [ I]- AM 1, AM 2, endogenous NEX385 125 Adrenomedullin (rat), [ I]- AM 1, AM 2, endogenous NEX427 125 AM 2 Gs, G i/o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-430-A Adrenomedullin (13-52), (Tyr), [ I]- AM 1, AM 2, endogenous NEX385 125 ValiScreen transfected cell line CHO-K1 X X ES-430-C Adrenomedullin (rat), [ I]- AM 1, AM 2, endogenous NEX427 cAMPZen frozen ready to screen cells CHO-K1 X ES-430-CF Membrane preparation CHO-K1 X ES-430-M400UA

November 2011 Page 3 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Cannabinoid CB 1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-110-A CP 55940, [side chain-2,3,4- H(N)]- agonist NET1051 3 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-110-AF WIN 55,212-2, [5,7-naphtyl- H]- agonist NET1058 ValiScreen transfected cell line CHO-K1 X X X ES-110-C Anadamide, [arachidonyl-5,6,8,9,11,12,14,15- 3H]- CB, endogenous NET1073 3 cAMPZen frozen ready to screen cells CHO-K1 X ES-110-CF SR141716A, [ H] CB 1 antagonist NET1158 Membrane preparation CHO-K1 X X ES-110-M400UA

Sf9 Gαi3 β1γ2 X 6110129400UA HEK293 EBNA X RBHCB1M400UA 3 CB 2 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X X ES-111-C CP 55940, [side chain-2,3,4- H(N)]- agonist NET1051 cAMPZen frozen ready to screen cells CHO-K1 X ES-111-CF WIN 55,212-2, [5,7-naphtyl- 3H]- agonist NET1058 3 Membrane preparation Sf9 Gαi3 β1γ2 X 6110130400UA Anadamide, [arachidonyl-5,6,8,9,11,12,14,15- H]- CB, endogenous NET1073 HEK293 EBNA X RBXCB2M400UA CHO-K1 X X ES-111-M400UA

Chemerin GPR1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-661-A

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-661-AF 125 Chemokine CCR1 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X ES-132-C MIP-1 α, [ I]- CCR1, CCR4, CCR5, endogenous NEX298 cAMPZen frozen ready to screen cells CHO-K1 X ES-132-CF RANTES, [ 125 I]- CCR1, CCR3, CCR4, CCR5, endogenous NEX292 Membrane preparation HEK293 X RBHCC1M400UA 125 CCR2b Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-133-A MCP-1, [ I]-, Bolton-Hunter labeled CCR2, endogenous NEX332 AequoZen frozen ready to screen cells CHO-K1 X ES-133-AF JE, [ 125 I]-, Bolton-Hunter labeled (murine, recombinant MCP-1) CCR2 agonist NEX313 ValiScreen transfected cell line CHO-K1 X X X ES-133-C Membrane preparation CHO-K1 X X ES-133-M400UA HEK293 X 6110550400UA 125 CCR3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-138-A Eotaxin, [ I]- CCR3, endogenous NEX314

AequoZen frozen ready to screen cells CHO-K1 Gα16 X X ES-138-AF ValiScreen transfected cell line K562 X X ES-138-C Membrane preparation CHO-K1 X X ES-138-M400UA CCR4 TARC, [ 125 I]- endogenous NEX369 3 47 125 CCR5 Gi/G o MIP-1 β (Leu ,Gly ), [ I]- endogenous NEX299 125 CCR6 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-139-A MIP-3 α, [ I]- CCR6, endogenous NEX371 AequoZen frozen ready to screen cells CHO-K1 X ES-139-AF ValiScreen transfected cell line CHO-K1 X X X ES-139-C cAMPZen frozen ready to screen cells CHO-K1 X ES-139-CF Membrane preparation CHO-K1 X X ES-139-M400UA 125 CCR7 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-140-A MIP-3 β, [ I]- CCR7, endogenous NEX370 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-140-AF 6-Ckine, [ I]-, Bolton-Hunter labeled CCR7, endogenous NEX387 ValiScreen transfected cell line CHO-K1 X X X ES-140-C cAMPZen frozen ready to screen cells CHO-K1 X ES-140-CF Membrane preparation CHO-K1 X X ES-140-M400UA 125 CCR8 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-136-A I309, [ I]-, Bolton-Hunter labeled CCR8, endogenous NEX364 AequoZen frozen ready to screen cells CHO-K1 X ES-136-AF ValiScreen transfected cell line CHO-K1 X X X ES-136-C cAMPZen frozen ready to screen cells CHO-K1 X ES-136-CF Membrane preparation CHO-K1 X X ES-136-M400UA

CCR9a Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-146-A AequoZen frozen ready to screen cells CHO-K1 X ES-146-AF 125 CCR10 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-143-A CTACK, [ I]- CCR10, endogenous NEX391

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-143-AF 125 CX 3CR1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-137-A Fractalkine, [ I]- CX 3CR1, endogenous NEX368

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-137-AF ValiScreen transfected cell line CHO-K1 X X X ES-137-C cAMPZen frozen ready to screen cells CHO-K1 X ES-137-CF Membrane preparation CHO-K1 X X ES-137-M400UA 125 125 CXCR2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-145-A Interleukin-8, [ I]-, ([ I]-IL-8) CXCR1, CXCR2, endogenous NEX277 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-145-AF GRO Alpha/MGSA, [ I]- CXCR2, endogenous NEX321 ValiScreen transfected cell line CHO-K1 X X X ES-145-C cAMPZen frozen ready to screen cells CHO-K1 X ES-145-CF Membrane preparation CHO-K1 X X ES-145-M400UA 125 CXCR3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-142-A I-TAC, [ I]- CXCR3, endogenous NEX376 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-142-AF IP-10, [ I]- CXCR3, endogenous NEX348 ValiScreen transfected cell line CHO-K1 X X X ES-142-C Membrane preparation CHO-K1 X X ES-142-M400UA CXCR4 SDF1 α, [ 125 I]- CXCR4, endogenous NEX346 125 CXCR6 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-720-A CXCL16, [ I]- CXCR6, endogenous NEX398 AequoZen frozen ready to screen cells CHO-K1 X ES-720-AF ValiScreen transfected cell line CHO-K1 X X ES-720-C cAMPZen frozen ready to screen cells CHO-K1 X ES-720-CF Membrane preparation CHO-K1 X ES-720-M400UA HEK293 X RBHCX6M400UA

November 2011 Page 4 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for

Chemokine (continued) XCR1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-148-A

