DOCSLIB.ORG

Genetic Diversity and Population Structure of Tenacibaculum Maritimum, a Serious Bacterial Pathogen of Marine Fish: from Genome

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Genetic Diversity and Population Structure of Tenacibaculum Maritimum, a Serious Bacterial Pathogen of Marine Fish: from Genome Bridel et al. Vet Res (2020) 51:60 https://doi.org/10.1186/s13567-020-00782-0 RESEARCH ARTICLE Open Access Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fsh: from genome comparisons to high throughput MALDI-TOF typing Sébastien Bridel1,2,3, Frédéric Bourgeon4, Arnaud Marie2, Denis Saulnier5, Sophie Pasek6, Pierre Nicolas7, Jean‑François Bernardet1 and Eric Duchaud1* Abstract Tenacibaculum maritimum is responsible for tenacibaculosis, a devastating marine fsh disease. This flamentous bacterium displays a very broad host range and a worldwide geographical distribution. We analyzed and compared the genomes of 25 T. maritimum strains, including 22 newly draft‑sequenced genomes from isolates selected based on available MLST data, geographical origin and host fsh. The genome size (~3.356 Mb in average) of all strains is very similar. The core genome is composed of 2116 protein‑coding genes accounting for ~75% of the genes in each 1 genome. These conserved regions harbor a moderate level of nucleotide diversity (~0.0071 bp− ) whose analysis reveals an important contribution of recombination (r/m 7) in the evolutionary process of this cohesive species that appears subdivided into several subgroups. Association trends≥ between these subgroups and specifc geographi‑ cal origin or ecological niche remains to be clarifed. We also evaluated the potential of MALDI‑TOF‑MS to assess the variability between T. maritimum isolates. Using genome sequence data, several detected mass peaks were assigned to ribosomal proteins. Additionally, variations corresponding to single or multiple amino acid changes in several ribo‑ somal proteins explaining the detected mass shifts were identifed. By combining nine polymorphic biomarker ions, we identifed combinations referred to as MALDI‑Types (MTs). By investigating 131 bacterial isolates retrieved from a variety of isolation sources, we identifed twenty MALDI‑Types as well as four MALDI‑Groups (MGs). We propose this MALDI‑TOF‑MS Multi Peak Shift Typing scheme as a cheap, fast and an accurate method for screening T. maritimum isolates for large‑scale epidemiological surveys. Introduction as a response to environmental changes has been docu- Te rapid development of intensive aquaculture has mented for some important viruses and bacteria [3, 4]. been associated with a dramatic increase in outbreaks of In this context, the success and sustainability of aqua- infectious diseases [1, 2]. Additionally, the international culture largely depend on the understanding of the evo- spread of pathogens through the trade of fsh and eggs or lution and epidemiology of pathogens [1]. Among those, several species of the genus Tenacibaculum (family Fla- vobacteriaceae, phylum Bacteroidetes) are responsible for *Correspondence: [email protected] 1 Université Paris‑Saclay, INRAE, UVSQ, VIM 78350 Jouy‑En‑Josas, France diseases collectively designated as tenacibaculosis, a very Full list of author information is available at the end of the article serious bacterial condition of many commercial marine © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/publi cdoma in/ zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Bridel et al. Vet Res (2020) 51:60 Page 2 of 17 fsh species leading to considerable economic losses [5, species to draw a global picture of the genomic diversity 6]. Tenacibaculum maritimum (formerly Flexibacter of the species. In addition, we developed a genome-based maritimus) was the frst species to be characterized and MALDI-TOF MS scheme and typed 131 feld isolates. probably the best-known pathogen in the genus. More- While this technique is commonly used for species iden- over, T. maritimum can afect many feral, captive, and tifcation, bacterial characterization below the species cultured fsh species [5, 7] and has been repeatedly iden- level is much more challenging, requiring the identifca- tifed in many marine aquaculture systems worldwide. tion of subtle diferences between strains [22]. We also Diseased fsh usually exhibit a diversity of external symp- propose a dedicated website that allows both identifca- toms including