Supplementary Table 1: Summary of information for all falling within the seven genera in our analyses.

Accession Genome Genus Species name Strain Abbreviation Source Country Reference Nucleotide Amino acid Size (nt) #ORFs Marnavirus Heterosigma akashiwo RNA Heterosigma Lang et al. , 2004; HaRNAV AY337486 AAP97137 8587 One Canada RNA virus 1 virus akashiwo Tai et al. , 2003

Marine single- ASG92540 Moniruzzaman et Classification pending Q sR OV 020 KY286100 9290 Two celled USA ASG92541 al ., 2017 Marine single- Moniruzzaman et Classification pending Q sR OV 041 KY286101 ASG92542 9328 One celled USA al ., 2017 eukaryotes APG78557 Classification pending Wenzhou picorna-like virus 13 WZSBei69459 KX884360 9458 One Bivalve China Shi et al ., 2016 APG78557

Classification pending Changjiang picorna-like virus 2 CJLX30436 KX884547 APG79001 7171 One Crayfish China Shi et al ., 2016

Beihai picorna-like virus 57 BHHQ57630 KX883356 APG76773 8518 One Tunicate China Shi et al ., 2016 Classification pending Beihai picorna-like virus 57 BHJP51916 KX883380 APG76812 8518 One Tunicate China Shi et al ., 2016 Marine single- ASG92530 Moniruzzaman et Classification pending N OV 137 KY130494 7746 Two celled USA ASG92531 al ., 2017 eukaryotes Hubei picorna-like virus 7 WHSF7327 KX884284 APG78434 9614 One Pill worm China Shi et al ., 2016 Classification pending Hubei picorna-like virus 7 WHCC111241 KX884268 APG78407 7945 One Insect China Shi et al ., 2016 Sanxia atyid shrimp virus 2 WHCCII13331 KX884278 APG78424 10445 One Insect China Shi et al ., 2016 Classification pending Freshwater atyid Sanxia atyid shrimp virus 2 SXXX37884 KX883708 APG77465 10400 One China Shi et al ., 2016 shrimp Aurantiochytrium single Aurantiochytrium single stranded BAE47143 Aurantiochytriu AuRNAV AB193726 9035 Three4 Japan Takao et al. , 2006 stranded RNA virus 1 RNA virus YP_398835 m sp. López-Bueno et Classification pending Antarctic picorna-like virus 3 APLV3 KM259871 AKG93964 8644 One Lake Antarctica al ., 2015 APG77377 Freshwater Shahe picorna-like virus 6 SHWC0209c11084 KX883653 8831 Two China Shi et al ., 2016 APG77378 heteroptera Classification pending APG77381 Freshwater Shahe picorna-like virus 6 SHWC13532 KX883655 8831 Two China Shi et al ., 2016 APG77382 arthropod APG78591 Classification pending Wenzhou picorna-like virus 19 WZSLuoII97616 KX884381 10153 Two Gastropod China Shi et al ., 2016 APG78592 Classification pending Beihai picorna-like virus 56 BWBFG40791 KX884529 APG78971 10546 One Paphia shell China Shi et al ., 2016 Beihai picorna-like virus 50 BHBei76574 KX883311 APG76693 7543 One Bivalve China Shi et al ., 2016 Classification pending Beihai picorna-like virus 50 BHZC37234 KX884484 APG78898 7599 One Razor shell China Shi et al ., 2016 Channeled Classification pending Wenzhou picorna-like virus 22 WZFSL83961 KX884331 APG78507 8890 One China Shi et al ., 2016 applesnail Channeled Classification pending Wenzhou picorna-like virus 23 WZFSL85852 KX884324 APG78497 9223 One China Shi et al ., 2016 applesnail Beihai picorna-like virus 59 BHBei77099 KX883321 APG76708 9096 One Bivalve China Shi et al ., 2016 Classification pending Beihai picorna-like virus 59 BHZC37451 KX884500 APG78921 9096 One Razor shell China Shi et al ., 2016 APG76888 Classification pending Beihai picorna-like virus 63 BHTSS17878 KX883419 8334 Two Woodlouse China Shi et al ., 2016 APG76889 APG76771 Beihai picorna-like virus 64 BHHQ137467 KX883355 8646 Two Tunicate China Shi et al ., 2016 APG76889 Classification pending APG76815 Beihai picorna-like virus 64 BHJP54755 KX883382 8647 Two Echinoderm China Shi et al ., 2016 APG76816 Labyrnavirus

Classification pending Beihai picorna-like virus 62 BHBei74566 KX883309 APG76690 8507 One Bivalve China Shi et al ., 2016 Classification pending Beihai picorna-like virus 61 BHBei76813 KX883313 APG76696 9113 One Bivalve China Shi et al ., 2016 Classification pending Beihai picorna-like virus 60 BHBei55726 KX883305 APG76685 8818 One Bivalve China Shi et al ., 2016 APG78560 Classification pending Wenzhou picorna-like virus 24 WZSBei69493 KX884362 8778 Two Bivalve China Shi et al ., 2016 APG78560 Locarnavirus ABQ50601 Jericarnavirus B 2 Marine RNA virus JP-B JP-B EF198242 8926 Two Coastal marine Canada Culley et al. , 2007 ABQ50602 AGZ83339 Coastal Greninger and Sanfarnavirus 2 Marine RNA virus SF-2 SF-2 KF412901 9321 Two USA AGZ83340 wastewater DeRisi, 2015 AFM44930 Coastal Greninger and Sanfarnavirus 1 Marine RNA virus SF-1 SF-1 JN661160 8970 Two USA AFM44929 wastewater DeRisi, 2015 Coastal Greninger and Sanfarnavirus 3 Marine RNA virus SF-3 SF-3 KF478836 AHA44480 8648 One USA wastewater DeRisi, 2015 APG78020 Classification pending Beihai picorna-like virus 42 SCJXSX39263 KX883963 9166 Two Sesarmid crab China Shi et al ., 2016 APG78021 APG76706 Classification pending Beihai picorna-like virus 58 BHBei77093 KX883320 8411 Two Bivalve China Shi et al ., 2016 APG76707 Freshwater atyid Classification pending Sanxia picorna-like virus 8 SXXX35540 KX883720 APG77485 7418 One China Shi et al ., 2016 shrimp

Classification pending Changjiang picorna-like virus 1 CJLX3014 KX884546 APG79000 8729 One Crayfish China Shi et al ., 2016

APG78521 Channeled Classification pending Wenzhou picorna-like virus 12 WZFSL74240 KX884340 9025 Two China Shi et al ., 2016 APG78522 applesnail Marine single- ASG92524 Moniruzzaman et Classification pending N OV 080 KY130491 8584 Two celled USA ASG92525 al ., 2017 eukaryotes APG78593 Classification pending Wenzhou picorna-like virus 14 WZSLuoII97619 KX884382 8951 Two Gastropod China Shi et al ., 2016 APG78594 APG76734 Beihai picorna-like virus 43 BHBJDX18749 KX883335 8935 Two Penaeid shrimp China Shi et al ., 2016 APG76735 APG76791 Beihai picorna-like virus 43 BHJJX25682 KX883367 8328 Two Hermit crab China Shi et al ., 2016 APG76792 APG76908 Classification pending Beihai picorna-like virus 43 BHWZXX15634 KX883429 8935 Two Penaeid shrimp China Shi et al ., 2016 APG76909 APG76831 Beihai picorna-like virus 43 BHNXC41212 KX883390 8943 Two Peanut worm China Shi et al ., 2016 APG76832 APG78018 Beihai picorna-like virus 43 SCJXSX39249 KX883962 8522 Two Sesarmid crab China Shi et al ., 2016 APG78019 APG78992 Classification pending Changjiang picorna-like virus 3 CJLX28786 KX884542 9316 Two Crayfish China Shi et al ., 2016 APG78993 APG78502 Channeled Classification pending Wenzhou picorna-like virus 15 WZFSL85504 KX884328 8610 Two China Shi et al ., 2016 APG78503 applesnail APG76697 Classification pending Beihai razor shell virus 2 BHBei76898 KX883314 8702 Two Bivalve China Shi et al ., 2016 APG76698 APG78903 Classification pending Beihai razor shell virus 2 BHZC37307 KX884488 8430 Two Razor shell China Shi et al ., 2016 APG78904 APG78900 Beihai razor shell virus 1 BHZC37272 KX884486 8628 Two Razor shell China Shi et al ., 2016 APG78901 APG76694 Classification pending Beihai razor shell virus 1 BHBei76693 KX883312 8349 Two Bivalve China Shi et al ., 2016 APG76695 APG76730 Beihai razor shell virus 1 BHBJDX18719 KX883333 8627 Two Penaeid shrimp China Shi et al ., 2016 APG76731 Locarnavirus

APG78866 Classification pending Beihai picorna-like virus 44 BHXG26143 KX884466 8711 Two Mantis shrimp China Shi et al ., 2016 APG78867 Freshwater Shahe picorna-like virus 4 SHWC0209c12741 KX883645 APG77365 8853 One China Shi et al ., 2016 isoptera Classification pending Freshwater Shahe picorna-like virus 4 SHWC13603 KX883659 APG77387 8860 One China Shi et al ., 2016 arthropod APG78911 Classification pending Beihai picorna-like virus 46 BHZC37388 KX884493 9153 Two Razor shell China Shi et al ., 2016 APG78912 Classification pending Beihai picorna-like virus 45 BHZC37433 KX884496 APG78916 6098 One Razor shell China Shi et al ., 2016 APG77498 Classification pending Hubei leech virus 3 SZmix63518 KX883727 8848 Two Leech China Shi et al ., 2016 APG77499 APG78398 Classification pending Hubei picorna-like virus 2 WHBL73152 KX884264 9147 Two Myriapod China Shi et al ., 2016 APG78399 APG78402 Classification pending Hubei picorna-like virus 3 WHCC101453 KX884266 8824 Two Insect China Shi et al ., 2016 APG78403 Channeled Classification pending Wenzhou picorna-like virus 16 WZFSL67369 KX884342 APG78525 6372 One China Shi et al ., 2016 applesnail APG78024 Classification pending Beihai picorna-like virus 47 SCJXSX39359 KX883965 8920 Two Sesarmid crab China Shi et al ., 2016 APG78025 APG77478 Freshwater atyid Classification pending Sanxia picorna-like virus 9 SXXX36560 KX883717 8114 Two China Shi et al ., 2016 APG77479 shrimp APG78918 Classification pending Beihai picorna-like virus 48 BHZC37443 KX884498 9382 Two Razor shell China Shi et al ., 2016 APG78918 APG77383 Freshwater Classification pending Shahe picorna-like virus 5 SHWC13542 KX883656 8658 Two China Shi et al ., 2016 APG77384 arthropod Wenzhou channeled applesnail APG78504 Channeled Classification pending WZFSL85493 KX884329 8943 Two China Shi et al ., 2016 virus 1 APG78505 applesnail APG78400 Freshwater Classification pending Hubei picorna-like virus 4 WHBL90007 KX884265 8604 Two China Shi et al ., 2016 APG78401 shellfish APG78392 Freshwater Classification pending Hubei picorna-like virus 5 WHBL70549 KX884261 8763 Two China Shi et al ., 2016 APG78393 shellfish APG78994 Classification pending Changjiang picorna-like virus 4 CJLX29654 KX884543 8923 Two Crayfish mix China Shi et al ., 2016 APG78995 APG78891 Classification pending Beihai picorna-like virus 49 BHZC36855 KX884480 8407 Two Razor shell China Shi et al ., 2016 APG78892 APG77494 Hubei leech virus 4 SZmix20870 KX883725 8267 Two Leech China Shi et al ., 2016 APG77495 Classification pending APG79016 Hubei leech virus 4 CJLX30730 KX884556 8074 Two Crayfish China Shi et al ., 2016 APG79017 Kusarnavirus Asterionellopsis glacialis RNA BAP16719 Asterionellopsis Tomaru et al. , Astarnavirus 2 AglaRNAV AB973945 8842 Two Japan virus BAP16720 glacialis 2012 AMB17481 Lachnit et al ., Classification pending Delisea pulchra RNA virus KT455464 9581 Two Delisea pulchra Australia AMB17482 2016 APG76861 Beihai sipunculid worm virus 3 BHNXC41487 KX883406 6134 Two Peanut worm China Shi et al ., 2016 APG76862 Classification pending APG78922 Beihai sipunculid worm virus 3 BHZC37455 KX884501 9110 Two Razor shell China Shi et al ., 2016 APG78923 APG76858 Classification pending Beihai sipunculid worm virus 2 BHNXC41483 KX883404 9395 Two Peanut worm China Shi et al ., 2016 APG76859 APG78919 Classification pending Beihai picorna-like virus 31 BHZC37445 KX884499 7425 Two Razor shell China Shi et al ., 2016 APG78920 Kusarnavirus

APG78555 Classification pending Wenzhou picorna-like virus 9 WZSBei69430 KX884359 9171 Two Bivalve China Shi et al ., 2016 APG78556 APG78585 Beihai Mollusks virus 1 WZSLuoI86140 KX884377 8805 Two Gastropod China Shi et al ., 2016 APG78586 APG76840 Beihai Mollusks virus 1 BHNXC41338 KX883395 7373 Two Peanut worm China Shi et al ., 2016 APG76841 APG78876 Beihai Mollusks virus 1 BHXG26682 KX884472 9042 Two Mantis shrimp China Shi et al ., 2016 APG78877 APG76764 Classification pending Beihai Mollusks virus 1 BHHK131425 KX883351 9046 Two Sea anemones China Shi et al ., 2016 APG76765 APG78610 Blue swimmer Beihai Mollusks virus 1 YYSZX17807 KX884391 9004 Two China Shi et al ., 2016 APG78611 crab APG78936 Beihai Mollusks virus 1 BHZY60617 KX884509 9004 Two Octopus China Shi et al ., 2016 APG78937 APG78969 Beihai Mollusks virus 1 BWBFG40783 KX884528 9034 Two Paphia shell China Shi et al ., 2016 APG78970 APG78972 Classification pending Beihai picorna-like virus 33 BWBFG40845 KX884530 8887 Two Paphia shell China Shi et al ., 2016 APG78973 APG78952 Beihai mollusks virus 2 BHZY60889 KX884518 8402 Two Octopus China Shi et al ., 2016 APG78953 APG78581 Classification pending Beihai mollusks virus 2 WZSLuoI86113 KX884375 9054 Two Gastropoda China Shi et al ., 2016 APG78582 APG78924 Beihai mollusks virus 2 BHZC37403 KX884502 8017 Two Razor shell China Shi et al ., 2016 APG78925 APG76842 Beihai sipunculid worm virus 4 BHNXC41347 KX883396 8486 Two Peanut worm China Shi et al ., 2016 APG76843 APG76714 Classification pending Beihai sipunculid worm virus 4 BHBJDX17813 KX883325 8288 Two Penaeid shrimp China Shi et al ., 2016 APG76715 APG78907 Beihai sipunculid worm virus 4 BHZC37368 KX884491 8487 Two Razor shell China Shi et al ., 2016 APG78908 Beihai picorna-like virus 32 BHNXC41402 KX883397 APG76844 6040 One Peanut worm China Shi et al ., 2016 Classification pending Beihai picorna-like virus 32 BHZC37269 KX884485 APG78899 6036 One Razor shell China Shi et al ., 2016 APG77928 Classification pending Beihai picorna-like virus 34 HOU158088 KX883902 5391 Two Horseshoe crab China Shi et al ., 2016 APG77929 APG78531 Wenzhou picorna-like virus 10 WZRBX43164 KX884346 9142 Two Charybdis crab China Shi et al ., 2016 APG78532 Classification pending APG78583 Wenzhou picorna-like virus 10 WZSLuoI86114 KX884376 8912 Two Gastropoda China Shi et al ., 2016 APG78584 APG78896 Beihai picorna-like virus 35 BHZC37213 KX884483 8955 Two Razor shell China Shi et al ., 2016 APG78897 APG78604 Blue swimmer Beihai picorna-like virus 35 YYSZX17573 KX884388 8905 Two China Shi et al ., 2016 APG78605 crab APG77936 Beihai picorna-like virus 35 HWRTX12817 KX883907 8937 Two Tiger crab China Shi et al ., 2016 APG77937 APG78014 Beihai picorna-like virus 35 SCJXSX39018 KX883960 8918 Two Sesarmid crab China Shi et al ., 2016 APG78015 APG76890 Beihai picorna-like virus 35 BHTSS17888 KX883420 8912 Two Woodlouse China Shi et al ., 2016 APG76891 APG76899 Beihai picorna-like virus 35 BHWZXX15273 KX883425 8942 Two Penaeid shrimp China Shi et al ., 2016 APG76900 APG76877 Classification pending Beihai picorna-like virus 35 BHTH15623 KX883412 8797 Two Barnacle China Shi et al ., 2016 APG76878 Kusarnavirus

