MRPL10 Antibody

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Product Datasheet MRPL10 Antibody Catalog No: #AB34314 Package Size: #AB34314-1 50ul #AB34314-2 100ul Orders: [email protected] Support: [email protected] Description Product Name MRPL10 Antibody Host Species Rabbit Clonality Polyclonal Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Applications WB Species Reactivity Hu Specificity The antibody detects endogenous levels of total MRPL10 protein. Immunogen Type Peptide Immunogen Description Synthesized peptide derived from internal of human MRPL10. Target Name MRPL10 Other Names l10mt; mgc17973; mitochondrial ribosomal protein l10; mrp-l10; mrp-l8 Accession No. Swiss-Prot: Q7Z7H8NCBI Gene ID: 124995 SDS-PAGE MW 29kd Concentration 1.0mg/ml Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Store at -20°C Application Details Western blotting: 1:500~1:3000 Images Western blot analysis of extracts from HepG2 cells, COLO cells and 293 cells, using MRPL10 antibody #AB34314. Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial Address: 8400 Baltimore Ave. Suite 302 College Park MD 20740 USA http://www.abscitech.com 1 ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. The MGC Project Team; Genome Res. 14:2121-2127(2004). Kenmochi N., Genomics 77:65-70(2001). Koc E.C., J. Biol. Chem. 276:43958-43969(2001) Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave. Suite 302 College Park MD 20740 USA http://www.abscitech.com 2.

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Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation
From THE DEPARTMENT OF MICROBIOLOGY TUMOR AND CELL BIOLOGY (MTC) Karolinska Institutet, Stockholm, Sweden ROLE OF MITOCHONDRIAL RIBOSOMAL PROTEIN S18-2 IN CANCEROGENESIS AND IN REGULATION OF STEMNESS AND DIFFERENTIATION Muhammad Mushtaq Stockholm 2017 All previously published papers were reproduced with permission from the publisher. Published by Karolinska Institutet. Printed by E-Print AB 2017 © Muhammad Mushtaq, 2017 ISBN 978-91-7676-697-2 Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation THESIS FOR DOCTORAL DEGREE (Ph.D.) By Muhammad Mushtaq Principal Supervisor: Faculty Opponent: Associate Professor Elena Kashuba Professor Pramod Kumar Srivastava Karolinska Institutet University of Connecticut Department of Microbiology Tumor and Cell Center for Immunotherapy of Cancer and Biology (MTC) Infectious Diseases Co-supervisor(s): Examination Board: Professor Sonia Lain Professor Ola Söderberg Karolinska Institutet Uppsala University Department of Microbiology Tumor and Cell Department of Immunology, Genetics and Biology (MTC) Pathology (IGP) Professor George Klein Professor Boris Zhivotovsky Karolinska Institutet Karolinska Institutet Department of Microbiology Tumor and Cell Institute of Environmental Medicine (IMM) Biology (MTC) Professor Lars-Gunnar Larsson Karolinska Institutet Department of Microbiology Tumor and Cell Biology (MTC) Dedicated to my parents ABSTRACT Mitochondria carry their own ribosomes (mitoribosomes) for the translation of mRNA encoded by mitochondrial DNA. The architecture of mitoribosomes is mainly composed of mitochondrial ribosomal proteins (MRPs), which are encoded by nuclear genomic DNA. Emerging experimental evidences reveal that several MRPs are multifunctional and they exhibit important extra-mitochondrial functions, such as involvement in apoptosis, protein biosynthesis and signal transduction. Dysregulations of the MRPs are associated with severe pathological conditions, including cancer.