corroded mouth, skin ulcers, fn necrosis, tion and typing of new Tenacibaculum isolates [23]. and rotted tail. Skin lesions are often colonized by oppor- tunistic pathogens such as Vibrio spp. So far, only one Materials and methods vaccine is commercially available, but it is restricted to Bacterial strains the protection of turbot. Hence, the control of T. mariti- Tenacibaclum maritimum strains were grown in marine mum outbreaks essentially relies on the use of antibiotics, 2216E broth (Difco, Becton, Dickinson and Co., Franklin sometimes combined with external disinfectants [8]. Lakes, New Jersey, USA) for 24 h at 28 °C and 170 rpm. Reliable methods for studying the relationships Stock cultures were preserved in marine 2216E broth between isolates of the same bacterial species (i.e., strain containing 20% (v/v) glycerol at −80 °C. Te 25 strains typing) are a key step for understanding the population used in this study are listed in Table 1 and the bacterial structure, the spreading and the epidemiology of patho- isolates subjected to MALDI-TOF MS analysis are listed gens. Diferent typing methods have been proposed for in Additional fle 1. epidemiological investigations of T. maritimum [9]. Tree serotypes displaying varying degrees of association with Genome sequencing, assembly and annotation host fsh species have been reported [10, 11] and difer- Following centrifugation of the liquid culture, genomic ent molecular technics have been used to determine the DNA was extracted from the pellet using the Wizard intraspecifc diversity of T. maritimum [12, 13]. Serologi- genomic DNA purifcation kit (Promega, Madison, Wis- cal data were compared with several PCR-based meth- consin, USA). Te genomes were sequenced using Illu- ods [14]. In 2014, we proposed a Multi Locus Sequence mina (HiSeq, 100 paired-end or MiSeq, 300 paired-end) Analysis (MLSA) scheme [15] that proved to be a pow- and genome assemblies were performed using Spades erful discriminating tool for isolate identifcation and and Velvet on the PATRIC website with default settings taxonomic afliation [16, 17]. MLSA revealed an unfore- [24]. Te resulting contigs (> 2000 bp) were integrated seen diversity including several, yet undescribed, patho- into the MicroScope platform [25]. genic species in Norway [18]. In addition, this 11-locus sequenced-based method revealed a high number of dis- Genome analysis and comparisons tinct genotypes for the species T. maritimum, suggest- Average Nucleotide Identity analyses were performed ing an endemic distribution of strains without signifcant using the ANIm method [26] with the Python module contribution of long-distance dissemination linked to Pyani [27] using proposed threshold of ≈ 95–96% for international fsh movements. More recently, whole-cell species delineation. Genome annotation, including man- matrix-assisted laser desorption ionization–time of fight ual curation, and comparisons, including pan and core mass spectrometry (MALDI-TOF MS) was used for the genome computation, were performed using the web diferentiation of several fsh-pathogenic Tenacibaculum interface MicroScope [28], which allows graphic visu- species [14, 19]. However, these studies did not reveal alization enhanced by a synchronized representation of any relationships between the proteomic profles and the synteny groups [29]. Persistent, shell and cloud genomes source of isolation of the strains for any of the Tenacib- were computed using the PPanGGOLiN 0.1.4 software aculum species analyzed, and no biomarker below the [30]. Considering the low levels of sequence divergence at species level (e.g., serotype-specifc peaks) was detected typical core genome loci previously reported for T. marit- for T. maritimum. Meanwhile, the complete genome imum [15], we chose a cutof of 80% identity and 80% on of the T. maritimum type strain [20] as well as the draft the minimal coverage of the length between the aligned genomes of the type strains and several feld isolates of portions of two proteins to determine whether two CDSs T. dicentrarchi and “T. fnnmarkense” have been recently were members of the same gene family. published [21], paving the way to comparative genomics. Te 25 genomes were aligned using Snippy [31] with In this study, we analyzed and compared 25 genomes the genome of strain NCIMB 2154T (the type strain of (including 22 newly draft-sequenced) of T. maritimum T. maritimum) [20] serving as a reference.