Classification pending

APG76823 Beihai picorna-like virus 35 BHNXC40527 KX883386 8915 Two Peanut worm China Shi et al ., 2016 APG76824 APG76752 Beihai picorna-like virus 35 BHHK128006 KX883345 8937 Two Sea anemone China Shi et al ., 2016 APG76753 APG76710 Beihai picorna-like virus 35 BHBJDX17224 KX883323 8945 Two Penaeid shrimp China Shi et al ., 2016 APG76711 APG78978 Turritella sea Beihai picorna-like virus 35 BZL82652 KX884534 8939 Two China Shi et al ., 2016 APG78979 snail APG78868 Beihai picorna-like virus 35 BHXG26244 KX884467 8890 Two Mantis shrimp China Shi et al ., 2016 APG78869 APG76783 Beihai picorna-like virus 35 BHJJX23572 KX883363 8937 Two Hermit crab China Shi et al ., 2016 APG76784 Chaetoceros tenuissimus Chaetoceros tenuissimus RNA BAG30951 Chaetoceros CtenRNAV01 AB375474 9431 Two Japan Shirai et al. , 2008 RNA virus 01 virus 01 BAG30952 tenuissimus

Rhizosolenia setigera Rhizosolenia setigera RNA BAE79742 Rhizosolenia Nagasaki et al. , RsRNAV AB243297 8877 Two Japan RNA virus 1 virus1 BAE79743 setigera 2004 Chaetoceros Chaetoceros socialis f. Chaetoceros socialis f. radians BAH22517 Tomaru et al. , CsfrRNAV AB469874 9467 Two socialis f. Japan radians RNA virus 01 RNA virus 01 BAH22518 2009 radians Marine single- ASG92534 Moniruzzaman et Classification pending N OV 003 KY130496 7005 Two celled USA ASG92535 al ., 2017 eukaryotes APG76780 Classification pending Beihai picorna-like virus 25 BHJJX20433 KX883361 7513 Two Hermit crab China Shi et al ., 2016 APG76781 APG78933 Classification pending Beihai picorna-like virus 24 BHZY60459 KX884507 9665 Two Octopus China Shi et al ., 2016 APG78934 APG78982 Turritella sea Classification pending Beihai picorna-like virus 26 BZL93356 KX884536 9864 Two China Shi et al ., 2016 APG78983 snail APG76847 Classification pending Beihai picorna-like virus 27 BHNXC41439 KX883399 9410 Two Peanut worm China Shi et al ., 2016 APG76848 APG78940 Classification pending Beihai picorna-like virus 28 BHZY60695 KX884511 9559 Two Octopus China Shi et al ., 2016 APG78941 Salisharnavirus AYD68779 Coastal/oceanic Britarnavirus 4 2 Marine RNA virus BC-4 BC-4 MH171300 8593 Two Canada Unpublished3 AYD68780 marine AMK49159 Miranda et al. , Palmarnavirus 473 Marine RNA virus PAL473 PAL473 KT727026 6360 Two Coastal marine USA AMK49160 2016 AYD68773 Britarnavirus 1 Marine RNA virus BC-1 BC-1 MG584187 8638 Two Coastal marine Canada Vlok et al. , 2019 AYD68774 AMK49153 Miranda et al. , Palmarnavirus 128 Marine RNA virus PAL128 PAL128 KT727023 8660 Two Coastal marine USA AMK49154 2016 APG77486 Freshwater atyid Classification pending Sanxia picorna-like virus 5 SXXX34146 KX883721 8673 Two China Shi et al ., 2016 APG77487 shrimp APG77469 Freshwater atyid Classification pending Sanxia picorna-like virus 1 SXXX37713 KX883711 9301 Two China Shi et al ., 2016 APG77470 shrimp APG77466 Freshwater atyid Classification pending Sanxia picorna-like virus 2 SXXX37883 KX883709 9058 Two China Shi et al ., 2016 APG77467 shrimp APG77397 Freshwater Classification pending Shahe picorna-like virus 1 SHWC13622 KX883666 9822 Two China Shi et al ., 2016 APG77398 arthropod Freshwater atyid Classification pending Sanxia picorna-like virus 4 SXXX37816 KX883710 APG77468 7388 One China Shi et al ., 2016 shrimp Salisharnavirus

APG77480 Freshwater atyid Classification pending Sanxia picorna-like virus 3 SXXX36208 KX883718 8903 Two China Shi et al ., 2016 APG77481 shrimp

Classification pending Wenzhou picorna-like virus 18 shrimp14534 KX883983 APG78057 6682 One Shrimp China Shi et al ., 2016

Marine single- ASG92522 Moniruzzaman et Classification pending N OV 011 KY130490 9068 Two celled USA ASG92523 al ., 2017 eukaryotes APG78574 Classification pending Wenzhou picorna-like virus 4 WZSLuoI86003 KX884371 9345 Two Gastropoda China Shi et al ., 2016 APG78575 APG76869 Classification pending Beihai picorna-like virus 18 BHSC157282 KX883409 9186 Two Sandworm China Shi et al ., 2016 APG76870 APG78028 Classification pending Beihai sesarmid crab virus 1 SCJXSX39422 KX883967 8870 Two Sesarmid crab China Shi et al ., 2016 APG78029 APG77496 Classification pending Hubei leech virus 2 SZmix21584 KX883726 9073 Two Leech China Shi et al ., 2016 APG77497 APG79027 Classification pending Changjiang crawfish virus 1 CJLX30787 KX884562 9103 Two Crayfish China Shi et al ., 2016 APG79028 APG78929 Classification pending Beihai picorna-like virus 17 BHZY55665 KX884505 8733 Two Octopus China Shi et al ., 2016 APG78930 APG76785 Classification pending Beihai picorna-like virus 19 BHJJX24523 KX883364 8839 Two Hermit crab China Shi et al ., 2016 APG76786 APG76827 Classification pending Beihai sipunculid worm virus 1 BHNXC40689 KX883388 8879 Two Peanut worm China Shi et al ., 2016 APG76828 Freshwater atyid Classification pending Sanxia picorna-like virus 10 SXXX37528 KX883715 APG77476 9596 One China Shi et al ., 2016 shrimp Marine single- Moniruzzaman et Classification pending Q sR OV 022 KY286105 ASG92549 8561 One celled USA al ., 2017 eukaryotes APG78980 Turritella sea Beihai Picorna-like virus 2 BZL93063 KX884535 9980 Two China Shi et al ., 2016 APG78981 snail APG77940 Beihai Picorna-like virus 2 HWRTX12828 KX883909 9969 Two Tiger crab China Shi et al ., 2016 APG77941 APG76906 Beihai Picorna-like virus 2 BHWZXX15591 KX883428 9976 Two Penaeid shrimp China Shi et al ., 2016 APG76907 APG78944 Beihai Picorna-like virus 2 BHZY60755 KX884513 9980 Two Octopus China Shi et al ., 2016 APG78945 Classification pending APG76825 Beihai Picorna-like virus 2 BHNXC40556 KX883387 9983 Two Peanut worm China Shi et al ., 2016 APG76826 APG76795 Beihai Picorna-like virus 2 BHJJX25716 KX883369 9981 Two Hermit crab China Shi et al ., 2016 APG76796 APG76716 Beihai Picorna-like virus 2 BHBJDX18281 KX883326 9981 Two Penaeid shrimp China Shi et al ., 2016 APG76717 APG76756 Beihai Picorna-like virus 2 BHHK129932 KX883347 9975 Two Sea anemone China Shi et al ., 2016 APG76757 Marine single- Marine single- ASG92520 Moniruzzaman et Classification pending N OV 039 KY130489 9264 Two celled celled ASG92521 al ., 2017 eukaryotes eukaryotes AKG93965 López-Bueno et Classification pending Antarctic picorna-like virus 4 APLV4 KM259872 9168 Two Lake Antarctica AKG93966 al ., 2015 APG77366 Freshwater Classification pending Shahe picorna-like virus 2 SHWC0209c12507 KX883646 9272 Two China Shi et al ., 2016 APG77367 isoptera Salisharnavirus

APG78948 Classification pending Beihai picorna-like virus 21 BHZY60822 KX884515 8817 Two Octopus China Shi et al ., 2016 APG78949 APG78558 Classification pending Wenzhou picorna-like virus 32 WZSBei69487 KX884361 9319 Two Bivalve China Shi et al ., 2016 APG78559

Classification pending Beihai picorna-like virus 22 HOU126951 KX883905 APG77933 6975 Two Horseshoe crab China Shi et al ., 2016

APG78926 Classification pending Beihai octopus virus 1 BHZY180716 KX884503 9211 Two Octopus China Shi et al ., 2016 APG78927 APG78055 Classification pending Wenzhou picorna-like virus 7 shrimp14504 KX883982 8783 Two Shrimp China Shi et al ., 2016 APG78056 APG78565 Classification pending Wenzhou picorna-like virus 6 WZSBei69574 KX884366 9549 Two Bivalve China Shi et al ., 2016 APG78566 Marine single- ASG92547 Moniruzzaman et Classification pending Q sR OV 019 KY286104 8921 Two celled USA ASG92548 al ., 2017 eukaryotes APG78946 Beihai picorna-like virus 23 BHZY60791 KX884514 9127 Two Octopus China Shi et al ., 2016 APG78947 APG78600 Blue swimmer Classification pending Beihai picorna-like virus 23 YYSZX17154 KX884386 9176 Two China Shi et al ., 2016 APG78601 crab APG78965 Beihai picorna-like virus 23 BWBFG39274 KX884526 9069 Two Paphia shell China Shi et al ., 2016 APG78966 Sogarnavirus AYD68775 Britarnavirus 2 Marine RNA virus BC-2 BC-2 MG584188 8843 Two Coastal marine Canada Vlok et al. , 2019 AYD68776 AMK49155 Miranda et al. , Palmarnavirus 156 Marine RNA virus PAL156 PAL156 KT727024 7897 Two Coastal marine USA AMK49156 2016 AYD68777 Britarnavirus 3 Marine RNA virus BC-3 BC-3 MG584189 8496 Two Coastal marine Canada Vlok et al. , 2019 AYD68778 ABQ50599 Jericarnavirus A Marine RNA virus JP-A JP-A EF198241 9236 Two Coastal marine Canada Culley et al. , 2007 ABQ50600 Chaetoceros tenuissimus RNA BAP99818 Chaetoceros Kimura and Chaetenuissarnavirus II 2 CtenRNAVII AB971661 9562 Two Japan virus type II2 BAP99819 tenuissimus Tomaru, 2015 Chaetoceros species RNA virus BAK40203 Tomaru et al. , Chaetarnavirus 2 CspRNAV2 AB639040 9417 Two Chaetoceros sp Japan 02 BAK40204 2013 APG78938 Classification pending Beihai picorna-like virus 4 BHZY60671 KX884510 9376 Two Octopus China Shi et al ., 2016 APG78939 APG76675 Wenzhou picorna-like virus 2 beimix73672 KX883299 8895 Two Bivalve China Shi et al ., 2016 APG76676 Classification pending APG78545 Wenzhou picorna-like virus 2 WZSBei58345 KX884353 8895 Two Bivalve China Shi et al ., 2016 APG78546 APG76845 Classification pending Beihai picorna-like virus 5 BHNXC41415 KX883398 9888 Two Peanut worm China Shi et al ., 2016 APG76846 APG76855 Classification pending Beihai picorna-like virus 7 BHNXC41478 KX883403 9338 Two Peanut worm China Shi et al ., 2016 APG76857 APG76837 Beihai picorna-like virus 6 BHNXC41284 KX883393 9416 Two Peanut worm China Shi et al ., 2016 APG76838 Classification pending APG78909 Beihai picorna-like virus 6 BHZC37373 KX884492 9370 Two Razor shell China Shi et al ., 2016 APG78910 APG76736 Classification pending Beihai picorna-like virus 9 BHBJDX18814 KX883336 8677 Two Penaeid shrimp China Shi et al ., 2016 APG76737 APG76851 Classification pending Beihai picorna-like virus 8 BHNXC41454 KX883401 9273 Two Peanut worm China Shi et al ., 2016 APG76852 Sogarnavirus

APG78954 Classification pending Beihai picorna-like virus 11 BHZY60905 KX884519 9245 Two Octopus China Shi et al ., 2016 APG78955 APG78572 Classification pending Wenzhou picorna-like virus 1 WZSLuoI85940 KX884370 8804 Two Gastropod China Shi et al ., 2016 APG78573 APG76677 Classification pending Wenzhou picorna-like virus 52 beimix75583 KX883300 8991 Two Bivalve China Shi et al ., 2016 APG76678 AKG93962 López-Bueno et Classification pending Antarctic picorna-like virus 2 APLV2 KM259870 9357 Two Lake Antarctica AKG93963 al ., 2015 APG78567 Classification pending Wenzhou picorna-like virus 50 WZSLuoI83448 KX884367 8456 Two Gastropod China Shi et al ., 2016 APG78568 APG76892 Beihai picorna-like virus 1 BHTSS18000 KX883421 9139 Two Woodlouse China Shi et al ., 2016 APG76893 APG76835 Beihai picorna-like virus 1 BHNXC41230 KX883392 9144 Two Peanut worm China Shi et al ., 2016 APG76836 APG76728 Beihai picorna-like virus 1 BHBJDX18697 KX883332 9148 Two Penaeid shrimp China Shi et al ., 2016 APG76729 Classification pending APG78893 Beihai picorna-like virus 1 BHZC36988 KX884481 9149 Two Razor shell China Shi et al ., 2016 APG78894 APG78022 Beihai picorna-like virus 1 SCJXSX39332 KX883964 9148 Two Sesarmid crab China Shi et al ., 2016 APG78023 APG76799 Beihai picorna-like virus 1 BHJJX25883 KX883371 9147 Two Hermit crab China Shi et al ., 2016 APG76800 Classification pending Beihai picorna-like virus 13 BHZC37435 KX884497 APG78917 6192 One Razor shell China Shi et al ., 2016 APG76758 Classification pending Beihai picorna-like virus 14 BHHK130444 KX883348 9234 Two Sea anemone China Shi et al ., 2016 APG76759 APG78976 Turritella sea Beihai picorna-like virus 15 BZL81977 KX884533 9088 Two China Shi et al ., 2016 APG78977 snail APG78931 Beihai picorna-like virus 15 BHZY59344 KX884506 9110 Two Octopus China Shi et al ., 2016 APG78932 APG78880 Beihai picorna-like virus 15 BHXun32793 KX884474 9074 Two Charybdis crab China Shi et al ., 2016 APG78881 APG78889 Beihai picorna-like virus 15 BHZC36638 KX884479 9090 Two Razor shell China Shi et al ., 2016 APG78890 APG78862 Beihai picorna-like virus 15 BHXG24735 KX884464 9107 Two Mantis shrimp China Shi et al ., 2016 APG78863 APG78602 Blue swimmer Beihai picorna-like virus 15 YYSZX17489 KX884387 9088 Two China Shi et al ., 2016 APG78603 crab APG77938 Beihai picorna-like virus 15 HWRTX12823 KX883908 9082 Two Tiger crab China Shi et al ., 2016 APG77939 Classification pending APG76886 Beihai picorna-like virus 15 BHTSS17870 KX883418 9070 Two Woodlouse China Shi et al ., 2016 APG76887 APG76912 Beihai picorna-like virus 15 BHWZXX15642 KX883431 9065 Two Penaeid shrimp China Shi et al ., 2016 APG76913 APG76833 Beihai picorna-like virus 15 BHNXC41227 KX883391 9092 Two Peanut worm China Shi et al ., 2016 APG76834 APG76797 Beihai picorna-like virus 15 BHJJX25823 KX883370 9083 Two Hermit crab China Shi et al ., 2016 APG76798 APG76724 Beihai picorna-like virus 15 BHBJDX18577 KX883330 9074 Two Penaeid shrimp China Shi et al ., 2016 APG76725 APG78961 Beihai picorna-like virus 15 BWBFG37850 KX884524 9076 Two Paphia shell China Shi et al ., 2016 APG78962 Sogarnavirus

Classification pending

APG76741 Beihai picorna-like virus 15 BHCL77416 KX883339 9109 Two Murex snail China Shi et al ., 2017 APG76742 1 Assigned type species in existing genera. 2 Type species in new genera. 3 Genome assembly described in Vlok et al. unpublished. 4 AuRNAV has a third small overlapping open reading frame that produces a sub-genomic RNA. While we cannot exclude the existence of similar ORFs in other genomes, none has been experimentally demonstrated to encode protein. Grey cells indicate viruses that will require special consideration for species classification as indicated in Supplementary Table 4. Supplementary Table 2: GenBank accession numbers for additional sequences used in this study.

