D-JRP4-3.3 (MAD-VIR microarray analysis of minimum 500 samples)

st JRP5 - MAD-VIR - ET1 - 1 Call

Responsible Partner: SSI

GENERAL INFORMATION

European Joint Programme Promoting One Health in Europe through joint actions on foodborne full title zoonoses, antimicrobial resistance and emerging microbiological hazards European Joint Programme One Health EJP acronym Funding This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No 773830. Grant Agreement Grant agreement n° 773830 Start Date 01/01/2018 Duration 60 Months

DOCUMENT MANAGEMENT

JIP/JRP Deliverable D-JRP4-3.3 (MAD-VIR microarray analysis of minimum 500 samples) Join Integrative/Research JRP5 - MAD-VIR - ET1 - 1st Call Project JIP/JRP Leader Anders FOMSGAARD (SSI) Other contributors Maiken W Rosentierne (SSI) Due month of the deliverable 1st of April 2019 Actual submission month 29th of March 2019 Type R R: Document, report DEC: Websites, patent filings, videos, etc. OTHER Dissemination level CO PU: Public CO: confidential, only for members of the consortium (including the Commission Services)

MAD-VIR microarray analysis of minimum 500 samples WP2. Sample collection and analysis

Each partner in the project has selected different samples of interest from their biobanks to be tested with the PanVirus microarray in the 1st, 2nd and 3rd round at SSI. A common standardized sample pre- treatment/inactivation protocol was followed when possible.

To this date 509 of 671 samples have been tested by the PanVirus microarray. (Table 1 and Table 2). The samples are from wild life (rabbit, wild boar, duck, pheasant, stork, tortoise, hare, wolf, pigeon, springbook, deer, crow, pteropus vampyrus, partridge, eagle, owl, hedgehog and mouse), farm animals (sturgeon, trout, salmon, cat, goat, cow, chicken, horse, turkey, pig, sheep), and ZOO animals (elephant, lemur), aedes, ticks, other insects and human samples (See results in Supplementary Tables 1-5).

Table 1: Number of samples analyzed by PanVirus microarray INSTITUTE SSI 1ST ROUND SSI 2ND ROUND SSI 3RD ROUND PIWET 1ST ROUND INIA 1ST ROUND TOTAL PIWET 14/14 25/25 21/21 60/60 INIA 5/5 20/20 3/3 28/28 IZSAM 10/10 20/20 30/30 OIK 16/16 20/20 36/36 ANSES 8/8 20/20 28/28 VRI 3/3 20/20 25/25 48/48 IZSLER 12/12 46/46 58/58 UOS 0 20/20 20/20 APHA 0 17/17 17/17 SSI 43/43 76/76 29/155 148/357 TOTAL 111/111 284/284 54/180 21/21 3/3 473/599

SSI has sent 12 virus positive samples (purified or non-purified inactivated samples) to INIA, PIWET and APHA for ring-testing in 2018 and another 12 samples in 2019 (Table 2). All the samples were Quality Control for Molecular diagnostics (QCMD) samples from different test panels.

Table 2: Ring-test of selected samples from different QCMD test panels SAMPLE NO. Virus content Ring-test no.1 Virus SSI PIWET INIA APHA type 1 Zika virus RNA + + + + 2 Chikungunya virus RNA + +1,2 + + 3 Dengue virus type 2, West Nile virus, Yellow fever virus RNA + + + + 4 Measels virus RNA + + + + 5 Varicella Zoster virus DNA + - + - 6 Cytomegalovirus DNA + +3 + - 7 Adenovirus type 1 DNA + + + - 8 Human herpes virus type 1 DNA + + + - 9 Parechovirus type 1 RNA + + + + 10 Mumps RNA + + + + 11 Epstein–Barr virus DNA + +1,3 + - 12 Human herpes virus type 2 DNA + + + - Ring-test no.2 13 Human coronavirus OC43 RNA + 14 Human parainfluenza virus 2 RNA + 15 Enterovirus RNA + 16 Human parvovirus B19 DNA + 17 Human Herpesvirus type 5 DNA + 18 Human Herpesvirus type 1 DNA + 19 Zika Virus (French Polynesian) RNA + 20 Human Metapneumovirus A RNA + 21 Human adenovirus type 4 DNA + 22 HHV6 type A, HHV6 type B RNA + 23 Human rhinovirus A90 RNA + 24 Enterovirus 68 RNA + TOTAL 12/ 24 12/24 12/24 1very low signal 2low/strong signal during second testing 3low signal during second testing

Supplementary Table 1: 1st round at SSI

PCR No Name Species Material Virus present Array result Ct before/after WTA Usutu virus, Africa 2 Usutu RT-PCR 1 ANSES 1 Turdus merula brain Usutu virus lineage 22/18 WNV, lineage 1a, WNV RT-PCR 2 ANSES 2 Caballus equi brain Western Negative no ct/no ct Mediteranean clade WNV RT-PCR 3 ANSES 3 Caballus equi brain or isolate WNV, lineage 2 Negative no ct/no ct 4 Ornithodoros Negative (PanVirus array v2) ANSES 4 maritimus soft isolate Meaban virus Meaban virus (PanVirus array No PCR assay at SSI 5 tick v3) Japanese encephalitis virus, Japanese encephalitis Aedes pseudoscutellaris reovirus, JEV RT-PCR 6 ANSES 5 Tottori isolate isolate virus, genotype 3 Aedes albopictus densovirus, 13/6 Aedes albopictus densovirus 2 Avian rotavirus A, Hum.Rotavirus 7 ANSES 6 Caballus equi brain Avian rotavirus Turkey rotavirus A, RT-PCR Pheasant rotavirus A no ct/no ct VEEV RT-PCR 8 ANSES 7 Cell culture isolate VEEV VEEV 16/4 Eyach virus, Exogenous mouse mammary tumor virus, 9 ANSES 8 Ixodes ricinus brain Eyach virus No PCR assay at SSI Murine leukemia virus, Murine type C retrovirus, Spleen focus forming virus blood (dilution Usutu RT-PCR 10 INIA 1 Partridge 1:10 in BA1 Usutu Virus Avian myelocytomatosis virus no ct/no ct medium) blood (dilution WNV RT-PCR 11 INIA 12 Partridge 1:10 in BA1 WNV Lineage 2 WNV lin 2 25/26 medium) blood (dilution WNV lin 1, WNV RT-PCR 12 INIA 3 Partridge 1:10 in BA1 WNV Lineage 1 Kunjin virus 27/27 medium) WNV lin 1, WNV RT-PCR Kunjin virus, 28/34 Murine leukemia virus, Sample was tested 13 Murine osteosarcoma virus, with (#2) or without brain, macerated Murine type C retrovirus, Spleen (1#) Dnase treatment INIA 59 Mouse WNV Lineage 1 in PBS (1:10) focus forming virus after NA purification in order to verify the presence of 14 WNV lin 1 endogeneous retrovirus WNV lin 2, WNV RT-PCR Murine leukemia virus, 23/24 15 Murine osteosarcoma virus, Sample was tested Murine type C retrovirus, Spleen with (#2) or without focus forming virus (1#) Dnase treatment after NA purification in brain, macerated INIA 63 Mouse WNV Lineage 2 order to verify the in PBS (1:10) presence of endogeneous 16 negative retrovirus. BUT the DNAse treatment also removed the WNV signal !

Alphamesonivirus 1, Cavally virus, supernatant of Ngewotan virus (JKT9982), Usutu RT-PCR 17 ISZAM 4 Culex pipiens insect Usutu virus Houston virus, no ct/no ct homogenate Nam Dinh virus, Hana virus, Dak Nong virus 18 Negative (PanVirus array v2) supernatant of WNV RT-PCR IZSAM 1 Sparrow-hawk brain WNV Lineage 2 WNV lin 2 19 27/14 homogenate (PanVirus array v3) Ngewotan virus (JKT9982), Nam Dinh virus, Houston virus, Hana virus, 20 Dak Nong virus, Alphamesonivirus 1, Cavally virus (PanVirus array v2) Ngewotan virus (JKT9982), Nam Dinh virus, Houston virus, supernatant of Hana virus, WNV RT-PCR 21 IZSAM 2 Culex pipiens insect WNV Lineage 2 Dak Nong virus, 26/no ct homogenate Alphamesonivirus 1, Cavally virus (PanVirus array v2) Ngewotan virus JKT9982, Negev virus, Nam Dinh virus, Houston virus, 22 Dianke virus, Cavally virus, Alphamesonivirus 1, WNV lin 2 (PanVirus array v3)

Supernatant of Negative (PanVirus array v2) WNV RT-PCR 23 IZSAM 3 Crow brain WNV Lineage 2 32/no ct homogenate WNV lin 2 (PanVirus array v3) Supernatant of Usutu RT-PCR 24 IZSAM 5 Blackbird brain Usutu virus Usutu virus 22/not analyzed homogenate Bluetongue virus, Ovine enzootic nasal tumour Bluetongue virus, virus, 25 IZSAM 6 Sheep Whole blood No PCR assay at SSI serotype 3 Jaagsiekte sheep retrovirus, Enzootic nasal tumour virus of goats Supernatant of 26 Bluetongue virus, Negative IZSAM 7 Sheep spleen No PCR assay at SSI serotype 4 27 homogenate Negative supernatant of Bluetongue virus, Culicoides Bluetongue virus, 28 IZSAM 8 insect Caribou feces associated No PCR assay at SSI imicola serotype 4 homogenate gemycircularvirus Pteropine Orthoreovirus, Melaka orthoreovirus, Pteropus Supernatant of Pteropine 29 IZSAM 9 Pulau reovirus, No PCR assay at SSI vampyrus tissue culture Orthoreovirus Kampar orthoreovirus, Cangyuan orthoreovirus RVF RT-PCR 30 IZSAM10 Springbook Serum Rift valley fever virus Rift valley fever virus 30/16 Hum parvovirus PCR Canine parvovirus, IZSLER no ct/no ct 31 CAT Intestinal content PARVOVIRUS Feline astrovirus 2, 10 Hum astrovirus RT-PCR Feline leukemia virus 26/19

Turkey rotavirus A (all segments), Turkey astrovirus, Turkey astrovirus 3, Turkey astrovirus 2, Hum astrovirus RT-PCR IZSLER Turkey astrovirus 1, No ct/no ct 32 TURKEY intestinal content ASTRO + 13 Pheasant rotavirus A (5 Hum rotavirus RT-PCR segments), No ct/no ct Avastovirus 1, Turkey avisivirus, Avian rotavirus (6 segments), Gyrovirus GyV7 Pseudocowpox virus, orf virus, IZSLER Skin proliferative PARAPOX+++ Parapox PCR 33 BOVINE Bovine papular stomatitis virus, 15 lesions PAPILLOMA +++ 16/20 Bovine papillomavirus 2, Bovine papillomavirus 13 No PCR assay at SSI IZSLER European brown hare syndrome 34 HARE Liver LAGOVIRUS (EBHSV) 16 (1#) virus Sample was tested with (#2) or without (1#) Dnase treatment after NA purification in IZSLER European brown hare syndrome 35 HARE Liver LAGOVIRUS (EBHSV) order to verify the 16 (2#) virus presence of endogeneous retrovirus IZSLER Hum adeno PCR 36 PIGEON Intestinal content ADENOVIRUS Pigon adenovirus 1 21 No ct/no ct Y73 sarcoma virus, Turkey parvovirus, Rous sarcoma virus, Infectious bursal disease virus, Fowl adenovirus D, Fowl adenovirus 9, Fowl adenovirus 3, BIRNAVIRUS Fowl adenovirus 2, IZSLER 37 CHICKEN pool of organs (INFECTIOUS BURSAL Fowl adenovirus 11, 29 (2#) DISEASE VIRUS) chicken parvovirus, Caribou feces associated gemycircularvirus, No IBDV PCR assay at avian Sarcoma virus, SSI hum ADV PCR Aviam myelocytomatosis virus, no ct/no ct Avian leukosis, Avian leukemia virus, Sample was tested Avian adeno associated virus with (#2) or without Y73 sarcoma virus, (1#) Dnase treatment Turkey parvovirus, after NA purification in Rous sarcoma virus, order to verify the Infectious bursal disease virus, presence of Fowl adenovirus D, endogeneous Fowl adenovirus 9, retrovirus Fowl adenovirus 3, BIRNAVIRUS Fowl adenovirus 2, IZSLER 38 CHICKEN pool of organs (INFECTIOUS BURSAL Fowl adenovirus 11, 29 (1#) DISEASE VIRUS) Chicken parvovirus, Caribou feces associated gemycircularvirus, Avian Sarcoma virus, Avian myelocytomatosis virus, Avian leukosis, Avian leukemia virus, Avian adeno associated virus

