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Pdfs/ Ommended That Initial Cultures Focus on Common Pathogens, Pscmanual/9Pscssicurrent.Pdf)
Clinical Infectious Diseases IDSA GUIDELINE A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for Microbiologya J. Michael Miller,1 Matthew J. Binnicker,2 Sheldon Campbell,3 Karen C. Carroll,4 Kimberle C. Chapin,5 Peter H. Gilligan,6 Mark D. Gonzalez,7 Robert C. Jerris,7 Sue C. Kehl,8 Robin Patel,2 Bobbi S. Pritt,2 Sandra S. Richter,9 Barbara Robinson-Dunn,10 Joseph D. Schwartzman,11 James W. Snyder,12 Sam Telford III,13 Elitza S. Theel,2 Richard B. Thomson Jr,14 Melvin P. Weinstein,15 and Joseph D. Yao2 1Microbiology Technical Services, LLC, Dunwoody, Georgia; 2Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota; 3Yale University School of Medicine, New Haven, Connecticut; 4Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland; 5Department of Pathology, Rhode Island Hospital, Providence; 6Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill; 7Department of Pathology, Children’s Healthcare of Atlanta, Georgia; 8Medical College of Wisconsin, Milwaukee; 9Department of Laboratory Medicine, Cleveland Clinic, Ohio; 10Department of Pathology and Laboratory Medicine, Beaumont Health, Royal Oak, Michigan; 11Dartmouth- Hitchcock Medical Center, Lebanon, New Hampshire; 12Department of Pathology and Laboratory Medicine, University of Louisville, Kentucky; 13Department of Infectious Disease and Global Health, Tufts University, North Grafton, Massachusetts; 14Department of Pathology and Laboratory Medicine, NorthShore University HealthSystem, Evanston, Illinois; and 15Departments of Medicine and Pathology & Laboratory Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey Contents Introduction and Executive Summary I. -
Principle of Infection
23/09/56 Principle of Infection La-or Chompuk, M.D. Department of pathology Faculty of Medicine Infection • Definition: Invasion and multiplication of microorganisms in body tissues • No symptom, local cellular injury, localized symptom, dissemination • Mechanism; competitive metabolism, toxins, intracellular replication, immune response 1 23/09/56 Classification of infectious agents: - classification according to structure - classification according to pathogenesis - classification according to site of multiplication Classification according to structure - Prion - Fungi - Viruses - Protozoa, metazoa - Bacteria - Ectoparasite - Rickettsia, chlamydia, mycoplasma 2 23/09/56 Classification according to pathogenesis • Pathogenic agents; - Virulence: the degree of pathogenicity of a microorganism - Indicated by the severity of disease, the ability to invade tissue - high virulence - low virulence • Opportunistic infection Classification according to site of multiplication - obligate intracellular organisms; Prions, viruses, rickettsiae, chlamydia, some protozoa - facultative intracellular organism; Mycobacteria, Actinomyces, Pseudomonas spp. - extracellular organisms; mycoplasma, fungi, bacteria, metazoa 3 23/09/56 Pathogenesis of Infectious Disease -Host - Pathogen; organism or parasite that cause disease Host factors: 1. General factors; socioeconomic status, behavior pattern, occupational, and internal factors 2. Natural defense mechanism; skin and normal flora, respiratory tract and mucociliary mechanism, Hcl production in stomach, or -
Herpes Simplex Virus-Associated Dermatitis with Either High Or Normal Ige Responded Well to Antiviral Therapy: a Study of 787 Quick-Tzanck-Test-Positive Patients
Article ID: WMC004846 ISSN 2046-1690 Herpes Simplex Virus-Associated Dermatitis with Either High or Normal IgE Responded Well to Antiviral Therapy: A Study of 787 Quick-Tzanck-Test-Positive Patients Peer review status: No Corresponding Author: Dr. Lily Hsiao, Vice president, Moriya Eye and Skin Clinic, 5-7-1, Mizukino, 302-0121 - Japan Submitting Author: Dr. Lily Hsiao, Vice president, Moriya Eye and Skin Clinic, 5-7-1, Mizukino, 302-0121 - Japan Article ID: WMC004846 Article Type: Original Articles Submitted on:20-Mar-2015, 07:33:49 AM GMT Published on: 20-Mar-2015, 07:34:27 AM GMT Article URL: http://www.webmedcentral.com/article_view/4846 Subject Categories:DERMATOLOGY Keywords:herpes simplex virus, quick Tzanck test, erythema multiforme, atopic dermatitis, intrinsic atopic dermatitis How to cite the article:Hsiao L. Herpes Simplex Virus-Associated Dermatitis with Either High or Normal IgE Responded Well to Antiviral Therapy: A Study of 787 Quick-Tzanck-Test-Positive Patients. WebmedCentral DERMATOLOGY 2015;6(3):WMC004846 Copyright: This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source(s) of Funding: None Competing Interests: None Additional Files: HSVADWMC15 WebmedCentral > Original Articles Page 1 of 33 WMC004846 Downloaded from http://www.webmedcentral.com on 20-Mar-2015, 07:38:29 AM Herpes Simplex Virus-Associated Dermatitis with Either High or Normal IgE Responded Well to Antiviral Therapy: A Study of 787 Quick-Tzanck-Test-Positive Patients Author(s): Hsiao L Abstract Abbreviations Background: The overall age-adjusted 1. -
