Supplemental Data Cell Host & Microbe, Volume 5

Drosophila Intestinal Response to Bacterial Infection: Activation of Host Defense and Stem Cell Proliferation Nicolas Buchon, Nichole A. Broderick, Mickael Poidevin, Sylvain Pradervand, and Bruno Lemaitre

Supplemental Results Ecc15 is a strong inducer of the Imd pathway in the gut Drosophila ingestion of Ecc15 activates the Imd pathway in the adult gut (Ryu et al., 2006; Ryu et al., 2008; Zaidman-Remy et al., 2006). To further investigate the nature of this activation, we characterized the impact of Ecc15 on the gut transcriptional Imd response as compared to ingestion of other bacteria. Ingestion of Ecc15 increased the expression profile of three Imd target genes, Diptericin (Dpt), Attacin (Att) and PGRP-LB, in guts of wild-type but not Rel deficient flies (Figure S1A). However, Dpt expression was not induced when flies ingested either the Gram-positive bacterium Micrococcus luteus or the fungus Candida albicans. In addition, Dpt transcript levels increased following ingestion of monomeric (TCT) and polymeric peptidoglycan derived from Gram-negative, but not Gram-positive bacteria (Figure S1B). These results are in agreement with previous work showing that Dpt is induced in the gut by DAP-type peptidoglycan from Gram-negative bacteria (Zaidman-Remy et al., 2006). Ecc15 was also the best inducer of PGRP-LB although the expression of this amidase PGRP was also up-regulated by ingestion of M. luteus and C. albicans (Figure S1B). Moreover, ingestion of Ecc15 activates expression of the Dpt and PGRP-LB genes to levels similar to those obtained by artificial activation of the Imd pathway through over-expression of imd using a heat-shock driver (genotype: hs-Gal4; UAS-imd; Figure S1C). Together, these results show that Ecc15 is a potent inducer of the gut Imd response, as this bacterium induced Dpt expression to nearly the same level as artificial activation of the Imd pathway.

Ingestion of Ecc15 elicits a complex gut immune response To characterize Dpt expression along the digestive tract in response to Ecc15 infection, we performed LacZ staining of guts dissected from Ecc15 infected wild-type and PGRP-LC deficient flies carrying a Dpt-lacZ reporter gene. The Dpt-lacZ reporter transgene was expressed with a stereotyped expression pattern along the gut of infected wild-type but not

1 PGRP-LCE12 flies (Figure S2A, see schematic representation in S2B). A discrete ring of expression was observed in the anterior cardia (a sphincter at the foregut-midgut junction), followed by three strong Dpt-lacZ expression domains (R1, R2 and R3) separated by stretches with no expression. Expression in the posterior-most region of the gut was variable as reflected in the patchy expression of lacZ in a dispersed subset of cells (R4 Figures S2A and B). A similar stereotyped pattern was observed when Dpt expression was monitored with a Dpt-GFP reporter gene or by in situ hybridization (data not shown, Figure S2C) indicating that the stereotyped lacZ pattern accurately reflects Dpt expression and is not due to the activity of the endogenous Drosophila ß-galactosidase gene Gal (* in Figure S2A). Moreover, a similar pattern of Dpt-lacZ expression was observed in the gut upon artificial activation of the Imd pathway in Dpt-lacZ; hs-Gal4; UAS-imd flies (data not shown). The results obtained with these flies indicate that the patterned expression of Dpt along the gut represents the full range of expression of the antibacterial peptide gene and is not due to a modulation of Imd pathway activation by the distribution of bacteria along the gut. This restricted pattern of Dpt expression may reflect patterned intracellular Imd pathway activity, which seems to be functional only in a subset of gut cells. Alternatively, there may be an additional level of regulation that restricts Imd-dependent Dpt expression to specific domains of the gut. To distinguish between these two hypotheses, we determined by immuno-staining the expression of another Imd pathway target, PGRP-LB, in control and Ecc15 infected flies. In contrast to Dpt, there was no patterning of PGRP-LB expression (Figure S2D), indicating that the Imd pathway is activated all along the gut and that additional regulatory elements likely block Dpt expression in some gut cells. This is supported by our observation that the PGRP-LC gene was also expressed all along the gut (data not shown) and by a recent study showing that the homeobox protein Caudal represses Dpt in the distal part of the adult gut (Ryu et al. 2008). Together, these results reveal that the gut is a complex and compartmentalized organ with distinct immunoreactive domains. They show that Ecc15 is a potent inducer of the gut Imd response following ingestion, as this bacterium induced Dpt expression with the same pattern as artificial activation of the Imd pathway.

2 Supplemental Experimental Procedures Fly stocks OregonR flies or flies carrying one copy of the NP1-Gal4 transgene were used as wild-type controls as previously described (Zaidman et al 2006). UAS-RNAi transgenic fly lines of dFADD (R1), PGRP-LC (R1), STAT (R2), were obtained from Ryu Ueda (Mishima, Japan). upd3-IR, upd3-Gal4, UAS-GFP; hopmsv1 and hopTum are described in (Agaisse et al., 2003). In the present study, we used adult flies carrying one copy of the UAS-RNAi construct combined with one copy of the NP1-Gal4 driver. The F1 progeny carrying both the UAS-RNAi and the Gal4 driver were transferred to 29°C at late pupal stage for optimal GAL4 and RNAi efficiency. The transgenic strains Dpt-lacZ, Dpt-GFP, UAS-imd and the mutant lines PGRP- LCE12, RelE20, Tl1-RXA, and Tlr632have been described elsewhere (Romeo and Lemaitre, 2008). A 10XSTAT92E-eGFP construct that contains 10 Stat92E binding sites driving expression of enhanced GFP were used to monitor JAK-STAT pathway signaling activity (Bach et al., 2007). The esg-Gal4; UAS-mCD8GFP line is described in (Micchelli and Perrimon, 2006) (Yagi and Hayashi, 1997). esg-Gal4; P[pTub-GAL80] is described in (Liu and Hou, 2008) pros-Gal4 line has been described in (Ohshiro et al., 2000). PucE69 is a P-lacZ (enhancer trap) element inserted in the phosphatase gene puc (Martin-Blanco et al., 1998). Drosophila stocks were maintained at 23°C using standard fly medium.

Clonal analysis We used the marked lineage system developed by Harrison and Perrimon to generate clones of lacZ expressing cells (Harrison and Perrimon, 1993; Ohlstein and Spradling, 2006). In this system, flippase mediated recombination fuses the !-tubulin promoter to the lacZ gene allowing transcription of the marker. In the absence of heat-shock, X15 flies carry two inactive tubulin promoter-lacZ (X-15-29 and X15-33). Upon heat-shock induction of flipase in dividing cells, these transgenes can recombine through the flipase-recombination target (FRT) region, generating an active lacZ transgene. Thereafter, the cell carrying the recombined lacZ transgene as well as its progeny will be marked by constitutive production of ß-galactosidase. The size of the marked clone is a direct measure of the division rate. Adults of the genotype yw, hs-FLP; X-15-33/X-15-29 were produced by standard crosses. To induce somatic recombination, adult flies were heat-shocked 5 days after eclosion at 37°C for 60 min during 3 days. No lacZ-positive cells could be found in the intestine in the absence of heat-shock. A day thereafter, flies were orally infected with Ecc15 or fed on sucrose. Guts

3 were dissected 72 hr after infection and stained with X-gal.

Bacterial strains and infection experiments E. carotovora carotovora 15 is a Gram-negative bacterium that induces a systemic immune response after oral infection in larvae but not in adults (Basset et al., 2000). For oral infection, flies were first incubated 2 hr at 29°C in an empty vial and then placed in a fly vial with food solution and maintained at 29°C. The food solution was obtained by mixing a pellet of an overnight culture of bacteria (OD 200), peptidoglycan (5 mg/ml) or TCT (tracheal cytotoxin) (0.046 mM) with a solution of 5% sucrose (50/50) and added to a filter disk that completely covered the surface of standard fly medium (Zaidman-Remy et al., 2006). Flies were dissected 4 and 16 hr after oral contact with infected food. Escherichia coli (E. coli, strain 1106), Micrococcus luteus (M. Luteus), Enterococcus Faecalis (E. faecalis), Candida albicans (C. albicans) are described in (Basset et al., 2000; Gottar et al., 2007).

Reverse transcription Quantitative PCR (RT-qPCR) RNA was extracted from 50 dissected guts using RNA TRIzol® (Invitrogen). Briefly, dissected guts were placed directly into a screw-top microcentrifuge tube containing 500 µl of TRIzol and glass beads. Samples were homogenized with a Precellys 24 (Bertin Technologies) and further extracted according to the manufacture’s instructions. RNA was quantified with a NanoDrop®ND-1000 and 1 µg of RNA was used as template for RT using SuperScript II (Invitrogen) according to manufacture’s instructions and as previously described (Zaidman-Remy et al., 2006). Quantitative PCR was performed using dsDNA dye SYBR Green I (Roche Diagnostics) and reactions were performed on a Lightcycler 2.0 (Roche). The amount of mRNA detected was normalized to control rp49 mRNA values. We used normalized data to quantify the relative levels of a given mRNA according to cycling threshold analysis ("Ct). For the Y-axis, we used the value "Ct Dipt/"Ct RP49 to allow comparison between the different experiments. Relative gene expression is a percentage expression of the ratio value obtained in WT infected guts from the same experiment. Primer pairs for Dpt, Att, PGRP-LB, Rel, IM3, Drs, DrsE, Socs36E, upd3, GstD8, hsp70, CG4367, CG8317, CG9080, CG6933, CG15282 can be provided upon request.

Live imaging and immunofluorescence For live imaging, guts were dissected in phosphate buffered saline (PBS) and immediately

4 mounted in the anti-fading agent Citifluor AF1 (Citifluor). Samples were observed for fluorescence with an axioplot imager Z1 (Zeiss), and an Axiocam mRM camera (Zeiss). For immunofluorescence, guts were dissected in PBS, fixed for 20 min in 0.1% Tween 20 PBS (PBT) with 4% paraformaldehyde, rinsed in PBT, and then incubated with primary antibodies (dilution 1/150 anti-LB, 1/1000 anti-PH3, 1/500 anti-cleaved Caspase-3 or 1/1000 anti-GFP) in PBT + 1% BSA. Anti-LB or anti-GFP was revealed by secondary anti-Mouse antibodies coupled with Alexa488 or Alexa594- (Invitrogen) and nuclei stained by DAPI (Sigma). Apoptosis was detected in dissected guts by acridine orange and TUNEL staining according to the manufacture’s instructions (Invitrogen and Roche, respectively). Briefly, dissected guts were placed into PBS. Acridine orange was added to a final concentration of 1 mg/ml for 3 min. After several washes in PBS, guts were mounted onto slides and observed using fluorescence microscopy. For in situ TUNEL labeling, guts were dissected and then fixed with PBS 4% paraformaldehyde for 30 min at room temperature. After fixation, the guts were rinsed three times with PBT. TUNEL labeling was carried out with the reagents and procedures supplied with the Roche In Situ Cell Death Detection Fluorescein labeling kit. The TUNEL labeling reaction was carried out for 1 h at 37ºC. The TUNEL labeled guts were washed with PBS for 1 hr and then DAPI was added and staining was monitored by fluorescence microscopy.

In Situ Hybridization Gut tissue from adult flies was fixed and hybridized with digoxigenin-UTP antisense RNA probes (Roche Diagnostics) using the method described for Drosophila embryos in (Jiang et al., 1991). The same oligos used for Dpt RT-qPCR were used to generate the matrix for Dpt probe synthesis.

ß-galactosidase reporter transgene activity staining Guts were dissected, fixed and stained according to (Romeo and Lemaitre, 2008). Images were aquired with a Leica MZ16F microscope and a Leica DFC 300 FX camera.

Analysis of whole genome mRNA expression by Affymetrix Droso2.0 chips RNA pools from 60 guts of 5 days old females were isolated, purified with RNA clean up purification kits (Macherey Nagel), and DNase treated. All RNA quantities were assessed by NanoDrop®ND-1000 spectrophotometer and the quality of RNA was controlled on Agilent 2100 bioanalyzer chips. For each sample, 1 #g of total RNA was amplified and labeled using

5 the GeneChip IVT labelling kit according to the protocol provided by the supplier. Affymetrix Drosophila Genome 2.0 arrays were hybridized with 30 #g of labeled cRNA, washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol EukGeWS2v5_450).

Data analysis Three independent repeats were performed for each time point and the gene expression profiles from challenged flies were normalized to the sucrose fed adults. All statistical analysis were performed using the free high-level interpreted statistical language R (R Core, 2004, http://www.R-project.org) and various Bioconductor packages (http://www.Bioconductor.org). Hybridization quality was assessed using Bioconductor “affy” and “affyPLM” packages (Bolstad et al., 2003; Gautier et al., 2004). Clustering analysis using all 18,952 Probe sets on the array revealed that biological repeats (triplicates) cluster together with the exception of the unchallenged wild-type where samples from the 2 time points cluster together (Figure S5). This shows that in wild-type unchallenged flies, samples are stable over time and that the within group variability is always lower than the between group variability. Normalized expression signals were calculated from Affymetrix CEL files using the RMA normalization method implemented in the “affy” package (Bolstad et al., 2003; Irizarry et al., 2003). Differential hybridized features were identified using Bioconductor package “limma” that implements linear models for microarray data (Smyth, 2004). In “limma”, P values are obtained from moderated t-statistics or F-statistics using Empirical Bayesian methods. P values are then adjusted for multiple testing with Benjamini and Hochberg’s method to control the false discovery rate (FDR) (Benjamini and Hochberg, 1995). Probe sets showing at least 2-fold change and a FDR < 0.01 were considered significant. RT-qPCR of independent gut extracts from control and Ecc15 orally infected flies confirmed the inducibility of 20 out of 20 tested genes, validating our dataset (indicated on Table 1). This microarray analysis likely missed some genes, as we did not monitor those regulated before 4 hr after ingestion of Ecc15 (ex. Puckered Figure S3). In addition, we cannot exclude that some genes may be modulated by the potential nutritive value of ingested bacteria, rather than by infection. The complete set of gut Ecc15 regulated genes as well as the complete underlying data are available at http://lemaitrelab.epfl.ch/ (folder “Resources”).