Sewage associated gemycicircularvirus, Porcine rotavirus C, Porcine hemagglutinating Hum rota RT-PCR encephalomyelitis virus, 18/12 Nebovirus, Hum calici RT-PCR ROTAVIRUS & Human coronavirus OC43, no ct/no ct 39 IZSLER 3 BOVINE Faeces CORONAVIRUS Equine coronavirus, hum sapo RT-PCR Camel coronavirus HKU23, no ct/no ct Bovine rotavirus, hum coronavirus OC43 Bovine kobuvirus, 25/16 Bovine hungarovirus, Bovine coronavirus, Bovine calicivirus (lena) Y73 sarcoma virus, UR2 sarcoma virus, Rous sarcoma virus, 40 Avian sarcoma virus, Avian myelocytomatosis virus, Avian leukosis virus, IZSLER HERPES (AVIAN Avian leukemia virus CHICKEN pool of organs No PCR assay at SSI 31 LARINGOTRACHEITIS) Y73 sarcoma virus, UR2 sarcoma virus, Rous sarcoma virus, 41 Avian sarcoma virus, Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus Avian Leukemia virus Avian leukosis virus Avian myelocytomatosis virus, Skin lesions Fowlpox virus 42 IZSLER 5 CHICKEN ORTHOPOXVIRUS No PCR assay at SSI (crusts) Pinguinpox virus Pigeonpox virus, Reticuloendotheliosis virus, Rous sarcoma virus Pigeon circovirus, Columbid circovirus, 43 IZSLER 8 PIGEON Intestinal content REOVIRUS-LIKE Human papillomavirus type 10, No PCR assay at SSI Avian carcinoma virus, Chicken rotavirus G WNV RT-PCR West Nile virus WNV lin 2, 31/32 44 OKI 15 Human urine (lineage 2) JC virus JC PCR 34/17 WNV RT-PCR West Nile virus no ct/no ct 45 OKI 18 Human urine JC virus (lineage 2) JC PCR 26/15 West Nile virus WNV RT-PCR 46 OKI 2 Human urine WNV lin 2 (lineage 2) 32/16 West Nile virus WNV RT-PCR 47 OKI 24 Human urine negative (lineage 2) no ct/no ct BK virus 48 (PanVirus array v2) WNV RT-PCR BK virus 49 West Nile virus 38/19 OKI 25 Human urine (PanVirus array v2) (lineage 2) BK PCR WNV lin2, 30/21 50 BK virus (PanVirus array v3) West Nile virus WNV RT-PCR 51 OKI 30 Human urine WNV lin2 (lineage 2) 26/14 West Nile virus WNV RT-PCR 52 OKI 35 Human urine WNV lin2 (lineage 2) 28/17 West Nile virus WNV RT-PCR 53 OKI 39 Human urine WNV lin2 (lineage 2) 25/11 54 OKI 53 Human Serum Unknown negative 55 OKI 54 Human urine Unknown negative

Human herpesvirus 3 (VZV), Different samples from JC polyomavirus, the same patient were Micro Torque teno virus HD1, pooled togethter on 56 OKI 58 Human Serum Unknown Micro Torque teno virus HD14 1, the same array. Micro Torque teno virus HD14, OKI 58 Torque teno virus 16, VZV PCR Torque teno virus No ct/no ct Human herpesvirus 3 (VZV), JC PCR JC polyomavirus, No ct/ no ct Micro Torque teno virus HD1, OKI 59 57 OKI 59 Human urine Unknown Micro Torque teno virus HD14 1, VZV PCR Micro Torque teno virus HD14, 34/13 Torque teno virus 16, JC PCR Torque teno virus 31/14 58 OKI 65 Human Serum Unknown Negative 59 OKI 66 Human urine Unknown Negative 60 OKI 81 Human Serum Unknown Negative 61 OKI 82 Human urine Unknown Negative Porcine Kobuvirus, EV RT-PCR 62 PIWET 1 Wild boar serum Unknown porcine enterovirus, 29/12 Porcine circovirus PIWET 63 Horse serum Unknown Negative 10 internal organ PIWET homogenate 64 Tortoise Unknown Negative 11 (respiratory tract) internal organ PIWET homogenate 65 Tortoise Unknown Negative 12 (respiratory tract) Duck Hepatitis B Virus, Heron Hepatitis B virus, PIWET Homogenized parrot hepatitis B virus, Hum HBV RT-PCR 66 Stork Unknown 13 internal organs Ross goose hepatitis b virus, no ct/no co Sheldgoose hepatitis B virus, snow goose hepatitis B virus PIWET Homogenized Human HBV RT-PCR 67 Stork Unknown Heron Hepatitis B virus 14 internal organs no ct/no ct HEV RT-PCR 68 PIWET 2 Wild boar liver HEV gt 3 Swine hepatitis E virus 29/33 HEV RT-PCR 69 PIWET 3 Wild boar liver HEV gt 3 Swine hepatitis E virus 31/22 HEV RT-PCR Swine hepatitis E virus 26/18 Wild boar astrovirus, Rota RT-PCR porcine astrovirus, 27/27 Porcine bocavirus, EV RT-PCR 70 PIWET 4 Pig feces HEV gt 3 porcine enterovirus, 25/18 porcine kobuvirus, Hum astro RT-PCR rotavirus, no ct/no ct Posavirus 1, Hum sapo RT-PCR pig sapovirus no ct/no ct Swine hepatitis E virus swine pasivirus 1, HEV RT-PCR posavirus 1, 28/19 porcine teschovirus, Rota RT-PCR Porcine stool associated circular 71 27/22 PIWET 5 Pig feces HEV gt 3 virus, EV RT-PCR porcine kobuvirus, 27/19 porcine enterovirus, Hum astro RT-PCR porcine astrovirus, Parechovirus no ct/no ct like virus, wild boar astrovirus 72 Negative (PanVirus array v2) HEV RT-PCT PIWET 6 Rabbit serum HEV ra gt 73 Negative (PanVirus array v3) No ct/no ct

Sewage associated gemycircularvirus 5, Porcine parvovirus 2, Porcine kobuvirus, Porcine bocavirus 3, Porcine bocavirus 4, Porcine bocavirus 5, RVA RT-PCR 74 PIWET 7 Pig feces G4P6 RVA Po Circo like virus 21, No ct/no ct Mongoose feces associated gemycircularvirus c, Mongoose feces associated gemycircularvirus b, Human genital associated circular DNA virus 1 Porcine Rotavirus A, RVA RT-PCR 75 PIWET 8 Pig tissue culture G4P6 RVA Human rotavirus A 26/26 Snow goose hepatitis B virus, Sheldgoose hepatitis B virus, Ross goose hepatitis B virus, Human HBV RT-PCR 76 PIWET 9 Stork serum Unknown Parrot hepatitis B virus, no ct/no ct Heron hepatitis B virus, Duck hepatitis B virus 77 VRI 1 Human feces Unknown negative 78 VRI 2 Human Stool Unknown negative 79 VRI 3 Human CSF Unknown negative Tick (Ixodus Homogenized 80 SSI 1 unknown negative ricinus) pool of ticks Tick (Ixodus Homogenized 81 SSI 2 unknown negative ricinus) pool of ticks Tick (Ixodus Homogenized 82 SSI 3 unknown negative ricinus) pool of ticks Tick (Ixodus Homogenized 83 SSI 4 unknown negative ricinus) pool of ticks Tick (Ixodus Homogenized Blacklegged tick phlebovirus 1, 84 SSI 5 unknown No PCR assay at SSI ricinus) pool of ticks Blacklegged tick phlebovirus 2 Tick (Ixodus Homogenized 85 SSI 6 unknown negative ricinus) pool of ticks Blacklegged tick phlebovirus 1, Tick (Ixodus Homogenized Uukuniemi RT-PCR 86 SSI 7 unknown Blacklegged tick phlebovirus 2, ricinus) pool of ticks na/22 Uukuniemi virus Tick (Ixodus Homogenized 87 SSI 8 unknown negative ricinus) pool of ticks Simian Agent 10, Different samples from 88 SSI 9 Human Lung secretion unknown JC polyomavirus, the same patient were Human parainfluenza virus 3 pooled togethter on Simian Agent 10, the same array. 89 SSI 10 Human Urine unknown JC polyomavirus, SSI 9 PIV-3 RT-PCR Human parainfluenza virus 3 12/7 Simian Agent 10, SSI 10 JC PCR 90 SSI 11 Human EDTA-blod unknown JC polyomavirus, 30/11 Human parainfluenza virus 3 Supernantant CCHF RT-PCR 91 SSI 12 SSI Ctrl CCHF ibar CCHFV from cell culture 27/19 Supernantant CCHFV, CCHF RT-PCR 92 SSI 13 SSI Ctrl CCHF agf09 from cell culture PIV-5 28/22 Supernantant EBOV RT-PCR 93 SSI 14 SSI Ctrl EBOV Zaire EBOV Zaire from cell culture 29/35 Supernantant EBOV RT-PCR 94 SSI 15 SSI Ctrl EBOV sudan EBOV Sudan from cell culture 29/33 Supernantant MARV RT-PCR 95 SSI 16 SSI Ctrl MARV popp MARV from cell culture 22/6 Supernantant MARV, MARV RT-PCR 96 SSI 17 SSI Ctrl MARV mukose from cell culture PIV-5 26/20 Supernantant LASV RT-PCR 97 SSI 18 SSI Ctrl LASV jos LASV from cell culture 30/26 Supernantant Negative LASV RT-PCR 98 SSI 19 SSI Ctrl LASV av2 from cell culture LASV 31/35

Serum spiked MERS RT-PCR 99 SSI 20 EQA-panal MERS MERS with MERS (#15) 18/6 Supernantant Sin Nombre RT-PCR 100 SSI 21 SSI Ctrl Sin nombre negative from cell culture no ct/no ct Supernantant Seoul RT-PCR 101 SSI 22 SSI Ctrl Seoul negative from cell culture no ct/no ct Supernantant Puumala virus RT-PCR 102 SSI 23 SSI Ctrl Puumala negative from cell culture no ct/no ct Supernantant DOBV RT-PCR 103 SSI 24 SSI Ctrl Dobrava DOBV from cell culture 29/20 Supernantant Hantaan RT-PCR 104 SSI 25 SSI Ctrl Hantaan Hantaan virus from cell culture 27/20

Tick (Ixodus Homogenized Negative (PanVirus array v2) TBE RT-PCR 105 SSI 26 TBE ricinus) pool of ticks TBE 29/34 106 SSI 27 Human Biopsy from brain ukendt negative HEV RT-PCR 107 SSI 28 Human EDTA-blod HEV Hepatitis E virus 28/20 Hepatitis E virus, HEV RT-PCR 108 SSI 29 Human EDTA-blod HEV Swine Hepatitis E virus 27/15 Plasma spiked HEV RT-PCR 109 SSI 30 QCMD-panel HEV gg3f Swine hepatitis E virus with HEV (14-01) 34/16 Plasma spiked Swine hepatitis E virus, HEV RT-PCR 110 SSI 31 QCMD-panel HEV gg3c with HEV (14-05) Torque teno virus 1 34/12 Sindbis virus, Plasma spiked Sindbis like virus, Sindbis RT-PCR 111 SSI 32 SSI Ctrl with Sindbis cell Sindbis Ockelbo virus 35/no ct culture Babanki virus 112 SSI 33 Human EDTA blood unknown negative