GenBank Accession Family Genus Nucleotide Amino Acid Species or virus name Genome RdRp Capsid ABO41208 ABO41208 Triticum mosaic virus Potyviridae AF057533 AAC13692 Wheat streak mosaic virus Potyviridae Z73124 CAA97466 Sweet potato mild mottle virus Potyviridae AJ132268 CAA10637 Barley yellow mosaic virus Potyviridae AY994084 AAX87001 Blackberry virus Y Potyviridae X12456 CAA30988 Potato virus Y Potyviridae Y09854 CAA70983 Ryegrass mosaic virus Picornaviridae Ampivirus KP770140 AKJ23343 Ampivirus A Picornaviridae Kunsagivirus KC935379 AGO59796 Kunsagivirus A Picornaviridae EU142040 ABV57376 Aquamavirus A Picornaviridae DQ226541 ABB22725 Avihepatovirus A Picornaviridae KC614703 AGH06056 Avisivirus A Picornaviridae Potamipivirus KC843627 AGV08598 Potamipivirus A Picornaviridae JQ316470 AFN70966 Pasivirus A Picornaviridae Limnipivirus JX134222 AFP96991 Limnipivirus A Picornaviridae S45208 AAB23363 Parechovirus A Picornaviridae Hepatovirus M14707 AAA45465 Hepatovirus A Picornaviridae Tremovirus AJ225173 CAA12416 Tremovirus A Picornaviridae Harkavirus KP230449 AKC42744 Harkavirus A Picornaviridae Rabovirus KP233897 AJF23050 Rabovirus A Picornaviridae AF406813 AAM33242 Sapelovirus A Picornaviridae V01149 CAA24461 Enterovirus C Picornaviridae X96871 CAA65615 Erbovirus A Picornaviridae AF231769 AAK12418 Teschovirus A Picornaviridae JQ941880 AFS33288 Hunnivirus A Picornaviridae Torchivirus KM873611 AIY68181 Torchivirus A Picornaviridae JF973687 AEM05833 Mosavirus A Picornaviridae AY593829 AAT01772 Foot-and-mouth disease virus Picornaviridae FJ438902 ACL15185 Cosavirus A Picornaviridae M81861 AAA43037 Cardiovirus A Picornaviridae JQ814851 AFK85007 Mischivirus A Picornaviridae DQ641257 ABG23522 Senecavirus A Picornaviridae HM751199 ADN94255 Melegrivirus A Picornaviridae JF973686 AEM05832 Rosavirus A Picornaviridae JN819202 AFB77697 Cadicivirus A Picornaviridae GQ179640 ACS32214 Salivirus A Picornaviridae AB040749 BAB62889 Aichivirus A Picornaviridae GU182408 ADL38959 Oscivirus A Picornaviridae Sakobuvirus KF387721 AGZ63355 Sakobuvirus A Picornaviridae GU182406 ADL38957 Passerivirus A Picornaviridae Sicinivirus KF741227 AHN05554 Sicinivirus A Picornaviridae JQ691613 AFO66657 Gallivirus A Iflavirus NC_003781 NP620559 Infectious flacherie virus M95497 AAA66056 Rice tungro spherical virus Secoviridae D14066 BAA03151 Parsnip yellow fleck virus Secoviridae DQ388879 ABD38934 Tomato torrado virus Secoviridae AB009958 BAA76746 Satsuma dwarf virus Secoviridae AJ621357 CAF21713 Cherry rasp leaf virus Secoviridae KJ556849 AIA10370 Tobacco ringspot virus Secoviridae AB084450 BAD00183 Broad bean wilt virus 1 Secoviridae X00206 CAA25029 Cowpea mosaic virus NC_003924 NP647481 Cricket paralysis virus Dicistroviridae Triatovirus NC_003783 NP620562 Triatoma virus Dicistroviridae NC_002548 NP066241 Acute bee paralysis virus KX884351 APG78541 APG78542 Wenzhou picorna-like virus 21 KX884356 APG78550 APG78551 Wenzhou picorna-like virus 20 KY130497 ASG92536 ASG92537 N OV 064 KX884333 APG78509 Wenzhou picorna-like virus 11 KX883647 APG77368 Shahe picorna-like virus 14 KX883664 APG77395 Shahe picorna-like virus 14 KX884262 APG78394 APG78395 Hubei picorna-like virus 6 KX883716 APG77477 Sanxia picorna-like virus 7 KX884358 APG78553 APG78554 Wenzhou picorna-llike virus 17 KX883402 APG76853 APG76854 Beihai picorna-like virus 30 KX884389 APG78606 APG78607 Beihai paphia shell virus 2 KX884525 APG78963 APG78964 Beihai paphia shell virus 2 KX883713 APG77473 APG77473 Sanxia picorna-like virus 6 KX883373 APG76803 APG76803 Beihai Picorna-like virus 36 KX883426 APG76901 APG76902 Beihai Picorna-like virus 37 KX884495 APG78914 APG78915 Beihai Picorna-like virus 38 KX884373 APG78578 APG78579 Wenzhou picorna-like virus 53 KX883410 APG76871 APG76872 Beihai picorna-like virus 39 KY130495 ASG92532 ASG92533 N OV 013 KX883667 APG77399 APG77400 Shahe picorna-like virus 3 KY130492 ASG92527 ASG92526 N OV 001 KX884311 APG78476 APG78477 Wenling picorna-like virus 1 KX884372 APG78576 APG78577 Wenzhou gastropodes virus 1 KX883365 APG76787 APG76788 Beihai picorna-like virus 20 KX884369 APG78570 APG78571 Wenzhou picorna-like virus 5 KX884476 APG78883 APG78884 Wenzhou picorna-like virus 5 KX884512 APG78942 APG78943 Beihai paphia shell virus 1 KX884527 APG78967 APG78968 Beihai paphia shell virus 1 Supplementary Table 3: Mean p-value distances between genera in the Marnaviridae.

RdRp Salisharnavirus Bacillarnavirus Kusarnavirus Sogarnavirus Locarnavirus Labyrnavirus Marnavirus

Marnavirus 0.75 0.72 0.69 0.72 0.70 0.71 Labyrnavirus 0.76 0.69 0.73 0.73 0.73 0.73 Locarnavirus 0.76 0.76 0.53 0.60 0.66 0.61 Kusarnavirus 0.72 0.73 0.72 0.55 0.63 0.56 capsid Bacillarnavirus 0.72 0.75 0.73 0.70 0.61 0.54 Salisharnavirus 0.71 0.73 0.72 0.66 0.71 0.55 Sogarnavirus 0.71 0.74 0.72 0.58 0.70 0.66 Supplementary Table 4: Viruses where both proteins did not fall within the species demarcation.

Virus 1 Virus 2 Amino acid pairwise identity

Genome RdRp Capsid Virus Genome RdRp Capsid Virus Capsid (75) RdRp (90) KX884370 APG78572 APG78573 Wenzhou picorna-like virus 1 KX884519 APG78954 APG78955 Beihai picorna-like virus 11 88.33 76.34 KX884501 APG78922 APG78923 Beihai sipunculid worm virus 3 KX883404 APG76858 APG76859 Beihai sipunculid worm virus 2 83.15 70.11 KX883323 APG76710 APG76711 Beihai picorna-like virus 35 KX884376 APG78583 APG78584 Wenzhou picorna-like virus 10 85.26 75.65 KX884499 APG78919 APG78920 Beihai picorna-like virus 31 KX883404 APG76858 APG76859 Beihai sipunculid worm virus 2 32.25 98.14 KX883420 APG76890 APG76891 Beihai picorna-like virus 35 KX883902 APG77928 APG77929 Beihai picorna-like virus 34 4.93 90.33 KX884527 APG78967 APG78968 Beihai paphia shell virus 1 KX884369 APG78570 APG78571 Wenzhou picorna-like virus 5 62.01 92.02 KX884510 APG78938 APG78939 Beihai picorna-like virus 4 AB639040 BAK40203 BAK40204 Chaetoceros sp. RNA virus 2 51.58 98.13 Supplementary Figure 1: Pairwise capsid protein sequence comparisons of viruses in the family Marnaviridae. Jukes-Cantor distances and percent amino acid similarities were analyzed: black indicates the highest similarity detected (lowest distance score), and white the lowest similarity (highest distance score). Colours denote genera: Marnavirus (blue), Labyrnavirus (red), Locarnavirus (purple), Kusarnavirus (yellow), Bacillarnavirus (pink), Salisharnavirus (orange), Sogarnavirus (green) and the type species of the genus Dicistrovirus, family Dicistroviridae (Cricket paralysis virus ) (white).

Percent amino acid identity CtenRNAV typeII CtenRNAV CtenRNAV01 CspRNAV2 AglaRNAV CsfrRNAV AuRNAV HaRNAV RsRNAV PAL473 PAL128 PAL156 CrPV BC-4 BC-1 BC-3 Capsid BC-2 JP-B SF-2 SF-1 SF-3 JP-A

Heterosigma akashiwo RNA virus 19.7 20.2 21.3 21.7 20.3 23.9 24.5 24.8 22.8 26.0 24.1 22.2 18.5 23.5 23.7 22.8 23.4 26.4 23.3 16.6 Aurantiochytrium single-stranded RNA virus 1.5 20.8 18.2 19.5 17.2 21.7 18.9 21.7 18.3 20.8 21.2 21.5 18.2 22.5 20.9 21.6 20.0 20.8 17.1 16.0 JP-B 1.6 1.4 32.5 33.5 34.3 26.0 24.2 26.2 23.9 26.3 22.1 25.5 21.3 25.6 27.4 25.9 22.8 25.1 21.8 17.4 SF-2 1.5 1.6 1.1 35.1 32.2 23.6 23.6 25.2 22.6 21.6 20.2 22.1 20.8 25.4 24.4 24.6 20.8 23.3 20.2 14.9 SF-1 1.5 1.5 1.1 1.0 38.3 23.3 23.6 26.1 22.8 24.2 22.6 25.2 19.6 23.1 25.9 25.5 21.6 23.8 20.3 16.7 SF-3 1.6 1.6 1.0 1.1 0.9 24.7 23.6 23.5 23.2 22.5 22.0 21.9 20.0 23.6 25.0 22.7 21.7 23.1 21.5 15.8 Asterionellopsis glacialis RNA virus 1.4 1.4 1.3 1.4 1.4 1.3 26.7 28.1 25.6 30.4 28.7 28.1 24.0 46.3 44.3 47.9 28.5 32.8 29.2 16.7 Chaetoceros tenuissimus RNA virus 01 1.3 1.5 1.4 1.4 1.4 1.4 1.3 33.4 30.8 25.2 25.6 24.7 20.4 27.7 27.2 26.8 24.6 26.8 24.1 16.4 Rhizosolenia setigera RNA virus 1.3 1.4 1.3 1.3 1.3 1.4 1.2 1.1 29.1 26.7 28.0 27.7 21.9 28.1 29.4 27.3 27.0 28.8 24.3 15.3 Chaetoceros socialis f radians RNA virus 1.4 1.6 1.4 1.4 1.4 1.4 1.3 1.1 1.2 24.3 22.5 23.0 19.7 26.7 26.4 26.2 22.3 23.9 20.4 17.5 BC-4 1.3 1.4 1.3 1.5 1.4 1.4 1.1 1.3 1.3 1.4 33.0 34.4 24.9 29.4 29.2 30.5 25.2 30.0 25.4 16.1 PAL473 1.3 1.5 1.4 1.5 1.4 1.4 1.1 1.3 1.2 1.4 0.9 31.4 26.0 30.6 29.0 29.9 30.9 31.5 26.4 15.2 BC-1 1.4 1.3 1.2 1.4 1.3 1.4 1.1 1.3 1.2 1.4 0.9 0.9 34.4 28.9 30.3 30.5 28.0 29.8 25.5 14.5

Jukes-Cantor Distance Jukes-Cantor PAL128 1.4 1.3 1.3 1.3 1.4 1.3 1.2 1.4 1.3 1.4 1.1 1.0 0.9 24.3 25.4 25.6 21.7 24.0 20.4 13.2 BC-2 1.4 1.3 1.3 1.3 1.4 1.4 0.7 1.3 1.2 1.3 1.2 1.1 1.1 1.2 56.9 56.9 28.7 32.0 27.6 18.1 PAL156 1.4 1.4 1.2 1.4 1.3 1.3 0.8 1.3 1.2 1.3 1.2 1.1 1.1 1.2 0.5 59.0 30.2 31.8 28.7 17.3 BC-3 1.4 1.4 1.3 1.3 1.3 1.4 0.7 1.3 1.3 1.3 1.1 1.1 1.1 1.1 0.5 0.5 28.1 32.1 28.1 17.5 JP-A 1.3 1.6 1.3 1.4 1.4 1.4 1.2 1.3 1.2 1.4 1.2 1.1 1.1 1.2 1.1 1.1 1.1 48.6 43.9 17.5 Chaetoceros tenuissimus RNA virus typeII 1.2 1.5 1.3 1.4 1.4 1.4 1.1 1.3 1.2 1.4 1.1 1.1 1.1 1.2 1.1 1.1 1.1 0.6 46.4 18.7 Chaetoceros sp. RNA virus 2 1.2 1.5 1.3 1.4 1.4 1.3 1.1 1.2 1.2 1.4 1.1 1.1 1.1 1.2 1.1 1.1 1.1 0.6 0.6 14.9 Cricket paralysis virus 1.7 1.8 1.7 1.8 1.7 1.7 1.8 1.8 1.9 1.7 1.8 1.8 1.8 1.8 1.7 1.7 1.7 1.7 1.7 1.8 Supplementary Figure 2: Pairwise RdRp amino acid sequence comparisons of viruses in the family Marnaviridae. Jukes-Cantor distances and percent amino acid similarities were analyzed; black indicates the highest similarity detected (lowest distance score), and white the lowest similarity (Highest distance score). Colours denote genera: Marnavirus (blue), Labyrnavirus (red), Locarnavirus (purple), Kusarnavirus (yellow), Bacillarnavirus (pink), Salisharnavirus (orange), Sogarnavirus (green) and the type species of the genus Dicistrovirus, family Dicistroviridae (Cricket paralysis virus) (white).

Percent amino acid identity CtenRNAV typeII CtenRNAV CtenRNAV01 CspRNAV2 AglaRNAV CsfrRNAV AuRNAV HaRNAV RsRNAV PAL128 PAL156 PAL473 CrPV BC-4 BC-1 BC-2 BC-3 SF-2 SF-1 JP-A JP-B RdRp SF-3

Heterosigma akashiwo RNA virus 22.4 29.2 24.0 27.2 24.7 29.5 26.6 24.1 24.5 25.8 29.2 30.3 27.4 25.0 27.9 27.5 28.9 27.8 27.0 27.7 Aurantiochytrium single-stranded RNA virus 1.5 26.8 28.6 29.7 33.6 25.4 24.4 23.8 24.5 25.4 24.7 25.4 24.3 25.6 27.1 26.8 24.3 25.7 24.3 24.0 JP-B 1.3 1.3 52.4 54.1 59.1 48.2 41.6 38.9 37.1 36.6 36.0 33.5 34.7 39.7 40.6 39.9 42.4 38.3 41.7 30.0 SF-2 1.5 1.3 0.7 53.5 57.5 45.2 36.6 35.8 38.7 33.8 35.1 29.2 28.4 31.9 35.6 35.2 35.8 34.3 39.1 28.6 SF-1 1.3 1.2 0.6 0.6 58.4 45.6 34.4 36.5 36.5 33.5 33.2 31.4 32.8 33.7 38.1 36.7 39.1 36.1 39.5 29.6 SF-3 1.4 1.1 0.5 0.6 0.5 45.2 36.2 35.8 38.3 32.7 32.8 29.5 28.4 33.0 35.9 36.3 36.2 33.9 36.5 27.9 Asterionellopsis glacialis RNA virus 1.2 1.4 0.7 0.8 0.8 0.8 47.8 42.1 39.6 35.6 35.9 35.2 36.4 42.7 42.5 41.7 43.1 42.4 46.1 31.4 Chaetoceros tenuissimus RNA virus 01 1.3 1.4 0.8 1.0 1.0 1.0 0.7 62.1 57.8 37.9 39.2 38.6 39.7 43.0 46.7 44.6 47.8 43.5 47.5 24.0 Rhizosolenia setigera RNA virus 1.4 1.4 0.9 1.0 1.0 1.0 0.8 0.5 64.4 35.7 39.1 37.7 38.0 43.8 45.3 44.6 44.3 41.1 44.3 24.4 Chaetoceros socialis f radians RNA virus 1.4 1.4 0.9 0.9 1.0 0.9 0.9 0.5 0.5 32.5 37.0 34.4 36.6 41.0 38.8 41.9 40.4 37.6 40.0 24.4 BC-4 1.4 1.4 1.0 1.1 1.1 1.1 1.1 1.0 1.0 1.1 63.6 35.6 38.9 39.6 41.2 39.1 44.4 40.4 43.3 24.9 PAL473 1.2 1.4 1.0 1.1 1.1 1.1 1.0 0.9 0.9 1.0 0.4 43.0 43.0 42.5 45.2 40.9 46.1 44.5 47.2 24.9 BC-1 1.2 1.4 1.1 1.3 1.2 1.2 1.1 1.0 1.0 1.1 1.0 0.9 60.4 43.9 45.2 43.4 46.1 41.5 43.9 26.3

