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Protocol to Establish Axenic Cultures for Diatoms of Fresh Water
International Journal of Science and Research (IJSR) ISSN (Online): 2319-7064 Index Copernicus Value (2013): 6.14 | Impact Factor (2015): 6.391 Protocol to Establish Axenic Cultures for Diatoms of Fresh Water Shristy Gautam1, Aditi Arya2, Vandana Vinayak3 1Diatom Research Lab, Department of Criminology & Forensic Science, School of Applied Sciences, Dr. H.S. Gour Central University, Sagar, M.P., 470003, India Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, 131039, India 2Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, 131039, India 3Diatom Research Lab, Department of Criminology & Forensic Science, School of Applied Sciences, Dr. H.S. Gour Central University, Sagar, M.P., 470003, India Abstract: Diatom axenic cultures have great potential importance in ecological, whole genome and other single genera based studies. The generation of axenic diatom cultures like other cell culture requires special treatment and requirement of macro, micronutrients vitamins and pH. However, since silica is the most important component of diatom cell wall it is most important component of the media too. The f/2 media with little modification served as the best media for fresh water diatoms collected from water bodies of Northern western India with culture conditions standardized at temperature 22°C, light intensity 18000 lumen per m2, 16 hours/8 hours of light:dark conditions. The present study demonstrates the protocol to hinder the growth of fungi, bacteria and other green algae which contaminates the diatom culture. It was observed that antibiotic mixture of penicillin G and streptomycin in the ratio of 1:0.5 removed most of the bacterial growth in the culture media. -
Community Composition and Status of Avifaunal Diversity in and Around Ottu Reservoir of Sirsa, Haryana, India
Published online: June 2, 2021 ISSN : 0974-9411 (Print), 2231-5209 (Online) journals.ansfoundation.org Research Article Community composition and status of avifaunal diversity in and around Ottu reservoir of Sirsa, Haryana, India Deepak Rai* Animal Behaviour and Wildlife Conservation Laboratory, Department of Zoology, Kurukshetra Article Info University, Kurukshetra- 136119 (Haryana), India https://doi.org/10.31018/ Vanita jans.v13i2.2666 Animal Behaviour and Wildlife Conservation Laboratory, Department of Zoology, Kurukshetra Received: April 1, 2021 University, Kurukshetra- 136119 (Haryana), India Revised: May 23, 2021 Accepted: May 27, 2021 *Corresponding author. Email: [email protected] How to Cite Rai, D. and Vanita (2021). Community composition and status of avifaunal diversity in and around Ottu reservoir of Sirsa, Haryana, India. Journal of Applied and Natural Science, 13(2), 593 - 606. https://doi.org/10.31018/jans.v13i2.2666 Abstract Assessments of avian communities’ composition in different habitats give an emphasis in monitoring environmental perspective. Depending on specific habitat functioning, their population pattern helps to determine the ecological health of an area. Bird sur- veys were accomplished on a fortnightly basis from October 2019 to September 2020 using Scan sampling, Point count and Line transect methods for evaluation of species abundance and richness in and around Ottu Reservoir, district Sirsa, Haryana (India). A total of 114 species comprising 91 genera, 47 families and 18 orders were recorded, among which 76 species were resident, 30 winter migrants and 8 summer migrants. Out of reported 114 species of birds, one species was vulnerable, 6 Near- threatened as listed by IUCN. Three bird species were listed under Schedule-I and the remaining species under Schedule-IV of IWPA (1972); six species under appendices of CITES (2012). -