113 SSI 34 Human serum/plasma unknown negative 114 SSI 35 Human KF unknown negative 115 SSI 36 Human serum unknown negative 116 SSI 37 Human EDTA blood unknown negative

117 SSI 38 Human serum/plasma unknown negative

118 SSI 39 Human biopsy unknown negative Cowpox PCR 119 SSI 40 Human Unknown Cowpox Cowpox 24/30 NA from Rabies virus RT-PCR 120 SSI 41 fox Rabies virus Rabies virus greenland foxes 17/12 Different samples from the same patient were 121 SSI 42 Human serum unknown pooled togethter on the same array. TTV-16 SSI 42 : TTV PCR No ct/15 122 SSI 43 Human unknown spinal SSI43 : TTV PCR No ct/no ct

Supplementary Table 2: 1st round at PIWET

No Name Species Material Virus present Array result Remarks Po Circo like virus 21, Exp.4.Cy5 faecal Bovine kobuvirus, 123 cattle unknown Not confirmed by PCR 1.1 suspension Sewage associated gemycircularvirus 4 Porcine teschovirus, Porcine rotavirus C, Exp.4.Cy5 faecal Rotavirus group A, Additional virus not 124 pig Porcine rotavirus A, 1.2 suspension GXP[14] genotype confirmed by PCR Porcine kobuvirus, Porcine enterovirus,

Porcine astrovirus 4, Po Circo like virus 51, Astrovirus wild boar Enzootic nasal tumour virus of peripheral Exp.4.Cy3 goats, Maedi Visna virus not 125 goat blood Maedi Visna Virus 1.3 Jaagsiekte sheep retrovirus, confirmed by PCR leukocytes Ovine enzootic nasal tumour virus Posavirus 1, Porcine teschovirus, Porcine kobuvirus, Porcine enterovirus, Porcine bocavirus, Exp.4.Cy5 faecal Rotavirus group A, Additional virus not 126 pig Porcine astrovirus 4, 1.3 suspension GXP[14] genotype confirmed by PCR Astrovirus wild boar, Swine pasivirus 1, Porcine astrovirus 2, Porcine circovirus, Parechovirus like virus Exp.4.Cy5 127 wild boar serum unknown negative 1.4 Exp.4.Cy5 serum + brain 128 pig unknown negative 1.1 homogenate Rabbit rotavirus A, Exp.4.Cy3 faecal Rotavirus group A, Sewage associated Additional virus not 129 rabbit 1.2 suspension G3P[13] genotype gemycircularvirus 4, confirmed by PCR Rabbit calicivirus Exp.4.Cy5 130 cat tissue culture Feline calicivirus Feline calicivirus Not confirmed by PCR 1.2 Exp.4.Cy5 faecal 131 cattle unknown negative 1.3 suspension internal organ homogenate 132 1 Turtle unknown negative (respiratory tract) internal organ homogenate 133 1 Turtle unknown negative (respiratory tract) DNA extracted bovine foamy virus Prescence of BFV not 134 1 deer negative from blood (BFV) confirmed by PCR Fowl adenovirus 9, Fowl adenovirus 3, RNA isolated Fowl adenovirus 2, Additional virus not 135 1 Stork from internal Adenovirus type 11 Fowl adenovirus 11, confirmed by PCR organs Avian Leukemia virus, Avian leukosis virus very low signals, liver HEV ra (rabbit) 136 2 rabbit negative experiments has to be homogenate genotype repeated very low signals, DNA extracted BFV, BIV, BoHV-4, 137 2 cattle negative experiments has to be from blood BoHV-6 repeated very low signals, liver 138 2 wild boar HEV genotype 3 negative experiments has to be homogenate repeated very low signals, liver HEV ra (rabbit) 139 2 rabbit negative experiments has to be homogenate genotype repeated liver 140 3 wild boar HEV genotype 3 negative re-analysed sample homogenate DNA extracted BFV, BIV, BoHV-4, 141 3 cattle negative re-analysed sample from PBLs BoHV-6 very low signals, liver HEV ra (rabbit) 142 3 rabbit negative experiments has to be homogenate genotype repeated liver HEV ra (rabbit) 143 3 rabbit negative re-analysed sample homogenate genotype

Supplementary Table 3: 1st round at INIA

No Name Species Material Virus present Array result Remarks blood (dilution Red-legged WNV lin 1, 144 INIA 3 1:10 in BA1 WNV Lineage 1 partridge Kunjin virus medium) WNV lin 1, Kunjin virus, brain, Murine leukemia virus, Additional virus not 145 INIA 59 Mouse macerated in WNV Lineage 1 Murine osteosarcoma virus, confirmed by PCR PBS (1:10) Murine type C retrovirus, Spleen focus forming virus Usuv grey brain, Usutu virus, Additional virus not 146 partridge Grey partridge macerated in Usutu virus Reticuloendotheliosis virus confirmed by PCR heart PBS (1:10)

Supplementary Table 4: 2nd round at SSI

PCR confirmation Virus ct-values Confirmation No Name Species Material Array result content before/after by owner WTA No virus was 147 VRI 1.2 Human CSF Unknown Negative identified previously No virus was 148 VRI 2.2 Human CSF Unknown Negative identified previously virus sample HSV1 PCR 149 VRI 3.2 Cultured virus cultured in Blinded Human Herpesvirus type 1 Correct, HSV1 17/6 cell culture virus sample Zika RT-PCR Correct, Zika 150 VRI 4.2 Cultured virus cultured in Blinded Zika virus 12/9 virus (Africa) cell culture TBE virus, virus sample Spanish goat encephalitis virus, TBE RT-PCR Correct, TBE 151 VRI 5.2 Cultured virus cultured in Blinded Negishi virus, 15/17 (Neudoerfl) cell culture Louping ill virus Correct, Louping ill Louping ill virus, virus sample No RT-PCR assay virus Negishi virus, 152 VRI 6.2 Cultured virus cultured in Blinded at SSI (recorded Spanish goat encephalitis virus, cell culture wrongly as TBE Powassan virusat VRI) virus sample RVFV RT-PCR 153 VRI 7.2 Cultured virus cultured in Blinded Rift Valley fever virus Correct, RVFV 24/5 cell culture virus sample WNV RT-PCR Correct, WNV 154 VRI 8.2 Cultured virus cultured in Blinded WNV lin 1 16/3 lin 1 cell culture virus sample WNV RT-PCR Correct, WNV 155 VRI 9.2 Cultured virus cultured in Blinded WNV lin 2 No ct/29 lin 2 cell culture Correct, virus sample No PCR array at Porcine 156 VRI 10.2 Cultured virus cultured in Blinded Porcine cytomegalovirus SSI cytomegalovir cell culture us TBE, virus sample Spanish goat encephalitis virus, TBE RT-PCR Correct, TBE 157 VRI 11.2 Cultured virus cultured in Blinded Omsk hemorrhagic fever virus, 18/17 (hybr) cell culture Negishi virus, Louping ill virus virus sample Zika RT-PCR Correct, Zika 158 VRI 12.2 Cultured virus cultured in Blinded Zika virus 11/7 virus (Brazil) cell culture

Infectious pancreatic necrosis Correct, virus sample virus, No PCR array at Infectious 159 VRI 13.2 Cultured virus cultured in Blinded Paralichthys olivaceus birnavirus, SSI pancreatic cell culture Marine birnavirus necrosis virus virus sample No PCR array at 160 VRI 14.2 Cultured virus cultured in Blinded Pestivirus type 1 Correct, BVDV SSI cell culture virus sample Kyasanur forest disease virus, PanFlavi RT-PCR 161 VRI 15.2 Cultured virus cultured in Blinded Correct, KFDV Alkhumra hemorrhagic fever virus 16/8 cell culture West Nile virus lin 2, Spleen focus forming virus, Murine type C retrovirus, virus sample Murine leukemia virus, WNV RT-PCR Correct, WNV 162 VRI 16.2 Cultured virus cultured in Blinded Friend murine leukemia virus, 20/7 3 (99-222) cell culture Murine osteosarcoma virus, Rauscher murine leukemia virus, Abelson murine leukemia virus, Moloney murine leukemia virus Rabies virus, Spleen focus forming virus, Murine type C retrovirus, virus sample Murine leukemia virus, Rabies RT-PCR Correct, 163 VRI 17.2 Cultured virus cultured in Blinded Murine osteosarcoma virus, 17/23 Rabies cell culture Moloney murine leukemia virus, Exogenous mouse mammary tumor virus virus sample Correct, WNV 164 VRI 18.2 Cultured virus cultured in Blinded WNV lin 2 Not analyzed yet ! Lin 2 cell culture Langat virus, Spleen focus forming virus, Murine type C retrovirus, virus sample Murine osteosarcoma virus, No PCR array at Correct, 165 VRI 19.2 Cultured virus cultured in Blinded Murine leukemia virus, SSI Langat virus cell culture Mouse mammary tumor virus, Exogenous mouse mammary tumor virus, Abelson murine leukemia virus No virus was 166 VRI 20.2 Human CSF unknown Negative identified previously. GB virus C/Hepatitis G virus, Swine Correct, 167 OKI/1.2 human Serum Blinded hepatitis E virus, Not analyzed yet ! Hepatitis E Torque teno virus HD23 virus Correct, 168 OKI/2.2 human Serum Blinded Hepatitis A virus Not analyzed yet ! Hepatitis A virus Correct, Throat 169 OKI/3.2 human Blinded Influenza A virus H3N2 (2004) Not analyzed yet ! Influenza A swabs virus (H3N2) Correct, 170 OKI/4.2 human BAL Blinded Influenza A virus H1N1 (2009) Not analyzed yet ! Influenza A virus (H1N1) WNV RT-PCR Correct, WNV 171 OKI/5.2 human urine Blinded WNV lin2 32/18 Lin 2 Dengue virus type 1, DENV RT-PCR 172 OKI/6.2 human Serum Blinded Correct, DENV Torque teno virus type 16 28/14 Correct, CHIKV RT-PCR 173 OKI/7.2 human Whole blood Blinded Chikungunya virus Chikungunya 32/18 virus Rabies virus, Spleen focus forming virus brain Rabies RT-PCR Correct, 174 OKI/8.2 human Blinded Murine type C retrovirus, homogenate 17/7 Rabies virus Murine leukemia virus, Mouse parvovirus Measels virus, Measels RT-PCR Correct, 175 OKI/9.2 human Urine Blinded Human papillomavirus type 107 27/16 Measels virus