Jukes-Cantor Distance Jukes-Cantor PAL128 1.3 1.4 1.1 1.3 1.1 1.3 1.0 0.9 0.9 1.0 0.9 0.9 0.5 42.7 46.8 44.2 42.2 39.5 41.8 24.3 BC-2 1.4 1.4 0.9 1.1 1.1 1.1 0.8 0.8 0.8 0.8 0.9 0.8 0.8 0.8 69.9 67.7 53.4 48.8 55.6 24.0 PAL156 1.3 1.4 0.9 1.0 0.9 1.0 0.9 0.7 0.7 0.9 0.9 0.8 0.8 0.7 0.4 77.0 56.1 52.5 57.2 25.8 BC-3 1.3 1.4 0.9 1.0 1.0 1.0 0.9 0.8 0.7 0.8 0.9 0.9 0.8 0.8 0.4 0.3 55.4 49.3 56.8 25.1 JP-A 1.3 1.4 0.9 1.1 1.0 1.0 0.9 0.7 0.8 0.9 0.8 0.8 0.8 0.9 0.6 0.6 0.6 62.4 75.8 26.8 Chaetoceros tenuissimus RNA virus typeII 1.3 1.4 1.0 1.1 1.0 1.1 0.9 0.8 0.9 0.9 0.9 0.8 0.9 0.9 0.7 0.6 0.7 0.5 67.2 29.3 Chaetoceros sp. RNA virus 2 1.3 1.4 0.9 1.0 0.9 1.0 0.8 0.7 0.8 0.9 0.8 0.8 0.8 0.9 0.6 0.5 0.5 0.3 0.4 28.2 Cricket paralysis virus 1.3 1.5 1.2 1.3 1.2 1.3 1.2 1.4 1.4 1.4 1.4 1.4 1.3 1.4 1.5 1.4 1.4 1.3 1.3 1.3 Supplementary Figure 3: Pairwise amino acid comparisons of the RdRp sequence domains of the viruses in the family Marnaviridae and those of the type members of the order . Families are indicated by colours: Potyviridae (dark green), Picornaviridae (red), Iflaviridae (yellow), Dicistroviridae (orange), Secoviridae (lime green) and Marnaviridae (blue). Dark colours indicate lower distances and higher identity values. Protein Identity Iflaviridae Potyviridae Picornaviridae Dicistro Secoviridae viridae Marnaviridae