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-148-AF ValiScreen transfected cell line CHO-K1 X X ES-148-C cAMPZen frozen ready to screen cells CHO-K1 X ES-148-CF Membrane preparation CHO-K1 X ES-148-M400UA 125 125 Cholecystokinin CCK 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line 1321N1 X X X ES-530-A Cholecystokinin octapeptide, [ I]-, Bolton-Hunter labeled, ([ I]]-CCK-8) endogenous NEX203 AequoZen frozen ready to screen cells 1321N1 X ES-530-AF Gastrin I (Tyr 12 ), [ 125 I]- endogenous NEX176 ValiScreen transfected cell line 1321N1 X X ES-530-C Interleukin-8 (IL-8) (h,r) Receptor Grade, [ 125 I] agonist NEX443 Membrane preparation 1321N1 X ES-530-M400UA CCK-8, [ 3H] agonist NET1162 125 125 CCK 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line 1321N1 X X X ES-531-A Cholecystokinin octapeptide, [ I]-, Bolton-Hunter labeled, ([ I]-CCK-8) endogenous NEX203 AequoZen frozen ready to screen cells 1321N1 X ES-531-AF Gastrin I (Tyr 12 ), [ 125 I]- endogenous NEX176 ValiScreen transfected cell line 1321N1 X X ES-531-C Interleukin-8 (IL-8) (h,r) Receptor Grade, [ 125 I] agonist NEX443 Membrane preparation 1321N1 X ES-531-M400UA CCK-8, [ 3H] agonist NET1162 HEK293 X RBHCKBM400UA 0 125 Corticotropin- CRF 1 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-152-A Sauvagine (Tyr ), [ I]- endogenous NEX306 Releasing Factor ValiScreen transfected cell line CHO-K1 X X ES-152-C CRF (Tyr 0) (ovine), [ 125 I]- CRF agonist NEX217 Membrane preparation HEK293 X RBHCRF1M400UA CRF (Tyr 0) (human,rat), [ 125 I]- endogenous NEX216 CHO-K1 X ES-152-M400UA 3 D1 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-172-C SCH 23390, [N-methyl- H]- D1, D 5 antagonist NET930 cAMPZen frozen ready to screen cells CHO-K1 X ES-172-CF Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- 3H]-, (Dopamine) endogenous NET673 Membrane preparation L Cells X 6110513400UA Dihydroxyphenylethylamine, 3,4-[7- 3H]- endogenous NET131 CHO-K1 X ES-172-M400UA Dihydroxyphenylethylamine [2,5,6,7,8- 3H] (Dopamine) endogenous NET1160 125 D2L Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαqi/5 X ES-171-A Iodospiperone, 2'-[ I]- D2 antagonist NEX284 125 AequoZen frozen ready to screen cells CHO-K1 Gαqi/5 X ES-171-AF R (+) trans-7-hydroxy-PIPAT, [ I]- D2, D 3 agonist NEX307 3 Membrane preparation Sf9 X 6110137400UA Spiperone, [benzene ring- H]-, (Spiroperidol) D2 antagonist NET565 Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- 3H]-, (Dopamine) endogenous NET673 125 Iodosulpride, [ I]- D2, D 3 antagonist NEX441 3 (-)Sulpiride, [methoxy- H]- D2 antagonist NET775 3 Methylspiperone, [N-methyl- H]- D2, D 3 antagonist NET856 3 Raclopride, [methoxy- H]- D2 antagonist NET975 3 YM-09151-2, [N-methyl- H]- D2 antagonist NET1004 Dihydroxyphenylethylamine, 3,4-[7- 3H]- endogenous NET131 125 (DAN-2163), [ I]- D2, D 3 antagonist NEX432 3 Domperidone, [benzene ring- H]- D2, D 3 antagonist NET662 Quinpirole, [N-propyl- 3H]- 125 D2S Gi/G o Human Membrane preparation CHO-K1 X RBHD2CM400UA Iodospiperone, 2'-[ I]- D2 antagonist NEX284 125 R (+) trans-7-hydroxy-PIPAT, [ I]- D2, D 3 agonist NEX307 3 Spiperone, [benzene ring- H]-, (Spiroperidol) D2 antagonist NET565 Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- 3H]-, (Dopamine) endogenous NET673 125 Iodosulpride, [ I]- D2, D 3 antagonist NEX441 3 (-)Sulpiride, [methoxy- H]- D2 antagonist NET775 3 Methylspiperone, [N-methyl- H]- D2, D 3 antagonist NET856 3 Raclopride, [methoxy- H]- D2 antagonist NET975 3 YM-09151-2, [N-methyl- H]- D2 antagonist NET1004 Dihydroxyphenylethylamine, 3,4-[7- 3H]- endogenous NET131 125 Amisulpride (DAN-2163), [ I]- D2, D 3 antagonist NEX432 3 Domperidone, [benzene ring- H]- D2, D 3 antagonist NET662 3 Quinpirole, [N-propyl- H]- D2, D 3 agonist NET1085 3 D3 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X X ES-173-C Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- H]-, (Dopamine) endogenous NET673 Membrane preparation CHO-K1 X X ES-173-M400UA Dihydroxyphenylethylamine, 3,4-[7- 3H]- endogenous NET131 125 Rat Membrane preparation Sf9 X 6110139400UA R (+) trans-7-hydroxy-PIPAT, [ I]- D2, D 3 agonist NEX307 125 Iodosulpride, [ I]- D2, D 3 antagonist NEX441 3 Methylspiperone, [N-methyl- H]- D2, D 3 antagonist NET856 125 Amisulpride (DAN-2163), [ I]- D2, D 3 antagonist NEX432 3 Domperidone, [benzene ring- H]- D2, D 3 antagonist NET662 3 Quinpirole, [N-propyl- H]- D2, D 3 agonist NET1085 R(+)7-Hydroxy DPAT, [ 3H] agonist NET1169 3 D4 Gi/G o Rat ValiScreen transfected cell line CHO-K1 X X ES-170-C Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- H]-, (Dopamine) endogenous NET673 3 D4.2 Gi/G o Human Membrane preparation Sf9 X 6110112400UA Dihydroxyphenylethylamine, 3,4-[7- H]- endogenous NET131 CHO-K1 X RBHD42M400UA WAY100635 [METHOXY-3H]

D4.4 Gi/G o Human ValiScreen transfected cell line CHO-K1 X MCL-507 Membrane preparation Sf9 X 6110122400UA CHO-K1 X RBHD44M400UA

D4.7 Gi/G o Human Membrane preparation CHO-K1 X RBHD47M400UA 3 D5 Dihydroxyphenylethylamine, 3,4-[ring-2,5,6- H]-, (Dopamine) endogenous NET673 3 SCH 23390, [N-methyl- H]- D1, D 5 antagonist NET930 Dihydroxyphenylethylamine, 3,4-[7- 3H]- endogenous NET131

November 2011 Page 5 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 13 125 Endothelin ET A Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-320-A Endothelin-1 (Tyr ), [ I]- endogenous NEX259 AequoZen frozen ready to screen cells CHO-K1 X ES-320-AF ValiScreen transfected cell line CHO-K1 X ES-320-C Membrane preparation CHO-K1 X ES-320-M400UA 13 125 ET B Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-321-A Endothelin-1 (Tyr ), [ I]- endogenous NEX259 AequoZen frozen ready to screen cells CHO-K1 X ES-321-AF ValiScreen transfected cell line CHO-K1 X X ES-321-C Membrane preparation CHO-K1 X ES-321-M400UA 125 Formyl Peptide FPR2 (FPRL1) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-610-A WKYMVm, [ I]-, Bolton-Hunter labeled FPRL1 agonist NEX386

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-610-AF ValiScreen transfected cell line CHO-K1 X X X X ES-610-C cAMPZen frozen ready to screen cells CHO-K1 X ES-610-CF Membrane preparation CHO-K1 X X ES-610-M400UA

Free Fatty Acid FFA1 (GPR40) Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-652-A AequoZen frozen ready to screen cells CHO-K1 X ES-652-AF ValiScreen transfected cell line 1321N1 X ES-652-C FFA (GPR120) Gi/Go Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-800-A AequoZen frozen ready to screen cells CHO-K1 X ES-800-AF 3 GABA B GABA B1a Human Membrane preparation CHO-K1 X 6110545400UA Baclofen, (-)-[butyl-4- H(N)]- GABA B agonist NET867 HEK293 X 6110560400UA aminobutyric acid, γ -[2,3- 3H(N)]- endogenous NET191 3 GABA B1b Human Membrane preparation CHO-K1 X 6110546400UA aminobutyric acid, γ -[2,3- H(N)]- endogenous NET191X HEK293 X 6110557400UA Butyl bicyclophosphorothionate, tertiary-[ 35 S]- Cl-channel non competitive antagonist NEG049

GABA B1a/R2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-500-A ValiScreen transfected cell line CHO-K1 X X X ES-500-C cAMPZen frozen ready to screen cells CHO-K1 X ES-500-CF Membrane preparation HEK293 X 6110559400UA CHO-K1 X X ES-500-M400UA

GABA B1b/R2 Gi/G o Human Membrane preparation HEK293 X 6110556400UA 125 Galanin GAL 1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-510-A Galanin (porcine), [ I]- agonist NEX243