Load more

Recommended publications
  • Host-Parasite Interaction of Atlantic Salmon (Salmo Salar) and the Ectoparasite Neoparamoeba Perurans in Amoebic Gill Disease
    ORIGINAL RESEARCH published: 31 May 2021 doi: 10.3389/fimmu.2021.672700 Host-Parasite Interaction of Atlantic salmon (Salmo salar) and the Ectoparasite Neoparamoeba perurans in Amoebic Gill Disease † Natasha A. Botwright 1*, Amin R. Mohamed 1 , Joel Slinger 2, Paula C. Lima 1 and James W. Wynne 3 1 Livestock and Aquaculture, CSIRO Agriculture and Food, St Lucia, QLD, Australia, 2 Livestock and Aquaculture, CSIRO Agriculture and Food, Woorim, QLD, Australia, 3 Livestock and Aquaculture, CSIRO Agriculture and Food, Hobart, TAS, Australia Marine farmed Atlantic salmon (Salmo salar) are susceptible to recurrent amoebic gill disease Edited by: (AGD) caused by the ectoparasite Neoparamoeba perurans over the growout production Samuel A. M. Martin, University of Aberdeen, cycle. The parasite elicits a highly localized response within the gill epithelium resulting in United Kingdom multifocal mucoid patches at the site of parasite attachment. This host-parasite response Reviewed by: drives a complex immune reaction, which remains poorly understood. To generate a model Diego Robledo, for host-parasite interaction during pathogenesis of AGD in Atlantic salmon the local (gill) and University of Edinburgh, United Kingdom systemic transcriptomic response in the host, and the parasite during AGD pathogenesis was Maria K. Dahle, explored. A dual RNA-seq approach together with differential gene expression and system- Norwegian Veterinary Institute (NVI), Norway wide statistical analyses of gene and transcription factor networks was employed. A multi- *Correspondence: tissue transcriptomic data set was generated from the gill (including both lesioned and non- Natasha A. Botwright lesioned tissue), head kidney and spleen tissues naïve and AGD-affected Atlantic salmon [email protected] sourced from an in vivo AGD challenge trial.
    [Show full text]
  • Tenacibaculum Maritimum, Causal Agent of Tenacibaculosis in Marine Fish
    # 70 JANUARY 2019 Tenacibaculum maritimum, causal agent of tenacibaculosis in marine fish ICES IDENTIFICATION LEAFLETS FOR DISEASES AND PARASITES IN FISH AND SHELLFISH ICES INTERNATIONAL COUNCIL FOR THE EXPLORATION OF THE SEA CIEM CONSEIL INTERNATIONAL POUR L’EXPLORATION DE LA MER ICES IDENTIFICATION LEAFLETS FOR DISEASES AND PARASITES OF FISH AND SHELLFISH NO. 70 JANUARY 2019 Tenacibaculum maritimum, causal agent of tenacibaculosis in marine fish Original by Y. Santos, F. Pazos and J. L. Barja (No. 55) Revised by Simon R. M. Jones and Lone Madsen International Council for the Exploration of the Sea Conseil International pour l’Exploration de la Mer H. C. Andersens Boulevard 44–46 DK-1553 Copenhagen V Denmark Telephone (+45) 33 38 67 00 Telefax (+45) 33 93 42 15 www.ices.dk [email protected] Recommended format for purposes of citation: ICES 2019. Tenacibaculum maritimum, causal agent of tenacibaculosis in marine fish. Orig- inal by Santos Y., F. Pazos and J. L. Barja (No. 55), Revised by Simon R. M. Jones and Lone Madsen. ICES Identification Leaflets for Diseases and Parasites of Fish and Shell- fish. No. 70. 5 pp. http://doi.org/10.17895/ices.pub.4681 Series Editor: Neil Ruane. Prepared under the auspices of the ICES Working Group on Pathology and Diseases of Marine Organisms. The material in this report may be reused for non-commercial purposes using the rec- ommended citation. ICES may only grant usage rights of information, data, images, graphs, etc. of which it has ownership. For other third-party material cited in this re- port, you must contact the original copyright holder for permission.