Correct, Enterovirus A71, EV RT-PCR 176 OKI/10.2 human stool Blinded Enterovirus Coxsackievirus A5 31/12 A71 Sample A: Different samples OKI/11.2 intestine from the same No virus was 177/ A homogenate patient were human Unknown Negative identified 178 OKI/11.2 Sample B: pooled togethter previously. B Lung on the same homgenate array. Human coxsackievirus A6, Enterovirus A71, Correct, Throat Coxakie A EV RT-PCR 179 OKI/12.2 human Coxsackievirus EVA, Coxakie A swabs virus , 23/5 Coxsackievirus A6, Coxsackievirus virus A12 HCV confirmed by WNV RT-PCR PCR and NGS. 32/28 180 OKI/13.2 human Whole blood WNV Hepatitis C genotype 1 WNV HCV RT-PCR confirmed by 24/31 PCR but not NGS. WNV lin2, Merkel cell polyomavirus, WNV RT-PCR Correct, WNV 181 OKI/14.2 human Urine WNV lin 2 Human papillomavirus type 90, 31/not analyzed lin 2 Human papillomavirus type 30, Human papillomavirus type 110 LCMV positive JC virus only in 182 OKI/15.2 human Urine LCMV (?) JC virus confirmed by serology, not NGS PCR WNV RT-PCR Correct, WNV 183 OKI/16.2 human Whole blood WNV lin 2 WNV lin 2 27/not analyzed lin 2 Sample A: Different samples OKI/17.2 Whole blood from the same No virus was 184/ A human (EDTA); Unknown Negative patient were identified 185 OKI/17.2 Sample B: pooled togethter previously B Urine on the same array No virus was 186 OKI/18.2 human urine Unknown Negative Not analyzed yet ! identified previously Influenza B Influenza B virus Lee 1940, Human Correct, Tracheal 187 OKI/19.2 human Yamagata coronavirus 229E, Not analyzed yet ! Influenza B swabs virus MW polyomavirus virus Influenza B Correct, Throat 188 OKI/20.2 human Victoria Influenza B virus Lee 1940 Not analyzed yet ! Influenza B swabs virus virus (Cyprinid herpesvirus 2) 189 Not Correct, 18/280.2 Piscine (analysed on PanVirus array v3) ANSES F1 heart No PCR at SSI! Piscine Salmon reovirus (Cyprinid herpesvirus 2) 190 reovirus 1 (analysed on PanVirus array v3.1) Negative 191 Not Correct, 18/280.3 Piscine (analysed on PanVirus array v3) ANSES F2 heart No PCR at SSI! Piscine salmon reovirus Negative 192 reovirus 1 (analysed on PanVirus array v3.1) Negative 193 Not Correct, 18/280.4 Piscine (analysed on PanVirus array v3) ANSES F3 heart No PCR at SSI! Piscine Salmon reovirus Negative 194 reovirus 1 (analysed on PanVirus array v3.1) Negative 195 Not Correct, 18/280.5 Piscine (analysed on PanVirus array v3) ANSES F4 heart No PCR at SSI! Piscine Salmon reovirus Negative 196 reovirus 1 (analysed on PanVirus array v3.1) Not Correct, Piscine Negative 197 ANSES F5 18/361 Salmon heart No PCR at SSI! Piscine reovirus reovirus 1 Not Correct, Piscine Negative 198 ANSES F6 18/362 Salmon heart No PCR at SSI! Piscine reovirus reovirus 1

Not Correct, Piscine Negative 199 ANSES F7 18/363 Salmon heart No PCR at SSI! Piscine reovirus reovirus 1 18/370 Cyprinid herpesvirus 2 Not Correct, 200 ANSES F8 gills AcIV-E No PCR at SSI! Sturgeon AcIV-E 18/371 Correct, AcIV- 201 ANSES F9 gills AcIV-E Acipenser Iridovirus European No PCR at SSI! Sturgeon E ANSES 18/372 Correct, AcIV- 202 gills AcIV-E Acipenser Iridovirus European No PCR at SSI! F10 Sturgeon E ANSES No, Piscine 203 X1 Blinded Blinded Negative No PCR at SSI! F11 reovirus1 Piscine reovirus isolate CGA280 Correct, ANSES (all 10 segments), Piscine 204 X2 Blinded Blinded No PCR at SSI! F12 Reovirus sp Salmo (all 10 reovirus1 segments) Reovirus sp Salmo, Correct, ANSES 205 X3 Blinded Blinded Piscine reovirus isolate CGA280, No PCR at SSI! Piscine F13 (Cyprinid herpesvirus 2) reovirus 3 No virus was ANSES 206 X4 Blinded Unknown Negative identified F14 previously ANSES Not correct, 207 X5 Blinded Blinded (Cyprinid herpesvirus 2) No PCR at SSI! F15 AcIV-E ANSES Not correct, 208 X6 Blinded Blinded (Cyprinid herpesvirus 2) No PCR at SSI! F16 AcIV-E ANSES Not correct, 209 X7 Blinded Blinded (Cyprinid herpesvirus 2) No PCR at SSI! F17 AcIV-E Cyprinid herpesvirus 2, Correct, ANSES 210 X8 Blinded Blinded Piscine reovirus, No PCR at SSI! Piscine F18 short finned eel ranavirus reovirus 1 Correct, ANSES Piscine reovirus, 211 X9 Blinded Blinded No PCR at SSI! Piscine F19 Reovirus sp Salmo reovirus 1 Correct, ANSES Piscine reovirus, 212 X10 Blinded Blinded No PCR at SSI! Piscine F20 Reovirus sp Salmo reovirus 1 Not correct, 213 APHA 1 Pig Brain Blinded Negative No PCR at SSI! Porcine Pestivirus Correct, 214 APHA 2 Elephant Heart Blinded Elephant herpesvirus 1 No PCR at SSI! EEHV-1A Correct, 215 APHA 3 Elephant Muscle Blinded Elephant herpesvirus 5 No PCR at SSI! EEHV-5 Not correct, 216 APHA 4 Pig Heart Blinded Negative No PCR at SSI! Porcine parvovirus Not correct, Turkey hepatitis virus, Turkey Turkey gallivirus, 217 APHA 5 Turkey Faeces Blinded Not analyzed yet ! Astrovirus Turkey rotavirus A, may contain Turkey calicivirus other viruses Not correct, Porcine Porcine parvovirus 6, respiratory Porcine circovirus 2, 218 APHA 6 Pig Lung Blinded Not analyzed yet ! and porcine circovirus 12a, reproductive porcine circovirus 1 syndrome virus (PRRSV) Correct, Bovine Cell 219 APHA 7 Cow Blinded Bovine respiratory syncytial virus Not analyzed yet ! Respiratory Supernatant Syncytial Virus Correct, Cell Bovine adenovirus A, 220 APHA 8 Cow Blinded Not analyzed yet ! Bovine Supernatant Adeno associated virus 5 Adenovirus Bovine parainfluenza virus 3, Correct, Cell Bovine viral diarrhea virus 1, Bovine PI3, 221 APHA 9 Cow Blinded Not analyzed yet ! Supernatant Pestivirus type 1, BVDV Simian Agent 10,

Porcine reovirus, Correct, Mammalian orthoreovirus type 2, Reovirus Cell 222 APHA 10 Cow? Blinded Mammalian orthoreovirus type 3, Not analyzed yet ! Supernatant Ndelle virus, Bovine parvovirus 1 Surveillance samples [Unknown - 223 APHA 11 Pig Faeces Blinded Porcine astrovirus 4 Not analyzed yet ! negative for Porcine Epidemic Diarrhoea ] Surveillance samples Porcine astrovirus 4, [Unknown - 224 APHA 12 Pig Faeces Blinded Not analyzed yet ! Porcine bocavirus negative for PED and TGE viruses] Surveillance Porcine stool associated circular samples virus, [Unknown - 225 APHA 13 Pig Faeces Blinded Not analyzed yet ! Porcine enterovirus, negative for Porcine Bastrovirus PED and TGE viruses] Porcine bocavirus, Surveillance Po Circo like virus 21, samples Porcine stool associated circular [Unknown - 226 APHA 14 Pig Faeces Blinded virus, Not analyzed yet ! negative for PoSCV Kor J481, PED and TGE (Porcine astrovirus), viruses] (Adeno associated virus) Porcine stool associated circular Surveillance virus 7, samples Porcine enterovirus, [Unknown - Porcine bocavirus, negative for 227 APHA 15 Pig Faeces Blinded Astrovirus wild boar, Not analyzed yet ! PED and TGE Porcine astrovirus 4, viruses] Gorilla smacovirus, Fur seal faeces associated circular DNA virus, (Picobirnavirus) Unknown negative for 228 APHA 16 Pig Heart Blinded Porcine circovirus Not analyzed yet ! porcine parvovirus Unknown Negative in 229 APHA 17 Duck Liver Blinded Goose parvovirus Not analyzed yet ! pan herpesvirus PCR African swine fever virus, Correct, 230 INIA 1.2 pig lung Blinded Torque teno sus virus k2 No PCR at SSI! African swine fever virus Pestivirus type 2, Correct, Hog cholera virus, Classical 231 INIA 2.2 pig serum Blinded Classical swine fever virus, Torque No PCR at SSI! swine fever teno sus virus k2, Torque teno sus virus 1a Porcine teschovirus 4, Correct, Porcine teschovirus 8, Porcine Porcine teschovirus 2, teschovirus 232 INIA 3.2 pig faeces Blinded Porcine teschovirus 1, No PCR at SSI! 12 (strain not Porcine enterovirus, included in Porcine bocavirus, array)) (Porcine adenovirus type 3) Peste des petits ruminants virus, Correct, Peste Ovine enzootic nasal tumor virus, des petits mesenteric 233 INIA 4.2 sheep Blinded Jaagsiekte sheep retrovirus, No PCR at SSI! ruminants ganglion Enzootic nasal tumor virus ofgoats, virus Adenovirus associated virus 5

Bluetongue virus 1 (5 segments), Correct, 234 INIA 5.2 sheep blood Blinded Bluetongue virus 4 (5 segments) No PCR at SSI! Bluetongue virus 4 Usutu virus, Correct, Avian Sarcomavirus, Usutu virus 235 INIA 6.2 grey partridge heart Blinded Not analyzed yet ! Avian myelocytomatosis virus Avian leukemia virus Usutu virus, Correct, Avian Sarcomavirus, Usutu virus 236 INIA 7.2 grey partridge kidney Blinded Not analyzed yet ! Avian myelocytomatosis virus Avian leukemia virus Spleen focus forming virus, Murine Not correct, type C retrovirus, ¨ Koutango Murine osteosarcoma virus, virus (WNV 237 INIA 8.2 mouse brain Blinded Murine leukemia virus, No PCR at SSI! putative Mouse mammary tumor virus, lineage 7) Exogenous mouse mammary tumor virus Spleen focus forming virus, Murine Not correct, type C retrovirus, WNV putative Murine osteosarcoma virus, lin 6, Malaysia 238 INIA 9.2 mouse brain Blinded Murine leukemia virus, No PCR at SSI! Mouse mammary tumor virus, Exogenous mouse mammary tumor virus, Bagaza virus, Correct, red-legged 239 INIA 10.2 feather pulp Blinded Israel turkey No PCR at SSI! Bagaza virus partridge meningoencephalomyelitis virus, West Nile virus lin 2, Correct, WNV Spleen focus forming virus, Murine lin 2 240 INIA 11.2 mouse brain Blinded type C retrovirus, Not analyzed yet ! Murine leukemia virus, Moloney murine leukemia virus red-legged West Nile virus lin 2 Correct, WNV 241 INIA 12.2 feather pulp Blinded Not analyzed yet ! partridge lin 2 West Nile virus lin 1, Correct, WNV 242 INIA 13.2 horse brain Blinded Not analyzed yet ! Kunjin virus lin 1 Negative Not correct, 243 INIA 14.2 golden eagle kidney Blinded Not analyzed yet ! WNV lin 1 cinereous West Nile virus linage 1, Correct, WNV 244 INIA 15.2 feather pulp Blinded Not analyzed yet ! vulture Kunjin virus lin 1 Negative No virus was 245 INIA 16.2 Barn owl brain Blinded identified previously Negative No virus was 246 INIA 17.2 Barn owl kidney Blinded identified previously Negative No virus was 247 INIA 18.2 Barn owl heart Blinded identified previously Negative No virus was 248 INIA 19.2 Barn owl spleen Blinded identified previously Negative No virus was 249 INIA 20.2 Barn owl lung Blinded identified previously Not European Betacoron 250 UoS 1 Faeces Negative Not analyzed yet ! confirmed by hedgehog avirus Owner MERS-like coronavirus, Not European Betacoron 251 UoS 2 Faeces Rotavirus A (cross hybridisation to Not analyzed yet ! confirmed by hedgehog avirus many species) Owner Not European Betacoron 252 UoS 3 Faeces Negative Not analyzed yet ! confirmed by hedgehog avirus Owner Not European 253 UoS 4 Faeces Unknown Negative Not analyzed yet ! confirmed by hedgehog Owner