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-510-AF ValiScreen transfected cell line CHO-K1 X X X ES-510-C Galanin (human), [ 125 I]- endogenous NEX333 cAMPZen frozen ready to screen cells CHO-K1 X ES-510-CF Membrane preparation HEK293 EBNA X 6110537400UA CHO-K1 X X ES-510-M400UA 125 GAL 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-511-A Galanin (porcine), [ I]- agonist NEX243 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-511-AF Galanin (human), [ I]- endogenous NEX333 125 ValiScreen transfected cell line CHO-K1 X X X ES-511-C Galanin (2-11), [ I]- GAL 2, GAL 3 agonist NEX416 PhotoScreen Photina double-transfected cell line CHO-K1 X AX-008-PCF Membrane preparation CHO-K1 X ES-511-M400UA 125 Ghrelin ghrelin Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-410-A Ghrelin (His), [ I]- endogenous NEX388 AequoZen frozen ready to screen cells CHO-K1 X ES-410-AF Ghrelin (Tyr 4), [ 125 I]- agonist NEX417 ValiScreen transfected cell line CHO-K1 X X X ES-410-C Membrane preparation HEK293 X RBHGHSM400UA CHO-K1 X ES-410-M400UA 125 Glucagon glucagon Gs Human ValiScreen transfected cell line 1321N1 X X ES-710-C Glucagon, [ I]- endogenous NEX207 Membrane preparation 1321N1 X ES-710-M400UA AequoScreen aequorin double-transfected cell line CHO-K1 X ES-710-A 125 GIP Gs Human Membrane preparation HEK293 X RBHGIPM400UA Gastric Inhibitory Polypeptide, [ I]- endogenous NEX402 125 GLP-2 Gs Human ValiScreen transfected cell line 1321N1 X ES-711-C Exendin (9-39), [ I]- GLP-1 antagonist NEX335 cAMPZen frozen ready to screen cells 1321N1 X ES-711-CF GLP-1 (7-36), [ 125 I]- GLP-1 agonist NEX308 Secretin Gs/Gq Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-712-A AequoZen frozen ready to screen cells CHO-K1 X ES-712-AF 3 mGlu 5A Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-555-A Quisqualic Acid,[ H] mGlu 1, mGlu 5 agonist NET1165 Glutamate Membrane preparation CHO-K1 X ES-555-M400UA

mGlu 7 Gi/G o Human ValiScreen transfected cell line CHO-K1 X ES-553-C cAMPZen frozen ready to screen cells CHO-K1 X ES-553-CF

Glycoprotein Hormone TSH All Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-790-A 6 125 125 6 Gonadotropin-releasing Hormone GnRH Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-600-A Luteinizing Hormone-Releasing Hormone (D-Trp ), [ I]-, ([ I]-(D-Trp )-LHRH) GnRH agonist NEX365 Membrane preparation CHO-K1 X ES-600-M400UA Luteinizing Hormone Releasing Hormone (Tyr 5), [ 125 I]- agonist NEX163

GPR SUCNR1 (Succinate, GPR91) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-744-A AequoZen frozen ready to screen cells CHO-K1 X ES-744-AF

GPR120 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-800-A AequoZen frozen ready to screen cells CHO-K1 X ES-800-AF

OXGR1 (GPR99, Citric acid cycle) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-743-A AequoZen frozen ready to screen cells CHO-K1 X ES-743-AF Growth Hormone-Releasing Factor GRF 1-44 amide, [ 125 I]- agonist NEX426 3 Histamine H1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-390-A Pyrilamine, [pyridinyl 5- H]-, (Mepyramine) H1 antagonist NET594 AequoZen frozen ready to screen cells CHO-K1 X ES-390-AF Histamine dihydrochloride, [ring, methylenes- 3H(N)]- endogenous NET732 ValiScreen transfected cell line CHO-K1 X X ES-390-C Membrane preparation CHO-K1 X ES-390-M400UA

November 2011 Page 6 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Histamine (continued) H2 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-391-A Tiotidine, [methyl- H]-, (ICI 125,211) H2 antagonist NET688 3 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-391-AF Histamine dihydrochloride, [ring, methylenes- H(N)]- endogenous NET732 125 ValiScreen transfected cell line CHO-K1 X X ES-391-C Aminopotentidine, [ I]- H2 antagonist NEX431 cAMPZen frozen ready to screen cells CHO-K1 X ES-391-CF Membrane preparation HEK293 X 6110565400UA CHO-K1 X ES-391-M400UA 3 H3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-392-A Methylhistamine dihydrocloride, N- α-[methyl- H]- H3 agonist NET1027 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-392-AF Proxyfan, [ I]- H3 protean agonist NEX436 ValiScreen transfected cell line CHO-K1 X X X ES-392-C cAMPZen frozen ready to screen cells CHO-K1 X ES-392-CF Membrane preparation CHO-K1 X X ES-392-M400UA 3 H4 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-393-A Histamine dihydrochloride, [ring, methylenes- H(N)]- endogenous NET732

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-393-AF ValiScreen transfected cell line CHO-K1 X X X ES-393-C cAMPZen frozen ready to screen cells CHO-K1 X ES-393-CF Membrane preparation CHO-K1 X X ES-393-M400UA 125 KiSS1 (Metastin) KISS1 (GPR54) Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-630-A Metastin 45-54, [ I]- agonist NEX395 ValiScreen transfected cell line MDA-MB-435S X X ES-630-C Membrane preparation MDA-MB-435S X ES-630-M400UA CHO-K1 X RBHMTSM400UA 3 Leukotriene BLT 1 (LTB4R1) Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-340-A Leukotriene B 4, [5,6,8,9,11,12,14,15- H(N)]- endogenous NET852 ValiScreen transfected cell line CHO-K1 X X X ES-340-C cAMPZen frozen ready to screen cells CHO-K1 X ES-340-CF Membrane preparation CHO-K1 X X ES-340-M400UA 3 CysLT 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-470-A Leukotriene D 4, [14,15,19,20- H(N)]- endogenous NET1019 3 ValiScreen transfected cell line CHO-K1 X X X ES-470-C Leukotriene C 4, [14,15,19,20- H(N)]- endogenous NET1018 Membrane preparation CHO-K1 X ES-470-M400UA

OXE (HM74-like) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X ES-640-A

Lysophospholipid S1P2 (EDG5) Gq Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-594-A AequoZen frozen ready to screen cells CHO-K1 X ES-594-AF

S1P4 (EDG6) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαq/i X X ES-592-A

AequoZen frozen ready to screen cells CHO-K1 Gαq/i X ES-592-AF

S1P5 (EDG8) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαq/i X X ES-593-A

AequoZen frozen ready to screen cells CHO-K1 Gαq/i X ES-593-AF

Mas related MrgB3 Gi/G o Rat AequoScreen aequorin double-transfected cell line CHO-K1 Gαqi/5 X ES-745-A

AequoZen frozen ready to screen cells CHO-K1 Gαqi/5 X ES-745-AF

MrgX1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-740-A

MrgX2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-742-A 125 Melanin-Concentrating Hormone MCH 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-370-A S36057 (Tyr), [ I]- MCH receptor agonist NEX396 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-370-AF Melanin-Concentrating Hormone, [ I]- MCH receptor agonist NEX373 ValiScreen transfected cell line CHO-K1 X X X ES-370-C Melanin-Concentrating Hormone (Phe 13 , Tyr 19 ), [125 I]- MCH receptor agonist NEX375 125 cAMPZen frozen ready to screen cells CHO-K1 X ES-370-CF MCH-1R Antagonist, [ I]- MCH 1 antagonist NEX406 Membrane preparation CHO-K1 X X ES-370-M400UA 125 MCH 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-371-A S36057 (Tyr), [ I]- MCH receptor agonist NEX396 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-371-AF Melanin-Concentrating Hormone, [ I]- MCH receptor agonist NEX373 ValiScreen transfected cell line 1321N1 X X ES-372-C Melanin-Concentrating Hormone (Phe 13 , Tyr 19 ), [125 I]- MCH receptor agonist NEX375 Membrane preparation 1321N1 X ES-372-M400UA 125 MC 1 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-195-C SHU9119, [ I]- MC 1, MC 5 agonist NEX362 cAMPZen frozen ready to screen cells CHO-K1 X ES-195-CF α-MSH (Nle 4, D-Phe 7), [ 125 I]- MC agonist NEX352 Membrane preparation CHO-K1 X ES-195-M400UA 23 125 MC 2 ACTH (1-39) (Tyr ), [ I]- MC 2 agonist NEX165 2 125 ACTH (1-39) (Tyr ), [ I]- MC 2 agonist NEX342 4 7 125 MC 3 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-193-C α-MSH (Nle , D-Phe ), [ I]- MC agonist NEX352 125 cAMPZen frozen ready to screen cells CHO-K1 X ES-193-CF SHU9119, [ I]- MC 3, MC 4 antagonist NEX362 Membrane preparation HEK293 X RBXMC3M400UA CHO-K1 X ES-193-M400UA Mouse ValiScreen transfected cell line CHO-K1 X X ES-190-C cAMPZen frozen ready to screen cells CHO-K1 X ES-190-CF Membrane preparation CHO-K1 X ES-190-M400UA 4 7 125 MC 4 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-191-A α-MSH (Nle , D-Phe ), [ I]- MC agonist NEX352 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-191-AF SHU9119, [ I]- MC 3, MC 4 antagonist NEX362 125 ValiScreen transfected cell line CHO-K1 X X ES-191-C AGRP (Ac 87-132), [ I]- MC 4 agonist NEX372 125 cAMPZen frozen ready to screen cells CHO-K1 X ES-191-CF MT II (His), [ I]- MC 4 agonist NEX374 Membrane preparation HEK293 X RBHMC4M400UA CHO-K1 X ES-191-M400UA