6 References Agaisse, H., Petersen, U.M., Boutros, M., Mathey-Prevot, B., and Perrimon, N. (2003). Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury. Dev Cell 5, 441-450. Bach, E.A., Ekas, L.A., Ayala-Camargo, A., Flaherty, M.S., Lee, H., Perrimon, N., and Baeg, G.H. (2007). GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo. Gene Expr Patterns 7, 323-331. Basset, A., Khush, R., Braun, A., Gardan, L., Boccard, F., Hoffmann, J., and Lemaitre, B. (2000). The phytopathogenic bacteria, Erwinia carotovora, infects Drosophila and activates an immune response. Proc Natl Acad Sci USA, 3376-3381. Benjamini, Y., and Hochberg, Y. (1995). Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B 57, 289-300. Bolstad, B., Irizarry , R., Astrand, M., and Speed, T. (2003). A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185-193. De Gregorio, E., Spellman, P.T., Rubin, G.M., and Lemaitre, B. (2001). Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays. Proc Natl Acad Sci U S A 98, 12590-12595. Gautier, L., Cope, L., Bolstad, B.M., and Iriyarry, R.A. (2004). affy—analysis of affymetrix genechip data at the probe level. Bioinformatic 20, 307–315. Gottar, M., Gobert, V., Matskevich, A., Reichhart, J.M., Wang, C., Butt, T.M., Belvin, M., Hoffmann, J.A., and Ferrandon, D. (2007). Dual detection of fungal infections in Drosophila by recognition of glucans and sensing of virulence factors. Cell 127:1425-37. Harrison, D.A., and Perrimon, N. (1993). Simple and efficient generation of marked clones in Drosophila. Curr Biol 3, 424-433. Irizarry, R.A., Hobbs, B., Collin, F., Beazer-Barclay, Y.D., Antonellis, K.J., Scherf, U., and Speed, T.P. (2003). Exploration, Normalization, and Summaries of High Density Oligonucleotide Array Probe Level Data. Biostatistic 4, 249-264. Jiang, J., Kosman, D., Ip, Y.T., and Levine, M. (1991). The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos. Genes Dev 5, 1881-1891. Liu, W., and Hou, S.X. (2008). Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells. Methods Mol Biol 450, 61-70. Martin-Blanco, E., Gampel, A., Ring, J., Virdee, K., Kirov, N., Tolkovsky, A.M., and Martinez-Arias, A. (1998). puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila. Genes Dev 12, 557-570. Micchelli, C.A., and Perrimon, N. (2006). Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature 439, 475-479. Ohlstein, B., and Spradling, A. (2006). The adult Drosophila posterior midgut is maintained by pluripotent stem cells. Nature 439, 470-474. Ohshiro, T., Yagami, T., Zhang, C., and Matsuzaki, F. (2000). Role of cortical tumour- suppressor proteins in asymmetric division of Drosophila neuroblast. Nature 408, 593-596. Romeo, Y., and Lemaitre, B. (2008). Drosophila immunity : methods for monitoring the activity of toll and imd signaling pathways. Methods Mol Biol 415, 379-394. Ryu, J.H., Ha, E.M., Oh, C.T., Seol, J.H., Brey, P.T., Jin, I., Lee, D.G., Kim, J., Lee, D., and Lee, W.J. (2006). An essential complementary role of NF-kappaB pathway to

7 microbicidal oxidants in Drosophila gut immunity. Embo J 25, 3693-3701. Ryu, J.H., Kim, S.H., Lee, H.Y., Bai, J.Y., Nam, Y.D., Bae, J.W., Lee, D.G., Shin, S.C., Ha, E.M., and Lee, W.J. (2008). Innate immune homeostasis by the homeobox gene caudal and commensal-gut mutualism in Drosophila. Science 319, 777-782. Smyth, G.K. (2004). Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol PMID: 16646809 Yagi, Y., and Hayashi, S. (1997). Role of the Drosophila EGF receptor in determination of the dorsoventral domains of escargot expression during primary neurogenesis. Genes Cells 2, 41-53. Zaidman-Remy, A., Herve, M., Poidevin, M., Pili-Floury, S., Kim, M.S., Blanot, D., Oh, B.H., Ueda, R., Mengin-Lecreulx, D., and Lemaitre, B. (2006). The Drosophila amidase PGRP-LB modulates the immune response to bacterial infection. Immunity 24, 463-473.

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Figure S3. Activation of the JNK Pathway in the Gut upon Ecc15 Infection Expression of lacZ in the pucE69 line was monitored to detect JNK activation in the guts of unchallenged (UC) or challenged flies 16 hr after ingestion of Ecc15. Arrow indicates site of induction of puc-lacZ, * indicates expression of endogenous ß-galactosidase.

Table S6

D Fold change compared to I time R Gene.Symbol CG number Molecular function G wild type uninfected guts Rel response Or 4h Or 16h Rel 4h Rel 16h

ANTIMICROBIAL RESPONSE Recognition PGRP-SD CG7496 PGRP + sustained + 32.6 11.8 -1.3 -1.8 PGRP-LF CG4437 PGRP + sustained + 6.3 2.6 -1 -1.4 TepIV CG10363 Thiol Ester Proteins (TEPs) sustained + 4.8 2.2 3.1 1.7 PGRP-SB1 CG9681 PGRP + sustained + 3.6 4.9 1 1.3 PGRP-SC1a/b CG8577 PGRP + sustained + 3 3.8 -14.1 -11.2 PGRP-LB CG14704 PGRP + sustained + 2.6 2.9 -2.2 -1.5 PGRP-SA CG11709 PGRP early + 2.5 1.4 2.1 -1.2 Idgf3 CG4559 Imaginal Disc Growth Factors (IDGF) early + 2.5 1.8 2 1.4 TepII CG7052 Thiol Ester Proteins (TEPs) early + 2.5 1.2 1.4 -1.2 Idgf1 CG4472 Imaginal Disc Growth Factors (IDGF) early + 2.1 1.2 2.3 1.1 CG13422 CG13422 GNBP-like late + 1.9 2.7 1.7 1.4 Antimicrobial activity AttD CG7629 Attacin + sustained + 77.6 43.7 -4.9 -6.2 AttA CG10146 Attacin + sustained + 22.1 15.2 -1.7 -1.7 AttA/B CG18372 Attacin + sustained + 9.3 7.1 -1.3 -1.5 Mtk CG8175 Metchnikowin + sustained + 7.4 29.2 -1 -1.6 dro2 CG32279 Drosomycin-like sustained 6.8 3.2 5.8 2.7 Def CG1385 Defensin + sustained + 6.7 23.7 -9.3 -5.1 dro3 CG32283 Drosomycin-like sustained 6.6 8 5.6 5.9 dro4 CG32282 Drosomycin-like early 3.6 1.7 2.8 1.2 Drs CG10810 Drosomycin early + 2.9 1.9 1.1 -2.2 DptB CG10794 Diptericin + sustained + 2.8 6.9 -1.3 -1.2 AttC CG4740 Attacin + sustained + 2.3 4.3 -1 -1.2 LysX CG9120 Lysosyme late 1.9 2.3 1.8 1.5 DIMs IM3 CG16844 Immune induced molecule + sustained 2.7 2.1 -2.9 -2.7 CG16836 CG16836 Immune induced molecule + early + 2.1 -1.1 -1 -1.6 IM1 CG18108 Immune induced molecule + late + 1.3 2.9 -1 -1 IM10 CG18279 Immune induced molecule + late + 1.3 2.2 1.1 -1.1 Inhibitors of proteases Spn4 CG9453 Serpin (neuroserpin) + sustained + 5.1 2.3 2.4 1.2 CG3604 CG3604 serine-type endopeptidase inhibitor activity-Kunitz + sustained + 4.2 3.1 1.9 1.1 CG16713 CG16713 endopeptidase inhibitor activity-Kunitz + sustained + 4.1 3 2 -1.2 CG16712 CG16712 endopeptidase inhibitor activity-Kunitz + sustained 3 3 1.5 -1.2 CG31704 CG31704 Proteinase inhibitor I1, Kazal + sustained 2.9 2.3 -1.2 -2.3 Misceallaneous Tsf1 CG6186 iron sequestration + sustained + 3.4 4.5 -1.6 1 SIGNALING PATHWAYS Imd pathway Pirk CG15678 downregulation of PGRP LC signaling + sustained + 19.8 10.4 1.5 1.6 Rel CG11992 transcription factor + early + 2.6 1.7 ND ND key CG16910 homologue of the ikkg + early 2.4 1.3 1.3 -1.2 Toll pathway spz CG6134 ligand of the Toll receptor late + 1.5 2.4 1.7 1.7 JNK pathway ALiX CG12876 putative positive regulator of JNK pathway early 2.6 1.3 2.4 1.3 Jra CG2275 transcription factor activity early 2.2 1.3 2 1 MAPK pathway Mkp3 CG14080 MAPKKK cascade, phosphatase activity early 2.4 1.5 2.7 1.5 p38c CG33338 MAPK early 2.1 1.6 2.4 1.6 JAK-STAT pathway Socs36E CG15154 regulation of jak stat pathway sustained 8.3 2.1 7.6 1.8 upd3 CG5963 ligand of Domeless sustained 4.6 2.2 5.1 2.7 dome CG14226 Receptor of the JAK-STAT pathway early 4.3 1.9 3.1 1.5 EGF pathway rho CG1004 serine-type peptidase + sustained 4.2 2.2 2.1 1.5 vn CG10491 epidermal growth factor receptor binding sustained 2.9 2.3 2.2 1.6 Krn CG32179 epidermal growth factor receptor binding early 2.5 1.5 2.8 1.8 rho-4 CG1697 serine-type peptidase sustained 2.4 2 2.5 1.7 edl CG15085 regulation of epidermal growth factor receptor signaling pathway early 2.4 1.6 2.3 1.3