Sexually Transmitted Infections and Increased Risk of Co-Infection with Human Immunodeficiency Virus
REVIEW ARTICLE Sexually Transmitted Infections and Increased Risk of Co-infection with Human Immunodeficiency Virus Margaret R.H. Nusbaum, DO, MPH; Robin R. Wallace, MD; Lisa M. Slatt, MEd; Elin C. Kondrad, MD The incidence of trichomoniasis (Trichomonas vaginalis) Clinical Presentation in the United States is estimated at 5 million cases annu- Urethritis, Epididymitis, and Proctitis ally; chlamydia (Chlamydia trachomatis) at 3 million; gon- In men, STIs usually remain confined to the urethra. Symptoms orrhea (Neisseria gonorrhoeae), 650,000; and syphilis (Tre- of urethritis include urethral discharge, dysuria, or urethral ponema pallidum), 70,000. However, most sexually itching. The discharge of nongonococcal urethritis (NGU) is transmitted infections (STIs) are asymptomatic—con- often slight, and may not be apparent without massaging the tributing to underdiagnosis estimated at 50% or more. urethra. Discharge of NGU is usually minimal and gray, white, Diagnosis of an STI signals sexual health risk because an or mucoid rather than yellow. Discharge that is yellow and pre- STI facilitates the transmission and acquisition of other sent in greater volume most often signals infection with N STIs, including human immunodeficiency virus (HIV). gonorrhoeae. In fact, comorbid STIs increase patients’ susceptibility of Epididymitis presents as acute unilateral testicular pain acquiring and transmitting HIV by two- to fivefold. Sev- and swelling. Clinical findings include tenderness of the epi- eral studies have shown that aggressive STI prevention, didymis and ductus deferens, erythema and edema of the testing, and treatment reduces the transmission of HIV. overlying scrotal skin, urethral discharge, and dysuria. Swelling The authors discuss common clinical presentations, and tenderness may be localized or may extend to the entire screening, diagnosis, and treatment for trichomoniasis, epididymis and surrounding areas, making the epididymis less chlamydia, gonorrhea, syphilis, and herpes simplex virus. -
Uva-DARE (Digital Academic Repository)
UvA-DARE (Digital Academic Repository) Diagnostic aspects of human alphaherpesvirus infections in dermato-venerology Folkers, E. Publication date 1999 Link to publication Citation for published version (APA): Folkers, E. (1999). Diagnostic aspects of human alphaherpesvirus infections in dermato- venerology. General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl) Download date:07 Oct 2021 u-f 2 Tzanck smear and viral culture in diagnosis of herpes simplex virus and varicella-zoster virus infection Summary Herpes simplex virus and Varicella-zoster virus infections usually present a characteristic clinical picture. Tzanck smear and viral culture are just two of several laboratory tests to confirm the clinical diagnosis. The Tzanck smear especially is usefull for office diagnosis in dermatovenereological practice. The results of our investigations on this subject are described is this chapter. -
Pathology of Viral Disease Ila R. Singh, MD, Ph.D. March 21, 2003 1
Pathology of viral disease March 21, 2003 Ila R. Singh, M.D., Ph.D. Pathology of viral disease Topics for the first lecture…. General virology Ila Singh, MD, PhD Department of Pathology Viral lifecycle P & S 14-453 Viral pathogenesis [email protected] Laboratory diagnosis Viral Structure Virus size Herpes virus QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. Envelope Nucleocapsid Tegument Genome Spikes Principles of Virology: Molecular Biology, Pathogenesis, and Control, Principles of Virology: Molecular Biology, Pathogenesis, and Control S. J. Flint, L. W. Enquist, V. R. Racaniello, A. M. Skalka S. J. Flint, L. W. Enquist, V. R. Racaniello, A. M. Skalka Some useful terms Plaque pfu Viral life cycle MOI Particle to infectivity ratio Neutralizing Abs Cytopathic effect 1 Pathology of viral disease March 21, 2003 Ila R. Singh, M.D., Ph.D. E Entry into cells Viral assembly and release C Binding Internalization Budding Low-pH induced Protein conformational synthesis Assembly change Uncoating Replication of ER genome Fusion Nuclear transport Nuclear transport Methods of diagnosis for viral diseases I. Serology Look for viral antigens or anti-viral antibodies Serology Cytology or Histology A four fold or greater rise in titer between two serum specimens provides a positive diagnosis. Viral growth in cell culture Paired sera, the first taken as early as possible Detection of viral genome in the illness and the second 10 to 14 days after the onset of symptoms. Serology: ELISA Serology Methods ELISA )Rapid tests for Flu, RSV )Hep B, Hep C etc etc Western Blots 2 Pathology of viral disease March 21, 2003 Ila R. -