November 2011 Page 7 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 4 7 125 Melanocortin (continued) MC 5 Gs Human ValiScreen transfected cell line CHO-K1 X X ES-194-C α-MSH (Nle , D-Phe ), [ I]- MC agonist NEX352 125 cAMPZen frozen ready to screen cells CHO-K1 X ES-194-CF SHU9119, [ I]- MC 1, MC 5 agonist NEX362 Membrane preparation HEK293 EBNA X RBXMC5M400UA CHO-K1 X ES-194-M400UA Mouse ValiScreen transfected cell line CHO-K1 X X ES-192-C cAMPZen frozen ready to screen cells CHO-K1 X ES-192-CF Membrane preparation CHO-K1 X ES-192-M400UA 125 Melatonin MT 1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X ES-620-A Melatonin, 2-[ I]- agonist NEX236

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-620-AF ValiScreen transfected cell line CHO-K1 X X ES-620-C Membrane preparation CHO-K1 X X ES-620-M400UA 125 MT 2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-621-A Melatonin, 2-[ I]- agonist NEX236 AequoZen frozen ready to screen cells CHO-K1 X X ES-621-AF ValiScreen transfected cell line CHO-K1 X X ES-621-C Membrane preparation CHO-K1 X X ES-621-M400UA 125 Motilin motilin Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-380-A Motilin, [ I]- endogenous NEX378 ValiScreen transfected cell line CHO-K1 X X X ES-380-C Membrane preparation HEK293 X RBHMOTM400UA CHO-K1 X ES-380-M400UA Muscarinic (see Acetylcholine) Neurokinin (see Tachykinin) 18 125 125 Neuromedin U NMU1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-450-A Neuromedin U-25 (Tyr ), [ I]-, ([ I]-NMU-25) NMU1, NMU2 agonist NEX383 ValiScreen transfected cell line CHO-K1 X X X ES-450-C Neuromedin U-8, [ 125 I]- NMU1, NMU2 agonist NEX392 Membrane preparation HEK293 X RBHNU1M400UA CHO-K1 X ES-450-M400UA 125 NMU2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-451-A Neuromedin U-8, [ I]- NMU1, NMU2 agonist NEX392 ValiScreen transfected cell line CHO-K1 X X X ES-451-C Neuromedin U-25 (Tyr 18 ), [ 125 I]-, ([ 125 I]-NMU-25) NMU1, NMU2 agonist NEX383 Membrane preparation HEK293 X RBHNU2M400UA CHO-K1 X ES-451-M400UA 10 125 Neuropeptide S NPS Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-770-A Neuropeptide S (Tyr ), [ I]- endogenous NEX411 ValiScreen transfected cell line CHO-K1 X X X ES-770-C 1 3 125 Neuropeptide FF NPFF1 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X ES-491-C Neuropeptide FF (D-Tyr , N-MePhe ), [ I]- NPFF1, NPFF2 agonist NEX381 cAMPZen frozen ready to screen cells CHO-K1 X ES-491-CF Membrane preparation CHO-DHFR(-) X RBHNF1M400UA CHO-K1 X ES-491-M400UA 1 3 125 NPFF2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαq/i X X X ES-490-A Neuropeptide FF (D-Tyr , N-MePhe ), [ I]- NPFF1, NPFF2 agonist NEX381 ValiScreen transfected cell line CHO-K1 X X X ES-490-C cAMPZen frozen ready to screen cells CHO-K1 X ES-490-CF Membrane preparation CHO-K1 X RBHNF2M400UA 125 Neuropeptide W/B NPBW2 (GPR8) Gi/G o Human Membrane preparation HEK293 X RBHGP8M400UA Neuropeptide W23, [ I]- NPBW2, endogenous NEX403 Neuropeptide B23 (Des-Br), [ 125 I]- NPBW2, endogenous NEX405 125 125 Neuropeptide Y Y1 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-351-A Peptide YY (porcine), [ I]-, ([ I]-PYY) NPY agonist NEX240 31 34 125 ValiScreen transfected cell line CHO-K1 X X ES-351-C Peptide YY (Leu , Pro ), [ I]- Y1 agonist NEX334 cAMPZen frozen ready to screen cells CHO-K1 X ES-351-CF Pancreatic polypeptide, [ 125 I]- endogenous NEX315 Membrane preparation Sf9 X 6110133400UA Peptide YY (human), [ 125 I]-, ([ 125 I]-PYY) endogenous NEX341 Non-recombinant, membrane preparation SK-N-MC X RBHNP1M400UA Neuropeptide Y (porcine), [ 125 I]- agonist NEX222 125 125 Y2 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-352-A Peptide YY (porcine), [ I]-, ([ I]-PYY) NPY agonist NEX240 AequoZen frozen ready to screen cells CHO-K1 X ES-352-AF Pancreatic polypeptide, [ 125 I]- endogenous NEX315 ValiScreen transfected cell line CHO-K1 X X ES-352-C Peptide YY (human), [ 125 I]-, ([ 125 I]-PYY) endogenous NEX341 cAMPZen frozen ready to screen cells CHO-K1 X ES-352-CF Neuropeptide Y (porcine), [ 125 I]- agonist NEX222 Non-recombinant, membrane preparation KAN-TS X RBHNP2M400UA 3 125 Neurotensin NTS 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-690-A Neurotensin (Tyr ), [ I]- endogenous NEX198 AequoZen frozen ready to screen cells CHO-K1 X ES-690-AF Neurotensin, [3,11-tyrosyl-3,5- 3H(N)]- endogenous NET605 ValiScreen transfected cell line CHO-K1 X X ES-690-C Membrane preparation CHO-K1 X 6110518400UA HEK293 X RBXNT1M400UA CHO-K1 X ES-690-M400UA 3 125 NTS 2 Gq/G 11 Human ValiScreen transfected cell line 1321N1 X ES-691-C Neurotensin (Tyr ), [ I]- endogenous NEX198 Membrane preparation HEK293 X 6110566400UA Neurotensin, [3,11-tyrosyl-3,5- 3H(N)]- endogenous NET605 1321N1 X ES-691-M400UA

Nicotinic GPR109A (HM74A) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gαq/i X X X ES-760-A ValiScreen transfected cell line CHO-K1 X X X ES-760-C cAMPZen frozen ready to screen cells CHO-K1 X ES-760-CF Membrane preparation CHO-K1 X X ES-760-M400UA IMR32 X RBHNICM400UA Obestatin Obestatin, [ 125 I]- endogenous NEX415 3 Opioid delta Gi/G o Human Membrane preparation CHO-K1 X RBHODM400UA Naltrindole, [5',7'- H]- delta antagonist NET1065 HEK293 X 6110549400UA Met (O) 5-Enkephalin, [ 125 I-Tyr 1]- delta agonist NEX149 Mouse Membrane preparation Sf9 X 6110115400UA Enkephalin (2-D-Alanine-5-D-Leucine), [Tyrosyl-3,5-3H(N)]- delta agonist NET648 Enkephalin, [Tyrosyl-2,6- 3H(N)]-, (2-D-Penicillamine, 5-D-Penicillamine) (DPDPE) delta agonist NET922 Deltorphin II (2-D-Ala), [tyrosyl-3,5- 3H]- delta agonist NET1087 Diprenorphine [15,16- 3H]- antagonist NET1121