    [Show full text]
  • First Isolation of Virulent Tenacibaculum Maritimum Strains
    bioRxiv preprint doi: https://doi.org/10.1101/2021.03.15.435441; this version posted March 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 First isolation of virulent Tenacibaculum maritimum 2 strains from diseased orbicular batfish (Platax orbicularis) 3 farmed in Tahiti Island 4 Pierre Lopez 1¶, Denis Saulnier 1¶*, Shital Swarup-Gaucher 2, Rarahu David 2, Christophe Lau 2, 5 Revahere Taputuarai 2, Corinne Belliard 1, Caline Basset 1, Victor Labrune 1, Arnaud Marie 3, Jean 6 François Bernardet 4, Eric Duchaud 4 7 8 9 1 Ifremer, IRD, Institut Louis‐Malardé, Univ Polynésie française, EIO, Labex Corail, F‐98719 10 Taravao, Tahiti, Polynésie française, France 11 2 DRM, Direction des ressources marines, Fare Ute Immeuble Le caill, BP 20 – 98713 Papeete, Tahiti, 12 Polynésie française 13 3 Labofarm Finalab Veterinary Laboratory Group, 4 rue Théodore Botrel, 22600 Loudéac, France 14 4 Unité VIM, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, France 15 * Corresponding author 16 E-mail: [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.03.15.435441; this version posted March 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 17 Abstract 18 The orbicular batfish (Platax orbicularis), also called 'Paraha peue' in Tahitian, is the most important 19 marine fish species reared in French Polynesia.
    [Show full text]
  • Full Article in Pdf Format
    DISEASES OF AQUATIC ORGANISMS Vol. 58: 1–8, 2004 Published January 28 Dis Aquat Org Phenotyphic characterization and description of two major O-serotypes in Tenacibaculum maritimum strains from marine fishes Ruben Avendaño-Herrera, Beatriz Magariños, Sonia López-Romalde, Jesús L. Romalde, Alicia E. Toranzo* Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago, 15782 Santiago de Compostela, Spain ABSTRACT: Tenacibaculum maritimum is the etiological agent of marine flexibacteriosis disease, with the potential to cause severe mortalities in various cultured marine fishes. The development of effective preventive measures (i.e. vaccination) requires biochemical, serological and genetic knowl- edge of the pathogen. With this aim, the biochemical and antigenic characteristics of T. maritimum strains isolated from sole, turbot and gilthead sea bream were analysed. Rabbit antisera were pre- pared against sole and turbot strains to examine the antigenic relationships between the 29 isolates and 3 reference strains. The results of the slide agglutination test, dot-blot assay and immunoblotting of lipopolysaccharides (LPS) and membrane proteins were evaluated. All bacteria studied were biochemically identical to the T. maritimum reference strains. The slide agglutination assays using O-antigens revealed cross-reaction for all strains regardless of the host species and serum employed. However, when the dot-blot assays were performed, the existence of antigenic heterogeneity was demonstrated. This heterogeneity was supported by immunoblot analysis of the LPS, which clearly revealed 2 major serological groups that were distinguishable without the use of absorbed antiserum: Serotypes O1 and O2. These 2 serotypes seem to be host-specfic. In addition, 2 sole isolates and the Japanese reference strains displayed cross-reaction with both sera in all serological assays, and are considered to constitute a minor serotype, O1/O2.
    [Show full text]
  • Penaeus Monodon
    www.nature.com/scientificreports OPEN Bacterial analysis in the early developmental stages of the black tiger shrimp (Penaeus monodon) Pacharaporn Angthong1,3, Tanaporn Uengwetwanit1,3, Sopacha Arayamethakorn1, Panomkorn Chaitongsakul2, Nitsara Karoonuthaisiri1 & Wanilada Rungrassamee1* Microbial colonization is an essential process in the early life of animal hosts—a crucial phase that could help infuence and determine their health status at the later stages. The establishment of bacterial community in a host has been comprehensively studied in many animal models; however, knowledge on bacterial community associated with the early life stages of Penaeus monodon (the black tiger shrimp) is still limited. Here, we examined the bacterial community structures in four life stages (nauplius, zoea, mysis and postlarva) of two black tiger shrimp families using 16S rRNA amplicon sequencing by a next-generation sequencing. Although the bacterial profles exhibited diferent patterns in each developmental stage, Bacteroidetes, Proteobacteria, Actinobacteria and Planctomycetes were identifed as common bacterial phyla associated with shrimp. Interestingly, the bacterial diversity became relatively stable once shrimp developed to postlarvae (5-day-old and 15-day- old postlarval stages), suggesting an establishment of the bacterial community in matured shrimp. To our knowledge, this is the frst report on bacteria establishment and assembly in early developmental stages of P. monodon. Our fndings showed that the bacterial compositions could be shaped by diferent host developmental stages where the interplay of various host-associated factors, such as physiology, immune status and required diets, could have a strong infuence. Te shrimp aquaculture industry is one of the key sectors to supply food source to the world’s growing pop- ulation.