Nrg CG1634 calcium ion binding early 2.3 1.6 1.8 1.8 argos CG4531 EGF-Receptor ligand + early 2.2 1.9 -1 -1.3 pnt CG17077 Transcription factor + late 1.6 2 -3.2 -2.3 Notch pathway fng CG10580 Notch pathway early 2.5 -1 1.9 -1 Su(dx) CG4244 notch signaling pathway, ubiquitin ligase activity early 2.1 1.5 1.3 1.3 Dl CG3619 notch signaling pathway + sustained 2 2.3 1.2 1.1 PDGF- and VEGF-related factor 1 Pvf1 CG7103 PDGF- and VEGF-related factor 1 sustained 3.3 2.4 2.5 1.6 Pvf2 CG13780 PDGF- and VEGF-related factor 2 early 2.1 1.3 1.8 1.7 Hedgehog signaling hh CG4637 hedgehog + early 2.8 1.9 -1.3 -1.7 Other signaling pathway components Ets21C CG2914 transcription factor activity sustained 6.2 2 5.6 1.6 CG33958 CG5719 guanylate cyclase activity + sustained 5.3 2 1 -1.3 Phk-3 CG9358 protein serine/threonine kinase, diacylglycerol binding early 4.7 1.4 4.6 1.2 CG6805 CG6805 Inositol polyphosphate related phosphatase early 4.3 1.9 2.3 1.2 CG5036 CG5036 regulator of G protein signaling activity early 3.5 1.7 2.3 1.6 CG3008 CG3008 protein kinase activity early 3.2 1.4 3.7 2.4 l(1)G0232 CG32697 protein tyrosine phosphatase activity early 3.1 1.5 2.2 1.3 Mad CG12399 TGF beta pathway early 2.9 1.3 1.8 -1.3 CG11318 CG11318 G protein coupled receptor activity early 2.2 1.5 2.3 -1 CG31158 CG31158 ARF guanyl-nucleotide exchange factor activity early 2.2 1.4 1.9 1.2 CG11968 CG11968 G-protein coupled receptor protein signaling pathway early 2.2 1.1 2.2 1.1 vap CG9209 Ras GTPase activator activity early 2.1 1.2 1.4 -1 Arf84F CG7435 G-protein coupled receptor protein signaling pathway early 2.1 1.6 2.2 1.4 mthl4 CG6536 G-protein coupled receptor protein signaling pathway early 2.1 1.6 1.6 1.1 Rho1 CG8416 GTPase activity early 2.1 1.5 1.9 1.3 RanGap CG9999 Ran GTPase activity sustained 2 2.2 1.9 2.4 STRESS RESPONSE / DETOXIFICATION Heat Shock Proteins (HSP) Hsp70Aa/b CG18743 Heat shock protein early 7.4 -1.6 9.3 1.8 Hsp70Ba/b/bb/c CG6489 Heat shock protein sustained + 3.3 -2 7 1.6 Hsp70Bc CG6489 Heat shock protein early + 3.1 -1.5 3.9 1 Hsp23 CG4463 Heat shock protein - sustained 3.1 -2.9 10.4 3 Hsc70-2 CG7756 Heat shock protein + early 2.8 1.4 1.2 1 Hsp83 CG1242 protein folding / centrosome early 2.4 1.3 2.4 1.2 Hsp26 CG4183 Heat shock protein - sustained 2.3 -3 4.9 1.9 Hsp27 CG4466 Heat shock protein early 2.2 1.5 2.5 2.5 Hsp68 CG5436 Heat shock protein - sustained 2.2 -3.5 4.6 1.6 Cytochrome P450 Cyp6d2 CG4373 cytochrome P450 sustained 7 4.6 7.3 8.4 Cyp305a1 CG8733 cytochrome P450 early 3.8 1.5 2.4 1.1 Cyp4p3 CG10843 cytochrome P450 + early + 3.6 -1.2 1.8 -2 Cyp4p1 CG10842 cytochrome P450 - early 3 1.9 9.4 3.8 Cyp6a18 CG13977 cytochrome P450 - early 2.6 -1.4 6.3 1.1 Glutathione S transferase activity (Delta cluster) GstD8 CG4421 Glutathione S transferase activity sustained 48.2 48.9 63.5 70.7 GstD5 CG12242 Glutathione S transferase activity - early 5.3 -1.3 22.6 6.2 GstD4 CG11512 Glutathione S transferase activity - early 2.6 1.9 43.7 22.3 GstD2 CG4181 Glutathione S transferase activity - sustained 2 -7 21.4 7.3 GstD7 CG4371 Glutathione S transferase activity - late 1.6 2.1 4.5 7.2 Oxydative stress response CG2065 CG2065 oxidoreductase activity early 11.2 1.8 10.3 5.1 CG12224 CG12224 oxidoreductase activity + early 6.2 1.5 1.2 -1.1 CG10638 CG10638 aldehyde reductase activity sustained 4.3 3 3.8 3.7 CG12766 CG12766 aldehyde reductase early + 3.8 1.5 3.1 2 CG10962 CG10962 oxidoreductase activity early 3.2 1.8 5.2 1.9 CG2064 CG2064 oxidoreductase activity early 3.1 1.8 3 2.6 Trx-2 CG31884 cell redox homeostasis early 2.1 1.2 1.5 1.2 CG8776 CG8776 carbon-monoxide oxygenase activity early 2 -1.3 2.2 -1.1 Ts CG3181 oxidoreductase activity late -1.1 2.4 -1 1.9 Turandots TotC CG31508 stress peptide sustained 2.7 2 3.6 3.8 TotM CG14027 stress peptide + early + 2.3 1.3 -1.1 1.4 TotA CG31509 stress peptide sustained 2 2 1.3 1.9 Miscellaneous Fst CG9434 cold response sustained + 18.3 4.9 17.2 6.2 CG11897 CG11897 multidrug transporter activity early 3.1 1.3 2.6 1.2 CG7806 CG7806 xenobiotic-transporting ATPase activity early 2.1 1.2 2.3 1.3 Peritrophic matrix constituant / chitin metabolism CG32302 CG32302 chitin binding and chitin metabolic process + sustained 13.4 13.6 1.9 3.2 CG32284 CG32284 chitin binding and chitin metabolic process sustained 11.1 9.6 9.1 7.8 Cht4 CG3986 chitinase activity - sustained 6.9 5.5 11.8 9.3 CG14957 CG14957 chitin binding + early + 6.6 1.4 2.1 1.5 CG10140 CG10140 chitin binding and chitin metabolic process sustained 6.5 2.3 5.2 2.9 Cry CG16963 constituent of chitin based cuticle - sustained 5.8 2.1 17.2 5 CG6933 CG6933 structural constituent of peritrophic membrane + sustained 4 10.2 1.5 1.3 CG7298 CG7298 structural constituent of peritrophic membrane sustained 3.8 3 2.6 2 CG5883 CG5883 structural constituent of peritrophic membrane - sustained 3.5 2 16.5 1.7 CG7017 CG7017 structural constituent of peritrophic membrane sustained 3.2 3.1 5.7 4.4 CG4835 CG4835 chitin binding and chitin metabolic process / MUCIN + sustained 3.1 3.1 1.2 -1 CG32304 CG32304 chitin binding and chitin metabolic process + sustained 2.5 7.5 1.3 2.9 CG7252 CG7252 structural constituent of peritrophic membrane + sustained 2.3 3.8 1.1 -1.2 Cht9 CG10531 chitinase activity sustained 2.2 3 2.3 3.5 CG7876 CG7876 chitin binding and chitin metabolic process + sustained 2.2 2.4 1.3 -1.1 GUT PHYSIOLOGY (Transporters, digestive enzymes, catabolism) Transporters CG4797 CG4797 glucose transmembrane transporter activity + early 5 1.8 2.3 1.2 rtet CG5760 sugar transmembrane transporter activity early 3.9 1 2.4 -1.1 CG1718 CG1718 transporter acitivity early 3.5 -1.1 3.9 1.3 CG6640 CG6640 transporter activity + early 3.4 1.7 -2.3 -5.2 Oatp58Dc CG3380 organic anion transporter activity early 3.3 -1.4 3.8 -1.3 CG32054 CG32054 carbohydrate transmembrane transporter activity early 3.2 1.2 1.8 -1.1 CG6231 CG6231 carbohydrate transmembrane transporter activity early 2.7 1.4 2.8 1.7 CG8785 CG8785 amine transmembrane transporter activity early 2.7 1.4 2.2 1.8 CG8596 CG8596 transporter activity early 2.7 1.1 2.2 1.1 CG14606 CG14606 Sugar transporter superfamily early 2.4 -1.5 1.8 -2.7 CG3790 CG3790 carnitine transporter activity early 2.3 1.3 2.8 1.6 CG12926 CG12926 transmembrane tranporter activity g sustained 2.2 2 2.8 2.4 CG32053 CG32053 transporter activity early 2.1 1.3 1.9 1.1 CG10300 CG10300 transmembrane tranporter activity early 2.1 -1.2 2.4 1.4 CG7628 CG7628 inorganic phosphate transmembrane transporter activity late 1.5 2 2.5 1.9 CG3394 CG3394 long-chain fatty acid transporter activity late 1.4 2 -1.2 1.5 Ion binding and transport CG3397 CG3397 voltage-gated potassium channel complex + sustained 11.6 2.3 -1.1 -1.3 CG1698 CG1698 cation transmembrane transporter activity + early 5.5 1.8 1.3 -2

CG31530 CG31530 polyspecific organic cation transmembrane transporter activity early 5.2 1.8 5.7 1.4

CG6125 CG6125 high affinity sulfate transmembrane transporter activity early 4.7 1 3.7 -1.1 CG7816 CG7816 metal ion transmembrane transporter activity + early 4.2 1.7 -1 -1.6 CG10641 CG10641 calcium ion binding early + 4.1 1.5 3.6 -1 CG2191 CG2191 cation transmembrane transporter activity sustained 3.1 -2 4.7 -1.1 CG2196 CG2196 cation transmembrane transporter activity early 2.9 -1.6 3.8 -1.4 CG10126 CG10126 calcium ion binding early 2.4 1.5 2.3 1.1 Orct2 CG13610 cation transmembrane transporter activity early 2.2 1.7 1.4 1.1 Best1 CG6264 chloride channel activity early 2.1 1.2 2 1.4 Oatp33Eb CG6417 organic anion transmembrane transporter activity early 2 1.6 1.6 1.2 Oatp58Db CG3382 organic anion transmembrane transporter activity + early 2 -1.4 -1.8 -4.2 CG2187 CG2187 cation transmembrane transporter activity - early 2 -1.7 4.8 -1.1 ppk12 CG10972 sodium channel activity late 1.5 2.9 1.4 2 icln CG4924 volume-sensitive anion channel activity late 1.4 2.2 1.3 2.1 Proteases CG14869 CG14869 metalloendopeptidase activity sustained 5.3 2 3.2 1.6 CG18179 CG18179 chymotrypsin activity - sustained + 4.7 3.4 11.4 9.8 CG7142 CG7142 chymotrypsin activity sustained 4.6 2.5 4 2.3 CG42335 CG31177 membrane alanyl aminopeptidase activity + early 4.3 1.9 2 1.3 Jon66Cii CG7170 elastase activity sustained 3.9 2.8 2.3 1.7 Mmp2 CG1794 metalloendopeptidase activity early 3.8 1.9 3.6 1 CG6337 CG6337 cysteine-type endopeptidase activity sustained 3.1 2 2.3 1.6 CG30090 CG30090 serine-type peptidase activity + early 3.1 1.4 -2.7 -5.9 CG3074 CG3074 cathepsin early 2.8 1.6 2.9 1.3 CG6048 CG6048 trypsin activity early 2.7 1.4 1.6 -1 CG6733 CG6733 metallopeptidase activity early 2.7 -1.5 3.4 1.1 Psa CG1009 aminopeptidase + sustained 2.6 2.1 1.1 1.4 Sras CG4852 prenyl protein specific endopeptidase early 2.5 1.7 1.7 1.3 CG6357 CG6357 cathepsin L early + 2.5 1.3 1.8 1 Jon66Ci CG7118 elastase activity + sustained 2.4 2.6 2 -1.5 CG4650 CG4650 serine-protease early 2.4 1.2 4.2 1.4 CG32155 CG32155 cysteine-type endopeptidase + early 2.3 1.1 -1.3 -1.4 Mmp1 CG4859 metalloendopeptidase activity early 2.3 1.5 4.1 2.9 CG18659 CG18659 serine carboxypeptidase activity early 2 1.4 2.8 1.7 stv CG32130 serine-type endopeptidase activity early 2 1.2 2.6 1.3 pcl CG13374 cathepsin E activity late 1.6 2 -1.1 1.4 CG6372 CG6372 cathepsin E activity + late 1.4 5.9 -1.2 -1.5 Carbohydrate catabolism CG31216 CG31216 hydrolase activity sustained 35 3 24.8 3.6 rev7 CG2948 positive regulation of lyase activity sustained 4.1 2.3 4.5 3.7 Gld CG1152 glucose dehydrogenase activity + early 3.1 1.6 -1.8 -2.3 CG14934 CG14934 alpha glucosidase activity - early 3 1.7 6.4 1.3 CG31148 CG31148 glucosidase activity / hydrolase - early 2.4 1.6 3 3.4 alpha-Man-I CG32684 alpha-mannosidase activity late 1.9 2.4 1.6 1.5 Lipase CG4267 CG4267 phospholipase A1 activity + sustained + 5.8 4.3 1.2 -1.1 CG31272 CG31272 lipase activity early 3.8 -1.9 3.5 -1.9 CG7365 CG7365 phospholipase early 2.7 1.6 2.3 1.7 Miscellaneaous CG3290 CG3290 alkaline phosphatase activity early 3.2 -1.4 2 -2.1 CG31313 CG31313 cysteine protease inhibitor activity sustained 2.5 3.5 2.5 3.7 METABOLISM Carbohydrate metabolism CG16965 CG16965 alpha,alpha-trehalase activity + sustained 6 3.9 1.7 -1 FucTA CG6869 alpha1,3-fucosyltransferase activity + early 4.2 1.9 -1 -1.9 CG33147 CG33147 heparin-glucosamine 3-O-sulfotransferase early 3.9 1.9 3.4 1.3 Gmd CG8890 GDP-mannose 4,6-dehydratase activity early 3.1 1.4 2.9 1.5 pgant3 CG4445 polypeptide GalNAc transferase activity sustained 2.9 2.1 3.4 1.8 CG15818 CG15818 sugar binding + sustained 2.6 2.2 1.1 -1.1 Dhap-at CG4625 Glycerol 3-phosphate acyltransferase activity early 2.2 1.4 2.9 1.5 Glucuronosyltransferase activity Ugt86Di CG6658 glucuronosyltransferase activity + sustained 6 2.5 2.3 1.5 Ugt35b CG6649 UDP-glycosyltransferase early 2.3 -1.9 3.4 -1 Ugt36Ba CG13270 glucuronosyltransferase activity early 2.1 -1.2 2.1 1 Lipid metabolism CG33116 CG33116 CDP alcohol phosphotransferase activity sustained 3.8 2 3.7 1.7 fu12 CG17608 1-acylglycerol-3-phosphate O-acyltransferase activity sustained 3.6 2.3 4.6 1.5 CG7402 CG7402 N acetylgalactosamine-4-sulfatase activity + early 3.2 -1 1.2 -1.1 CG11425 CG11425 phosphatidate phosphatase activity + sustained 2.7 2.2 1.4 1 Spt-I CG4016 serine C-palmitoyltransferase activity early 2.7 1.6 2.4 1.5 CG4825 CG4825 CDP-diacylglycerol-serine O-phosphatidyltransferase activity sustained 2.1 2.2 1.3 1.6 lace CG4162 serine C-palmitoyltransferase activity early 2.1 1.2 2.7 1.3 Acyl-co A metabolism CG15829 CG15829 acyl-CoA binding sustained + 8.6 6.6 8 6.1 CG17108 CG17108 acetyl-CoA carboxylase activity sustained 3.2 3.5 2.8 1.9 CG9009 CG9009 long-chain-fatty-acid-CoA ligase activity sustained 3.2 2.5 3.1 1.8 CG8629 CG8629 acyl-CoA binding sustained 2.5 2.9 2.1 1.9 CG32699 CG32699 acyltransferase activity early 2 1.7 2.5 1.5 yip2 CG4600 acetyl-CoA C-acyltransferase activity early 2 1.4 2.5 1.9 Amino acid and protein metabolism CG12428 CG12428 carnitine O-octanoyltransferase activity early 2.7 1.3 1.7 -1.1 CG14205 CG14205 transferase activity early 2.5 1.3 1.7 1.2 Paps CG8363 adenylylsulfate kinase activity early 2 1.7 1.7 1.5