November 2011 Page 8 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Opioid (continued) kappa Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-541-A Diprenorphine, [15,16- H]- antagonist NET1121 ValiScreen transfected cell line CHO-K1 X X X ES-541-C U-69,593, [phenyl-3,4- 3H]- kappa agonist NET952 Membrane preparation HEK293 X 6110558400UA CHO-K1 X X ES-541-M400UA 3 mu Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-542-A Diprenorphine, [15,16- H]- antagonist NET1121 3 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-542-AF Naloxone, [N-allyl-2,3- H]- mu antagonist NET719 ValiScreen transfected cell line CHO-K1 X X X ES-542-C DAMGO, [tyrosyl-3,5- 3H(N)]- mu agonist NET902 cAMPZen frozen ready to screen cells CHO-K1 X ES-542-CF Membrane preparation CHO-K1 X X ES-542-M400UA 14 125 NOP (ORL1) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-230-A Nociceptin (Tyr ), [ I]- NOP agonist NEX338 ValiScreen transfected cell line CHO-K1 X X X ES-230-C Nociceptin, [leucyl-3,4,5- 3H]- NOP agonist NET1130 cAMPZen frozen ready to screen cells CHO-K1 X ES-230-CF Membrane preparation CHO-K1 X 6110540400UA HEK293 X RBHORLM400UA 125 Orexin OX 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-330-A Orexin-A, [ I]- OX 1, OX 2 agonist NEX367 AequoZen frozen ready to screen cells CHO-K1 X ES-330-AF ValiScreen transfected cell line CHO-K1 X X ES-330-C Membrane preparation CHO-K1 X ES-330-M400UA 125 OX 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-331-A Orexin-A, [ I]- OX 1, OX 2 agonist NEX367

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-331-AF ValiScreen transfected cell line CHO-K1 X X ES-331-C Ornithine Vasotocin Analog, [ 125 I]- antagonist NEX254 Oxytocin (Tyr 2), [ 125 I] - endogenous NEX187 Oxytocin, [Tyr 2,6- 3H]-, endogenous NET858 8,21 34 125 Parathyroid Hormone Parathyroid Hormone (1-34) amide (Nle ,Tyr ) (Rat), [ I]- PTH 1, PTH 2 agonist NEX353 Parathyroid Hormone (1-34) (Nle 8,18 , Tyr 34 ) (Human), [ 125 I]- endogenous NEX397 125 Peptide P518 QRFP (OX 2-like, GPR103) Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-670-A QRFP-43, [ I]- QRFP, endogenous NEX408 ValiScreen transfected cell line CHO-K1 X X ES-670-C Membrane preparation CHO-K1 X ES-670-M400UA HEK293 X RBH103M400UA Platelet Activating Factor Hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, 1-O-[acetyl- 3H(N)]- endogenous NET910 125 Prokineticin PKR 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-750-A MIT-1, [ I]-, Bolton-Hunter labeled PKR 1, PKR 2 antagonist NEX410

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-750-AF

Membrane preparation CHO-K1 Gα16 X ES-750-M400UA 125 PKR 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-751-A MIT-1, [ I]-, Bolton-Hunter labeled PKR 1, PKR 2 antagonist NEX410 AequoZen frozen ready to screen cells CHO-K1 X ES-751-AF Membrane preparation CHO-K1 X ES-751-M400UA HEK293 X RBHPK2M400UA ValiScreen transfected cell line CHO-K1 X ES-751-C cAMPZen frozen ready to screen cells CHO-K1 X ES-751-CF 20 125 Prolactin Releasing Peptide PRRP Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-480-A Prolactin-Releasing Peptide (Tyr ), [ I]- PRRP agonist NEX384 ValiScreen transfected cell line CHO-K1 X X ES-480-C Membrane preparation CHO-K1 X ES-480-M400UA 3 Prostanoid DP 1 Gs Human AequoScreen aequorin double-transfected cell line 1321N1 X X X ES-560-A Prostaglandin D 2, [5,6,8,9,12,14,15- H(N)]- endogenous NET616 ValiScreen transfected cell line 1321N1 X X ES-560-C cAMPZen frozen ready to screen cells 1321N1 X ES-560-CF Membrane preparation 1321N1 X ES-560-M400UA 3 DP 2 (CRTH2) Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-561-A Prostaglandin D 2, [5,6,8,9,12,14,15- H(N)]- endogenous NET616 3 ValiScreen transfected cell line CHO-K1 X X X ES-561-C Prostaglandin E 2, [5,6,8,11,12,14,15- H(N)]- endogenous NET428 cAMPZen frozen ready to screen cells CHO-K1 X ES-561-CF Membrane preparation HEK293 X RBHTH2M400UA CHO-K1 X X ES-561-M400UA 3 EP 2 Gs Human ValiScreen transfected cell line HEK293 X X ES-562-C Prostaglandin E 2, [5,6,8,11,12,14,15- H(N)]- endogenous NET428 cAMPZen frozen ready to screen cells HEK293 X ES-562-CF Membrane preparation HEK293 X ES-562-M400UA 3 EP 4 Gs Human AequoScreen aequorin double-transfected cell line HEK293 Gα16 X X X ES-563-A Prostaglandin E 2, [5,6,8,11,12,14,15- H(N)]- endogenous NET428 ValiScreen transfected cell line HEK293 X X ES-563-C Membrane preparation HEK293 X ES-563-M400UA 3 FP Gq/G 11 Human ValiScreen transfected cell line 1321N1 X X ES-564-C Prostaglandin F 2α, [5,6,8,9,11,12,14,15- H(N)] endogenous NET433 Membrane preparation 1321N1 X ES-564-M400UA 3 TP Gq/G 11 Human ValiScreen transfected cell line HEK293 EBNA X RBHTPC SQ 29548, [5,6- H]- TP NET936 Membrane preparation HEK293 EBNA X RBHTPM400UA

Protease-activated PAR2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-780-A

PAR4 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-782-A AequoZen frozen ready to screen cells CHO-K1 X ES-782-AF

Purinergic P2Y P2Y 2 Gq/G 11 Human ValiScreen transfected cell line 1321N1 X ES-290-C

P2Y 6 Gq/G 11 Human ValiScreen transfected cell line 1321N1 X ES-292-C

P2Y 11 Gq/G 11 Human AequoScreen aequorin double-transfected cell line 1321N1 X ES-293-A AequoZen frozen ready to screen cells 1321N1 X ES-293-AF

P2Y 12 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X RBHP12C

November 2011 Page 9 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 125 Relaxin RXFP3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 X ES-656-A INSL-3, [ I]- RXFP2, RXFP3 agonist NEX419 ValiScreen transfected cell line CHO-K1 X X ES-656-C cAMPZen frozen ready to screen cells CHO-K1 X ES-656-CF Membrane preparation CHO-K1 X ES-656-M400UA 125 RXFP4 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X ES-659-C INSL-3, [ I]- RXFP2, RXFP3 agonist NEX419 Membrane preparation CHO-K1 X ES-659-M400UA AequoScreen aequorin double-transfected cell line CHO-K1 X ES-659-A RXFP1 & RXFP2 H2 Relaxin, [ 125 I]-, Bolton-Hunter Labeled RXFP1 (LGR7) agonist NEX409 H3 Relaxin, [ 125 I]- RXFP1 (LGR7) agonist NEX418 (see 5-hydroxytryptamine) 11 125 Somatostatin sst 1 Gi/G o Human ValiScreen transfected cell line CHO-K1 X X ES-520-C Somatostatin (Tyr ), [ I]- sst 1-sst 5 agonist NEX389 11 125 Membrane preparation CHO-K1 X X ES-520-M400UA Somatostatin-14 (Tyr ) (human, recombinant), [ I] sst 1-sst 5 agonist NEX446 11 125 sst 2a Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-521-A Somatostatin (Tyr ), [ I]- sst 1-sst 5 agonist NEX389

AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-521-AF 11 125 ValiScreen transfected cell line CHO-K1 X X X ES-521-C Somatostatin-14 (Tyr ) (human, recombinant), [ I] sst 1-sst 5 agonist NEX446 cAMPZen frozen ready to screen cells CHO-K1 X ES-521-CF Membrane preparation CHO-K1 X X ES-521-M400UA 11 125 sst 3 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-523-A Somatostatin (Tyr ), [ I]- sst 1-sst 5 agonist NEX389 11 125 ValiScreen transfected cell line CHO-K1 X X X ES-523-C Somatostatin-14 (Tyr ) (human, recombinant), [ I] sst 1-sst 5 agonist NEX446 cAMPZen frozen ready to screen cells CHO-K1 X ES-523-CF Membrane preparation CHO-K1 X X ES-523-M400UA 11 125 sst 4 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-524-A Somatostatin (Tyr ), [ I]- sst 1-sst 5 agonist NEX389 11 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-524-AF Somatostatin-14 (Tyr ) (human, recombinant), [ I] sst 1-sst 5 agonist NEX446 ValiScreen transfected cell line CHO-K1 X X X ES-524-C cAMPZen frozen ready to screen cells CHO-K1 X ES-524-CF Membrane preparation HEK293 EBNA X RBHST4M400UA CHO-K1 X X ES-524-M400UA 11 125 sst 5 Gi/G o Human AequoScreen aequorin double-transfected cell line CHO-K1 Gα16 X X X ES-522-A Somatostatin (Tyr ), [ I]- sst 1-sst 5 agonist NEX389 11 125 AequoZen frozen ready to screen cells CHO-K1 Gα16 X ES-522-AF Somatostatin-14 (Tyr ) (human, recombinant), [ I] sst 1-sst 5 agonist NEX446 ValiScreen transfected cell line CHO-K1 X X X ES-522-C cAMPZen frozen ready to screen cells CHO-K1 X ES-522-CF Membrane preparation HEK293 EBNA X RBHST5M400UA CHO-K1 X X ES-522-M400UA Succinate (see GPR91) 3 Tachykinin NK 1 Gq/G 11 Human Non-recombinant, membrane preparation UC11 X 6110551400UA Substance P (Lys ), [125I]-, Bolton-Hunter labeled NK 1 agonist NEX190 8 125 Substance P (Tyr ), [ I]- NK 1 agonist NEX152 9 3 Substance-P (Sar , 11-Met(O 2)), [2-prolyl-3,4- H]- NK 1 agonist NET1025 125 Hemokinin-1, [ I]-, Bolton-Hunter labeled NK 1 agonist NEX414 3 Substance P, [leucyl-3,4,5- H(N)]- NK 1 agonist NET1115

Substance-P (9-Sar, 11-Met(O2)), [2-Prolyl-3, 4-3H]- NK 1 agonist NET1025 3 NK 2 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-251-A SR 48968, [benzamide-4- H]- NK 2 antagonist NET1077 4 125 ValiScreen transfected cell line CHO-K1 X X ES-251-C Eledoisin (Lys ), [ I]-, Bolton-Hunter labeled NK 2, NK 3 agonist NEX218 125 125 125 Membrane preparation CHO-K1 X ES-251-M400UA Neurokinin A, [ I]-, ([ I]-Substance K, [ I]-NKA) NK 2 agonist NEX252 4 125 NK 3 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-252-A Eledoisin (Lys ), [ I]-, Bolton-Hunter labeled NK 2, NK 3 agonist NEX218 7 125 125 ValiScreen transfected cell line CHO-K1 X X ES-252-C Neurokinin B (MePhe ), [ I]-, ([ I]-NKB) NK 3 agonist NEX285 3 Membrane preparation CHO-K1 X ES-252-M400UA Senktide, [phenylalanyl-3,4,5- H]- NK 3 agonist NET997 3 SR142801, [ H] NK 3 agonist NET1183 2 3 Thyrotropin Releasing Hormone TRH 1 Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-700-A Thyrotropin Releasing Hormone (3-Methyl-histidine ), [L-histidyl-4- H(N)]- TRH agonist NET705 AequoZen frozen ready to screen cells CHO-K1 X ES-700-AF Thyrotropin Releasing Hormone (His 2), [ 125 I]- TRH agonist NEX153 ValiScreen transfected cell line CHO-K1 X X ES-700-C Membrane preparation CHO-K1 X ES-700-M400UA 125 9 Urotensin UT (Uro II, GPR14) Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-440-A Urotensin II (human), [ I-Tyr ]- endogenous NEX379 ValiScreen transfected cell line CHO-K1 X X ES-440-C PhotoScreen Photina double-transfected cell line CHO-K1 X AX-007-PCF Membrane preparation CHO-K1 X ES-440-M400UA Rat AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-441-A AequoZen frozen ready to screen cells CHO-K1 X ES-441-AF Mouse Membrane preparation CHO-K1 X RBMUR2M400UA 125 Vasoactive Intestinal Peptide PAC 1 (PACAP) Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-272-A PACAP 27, [ I]- PAC 1, endogenous NEX294 125 VPAC 1 (VIP) Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-273-A Vasoactive Intestinal Polypeptide, [ I]- VPAC 1, endogenous NEX192 Non-recombinant, membrane preparation HT29 X RBHVIPM400UA 125 VPAC 2 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 X X ES-274-A Vasoactive Intestinal Polypeptide, [ I]- 1 2 125 6 Vasopressin V1A Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-361-A Vasopressin (linear), V 1A antagonist (Phenylacetyl , 0-Me-D-Tyr , [ I-Arg ]-) V1A antagonist NEX310 AequoZen frozen ready to screen cells CHO-K1 X ES-361-AF Vasopressin, 8-arginine, [phenylalanyl-3,4,5- 3H(N)]- endogenous NET800 ValiScreen transfected cell line 1321N1 X X ES-361-C Membrane preparation HEK293 X RBHV1AM400UA 1321N1 X ES-361-M400UA

November 2011 Page 10 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for 3 Vasopressin (continued) V1B Gq/G 11 Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-362-A Vasopressin, 8-arginine, [phenylalanyl-3,4,5- H(N)]- endogenous NET800 AequoZen frozen ready to screen cells CHO-K1 X ES-362-AF ValiScreen transfected cell line 1321N1 X X ES-362-C Membrane preparation CHO-K1 X 6110543400UA HEK293 X RBHV1BM400UA 1321N1 X ES-362-M400UA Rat ValiScreen transfected cell line CHO-K1 X X ES-360-C Membrane preparation CHO-K1 X ES-360-M400UA 3 V2 Gs Human AequoScreen aequorin double-transfected cell line CHO-K1 X X X ES-363-A Vasopressin, 8-arginine, [phenylalanyl-3,4,5- H(N)]- endogenous NET800 ValiScreen transfected cell line 1321N1 X X ES-363-C cAMPZen frozen ready to screen cells 1321N1 X ES-363-CF Membrane preparation CHO-K1 X 6110541400UA 1321N1 X ES-363-M400UA Cytokine & Growth Hormone Receptors Growth hormone Please inquire for receptor availability Growth hormone, [ 125 I]- endogenous NEX100 Interleukin Please inquire for receptor availability Interleukin-1 β, [ 125 I]- endogenous NEX232 Interleukin-2, [ 125 I]- endogenous NEX229 Interleukin-6, [ 125 I]- endogenous NEX269 Leptin Please inquire for receptor availability Leptin, [ 125 I]- endogenous NEX347 Leptin (murine), [ 125 I]- ObR agonist NEX340 Prolactin Please inquire for receptor availability Prolactin, [ 125 I] endogenous NEX108 Natriuretic Peptide Receptors 125 Atrial Natriuretic Factor Please inquire for receptor availability ANF (rat), [ I]- endogenous NEX228 ANF (human), [ 125 I]- endogenous NEX425 Kinase Receptors 125 Epidermal Growth Factor EGF Human Non-recombinant, membrane preparation A431 X RBHEGFM400UA Epidermal Growth Factor (murine), [ I]- endogenous NEX160 125 EGFR1 Human AequoScreen aequorin double-transfected cell line CHO-K1 X upon request Epidermal Growth Factor (human), [ I]- endogenous NEX428 125 Fibroblast Growth Factor FGFR1 Human AequoScreen aequorin double-transfected cell line CHO-K1 X upon request Fibroblast Growth Factor basic, [ I]- endogenous NEX268 Granulocyte Macrophage-Colony Stimulating- Please inquire for receptor availability Granulocyte Macrophage-Colony Stimulating-Factor- [125 I]- endogenous NEX249 Factor Insulin-like Growth Factor Please inquire for receptor availability Insulin-like Growth Factor I, [ 125 I]- endogenous NEX241 Insulin-like Growth Factor II, [ 125 I]- endogenous NEX429 Nerve-Growth factor Please inquire for receptor availability Nerve Growth Factor (human), [ 125 I]- endogenous NEX325 Nerve Growth Factor (human), recombinant, [ 125 I]- endogenous NEX440 Nerve Growth Factor (rat) endogenous NEX444 Platelet-Derived Growth Factor Please inquire for receptor availability PDGF-BB, [ 125 I]- endogenous NEX433 Transforming Growth Factor Please inquire for receptor availability Transforming Growth Factor β1, [ 125 I]- endogenous NEX267 Tumor Necrosis Factor Please inquire for receptor availability Tumor Necrosis Factor- α, [ 125 I]- endogenous NEX257 Vascular Endothelial Growth factor Please inquire for receptor availability Vascular Endothelial Growth factor, [ 125 I]- endogenous NEX328 Insulin Please inquire for receptor availability Insulin (porcine), [ 125 I]- endogenous NEX104 Insulin (human), [ 125 I]- endogenous NEX420 Insulin (Tyr A14 ) (porcine), [ 125 I]- endogenous NEX196 Transporters Acetylcholine Hemicholinium-3 diacetate salt, [methyl- 3H]- Uptake inhibitor NET884 3 Dopamine n.a. Human Membrane preparation CHO-K1 X RBHDATM400UA WIN 35428, [N-methyl- H]- Uptake inhibitor NET1033 RTI-55, [ 125 I]- Uptake inhibitor NEX272 RTI-121, [ 125 I]- Uptake inhibitor NEX318 , levo-[benzoyl-3,4- 3H(N)]- Uptake inhibitor NET510 , [4'- 3H]- Uptake inhibitor NET816 GBR 12935, [propylene-2,3- 3H]- Uptake inhibitor NET918 Methyl-4-phenylpyridinium acetate, [N-methyl- 3H]- , (MPP(+)) Uptake inhibitor NET914 3 Norepinephrine n.a. Human Membrane preparation MDCK X RBHNETM400UA hydrochloride, [N-methyl- H]- Uptake inhibitor NET1084 Desmethylimipramine hydrochloride, [benzene ring, 10,11- 3H]- Uptake inhibitor NET593 3 Serotonin n.a. Human Membrane preparation HEK293 X RBHSTM400UA hydrochloride, [benzene ring- H(N)]- Uptake inhibitor NET576 Paroxetine, [phenyl-6'- 3H]- Uptake inhibitor NET869 Citalopram, [N-methyl-3H]- Uptake inhibitor NET1039 Transferrin Transferrin, [ 125 I]- endogenous NEX212 Ion Channels