0.7 52.3 36.9 46.9 48.5 48.3 18.3 15.9 16.4 17.1 19.9 16.6 19.3 19.9 18.8 20.2 16.0 23.8 16.0 18.1 13.3 17.4 18.5 18.3 17.3 16.1 18.9 16.7 18.3 16.6 17.9 18.1 18.1 18.9 20.1 16.7 14.8 17.5 17.4 18.6 18.7 18.6 19.2 19.3 20.1 20.5 17.9 18.0 19.0 18.3 18.0 19.9 20.4 18.2 16.1 18.8 19.2 14.7 16.6 16.7 16.6 15.3 15.0 13.5 16.3 14.8 15.2 14.8 16.2 16.2 14.6 15.9 17.5 0.7 0.6 38.2 41.7 46.0 49.0 18.5 16.6 15.6 17.2 19.5 16.7 17.3 20.4 18.4 21.3 16.0 24.4 17.6 21.2 16.9 21.2 18.9 19.3 20.2 21.4 21.2 19.4 19.0 17.4 18.2 18.6 19.6 20.5 20.4 19.3 20.5 20.1 20.0 19.3 20.5 17.8 18.9 18.6 18.7 18.8 20.1 18.4 17.2 18.5 18.4 22.4 18.9 13.7 17.6 20.7 19.7 17.8 20.1 19.1 17.4 17.6 16.5 14.7 16.0 19.4 18.7 16.0 18.1 17.4 17.3 18.9 17.7 0.8 1.0 0.9 41.4 36.4 41.0 17.1 15.7 17.7 15.7 19.6 17.9 16.2 18.0 15.8 17.6 16.9 21.9 13.9 17.8 15.4 16.1 15.4 16.5 17.2 16.0 18.4 16.9 14.7 14.2 14.6 15.6 17.5 16.5 14.9 17.6 18.0 16.5 16.7 17.8 16.3 18.9 20.3 17.9 19.8 18.8 19.5 20.1 17.9 19.6 17.3 17.3 16.7 15.4 16.2 19.9 21.0 18.0 18.1 16.7 18.1 18.3 17.5 15.6 17.5 14.6 15.3 17.3 16.6 16.9 17.2 17.0 18.6 0.7 0.7 0.9 0.9 51.6 50.2 16.6 18.2 21.1 18.3 21.7 20.0 17.1 19.9 20.2 24.0 20.8 22.5 16.0 20.3 17.1 18.9 18.3 18.3 17.8 17.7 18.2 17.2 17.6 17.7 17.9 16.5 17.0 17.1 17.4 18.3 17.1 17.5 17.1 16.3 18.1 16.1 18.6 20.1 19.5 20.7 20.6 18.9 18.8 17.5 17.7 17.4 19.3 15.3 17.3 23.3 21.6 19.6 18.6 17.2 19.0 16.0 17.7 16.1 15.5 14.0 14.4 17.0 18.1 17.4 16.5 17.1 17.7 0.8 0.7 0.7 1.0 0.6 58.4 18.4 17.3 20.2 19.2 22.7 17.2 19.2 22.0 19.3 22.6 19.9 23.2 15.8 19.0 16.9 18.7 17.9 16.8 15.6 17.5 18.4 17.3 18.1 18.3 18.1 17.8 16.8 18.0 16.1 17.3 16.9 16.5 18.0 16.5 19.6 19.4 19.6 19.1 17.0 20.7 19.5 18.4 19.1 18.4 19.2 20.0 19.9 17.1 19.1 21.0 21.9 18.8 21.1 21.9 16.0 17.6 17.6 15.0 15.8 17.2 16.5 17.3 16.6 17.7 17.6 15.6 18.3 0.8 0.7 0.7 0.9 0.7 0.5 19.1 17.6 19.7 17.2 24.5 18.2 16.9 21.9 18.6 21.4 19.2 24.6 17.6 20.1 17.6 19.4 19.4 19.4 19.0 19.7 20.5 22.9 21.0 20.5 19.9 17.8 20.1 19.4 18.3 20.2 19.5 19.1 18.3 17.5 20.6 19.4 18.9 18.7 17.7 19.2 19.9 19.9 18.3 20.9 18.4 18.9 19.1 16.4 17.0 21.8 21.1 17.7 20.7 21.5 17.8 18.0 17.6 17.2 16.5 16.1 15.8 17.3 17.7 18.4 18.0 15.6 18.0 1.9 1.7 1.7 1.8 1.8 1.7 1.7 16.7 19.4 19.8 19.9 19.0 16.7 20.4 18.9 24.0 21.2 26.6 22.6 19.7 24.7 21.2 23.8 21.4 21.7 22.2 21.9 24.0 22.7 21.5 24.7 22.0 20.4 20.9 21.8 20.9 20.1 20.5 21.9 22.6 23.8 19.5 24.7 20.6 20.0 25.9 20.7 19.5 20.3 21.9 21.2 21.5 22.6 20.3 15.4 20.7 23.9 19.2 21.4 20.1 17.3 19.1 18.7 20.1 18.8 19.1 18.8 19.8 20.5 22.2 22.3 20.7 20.6 1.7 1.9 1.8 1.9 1.7 1.8 1.7 1.8 41.4 34.7 32.0 36.5 30.7 32.2 30.3 23.7 26.8 26.5 27.1 27.7 27.5 29.8 25.7 24.6 26.7 25.9 25.8 28.1 25.4 24.5 27.2 26.6 28.6 28.4 26.7 30.3 27.2 27.2 25.5 25.9 24.9 18.6 20.2 22.5 22.6 22.4 18.8 20.6 19.7 19.5 21.2 21.0 22.2 20.3 19.7 23.5 22.9 25.0 22.1 20.4 18.7 18.9 18.5 21.1 20.4 20.0 19.3 19.0 19.0 19.4 21.2 21.3 20.4 1.8 1.9 1.9 1.7 1.6 1.6 1.6 1.6 0.9 31.4 36.6 36.3 28.2 30.3 37.1 29.1 26.9 29.0 28.4 28.2 27.6 25.3 24.7 24.8 24.7 25.8 26.4 26.5 24.8 25.9 25.1 24.9 23.4 23.8 24.7 25.4 27.3 23.8 23.8 24.5 25.6 20.0 21.2 19.9 22.5 20.5 18.7 19.9 17.8 20.5 19.4 20.9 20.7 18.0 18.6 20.9 21.3 19.8 22.1 19.6 16.8 18.4 18.7 18.8 19.9 17.7 15.8 19.0 17.9 18.6 19.3 18.0 18.2 1.7 1.8 1.8 1.9 1.7 1.7 1.8 1.6 1.1 1.1 39.3 37.0 30.2 35.6 33.1 27.8 26.8 27.6 28.5 26.9 28.1 25.1 25.0 22.8 21.9 24.4 26.6 24.6 21.5 21.8 22.2 27.0 28.2 26.0 26.8 24.1 24.1 25.3 22.5 25.6 24.4 19.4 21.4 18.6 23.0 22.8 18.8 19.0 20.9 20.3 17.7 21.4 22.3 22.2 18.4 25.5 21.1 22.9 21.5 19.3 18.8 19.3 16.1 20.8 19.3 19.0 20.5 21.2 22.3 21.9 22.7 19.9 20.5 1.4 1.6 1.6 1.6 1.5 1.5 1.4 1.6 1.1 1.0 0.9 40.7 33.6 39.5 43.0 30.0 27.8 29.8 29.7 29.6 29.0 23.9 28.4 27.5 22.6 24.1 25.4 25.9 22.7 23.0 21.8 25.9 27.0 27.5 26.0 23.6 26.0 22.5 23.2 24.0 24.1 20.1 21.3 20.7 21.9 25.6 20.9 19.2 20.8 20.6 17.7 22.1 23.6 21.0 19.0 21.7 21.4 21.7 19.9 20.0 20.6 21.1 19.3 19.6 20.1 19.3 19.7 21.2 21.1 20.4 23.0 22.0 23.4 1.6 1.9 1.9 1.7 1.6 1.8 1.7 1.6 1.0 1.0 1.0 0.9 32.4 41.0 39.5 30.1 33.1 30.2 30.4 32.3 32.8 28.6 27.2 27.0 25.0 24.4 27.0 27.7 27.7 26.5 28.8 27.2 29.0 30.3 27.7 26.3 29.4 29.0 29.6 26.9 27.3 18.6 21.3 19.6 22.6 20.9 19.1 19.6 17.9 22.3 19.1 21.0 21.1 21.0 21.5 23.2 20.3 20.2 21.4 19.6 20.5 20.6 19.2 19.6 19.6 20.4 20.7 19.7 20.4 19.7 21.9 18.8 19.3 1.6 1.6 1.7 1.8 1.8 1.6 1.8 1.8 1.2 1.3 1.2 1.1 1.1 29.5 31.5 23.3 22.6 25.8 27.7 24.9 23.4 23.8 25.1 24.1 20.9 25.9 23.7 24.1 21.4 20.5 22.1 24.5 24.2 25.1 23.1 24.7 25.5 22.4 23.1 22.7 22.2 20.1 19.5 17.1 20.8 18.1 18.4 17.8 19.3 18.8 18.4 21.0 21.1 17.3 18.0 18.0 17.0 17.7 18.1 17.1 16.1 17.0 15.3 18.2 19.9 18.5 17.0 18.0 19.0 18.3 18.6 18.1 18.3 1.5 1.6 1.6 1.7 1.6 1.5 1.5 1.6 1.1 1.2 1.0 0.9 0.9 1.2 40.5 30.9 27.7 29.7 26.2 27.2 27.0 26.2 26.1 26.2 25.6 27.9 25.7 26.0 24.5 22.6 24.5 26.8 30.4 25.9 25.0 25.5 27.8 28.9 26.9 27.2 26.1 19.7 21.6 18.6 24.7 23.1 23.1 21.7 20.8 23.3 22.2 21.4 22.2 21.7 19.0 23.1 22.8 21.7 21.0 20.0 20.8 18.8 18.8 20.7 19.0 19.0 17.6 22.2 20.8 19.0 22.2 20.9 22.6 1.5 1.7 1.7 1.9 1.6 1.7 1.7 1.7 1.2 1.0 1.1 0.8 0.9 1.1 0.9 28.3 30.6 29.6 27.4 25.4 25.5 25.1 24.3 25.1 24.7 26.4 23.4 24.5 23.9 24.9 24.9 24.9 28.2 26.4 26.9 26.7 27.5 27.1 26.6 27.0 26.8 22.3 19.2 21.2 21.2 23.5 21.7 19.3 20.5 20.9 20.1 20.2 20.7 19.9 20.1 19.2 19.6 18.8 21.1 19.3 21.4 21.5 21.1 19.7 19.1 18.7 22.0 19.7 20.9 21.2 22.0 20.3 21.6 1.5 1.6 1.6 1.8 1.4 1.5 1.6 1.4 1.4 1.2 1.2 1.2 1.2 1.4 1.1 1.2 41.4 31.3 29.4 31.3 31.3 27.2 30.4 25.5 27.6 27.7 27.1 29.7 28.3 25.5 28.7 26.5 29.0 27.9 26.1 28.6 27.8 28.2 31.9 29.6 29.9 22.0 22.6 23.1 27.9 22.2 23.3 22.8 20.7 22.3 24.9 23.1 24.0 21.2 19.9 22.3 23.1 21.5 23.4 21.7 23.0 22.7 21.3 20.6 20.7 18.9 19.6 19.2 22.0 22.4 21.0 23.3 23.6 1.7 1.9 1.9 1.9 1.6 1.7 1.7 1.6 1.3 1.3 1.3 1.3 1.1 1.5 1.3 1.1 0.9 31.6 29.1 31.2 29.8 30.1 27.1 27.3 27.4 24.3 26.7 29.2 28.7 24.3 27.4 25.9 27.3 28.2 26.3 29.7 26.7 31.6 30.0 29.3 30.7 19.3 24.7 22.8 24.3 24.1 23.7 20.8 20.3 20.5 18.7 20.6 21.4 19.4 19.6 25.2 24.2 22.3 22.3 21.3 21.5 20.9 22.3 21.3 21.7 19.2 20.7 19.3 20.3 19.9 21.4 22.6 24.3 1.5 1.4 1.4 1.6 1.5 1.5 1.4 1.3 1.3 1.2 1.3 1.2 1.2 1.3 1.2 1.2 1.1 1.2 31.2 30.3 30.4 30.3 28.4 30.5 32.1 30.9 30.3 28.5 28.0 29.0 29.5 30.2 30.5 31.7 31.0 29.9 28.7 26.1 31.8 31.4 30.0 22.3 25.2 20.7 25.8 25.0 23.1 22.7 20.8 22.3 20.9 21.8 20.4 19.2 19.3 21.0 22.1 19.5 22.1 20.0 16.0 17.3 17.6 15.6 19.7 19.7 18.3 17.7 17.6 16.6 17.5 19.9 18.3 1.7 1.9 1.8 2.1 1.9 1.9 1.7 1.4 1.3 1.2 1.3 1.2 1.2 1.2 1.3 1.3 1.2 1.2 1.2 53.9 60.5 37.6 38.4 35.0 35.2 37.0 34.8 36.3 32.6 34.8 32.6 40.0 33.9 34.3 32.0 36.8 38.7 34.3 36.3 37.8 35.7 19.3 22.8 18.6 21.2 22.5 21.4 19.2 19.8 16.4 16.6 22.1 21.5 21.3 20.4 17.0 19.3 18.8 18.5 19.7 16.7 17.9 16.9 18.6 21.2 20.1 19.7 20.1 20.1 20.1 21.3 21.4 22.0 1.5 1.7 1.6 1.7 1.6 1.7 1.6 1.6 1.3 1.2 1.3 1.2 1.1 1.3 1.3 1.3 1.1 1.1 1.2 0.6 55.0 39.9 39.1 37.6 31.7 31.0 36.1 37.6 35.3 34.9 33.5 33.5 37.8 35.9 32.2 32.1 34.0 34.9 35.3 33.7 32.8 20.0 22.0 21.8 21.5 22.4 23.5 20.9 18.2 18.4 20.1 22.1 22.6 21.7 19.0 19.6 21.5 19.2 18.2 20.4 18.1 17.9 20.1 19.3 19.3 18.6 21.6 19.7 19.8 19.1 20.2 21.0 19.4 1.7 2.2 1.9 1.9 1.8 1.8 1.8 1.3 1.3 1.3 1.3 1.2 1.1 1.4 1.3 1.4 1.1 1.2 1.2 0.5 0.5 37.2 36.5 34.7 32.4 33.9 32.8 34.4 31.8 30.9 34.5 38.4 37.8 37.4 32.8 37.6 38.7 36.2 35.1 37.5 34.6 18.3 21.0 18.6 21.2 23.9 23.9 19.2 19.1 18.8 18.0 21.4 20.8 21.3 18.3 18.8 20.0 20.3 20.0 20.4 18.8 21.2 18.3 20.1 18.6 18.6 19.0 19.7 19.4 20.5 21.6 19.6 20.5 1.6 1.8 1.6 1.9 1.7 1.7 1.6 1.5 1.2 1.4 1.4 1.5 1.3 1.4 1.4 1.4 1.3 1.2 1.2 1.0 0.9 1.0 41.4 44.1 38.8 38.4 39.1 39.1 45.1 43.8 41.2 36.2 37.4 35.4 36.7 41.3 38.6 38.6 37.5 37.5 35.7 18.6 23.1 18.3 21.9 21.4 22.1 18.8 18.3 19.2 21.9 21.8 22.6 19.9 17.3 20.3 22.6 20.3 22.6 21.2 17.8 16.9 17.2 17.9 17.1 16.7 20.1 17.3 18.7 18.0 21.3 19.2 18.3 1.6 1.7 1.7 1.9 1.7 1.7 1.6 1.4 1.4 1.4 1.4 1.3 1.3 1.4 1.3 1.5 1.2 1.3 1.2 0.9 0.9 1.0 0.8 50.4 36.8 33.3 34.1 37.6 38.1 39.4 36.6 33.7 37.4 36.3 39.9 36.1 34.5 34.8 36.4 36.4 36.1 18.9 21.6 20.4 21.5 22.7 20.5 20.9 19.3 18.5 17.6 23.5 22.3 20.4 16.1 20.2 20.7 20.2 20.7 22.6 16.6 17.4 20.6 18.9 22.2 19.6 19.6 20.0 19.7 19.7 19.0 20.2 20.8 1.6 1.7 1.6 1.8 1.7 1.8 1.7 1.5 1.4 1.4 1.5 1.3 1.3 1.5 1.3 1.4 1.4 1.3 1.2 1.0 0.9 1.0 0.8 0.7 39.2 40.3 36.8 37.2 38.5 41.1 38.9 33.1 34.4 35.3 33.9 35.4 36.6 35.7 33.9 33.9 35.4 17.5 22.8 21.2 21.9 22.4 19.9 23.8 19.8 19.0 20.9 19.9 23.1 20.1 17.0 18.5 18.9 18.5 19.3 19.0 15.4 14.5 16.0 15.0 14.8 17.0 20.7 18.0 17.0 17.0 18.3 17.8 20.2 1.6 1.8 1.6 1.8 1.8 1.9 1.7 1.5 1.3 1.4 1.6 1.5 1.4 1.6 1.4 1.4 1.3 1.3 1.1 1.0 1.1 1.1 0.9 0.9 0.9 55.6 37.2 43.7 48.4 47.5 45.3 38.0 37.2 31.8 38.4 39.9 39.9 40.5 41.0 39.5 36.2 20.4 22.6 18.2 23.9 22.0 21.3 21.4 22.1 22.2 20.4 22.1 20.0 22.1 17.5 20.5 21.0 21.3 21.3 19.9 19.1 19.2 17.1 17.4 19.9 18.5 21.4 18.3 19.0 18.3 20.0 20.1 17.1 1.7 1.9 1.6 1.9 1.8 1.8 1.6 1.5 1.4 1.4 1.5 1.4 1.5 1.3 1.3 1.3 1.3 1.4 1.2 1.0 1.2 1.1 1.0 1.1 0.9 0.6 39.0 40.9 46.1 45.0 40.3 40.6 38.0 33.0 37.3 41.9 41.9 40.7 40.2 39.1 38.0 20.4 20.9 21.1 20.4 21.7 23.6 23.1 21.5 20.8 19.7 24.3 20.0 17.6 19.7 21.3 22.5 21.3 23.6 20.0 16.1 17.0 16.3 15.6 17.3 17.3 19.1 18.3 18.7 18.3 18.6 19.5 19.0 1.7 1.7 1.6 1.7 1.8 1.7 1.6 1.5 1.4 1.3 1.4 1.4 1.4 1.4 1.4 1.5 1.3 1.3 1.2 1.1 1.0 1.1 1.0 1.0 1.0 1.0 1.0 46.3 43.5 38.9 45.7 34.8 31.1 31.6 35.7 35.4 35.8 35.8 32.3 34.2 36.0 20.4 20.6 18.3 21.6 22.5 21.7 22.8 19.8 17.8 18.8 24.0 24.1 19.9 18.3 21.4 21.1 21.4 23.0 21.2 18.1 18.9 19.3 19.7 20.1 20.1 21.9 16.6 18.7 19.1 18.0 17.8 17.6 1.7 1.9 1.7 1.8 1.9 1.8 1.4 1.4 1.3 1.3 1.4 1.4 1.3 1.4 1.4 1.4 1.2 1.2 1.3 1.0 1.0 1.1 0.9 0.9 0.9 0.8 0.9 0.8 53.4 50.8 51.6 33.7 34.2 34.8 33.9 34.2 35.0 34.4 33.5 31.9 33.0 20.8 23.8 22.2 23.7 24.3 22.4 19.9 20.2 20.6 19.2 21.9 19.7 21.5 18.7 19.9 20.7 19.9 21.5 20.4 17.0 16.8 17.1 17.9 19.0 17.8 16.7 18.4 18.7 18.4 18.7 17.0 17.2 1.6 1.8 1.7 2.0 1.8 1.7 1.6 1.5 1.4 1.4 1.6 1.5 1.3 1.6 1.4 1.4 1.3 1.2 1.3 1.1 1.0 1.2 0.8 0.9 0.9 0.7 0.8 0.8 0.6 58.6 60.6 34.4 33.2 34.1 36.1 39.1 37.1 36.7 36.3 36.3 34.2 21.2 19.6 17.6 22.4 20.3 19.9 19.9 21.0 21.3 21.6 22.6 23.7 20.0 18.4 20.7 21.2 19.3 20.8 19.4 17.8 18.6 18.3 18.0 19.4 18.7 19.8 17.3 19.9 18.8 19.5 19.6 17.3 1.8 1.9 1.8 2.1 1.8 1.7 1.6 1.5 1.5 1.4 1.6 1.5 1.4 1.6 1.5 1.4 1.4 1.4 1.3 1.1 1.1 1.2 0.8 0.9 0.9 0.7 0.8 1.0 0.7 0.5 59.6 35.9 33.6 33.6 35.3 37.5 34.4 36.5 35.1 33.6 33.1 20.7 21.6 19.6 21.5 20.2 20.7 18.4 19.7 18.8 20.8 22.8 20.4 19.4 16.9 16.9 19.6 21.3 18.5 16.4 15.2 16.1 16.8 16.0 18.5 17.4 18.5 14.4 16.2 16.6 17.5 17.0 16.8 1.7 1.8 1.8 2.0 1.8 1.8 1.6 1.3 1.3 1.4 1.6 1.5 1.3 1.5 1.4 1.4 1.2 1.3 1.2 1.1 1.1 1.1 0.9 1.0 0.9 0.8 0.9 0.8 0.7 0.5 0.5 34.8 37.1 36.7 36.1 36.3 36.3 36.5 33.2 32.1 35.0 21.2 22.1 19.4 21.9 22.1 19.6 18.4 21.3 20.6 20.9 22.3 20.5 20.0 19.4 21.0 22.2 22.1 23.3 20.8 17.8 17.6 19.0 17.2 18.2 19.0 18.2 16.9 20.5 20.1 19.4 21.0 18.0 1.8 1.8 1.7 1.9 1.9 1.7 1.7 1.5 1.4 1.4 1.3 1.4 1.3 1.4 1.3 1.4 1.3 1.4 1.2 0.9 1.1 1.0 1.0 1.0 1.1 1.0 0.9 1.1 1.1 1.1 1.0 1.0 42.0 41.3 39.8 44.7 45.9 43.0 41.4 42.5 40.4 22.9 22.9 19.2 21.4 21.9 21.2 21.2 22.0 20.1 19.0 23.1 19.9 17.7 19.6 20.2 17.3 20.5 19.5 19.9 16.2 18.2 16.7 16.3 17.3 16.6 17.3 18.3 17.2 19.0 20.7 19.8 21.1 1.8 1.8 1.7 1.8 1.9 1.8 1.6 1.6 1.3 1.5 1.3 1.3 1.3 1.4 1.2 1.3 1.2 1.3 1.2 1.1 1.0 1.0 1.0 1.0 1.1 1.0 1.0 1.2 1.1 1.1 1.1 1.0 0.9 64.2 40.6 44.5 44.1 42.9 44.0 43.6 42.2 18.2 20.2 21.8 23.7 23.5 25.4 23.8 21.2 21.5 21.1 20.6 21.5 22.0 20.1 23.2 22.9 21.7 21.4 21.5 18.4 18.9 19.6 18.6 18.9 19.6 21.5 21.4 21.5 21.8 22.6 20.9 21.2 1.9 1.7 1.6 1.9 1.9 1.8 1.6 1.6 1.3 1.5 1.4 1.3 1.2 1.4 1.4 1.3 1.3 1.3 1.1 1.1 1.0 1.0 1.1 1.0 1.0 1.2 1.1 1.2 1.1 1.1 1.1 1.0 0.9 0.5 38.3 39.4 39.8 37.4 40.5 38.2 38.8 18.6 23.5 20.1 23.4 22.0 20.3 20.6 23.8 20.9 18.4 21.3 19.3 20.6 20.1 22.9 20.7 21.0 21.5 21.9 17.3 18.2 16.8 19.0 19.3 20.8 20.5 19.4 19.4 20.5 21.3 22.5 20.5 1.7 1.6 1.6 2.0 1.8 1.9 1.7 1.5 1.4 1.4 1.4 1.4 1.3 1.5 1.4 1.3 1.3 1.3 1.2 1.1 1.1 1.1 1.0 0.9 1.1 0.9 1.0 1.0 1.1 1.0 1.1 1.0 0.9 0.9 1.0 54.7 46.1 50.8 47.9 48.7 50.2 20.4 19.8 19.6 21.1 20.6 22.0 18.8 21.5 19.8 17.6 21.0 20.0 19.9 19.0 22.7 24.3 23.8 23.5 19.9 17.6 17.1 19.6 20.3 19.6 19.2 21.8 20.1 21.4 20.7 22.2 21.2 20.7 1.7 1.9 1.7 1.8 1.8 1.8 1.6 1.5 1.2 1.4 1.5 1.5 1.4 1.4 1.4 1.3 1.2 1.2 1.2 1.0 1.1 1.0 0.9 1.0 1.0 0.9 0.9 1.0 1.1 0.9 1.0 1.0 0.8 0.8 1.0 0.6 61.0 59.5 59.5 56.0 55.9 19.4 21.4 20.4 22.7 20.7 23.6 19.6 20.2 15.3 17.3 21.4 18.6 18.5 18.0 20.3 21.5 19.9 19.6 19.0 17.4 16.5 17.9 17.2 17.5 18.6 19.0 19.0 18.4 18.7 20.2 18.5 20.2 1.7 2.0 1.6 1.7 1.8 1.8 1.6 1.6 1.3 1.3 1.5 1.4 1.3 1.4 1.3 1.3 1.3 1.3 1.3 1.0 1.1 1.0 1.0 1.0 1.0 0.9 0.9 1.0 1.0 1.0 1.1 1.0 0.8 0.8 0.9 0.8 0.5 57.5 57.2 55.3 51.3 19.8 19.2 17.2 19.8 22.1 20.0 19.2 19.4 17.4 18.7 19.6 17.2 19.2 18.0 21.4 19.6 19.9 21.1 19.0 18.5 17.6 19.0 17.2 15.2 17.5 18.6 18.3 16.6 17.7 20.9 18.5 19.8 1.7 1.8 1.6 1.9 1.8 1.9 1.7 1.6 1.3 1.5 1.4 1.5 1.3 1.5 1.3 1.3 1.3 1.1 1.4 1.1 1.0 1.0 1.0 1.0 1.0 0.9 0.9 1.0 1.1 1.0 1.0 1.0 0.8 0.9 1.0 0.7 0.5 0.6 62.7 62.3 54.8 20.2 20.3 19.4 23.4 20.7 24.4 19.2 20.5 18.5 18.0 20.3 17.2 21.4 18.0 19.9 20.7 20.7 19.6 18.6 17.8 18.3 19.0 17.5 17.8 15.6 18.2 19.0 19.1 19.4 20.5 18.8 20.5 1.7 1.8 1.7 1.8 1.9 1.8 1.7 1.5 1.4 1.4 1.6 1.5 1.2 1.5 1.3 1.3 1.1 1.2 1.1 1.0 1.0 1.0 1.0 1.0 1.1 0.9 0.9 1.1 1.1 1.0 1.1 1.1 0.9 0.8 0.9 0.7 0.5 0.5 0.5 66.5 63.6 17.2 21.0 20.1 24.0 20.9 23.8 21.0 20.8 18.5 18.4 21.7 18.9 19.6 19.1 19.9 20.0 19.9 19.6 19.7 18.1 17.6 17.9 17.5 18.2 19.0 19.0 18.3 19.1 18.7 20.5 19.6 20.2 1.8 1.8 1.7 1.8 1.9 1.9 1.8 1.5 1.4 1.4 1.4 1.4 1.3 1.5 1.3 1.3 1.2 1.2 1.2 1.0 1.1 1.0 1.0 1.0 1.1 0.9 0.9 1.1 1.1 1.0 1.1 1.2 0.8 0.8 1.0 0.7 0.6 0.6 0.5 0.4 59.8 18.7 20.3 20.1 21.9 21.7 23.5 20.6 20.4 20.9 20.5 22.4 15.6 22.1 17.7 19.2 20.0 18.5 20.0 20.1 18.1 18.3 18.3 16.8 18.2 19.0 19.0 19.0 20.1 19.4 22.0 19.9 21.6 1.7 1.7 1.6 1.9 1.8 1.7 1.6 1.4 1.4 1.4 1.4 1.5 1.3 1.5 1.4 1.3 1.2 1.2 1.2 1.0 1.1 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.1 1.1 1.1 1.0 0.9 0.8 0.9 0.7 0.5 0.6 0.6 0.4 0.5 20.2 23.5 20.8 21.5 22.0 23.1 21.4 20.8 20.6 19.1 20.2 19.3 19.9 19.1 20.3 20.7 20.3 21.9 20.1 16.4 17.7 17.7 19.0 17.6 17.2 19.4 19.4 19.8 19.8 21.3 19.9 21.3 1.8 1.7 1.8 1.7 1.9 1.7 1.7 1.7 1.7 1.6 1.7 1.6 1.7 1.6 1.7 1.5 1.5 1.7 1.5 1.7 1.6 1.7 1.7 1.7 1.8 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.5 1.8 1.7 1.6 1.7 1.7 1.6 1.9 1.8 1.7 21.2 22.1 22.2 22.2 21.6 22.2 20.1 22.3 19.8 24.0 26.3 21.9 20.9 23.7 22.6 23.3 23.7 20.2 22.3 18.5 20.2 17.6 19.5 18.1 17.8 21.0 21.0 19.6 18.8 18.3 19.2 1.7 1.7 1.7 1.6 1.7 1.6 1.7 1.4 1.6 1.5 1.5 1.5 1.5 1.6 1.5 1.6 1.5 1.4 1.3 1.4 1.5 1.5 1.4 1.5 1.4 1.4 1.6 1.6 1.4 1.6 1.5 1.5 1.4 1.6 1.4 1.6 1.5 1.7 1.6 1.6 1.6 1.5 1.6 31.9 46.0 28.5 28.8 26.0 26.3 26.6 22.0 24.8 26.2 27.7 24.0 30.0 28.6 29.6 27.9 31.4 24.0 24.4 24.4 24.9 24.9 26.3 24.3 24.0 25.8 25.1 26.8 29.3 28.2 1.7 1.7 1.7 1.8 1.6 1.7 1.7 1.6 1.4 1.6 1.7 1.5 1.6 1.7 1.7 1.5 1.5 1.5 1.6 1.7 1.5 1.7 1.7 1.5 1.5 1.7 1.6 1.7 1.5 1.8 1.6 1.7 1.6 1.5 1.6 1.6 1.6 1.8 1.6 1.6 1.6 1.6 1.6 1.2 35.4 25.3 25.2 25.4 23.0 24.4 20.4 23.2 24.6 22.7 26.2 22.7 25.9 25.2 23.7 22.1 22.3 19.5 19.9 18.4 20.2 21.3 19.9 20.3 21.8 22.1 20.3 22.5 20.7 1.6 1.6 1.7 1.6 1.7 1.8 1.8 1.6 1.4 1.4 1.4 1.5 1.5 1.5 1.4 1.5 1.3 1.4 1.3 1.5 1.5 1.5 1.5 1.5 1.5 1.4 1.6 1.5 1.4 1.5 1.5 1.5 1.5 1.4 1.4 1.5 1.5 1.6 1.4 1.4 1.5 1.6 1.5 0.7 1.1 30.4 28.3 25.9 26.7 26.3 22.6 27.6 27.6 25.8 22.9 28.1 26.1 25.4 26.8 23.1 21.5 21.3 23.0 20.3 22.4 21.0 19.2 19.3 21.1 20.8 20.6 21.4 19.9 1.7 1.6 1.7 1.7 1.6 1.6 1.7 1.3 1.5 1.6 1.5 1.3 1.6 1.7 1.5 1.4 1.5 1.4 1.4 1.5 1.5 1.4 1.6 1.4 1.5 1.5 1.6 1.5 1.4 1.6 1.6 1.5 1.5 1.4 1.5 1.6 1.6 1.5 1.6 1.6 1.6 1.5 1.6 1.3 1.4 1.2 42.1 36.5 34.9 34.5 29.1 28.7 33.5 24.9 23.4 25.0 25.5 23.3 25.1 25.0 22.4 21.1 20.4 19.6 22.5 22.5 19.6 24.8 24.8 23.4 23.9 24.3 24.6