GlcAT-S CG3881 N-acetyllactosamine beta-1,3-glucuronosyltransferase activity early 2 1.5 2.9 2

CG4433 CG4433 transferase activity, transferring glycosyl groups early 2 1.5 2.3 1.5 CaBP1 CG5809 protein disulfide isomerase activity early 2 -1 2.8 1.4 CG4300 CG4300 S-adenosylmethionine-dependent methyltransferase activity late 1.1 2.2 -1.2 1.6 Nucleic acid metabolism RnrL CG5371 ribonucleoside-diphosphate reductase activity sustained 6.5 3.5 5.2 3.9 CG8353 CG8353 cytidine deaminase activity + early 4.9 1.8 2 1.2 CG8083 CG8083 nucleoside binding early 3.6 -1 3.1 1.1 CG6330 CG6330 uridine phosphorylase activity early 2.1 1.4 3.1 1.6 Other enzymes Uro CG7171 urate oxydase activity - early + 4.5 -1.9 9.4 -1 CG6074 CG6074 carbonate dehydratase activity + sustained 4.4 2.1 2.1 1.1 Ipk2 CG13688 inositol polyphosphate multikinase activity + early 4.3 -1.1 2 -2.1 Mocs1 CG33048 molybdopterin synthase early 4 1.1 4.1 1.4 SH3PX1 CG6757 phosphoinositide binding early 3.3 1.9 3.3 1.8 CG4563 CG4563 4-coumarate-CoA ligase activity + sustained 3.1 2.4 -1.2 -1.3 asparagine-syntheta CG33486 asparagine synthetase activity early 2.3 1.7 1.3 1.5 CG17294 CG17294 4-nitrophenylphosphatase activity early 2 1.3 2.4 2.2 mal CG1692 Mo-molybdopterin cofactor sulfurase activity early 2 1.2 1.4 1.1 Nmnat CG13645 Nicotinamide mononucleotide adenylytransferase activity early 2 1.2 2.2 1.7 CG3940 CG3940 carbonate dehydratase activity early + 2 1 1.8 -1.2 Pect CG5547 ethanolamine-phosphate cytidylyltransferase activity early 2 -1 2.4 1.1 EPITHELIUM RENEWAL Cell cycle/Apoptosis Gadd45 CG11086 homolog of GADD45 early + 5 1.8 2.7 1.5 Ku80 CG18801 ATP-dependent DNA helicase activity early 4.1 1.6 4 2.7 p53 CG10873 p53 homolog early 3.2 1.5 3.8 1.8 Akap200 CG13388 protein kinase A binding early 3 1.9 2.4 1.2 Damm CG18188 caspase activity early 2.4 1.4 1.8 1.1 Eb1 CG40354 receptor binding early 2 1.6 2 1.6 viaf CG18593 protein binding early 2 1.5 2 1.3 mus209 CG9193 PCNA homolog late 1.6 3.2 1.2 2.4 His3 CG31613 histone late 1.6 2.2 1.4 1.8 polo CG12306 spindle assembly late 1.1 2.2 -1.1 1.6 CycE CG3938 cyclin-dependent protein kinase regulator activity late -1.1 3.1 -1.4 1.9 His2B CG17949 histone late -1.1 2.3 -1.3 1.8 Cytoskeleton components betaTub60D CG3401 structural constituent of cytoskeleton sustained 7.8 3.2 4.5 2 act57b CG10067 Actin sustained 4.9 3.1 5.2 3.5 squ HDC02844 unconventional myosin complex sustained 4.8 2.6 2.6 1.8 CG6891 CG6891 actin binding early 4.3 1.9 3.1 1.9 Act42A CG12051 actin filament / structural constituent of cytoskeleton sustained 3.3 2.5 3.4 2.5 zip CG15792 structural constituent of cytoskeleton early 3.1 1.6 3.2 1.8 CG30084 CG30084 actin filament / structural constituent of cytoskeleton early 2.9 1.4 2.5 1.3 act5c CG4027 actin early 2.6 1.9 2.6 2 Jupiter CG31363 colocalizes with microtubule cytoskeleton early 2.4 1.9 2.9 1.7 Rhp CG8497 GTP-Rho binding early 2.4 1.8 3.8 2.5 Vinc CG3299 actin filament / structural constituent of cytoskeleton early 2.4 1.6 2.3 1.4 twf CG3172 actin filament early 2.4 1.5 2.2 1.3 ena CG15112 actin binding early 2.4 1.4 3.4 1.6 cpa CG10540 structural constituent of cytoskeleton early 2.3 1.9 2.4 1.8 cpb CG17158 structural constituent of cytoskeleton early 2.2 1.7 2.2 1.8 cher CG3937 actin binding early 2.2 1.7 2 1.6 sqh CG3595 myosin heavy chain binding early 2.1 1.7 2.2 1.6 CG10724 CG10724 actin binding early 2.1 1.9 2.2 2.1 CG5869 CG5869 actin binding early 2.1 1.5 1.8 1.1 cib CG4944 actin binding early 2.1 1.5 2.3 1.4 Pax CG31794 structural constituent of cytoskeleton early 2 1.5 2.8 1.8 Cell shape and adhesion ImpL2 CG15009 cell adhesion sustained 5.7 3.9 6 5.1 lama CG10645 imaginal disc development sustained 2.6 2.1 2.1 2.2 AnnIX CG5730 annexin early + 2.4 1.8 2.4 1.7 scb CG8095 cell adhesion molecule binding early 2.4 1.6 2.5 1.3 sdt CG32717 guanylate kinase activity / cell polarity early 2.3 1.6 1.9 1.2 Gli CG3903 carboxylesterase activity / ectoderm development early 2.3 1.5 2.9 2 fat2 CG7749 cell adhesion molecule binding early 2.3 1.5 2.7 1.6 mys CG1560 cell adhesion molecule binding early 2.3 1.4 3.1 1.3 wun2 CG8805 phosphatidate phosphatase activity / programmed cell death sustained 2.2 2 1.9 1.5 Cas CG13281 importin-alpha export receptor activity early 2.2 1.6 2.1 1.8 baz CG5055 asymmetric cell division early 2.2 1.5 1.6 -1.2 vanin-like CG32754 pantetheinase activity / cell-cell adhesion / cell motility early 2.2 1.1 1.6 1.5 capt CG5061 actin filament organization early 2.1 1.7 1.9 1.6 cenB1A CG6742 ARF GTPase activator activity early 2.1 1.4 2.3 1.4 Timp CG6281 metalloendopeptidase inhibitor activity early 2.1 1.2 1.9 1.4 cv-2 CG15671 Imaginal disc morphogenesis early 2.1 1.1 2.2 1.5 Lam CG6944 gut morphogenesis early 2 1.5 3 2.1 CG31004 CG31004 cell-matrix adhesion early 2 1.5 2 1.4 Anxb11 CG9968 annexin early 2 1.2 2 1.3 Wounding NijA CG6449 tissue regeneration / integral to membrane sustained + 4.9 2 3.9 1.5 CG5550 CG5550 fibrinogen-like sustained + 4.7 2.1 3.5 1.1 CG7668 CG7668 Unknwon, fibrinogen domain early 2.3 1.1 1.6 1.1 Replication / Repair / recombination RpA-70 CG9633 DNA-dependent DNA replication sustained 3.9 2 4.4 3 Irbp CG5247 ATP-dependent DNA helicase activity early 3.7 1.5 4.7 2.6 CG15220 CG15220 DNA replication factor A complex sustained 3.5 2.6 3.9 3.7 Ercc1 CG10215 damaged DNA binding early 3 1.4 3 1.7 CG9273 CG9273 DNA replication factor A complex sustained 2.8 2.8 2.4 2.6 mre11 CG16928 endodeoxyribonuclease activity / double-strand break repair early 2.7 1.4 2.5 2.1 CG17227 CG17227 DNA ligase (ATP) activity / DNA repair early 2.6 1.2 2.7 1.7 Lig4 CG12176 DNA ligase (ATP) activity / DNA repair early 2.4 1.2 2.6 1.6 RfC40 CG14999 DNA-directed DNA polymerase activity early 2.3 1.6 2.5 2.2 XRCC1 CG4208 damaged DNA binding / Nucleotide excision repair early 2.2 1.3 2.4 1.4 CG31953 CG31953 DNA repair early 2.1 1.5 2.4 1.8 Tom34 CG2708 DNA ligase (ATP) activity / DNA repair early 2.1 1.2 2.9 1.9 DDB1 CG7769 Nucleotide excision repair early 2 1.5 2.5 2.2 CG10898 CG10898 DNA repair late 1.3 2.1 1.2 2.9 Rrp1 CG3178 3'-5' exonuclease activity / DNA repair late 1.2 2 1.2 2.3 Mcm3 CG4206 3'-5' DNA helicase activity / pre replicative complex late 1.1 2.5 -1.1 1.7 dpa CG1616 3'-5' DNA helicase activity / DNA replication late 1 2.1 -1.1 2.1 Mcm7 CG4978 3'-5' DNA helicase activity / DNA replication initiation late -1 3 -1.3 2 Mcm2 CG7538 3'-5' DNA helicase activity / DNA replication initiation late -1.1 2.1 -1.2 1.5 DNApol-alpha180 CG6349 3'-5'-exodeoxyribonuclease activity / replication late -1.1 2.1 -1.3 1.6 Mcm5 CG4082 3'-5' DNA helicase activity / DNA replication initiation late -1.2 2.6 -1.4 1.8 Tetraspanins Tsp42Ef CG12845 integral to membrane early 5.4 1.7 6.5 1.7 Tsp42Ei CG12843 integral to membrane sustained 3.3 2.6 5.5 3.6 Tsp42Eb CG30160 unknown early 2.6 1.6 2 1.1 Tsp86D CG4591 cell-cell adhesion early 2.4 1.3 2.2 1.2 Tsp42Ea CG18817 integral to membrane early 2 1.5 2.2 1.3 GENE REGULATION Transcription factor slbo CG4354 transcription factor activity / cell fate specification + early 4.1 1.9 1.4 -1.1 La CG10922 RNA polymerase III transcription termination factor activity early 3.1 1.8 3.1 2.6 kay CG15509 transcription factor activity early 2.8 1.6 2.1 1.1 CG6769 CG6769 Zn finger protein early 2.6 1.2 2.4 1.8 CG6272 CG6272 transcription factor activity early 2.3 1.3 2.4 1.6 NK7.1 CG8524 transcription factor activity early 2.1 1.5 1.7 1.2 Trf2 CG18009 specific RNA polymerase II transcription factor activity early 2.1 1.4 1.9 1.3 chromatin regulator DNA metabolism Ote CG5581 nuclear envelope reassembly + sustained 7.1 3.7 3 2.1 Ssrp CG4817 DNA secondary structure binding sustained 3 2.1 3.7 3.3 agt CG1303 DNA-methyltransferase activity early 2.8 1.5 2.9 2.6 escl CG5202 histone methyl transferase complex early 2.3 1.3 3.2 1.8 Gp93 CG5520 DNA topoisomerase (ATP-hydrolyzing) activity early 2.2 1.1 2.3 1.5 CG3168 CG3168 DNA topoisomerase (ATP-hydrolyzing) activity early 2.2 -1.6 3.4 -1.1 dnk CG5452 deoxynucleoside kinase activity sustained 2 2.4 1.6 1.8 CG12177 CG12177 nucleic acid metabolic process late 1.7 2.4 1.4 1.9 asf1 CG9383 chromatin architecture late 1.5 2.2 1.6 1.8 Ant2 CG1683 nucleic acid metabolic process late 1.4 2.2 1.6 1.9 dUTPase CG4584 dUTP diphosphatase activity late 1.2 2.8 1.1 2.6 CG6854 CG6854 CTP synthase activity late 1.2 2 -1.3 1.2 CG31453 CG31453 ATP binding late 1.2 2.2 -1.2 1.6 CG17266 CG17266 peptidyl-prolyl cis-trans isomerase activity late -1 2 -1.1 1.8 DNApol-!50 CG7108 alpha DNA polymerase activity late -1.1 2.2 -1.1 1.6 RnrS CG8975 ribonucleoside-diphosphate reductase activity late -1.1 2.2 -1.4 1.5 RNA regulation, splicing, translation, Protein regulation CG6967 CG6967 mRNA metabolism / NMD + sustained 4.3 3.6 -1.2 -1.1 alan-shepard CG32423 mRNA processing early 3.2 1.7 2.2 1.3 mkg-p CG7163 tRNA splicing early 3 1.5 2.8 1.6 CG5800 CG5800 RNA helicase early 2.8 1.5 2.6 2 eIF6 CG17611 translation initiation factor activity early 2.7 1.3 2.5 2 Nxt1 CG12752 mRNA export from nucleus sustained 2.5 2.1 2.7 2.5 CG33057 CG33057 2'-phosphotransferase activity early 2.5 1.5 2.4 1.6 CG5205 CG5205 ATP-dependent helicase activity early 2.5 1.4 2.7 1.8 CG12288 CG12288 mRNA binding sustained 2.3 2.3 1.2 2.1 CG15100 CG15100 methionine-tRNA ligase activity early 2.3 1.4 1.9 1.5 CG6876 CG6876 RNA splicing factor activity, transesterification mechanism early 2.3 