5-Hydroxytryptamine 5-HT 3A AequoScreen aequorin double-transfected cell line HEK293 X ES-402-A AequoZen frozen ready to screen cells HEK293 X ES-402-AF 3 5-HT 3 Human Membrane preparation HEK293 X RBHS3M400UA GR 65630, [N-methyl- H]- 5-HT 3 blocker NET1011 3 BRL-43694, [9-methyl- H]- 5-HT 3 blocker NET1030

November 2011 Page 11 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for Aspartate Please inquire for receptor/channel availability Aspartic acid, D-[2,3- 3H]- endogenous NET581 Benzodiazepine (GABA-A) Please inquire for receptor/channel availability Diazepam, [methyl- 3H]- GABA-A agonist NET564 Flunitrazepam, [methyl- 3H]- GABA-A agonist NET567 Muscimol, 3-hydroxy-5-aminomethylisoxazole, [methylene- 3H(N)]- GABA-A agonist NET574 SR 95531, [butyryl-2,3- 3H]- GABA-A agonist NET946 Pregnan-3 α-ol-20-one, 5 α- [9,11,12- 3H(N)]- GABA-A modulator NET1047 RO 5-4864, [N-methyl- 3H]- peripheral antagonist NET704 PK 11195, [N-methyl- 3H]- peripheral agonist NET885 RO 15-1788, [N-methyl- 3H]- central antagonist NET757 RO 15-4513, [7,9- 3H]- photoaffinity antagonist NET925 Calcium Channel Please inquire for receptor/channel availability Calcium-45 NEZ013 ω-Conotoxin MVIIC, [ 125 I]- Non-L-type anatgonist NEX323 ω-Conotoxin GVIA (Tyr 22 ), [ 125 I]- N-type antagonist NEX239 Diltiazem, cis-(+)-[N-methyl- 3H]- L-type antagonist NET847 Nitrendipine, [5-methyl- 3H]- L-type antagonist NET741 PN200-110, (+)-[5-methyl- 3H]- L-type antagonist NET863 Verapamil hydrochloride, [N-methyl- 3H]- L-type antagonist NET810 Ryanodine, [9,21- 3H(N)]- Ryanodine agonist NET950 CRAC (Stim1 + Orai1) PhotoScreen Photina double-transfected cell line HEK293 Calcium Release Activated X AX-013-PCL validated in e-phys Glutamate Please inquire for receptor/channel availability CGP 39653, [propyl-2,3, 3H]- NMDA agonist NET1050 MK-801, (+)-[3- 3H]- non-competitive NMDA agonist NET972 Ifenprodil, [phenyl- 3H]- NMDA; polyamine site NET1089 Amino-3-hydroxy-5-methylisoxazole-4-propionic acid, DL- α-[5-methyl- 3H]- AMPA agonist NET833 Kainic acid, [vinylidene- 3H]- Kainate agonist NET875 TCP, N-(1-(2 Thienyl)cyclohexyl)-3,4-, [piperidyl-3,4- 3H(N)]- Non-competitive glutamate antagonist NET886 NAAG, [glutamate-3,4- 3H]- endogenous NET1082 Glutamic Acid, L-[3,4- 3H]- endogenous NET490 Glycine Please inquire for receptor/channel availability Serine, D-[ 3H(G)]- endogenous NET1092 , [benzene ring- 3H]- antagonist NET490 + 11 125 hERG K channel Human Membrane preparation HEK293 X RBHERGM400UA BeKm-1 (Tyr ), [ I]- ERG blocker NEX412 Astemizole, [O-methyl- 3H]- ERG blocker NET1140 Dofetilide, [N-methyl- 3H] ERG blocker NET1144 18 125 Potassium Channel Please inquire for receptor/channel availability Apamin (His -NH 2), [ I]- antagonist NET242 Glibenclamide, Glyburide, [cyclohexyl-2,3- 3H(N)]- ATP-dependent antagonist NET1024 α-Dendrotoxin, [ 125 I]- blocker NEX434 Rubidium-86 Radionuclide NEZ072 125 125 Nicotinic n.a. Human Non-recombinant, membrane preparation IMR32 X RBHNICM400UA , [ I]-, ([ I]-IPH) CNS, skeletal muscle and autonomic ganglion activatorNEX358 (+/-)-Epibatidine, [5,6-bicycloheptyl- 3H]- CNS, skeletal muscle and autonomic ganglion activatorNET1102 Choline chloride, [methyl- 3H]- NET109 A-85380, [ 125 I]- Neuronal CNS alpha4 beta2 NEX413 α-Bungarotoxin (Tyr 54 ), [ 125 I]- Neuromuscular blocker NEX126 hydrochloride, [3,5-3H(N)]- CNS activator NET1054 , L-(-)-[N-ethyl- 3H]- CNS activator NET827 Human/ PhotoScreen Photina double-transfected cell line

Purinergic P2RX 2/ p2rx 3 rat validated in e-phys CHO-K1 X AX-014-PCL PhotoScreen Photina double-transfected cell line

P2RX 1 Human validated in e-phys CHO-K1 X AX-009-PCF PhotoScreen Photina double-transfected cell line

P2RX 4 Human validated in e-phys HEK293 X AX-015-PCF Sodium Channel Please inquire for receptor/channel availability Batrachotoxinin A 20- α-Benzoate, [benzoyl-2,5- 3H]- activator NET876 Sodium-22 Radionuclide NEZ081 PhotoScreen Photina double-transfected cell line Transient receptor potential TRPA1 Human validated in e-phys HEK293 X AX-004-PCL ValiScreen transfected cell line TRPC3 Human validated in e-phys and membrane potential assay HEK293 AX-011-C ValiScreen transfected cell line TRPC6 Human validated in e-phys and membrane potential assay HEK293 AX-012-C Vanilloid Resiniferatoxin, [ 125 I]- agonist NEX394 Resiniferatoxin, [ 3H]- agonist NEX1132 ValiScreen transfected cell line