    [Show full text]
  • Tenacibaculum Adriaticum Sp. Nov., from a Bryozoan in the Adriatic Sea
    International Journal of Systematic and Evolutionary Microbiology (2008), 58, 542–547 DOI 10.1099/ijs.0.65383-0 Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea Herwig Heindl, Jutta Wiese and Johannes F. Imhoff Correspondence Kieler Wirkstoff-Zentrum (KiWiZ) at the Leibniz-Institute for Marine Sciences, Am Kiel-Kanal 44, Johannes F. Imhoff D-24106 Kiel, Germany [email protected] A rod-shaped, translucent yellow-pigmented, Gram-negative bacterium, strain B390T, was isolated from the bryozoan Schizobrachiella sanguinea collected in the Adriatic Sea, near Rovinj, Croatia. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum, with sequence similarity levels of 94.8–97.3 % to type strains of species with validly published names. It grew at 5–34 6C, with optimal growth at 18–26 6C, and only in the presence of NaCl or sea salts. In contrast to other type strains of the genus, strain B390T was able to hydrolyse aesculin. The predominant menaquinone was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1. The DNA G+C content was 31.6 mol%. DNA–DNA hybridization and comparative physiological tests were performed with type strains Tenacibaculum aestuarii JCM 13491T and Tenacibaculum lutimaris DSM 16505T, since they exhibit 16S rRNA gene sequence similarities above 97 %. These data, as well as phylogenetic analyses, suggest that strain B390T (5DSM 18961T 5JCM 14633T) should be classified as the type strain of a novel species within the genus Tenacibaculum, for which the name Tenacibaculum adriaticum sp. nov. is proposed.
    [Show full text]
  • Tenacibaculosis Infection in Marine Fish Caused by Tenacibaculum Maritimum: a Review
    DISEASES OF AQUATIC ORGANISMS Vol. 71: 255–266, 2006 Published August 30 Dis Aquat Org REVIEW Tenacibaculosis infection in marine fish caused by Tenacibaculum maritimum: a review Ruben Avendaño-Herrera, Alicia E. Toranzo, Beatriz Magariños* Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain ABSTRACT: Tenacibaculum maritimum is the aetiological agent of an ulcerative disease known as tenacibaculosis, which affects a large number of marine fish species in the world and is of consider- able economic significance to aquaculture producers. Problems associated with epizootics include high mortality rates, increased susceptibility to other pathogens, high labour costs of treatment and enormous expenditures on chemotherapy. In the present article we review current knowledge on this bacterial pathogen, focusing on important aspects such as the phenotypic, serologic and genetic characterization of the bacterium, its geographical distribution and the host species affected. The epi- zootiology of the disease, the routes of transmission and the putative reservoirs of T. maritimum are also discussed. We include a summary of molecular diagnostic procedures, the current status of pre- vention and control strategies, the main virulence mechanisms of the pathogen, and we attempt to highlight fruitful areas for continued research. KEY WORDS: Tenacibaculum maritimum · Tenacibaculosis · Review · Pathogenicity · Diagnosis · Control Resale or republication not permitted without written consent of the publisher INTRODUCTION disease, gliding bacterial disease of sea fish, bacterial stomatitis, eroded mouth syndrome and black patch Tenacibaculum maritimum, a Gram-negative and necrosis have all been used. To avoid confusion with filamentous bacterium, has been described as the eti- other fish diseases, this review will use the name ological agent of tenacibaculosis in marine fish.