1.6 1.7 1.6 1.7 1.6 1.7 1.6 1.6 1.7 1.7 1.7 1.6 1.7 1.7 1.5 1.5 1.5 1.4 1.5 1.5 1.4 1.4 1.5 1.5 1.6 1.5 1.5 1.5 1.5 1.6 1.6 1.7 1.5 1.4 1.6 1.5 1.4 1.6 1.4 1.4 1.5 1.5 1.6 1.3 1.4 1.3 0.9 36.4 33.3 31.6 25.1 28.5 34.7 24.4 23.8 23.5 25.7 23.9 26.8 23.5 21.3 20.7 23.1 21.7 22.4 19.2 19.6 21.2 22.3 22.0 23.5 22.8 24.9 Jukes-Cantor Distance Jukes-Cantor 1.7 1.7 1.7 1.6 1.7 1.7 1.6 1.6 1.5 1.6 1.7 1.6 1.6 1.7 1.5 1.6 1.4 1.6 1.5 1.6 1.5 1.6 1.7 1.5 1.4 1.5 1.4 1.5 1.6 1.6 1.7 1.7 1.5 1.4 1.6 1.7 1.6 1.6 1.6 1.6 1.6 1.6 1.5 1.4 1.4 1.4 1.0 1.0 31.7 35.5 32.4 27.3 33.7 26.4 21.7 24.0 23.4 24.8 22.7 23.7 21.4 20.1 19.8 23.3 22.6 19.4 24.0 22.4 24.5 22.4 24.4 22.6 24.7 1.8 1.7 1.8 1.8 1.7 1.7 1.8 1.6 1.6 1.7 1.6 1.6 1.8 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.7 1.7 1.8 1.6 1.6 1.5 1.6 1.7 1.6 1.6 1.7 1.6 1.5 1.6 1.4 1.5 1.6 1.7 1.6 1.6 1.6 1.6 1.7 1.4 1.5 1.4 1.1 1.1 1.2 36.1 27.2 29.2 33.6 21.5 25.1 26.0 27.2 25.4 27.9 24.9 22.9 23.7 23.3 23.8 23.5 22.4 21.7 23.2 25.1 23.7 24.9 23.8 24.9 1.8 1.7 1.7 1.6 1.8 1.7 1.6 1.5 1.6 1.5 1.5 1.5 1.4 1.6 1.4 1.5 1.5 1.6 1.5 1.8 1.7 1.6 1.6 1.6 1.6 1.4 1.5 1.7 1.5 1.5 1.7 1.5 1.6 1.5 1.5 1.6 1.9 1.7 1.7 1.7 1.5 1.6 1.5 1.4 1.4 1.3 1.1 1.1 1.0 1.0 30.5 32.3 38.4 24.2 20.1 28.3 28.0 27.3 29.4 26.6 23.9 23.2 22.6 26.1 24.7 22.3 22.7 24.0 26.0 25.7 25.2 25.5 24.8 1.8 1.7 1.7 1.8 1.8 1.7 1.7 1.6 1.5 1.6 1.7 1.7 1.6 1.6 1.5 1.6 1.4 1.7 1.6 1.8 1.6 1.7 1.5 1.7 1.5 1.6 1.6 1.7 1.7 1.5 1.6 1.5 1.6 1.5 1.7 1.8 1.8 1.7 1.7 1.7 1.6 1.7 1.7 1.6 1.6 1.5 1.2 1.4 1.1 1.3 1.2 33.1 39.7 22.6 19.3 23.8 23.1 23.0 22.3 22.1 22.3 20.6 21.3 22.4 22.5 20.4 24.3 22.1 24.8 23.1 23.6 23.3 22.9 1.7 1.7 1.5 1.8 1.8 1.7 1.7 1.5 1.6 1.5 1.5 1.5 1.6 1.5 1.6 1.6 1.5 1.6 1.6 1.5 1.5 1.6 1.6 1.4 1.6 1.5 1.4 1.4 1.5 1.5 1.5 1.5 1.5 1.6 1.6 1.6 1.6 1.7 1.6 1.6 1.5 1.7 1.5 1.4 1.5 1.3 1.3 1.3 1.3 1.3 1.1 1.1 51.5 19.3 20.7 23.6 26.3 24.1 24.1 21.0 23.1 26.3 21.4 22.8 25.5 23.3 22.6 23.2 25.3 24.9 23.6 24.5 24.7 1.8 1.6 1.7 1.9 1.7 1.7 1.7 1.5 1.5 1.5 1.5 1.4 1.6 1.5 1.5 1.6 1.4 1.6 1.6 1.5 1.5 1.6 1.5 1.5 1.4 1.6 1.7 1.4 1.6 1.4 1.6 1.6 1.6 1.6 1.7 1.6 1.7 1.8 1.8 1.7 2.0 1.7 1.4 1.4 1.4 1.3 1.1 1.1 1.1 1.1 0.9 0.9 0.7 22.4 22.5 27.3 26.4 26.0 26.4 24.1 20.4 23.0 23.0 20.9 22.3 23.7 23.0 23.5 25.6 26.3 24.1 24.8 24.5 2.1 1.8 2.1 1.9 2.0 1.8 1.9 1.6 1.6 1.7 1.5 1.5 1.5 1.7 1.5 1.6 1.6 1.7 1.7 1.5 1.5 1.5 1.6 1.6 1.6 1.5 1.8 1.6 1.5 1.6 1.7 1.6 1.8 1.5 1.6 1.6 1.7 1.7 1.5 1.7 1.5 1.7 1.6 1.3 1.5 1.3 1.4 1.4 1.3 1.5 1.4 1.5 1.7 1.5 22.4 29.2 24.0 27.2 24.7 29.5 26.6 24.1 24.5 25.8 29.2 30.3 27.4 25.0 27.9 27.5 28.9 27.8 27.0 1.8 1.9 1.8 1.9 1.8 1.7 1.8 1.9 1.6 1.6 1.7 1.6 1.5 1.7 1.7 1.6 1.6 1.6 1.7 1.6 1.6 1.7 1.8 1.8 1.8 1.7 1.6 1.7 1.7 1.7 1.8 1.6 1.6 1.6 1.6 1.7 1.7 1.7 1.7 1.7 1.8 1.7 1.6 1.5 1.4 1.5 1.5 1.5 1.6 1.4 1.7 1.7 1.6 1.5 1.5 26.8 28.6 29.7 33.6 25.4 24.4 23.8 24.5 25.4 24.7 25.4 24.3 25.6 27.1 26.8 24.3 25.7 24.3 1.8 1.7 1.6 1.7 1.5 1.6 1.6 1.6 1.4 1.5 1.3 1.5 1.5 1.7 1.5 1.7 1.5 1.4 1.6 1.8 1.7 1.7 1.6 1.6 1.7 1.6 1.6 1.6 1.7 1.6 1.9 1.6 1.6 1.5 1.5 1.5 1.6 1.6 1.7 1.7 1.7 1.7 1.5 1.2 1.5 1.3 1.4 1.5 1.4 1.4 1.3 1.5 1.5 1.3 1.3 1.3 52.4 54.1 59.1 48.2 41.6 38.9 37.1 36.6 36.0 33.5 34.7 39.7 40.6 39.9 42.4 38.3 41.7 1.7 1.7 1.7 1.6 1.6 1.6 1.6 1.4 1.5 1.5 1.6 1.6 1.6 1.8 1.5 1.7 1.5 1.4 1.5 1.7 1.6 1.6 1.5 1.6 1.7 1.6 1.5 1.6 1.6 1.6 1.7 1.5 1.8 1.5 1.6 1.4 1.6 1.7 1.6 1.7 1.7 1.7 1.6 1.3 1.4 1.4 1.4 1.4 1.5 1.3 1.3 1.5 1.4 1.4 1.5 1.3 0.7 53.5 57.5 45.2 36.6 35.8 38.7 33.8 35.1 29.2 28.4 31.9 35.6 35.2 35.8 34.3 39.1 1.9 2.1 1.8 1.8 1.7 1.7 1.8 1.7 1.4 1.6 1.5 1.5 1.6 1.7 1.5 1.7 1.6 1.5 1.7 1.7 1.7 1.6 1.6 1.6 1.7 1.6 1.6 1.6 1.7 1.7 1.6 1.5 1.6 1.6 1.6 1.4 1.7 1.7 1.6 1.7 1.8 1.7 1.5 1.2 1.4 1.4 1.5 1.5 1.4 1.4 1.3 1.5 1.5 1.4 1.3 1.2 0.6 0.6 58.4 45.6 34.4 36.5 36.5 33.5 33.2 31.4 32.8 33.7 38.1 36.7 39.1 36.1 39.5 1.8 1.9 1.7 1.8 1.8 1.6 1.6 1.6 1.5 1.5 1.6 1.6 1.6 1.7 1.6 1.6 1.5 1.5 1.5 1.7 1.8 1.6 1.5 1.6 1.7 1.6 1.5 1.5 1.6 1.6 1.8 1.5 1.7 1.6 1.6 1.5 1.7 1.6 1.7 1.7 1.7 1.6 1.5 1.3 1.5 1.3 1.4 1.3 1.5 1.3 1.2 1.5 1.5 1.4 1.4 1.1 0.5 0.6 0.5 45.2 36.2 35.8 38.3 32.7 32.8 29.5 28.4 33.0 35.9 36.3 36.2 33.9 36.5 1.9 1.8 1.7 1.9 1.8 1.5 1.5 1.6 1.6 1.6 1.6 1.6 1.6 1.7 1.6 1.6 1.5 1.6 1.6 1.6 1.6 1.6 1.6 1.4 1.7 1.6 1.6 1.6 1.6 1.7 1.9 1.6 1.6 1.5 1.5 1.6 1.7 1.7 1.7 1.7 1.6 1.7 1.7 1.2 1.6 1.5 1.4 1.5 1.5 1.4 1.3 1.5 1.6 1.5 1.2 1.4 0.7 0.8 0.8 0.8 47.8 42.1 39.6 35.6 35.9 35.2 36.4 42.7 42.5 41.7 43.1 42.4 46.1 1.8 1.8 1.8 1.7 1.7 1.9 1.7 1.8 1.7 1.8 1.7 1.5 1.6 1.8 1.5 1.5 1.4 1.5 1.9 1.8 1.7 1.7 1.8 1.8 1.9 1.7 1.9 1.8 1.8 1.8 2.0 1.8 1.9 1.7 1.8 1.8 1.8 1.7 1.8 1.8 1.8 1.9 1.5 1.4 1.5 1.5 1.5 1.6 1.5 1.5 1.4 1.5 1.5 1.6 1.3 1.4 0.8 1.0 1.0 1.0 0.7 62.1 57.8 37.9 39.2 38.6 39.7 43.0 46.7 44.6 47.8 43.5 47.5 1.8 1.9 1.8 1.7 1.9 1.7 1.7 1.6 1.6 1.6 1.6 1.5 1.5 1.7 1.7 1.5 1.4 1.5 1.8 1.7 1.7 1.5 1.8 1.7 1.9 1.6 1.8 1.7 1.8 1.7 1.9 1.8 1.7 1.7 1.7 1.8 1.8 1.8 1.7 1.8 1.7 1.8 1.7 1.4 1.7 1.5 1.6 1.6 1.6 1.4 1.4 1.6 1.3 1.5 1.4 1.4 0.9 1.0 1.0 1.0 0.8 0.5 64.4 35.7 39.1 37.7 38.0 43.8 45.3 44.6 44.3 41.1 44.3 1.8 2.0 1.9 1.7 1.7 1.7 1.7 1.6 1.7 1.6 1.9 1.6 1.6 1.9 1.7 1.5 1.5 1.4 1.7 1.8 1.6 1.7 1.8 1.5 1.8 1.8 1.9 1.7 1.8 1.7 1.8 1.7 1.8 1.6 1.8 1.6 1.7 1.7 1.7 1.8 1.7 1.8 1.6 1.4 1.7 1.4 1.6 1.4 1.6 1.4 1.5 1.5 1.6 1.5 1.4 1.4 0.9 0.9 1.0 0.9 0.9 0.5 0.5 32.5 37.0 34.4 36.6 41.0 38.8 41.9 40.4 37.6 40.0 1.8 2.1 2.0 1.9 1.9 2.0 1.8 1.6 1.5 1.6 1.5 1.6 1.6 1.7 1.6 1.6 1.6 1.6 1.9 1.7 1.7 1.6 1.8 1.6 1.9 1.8 1.9 1.6 1.7 1.8 1.9 1.8 1.9 1.7 1.7 1.6 1.8 1.8 1.8 1.8 1.9 1.7 1.8 1.4 1.8 1.7 1.7 1.6 1.5 1.5 1.3 1.5 1.5 1.6 1.4 1.4 1.0 1.1 1.1 1.1 1.1 1.0 1.0 1.1 63.6 35.6 38.9 39.6 41.2 39.1 44.4 40.4 43.3 1.8 1.9 1.9 1.8 2.0 1.9 1.9 1.7 1.6 1.6 1.7 1.6 1.7 1.6 1.7 1.6 1.6 1.5 1.6 1.6 1.7 1.7 1.9 1.5 2.0 1.6 1.8 1.7 1.7 1.7 1.8 1.8 1.8 1.7 1.7 1.7 1.8 2.0 1.8 1.8 1.8 1.8 1.7 1.4 1.7 1.5 1.5 1.5 1.5 1.5 1.4 1.5 1.4 1.5 1.2 1.4 1.0 1.1 1.1 1.1 1.0 0.9 0.9 1.0 0.4 43.0 43.0 42.5 45.2 40.9 46.1 44.5 47.2 1.8 2.0 1.7 2.0 2.1 1.8 1.9 1.7 1.6 1.7 1.7 1.6 1.6 1.7 1.7 1.7 1.7 1.7 1.6 1.6 1.7 1.7 1.9 1.6 1.8 1.7 1.8 1.7 1.8 1.7 1.8 1.7 1.9 1.7 1.6 1.7 1.7 1.8 2.0 1.7 1.7 1.8 1.8 1.3 1.6 1.6 1.5 1.7 1.7 1.5 1.5 1.6 1.5 1.5 1.2 1.4 1.1 1.3 1.2 1.2 1.1 1.0 1.0 1.1 1.0 0.9 60.4 43.9 45.2 43.4 46.1 41.5 43.9 1.8 2.0 1.7 1.9 2.1 1.9 1.9 1.7 1.7 1.9 1.6 1.6 1.6 1.8 1.8 1.5 1.6 1.6 1.7 1.6 1.5 1.7 1.6 1.6 1.5 1.5 1.7 1.5 1.9 1.7 1.7 1.8 1.8 1.6 1.6 1.5 1.7 1.7 1.8 1.7 1.7 1.7 1.8 1.4 1.7 1.7 1.7 1.7 1.4 1.6 1.5 1.4 1.5 1.5 1.3 1.4 1.1 1.3 1.1 1.3 1.0 0.9 0.9 1.0 0.9 0.9 0.5 42.7 46.8 44.2 42.2 39.5 41.8 1.8 2.0 1.9 1.8 1.8 1.8 1.8 1.6 1.6 1.6 1.5 1.5 1.6 1.6 1.5 1.6 1.6 1.7 1.8 1.6 1.6 1.6 1.8 1.5 1.7 1.7 1.7 1.8 1.7 1.8 2.0 1.8 1.7 1.5 1.6 1.6 1.6 1.7 1.6 1.7 1.7 1.7 1.6 1.5 1.7 1.7 1.4 1.6 1.5 1.5 1.4 1.5 1.5 1.5 1.4 1.4 0.9 1.1 1.1 1.1 0.8 0.8 0.8 0.8 0.9 0.8 0.8 0.8 69.9 67.7 53.4 48.8 55.6 1.8 1.8 1.7 1.8 1.7 1.8 1.7 1.6 1.6 1.7 1.4 1.5 1.5 1.6 1.5 1.5 1.5 1.6 1.8 1.6 1.6 1.6 1.7 1.6 1.8 1.6 1.7 1.7 1.7 1.6 1.9 1.6 1.8 1.5 1.6 1.5 1.7 1.8 1.6 1.7 1.6 1.6 1.6 1.4 1.6 1.6 1.4 1.5 1.4 1.4 1.3 1.4 1.4 1.4 1.3 1.4 0.9 1.0 0.9 1.0 0.9 0.7 0.7 0.9 0.9 0.8 0.8 0.7 0.4 77.0 56.1 52.5 57.2 1.7 1.8 1.8 1.8 1.8 1.7 1.7 1.5 1.6 1.6 1.5 1.5 1.6 1.6 1.6 1.5 1.5 1.6 1.8 1.6 1.6 1.5 1.7 1.6 1.8 1.7 1.7 1.7 1.7 1.7 1.8 1.6 1.6 1.5 1.6 1.5 1.7 1.7 1.6 1.7 1.6 1.6 1.7 1.4 1.6 1.6 1.5 1.5 1.5 1.5 1.4 1.4 1.4 1.3 1.3 1.4 0.9 1.0 1.0 1.0 0.9 0.8 0.7 0.8 0.9 0.9 0.8 0.8 0.4 0.3 55.4 49.3 56.8 1.9 2.0 1.8 1.8 1.9 1.8 1.8 1.5 1.5 1.6 1.5 1.4 1.5 1.6 1.5 1.5 1.6 1.6 1.8 1.5 1.6 1.5 1.6 1.6 1.7 1.6 1.7 1.8 1.7 1.7 1.8 1.7 1.6 1.5 1.6 1.5 1.6 1.6 1.6 1.6 1.5 1.6 1.8 1.3 1.7 1.6 1.5 1.5 1.4 1.4 1.4 1.5 1.5 1.5 1.3 1.4 0.9 1.1 1.0 1.0 0.9 0.7 0.8 0.9 0.8 0.8 0.8 0.9 0.6 0.6 0.6 62.4 75.8 1.9 1.9 1.7 1.8 1.8 1.9 1.9 1.6 1.5 1.7 1.6 1.5 1.7 1.7 1.6 1.6 1.4 1.5 1.6 1.5 1.6 1.6 1.7 1.6 1.7 1.6 1.7 1.8 1.8 1.6 1.8 1.6 1.6 1.6 1.5 1.5 1.7 1.7 1.7 1.7 1.6 1.7 1.8 1.3 1.6 1.6 1.5 1.5 1.5 1.5 1.4 1.5 1.4 1.4 1.3 1.4 1.0 1.1 1.0 1.1 0.9 0.8 0.9 0.9 0.9 0.8 0.9 0.9 0.7 0.6 0.7 0.5 67.2 1.8 1.8 1.8 1.7 1.8 1.7 1.8 1.6 1.6 1.7 1.6 1.4 1.7 1.7 1.5 1.5 1.4 1.4 1.8 1.5 1.7 1.6 1.7 1.5 1.6 1.8 1.7 1.8 1.8 1.8 1.9 1.8 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.6 1.8 1.3 1.7 1.7 1.4 1.4 1.4 1.4 1.4 1.5 1.4 1.4 1.3 1.4 0.9 1.0 0.9 1.0 0.8 0.7 0.8 0.9 0.8 0.8 0.8 0.9 0.6 0.5 0.5 0.3 0.4 Supplementary Figure 4: Maximum-likelihood phylogeny of the capsid protein sequences of viruses in the family Marnaviridae. Branch colours denote virus genera: Marnavirus (blue), Labyrnavirus (red), Locarnavirus (purple), Kusarnavirus (yellow), Bacillarnavirus (pink), Sogarnavirus (green) and Salisharnavirus (orange). SH-like branch support values are shown at the nodes when >0.70 and the maximum-likelihood scale bars indicate average residue substitutions per site.