1.3 2.3 1.9 JhI-1 CG3298 endoribonuclease activity, producing 5'-phosphomonoesters early 2.2 1.3 1.6 1.3 Cctgamma CG8977 protein folding / chaperonin-containing T-complex early 2.2 1.6 2.2 1.7 Pabp2 CG2163 RNA binding / mRNA polyadenylation early + 2.2 1.4 1.6 1.2 heph CG31000 nucleotide binding sustained 2.1 2 1.7 1.2 CG11454 CG11454 mRNA binding early 2.1 1.3 2 1.2 HBS1 CG1898 translation elongation factor activity early 2 1.2 2.5 1.9 DebB CG16792 RNA splicing factor activity, transesterification mechanism late 1.2 2 1.2 1.8 NEURONAL TRANSMISSION / HORMONES Dh CG8348 diuretic hormone activity early + 6.1 1.6 6.9 1.8 wfs1 CG4917 glutamate-cysteine ligase activity early 3.9 1.8 3.8 2.3 nemy CG8772 glutaminase activity / locomotory behavior early 3.8 1.4 6.1 2.2 burs CG13419 neuropeptide hormone activity sustained 3.5 2 1.9 1.6 Syx4 CG2715 SNAP receptor activity / neurotransmitter secretion early 3.1 1.5 2.1 1.1 bnb CG7088 gliogenesis early 2.8 1.8 5.2 2.3 gammaSnap CG3988 neurotransmitter secretion early 2.7 1.6 2.8 1.6 sda CG5518 mechanosensory behavior early 2.5 1.5 1.7 -1.1 melt CG8624 insulin receptor signaling pathway early 2.5 1.4 1.3 1.2 CG5921 CG5921 sensory perception early 2.4 1.8 1.4 1.5 Shaw CG2822 voltage-gated potassium channel activity early 2.4 1.4 2.1 1.4 Gr94a CG31280 taste receptor activity / sensory perception of taste early 2.1 1.4 2.6 2.1 cib CG4944 actin binding / brain development early 2 1.8 2.1 1.5 Obp56d CG11218 odorant binding / olfactory behaviour late 1.7 5.3 2.5 3.9 AlstR CG2872 allostatin receptor + late 1.1 2.2 -1.1 1 MISCELLANEOUS UbcD2 CG6720 ubiquitin-protein ligase activity early 2.8 1.5 2.2 1.2 CG4780 CG4780 intra-Golgi vesicle-mediated transport early 2.1 1.1 2 1.2 Pen CG4799 protein carrier activity / protein import into nucleus late -1 3.2 -1.4 2.2 UNKNOWN GENES CG8620 CG8620 unknown sustained 20.5 4 13.5 6.5 CG4367 CG4367 unknown + early 19.5 1.1 1.1 1.1 CG8317 CG8317 unknown + sustained 18.7 10.3 -1.1 1 CG9080 CG9080 unknown + sustained + 18.5 48.3 -2.1 -2 CG15282 CG15282 unknown + sustained + 17.2 29.8 -1 -1.2 CG2909 CG2909 unknown - sustained 16 2.9 18.1 5.8 CG13325 CG13325 unknown - early + 14.7 1.5 40.8 4.1 CG14219 CG14219 unknown sustained + 12.6 2.7 10.8 4.2 CG4269 CG4269 unknown + sustained + 11.4 4.3 -1.4 -2.2 CG3448 CG3448 unknown sustained 10.7 3.4 12.3 5.2 CG14695 CG14695 unknown - sustained 10.6 3.7 12.7 8.4 CG33468 CG33468 unknown - sustained 8.5 2.7 17.2 3.6 CG31789 CG31789 unknown + sustained 8.2 56.5 -1.6 -1.5 CG10182 CG10182 unknown sustained 7.7 3.6 7.7 3.7 CG3703 CG3703 unknown + sustained 7.4 2.3 1.9 1.2 CG6429 CG6429 unknown + sustained + 7.4 3.1 1.4 1.1 CG13941 CG13941 unknown sustained + 6.9 3.1 6.3 3.1 CG5630 CG5630 unknown - sustained 6.2 2.7 11.1 3.9 CG7296 CG7296 unknown + sustained + 5.6 2.9 -1 -1.7 CG7738 CG7738 unknown + sustained + 5.5 19.8 -1.3 -2.1 CG31041 CG31041 unknown early 5.4 1.2 3.2 1.8 CG33510 CG14398 unknown - early 5.2 1.7 9.9 3.9 CG13324 CG13324 unknown sustained + 5.2 2.1 4.9 2 CG18213 CG18213 unknown - early 5.1 1.8 8 4.1 CG15893 CG15893 unknown early 5 1.6 4.9 1.5 CG14949 CG14949 unknown sustained 4.8 2.1 4 1.7 CG32195 CG32195 unknown - early 4.8 1.6 10.9 6.7 CG14906 CG14906 unknown sustained 4.7 2.1 5.6 3 CG1890 CG1890 unknown sustained 4.6 2.1 4.3 2.3 CG16969 CG16969 unknown early 4.5 1.5 5.8 2.9 CG11470 CG11470 unknown + early 4.2 1.7 1.2 1 CG13077 CG13077 unknown + sustained + 4.1 2.5 -1.1 -1.1 CG1143 CG1143 unknown sustained 4.1 2 3.3 1.5 CG12868 CG12868 unknown early 4.1 1.5 3.8 1.8 CG5765 CG5765 unknown + sustained + 4 2.4 1.8 1.5 CG14339 CG14339 unknown sustained 4 2.3 4.9 4 CG13075 CG13075 unknown early 4 1.7 2.8 1.1 CG10560 CG10560 unknown early 4 -1.1 4.4 -1.1 CG13488 CG13488 unknown + sustained 3.9 3.9 1 1.3 CG10337 CG10337 unknown sustained 3.7 2.3 2.2 1.7 CG14879 CG14879 unknown early 3.7 1.5 3.6 1.8 CG31087 CG31087 unknown sustained 3.6 4.4 3.1 4.3 CG3792 CG3792 unknown early 3.6 1.7 3.8 1.6 CG13420 CG13420 unknown + early 3.6 1.8 1.7 1.2 CG31633 CG31633 unknown early 3.5 1.4 3.6 1.8 CG14872 CG14872 unknown - early 3.4 1.2 8.1 3 CG14204 CG14204 unknown early 3.3 1.2 1.7 1.3 CG9988 CG9988 unknown sustained 3.2 2.2 2.8 3.5 CG8678 CG8678 unknown early 3.2 1.9 2.7 1.4 CG5664 CG5664 unknown early 3.2 1.6 3.3 2.2 CG32224 CG32224 unknown early 3.2 1.1 5.5 1.1 CG12112 CG12112 unknown early 3.2 1.8 3.3 1.6 CG3165 CG3165 unknown early 3.1 1.6 2.6 1.3 CR18748 CG18748 unknown early 3.1 1.2 1.7 1.1 CG6171 CG6171 unknown early 3.1 1.4 4 2.5 CG31344 CG31344 unknown sustained 3 2.7 2.4 2.1 CG9987 CG9987 unknown early 3 1.7 3.1 2.3 CG31694 CG31694 unknown early 3 1.6 4 2.3 CG13654 CG13654 unknown early + 3 1.4 2.9 1 Ect4 CG7915 unknown early 3 1.2 2.3 1.1 CG15210 CG15210 unknown sustained 3 3.2 2.7 2.2 CG16775 CG16775 unknown + sustained 3 2.4 1.6 1 CG31864 CG5202 unknown early 3 1.3 3.1 2.1 CG12970 CG12970 unknown sustained 2.9 3 2.1 2.3 CG15083 CG15083 unknown sustained 2.9 2.6 2.3 1.7 CG18596 CG18596 unknown early 2.9 1.5 3 1.9 ome CG32143 unknown early 2.9 1.3 3.9 1.7 CG32107 CG32107 unknown + early 2.9 -1 1.2 -2.5 CG32185 CG32185 unknown + sustained 2.9 4 -1.1 -1.2 CG18212 CG18212 unknown early 2.8 1.6 1.7 1.1 CG10660 CG10660 unknown early 2.8 1.6 2.2 1.3 CG31324 CG31324 unknown early 2.8 1.6 2.5 1.2 CG9646 CG9646 unknown early 2.8 1.4 2.4 1.5 CG10062 CG10062 unknown early 2.8 1.4 2.4 1.1 CG32485 CG32485 unknown early 2.8 1.3 2.6 1.1 CG11750 CG11750 unknown early 2.8 1.2 2.7 -1.1 CG11686 CG11686 unknown early 2.7 1.9 2.4 1.6 CG15211 CG15211 unknown early 2.7 1.5 2.8 1.5 CG4080 CG4080 unknown early 2.7 1.4 1.6 1.3 CG32713 CG32713 unknown early 2.7 1.4 2.3 1.9 c11.1 CG12132 unknown early 2.7 1.3 2.5 1.5 CHKov2 CG10675 unknown early 2.7 1.2 2.5 1.4 CG4351 CG4351 unknown early 2.7 1.1 2.6 1.1 CG1572 CG1572 unknown early 2.6 1.8 2.6 1.4 CG31436 CG31436 unknown + early 2.6 1.7 -3.6 -5.6 bocksbeutel CG9424 unknown early 2.6 1.3 3.4 1.9 CG7272 CG7272 unknown early 2.6 -1.2 2.3 1 CG34043 Dm.2L.8013.0 unknown early 2.6 -1.5 4.2 -1.3 CG31686 CG31686 unknown + sustained 2.6 3.4 1.3 1 CG13482 CG13482 unknown early 2.6 1.8 2.2 1.1 CG33469 CG33469 unknown early 2.6 1.8 2.5 2 CG7810 CG7810 unknown early 2.6 1.6 2.6 1.6 CG13323 CG13323 unknown early + 2.6 1.5 1.5 1.4 CG14193 CG14193 unknown early 2.6 1.4 1.9 1.1 DyakCG13335 CG13335 unknown + sustained 2.5 2.9 1.2 -1.8 CG11378 CG11378 unknown + sustained 2.5 2 1.3 -1 Dlit CG17667 unknown early 2.5 1.8 1.9 1.3 CG5910 CG5910 unknown early 2.5 1.4 2 1.2 CG15281 CG15281 unknown early + 2.5 1.2 1.9 2.9 MESK4 CG31447 unknown early 2.5 -1.1 1.7 -1.2 CG5446 CG5446 unknown early 2.5 1.7 1.5 1.1 CG15044 CG15044 unknown + early 2.5 1.4 1.3 -1.7 CG10559 CG10559 unknown + sustained 2.4 6.1 -1.8 -1.4 CG13252 CG13252 unknown early 2.4 1.8 1.2 1 CG8209 CG8209 unknown early 2.4 1.7 3 2.3 CG33107 CG33107 unknown early 2.4 1.6 2.4 1.8 CG10916 CG10916 unknown early 2.4 1.5 2.1 1.4 CG14907 CG14907 unknown early + 2.4 1.5 2.7 1.9 CG3884 CG3884 unknown early 2.4 1.5 2.1 1.4 CG30193 CG30193 unknown + early 2.4 1.4 1 1.1 CG13905 CG13905 unknown sustained + 2.4 -2.3 3.8 -2.7 CG18067 CG18067 unknown - early + 2.3 1.8 4.1 4 CG10345 CG10345 unknown early 2.3 1.7 1.7 1.5 CG10086 CG10086 unknown early 2.3 1.7 1.2 -1 CG18635 CG18635 unknown early 2.3 1.5 2.4 1.4 mthl14 CG32476 unknown early 2.3 1.4 2 1.2 CG5953 CG5953 unknown early 2.3 1.1 2.1 1.1 CG7874 CG7874 unknown - sustained 2.3 -2 5.2 -1 CG17325 CG17325 unknown + sustained 2.3 2.9 -1.2 1.4 CG10570 CG10570 unknown + sustained 2.3 2.7 -1.7 1.2 CG15423 CG15423 unknown sustained 2.3 2.1 1.6 1.1 CG31955 CG31955 unknown sustained 2.3 2.1 2.5 1.6 CG13506 CG13506 unknown early 2.2 1.8 2.7 1.7 CG13321 CG13321 unknown early 2.2 1.7 2.2 1.6 CG5084 CG5084 unknown early 2.2 1.5 2.6 1.6 CG13512 CG13512 unknown early 2.2 1.5 2.6 1.5 bip1 CG7574 unknown early 2.2 1.5 2.3 1.6 CG9996 CG9996 unknown + early 2.2 1.4 -1.1 -1 CG6014 CG6014 unknown early 2.2 1.4 1.7 1.3 CG6353 CG6353 unknown early 2.2 1.3 4.2 2.1 CG6066 CG6066 unknown early 2.2 1.3 2.7 1.9 CG18744 CG18744 unknown early 2.2 1.2 2.3 1.2 CG33258 CR33258 unknown early 2.2 -1.1 1.9 -1.7 CG12918 CG12918 unknown early 2.2 -1.5 2.7 1.2 CG13311 CG13311 unknown - early + 2.2 -1.6 5.5 -1 CG17570 CT34146 unknown sustained 2.2 2.2 1.2 1.2 CG15127 CG15127 unknown sustained 2.2 2.1 1.9 1.5 CG13511 CG13511 unknown early 2.2 1.6 3.4 2.2 CG32023 CG32023 unknown early 2.2 -1.3 2.8 -1.4 CG33109 CG33109 unknown + sustained + 2.1 4.8 -70.8 -19.2 CG15784 CG15784 unknown early + 2.1 1.8 1.8 1.8 CG32368 CG32368 unknown early 2.1 1.8 1.4 1.2 CG5734 CG5734 unknown early 2.1 1.6 2.1 1.1 CG11242 CG11242 unknown + early 2.1 1.5 -1.5 -1.7 CG1105 CG1105 unknown early 2.1 1.5 2.6 1.4 CG4781 CG4781 unknown early 2.1 1.5 1.7 1.1 CG10550 CG10550 unknown early 2.1 1.4 1.8 2 CG2310 CG2310 unknown early 2.1 1.4 1.6 -1 CG32579 CG32579 unknown early 2.1 1.3 2.1 1.6 CG10943 CG10943 unknown + early 2.1 1.3 -1.1 -1.8 CG1407 CG1407 unknown early 2.1 1.3 2.1 1.1 CG11847 CG11847 unknown early 2.1 1.2 2 1.5 CG11141 CG11141 unknown + early 2.1 1.2 -1.1 -1.7 CG32177 CG32177 unknown early 2.1 1.2 2.8 1.6 CG12818 CG12818 unknown early 2.1 1.1 1.1 1.1 CG1623 CG1623 unknown early 2.1 1.1 2 1.2 CG10973 CG10973 unknown early 2.1 1.1 2.5 1.4 CG10102 CG10102 unknown sustained + 2.1 2.2 2 2.7 CG14302 CG14302 unknown sustained 2.1 2 1.7 3.6 CG13510 CG13510 unknown early 2.1 1.6 3.7 2.2

