DOCSLIB.ORG

55Th Annual W.W.O.A. Conference October 5-8, 2021 La Crosse Convention Center, La Crosse Inside This Issue… 2021- 2022 W.W.O.A

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
55Th Annual W.W.O.A. Conference October 5-8, 2021 La Crosse Convention Center, La Crosse Inside This Issue… 2021- 2022 W.W.O.A VOL. 241, JUNE 2021 WISCONSIN WASTEWATER OPERATORS’ ASSOCIATION, INC. Aerial view of Jefferson Wastewater Treatment Plant, Jefferson, Wisconsin 55th Annual W.W.O.A. Conference October 5-8, 2021 La Crosse Convention Center, La Crosse Inside This Issue… 2021- 2022 W.W.O.A. OFFICIAL DIRECTORY • Presidents message / Page 3 Don Lintner Jenny Pagel President Director (2021) N2511 State Rd 57 Wastewater Foreman • Tribute to Tim Nennig / Page 4 New Holstein, WI 53061 City of Clintonville Cell: 920-418-3869 N9055 Cty Road M [email protected] Shiocton WI 54170 • City of Jefferson Wastewater / Page 6 Work: 715-823-7675 Rick Mealy Cell: 920-606-4634 President Elect [email protected] Independent Contractor Lab • Board meeting minutes & Regulatory Assistance April 2 and 3, 2020 / Page 17 319 Linden Lane Marc Stephanie Delavan WI 53115 Director (2020) Cell: 608-220-9457 Director of Public Works • Collection System seminars / Page 24 [email protected] Village of Valders 1522 Puritan Rd New Holstein WI 53061 Jeremy Cramer Work: 920-629-4970 • Board meeting minutes Vice President Wastewater Treatment Cell: 920-251-8100 March 19, 2020 / Page 25 Director [email protected] City of Sun Prairie Joshua Voigt 300 E Main Street • Reminder: Director (2022) Sun Prairie WI 53590 Direct Sales Representative Awards nominations / Page 26 Work: 608-825-0731 Flygt a Xylem Brand Cell: 608-235-9280 3894 Lake Drive jcramer@ Hartford WI 53027 • Troubleshooting Corner: cityofsunprairie.com Work: 262-506-2343 Zoogloea and Thauera / Page 27 Cell: 414-719-5567 [email protected] Jeff Smudde • Index of advertisers / Page 30 Past President Nate Tillis Director of Environmental Director (2022) Programs Maintenance Supervisor NEW Water (GBMSD) City of Waukesha 2231 N Quincy St. 600 Sentry Drive Green Bay, WI 54302 Waukesha WI 53186 The Clarifier is the publication of the Work: 920-438-1040 Work: 262-524-3626 Wisconsin Wastewater Operators’ Association Cell: 920-851-1087 Cell: 414-507-1140 and is intended to inform and educate [email protected] [email protected] the membership on issues related to the treatment and control of wastewater. The Ben Brooks Courtney Harris Clarifier is produced five (5) times each Director (2021) Executive Secretary Superintendent Medford 7044 S 13th Street year: February, April, June, September, and Wastewater Treatment Fac Oak Creek, WI 53154 December. All members are encouraged to City of Medford Phone: 414-908-4950 contribute to the mission of the Clarifier. 603 S Whelen Avenue [email protected] Medford, WI 54451 The Wisconsin Wastewater Operators’ Work: 715-748-4122 Clarifier Staff Association is a non-profit organization Cell: 715-965-2206 Jon Butt 414-755-1149 [email protected] [email protected] dedicated to educating, informing, and advancing the wastewater profession. Ron Dickrell 715-387-1464 WWOA has approximately 2,000 members Cell: 715-897-2863 divided throughout six regions: Southeast, [email protected] Southern, Lake Michigan, North Central, Northwest, and West Central. Doralee Piering 262-377-6360 [email protected] The Clarifier JUNE 2021, VOL 241 3 Presidents Message: In person conference preparations I’m sitting at the picnic table without a jacket, so it looks Preparations are going forward for an in-person conference. like summer is finally here. Another great season to get out The website is being updated so some things are a little and explore and enjoy Wisconsin’s resources. It could be behind. By the time you read this more detailed conference something as simple as a game of golf with old friends. It information should be available on the website. Remember could be a slightly more challenging goal with people you to fill out nomination forms for a deserving person. love, like camping at unfamiliar locations. My wife and I have always liked short day hikes at parks around the Our regions are full of people that have done something state, now we’re setting a goal to hike the entire Ice Age outstanding or innovative. Perhaps they have “just” quietly worked to keep their facilities operating well day in and day out to serve the community and protect the waters that we all rely on. This is the opportunity to recognize their contributions. Regional Operator of the Year Award nominations should be submitted to your regional officers by July 24, 2021. All other awards need to be submitted to Rick Mealy, the WWOA Awards Committee Chair by August 1, 2021. I was saddened to hear of the passing of Past President Tim Nennig. Although I served with Tim on a DNR advisory committee many years ago, I never got the opportunity to know him really well. He seemed like a good person and I respected his knowledge and opinions. I always thought of him as a good example of what dedication to our profession and involvement in WWOA should look like. My prayers go out to his family, he will be missed. I’ll close this the way that I did in my last message. Keep doing the things that you do to protect the waters of Wisconsin so we can enjoy them all year long. Take Care Everyone, Don Lintner National Scenic Trail. It’s 1200 miles of trails and connecting routes located entirely in Wisconsin, featuring the beautiful landscapes that the glaciers gave us. Remaining As I’m writing this, the conference in La Crosse is only 5 Clarifier months away. It’s hard to believe that my term as president is Deadlines more than half over. With things being the way that they are I regret not being able to get around the state, meeting more September Issue: of you and learning about your projects during my term. August 13 That can change now that people are getting vaccinated and mandates are being loosened. The WWOA Board has made December Issue: the decision to allow regional meetings to be in person November 12 following local guidelines. Might not be the perfect situation yet, but we’re getting there. 