Voltage gated potassium channel Kv1.3 (KCNA3) Human validated in e-phys and membrane potential assay CHO-DUKX AX-010-C Nuclear Receptors Aldosterone Please inquire for receptor availability Aldosterone, D-[1,2,6,7- 3H(N)]- MR, endogenous NET419 Please inquire for receptor availability Dihydrotestosterone (5 α androstan-17 β-ol-3-one), [1,2,4,5,6,7- 3H(N)]- AR, endogenous NET453 , [1, 2, 6, 7, 16, 17- 3H(N)]- AR, endogenous NET553 Testosterone, [1,2,6,7- 3H(N)]- AR, endogenous NET370 Methyltrienolone (R1881) AR agonist NLP005 Methyltrienolone, [17 α-methyl- 3H]- AR agonist NET590 Mibolerone AR agonist NLP024 Mibolerone, [17 α-methyl- 3H]- AR agonist NET919

November 2011 Page 12 Cell Line Frozen Membrane Selector Guide v19 e t a e h e c c Promis- p n g s n m e m e n S P o c G-protein cuous i γγ u u c h i s i Receptor type Subtype Species Product Type Host cell d P M c c s Cat. No. Complementary radioligands Pharmacological action Cat. No. P l e l e coupling G-Protein in T A n r c l a i a G o o B t C C Added i m u u l s F o L In Validated for Ecdysteroid Please inquire for receptor availability Ecdysone, α-[23,24- 3H(N)]- Ecdysteroid agonist NET621 Ponasterone A, [24,25,26,27- 3H(N)]- Ecdysteroid agonist NET1070 Please inquire for receptor availability , [2,4,6,7,16,17- 3H(N)]- ER, endogenous NET517 Estradiol, [2,4,6,7- 3H(N)]- ER, endogenous NET317 Estradiol 17 β-D-glucuronide, [Estradiol-6,7- 3H(N)]- ER, endogenous NET1106 Estradiol, [16 α-[ 125 I]iodo-3,17 β]- ER, endogenous NEX144 Estradiol, [16 α-[ 125 I]iodo-3,17 β]- ER, endogenous NEX144L Estradiol, [ 125 I]- ER, endogenous NEX438 Glucocorticoid Please inquire for receptor availability Dexamethasone, [6,7- 3H(N)]- GR agonist NET467 Cortisol, [ 125 I]- GR, endogenous NEX435 Corticosterone, [1,2,6,7- 3H(N)]- GR, endogenous NET399 Hydrocortisone [1,2,6,7- 3H(N)]-, (Cortisol, [1,2,6,7- 3H(N)]-) GR, endogenous NET396 Progestin Please inquire for receptor availability , [1,2,6,7- 3H(N)]- PR, endogenous NET381 Promegestone (R5020*), [17 α-methyl- 3H]- photoaffinity agonist NET555 Promegestone (R5020*) Solid photoaffinity agonist NLP004 Thyroid hormones Please inquire for receptor availability Thyroxine, L-[ 125 I]-, ([ 125 I]-T4) THR, endogenous NEX111 Thyroxine, L-[ 125 I]-, ([ 125 I]-T4) THR, endogenous NEX111H Thyroxine, L-[ 125 I]-, ([ 125 I]-T4) THR, endogenous NEX111X Triiodothyronine, ([ 125 I]-Reverse T3), L-3,3',5'-[ 125 I]- THR, endogenous NEX109 Triiodothyronine, ([ 125 I]-Reverse T3), L-3,5',3'-[ 125 I]- THR, endogenous NEX110 Triiodothyronine, ([ 125 I]-Reverse T3), L-3,5',3'-[ 125 I]- THR, endogenous NEX110H Triiodothyronine, ([ 125 I]-Reverse T3), L-3,5',3'-[ 125 I]- THR, endogenous NEX110X 3 Vitamin D 3 Please inquire for receptor availability Dihydroxyvitamin D 3, 1 α, 25-[26,27- H]- VDR, endogenous NET626 3 Hydroxyvitamin D 3, 25-[26,27- H]- endogenous NET349 Wild-type membranes 1321N1 cells Wild Type Control Membranes Human Membrane preparation 1321N1 RBH1321M010MG A9 cells Wild Type Control Membranes Mouse Membrane preparation A9 RBMA9M010MG CHO-K1 cells Wild Type Control Membranes Hamster Membrane preparation CHO-K1 RBCCHOM010MG HEK293 cells Wild Type Control Membranes Human Membrane preparation HEK293 RBHHEKM010MG K562 cells Wild Type Control Membranes Human Membrane preparation K562 RBHK56M010MG THP-1 cells Wild Type Control Membranes Human Membrane preparation THP-1 RBHTHPM010MG U373 cells Wild Type Control Membranes Human Membrane preparation U373 RBHU37M010MG Signal transduction Inositol triphosphate Inositol-1,3,4,5-tetrakisphosphate, [inositol-1- 3H(N)]- endogenous NET941 Inositol-1,4,5-trisphosphate, D-[inositol-1- 3H(N)]- endogenous NET911 Nitric oxide Arginine monohydrochloride L-[2,3,4- 3H]- NOS substrate NET1123 3 Phospholipase Phosphatidylinositol 4,5-bisphosphate (PIP 2), [inositol-2- H(N)]- PLC, endogenous NET895 Phosphatidylinositol-4,5-bisphosphate, [inositol-1-3H]- PLC, endogenous NET1135 Sphingomyelinase Sphingomyelin (egg), [choline methyl- 3H]- Sphingomyelenase substrate NET1134 Fatty acid Myristic acid (tetradecanoic acid), [9,10- 3H(N)]- Endogenous substrate NET830 GTP analogue GTPgammaS Non-hydrolyzable GTP analogue NEG030 Parental cell lines

AequoScreen aequorin parental cell line (medium level) CHO-K1 Gα16 X ES-000-A2

AequoZen parental Frozen Cells (medium level) CHO-K1 Gα16 X ES-000-A2F AequoScreen aequorin parental cell line CHO-K1 X ES-000-A12 AequoZen parental Frozen Cells CHO-K1 X ES-000-A12F

AequoScreen aequorin parental cell line CHO-K1 Gαq/i5 X ES-000-A21

AequoZen parental Frozen Cells CHO-K1 Gαq/i5 X ES-000-A21F

AequoScreen aequorin parental cell line CHO-K1 Gα16 X ES-000-A24

AequoZen parental Frozen Cells CHO-K1 Gα16 X ES-000-A24F AequoScreen aequorin parental cell line 1321N1 X ES-000-A27 AequoZen parental Frozen Cells 1321N1 X ES-000-A27F

AequoScreen aequorin parental cell line 1321N1 Gα16 X ES-000-A28

AequoZen parental Frozen Cells 1321N1 Gα16 X ES-000-A28F AequoScreen aequorin parental cell line HEK293 X ES-000-A30 AequoZen parental Frozen Cells HEK293 X ES-000-A30F

AequoScreen aequorin parental cell line HEK293 Gα16 X ES-000-A26

AequoZen parental Frozen Cells HEK293 Gα16 X ES-000-A26F Plasmids AequoScreen aequorin plasmid X ES-002-AC

AequoScreen aequorin plasmid Gα16 X ES-003-AC Starter Kits

Muscarinic and Histamine M5 and H 1 AequoZen frozen cells Starter Kit CHO-K1 X ES-001-AF

Muscarinic and Histamine M5 and H 1 AequoScreen Starter Kit CHO-K1 X ES-001-A Adenosin A3 PhotoScreen Starter Kit CHO-K2 X AX-001-PCF

©2008 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer Inc. AequoScreen, AequoZen, cAMPZen and NEN are registered trademarks of PerkinElmer Inc. PhotoScreen and ValiScreen are trademarks of PerkinElmer Inc. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at anytime without notice and disclaims liability for editorial, pictorial or typographical errors.

PerkinElmer, Inc. www.perkinelmer.com 940 Winter Street Waltham, MA 02451

(800) 762-4000 (+1) 203-925-4602 (THE END)

November 2011 Page 13 Cell Line Frozen Membrane Selector Guide v19