Table S7

D Fold change compared to I R

Gene Symbol CG number Molecular function time G wild type uninfected guts Rel response Or 4h Or 16h Rel 4h Rel 16h

ANTIMICROBIAL DEFENSE Recognition lectin-24A CG3410 lectin sustained -5.5 -5.3 -7 -5.9 SIGNALING PATHWAYS wit CG10776 protein kinase activity / TGF beta receptor activity early -3.5 -1.7 -4.3 -1.9 Toll-9 CG5528 transmembrane receptor activity / signal transduction sustained -3.3 -2.6 -3.6 -1.9 CG3277 CG3277 tyrosine kinase activity early -2.9 -1.5 -2.6 -1.6 CG6560 CG6560 GTPase activity early -2.9 -1.6 -1.8 1.1 Src64B CG7524 protein-tyrosine kinase early -2.7 -1.2 -2.1 -1.2 Galpha73B CG12232 GTPase activity early -2.7 -1.8 -2.3 -1.4 cdi CG6027 serine threonine kinase activity early -2.3 -1.3 -2.7 -2 tkv CG14026 protein kinase activity early -2.3 -1.6 -1.8 -1.3 CG32687 CG32687 serine threonine kinase activity early -2.3 -1.4 -2.4 -1.3 Lobe CG10109 negative regulation of JNK cascade early -2.1 -1.5 -3.2 -2.2 ACXD CG5712 G-protein coupled receptor protein signaling pathway early -2.1 -1.2 -2.8 -1.7 EDTP CG6542 protein tyrosine phosphatase activity early -2.1 -1.2 -2.6 -1.6 S6k CG10539 receptor signaling protein serine/insulin signaling pathway early -2 -1.5 -2.5 -1.8 Marf CG3869 GTPase activity early -2 -1.4 -2.2 -1.6 fz3 CG16785 Wnt receptor activity early -2 -1.2 -1.8 -1.2 STRESS RESPONSE / DETOXIFICATION Cytochrome P450 Cyp4d14 CG3540 cytochrome P450 sustained + -6.9 -4.7 -4.1 -4.1 Cyp4ac1 CG14032 cytochrome P450 - sustained + -6.9 -5.7 -2.2 -1.7 Cyp6t1 CG1644 cytochrome P450 sustained -6.4 -2.6 -9 -3.8 Cyp6g1 CG8453 cytochrome P450 sustained + -5.3 -2.8 -2.9 -2.9 Cyp28d1 CG10833 cytochrome P450 - sustained + -4.9 -4.8 1.3 1.2 Cyp12d1-d CG18240 cytochrome P450 sustained -3.2 -4.3 -2.2 -2.5 Cyp12d1-d/-p CG30489 cytochrome P450 sustained -3 -3.7 -2.2 -2.2 Cyp4d1 CG3656 cytochrome P450 sustained -2.9 -2.6 -4 -3.1 Cyp28a5 CG8864 cytochrome P450 sustained -2.9 -3.1 -3.6 -2.6 Cyp6d5 CG3050 cytochrome P450 early -2.7 -1.8 -1.7 -1.4 Cyp12a4 CG6042 cytochrome P450 early -2.7 -1.9 -2.5 -1.6 Cyp4d8 CG4321 cytochrome P450 early -2.6 -1.3 -3.6 -2.3 Cyp12a5 CG11821 cytochrome P450 sustained -2.6 -2 -2.3 -1.5 Cyp4ae1 CG10755 cytochrome P450 early -2.6 -1.7 -2.1 -1.4 Cyp6a9 CG10246 cytochrome P450 early -2.3 -1.7 -2.2 -2.5 Cyp6a19 CG10243 cytochrome P450 early -2.1 -1.3 -2.3 -2 Cyp9f2 CG11466 cytochrome P450 early -2.1 -1.4 -1.5 -1.1 Cyp9b1 CG4485 cytochrome P450 - sustained -2.1 -2.6 3.4 2.3 Cyp6a2 CG9438 cytochrome P450 - sustained -2 -3.9 1.8 -1.1 Cyp4e2 CG2060 cytochrome P450 late + -1.2 -2.2 1.2 -2 Glutathione transferase activity GstE9 CG17534 glutathione transferase activity early -2 -1.8 -1.4 -1 CG6673 CG6673 glutathione transferase activity early -2.2 -1.1 -1.9 -1.2 GstE8/GstE9 CG17533 glutathione transferase activity early -2.3 -1.5 -2.4 -1.9 CG1681 CG1681 glutathione transferase activity sustained -5.1 -2.3 -4.1 -3.4 Oxydative stress response Eip71CD CG7266 methionine-S-sulfoxide reductase activity - sustained -4.4 -6.9 -3.1 -2.7 CG3301 CG3301 oxidoreductase activity early + -4.2 -1.9 -3.9 -2.6 CG31075 CG31075 aldehyde dehydrogenase activity - early -4 -1.9 1.3 1.2 pxt CG7660 peroxydase activity early -3 -1.2 -3 -1.2 CG3609 CG3609 oxidoreductase activity early + -2.6 -1.8 -1.8 -1.5 Fmo-2 CG3174 monooxygenase activity early -2.6 -1.8 -3.3 -1.9 CG3699 CG3699 oxidoreductase activity early + -2.6 -1.9 -1.7 -1.4 CG3270 CG3270 oxidoreductase activity sustained -2 -2.8 -1.4 -2.1 CG7322 CG7322 oxidoreductase activity late + -1.8 -2 -1.2 -1.6 CG6012 CG6012 oxidoreductase activity - late -1.8 -2 -1.3 -1 CG9914 CG9914 oxidoreductase activity - late + -1.7 -3.2 1.2 -2.1 CG9629 CG9629 aldehyde dehydrogenase late -1.4 -2.2 1.3 -1.2 Miscellaneous Mdr50 CG8523 export of toxic compounds sustained -2 -2.2 -1.7 -1.5 Peritrophic matrix constituant / Chitin metabolism / Constituant of chorion Vm34Ca CG9271 constituant of vitelline membrane early -4.2 1.3 -4.4 1.1 Cp38 CG11213 structural constituant of chorion early -3.8 -1.3 -5.6 -1.3 Vm26Aa CG9048 constituant of vitelline membrane early -3.7 1.6 -3.8 -1.1 Vm26Ab CG9046 constituant of vitelline membrane + sustained -3.5 2 -4.7 1 CG17147 CG17147 structural constituant of peritrophic matrix + sustained -3.2 -3.5 -182.3 -78.8 Vm32E CG16874 constituant of vitelline membrane early -2.4 -1 -2.5 -1.1 Lcp1 CG11650 structural constituent of chitin-based cuticle - late -1.5 -2.3 3.5 1.8 CG10725 CG10725 structural constituent of peritrophic membrane - late 1.5 -3.1 81.2 49.4 CG32656 CG32656 chitin metabolic process late 1.5 -2.5 2.2 -1.3 GUT PHYSIOLOGY (Transporters, Digestive Enzymes, Catabolism) transporters CG31636 CG31636 transmembrane transporter activity - sustained -20.2 -6.8 -13.9 -2.4 CG1208 CG1208 hexose transporter activity sustained -6.1 -3.5 -4.3 -2.7 CG9664 CG9664 transporter activity early -4.4 -1.6 -5.5 -1.7 CG5254 CG5254 transmembrane transporter activity sustained -3.6 -2.3 -2.4 -2 CG17664 CG17664 water channel activity sustained -3.4 -2.1 -2.9 -2.4 CG4019 CG4019 water channel activity early + -3.2 -1.5 -3 -1.8 CG13907 CG13907 monocarboxylate transmembrane transporter activity early -2.7 -1.2 -1.9 -1.1 CG12194 CG12194 transporter activity sustained -2.5 -2.3 -2.1 -2.2 CG18347 CG18347 transmembrane transporter activity early -2.5 -1.9 -1.8 -1.8 Sug CG7334 transporter activity sustained -2.3 -2.1 -3.1 -2.3 CG8026 CG8026 folic acid transporter activity early -2.2 -1.2 -2.4 -1.2 CG18317 CG18317 transmembrane transporter activity early -2.2 -1.3 -2.2 -1.2 CG8925 CG8925 carnitine transporter activity early -2.1 -1.4 -2.6 -2.4 CG4630 CG4630 carbohydrate transmembrane transporter activity + early -2.1 -1.5 -6.9 -6.4 Oatp33Ea CG5427 organic anion transmembrane transporter activity early -2.1 -1.5 -1.9 -1.2 CG8062 CG8062 monocarboxylic acid transmembrane transporter activity early -2 -1.3 -1.3 -1.1 CG1139 CG1139 amine transmembrane transporter activity sustained -2 -2.7 -1.6 -3.5 Fatp CG7400 long-chain fatty acid transporter activity early -2 -1.5 -1.7 -1.5 CG6484 CG6484 glucose transmembrane transporter activity early -2 -1.3 -1.2 -1 frc CG3874 UDP-N-acetylglucosamine transmembrane transporter activity early -2 -1.2 -1.9 -1.3 CG1628 CG1628 L-ornithine transmembrane transporter activity early -2 -1.9 -2 -1.8 CG11407 CG11407 long-chain fatty acid transporter activity - sustained + -2 -3.2 1.4 1.1 CG10505 CG10505 transporter activity late -1.3 -2 -1.2 -1.8 l(2)01810 CG5304 sodium symporter activity late -1.3 -2.1 -1.5 -1.9 CG4830 CG4830 long-chain fatty acid transporter activity late -1 -5.7 1.6 -7.2 CG15406 CG15406 fructose transmembrane transporter activity late 1.1 -2.1 1.8 -1.1 Ion binding and transport smp-30 CG7390 calcium binding activity - sustained + -6.8 -2.8 -2.1 -3.3 CG9903 CG9903 sodium symporter activity - sustained -6.2 -2.4 -3 -1.3 CG31116 CG31116 chloride transport sustained -4.2 -2.4 -3.4 -2.3 CG9981 CG9981 cation transport - sustained -4.1 -4.5 -2.6 -2.2 CG9864 CG9864 high affinity inorganic phosphate:sodium symporter activity sustained -3.7 -2.6 -2.3 -2.4 ZIP1 CG9428 cation transporter activity sustained -3.6 -2 -2.4 1.1 Mct1 CG3456 monocarboxylic acid transmembrane transporter activity early -3.2 -1.2 -3.2 -1.3 CG7881 CG7881 high affinity inorganic phosphate:sodium symporter activity sustained -3 -3.5 -3.1 -2.8 CG32669 CG32669 cation transmembrane transporter activity early -3 -1.6 -3.1 -1.5 MtnC CG5097 metal ion binding early + -2.9 -1.5 -2.2 -2 CG8468 CG8468 cation tranporter activity - sustained -2.9 -2.7 -1.8 -1.2 CG1600 CG1600 Ion binding sustained + -2.8 -2.1 -1.5 -1.6 CG9825 CG9825 high affinity inorganic phosphate:sodium symporter activity + sustained -2.7 -2.5 -6.1 -13.8 CG7966 CG7966 selenium binding activity sustained -2.4 -2 -1.7 -1.6 Yippee CG1989 metal ion binding early -2.3 -1.4 -2 -1.2 MtnD CG33192 metal ion binding early -2.3 1.7 -2.2 1.7 MtnA CG9470 metal ion binding early -2.2 -1.1 -2.8 -1.3 Ca-P60A CG3725 calcium transporting GTPase activity early -2.2 -1.3 -1.9 -1.2 CG12602 CG12602 hydrogen-exporting ATPase activity early -2.1 -1.5 -1.6 -1.1 Ndae1 CG4675 anion exchanger activity early -2.1 -1.6 -2.3 -2.1 CG8177 CG8177 inorganic anion exchanger activity early -2.1 -1.7 -4 -2.3 CG9430 CG9430 metal ion transmembrane transporter activity - early -2.1 -1.6 1.3 2.7 Scp1 CG15848 calcium ion binding early -2 -1.1 -2.3 -1.1 CG30492 CG30492 zinc ion binding early -2 -1.1 -1.8 -1.1 CG9826 CG9826 high affinity inorganic phosphate:sodium symporter activity + early -2 -1.7 -3.3 -4.6 Oatp58Da CG30277 organic anion transmembrane transporter activity - sustained -2 -2.1 3 2 CG8791 CG8791 high affinity inorganic phosphate:sodium symporter activity late + -1.9 -2 -1.9 -2.3 Proteases Ser8 CG4812 serine-type endopeptidase activity - sustained + -19.2 -4.6 -6.3 -2 CG7025 CG7025 