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1 AGING Supplementary Table 2
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Additional Tables.Xlsx
Additional Table 5 Enriched pathways of upregulated DEGs after spinal cord injury in WT (P < 0.05, q < 0.05) Database Description Ratio of DEGs Ratio of P -value P adjust q value gene name GO ribosomal subunit 87/755 214/12262 1.03E-49 4.61E-47 4.20E-47 Gm6576/Gm10036/Gm5786/Mrps28/Gm9493/Rpl13- ps3/Rpl7l1/Mrpl10/Mrpl44/Rps20/Mrpl51/Mrps11/Mpv17l2/Mrps7/Gm10020/Gm10073 /Mrpl2/Mrpl52/Rpl22/Mrpl4/Mrps24/Zcchc17/Rpl28/Mrpl34/Rpl29/Mrps36/Rpl9- ps6/Hba-a2/Hba-a1/Rpl23a- ps3/Rpl15/Rack1/Mrps18a/Mrpl28/Rpl10a/Mrpl12/Rpl18/Rpl35a/Mrpl41/Mrps12/Rpl36 a/Rps9/Rps18/Rps5/Rps27a/Rps24/Rplp2/Rpl14/Mrpl30/Rps6/Rpl6/Rps3/Rps4x/Rpsa/R pl7/Rpl27a/Rps23/Rps10/Mrps21/Rpl36/Rpl30/Rps17/Mrpl42/Rpl35/Rpl27/Rplp0/Rps28 /Rpl31/Rpl39/Rps13/Rpl13/Rps11/Rpl8/Rps3a1/Fau/Rpl9/Rpl3/Rpl26/Rps15/Rpl13a/Uba 52/Rpl24/Rpl19/Rps14/Rpl4/Rps27/Rplp1 GO ribosome 93/755 248/12262 1.67E-49 4.61E-47 4.20E-47 Gm6576/Pnpt1/Gm10036/Gm5786/Mrps28/Gm9493/Rpl13- ps3/Rpl7l1/Mrpl10/Mrpl44/Rps20/Mrpl51/Mrps11/Mpv17l2/Mrps7/Gm10020/Gm10073 /Mrpl2/Mrpl52/Rpl22/Mrpl4/Mrps24/Zcchc17/Rpl28/Mrpl34/Rpl29/Mrps36/Rpl9- ps6/Hba-a2/Hba-a1/Rpl23a- ps3/Rpl15/Rack1/Eif3h/Mrps18a/Mrpl28/Mrps33/Rpl10a/Mrpl12/Rpl18/Rpl35a/Btf3/Mrp l41/Mrps12/Rpl36a/Rps9/Rps18/Rps5/Rps27a/Rps24/Rplp2/Rpl14/Mrpl30/Rps6/Rpl6/R ps3/Rps4x/Rpsa/Rpl7/Rpl27a/Rps23/Rps10/Mrps21/Rpl36/Rpl30/Rps17/Mrpl42/Rpl35/R pl27/Rplp0/Rps28/Rpl31/Rpl39/Rps13/Rpl13/Rps11/Rpl8/Rps3a1/Fau/Rpl9/Rpl3/Rpl26/ Rps15/Rpl13a/Uba52/Rpl41/Rpl24/Rpl19/Rps14/Rpl4/Ndufa7/Rps27/Rplp1 GO structural constituent of 76/733 169/11958 1.65E-47 1.16E-44 1.12E-44 Gm6576/Gm10036/Gm5786/Rpl7l1/Mrpl10/Rps20/Mrpl51/Mrps11/Mrps7/Gm10020/G
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Cardiomyopathy Is Associated with Ribosomal Protein Gene Haploinsufficiency In
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MRPL10 Polyclonal Antibody
PRODUCT DATA SHEET Bioworld Technology,Inc. MRPL10 polyclonal antibody Catalog: BS65315 Host: Rabbit Reactivity: Human BackGround: term. Avoid freeze-thaw cycles. mitochondrial ribosomal protein L10(MRPL10) Homo Specificity: sapiens Mammalian mitochondrial ribosomal proteins are MRP-L10 Polyclonal Antibody detects endogenous levels encoded by nuclear genes and help in protein synthesis of MRP-L10 protein. within the mitochondrion. Mitochondrial ribosomes (mi- DATA: toribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different spe- cies, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical prop- Western Blot analysis of various cells using MRP-L10 Polyclonal Anti- erties, which prevents easy recognition by sequence ho- body diluted at 1：1000 mology. This gene encodes a 39S subunit protein. Se- quence analysis identified three transcript variants that encode two different isoforms. A pseudogene corre- sponding to this gene is found on chromosome 5q. [pro- vided by RefSeq, Nov 2010], Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Western blot analysis of 293T lysis using MRP-L10 antibody. Antibody Molecular Weight: was diluted at 1:1000 ~ 29 kDa Swiss-Prot: Q7Z7H8 Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific im- munogen. Western blot analysis of lysates from 293, HepG2, and COLO cells, us- Applications: ing MRPL10 Antibody. The lane on the right is blocked with the syn- Western Blot: 1/500 - 1/2000.