Hedgehog hepatovirus, Not European Distal Large Betacoron 254 UoS 5 Alphaherpesvirus 1 (cross Not analyzed yet ! confirmed by hedgehog intestine avirus hybridisation to many species) Owner Not European Distal Large Betacoron 255 UoS 6 Negative Not analyzed yet ! confirmed by hedgehog intestine avirus Owner Not 256 UoS 7 Calf 6 liver Blinded Negative Not analyzed yet ! confirmed by Owner Not 257 UoS 8 Calf 6 trachea Blinded Negative Not analyzed yet ! confirmed by Owner Not 258 UoS 9 Cow 3 liver Blinded Negative Not analyzed yet ! confirmed by Owner Not 259 UoS 10 Cow 3 Lymph node Blinded Negative Not analyzed yet ! confirmed by Owner Not 260 UoS 11 Cow 4 liver Blinded Negative Not analyzed yet ! confirmed by Owner Not 261 UoS 12 Cow 4 Lymph node Blinded Negative Not analyzed yet ! confirmed by Owner Not 262 UoS 13 Cow 2 Lymph node unknown Negative Not analyzed yet ! confirmed by Owner Not 263 UoS 14 Cow 2 liver unknown Negative Not analyzed yet ! confirmed by Owner Not respiratory 264 UoS 15 Cow 2 unknown Negative Not analyzed yet ! confirmed by epithelium Owner Not gastrointesti 265 UoS 16 Cow 2 unknown Negative Not analyzed yet ! confirmed by ngal tract Owner Not Gastrointesti 266 UoS 17 Cow 3 unknown Lesavirus 1 Not analyzed yet ! confirmed by nal tract Owner Not respiratory 267 UoS 18 Cow 3 unknown Negative Not analyzed yet ! confirmed by epithelium Owner Not Gastrointesti 268 UoS 19 Cow 4 unknown Bovine astrovirus Not analyzed yet ! confirmed by nal tract Owner Not respiratory 269 UoS 20 Cow 4 unknown Salivirus Not analyzed yet ! confirmed by epithelium Owner Turkey hepatitis virus, Chicken rotavirus D, Chicken picornavirus 5, Chicken picornavirus 4, Chicken megrivirus, Not IZSLER intestinal Chicken astrovirus, 270 Chicken Blinded Not analyzed yet ! confirmed by 1.2 content Avian myelocytomatosis virus, Owner Avian leukosis virus, Avian leukemia virus, Caribou feces associated gemycircularvirus, Rous sarcoma virus Sapovirus pig sav1, Sapovirus pig Gansu, Porcine teschovirus, Porcine sapovirus, Not IZSLER intestinal 271 Pig Blinded Porcine rotavirus C, Not analyzed yet ! confirmed by 2.2 content Porcine rotavirus A, Owner Porcine kobuvirus, Porcine enterovirus, Porcine enterovirus 9,

Porcine enteric sapovirus, Porcine astrovirus 2, Pseudocowpox virus, skin Not IZSLER Parapoxvirus red deer, 272 Goat ulcerated Blinded Not analyzed yet ! confirmed by 3A.2 Orf virus, lesion Owner Bovine papular stomatitis virus Pseudocowpox virus, skin Not IZSLER Parapoxvirus red deer, 273 goat ulcerated Blinded Not analyzed yet ! confirmed by 3B.2 Orf virus, lesion Owner Bovine papular stomatitis virus skin Not IZSLER Bovine papillomavirus 5, 274 bovine proliferative Blinded Not analyzed yet ! confirmed by 4A.2 Bovine papillomavirus 7 lesion Owner skin Not IZSLER Bovine papillomavirus 5, 275 bovine proliferative Blinded Not analyzed yet ! confirmed by 4B.2 Bovine papillomavirus 7 lesion Owner Not IZSLER Rabbit hemorrhagic disease virus, 276 rabbit Liver Blinded Not analyzed yet ! confirmed by 5A.2 Rabbit calicivirus MRCV Owner Not IZSLER Rabbit hemorrhagic disease virus, 277 rabbit Liver Blinded Not analyzed yet ! confirmed by 5B.2 Rabbit calicivirus MRCV Owner Not IZSLER Rabbit hemorrhagic disease virus, 278 rabbit Liver Blinded Not analyzed yet ! confirmed by 5C.2 Rabbit calicivirus MRCV Owner Not IZSLER whole 279 bee Blinded Not analyzed yet ! Not analyzed yet ! confirmed by 6A.2 carcass Owner Not IZSLER whole 280 bee Blinded Not analyzed yet ! Not analyzed yet ! confirmed by 6B.2 carcass Owner Not IZSLER whole 281 bee Blinded Not analyzed yet ! Not analyzed yet ! confirmed by 6C.2 carcass Owner Turkey coronavirus, Sicinivirus 1, Rous sarcoma virus, Pigeon paramyxovirus 1, Newcastle disease virus, Infectious bursal disease virus, Fowl adenovirus 9, Not 282 IZSLER intestinal Fowl adenovirus 2, chicken Blinded Not analyzed yet ! confirmed by 7.2 content Duck coronavirus ZZ2004, Owner Chicken orivirus 1, Chicken megrivirus, Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus, Avian infectious bronchitis virus, Avian adeno associated virus Pigeon avian nephritis virus, Rous sarcoma virus, Chicken astrovirus, Not IZSLER intestinal 283 chicken Blinded Avian nephritis virus 2, Not analyzed yet ! confirmed by 8.2 content Avian myelocytomatosis virus, Owner Avian leukosis virus, Avian leukemia virus Posavirus 1, Porcine stool associated circular virus 1, Porcine kobuvirus, Not IZSLER Porcine epidemic diarrhea virus, 284 pig faecal swabs Blinded Not analyzed yet ! confirmed by 9.2 Porcine enterovirus, Owner Porcine bocavirus, Porcine astrovirus 4, Po Circo like virus 21, Astrovirus wild boar Turkey rotavirus A, Not IZSLER intestinal 285 turkey Blinded Turkey astrovirus 3, Not analyzed yet ! confirmed by 10.2 content Turkey astrovirus 2, Owner

Turkey astrovirus 1, Chicken stool-associated gemycircularvirus, Caribou feces associated gemycircularvirus IZSLER whole 286 bee Blinded Not analyzed yet ! Not analyzed yet ! 11A.2 carcass IZSLER whole 287 bee Blinded Not analyzed yet ! Not analyzed yet ! 11B.2 carcass IZSLER whole 288 bee Blinded Not analyzed yet ! Not analyzed yet ! 11C.2 carcass Pseudocowpox virus, Not IZSLER skin lesion Parapoxvirus red deer, 289 roe deer Blinded Not analyzed yet ! confirmed by 12A.2 (crusts) Orf virus, Bovine papular Owner stomatitis virus Ovine enzootic nasal tumour virus, Orf virus, Not IZSLER skin lesion 290 roe deer Blinded Jaagsiekte sheep retrovirus, Not analyzed yet ! confirmed by 12B.2 (crusts) Enzootic nasal tumour virus of Owner goats, Pseudocowpox virus Sicinivirus 1, Infectious bursal disease virus, Fowl adenovirus 9, Fowl adenovirus 2, Fowl adenovirus 11, Not IZSLER intestinal 291 chicken Blinded Fowl adenovirus 3, Not analyzed yet ! confirmed by 13.2 content Chicken stool-associated Owner gemycircularvirus, Chicken rotavirus G, Chicken orivirus 1, Chicken astrovirus Gallivirus Pf CHK1 GV, Fowl adenovirus 9, Fowl adenovirus 2, Fowl adenovirus 3, Not IZSLER intestinal 292 chicken Blinded Fowl adenovirus 11, Not analyzed yet ! confirmed by 14.2 content Chicken gallivirus 1, Owner Chicken anemia virus, Avian leukemia virus, Avian adeno associated virus cellular Not IZSLER culture 293 bat Blinded Bat adenovirus 2 Not analyzed yet ! confirmed by 15A.2 (5°pass. Owner VERO) cellular Not IZSLER culture 294 bat Blinded Bat adenovirus 2 Not analyzed yet ! confirmed by 15B.2 (5°pass. Owner VERO) Not IZSLER Porcine rotavirus B, 295 pig faeces Blinded Not analyzed yet ! confirmed by 16.2 Porcine kobuvirus Owner Turkey rotavirus A, Turkey astrovirus 3, Turkey astrovirus 2, Not IZSLER intestinal 296 turkey Blinded Turkey astrovirus 1, Not analyzed yet ! confirmed by 17.2 content Duck associated cyclovirus 1, Owner Caribou feces associated gemycircularvirus Rous sarcoma virus, Duck associated cyclovirus 1, Not IZSLER pool of 297 chicken Blinded Avian myelocytomatosis virus, Not analyzed yet ! confirmed by 18A.2 organs Avian leukosis virus, Owner Avian leukemia virus Rous sarcoma virus, Duck associated cyclovirus 1, Not IZSLER pool of 298 chicken Blinded Avian myelocytomatosis virus, Not analyzed yet ! confirmed by 18B.2 organs Avian leukosis virus, Owner Avian leukemia virus,

Caribou feces associated gemycircularvirus, chicken parvovirus Porcine torovirus, Newbury agent 1 virus, Nebovirus, Human picobirnavirus, Calicivirus TCG, Calicivirus NB, Breda virus, Not IZSLER intestinal 299 bovine Blinded Bovine kobuvirus, Not analyzed yet ! confirmed by 19.2 content Bovine enteric calicivirus, Owner Bovine adenovirus type B, Not confirmed by Owner Bovine adeno associated virus, Aichivirus B, Adeno associated virus 5 Not IZSLER insect 300 carcasses Blinded Adana virus Not analyzed yet ! confirmed by 20A.2 (sandflies) Owner Not IZSLER insect Adana virus, 301 carcasses Blinded Not analyzed yet ! confirmed by 20B.2 (sandflies) Medjerda valley virus Owner IZSLER 302 cricket carcasses Blinded Not analyzed yet ! Not analyzed yet ! 21.2 Not IZSLER pool of 303 duck Blinded negativ Not analyzed yet ! confirmed by 22A.2 organs Owner Not IZSLER pool of (Duck picornavirus), 304 duck Blinded Not analyzed yet ! confirmed by 22B.2 organs Cyclovirus NG12 Owner Y73 sarcoma virus, UR2 sarcoma virus, Turkey stool associated circular ssDNA virus, Turkey hepatitis virus, Rous sarcoma virus, Melegrivirus A, Not IZSLER intestinal 305 chicken Blinded Chicken picornavirus 5, Not analyzed yet ! confirmed by 23.2 content Chicken picornavirus 4, Owner Chicken megrivirus, Chicken astrovirus, Avian Sarcoma Virus, Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus, Y73 sarcoma virus, UR2 sarcoma virus, Rous sarcoma virus, Gallid herpesvirus 1, Not IZSLER 306 chicken trachea Blinded Chicken anemia virus, Not analyzed yet ! confirmed by 24A.2 Avian Sarcoma Virus, Owner Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus Y73 sarcoma virus, UR2 sarcoma virus, Rous sarcoma virus, Gallid herpesvirus 1, Not IZSLER Chicken anemia virus, 307 chicken trachea Blinded Not analyzed yet ! confirmed by 24B.2 Avian Sarcoma Virus, Owner Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus, Avian carcinoma virus Y73 sarcoma virus, Rous sarcoma virus, Not IZSLER Gallid herpesvirus 1, 308 chicken l. allantoid. Blinded Not analyzed yet ! confirmed by 25A.2 Avian Sarcoma Virus, Owner Avian myelocytomatosis virus, Avian leukosis virus,