Lennette's Laboratory Diagnosis of Viral
Lennette’s Laboratory Diagnosis of Viral Infections NFECT I OUS I SEASE Fourth Edition Fourth I D Edition AN D THERAPY SER I ES About the book Volume 50 • Written from the perspective of the diagnostician, this bestselling book is the definitive text on the laboratory diagnosis of human viral diseases. • Contains a wealth of illustrations, tables, and algorithms to enhance your understanding of Viral Infections Viral of Lennette’s Lennette’s of this ever-evolving field. • A ready reference source for virologists, microbiologists, epidemiologists, laboratorians, infectious disease specialists and students. Laboratory Unique features • Has a new syndromic approach and discusses the differential diagnosis of potential causative agents along with suggestions for the appropriate diagnostic response. Diagnosis of • Examines the field’s rapid changes in technology, the continuing emergence of new viruses, and the newly described viral etiologies for clinical syndromes. Laboratory Diagnosis • Explores the increasingly important subject of molecular techniques in detail, covering the design of molecular tests, the importance of genotyping and viral Viral Infections sequence analysis, and the use of microarrays in diagnostic virology. Reviews of previous editions Fourth Edition a worthy addition to the bookshelves of the specialised virus laboratory as it contains a wealth of information not readily available elsewhere. The Journal of Clinical Pathology a comprehensive and valuable addition to any medical library. It will appeal to all students of virology, medical microbiology and infectious diseases. The information is set out in a user-friendly fashion and allows the reader to obtain pertinent information without being deluged with too many data [...] recommend[ed] highly. -
Laboratory Emerging Pathogens Initiative (Epi) Roll up Modifications Technical and User Manual
LABORATORY EMERGING PATHOGENS INITIATIVE (EPI) ROLL UP MODIFICATIONS TECHNICAL AND USER MANUAL PATCH LR*5.2*281 Version 5.2 June 2004 Department of Veterans Affairs VistA Health System Design and Development Preface The Veterans Health Information Systems and Architecture (VistA) Laboratory Emerging Pathogens Initiative (EPI) Rollup Modifications Patch LR*5.2*281 Technical and User Manual provides assistance for installing, implementing, and maintaining the EPI software application enhancements. Intended Audience The intended audience for this manual includes the following users and functionalities: • Veterans Health Administration (VHA) facility Information Resource Management (IRM) staff (will be important for installation and implementation of this package) • Laboratory Information Manager (LIM) (will be important for installation and implementation of this package) • Representative from the Microbiology section in support of the Emerging Pathogens Initiative (EPI) Rollup enhancements (i.e., director, supervisor, or technologist) (will be important for installation and implementation of this package especially with parameter and etiology determinations; may also have benefit from local functionality) • Total Quality Improvement/Quality Improvement/Quality Assurance (TQI/QI/QA) staff or persons at the VHA facility with similar function (will be important for implementation of this package given broad-ranging impact on medical centers and cross-cutting responsibilities that extend beyond traditional service lines; may also have benefit from local functionality) • Infection Control Practitioner (likely to have benefit from local functionality) NOTE: It is highly recommend that the Office of the Director (00) at each VHA facility designate a person or persons who will be responsible for the routine implementation of this patch (both at the time of this installation and afterwards) and to take the lead in trouble-shooting issues that arise with the routine functioning of the process. -
The Diagnostic Value of Tzanck Test in Vesiculobullous, Pustular, And
The Egyptian Journal of Hospital Medicine (April 2019) Vol. 75 (6), Page 3052-3059 The Diagnostic Value of Tzanck Test in Vesiculobullous, Pustular, and Oral Lesions in The Dermatology Clinic Amira Ahmed Adel Abdel Azim1, Gamal El-Din Abd El-Hamid El-Sayed2, Marwa Said Mahmoud1, Heba Ibrahim Mostafa Ibrahim1* 1Department of Dermatology and Venereology, 2Department of Pathology, Faculty of Medicine - Al-Azhar University *Corresponding author: Heba Ibrahim Mostafa Ibrahim, Mobile: (+20)01019899044, E-Mail: [email protected] ABSTRACT Background: Vesiculobullous disorders represent a heterogeneous group of dermatoses with protean manifestations. The accurate diagnosis of bullous disorders of the skin and mucous membrane requires evaluation of clinical, histological, and immunofluorescence findings. Objective: The aim of this study was to investigate Tzanck smear findings and to