    [Show full text]
  • Advancements in Characterizing Tenacibaculum Infections in Canada
    pathogens Review Advancements in Characterizing Tenacibaculum Infections in Canada Joseph P. Nowlan 1,2,* , John S. Lumsden 1 and Spencer Russell 2 1 Department of Pathobiology, University of Guelph, Guelph, OT N1G 2W1, Canada; [email protected] 2 Center for Innovation in Fish Health, Vancouver Island University, Nanaimo, BC V9R 5S5, Canada; [email protected] * Correspondence: [email protected] Received: 10 November 2020; Accepted: 3 December 2020; Published: 8 December 2020 Abstract: Tenacibaculum is a genus of gram negative, marine, filamentous bacteria, associated with the presence of disease (tenacibaculosis) at aquaculture sites worldwide; however, infections induced by this genus are poorly characterized. Documents regarding the genus Tenacibaculum and close relatives were compiled for a literature review, concentrating on ecology, identification, and impacts of potentially pathogenic species, with a focus on Atlantic salmon in Canada. Tenacibaculum species likely have a cosmopolitan distribution, but local distributions around aquaculture sites are unknown. Eight species of Tenacibaculum are currently believed to be related to numerous mortality events of fishes and few mortality events in bivalves. The clinical signs in fishes often include epidermal ulcers, atypical behaviors, and mortality. Clinical signs in bivalves often include gross ulcers and discoloration of tissues. The observed disease may differ based on the host, isolate, transmission route, and local environmental conditions. Species-specific identification techniques are limited; high sequence similarities using conventional genes (16S rDNA) indicate that new genes should be investigated. Annotating full genomes, next-generation sequencing, multilocus sequence analysis/typing (MLSA/MLST), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), and fatty acid methylesters (FAME) profiles could be further explored for identification purposes.
    [Show full text]
  • Tenacibaculum Sp. Associated with Winter Ulcers in Sea-Reared Atlantic Salmon Salmo Salar
    Vol. 94: 189–199, 2011 DISEASES OF AQUATIC ORGANISMS Published May 9 doi: 10.3354/dao02324 Dis Aquat Org Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar A. B. Olsen1,*, H. Nilsen1, N. Sandlund2, H. Mikkelsen3, H. Sørum4, D. J. Colquhoun5 1National Veterinary Institute Bergen, 5811 Bergen, Norway 2Institute of Marine Research, 5817 Bergen, Norway 3Nofima Marin, 9291 Tromsø, Norway 4Norwegian College of Veterinary Medicine, 0033 Oslo, Norway 5National Veterinary Institute Oslo, 0106 Oslo, Norway ABSTRACT: Coldwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of winter ulcer cases in our laboratory revealed frequent presence in ulcers of long, slen- der rods clearly different from M. viscosa, a closer study focusing on these bacteria was conducted. Field cases of winter ulcers during 2 sampling periods, 1996 and 2004–2005, were investigated and long, slender rods were observed by histopathological examination in 70 and 62.5% of the ulcers examined, respectively, whereas cultivation on marine agar resulted in the isolation of yellow- pigmented colonies with long rods from 3 and 13% of the ulcers only. The isolates could be separated into 2 groups, both identified as belonging to the genus Tenacibaculum based on phenotypic charac- terization and 16S rRNA sequencing. Bath challenge for 7 h confirmed the ability of Group 1 bac- terium to produce skin and cornea ulcers. In fish already suffering from M.
    [Show full text]
  • Genomic Analyses of Polysaccharide Utilization in Marine Flavobacteriia
    Genomic Analyses of Polysaccharide Utilization in Marine Flavobacteriia Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften - Dr. rer. nat. - dem Fachbereich 2 Biologie/Chemie der Universität Bremen vorgelegt von Lennart Kappelmann Bremen, April 2018 Die vorliegende Doktorarbeit wurde im Rahmen des Programms International Max Planck Research School of Marine Microbiology“ (MarMic) in der Zeit von August 2013 bis April 2018 am Max-Planck-Institut für Marine Mikrobiologie angerfertigt. This thesis was prepared under the framework of the International Max Planck Research School of Marine Microbiology (MarMic) at the Max Planck Institute for Marine Microbiology from August 2013 to April 2018. Gutachter: Prof. Dr. Rudolf Amann Gutachter: Prof. Dr. Jens Harder Prüfer: Dr. Jan-Hendrik Hehemann Prüfer: Prof. Dr. Rita Groß-Hardt Tag des Promotionskolloquiums: 16.05.2018 Inhaltsverzeichnis Summary ............................................................................................................................................. 1 Zusammenfassung .............................................................................................................................. 3 Abbreviations ..................................................................................................................................... 5 Chapter I: General introduction ...................................................................................................... 6 1.1 The marine carbon cycle ........................................................................................................