Avian leukemia virus Y73 sarcoma virus, Rous sarcoma virus, Gallid herpesvirus 1, Not IZSLER Avian Sarcoma Virus, 309 chicken l. allantoid. Blinded Not analyzed yet ! confirmed by 25B.2 Avian myelocytomatosis virus, Owner Avian leukosis virus, Avian leukemia virus, Chicken anemia virus Not IZSLER 310 bovine skin Blinded (Herpesvirus) Not analyzed yet ! confirmed by 26A.2 Owner Not IZSLER 311 bovine skin Blinded (Herpesvirus) Not analyzed yet ! confirmed by 26B.2 Owner cellular Not IZSLER culture 312 bat Blinded Eptesipox virus Not analyzed yet ! confirmed by 27A.2 (2°pass. Owner VERO) cellular Not IZSLER culture 313 bat Blinded Negative Not analyzed yet ! confirmed by 27B.2 (2°pass. Owner VERO) cellular Not IZSLER culture 314 bat Blinded Negative Not analyzed yet ! confirmed by 28A.2 (3°pass. Owner VERO) cellular Not IZSLER culture 315 bat Blinded Negative Not analyzed yet ! confirmed by 28B.2 (3°pass. Owner VERO) Kobuvirus cattle Kagoshima 2, Porcine torovirus, Breda virus, Not PIWET faecal 316 cattle Blinded Bovine parvovirus 1, Not analyzed yet ! confirmed by 1.2 suspension Bovine kobuvirus, Owner Aichivirus B, (Bovine astrovirus) Porcine stool associated circular virus 7, Bovine enterovirus, Not PIWET faecal 317 cattle Blinded Bovine kobuvirus, Not analyzed yet ! confirmed by 2.2 suspension Aichivirus B, Owner Caribou feces associated gemycircularvirus Not PIWET faecal Caribou feces associated 318 cattle Blinded Not analyzed yet ! confirmed by 3.2 suspension gemycircularvirus Owner Porcine teschovirus, Porcine teschovirus 1, Porcine teschovirus 10, Porcine stool associated circular virus, Porcine sapelovirus 1, Not PIWET faecal Porcine kobuvirus, 319 pig Blinded Not analyzed yet ! confirmed by 4.2 suspension Porcine enterovirus, Owner Porcine enterovirus 8, Porcine bocavirus, Porcine astrovirus 4, Caribou feces associated gemycircularvirus, Astrovirus wild boar Posavirus 1, Porcupine astrovirus, Porcine teschovirus 1-11, Not PIWET faecal 320 pig Blinded Porcine stool associated circular Not analyzed yet ! confirmed by 5.2 suspension virus 7, Owner Porcine stool associated circular virus 3,

Porcine stool associated circular virus 2, Porcine stool associated circular virus 1, Porcine enterovirus B, Porcine enterovirus 9, Porcine enterovirus 8, Porcine enterovirus 1, Porcine circovirus 2, Porcine bocavirus, Porcine bocavirus 5, Porcine bocavirus 4, Porcine bocavirus 3, Porcine bocavirus 2, Porcine astrovirus 4, Porcine astrovirus 2, Picornavirales Tottori HG1, Fur seal faeces associated circular DNA virus, Astrovirus wild boar Porcine rotavirus C (10 segments,no segment 9), Not PIWET faecal 321 pig Blinded Porcine rotavirus A (10 segments, Not analyzed yet ! confirmed by 6.2 suspension no segment 4), Owner Porcine kobuvirus Posavirus 1, Porcine stool associated circular virus 4, Porcine stool associated circular virus 7, Porcine kobuvirus, Not PIWET faecal 322 pig Blinded Porcine enterovirus B, Not analyzed yet ! confirmed by 7.2 suspension Porcine enterovirus 9, Owner Porcine astrovirus 4, Caribou feces associated gemycircularvirus, Astrovirus wild boar, (Swine pasivirus 1) Swine Hepatitis E virus, Posavirus 1, Porcine stool associated circular virus 7, Not PIWET faecal 323 pig Blinded Hepatitis E virus wildlife, Hepatitis Not analyzed yet ! confirmed by 8.2 suspension E virus genotype 3, Owner Fur seal faeces associated circular DNA virus, Enterovirus G Sapovirus pig sav1, Sapovirus pig Gansu, Posavirus 1, Porcine teschovirus, Porcine stool associated circular virus 7, Porcine sapovirus, Porcine sapelovirus 1, Porcine rotavirus C, Porcine rotavirus A, Porcine kobuvirus, Not PIWET faecal Porcine enterovirus G1, 324 pig Blinded Not analyzed yet ! confirmed by 9.2 suspension Porcine enterovirus B, Owner Porcine enterovirus 8, Porcine enterovirus 10, Porcine enterovirus 3H, Porcine enteric sapovirus, Porcine bocavirus 3, Porcine bocavirus 4, Porcine bocavirus 5, Porcine astrovirus 2, Porcine astrovirus 4, Po Circo like virus 51, Astrovirus wild boar

Swine pasivirus 1, Porcine stool associated circular virus 7, Posavirus sp, Porcine enterovirus 9, Porcine enterovirus 3H, Not PIWET faecal Porcine enterovirus 15, 325 pig Blinded Not analyzed yet ! confirmed by 10.2 suspension Porcine enterovirus 10, Owner Porcine bocavirus 4, Porcine astrovirus 4, Pasivirus A, Parechovirus like virus, Astrovirus wild boar, Adeno associated virus Porcine kobuvirus, Human picobirnavirus, Porcine stool associated circular Not PIWET faecal 326 pig Blinded virus 7, Not analyzed yet ! confirmed by 11.2 suspension Porcine stool associated circular Owner virus 2, Porcine rotavirus A Kobuvirus cattle Kagoshima 2, Not PIWET faecal Breda virus, 327 pig Blinded Not analyzed yet ! confirmed by 12.2 suspension Porcine torovirus, Owner Bovine parvovirus 1 Porcine astrovirus 4, Astrovirus wild boar, Bastrovirus Porcine, Not PIWET faecal 328 pig Blinded Porcine enterovirus, Not analyzed yet ! confirmed by 13.2 suspension Porcine stool associated circular Owner virus 7, Porcine bocavirus 4 Not PIWET faecal Porcine kobuvirus, 329 pig Blinded Not analyzed yet ! confirmed by 14.2 suspension Caprine kobuvirus Owner Not PIWET faecal 330 rabbit Blinded negativ Not analyzed yet ! confirmed by 15.2 suspension Owner Not PIWET Torque teno sus virus 1b, 331 wild boar serum Blinded Not analyzed yet ! confirmed by 16.2 Swine hepatitis E virus Owner Torque teno sus virus 1a, Torque teno sus virus 1b, Torque teno sus virus k2, Not PIWET 332 wild boar serum Blinded Porcine parvovirus 1, Not analyzed yet ! confirmed by 17.2 Porcine circovirus 2, Owner Porcine circovirus 12a, Porcine circovirus 2 Not PIWET Swine Hepatitis E virus, 333 wild boar serum Blinded Not analyzed yet ! confirmed by 16.2 Torque teno sus virus 1b Owner Swine Hepatitis E virus, Not PIWET liver 334 wild boar Blinded Porcine circovirus 2, Not analyzed yet ! confirmed by 19.2 homogenate Porcine circovirus 12 Owner Not PIWET liver Swine Hepatitis E virus, 335 wild boar Blinded Not analyzed yet ! confirmed by 20.2 homogenate Torque teno sus virus 1b Owner Fowl adenovirus D, Not PIWET spleen Fowl adenovirus 9, 336 chicken Blinded Not analyzed yet ! confirmed by 21.2 homogenate Fowl adenovirus 2, Owner Fowl adenovirus 11 Fowl adenovirus D, Fowl adenovirus 9, Not PIWET liver Fowl adenovirus 3, 337 chicken Blinded Not analyzed yet ! confirmed by 22.2 homogenate Fowl adenovirus 2, Owner Fowl adenovirus 11, Avian leukemia virus Rous sarcoma virus, Not PIWET liver 338 chicken Blinded Chicken Parvovirus, Not analyzed yet ! confirmed by 23.2 homogenate Avian Sarcoma virus, Owner

Avian myelocytomatosis, Avian leukosis virus, Avian leukemia virus, Y73 sarcoma virus Y73 sarcoma virus, UR2 sarcoma virus, Turkey hepatitis virus, Rous sarcoma virus, Melagrivirus A, Human gyrovirus, Chicken rotavirus D, Not PIWET liver Chicken picornavirus 5, 339 chicken Blinded Not analyzed yet ! confirmed by 24.2 homogenate Chicken picornavirus 4, Owner Chicken megrivirus, Chicken astrovirus, avian Sarcoma Virus, Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus, avian gyrovirus Y73 sarcoma virus, Rous sarcoma virus, Infectious bursal disease virus, Human gyrovirus, Fowl adenovirus D, Fowl adenovirus 9, Not PIWET gizzard Fowl adenovirus 2, 340 chicken Blinded Not analyzed yet ! confirmed by 25.2 homogenate Fowl adenovirus 11, Owner Chicken parvovirus, Avian sarcoma virus, Avian myelocytomatosis virus, Avian leukosis virus, Avian leukemia virus, Avian gyrovirus WNV Fr15 WNV lin1, Correct, WNV 341 ANSES 9 isolate Blinded Not analyzed yet ! isolate Kunjin virus lin 1a ANSES Not correct, 342 Caballus equi brain Blinded negativ Not analyzed yet ! 10 WNV lin 1a ANSES Not correct, 343 Caballus equi brain Blinded negativ Not analyzed yet ! 10 WNV lin 1a ANSES Correct, WNV 344 Buteo buteo brain Blinded WNV lin2 Not analyzed yet ! 11 lin 2 ANSES Correct, USUV 345 Turdus merula brain Blinded Usutu virus Not analyzed yet ! 12 Europe3 Not correct, ANSES 346 Caballus equi brain Blinded negativ No PCR at SSI ! Equine herpes 13 virus 2 Not correct, ANSES 347 Caballus equi brain Blinded negativ No PCR at SSI ! Equine herpes 13 virus 2 No virus was ANSES 348 Caballus equi brain unknown negativ identified 14 previously No virus was ANSES 349 Gyps fulvus brain unknown negativ identified 15 previously No virus was ANSES Passer 350 brain unknown negativ identified 16 domesticus previously No virus was ANSES 351 Caballus equi brain unknown negativ identified 17 previously No virus was ANSES 352 Caballus equi brain unknown negativ identified 18 previously No virus was ANSES 353 Caballus equi brain unknown negativ identified 19 previously ANSES 354 EEEV isolate isolate Blinded Eastern equine encephalitis virus Not analyzed yet ! Correct, EEEV 20