carboxypeptidase activity - sustained -9.3 -12.1 -4.1 -2.8 iotaTry CG7754 trypsin activity sustained -4.6 -4.3 -4.9 -2.3 Nep1 CG5905 metalloendopeptidase activity sustained -4.3 -2.6 -4.9 -2.2 alpha-Est7 CG1112 carboxyl esterase activity sustained -3.7 -2.2 -2.2 -1.5 CG3739 CG3739 serine-type endopeptidase activity + sustained -3.3 -2.4 -6.5 -2.7 CG3622 CG3622 metalloendopeptidase activity early -3.3 -1.8 -2.7 -2.1 alpha-Est4 CG1082 carboxyl esterase activity sustained -3.2 -2.4 -4.1 -3.3 alpha-Est1 CG1031 carboxyl esterase activity sustained -3.2 -2.4 -3.2 -2.3 CG8773 CG8773 glutamyl aminopeptidase activity sustained -3.1 -2.8 -2.9 -2.3 CG8562 CG8562 metallocarboxypeptidase activity early + -2.7 -1.7 -3.3 -1.4 CG9634 CG9634 metalloendopeptidase activity early -2.6 -1.4 -2.5 -1.4 Dip-C CG5663 X-pro dipepetidase activity early -2.6 -1.4 -3.1 -2.1 CG15253 CG15253 metalloendopeptidase activity + sustained -2.6 -2 -6.2 -2 CG3734 CG3734 serine-type endopeptidase activity early -2.5 -1.6 -2.6 -1.5 CG4752 CG4752 5 oxo prolinase activity early -2.5 -1.9 -1.6 -1.4 CG11912 CG11912 serine-type endopeptidase activity sustained -2.1 -2 -2.1 -1.4 thetaTry CG12385 trypsin activity sustained + -2.1 -2.4 -1.7 -1.8 CG16749 CG16749 serine-type endopeptidase activity sustained + -2.1 -2.5 -1.6 -1.5 lambdaTry CG12350 trypsin activity early -2 -1.9 -2.1 -1.3 CG9953 CG9953 serine-type Pro-X carboxypeptidase activity early -2 -1.1 -1.6 1 CG31823 CG31823 serine carboxypeptidase activity late -1.9 -2 -1.6 -1.8 CG17477 CG17477 chymotrypsin activity late -1.9 -2 -2.6 -3.2 CG8560 CG8560 carboxypeptidase A activity late -1.9 -2 -2.8 -2 nmo CG3841 carboxylesterase activity late -1.6 -2 -1.1 -1.2 Ser6 CG2071 serine-type endopeptidase activity - late -1.5 -2.2 1.7 1.9 Jon25Bii CG8869 elastase activity + late + -1.5 -2.1 -1.8 -7 Jon65Aii CG6580 elastase activity + late -1.4 -2.3 -3.5 -3.1 Jon99Ci CG31039 elastase activity late -1.2 -2.1 -1.1 -1.5 CG8299 CG8299 trypsin activity late -1 -2.1 1.2 -1.3 CG1304 CG1304 serine-type endopeptidase activity - late 1 -4.4 29.8 6.5 Carbohydrate catabolism CG9465 CG9465 alpha mannosidase activity sustained -10.1 -8.2 -5.8 -8 CG9463 CG9463 alpha mannosidase activity - sustained + -7.1 -13.8 -2.1 -3.7 CG9466 CG9466 alpha mannosidase activity - sustained + -5.4 -8.4 -2.7 -3.8 CG9468 CG9468 alpha mannosidase activity - sustained + -4.8 -10 -2.5 -3 CG6206 CG6206 alpha mannosidase activity sustained -4.2 -5.8 -4.8 -4.3 CG11909 CG11909 alpha-glucosidase activity early -4 -1.4 -3.1 -1.6 CG1774 CG1774 hydrolase activity sustained -4 -2.2 -6 -3.8 CG5322 CG5322 alpha mannosidase activity sustained -3.7 -4.5 -4.9 -4.5 CG8693 CG8693 alpha glucosidase activity early -3.4 -1.8 -2.5 -1.2 LvpL CG8695 alpha glucosidase activity - sustained -3.3 -3.2 -1.9 -1.3 Gal CG9092 beta galactosidase activity sustained -3.3 -3.3 -1.8 -3.5 CG12120 CG12120 hydrolase activity sustained -2.9 -2.4 -2.8 -2.4 Amyrel CG8221 amylase activity - sustained -2.4 -4.3 1.7 -1.2 CG7997 CG7997 alpha-galactosidase activity early -2.1 -1.3 -1.9 -1.4 CG11669 CG11669 alpha-glucosidase activity + early -2.1 -1.4 -2.6 -4.7 CG32751 CG32751 hydrolase activity early -2 -1.1 -2 1.5 CG30360 CG30360 glucosidase activity early -2 -1 -1.7 -1.1 CG8768 CG8768 hydro-lyase activity - late 1.1 -2 25.9 12.3 CG3599 CG3599 hydrolase activity - late 1.2 -2 2.4 1.2 Lipase CG2772 CG2772 triacylglycerol lipase activity sustained -5.2 -6.3 -5.4 -4.1 CG31091 CG31091 triacylglycerol lipase activity - sustained -3.8 -8.4 -3 -3.2 CG31089 CG31089 triacylglycerol lipase activity - sustained -3.7 -3.2 -1.5 -4.1 CG17292 CG17292 triacylglycerol lipase activity early + -3.1 -1.7 -2.3 -1.6 CG5932 CG5932 triacylglycerol lipase activity sustained + -2.8 -4.3 -2.2 -3.1 CDase CG1471 lipase activity sustained -2.7 -2 -3.6 -1.7 CG3635 CG3635 lipase activity early -2.2 -1.6 -2.7 -1.9 CG5966 CG5966 triacylglycerol lipase activity early -2.1 -1.5 -1.9 -1.3 CG6271 CG6277 triacylglycerol lipase activity sustained -2 -6.3 -1.9 -3.2 CG6295 CG6295 phospholipase A1 activity late -1.6 -2.3 -1.2 -1.8 CG6283 CG6283 phospholipase A1 activity - late -1.6 -2.4 1 -1.1 CG17192 CG17192 phospholipase A1 activity late + -1.3 -3.6 1.1 -2.4 Miscealleanous CG8745 CG8745 alanine-glyoxylate transaminase activity sustained -6.6 -6.8 -4.9 -6.1 CG9471 CG9471 biliverdin reductase activity early -2.4 -1.2 -1.7 -1 Reg-2 CG3200 catalytic activity early -2.3 -1.7 -1.6 -1.3 CG16771 CG16771 alkaline phosphatase activity early -2.2 -1.6 -2.4 -1.5 CG32750 CG32750 pantetheinase activity sustained -2.2 -2.1 -2 -1.4 CG1809 CG1809 alkaline phosphatase activity sustained -2.2 -2.6 -2.3 -1.8 CG5150 CG5150 alkaline phosphatase activity late -1.8 -2.5 -1 -2.5 CG10592 CG10592 alkaline phosphatase activity late -1.6 -2.3 -1 -2.3 METABOLISM Carbohydrate metabolism CG32444 CG32444 aldose epimerase activity sustained -3.9 -4.3 -3 -5.1 CG3774 CG3774 nucleotide-sugar transmembrane transporter activity early -2.9 -1.6 -3 -1.5 CG10467 CG10467 aldose epimerase activity early + -2.5 -1.8 -1.9 -1.4 CG8690 CG8690 alpha glucosidase activity + early -2.3 -1.2 -4.4 -3.4 CG33080 CG33080 alpha glucosidase activity early -2.3 -1.7 -2 -2.1 CG6904 CG6904 glycogen (starch) synthase activity early -2.2 1 -1.4 1.2 CG9008 CG9008 aldose 1-epimerase activity early -2.1 -1.7 -2.1 -2.4 CG11594 CG11594 carbohydrate metabolic process late + -1.4 -2.4 -1.2 -1.5 Glucuronosyl-transferase activity Ugt37b1 CG9481 glucuronosyl-transferase activity - sustained -8.2 -4.2 -4.1 -2.7 Ugt86Dd CG6633 glucuronosyl-transferase activity sustained -7.6 -2.6 -9.1 -4 Ugt86Dh CG4772 glucuronosyl-transferase activity + early -3.9 -1.5 -7.1 -3.1 Ugt86Dg CG17200 glucuronosyl-transferase activity sustained -3.5 -3.6 -3.4 -3.2 Ugt86Dc CG4739 glucuronosyl-transferase activity early -2.9 -1.3 -2.8 -1.5 CG4302 CG4302 glucuronosyl-transferase activity early -2.6 -1.8 -3.8 -1.8 Ugt36Bc CG17932 glucuronosyl-transferase activity sustained -2.2 -2.5 -1.5 -1.4 CG5724 CG5724 glucuronosyl-transferase activity - sustained -2.2 -3.4 -1.2 -1.3 Ugt36Bb CG13271 glucuronosyl-transferase activity late -1.5 -2.2 -1.3 -1.8 Lipid metabolism CPTI CG12891 carnitine O-palmitoyltransferase activity early -4 -1.2 -4.7 -1.7 CG10877 CG10877 acyl transferase early -3.3 -1.7 -3.1 -2.1 CG12512 CG12512 long-chain-fatty-acid-CoA ligase activity sustained -3.1 -3.2 -1.6 -1.9 CG3961 CG3961 long-chain-fatty-acid-CoA ligase activity early -2.9 -1.7 -2.1 -1.8 LpR1 CG4861 low-density lipoprotein receptor activity early -2.6 -1.6 -1.3 -1.7 cue CG12086 low-density lipoprotein receptor activity early -2.6 -1.7 -1.7 -1.3 CG11162 CG11162 cholesterol metabolic process sustained -2.4 -4.4 -2.2 -5.6 Pde6 CG8279 3',5'-cyclic-GMP phosphodiesterase activity early -2.3 -1.3 -2.9 -1.9 Lip3 CG8823 triacylglycerol lipase activity late -1.9 -3.7 -1.3 -2.4 Acyl-coA metabolism CG9527 CG9527 acyl-coA dehydrogenase activity early + -2.9 -1.9 -2.4 -1.7 CG5009 CG5009 acyl-coA dehydrogenase activity sustained -2.3 -3 -1.7 -1.8 Acox57D-d CG9709 acyl-coA oxidase activity early -2.2 -1.5 -2.1 -2 Acox57D-p CG9707 acyl-CoA oxidase activity early -2.1 -1.8 -1.6 -1.6 CG13890 CG13890 dodecenoyl-CoA delta-isomerase activity early -2 -1.6 -2.2 -1.4 Amino acid and protein metabolism CG12338 CG12338 D-aspartate oxidase activity sustained -3.1 -2.3 -3.9 -2.9 CG9238 CG9238 protein phosphatase type 1 regulator activity sustained + -3.1 -2 -2.1 -1.3 Gs1 CG2718 glutamate synthase activity early -2.9 -1.2 -1.9 -1.2 CG10623 CG10623 methyltransferase activity sustained -2.8 -2 -3.1 -2.1 CG11236 CG11236 D-aspartate oxidase activity sustained + -2.5 -2.5 -2.2 -1.6 CG32486 CG32486 protein binding / ubiquitin dependant early -2.3 -1.3 -2.3 -1.6 CG8129 CG8129 L-threonine ammonia-lyase activity early + -2.3 -1.8 -3.8 -3 CG1753 CG1753 cystathionine beta-synthase activity early -2.2 -1.1 -1.9 1 CG5840 CG5840 pyrroline-5-carboxylate reductase activity early + -2.1 1 -2.4 -1.5 Nucleic acids metabolism RluA-1 CG31719 diaminohydroxyphosphoribosylaminopyrimidine deaminase activi - sustained -9.2 -3.1 -4 -1.9 CG1315 CG1315 argininosuccinate synthase activity sustained -4.7 -2.8 -3 -1.7 dhd CG4193 protein disulfide oxidoreductase activity + early -3.5 -1.4 -4 -2.8 CG8128 CG8128 nucleotide phosphatase activity early -3.3 -1.5 -2.9 -1.3 CG1961 CG1961 5'-nucleotidase activity early -2.6 -1.9 -2.5 -1.8 NitFhit CG7067 bis(5'-adenosyl)-triphosphatase activity early -2.5 -1.5 -2.5 -1.5 CG11126 CG11126 5'-nucleotidase activity sustained -2.2 -2.9 -2.6 -2.9 Other enzymes CG10621 CG10621 selenocysteine methyltransferase activity sustained + -5.3 -2.4 -4.1 -1.9 CG8112 CG8112 sterol o-acyl transferase activity - sustained -5.3 -3.1 -2.1 -1.7 CG7470 CG7470 delta1-pyrroline-5-carboxylate synthetase activity sustained -4.2 -2.4 -4.6 -2.6 CG9509 CG9509 choline