    [Show full text]
  • Biological and Serological Characterization of a Non-Gliding Strain of Title Tenacibaculum Maritimum Isolated from a Diseased Puffer Fish Takifugu Rubripes
    NAOSITE: Nagasaki University's Academic Output SITE Biological and Serological Characterization of a Non-gliding Strain of Title Tenacibaculum maritimum Isolated from a Diseased Puffer Fish Takifugu rubripes Author(s) Rahman, Tanvir; Suga, Koushirou; Kanai, Kinya; Sugihara, Yukitaka Citation Fish Pathology, 49(3), pp.121-129; 2014 Issue Date 2014-10-02 URL http://hdl.handle.net/10069/35107 Right © 2014 日本魚病学会 This document is downloaded at: 2017-12-22T07:00:43Z http://naosite.lb.nagasaki-u.ac.jp 魚病研究 Fish Pathology, 49 (3), 121–129, 2014. 9 © 2014 The Japanese Society of Fish Pathology Research article Biological and Serological Characterization of a Non-gliding Strain of Tenacibaculum maritimum Isolated from a Diseased Puffer Fish Takifugu rubripes Tanvir Rahman1, Koushirou Suga1, Kinya Kanai1* and Yukitaka Sugihara2 1Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, Nagasaki 852-8521, Japan 2Nagasaki Prefectural Institute of Fisheries, Nagasaki 851-2213, Japan (Received April 1, 2014) ABSTRACT—Tenacibaculum maritimum is a Gram-negative, gliding marine bacterium that causes tena- cibaculosis, an ulcerative disease of marine fish. The bacterium usually forms rhizoid colonies on agar media. We isolated T. maritimum that formed slightly yellowish round compact colonies together with the usual rhizoid colonies from a puffer fish Takifugu rubripes suffering from tenacibaculosis, and studied the biological and serological characteristics of a representative isolate of the compact colony phenotype, designated strain NUF1129. The strain was non-gliding and avirulent in Japanese flounder Paralichthys olivaceus in immersion challenge test and showed lower adhesion ability to glass wall in shaking broth culture and to the body surface of flounder.
    [Show full text]
  • Features and Functions of Bacteria Associated with Phytoplankton Blooms
    REVIEWS Master recyclers: features and functions of bacteria associated with phytoplankton blooms Alison Buchan1, Gary R. LeCleir1, Christopher A. Gulvik2 and José M. González3 Abstract | Marine phytoplankton blooms are annual spring events that sustain active and diverse bloom-associated bacterial populations. Blooms vary considerably in terms of eukaryotic species composition and environmental conditions, but a limited number of heterotrophic bacterial lineages — primarily members of the Flavobacteriia, Alphaproteo- bacteria and Gammaproteobacteria — dominate these communities. In this Review, we discuss the central role that these bacteria have in transforming phytoplankton-derived organic matter and thus in biogeochemical nutrient cycling. On the basis of selected field and laboratory-based studies of flavobacteria and roseobacters, distinct metabolic strategies are emerging for these archetypal phytoplankton-associated taxa, which provide insights into the underlying mechanisms that dictate their behaviours during blooms. Autotrophs Phytoplankton, such as diatoms and coccolithophores, in a patchy distribution of bacterial activity throughout 6 Organisms that convert are free-floating photosynthetic organisms that are the oceans . Copiotrophic bacteria, which swiftly capital- inorganic carbon, such as CO2, found in aquatic environments. These organisms capture ize on increased carbon and nutrient concentrations at into organic compounds. energy from sunlight and transform inorganic matter both the microscale and macroscale, complement their Biological pump into organic matter (which is known as biomass). In the oligotrophic counterparts, which prefer dilute nutrient The export of phytosynthetically ocean, this organic matter is the foundation of a com- concentrations. Together, the heterotrophic bacteria, derived carbon via the sinking plex marine food web, which relies heavily on microbial which use these two distinct trophic strategies balance of particles from the illuminated transformation: approximately one-half of the carbon marine productivity.
    [Show full text]