No virus was ANSES 355 Caballus equi brain unknown negativ identified 21 previously No virus was ANSES 356 Caballus equi brain unknown negativ identified 22 previously No virus was ANSES 357 Caballus equi brain unknown negativ identified 23 previously No virus was ANSES 358 Caballus equi brain unknown Abelson murine leukemia virus Not analyzed yet ! identified 24 previously No virus was ANSES 359 Caballus equi brain unknown Abelson murine leukemia virus Not analyzed yet ! identified 24 previously No virus was ANSES 360 Caballus equi brain unknown negativ identified 25 previously Not tissue 361 IZSAM-1 HORSE Blinded African horse sickness virus Not analyzed yet ! confirmed by culture Owner Not tissue Epizootic hemorrhagic disease 362 IZSAM-2 DEER Blinded Not analyzed yet ! confirmed by culture virus 1 Owner Not tissue Canine mobillivirus, 363 IZSAM-3 DOG Blinded Not analyzed yet ! confirmed by culture Canine distemper virus Owner Not tissue Bluetongue virus type 4 (cross 364 IZSAM-4 SHEEP Blinded Not analyzed yet ! confirmed by culture hybridization between many types) Owner Not tissue Bluetongue virus type 3 (cross 365 IZSAM-5 SHEEP Blinded Not analyzed yet ! confirmed by culture hybridization between many types) Owner Not tissue 366 IZSAM-6 BIRD Blinded West Nile virus lin 1, Kunjin virus Not analyzed yet ! confirmed by culture Owner Not tissue 367 IZSAM-7 BIRD Blinded West Nile virus lin 2 Not analyzed yet ! confirmed by culture Owner Pigeonpox virus, Penguinpox virus, Fowlpox virus, Flamingopox Not reference tissue 368 IZSAM-8 Blinded virus, Avian myelocytomatosis Not analyzed yet ! confirmed by strain culture virus, Avian leukosis virus, Avian Owner leukemia virus Not tissue 369 IZSAM-9 BIRD Blinded Usutu virus Not analyzed yet ! confirmed by culture Owner Ovine enzootic nasal tumour Not IZSAM- virus, Jaagsiekte sheep retrovirus, 370 SHEEP Whole blood Blinded Not analyzed yet ! confirmed by 10 Enzootic nasal tumour virus of Owner goats Ovine enzootic nasal tumour Not IZSAM- virus, Jaagsiekte sheep retrovirus, 371 GOAT Whole blood Blinded Not analyzed yet ! confirmed by 11 Enzootic nasal tumour virus of Owner goats Ovine enzootic nasal tumour virus, Not IZSAM- 372 CATTLE Whole blood Blinded Jaagsiekte sheep retrovirus, Not analyzed yet ! confirmed by 12 Enzootic nasal tumour virus of Owner goats supernatant Not IZSAM- Usutu virus, 373 BIRD of organs Blinded Not analyzed yet ! confirmed by 13 (West Nile virus) homogenate Owner supernatant Not IZSAM- 374 BIRD of brain Blinded negativ Not analyzed yet ! confirmed by 14 homogenate Owner supernatant Not IZSAM- 375 WILD BOAR of intestine Blinded negativ Not analyzed yet ! confirmed by 15 homogenate Owner

supernatant Canine circovirus, Canine Not IZSAM- 376 WOLF of spleen Blinded mobillivirus, Torque teno canis Not analyzed yet ! confirmed by 16 homogenate virus, Canine distemper virus Owner supernatant Not IZSAM- of limph Canine circovirus, 377 WOLF Blinded Not analyzed yet ! confirmed by 17 nodes (canine picornavirus) Owner homogenate supernatant Not IZSAM- 378 WOLF of brain Blinded negativ Not analyzed yet ! confirmed by 18 homogenate Owner supernatant Not IZSAM- 379 OTTER of lung Blinded negativ Not analyzed yet ! confirmed by 19 homogenate Owner RD114 retrovirus, Not IZSAM- tissue Pestivirus type 1, 380 CAT Blinded Not analyzed yet ! confirmed by 20 culture Feline morbillivirus, Owner Bovine viral diarrhea virus 1 2018 EVD- YFV RT-PCR 381 SSI 44 LabNet YF plasma 5,4x103 Yellow fever virus Correct, YFV 37/38 panel copies 2018 EVD- YFV RT-PCR 382 SSI 45 LabNet YF plasma: 3,54x104 Yellow fever virus Correct, YFV 33/22 panel copies 2018 EVD- YFV RT-PCR 383 SSI 46 LabNet YF plasma 3,4x103 Yellow fever virus Correct, YFV 36/27 panel copies Zika virus, Zika RT-PCR West Nile virus 1, 27/13 Parainfluenza virus 5, WNV RT-PCR Zika virus, 2018 EVD- Kunjin virus, 26/10 WNV, Correct, WNV, 383 SSI 47 LabNet YF Plasma DENV 4, DENV RT-PCR Dengue DENV, Zika panel DENV 3, 27/19 virus DENV2, Usutu RT-PCR DENV 1, 34/31 Usutu virus Could not be 2018 EVD- YFV RT-PCR confirmed by 384 SSI 48 LabNet YF urine 3,54x105 Negativ No ct/no ct PC = PNA panel copies degraded 2018 EVD- YFV Rt-PCR 385 SSI 49 LabNet YF plasma 4,7x106 Yellow fever virus Correct, YFV 27/not tested panel copies 2018 EVD- Correct, YFV 386 SSI 50 LabNet YF Neg plasma Negative Negative negative panel 2018 EVD- YFV RT-PCR 387 SSI 51 LabNet YF plasma 2,8x106 Yellow fever virus Correct, YFV 24/7 panel copies 2018 EVD- YFV RT-PCR 388 SSI 52 LabNet YF plasma 2,8x105 Yellow fever virus Correct, YFV 28/18 panel copies 2018 EVD- Correct, YFV 389 SSI 53 LabNet YF neg urine Negativ Negative negative panel 2018 EVD- YFV RT-PCR 390 SSI 54 LabNet YF urine 2,3x103 Negative Correct, YFV No ct/no ct panel copies 2018 EVD- YFV RT-PCR 391 SSI 55 LabNet YF plasma 4,75x105 Yellow fever virus Correct, YFV 27/17 panel copies 2018 EVD- YFV RT-PCR 392 SSI 56 LabNet YF plasma 3,7x103 Yellow fever virus Correct, YFV 33/38 panel copies 2018 EVD- YFV RT-PCR 393 SSI 57 LabNet YF plasma 6,5x104 Yellow fever virus Correct, YFV 31/21 panel copies 2018 EVD- YFV RT-PCR 394 SSI 58 LabNet YF Plasma 4,9x104 Yellow fever virus Correct, YFV 30/35 panel copies

Toscana lin 2017 EVD- A LabNet Toscana virus, Correct, 395 SSI 59 plasma 1,75x105 Not analyzed yet ! neutropisk Chikungunya virus Toscana virus copies/0,4 panel ml Toscana lin 2017 EVD- B LabNet Correct, 396 SSI 60 plasma 1,24x105 (Toscana virus) Not analyzed yet ! neutropisk Toscana virus copies/0,4 panel ml 2017 EVD- TBE TBE, LabNet 5,06x104 Negishi virus, 397 SSI 61 plasma Not analyzed yet ! Correct, TBE neutropisk copies/0,4 Louping ill virus, panel ml Spanish goat encephalitis virus 2017 EVD- WNV lin 1 LabNet 1,2x104 WNV lin 1, Correct, WNV 398 SSI 62 plasma Not analyzed yet ! neutropisk copies/0,4 Kunjin virus lin 1 panel ml 2017 EVD- WNV lin 2 LabNet 4.96x105 Correct, WNV 399 SSI 63 plasma WNV lin 2 Not analyzed yet ! neutropisk copies/0,4 lin 2 panel ml 2017 EVD- Usutu LabNet 6.34x103 Correct, 400 SSI 64 plasma Usutu virus Not analyzed yet ! neutropisk copies/0,4 Usutu panel ml 2017 EVD- LabNet neg ctrl Correct, 401 SSI 65 Negativ Negativ Not analyzed yet ! neutropisk plasma negative panel 2017 EVD- LabNet neg ctrl Correct, 402 SSI 65 Negativ Negativ Not analyzed yet ! neutropisk plasma negative panel 2017 EVD- LabNet neg ctrl Correct, 403 SSI 67 Negativ Negativ Not analyzed yet ! neutropisk plasma negative panel 2017 EVD- LabNet neg ctrl Correct, 405 SSI 68 Negativ Negativ Not analyzed yet ! neutropisk plasma negative panel The simple should be HKU1 positive according to HKU1 Bovine viral diarrhea virus 2, Panel Corona RT-PCR company, 406 SSI 69 corona Border disease virus C413, 18S05 No ct/no ct however we virus Pestivirus type 1 can not confirm this by PCR, NGS or microarray! No virus was 407 SSI 70 Human Spinal Unknown Negative identified previously No virus was 408 SSI 71 Human EDTA-blod Unknown Negative identified previously No virus was 409 SSI 72 Human blood Unknown Negative identified previously No virus was 410 SSI 73 Human EDTA-blod Unknown Negative identified previously No virus was Spinal, 411 SSI 74 Human Unknown Negative identified serum previously No virus was Tracheal 412 SSI 75 Human Unknown Negative identified swabs previously

No virus was 413 SSI 76 Human plasma Unknown Negative identified previously No virus was 414 SSI 77 Human plasma Unknown Negative identified previously No virus was 415 SSI 78 Human plasma Unknown Negative identified previously No virus was 416 SSI 79 Human plasma Unknown Negative identified previously No virus was 417 SSI 80 Human plasma Unknown Torque teno mini virus Not analyzed yet ! identified previously No virus was 418 SSI 81 Human plasma Unknown Negative identified previously No virus was 419 SSI 82 Human plasma Unknown Negative identified previously No virus was 420 SSI 83 Human plasma Unknown Negative identified previously No virus was 421 SSI 84 Human plasma Unknown Negative identified previously No virus was 422 SSI 85 Human plasma Unknown Negative identified previously No virus was 423 SSI 86 Lemur, ZOO Fecal Unknown Negative identified previously No virus was 424 SSI 87 Lemur, ZOO Fecal Unknown Picornavirales Bu 1 No PCR at SSI ! identified previously No virus was 425 SSI 88 Lemur, ZOO Fecal Unknown Negative identified previously No virus was 426 SSI 89 Lemur, ZOO Fecal Unknown Negative identified previously 2018 EVD- YFV 427 SSI 90 LabNet YF Plasma 3,4x103 YFV Not analyzed yet ! Correct, YFV panel copies Human coronavirus OC43, Correct, QCMD-panel Bovine viral diarrhea virus 2, CVRNA17S- Coronaviru Human 428 SSI 91 (CVRNA17S- Bovine polyomavirus 1, Not analyzed yet ! 06 s - OC43 coronavirus 06) Bovine parvovirus 2, OC43 Border disease virus C413 Human rubulavirus 2, Correct, QCMD-panel Parainfluen Human parainfluenza virus 2, PINFRNA17S Human 429 SSI 92 (PINFRNA17S- z avirus Bovine viral diarrhea virus 2, Not analyzed yet ! - 10 parainfluenza 10) type 2 Bovine parvovirus 2, virus 2 Border disease virus C413 Pestivirus type 1, QCMD-panel Bovine viral diarrhea virus 2, PeVRNA17C Correct, 430 SSI 93 (PeVRNA17C1- HPeV4 Bovine viral diarrhea virus 1, Not analyzed yet ! 1- 04 Enterovirus 04) Border disease virus C413, Enterovirus Correct, QCMD-panel Metapneu Human 431 SSI 94 MPV16- 01 Human Metapneumovirus A Not analyzed yet ! (MPV16- 01) movirus A Metapneumo virus A Human adenovirus type 4, Human mastadenovirus E, Chimpanzee Correct, QCMD-panel ADVDNA16C ADV adenovirus Y25, Simian adenovirus Human 432 SSI 95 (ADVDNA16C1- Not analyzed yet ! 1- 02 type 4 type 25, Simian adenovirus type adenovirus 02) 26, Simian adenovirus type 30, type 4 Simian adenovirus type 37.2

Correct, QCMD-panel B19DNA17C B19 virus Human 433 SSI 96 (B19DNA17C2- Human parvovirus B19 Not analyzed yet ! 2- 03 type 1 parvovirus 03) B19 QCMD-panel Correct, CMVDNA17 CMV 434 SSI 97 (CMVDNA17C2 Human Herpesvirus 5 Not analyzed yet ! Human C2- 03 (Clinical) - 03) Herpesvirus 5 QCMD-panel Correct, HHV6 HHV6DNA17 HHV6 HHV6 type A, 435 SSI 98 (HHV6DNA17C Not analyzed yet ! type A C1- 03 type A HHV6 type B 1- 03) Correct, Human rhinovirus A90, QCMD-panel Human RVRNA17S- Rhinovirus Pestivirus type 1, 436 SSI 99 (RVRNA17S- Not analyzed yet ! rhinovirus 01 - 90 Bovine viral diarrhea virus 01) A90 Border disease virus C413

Correct, QCMD-panel Enterovirus D68, RVRNA17S- Enterovirus Enterovirus 437 SSI 100 (RVRNA17S- Bovine viral diarrhea virus 2, Not analyzed yet ! 05 68 D68 05) Border disease virus C413