BC-4 Supplementary Figure 5: Intra-genera relationships for Marnaviridae and select representative genera of the families Dicistroviridae, Picornaviridae and Potyviridae. The number of viruses is depicted as black circles while d are represented as bars: capsid (grey lines) and RdRP (solid grey) and polyproteins (white).

70 mean capsid diversity mean RdRp diversity mean polyprotein diversity number of viruses 120

60 98 100

50 80

40 60 30

Diversity (%) Diversity 40

20 of viruses Number

20 10 10 6 6 4 3 4 3 1 1 1 0 0

Potyvirus Marnavirus Aparavirus EnterovirusCardiovirus LabyrnavirusLocarnavirusKusarnavirus Sogarnavirus BacillarnavirusSalisharnavirus Supplementary Figure 6: Comparative RdRp and capsid protein sequence maximum-likelihood phylogenies of all the members of the family Marnaviridae. SH-like branch support values are shown at nodes when >0.70. Maximum-likelihood scale bars indicate average residue substitutions per site. Branches are coloured based on virus genera: Pairwise amino-acid comparisons of Marnaviridae RdRp sequences. Jukes-Cantor distances and Marnavirus (blue), Labyrnavirus (red), Locarnavirus (purple), Kusarnavirus (yellow), Bacillarnavirus (pink), Salisharnavirus (orange), Sogarnavirus (green) and Dicistrovirus (Cricket paralysis virus) (white).

RdRp Capsid

Tree scale: 0.1 NP 647481 Cricket paralysis virus Tree scale: 1 1 NP 620562 Triatoma virus NP 066241 Acute bee paralysis virus Cricket paralysis virus APG78541 Wenzhou picorna-like virus 21 APG78554.1 Wenzhou picorna-like virus 17 1 APG78550 Wenzhou picorna-like virus 20 APG76693.1 Beihai picorna-like virus 50 0.994 1 0.942 ASG92536 N OV 064 APG78898.1 Beihai picorna-like virus 50 APG78509 Wenzhou picorna-like virus 11 ASG92526.1 N OV 001 0.954 APG77368 Shahe picorna-like virus 14 APG77476.1 Sanxia picorna-like virus 10 0.789 1 APG77395 Shahe picorna-like virus 14 APG76812.1 Beihai picorna-like virus 57 1 APG78394 Hubei picorna-like virus 6 APG76773.1 Beihai picorna-like virus 57 AAP97137 Heterosigma akashiwo RNA virus APG78551.1 Wenzhou picorna-like virus 20 0.931 ASG92540 Q sR OV 020 ASG92537.1 N OV 064 1 0.985 ASG92542 Q sR OV 041 APG78542.1 Wenzhou picorna-like virus 21 APG78557 Wenzhou picorna-like virus 13 APG78507.1 Wenzhou picorna-like virus 22 0.957 APG79001 Changjiang picorna-like virus 2 APG78497.1 Wenzhou picorna-like virus 23 1 0.86 APG76773 Beihai picorna-like virus 57 APG76772.1 Beihai picorna-like virus 64 0.857 1 1 APG76812 Beihai picorna-like virus 57 APG76816.1 Beihai picorna-like virus 64 1 0.85 ASG92530 N OV 137 Aurantiochytrium single-stranded RNA virus 01 0.812 APG78434 Hubei picorna-like virus 7 APG76889.1 Beihai picorna-like virus 63 0.958 APG78407 Hubei picorna-like virus 7 YP 009255231.1 Antarctic picorna-like virus 3 0.932 1 APG78424 Sanxia atyid shrimp virus 2 APG78921.1 Beihai picorna-like virus 59 0.951 APG77465 Sanxia atyid shrimp virus 2 APG76708.1 Beihai picorna-like virus 59 1 YP 009255231 Antarctic picorna-like virus 3 APG76696.1 Beihai picorna-like virus 61 APG77377 Shahe picorna-like virus 6 APG76690.1 Beihai picorna-like virus 62 0.847 1 APG77381 Shahe picorna-like virus 6 APG76685.1 Beihai picorna-like virus 60 0.774 APG78591 Wenzhou picorna-like virus 19 APG78560.1 Wenzhou picorna-like virus 24 0.859 0.997 APG78971 Beihai picorna-like virus 56 APG78395.1 Hubei picorna-like virus 6 0.975 0.805 APG76693 Beihai picorna-like virus 50 APG79001.1 Changjiang picorna-like virus 2 0.879 1 APG78898 Beihai picorna-like virus 50 APG78525.1 Wenzhou picorna-like virus 16 APG78507 Wenzhou picorna-like virus 22 APG78917.1 Beihai picorna-like virus 13 0.888 0.962 0.991 APG78497 Wenzhou picorna-like virus 23 APG78899.1 Beihai picorna-like virus 32 APG76708 Beihai picorna-like virus 59 0.969 APG76844.1 Beihai picorna-like virus 32 0.965 1 APG78921 Beihai picorna-like virus 59 APG78557.1 Wenzhou picorna-like virus 13 APG76888 Beihai picorna-like virus 63 APG78594.1 Wenzhou picorna-like virus 14 0.91 BAE47143 Aurantiochytrium ssRNA virus APG78912.1 Beihai picorna-like virus 46 0.995 APG76771 Beihai picorna-like virus 64 APG76707.1 Beihai picorna-like virus 58 1 APG76815 Beihai picorna-like virus 64 0.769 APG78971.1 Beihai picorna-like virus 56 0.913 APG76690 Beihai picorna-like virus 62 APG78592.1 Wenzhou picorna-like virus 19 0.967 0.953 APG76696 Beihai picorna-like virus 61 APG77382.1 Shahe picorna-like virus 6 APG76685 Beihai picorna-like virus 60 1 0.933 0.918 APG77378.1 Shahe picorna-like virus 6 0.976 APG78560 Wenzhou picorna-like virus 24 0.8 APG77368.1 Shahe picorna-like virus 14 APG77477 Sanxia picorna-like virus 7 APG77395.1 Shahe picorna-like virus 14 1 0.968 APG78553 Wenzhou picorna-like virus 17 APG76698.1 Beihai razor shell virus 2 APG78020 Beihai picorna-like virus 42 0.885 APG78904.1 Beihai razor shell virus 2 1 0.701 0.849 APG76706 Beihai picorna-like virus 58 APG78901.1 Beihai razor shell virus 1 1 0.894 APG77485 Sanxia picorna-like virus 8 0.929 APG76731.1 Beihai razor shell virus 1 1 APG79000 Changjiang picorna-like virus 1 APG76695.1 Beihai razor shell virus 1 1 0.999 0.901 APG78521 Wenzhou picorna-like virus 12 0.948 APG78993.1 Changjiang picorna-like virus 3 ASG92524 N OV 080 0.804 APG78593 Wenzhou picorna-like virus 14 APG78867.1 Beihai picorna-like virus 44 APG76734 Beihai picorna-like virus 43 Marine RNA virus JP-B 0.981 APG76909.1 Beihai picorna-like virus 43 1 APG76791 Beihai picorna-like virus 43 0.885 0.792 APG76908 Beihai picorna-like virus 43 APG78019.1 Beihai picorna-like virus 43 1 0.897 APG76831 Beihai picorna-like virus 43 APG76735.1 Beihai picorna-like virus 43 APG78018 Beihai picorna-like virus 43 APG76792.1 Beihai picorna-like virus 43 0.817 YP 001429583 Marine RNA virus JP-B APG76832.1 Beihai picorna-like virus 43 APG78025.1 Beihai picorna-like virus 47 0.994 0.910.833 APG78992 Changjiang picorna-like virus 3 APG78502 Wenzhou picorna-like virus 15 APG77499.1 Hubei leech virus 3 0.98 APG76697 Beihai razor shell virus 2 APG77485.1 Sanxia picorna-like virus 8 0.902 0.983 0.963 APG78903 Beihai razor shell virus 2 APG79000.1 Changjiang picorna-like virus 1 0.999 APG78900 Beihai razor shell virus 1 APG78503.1 Wenzhou picorna-like virus 15 0.889 APG76694 Beihai razor shell virus 1 APG78995.1 Changjiang picorna-like virus 4 0.913 0.795 0.852 APG76730 Beihai razor shell virus 1 APG78401.1 Hubei picorna-like virus 4 0.996 0.958 APG78866 Beihai picorna-like virus 44 APG78393.1 Hubei picorna-like virus 5 0.891 AHA44480 Marine RNA virus SF-3 APG78892.1 Beihai picorna-like virus 49 0.998 1 APG77365 Shahe picorna-like virus 4 APG77495.1 Hubei leech virus 4 0.998 APG77387 Shahe picorna-like virus 4 APG79017.1 Hubei leech virus 4 1 0.9 0.891 APG78911 Beihai picorna-like virus 46 APG77384.1 Shahe picorna-like virus 5 1 APG78916 Beihai picorna-like virus 45 APG78505.1 Wenzhou channeled applesnail virus 1 1 0.883 0.937 APG77498 Hubei leech virus 3 APG78522.1 Wenzhou picorna-like virus 12 0.88 0.936 APG78398 Hubei picorna-like virus 2 Marine RNA virus SF-2 AFM44930 Marine RNA virus SF-1 APG78399.1 Hubei picorna-like virus 2 1 APG78402 Ubei picorna-like virus 3 Marine RNA virus SF-1 0.8880.926 APG78525 Wenzhou picorna-like virus 16 0.995 0.871 APG78403.1 Ubei picorna-like virus 3 APG78024 Beihai picorna-like virus 47 APG78918.1 Beihai picorna-like virus 48 0.72 APG77478 Sanxia picorna-like virus 9 APG77479.1 Sanxia picorna-like virus 9 1 1 APG78918 Beihai picorna-like virus 48 Marine RNA virus SF-3 0.741 0.743 AGZ83339 Marine RNA virus SF-2 APG77365.1 Shahe picorna-like virus 4 1 APG77383 Shahe picorna-like virus 5 APG77387.1 Shahe picorna-like virus 4 1 0.918 1 APG78504 Wenzhou channeled applesnail virus 1 APG78021.1 Beihai picorna-like virus 42 0.938 APG78400 Hubei picorna-like virus 4 ASG92525.1 N OV 080 0.972 APG78392 Hubei picorna-like virus 5 Rhizosolenia setigera RNA virus 0.832 APG78994 Changjiang picorna-like virus 4 APG78941.1 Beihai picorna-like virus 28 0.9890.925 0.865 APG78891 Beihai picorna-like virus 49 APG78983.1 Beihai picorna-like virus 26 0.996 APG77494 Hubei leech virus 4 APG78934.1 Beihai picorna-like virus 24 0.944 1 APG79016 Hubei leech virus 4 Chaetoceros socialis f radians RNA virus 0.971 APG76853 Beihai picorna-like virus 30 Chaetoceros tenuissimus RNA virus 01 0.793 APG78606 Beihai paphia shell virus 2 1 APG76848.1 Beihai picorna-like virus 27 0.999 APG78963 2 Beihai paphia shell virus 2 0.924 APG77487.1 Sanxia picorna-like virus 5 APG77473 Sanxia picorna-like virus 6 ASG92531.1 N OV 137 APG76803 Beihai picorna-like virus 36 APG77398.1 Shahe picorna-like virus 1 APG76901 Beihai picorna-like virus 37 0.953 APG77481.1 Sanxia picorna-like virus 3 0.999 APG78914 Beihai picorna-like virus 38 0.973 APG77468.1 Sanxia picorna-like virus 4 1 0.707 0.832 APG78578 Wenzhou picorna-like virus 53 APG77470.1 Sanxia picorna-like virus 1 0.881 APG76871 Beihai picorna-like virus 39 APG77467.1 Sanxia picorna-like virus 2 0.998 YP 009227212 Delisea pulchra RNA virus ASG92533.1 N OV 013 APG76861 Beihai sipunculid worm virus 3 0.999 APG76862.1 Beihai sipunculid worm virus 3 0.717 0.993 APG78922 Beihai sipunculid worm virus 3 APG77929.1 Beihai picorna-like virus 34 1 APG78555 Wenzhou picorna-like virus 9 0.937 APG76846.1 Beihai picorna-like virus 5 0.958 APG76858 Beihai sipunculid worm virus 2 0.998 APG76828.1 Beihai sipunculid worm virus 1 0.803 APG78919 Beihai picorna-like virus 31 APG76786.1 Beihai picorna-like virus 19 0.999 0.979 APG78585 Beihai mollusks virus 1 0.94 ASG92535.1 N OV 003 0.995 APG76840 Beihai mollusks virus 1 APG78876 Beihai mollusks virus 1 YP 009227213.1 Delisea pulchra RNA virus APG78556.1 Wenzhou picorna-like virus 9 0.997 APG76764 Beihai mollusks virus 1 APG78586.1 Beihai mollusks virus 1 0.99 0.996 APG78610 Beihai mollusks virus 1 APG78936 Beihai mollusks virus 1 APG78970.1 Beihai mollusks virus 1 1 APG78969 Beihai mollusks virus 1 APG78611.1 Beihai mollusks virus 1 0.991 YP 009047193 Asterionellopsis glacialis RNA virus APG78877.1 Beihai mollusks virus 1 APG78972 Beihai picorna-like virus 33 APG78937.1 Beihai mollusks virus 1 0.999 0.99 APG78952 Beihai mollusks virus 2 APG76841.1 Beihai mollusks virus 1 0.997 0.993 APG78581 Beihai mollusks virus 2 APG76765.1 Beihai mollusks virus 1 0.715 0.992 APG78924 Beihai mollusks virus 2 APG76857.1 3 Beihai picorna-like virus 7 APG76842 Beihai sipunculid worm virus 4 APG78910.1 Beihai picorna-like virus 6 1 0.989 0.999 0.731 APG76714 Beihai sipunculid worm virus 4 APG76838.1 Beihai picorna-like virus 6 0.727 0.837 APG78907 Beihai sipunculid worm virus 4 APG76737.1 Beihai picorna-like virus 9 0.98 APG76844 Beihai picorna-like virus 32 APG76678.1 Wenzhou picorna-like virus 52 0.706 0.932 0.996 APG78899 Beihai picorna-like virus 32 APG76852.1 Beihai picorna-like virus 8 0.973 APG78531 Wenzhou picorna-like virus 10 APG78955.1 Beihai picorna-like virus 11 0.972 1 1 APG78583 Wenzhou picorna-like virus 10 APG78573.1 Wenzhou picorna-like virus 1 0.99 APG77928 Beihai picorna-like virus 34 Marine RNA virus BC-2 0.775 0.923 APG78896 Beihai picorna-like virus 35 Marine RNA virus PAL156 0.708 0.982 1 APG78604 Beihai picorna-like virus 35 Marine RNA virus BC-3 APG77936 Beihai picorna-like virus 35 APG78920.1 Beihai picorna-like virus 31 APG78014 Beihai picorna-like virus 35 APG76859.1 Beihai sipunculid worm virus 2 1 APG76890 Beihai picorna-like virus 35 APG78923.1 Beihai sipunculid worm virus 3 0.885