dehydrogenase activity sustained -4 -3.6 -2.5 -2.2 Mipp1 CG4123 inositol phosphatase activity sustained -3.8 -2.3 -2.3 -1.3 CG11967 CG11967 carbonate dehydratase activity early -2.9 -1.4 -2.3 -1.2 CG10170 CG10170 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity + early -2.8 -1.6 -4 -3.7 CG2543 CG2543 tetrahydrofolylpolyglutamate synthase activity early -2.7 -1.6 -2.4 -1.4 CG30354 CG30354 ubiquinol-cytochrome-c reductase activity early -2.3 -1.2 -1.8 1.3 pug CG4067 formate-tetrahydrofolate ligase activity sustained -2.2 -2.1 -1.6 -1.1 Ahcy89E CG8956 adenosylhomocysteinase activity early -2 -1.5 -1.5 -1.2 Sodh-1 CG1982 L-iditol 2-dehydrogenase activity sustained + -2 -2.1 -1.4 -2.1 CG9512 CG9512 choline dehydrogenase activity - sustained -2 -2.5 1.5 -1.1 CG7678 CG7678 hydrogen-exporting ATPase activity + late -1.8 -2.1 -3.1 -4.3 FucTC CG40305 alpha(1,3)-fucosyltransferase activity - late -1.4 -2.6 15.5 7.2 Miscellaneaous Pdk CG8808 pyruvate metabolic process early -2.9 -1.6 -1.8 -1.2 CG4615 CG4615 cytolysis early -2.3 -1.5 -2.4 -1.1 CG17928 CG17928 heme binding sustained -2.3 -2.5 -2 -2.3 CG17036 CG17036 folic acid binding early -2.2 -1.4 -1.5 -1.1 CG33966 CG1211 vitamin E binding early -2 -1.2 -1.8 1 EPITHELIUM RENEWAL Cell cycle / Apoptosis ninaD CG31783 scavenger receptor activity sustained -8.9 -17.2 -8.9 -16.2 CG11299 CG11299 cell cycle arrest early -4.2 -1.4 -3.6 -2 CycB CG3510 cyclin-dependent protein kinase regulator activity early -2.6 1.1 -3.9 -2 Buffy CG8238 regulation of apoptosis early -2.4 -1.4 -2.2 -1.4 CG7227 CG7227 scavenger receptor activity sustained -2.2 -2 -2.1 -2.2 dap CG1772 cyclin-dependent protein kinase inhibitor activity early -2.1 -1.1 -2 -1.5 CG12789 CG12789 scavenger receptor activity late -1.5 -2.2 -1 -1.2 Cytoskeleton components Tina-1 CG2803 structural constituant of muscles early -2.8 -1.8 -2.8 -2.1 Dyb CG8529 structural constituent of cytoskeleton early -2.2 -1.1 -2.8 -1.9 Cell shape and adhesion mira CG12249 cytoskeletton / apico basal polarity sustained -3.3 -2 -3.8 -2.3 ltd CG8024 exocytosis early -2.2 -1.1 -2.2 -1.4 zpg CG10125 gap junction early -2.2 -1.5 -2.2 -1.6 mRNA localization nos CG5637 mRNA binding early -3 -1.6 -3.2 -2.2 osk CG10901 regulation of mRNA stability early -2.7 -1.2 -4.1 -1.9 BicC CG4824 mRNA binding / oskar negative regulation early -2.2 -1.5 -2.4 -1.7 Replication / Repair / Recombination RecQ5 CG4879 recombination early -2.4 -1.3 -2.4 -1.5 lok CG10895 DNA damage response early -2.1 -1.1 -2.4 -1.5 GENE EXPRESSION Transcription factor CG2120 CG2120 transcription regulation activity early -3.7 -1.6 -3.4 -2 sugarbabe CG3850 transcription activator activity + sustained -3.6 -2.1 -10.7 -3.7 CG6792 CG6792 transcription factor activity early -2.7 -1.4 -2.7 -2.2 bcd CG1034 transcription factor activity / morphogen early -2.6 -1.3 -2.9 -1.5 dsx CG11094 transcription factor activity early -2.5 -1.4 -1.8 -1.1 vnd CG6172 transcription factor activity early -2.2 -1.5 -1.9 -1.4 bun CG5461 transcription factor activity early -2.2 -1.6 -2.4 -1.9 bigmax CG3350 transcription factor activity early -2.2 -1.7 -2.6 -1.9 dve CG5799 transcription factor activity early -2.1 -1.1 -2 -1.2 CG18619 CG18619 transcription factor activity early -2.1 -1.5 -1.5 -1.5 CG7093 CG7093 transcription factor activity early -2.1 -1.6 -1.9 -1.4 foxo CG3143 transcription factor, insulin pathway early -2 -1.7 -2.3 -1.9 tsh CG1374 specific RNA polymerase II transcription factor activity late -1.8 -2.2 -1.9 -2.1 Chromatin regulator / DNA metabolism nocturnin CG31299 nucleic acid binding early -3.8 -1.7 -2.8 -1.4 CG13845 CG13845 chromatin assembly early + -3.5 -1.4 -3.4 -1.3 His2A CG31618 histones early -2.7 1.6 -4.1 -1.1 CG9793 CG9793 transcription regulator activity early -2.1 -1 -1.8 1.4 HmgZ CG17921 transcription regulator activity early -2 1.1 -1.4 1 CG5160 CG5160 transcription factor binding early -2 -1.3 -2.9 -2.5 CG4404 CG4404 DNA binding early -2 -1.4 -2.6 -1.8 HDAC6 CG6170 histone deacetylase activity sustained -2 -2.4 -1.7 -2.1 HmgD CG17950 AT DNA binding / regulation of transcription, DNA-dependent late -1.5 2.2 -1.6 1.3 CG7670 CG7670 3'-5' exonuclease activity late -1.1 3.2 -1.2 1.8 RNA regulation, splicing, protein regulation Paip2 CG12358 protein binding activity early -3.6 -1.5 -5.6 -2.7 CG10990 CG10990 translation elongation activity sustained -2.5 -2 -2.6 -1.8 CG11674 CG11674 ATP-dependent RNA helicase activity early -2 -1.2 -2 -1.6 RpS9 CG3395 structural constituent of ribosome early -2 -1.3 -2.3 -1.4 NEURONAL TRANSMISSION / HORMONES drl CG17348 olfactory learning / memory early -5 -1.9 -6.3 -2.7 CG13796 CG13796 sodium symporter activity early -4.1 -1.9 -3.9 -2.6 Eaat1 CG3747 sodium-dependent glutamate transporter activity - sustained -3.7 -3.2 -1.2 -1.5 slo CG10693 calcium activated potassium channel activity early -3 -1.9 -2.8 -1.7 Ork1 CG1615 potassium channel activity / locomotor rhythm early -2.7 -1.4 -2.7 -1.9 AR-2 CG10001 allostatin receptor activity early -2.5 -1.3 -2.3 -1.4 CG11340 CG11340 GABA receptor activity + early -2.4 -1.5 -3.1 -3.6 Jheh2 CG15102 Juvenile hormone epoxide hydrolase activity early -2.4 -1.6 -1.7 -1.1 KCNQ CG33135 voltage-gated potassium channel activity early -2.4 -1.6 -2.4 -1.8 Gs2 CG1743 Glutamate-ammonia ligase early -2.3 -1.4 -1.8 -1 shd CG13478 ecdysone 20-monooxygenase activity early -2.3 -1.7 -3 -1.8 vri CG14029 vrille early -2.3 -1.1 -2.6 -1.3 rogdi CG7725 olfactory memory early -2.2 -1.5 -2.1 -1.5 CG15534 CG15534 sphingomyelin phosphodiesterase activity - sustained + -2.1 -5.1 4.5 2.4 CG32843 CG32843 calcitonin receptor activity sustained -2.1 -2 -1.9 -2.1 Ace CG17907 acetylcholinesterase activity early -2 -1.5 -1.7 -1.7 for CG10033 cGMP-dependent protein kinase activity / feeding behaviour early -2 -1.3 -2.1 -1.4 tim CG3234 circadian rhythm early -2 -1.1 -2.2 -1.2 JhI-26 CG3767 target of juvenile hormone late -1.9 -3 -1.6 -2.9 UNKNOWN GENES CG15263 CG15263 unknown sustained + -26.1 -3.8 -30.2 -5.3 CG1946 CG1946 unknown sustained -11.9 -7.3 -10.6 -8.8 CG3934 CG3934 unknown sustained -11.8 -3.3 -8 -3.9 CG13360 CG13360 unknown early -10.6 -1.9 -13.2 -3.4 CG12813 CG12813 unknown sustained + -7.7 -9.2 -8.8 -7.8 CG7231 CG7231 unknown sustained -5.6 -3.1 -4.2 -2.2 CG18585 CG18585 unknown - sustained -5.6 -4.3 -4 -1.8 CG5973 CG5973 unknown sustained -5.5 -4.7 -5.4 -4.3 CG30272 CG30272 unknown - sustained -5.4 -3 -2.4 -1.6 CG13848 CG13848 unknown sustained -5.2 -3.6 -4.2 -2.7 CG13813 CG13813 unknown + sustained -5.1 -2 -12.5 -4.8 CG9005 CG9005 unknown sustained -5 -2 -3.9 -1.6 CG14615 CG14615 unknown sustained -4.7 -2.5 -4.2 -3 CG31104 CG31104 unknown - sustained -4 -2.3 -1.8 -1.4 CG31463 CG31463 unknown - sustained -3.7 -4.3 -2.4 -1.6 CG18577 CG18577 unknown - early -3.6 -1.4 -1.7 1.3 CG6908 CG6908 unknown + sustained + -3.6 -2.1 -11 -7.1 CG32352 CG32352 unknown early -3.5 -1.9 -2.7 -1.7 CG15209 CG15209 unknown sustained -3.5 -2 -3 -2 CG13255 CG13255 unknown sustained -3.5 -2.5 -3.6 -2.9 CG9555 CG9555 unknown sustained -3.4 -2.1 -2 -1.2 CG16854 CG16854 unknown early -3.3 -1.8 -3.3 -1.9 Kua CG10723 unknown early -3.2 -1.3 -3.5 -1.5 CG12643 CG12643 unknown early -3.2 -1.8 -3.8 -3.1 CG7916 CG7916 unknown sustained + -3.2 -2.1 -2.8 -1.8 CG11576 CG11576 unknown sustained -3.2 -2.2 -2.8 -1.6 CG3706 CG3706 unknown sustained -3 -2 -3 -1.6 CG8034 CG8034 unknown early -2.9 -1.6 -1.9 -1.1 CG7968 CG7968 unknown sustained -2.9 -2 -3.2 -1.5 CG9396 CG9396 unknown sustained -2.9 -2.3 -3 -2.9 CG15309 CG15309 unknown early -2.8 -1.8 -3 -1.9 CG30381 CG30381 unknown early -2.8 -1.8 -2.3 -1.8 CG7953 CG7953 unknown early -2.8 -1.9 -3.3 -1.9 CG17834 CG17834 unknown early -2.7 -1.8 -2.8 -2.3 CG15674 CG15674 unknown early -2.6 -1.1 -2.4 -1.1 CG31751 CG31751 unknown early -2.6 -1.4 -2.1 -1.4 CG10575 CG10575 unknown early -2.6 -1.6 -2.4 -1.7 CG33514 CG15017 unknown early -2.6 -1.6 -2.5 -1.5 CG9394 CG9394 unknown sustained -2.6 -2.1 -1.6 -1.3 CG13658 CG13658 unknown - sustained -2.6 -2.1 1.3 1.6 CG13202 CG13202 unknown sustained -2.6 -2.2 -2.3 -1.8 yellow-g CG5717 unknown early -2.5 1.3 -2.6 1.1 CG5151 CG5151 unknown early -2.5 -1.2 -3.6 -1.8 CG1942 CG1942 unknown early -2.5 -1.3 -1.8 1 CG5807 CG5807 unknown early -2.5 -1.3 -2.6 -1.4 CG12119 CG12119 unknown early -2.5 -1.3 -2 -1.3 CG10365 CG10365 unknown early -2.5 -1.5 -2.6 -1.6 CG9119 CG9119 unknown - early + -2.5 -1.6 -1.3 -1.4 CG6659 CG6659 unknown sustained -2.5 -2.1 -3.2 -2 CG12214 CG12214 unknown early -2.4 -1.3 -2.6 -1.5 CG18607 CG18607 unknown early -2.4 -1.5 -2 -1.2 CG13886 CG13886 unknown early -2.3 -1.6 -2.7 -1.9 CG34010 Dm.2L.7428.0 unknown early -2.3 -1.5 -2.2 -1.5 CG6830 CG6830 unknown sustained -2.3 -2.1 -2.6 -1.7 CG16723 CG16723 unknown - sustained -2.3 -2.2 -2.3 -1 CG17906 CG17906 unknown - sustained -2.3 -2.3 -1.7 1.1 CG14545 CG14545 unknown - early -2.3 -1 -1.9 2.5