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bioRxiv preprint doi: https://doi.org/10.1101/580977; this version posted March 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Enhanced autophagic-lysosomal activity and increased BAG3- mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress Debapriya Chakraborty1, Vanessa Felzen1, Christof Hiebel1, Elisabeth Stürner1, Natarajan Perumal2, Caroline Manicam2, Elisabeth Sehn3, Franz Grus2, Uwe Wolfrum3, Christian Behl1* 1Institute of Pathobiochemistry, University Medical Center Mainz of the Johannes Gutenberg University, 55099 Mainz, Germany 2Experimental and Translational Ophthalmology, University Medical Center Mainz, 55131 Mainz, Germany 3 Institute for Molecular Physiology, Johannes Gutenberg University, 55128 Mainz, Germany E-mail addresses of authors: D. Chakraborty: [email protected]; V. Felzen: [email protected]; C. Hiebel: [email protected]; E. Stürner: [email protected]; N. Perumal: nperumal@eye- research.org; C. Manicam: [email protected]; E. Sehn: sehn@uni- mainz.de; F. Grus: [email protected]; U. Wolfrum: [email protected]; *Correspondence to Christian Behl, PhD Institute of Pathobiochemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6, D-55099 Mainz [email protected] Tel.: +49-6131-39-25890; Fax: +49-6131-39-25792 1 bioRxiv preprint doi: https://doi.org/10.1101/580977; this version posted March 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Oxidative stress and a disturbed cellular protein homeostasis (proteostasis) belong to the most important hallmarks of aging and of neurodegenerative disorders.
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Mitochondrial Ribosomal Proteins: Candidate Genes for Mitochondrial Disease James E
March/April 2004 ⅐ Vol. 6 ⅐ No. 2 review Mitochondrial ribosomal proteins: Candidate genes for mitochondrial disease James E. Sylvester, PhD1, Nathan Fischel-Ghodsian, MD2, Edward B. Mougey, PhD1, and Thomas W. O’Brien, PhD3 Most of the energy requirement for cell growth, differentiation, and development is met by the mitochondria in the form of ATP produced by the process of oxidative phosphorylation. Human mitochondrial DNA encodes a total of 13 proteins, all of which are essential for oxidative phosphorylation. The mRNAs for these proteins are translated on mitochondrial ribosomes. Recently, the genes for human mitochondrial ribosomal proteins (MRPs) have been identified. In this review, we summarize their refined chromosomal location. It is well known that mutations in the mitochondrial translation system, i.e., ribosomal RNA and transfer RNA cause various pathologies. In this review, we suggest possible associations between clinical conditions and MRPs based on coincidence of genetic map data and chromosomal location. These MRPs may be candidate genes for the clinical condition or may act as modifiers of existing known gene mutations (mt-tRNA, mt-rRNA, etc.). Genet Med 2004:6(2):73–80. Key Words: mitochondrial, ribosomal proteins, oxidative phosphorylation, candidate genes, translation Most of the energy requirements for cell growth, differenti- THE MITORIBOSOME ation, and development are met by the mitochondrial ATP Human cells contain two genomes and two protein synthe- produced by the process of oxidative phosphorylation. Mito- sizing (translation) systems. The first is the nuclear genome of chondrial DNA encodes 13 essential proteins of this oxidative 3 ϫ 109 bp that has 30,000 to 40,000 genes coding a much phosphorylation system.
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