Zika virus Zika virus, Correct, Zika QCMD-panel 438 SSI 101 ZIKA17S- 01 (French Bovine viral diarrhea virus 1, Not analyzed yet ! virus (ZIKA17S- 01) Polynesian) Pestivirus type 1 2018 EVD- YFV RT-PCR Correct, YF 439 SSI 102 LabNet YF Plasma 3,4x103 YFV 33,11/not virus panel copies analyzed 2018 EVD- YFV RT-PCR Correct, YF 440 SSI 103 LabNet YF urine (YF 5) 3,5x105 YFV 29,59/not virus panel copies analyzed 2018 EVD- RT-PCR Human papillomavirus KC5, Correct, YF 441 SSI 104 LabNet YF urine neg ctrl no ct (Human polyomavirus 7) virus negative panel 2018 EVD- YFV RT-PCR Not correct, 442 SSI 105 LabNet YF urine 2,3x103 (Human papillomavirus KC5) 36,08/not YF virus panel copies analyzed negative Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, PCR Correct, 443 SSI 106 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, 26,25/not Orthopox (1#) 2019 (8#) Ectromelia virus, Cowpox virus, analyzed positive Camelpox virus Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, PCR Correct, 444 SSI 107 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, 29,59/not Orthopox (2#) 2019 (8#) Ectromelia virus, Cowpox virus, analyzed positive Camelpox virus Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, Correct, PCR 445 SSI 108 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, Orthopox 29,9/not analyzed (3#) 2019 (8#) Ectromelia virus, Cowpox virus, positive Camelpox virus EVD-labNet EVD-labNet Correct, PCR 446 SSI 109 Pox panel 2019 Pox panel Orthopox negative Orthopox no ct (4#) 2019 (8#) negative Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, PCR Correct, 447 SSI 110 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, 28,21/not Orthopox (5#) 2019 (8#) Ectromelia virus, Cowpox virus, analyzed positive Camelpox virus Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, PCR Correct, 448 SSI 111 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, 34,08/not Orthopox (6#) 2019 (8#) Ectromelia virus, Cowpox virus, analyzed positive Camelpox virus Variola virus, Vaccinia virus, EVD-labNet EVD-labNet Taterapox virus, Rabbitpox virus, PCR Correct, 449 SSI 112 Pox panel 2019 Pox panel Orthopox Monkeypox virus, Horsepox virus, 34,59/not Orthopox (7#) 2019 (8#) Ectromelia virus, Cowpox virus, analyzed positive Camelpox virus EVD-labNet EVD-labNet PCR Correct, 450 SSI 113 Pox panel 2019 Pox panel Orthopox Orf virus, (Pseudocowpox virus) 28,97/not Orthopox (8#) 2019 (8#) analyzed positive

Supplementary Table 5: 3rd round at SSI

PCR confirmation Virus ct-values Confirmation No Name Species Material Array result content before/after by owner WTA No virus was VRI Erinaceus Spleen 451 Unknown Negative identified 1/2019 europaeus homogenate previously No virus was VRI Erinaceus brain 452 Unknown Negative identified 2/2019 europaeus homogenate previously No virus was VRI Erinaceus Spleen 453 Unknown Negative identified 3/2019 europaeus homogenate previously No virus was VRI Erinaceus brain 454 Unknown Negative identified 4/2019 europaeus homogenate previously No virus was VRI Erinaceus rou Spleen (Salivirus NGJ1, Porcine circovirus 455 Unknown identified 5/2019 manicus homogenate 2) previously No virus was VRI Erinaceus rou brain 456 Unknown Negative identified 6/2019 manicus homogenate previously No virus was VRI Spleen 457 Sciurus vulgaris Unknown Negative identified 7/2019 homogenate previously No virus was VRI8/20 brain 458 Sciurus vulgaris Unknown Negative identified 19 homogenate previously No virus was VRI Spleen 459 Sciurus vulgaris Unknown Negative identified 9/2019 homogenate previously No virus was VRI brain 460 Sciurus vulgaris Unknown Negative identified 10/2019 homogenate previously No virus was VRI liver 461 Turdus merula Unknown (Simian adenovirus) identified 11/2019 homogenate previously No virus was VRI brain 462 Turdus merula Unknown Negative identified 12/2019 homogenate previously No virus was VRI liver 463 Turdus merula Unknown Not analysed yet! identified 13/2019 homogenate previously No virus was VRI brain 464 Turdus merula Unknown Not analysed yet! identified 14/2019 homogenate previously No virus was VRI Erinaceus rou Spleen 465 Unknown Not analysed yet! identified 15/2019 manicus homogenate previously No virus was VRI Erinaceus rou brain 466 Unknown Not analysed yet! identified 16/2019 manicus homogenate previously No virus was VRI Nyctalus liver 467 Unknown Not analysed yet! identified 17/2019 noctula homogenate previously No virus was VRI Nyctalus lung 468 Unknown Not analysed yet! identified 18/2019 noctula homogenate previously No virus was VRI Nyctalus kidney 469 Unknown Not analysed yet! identified 19/2019 noctula homogenate previously No virus was VRI Eptesicus kidney 470 Unknown Not analysed yet! identified 20/2019 nilssonii homogenate previously

No virus was VRI Eptesicus liver 471 Unknown Not analysed yet! identified 21/2019 nilssonii homogenate previously No virus was VRI Myotis kidney 472 Unknown Not analysed yet! identified 22/2019 daubentonii homogenate previously No virus was VRI Myotis liver 473 Unknown Not analysed yet! identified 23/2019 daubentonii homogenate previously No virus was VRI Nyctalus kidney 474 Unknown Not analysed yet! identified 24/2019 noctula homogenate previously No virus was VRI Nyctalus liver 475 Unknown Not analysed yet! identified 25/2019 noctula homogenate previously Vaccinia virus, Horsepox virus, PCR EVD-labNet EVD-labNet Rabbitpox virus, Cowpox virus, 29,71/not Correct, 476 SSI 114 Pox panel 2019 Pox panel Orthopox Variola virus, Taterapox virus, analyzed Orthopox (9#) 2019 (9#) camelpox virus, Ectomelia virus, positive Monkey pox Vaccinia virus, Horsepox virus, PCR EVD-labNet EVD-labNet Rabbitpox virus, Cowpox virus, 27,34/not Correct, 477 SSI 115 Pox panel 2019 Pox panel OrthopoX Variola virus, Taterapox virus, analyzed Orthopox (10#) 2019 (10#) camelpox virus, Ectomelia virus, positive Monkey pox EVD-labNet EVD-labNet no ct Correct, 478 SSI 116 Pox panel 2019 Pox panel Orthopox Human herpesvirus 1 Orthopox (11#) 2019 (11#) negative Vaccinia virus, Horsepox virus, PCR EVD-labNet EVD-labNet Rabbitpox virus, Cowpox virus, 28,73/not Correct, 479 SSI 117 Pox panel 2019 Pox panel Orthopox Variola virus, Taterapox virus, analyzed Orthopox (12#) 2019 (12#) camelpox virus, Ectomelia virus, positive Monkey pox EVD-labNet EVD-labNet No virus was 480 SSI 118 Pox panel 2019 Pox panel Orthopox Not analysed yet! identified (13#) 2019 (13#) previously surveillance No virus was samples from identified 481 SSI 119 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 482 SSI 120 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 483 SSI 121 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 484 SSI 122 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 485 SSI 123 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 486 SSI 124 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 487 SSI 125 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 488 SSI 126 blood Unknown Negative migrating birds previously (2018)

surveillance No virus was samples from identified 489 SSI 127 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 490 SSI 128 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 491 SSI 129 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 492 SSI 130 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 493 SSI 131 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 494 SSI 132 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 495 SSI 133 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 496 SSI 134 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 497 SSI 135 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 498 SSI 136 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 499 SSI 137 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 500 SSI 138 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 501 SSI 139 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 502 SSI 140 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 503 SSI 141 blood Unknown Negative migrating birds previously (2018) surveillance No virus was samples from identified 504 SSI 142 blood Unknown Not analysed yet! migrating birds previously (2018) surveillance No virus was samples from 505 SSI 143 blood Unknown Not analysed yet! identified migrating birds previously (2018)

surveillance No virus was samples from 506 SSI 144 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 507 SSI 145 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 508 SSI 146 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 509 SSI 147 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 510 SSI 148 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 511 SSI 149 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 512 SSI 150 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 513 SSI 151 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 514 SSI 152 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 515 SSI 153 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 516 SSI 154 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 517 SSI 155 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 518 SSI 156 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 519 SSI 157 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 520 SSI 158 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 521 SSI 159 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 522 SSI 160 blood Unknown Not analysed yet! identified migrating birds previously (2018)

surveillance No virus was samples from 523 SSI 161 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 524 SSI 162 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 525 SSI 163 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 526 SSI 164 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 527 SSI 165 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 528 SSI 166 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 529 SSI 167 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 530 SSI 168 blood Unknown Not analysed yet! identified migrating birds previously (2018) surveillance No virus was samples from 531 SSI 169 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 532 SSI 170 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 533 SSI 171 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 534 SSI 172 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 535 SSI 173 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 536 SSI 174 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 537 SSI 175 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 538 SSI 176 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 539 SSI 177 blood Unknown Not analysed yet! identified migrating birds previously (2017)

surveillance No virus was samples from 540 SSI 178 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 541 SSI 179 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 542 SSI 180 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 543 SSI 181 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 544 SSI 182 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 545 SSI 183 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 546 SSI 184 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 547 SSI 185 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 548 SSI 186 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 549 SSI 187 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 550 SSI 188 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 551 SSI 189 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 552 SSI 190 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 553 SSI 191 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 554 SSI 192 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 555 SSI 193 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 556 SSI 194 blood Unknown Not analysed yet! identified migrating birds previously (2017)

surveillance No virus was samples from 557 SSI 195 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 558 SSI 196 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 559 SSI 197 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 560 SSI 198 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 561 SSI 199 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 562 SSI 200 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 563 SSI 201 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 564 SSI 202 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 565 SSI 203 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 566 SSI 204 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 567 SSI 205 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 568 SSI 206 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 569 SSI 207 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 570 SSI 208 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 571 SSI 209 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 572 SSI 210 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 573 SSI 211 blood Unknown Not analysed yet! identified migrating birds previously (2017)

surveillance No virus was samples from 574 SSI 212 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 575 SSI 213 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 576 SSI 214 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 577 SSI 215 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 578 SSI 216 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 579 SSI 217 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 580 SSI 218 blood Unknown Not analysed yet! identified migrating birds previously (2017) surveillance No virus was samples from 581 SSI 219 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 582 SSI 220 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 583 SSI 221 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 584 SSI 222 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 585 SSI 223 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 586 SSI 224 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 587 SSI 225 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 588 SSI 226 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 589 SSI 227 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 590 SSI 228 blood Unknown Not analysed yet! identified migrating birds previously (2016)

surveillance No virus was samples from 591 SSI 229 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 592 SSI 230 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 593 SSI 231 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 594 SSI 232 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 595 SSI 233 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 596 SSI 234 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 597 SSI 235 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 598 SSI 236 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 599 SSI 237 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 600 SSI 238 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 601 SSI 239 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 602 SSI 240 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 603 SSI 241 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 604 SSI 242 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 605 SSI 243 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 606 SSI 244 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 607 SSI 245 blood Unknown Not analysed yet! identified migrating birds previously (2016)

surveillance No virus was samples from 608 SSI 246 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 609 SSI 247 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 610 SSI 248 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 611 SSI 249 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 612 SSI 250 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 613 SSI 251 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 614 SSI 252 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 615 SSI 253 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 616 SSI 254 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 617 SSI 255 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 618 SSI 256 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 619 SSI 257 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 620 SSI 258 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 621 SSI 259 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 622 SSI 260 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 623 SSI 261 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 624 SSI 262 blood Unknown Not analysed yet! identified migrating birds previously (2016)

surveillance No virus was samples from 625 SSI 263 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 626 SSI 264 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 627 SSI 265 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 628 SSI 266 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 629 SSI 267 blood Unknown Not analysed yet! identified migrating birds previously (2016) surveillance No virus was samples from 630 SSI 268 blood Unknown Not analysed yet! identified migrating birds previously (2016)