determine the diagnostic value of this test in moist (erosive, vesicular, bullous, and pustular) skin and oral lesions. Patients and Methods: This cross sectional study was conducted on one hundred patients presented with vesicular, bullous, and pustular skin lesions and/or oral lesions. All patients were included from Dermatology Outpatient Clinic at Al-Zahraa University Hospital over the period from March 2018 to March 2019. Results: Positivity of multinucleated giant cells in herpetic infection, acantholytic cells in pemphigus, dyskeratotic acantholytic cells and cocci in bullous impetigo were useful landmarks in Tzanck smear. A decrease in viral shedding from herpetic lesions of longer duration may correlate with the lower sensitivity of the Tzanck smear. Therefore, in this study we excluded the lesions older than 3 days. The percentage of positivity in vesicles was much higher than that of pustules. -
A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2013 Recommendations by the Infectio
Clinical Infectious Diseases Advance Access published July 10, 2013 IDSA GUIDELINES A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2013 Recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM)a Ellen Jo Baron,1,2 J. Michael Miller,3 Melvin P. Weinstein,4 Sandra S. Richter,5 Peter H. Gilligan,6 Richard B. Thomson Jr.,7 Paul Bourbeau,8 Karen C. Carroll,9 Sue C. Kehl,10 W. Michael Dunne,11 Barbara Robinson-Dunn,12 Joseph D. Schwartzman,13 14 15 16 17 17 17 Kimberle C. Chapin, James W. Snyder, Betty A. Forbes, Robin Patel, Jon E. Rosenblatt, and Bobbi S. Pritt Downloaded from 1Department of Pathology, Stanford University School of Medicine, Stanford, California; 2Cepheid, R&D, Sunnyvale, California; 3Microbiology Technical Services, LLC, Dunwoody, Georgia; 4Department of Medicine and Pathology, Robert Wood Johnson Medical School, New Brunswick, New Jersey; 5Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio; 6Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina; 7Department of Pathology, NorthShore University HealthSystem, Evanston, Illinois; 8Scientific Affairs, BD Diagnostics, Sparks, Maryland; 9Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland; 10Department of 11 Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin; bioMerieux, Inc., Durham, North Carolina, and Department of Pathology and http://cid.oxfordjournals.org/ -
A Practical Approach to Emergencies in the Neonatal Period
eona f N tal l o B a io n l r o u g y o J Peréz et al., J Neonatal Biol 2016, 5:1 Journal of Neonatal Biology DOI: 10.4172/2167-0897.1000213 ISSN: 2167-0897 Review Article Open access A Practical Approach to Emergencies in the Neonatal Period Alonso Mata-Peréz1, Manuel Soto-Martínez2 and Adriana Yock-Corrales1* 1Emergency Department, Hospital Nacional de Niños “Dr. Carlos Saenz Herrera”, San José, Costa Rica 2Respiratory Department, Hospital Nacional de Niños “Dr. Carlos Sáenz Herrera”, San José, Costa Rica *Corresponding author: Adriana Yock-Corrales, Servicio de Emergencias Pediátricas, Hospital Nacional de Niños “Dr. Carlos Sáenz Herrera”, Avenida Paseo Colón, San José, Costa Rica, Tel: (506) 8390-0516; E-mail address: [email protected] Received date: January 26, 2016; Accepted date: February 17, 2016; Published date: February 25, 2016 Copyright: © 2016 Peréz AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract All emergency departments should be prepared to care for a critically ill infant, including having the appropriate sized equipment. The most common diagnosis in admitted neonates include respiratory infections, sepsis, congenital heart disease, bowel obstruction, hypoglycemia and seizures. Febrile neonates are at high risk for sepsis and therefore need blood, urine and CSF testing. These patients should receive empiric antibiotic therapy in hospital. There are many life-threatening illness that can affect this population and is the responsibility of the emergency physician to assess the neonate, stabilize, narrow the differential diagnosis to the most likely and begin life-sustaining treatment. -
General Introduction •HHH
UvA-DARE (Digital Academic Repository) Diagnostic aspects of human alphaherpesvirus infections in dermato-venerology Folkers, E. Publication date 1999 Link to publication Citation for published version (APA): Folkers, E. (1999). Diagnostic aspects of human alphaherpesvirus infections in dermato- venerology. General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl) Download date:02 Oct 2021 $ 1 General introduction •HHH 1.1 Herpesviruses, important to mankind The herpesviruses (Herpesviridae) belong to a family of double-stranded DNA viruses. The origin and the evolution of herpesviruses is not known but presumably began about 200 Myears before present at the same time as the differentiation of species arose. An argument in support of this hypothesis is that more than 100 different herpesviruses have been characterized.