APG76899 Beihai picorna-like virus 35 Asterionellopsis glacialis RNA virus 0.982 APG76877 Beihai picorna-like virus 35 APG78908.1 Beihai sipunculid worm virus 4 APG76823 Beihai picorna-like virus 35 APG76843.1 Beihai sipunculid worm virus 4 1 0.96 APG76752 Beihai picorna-like virus 35 APG76715.1 Beihai sipunculid worm virus 4 1 APG76710 Beihai picorna-like virus 35 APG78973.1 Beihai picorna-like virus 33 1 APG78978 Beihai picorna-like virus 35 APG78582.1 Beihai mollusks virus 2 0.991 APG78868 Beihai picorna-like virus 35 APG78925.1 Beihai mollusks virus 2 1 APG76783 Beihai picorna-like virus 35 0.995 APG78953.1 Beihai mollusks virus 2 0.971 ASG92532 N OV 013 APG78584.1 Wenzhou picorna-like virus 10 APG77399 Shahe picorna-like virus 3 APG78532.1 Wenzhou picorna-like virus 10 1 0.954 ASG92527 N OV 001 APG78979.1 Beihai picorna-like virus 35 ASG92534 N OV 003 APG78015.1 Beihai picorna-like virus 35 0.8541 APG76780 Beihai picorna-like virus 25 APG77937.1 Beihai picorna-like virus 35 0.972 0.71 1 APG78933 Beihai picorna-like virus 24 0.997 APG78605.1 Beihai picorna-like virus 35 APG78982 Beihai picorna-like virus 26 0.942 APG78869.1 Beihai picorna-like virus 35 0.89 BAG30951 Chaetoceros tenuissimus RNA virus 01 0.994 APG78897.1 Beihai picorna-like virus 35 1 APG76847 Beihai picorna-like virus 27 0.992 APG76784.1 Beihai picorna-like virus 35 0.946 APG78940 Beihai picorna-like virus 28 APG76711.1 Beihai picorna-like virus 35 0.914 0.945 BAE79742 Rhizosolenia setigera RNA virus 01 APG76753.1 Beihai picorna-like virus 35 YP 002647032 Chaetoceros socialis F. radians RNA virus 1 APG76878.1 Beihai picorna-like virus 35 APG77486 Sanxia picorna-like virus 5 APG76900.1 Beihai picorna-like virus 35 1 APG77469 Sanxia picorna-like virus 1 0.853 APG76824.1 Beihai picorna-like virus 35 0.998 APG77466 Sanxia picorna-like virus 2 APG76891.1 Beihai picorna-like virus 35 0.7950.859 APG77397 Shahe picorna-like virus 1 APG77465.1 Sanxia atyid shrimp virus 2 0.873 APG77468 Sanxia picorna-like virus 4 APG78424.1 Sanxia atyid shrimp virus 2 1 0.977 APG77480 Sanxia picorna-like virus 3 APG78434.1 Hubei picorna-like virus 7 1 0.818 APG78057 Wenzhou picorna-like virus 18 APG78407.1 Hubei picorna-like virus 7 1 ASG92522 N OV 011 0.896 APG76803.1 Beihai picorna-like virus 36 APG78574 Wenzhou picorna-like virus 4 0.965 0.777 APG76872.1 Beihai picorna-like virus 39 APG76869 Beihai picorna-like virus 18 0.985 APG78579.1 Wenzhou picorna-like virus 53 APG78028 Beihai sesarmid crab virus 1 0.959 APG76902.1 Beihai picorna-like virus 37 0.772 0.907 APG77496 Hubei leech virus 2 0.999 1 APG79027 Changjiang crawfish virus 1 APG78915.1 Beihai picorna-like virus 38 0.978 0.765 YP 009230118 Marine RNA virus PAL128 YP 009255230.1 Antarctic picorna-like virus 2 0.765 BC-1 Heterosigma akashiwo RNA virus APG78929 Beihai picorna-like virus 17 APG77400.1 Shahe picorna-like virus 3 0.79 APG76785 Beihai picorna-like virus 19 APG77477.1 Sanxia picorna-like virus 7 0.813 0.971 APG76827 Beihai sipunculid worm virus 1 Chaetoceros tenuissimus RNA virus type-II APG77476 Sanxia picorna-like virus 10 APG76717.1 Beihai picorna-like virus 2 0.886 0.91 ASG92549 Q sR OV 022 APG76796.1 Beihai picorna-like virus 2 0.833 APG78980 Beihai picorna-like virus 2 APG76757.1 Beihai picorna-like virus 2 1 0.73 APG77940 Beihai picorna-like virus 2 APG76826.1 Beihai picorna-like virus 2 0.816 1 APG76906 Beihai picorna-like virus 2 APG76907.1 Beihai picorna-like virus 2

APG78944 Beihai picorna-like virus 2 APG78945.1 Beihai picorna-like virus 2 0.958 1 0.983 APG76825 Beihai picorna-like virus 2 APG77941.1 Beihai picorna-like virus 2 0.986 1 APG76795 Beihai picorna-like virus 2 APG78981.1 Beihai picorna-like virus 2 APG76716 Beihai picorna-like virus 2 Chaetoceros sp. RNA virus 2 1 APG76756 Beihai picorna-like virus 2 APG78939.1 Beihai picorna-like virus 4 ASG92520 N OV 039 YP 001429582.1 Marine RNA virus JP-A 0.853 YP 009255232 Antarctic picorna-like virus 4 APG78568.1 Wenzhou picorna-like virus 50 0.858 1 YP 009230124 Marine RNA virus PAL473 APG78894.1 Beihai picorna-like virus 1 0.987 APG77366 Shahe picorna-like virus 2 APG78023.1 Beihai picorna-like virus 1 1 0.883 0.988 0.992 APG78948 Beihai picorna-like virus 21 APG76893.1 Beihai picorna-like virus 1 0.978 APG78558 Wenzhou picorna-like virus 32 APG76836.1 Beihai picorna-like virus 1 0.97 APG77933 Beihai picorna-like virus 22 APG76729.1 Beihai picorna-like virus 1 0.987 0.989 0.954 APG78926 Beihai octopus virus 1 APG76800.1 Beihai picorna-like virus 1 0.909 APG78055 Wenzhou picorna-like virus 7 ASG92549.1 Q sR OV 022 0.976 APG78565 Wenzhou picorna-like virus 6 0.981 APG77473.1 Sanxia picorna-like virus 6 0.997 ASG92547 Q sR OV 019 ASG92541.1 Q sR OV 020 0.878 0.99 BC-4 ASG92542.1 Q sR OV 041 0.981 APG78965 Beihai picorna-like virus 23 APG77367.1 Shahe picorna-like virus 2 0.988 APG78600 Beihai picorna-like virus 23 ASG92521.1 N OV 039 0.895 APG78946 Beihai picorna-like virus 23 APG78559.1 Wenzhou picorna-like virus 32 0.881 APG78476 Wenling picorna-like virus 1 APG78566.1 Wenzhou picorna-like virus 6 1 APG78576 Wenzhou gastropodes virus 1 APG76788.1 Beihai picorna-like virus 20 0.93 1 APG76787 Beihai picorna-like virus 20 APG78056.1 Wenzhou picorna-like virus 7 1 0.827 0.994 APG78570 Wenzhou picorna-like virus 5 APG78477.1 Wenling picorna-like virus 1 0.963 APG78883 Wenzhou picorna-like virus 5 1 APG78577.1 Wenzhou gastropodes virus 1 0.757 APG78942 Beihai paphia shell virus 1 0.991 APG78927.1 Beihai octopus virus 1 APG78967 Beihai paphia shell virus 1 0.973 APG78949.1 Beihai picorna-like virus 21 APG76675 Wenzhou picorna-like virus 2 0.947 APG78943.1 Beihai paphia shell virus 1 1 APG78545 Wenzhou picorna-like virus 2 APG78968.1 Beihai paphia shell virus 1 1 0.948 APG76845 Beihai picorna-like virus 5 APG78884.1 Wenzhou picorna-like virus 5 1 APG76855 Beihai picorna-like virus 7 0.717 APG78571.1 Wenzhou picorna-like virus 5 0.993 0.998 APG76837 Beihai picorna-like virus 6 Marine RNA virus BC-4 0.982 0.998 APG78909 Beihai picorna-like virus 6 ASG92548.1 Q sR OV 019 0.998 0.995 APG76736 Beihai picorna-like virus 9 APG78947.1 Beihai picorna-like virus 23 1 BC-2 APG78966.1 Beihai picorna-like virus 23 1 0.976 YP 009230120 Marine RNA virus PAL156 APG78601.1 Beihai picorna-like virus 23 0.756 APG76851 Beihai picorna-like virus 8 0.711 APG78029.1 Beihai sesarmid crab virus 1 BC-3 ASG92523.1 N OV 011 0.764 0.813 APG78954 Beihai picorna-like virus 11 APG77497.1 Hubei leech virus 2 0.88 APG78572 Wenzhou picorna-like virus 1 1 0.831 APG79028.1 Changjiang crawfish virus 1 APG76677 Wenzhou picorna-like virus 52 0.996 APG76870.1 Beihai picorna-like virus 18 YP 009255229 Antarctic picorna-like virus 2 Marine RNA virus PAL128 YP 009111336 Chaetoceros tenuissimus RNA virus type-II 0.884 BAK40203 Chaetoceros sp. RNA virus 2 APG76854.1 Beihai picorna-like virus 30 0.996 0.995 APG78938 Beihai picorna-like virus 4 APG78930.1 Beihai picorna-like virus 17 Marine RNA virus BC-1 0.929 0.853 0.874 APG78567 Wenzhou picorna-like virus 50 APG78575.1 Wenzhou picorna-like virus 4 0.715 0.954 YP 001429581 Marine RNA virus JP-A YP 009255233.1 Antarctic picorna-like virus 4 0.913 APG76892 Beihai picorna-like virus 1 APG76676.1 Wenzhou picorna-like virus 2 0.95 0.999 APG76835 Beihai picorna-like virus 1 1 0.802 APG76728 Beihai picorna-like virus 1 APG78546.1 Wenzhou picorna-like virus 2 APG78893 Beihai picorna-like virus 1 Marine RNA virus PAL473 APG78022 Beihai picorna-like virus 1 APG78916.1 Beihai picorna-like virus 45 0.917 APG76799 Beihai picorna-like virus 1 APG78607.1 Beihai paphia shell virus 2 1 APG78917 Beihai picorna-like virus 13 APG78964.1 Beihai paphia shell virus 2 APG76759.1 Beihai picorna-like virus 14 0.881 APG76758 Beihai picorna-like virus 14 0.752 0.999 APG78976 Beihai picorna-like virus 15 APG76834.1 Beihai picorna-like virus 15

0.987 APG78931 Beihai picorna-like virus 15 APG76798.1 Beihai picorna-like virus 15 0.806 1 APG78880 Beihai picorna-like virus 15 APG76725.1 Beihai picorna-like virus 15 0.89 0.903 APG78889 Beihai picorna-like virus 15 APG76742.1 Beihai picorna-like virus 15 0.964 APG78862 Beihai picorna-like virus 15 APG76887.1 Beihai picorna-like virus 15

APG78602 Beihai picorna-like virus 15 APG76913.1 Beihai picorna-like virus 15 0.976 APG77938 Beihai picorna-like virus 15 APG78932.1 Beihai picorna-like virus 15 0.961 APG76886 Beihai picorna-like virus 15 APG78881.1 Beihai picorna-like virus 15 1 APG76912 Beihai picorna-like virus 15 APG78863.1 Beihai picorna-like virus 15 APG76833 Beihai picorna-like virus 15 APG78890.1 Beihai picorna-like virus 15 0.72 APG76797 Beihai picorna-like virus 15 APG78603.1 Beihai picorna-like virus 15 APG76724 Beihai picorna-like virus 15 APG77939.1 Beihai picorna-like virus 15 APG78961 Beihai picorna-like virus 15 APG78962.1 Beihai picorna-like virus 15 1 APG76741 Beihai picorna-like virus 15 APG78977.1 Beihai picorna-like virus 15 1 Supplementary Figure7: Conserved RdRp motifs in members of the family Marnaviridae. The analysis involved the 20 viruses used to redefine the family (Table 1) as well as those falling with the 7 genera from the larger analysis (Supplementary Table 1). Domains I-VIII are as defined previously (Koonin and Dolja, 1993). Amino acid letters are coloured by their chemistry: polar uncharged (green), neutral hydrophilic (purple), basic positively charged (blue), acidic negatively charged (red) and hydrophobic non-polar (black).

I II III IV V VI VII VIII

All n=142n=9

Marnavirus

Labyrnavirus n=15n=34

Locarnavirus

Kusarnavirus n=14n=9

Bacillarnavirus

Salisharnavirus n=34n=27

Sogarnavirus

Polar Neutral Basic Acidic Hydrophobic Supplementary Figure 8: RdRp conserved alignment of the family Marnaviridae and type members of the order Picornavirales.

I II III IV V VI VII VIII

Triticum mosaic virus Wheat streak mosaic virus Sweet potato mild mottle virus Barley yellow mosaic virus Blackberry virus Y Potato virus Y Ryegrass mosaic virus Ampivirus A Kunsagivirus A Aquamavirus A Avihepatovirus A Avisivirus A Potamipivirus A Pasivirus A Limnipivirus A Parechovirus A Hepatovirus A Tremovirus A Harkavirus A Rabovirus A Sapelovirus A Enterovirus C Erbovirus A Teschovirus A Hunnivirus A Torchivirus A Mosavirus A Foot-and-mouth disease virus Cosavirus A Cardiovirus A Mischivirus A Senecavirus A Melegrivirus A Rosavirus A Cadicivirus A Salivirus A Aichivirus A Oscivirus A Sakobuvirus A Passerivirus A Sicinivirus A Gallivirus A Infectious flacherie virus Cricket paralysis virus Triatoma virus Acute bee paralysis virus Parsnip yellow fleck virus Tomato torrado virus Satsuma dwarf virus Cherry rasp leaf virus Tobacco ringspot virus Broad bean wilt virus 1 Rice tungro spherical virus Cowpea mosaic virus Heterosigma akashiwo RNA virus Aurantiochytrium ssRNA virus JP-B SF-2 SF-1 SF-3 Asterionellopsis glacialis RNA virus Chaetoceros tenuissimus RNA virus Rhizosolenia setigera RNA virus Chaetoceros socialis f. radians RNA virus 01 BC-4 PAL473 BC-1 PAL128 BC-2 PAL156 BC03 JP-A Chaetoceros tenuissimus RNA virus type-II Chaetoceros sp. RNA virus 2