4 VOL 241, JUNE 2021 The Clarifier Tim Nennig Tim Nennig, a life-time member and a past president of WWOA, passed away April 23, 2021 at the age of 59. Tim left us much too early and our WWOA community is not the same now that he is gone. Tim married his high school sweetheart, Holly and was the proud father of three children, Bailey, Sadie, and Alex. I first met Tim through a mutual friend outside of WWOA. Tim’s daughter and my friend’s daughter were on the same swim team. As we talked, we realized that we were in the same “profession”; a fraternity of individuals committed to improving the world around us one wastewater treatment plant at a time. It was a bit later, 2011 to be exact, when our professional worlds would join as I shared with Tim an idea on the Milwaukee River that he embraced. And for the next 10 years, we pursued a shared vision to see if we could make a difference. Tim, like many of us, was very proud of the work he did. He served the Village of Grafton very well as evidenced by the outstanding performance of the treatment plant and the level of respect he received from those around him. I would classify Tim as part of the “old guard”. He served WWOA on the board and then later as president many years ago. He never wavered in his support for the organization and was always willing to back anyone who wished to present on something the Village was doing. It was at the 51st Annual WWOA conference when Tim’s life would forever change. He told me it was at that conference when he experienced pain like he never had before during the awards banquet. He ended up in the hospital where multiple tests were run. The source of his pain was discovered but it would take a bit more time to reveal the true cause of Tim’s health problem. If we have learned anything over the past year is that life is short. Tim set an example of what to do with the life you have. I never heard him complain even when he had every right to. He embraced life, he tried to make things better, he set an example that we can all strive for. I will miss Tim. My life is better for having had the chance to work with him. I suspect many of us can say the same. Jon Butt The Clarifier JUNE 2021, VOL 241 5 CLOGGED PUMPS? Tackle wipes and large debris with these solutions from USABlueBook! FOR SMALLER APPLICATIONS OPERATOR AND LIFT STATIONS DESIGNED Goulds GFK and GFV Series Sewage Pumps • Non-clog impellers handle wipes and 2" solids FlowRake Rag Catchers • Catch and retain flushables EXCLUSIVE FOR LARGE MUNICIPAL LIFT STATIONS Deming Demersible Chopper Pumps • Slice the most troublesome solids into small pieces StationGuard Manual OPERATOR Bar Screens DESIGNED • Capture damaging wipes and debris GET PRODUCT DETAILS AT usabluebook.com/NoMoreClogs 800.548.1234 • usabluebook.com Wipe_Control_Ad_7.5x9.5.indd 1 4/20/21 9:01 AM 6 VOL 241, JUNE 2021 The Clarifier City of Jefferson wastewater treatment facility Jefferson, Wisconsin is a quiet Wisconsin community that sits at the confluence of the Rock and Crawfish Rivers. Main Street shows off its vintage buildings and steepled churches built by German craftsmen. Located thirty minutes east of Madison and forty-five minutes west of Milwaukee, it provides the small-town atmosphere with access to big city amenities. The treatment plant serves a population of just under 8,000 and has several commercial and industrial customers.
Load more

Recommended publications
  • The 2014 Golden Gate National Parks Bioblitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event
    National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 ON THIS PAGE Photograph of BioBlitz participants conducting data entry into iNaturalist. Photograph courtesy of the National Park Service. ON THE COVER Photograph of BioBlitz participants collecting aquatic species data in the Presidio of San Francisco. Photograph courtesy of National Park Service. The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 Elizabeth Edson1, Michelle O’Herron1, Alison Forrestel2, Daniel George3 1Golden Gate Parks Conservancy Building 201 Fort Mason San Francisco, CA 94129 2National Park Service. Golden Gate National Recreation Area Fort Cronkhite, Bldg. 1061 Sausalito, CA 94965 3National Park Service. San Francisco Bay Area Network Inventory & Monitoring Program Manager Fort Cronkhite, Bldg. 1063 Sausalito, CA 94965 March 2016 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service.
    [Show full text]
  • Thiobacillus Denitrificans
    Nitrate-Dependent, Neutral pH Bioleaching of Ni from an Ultramafic Concentrate by Han Zhou A thesis submitted in conformity with the requirements for the degree of Master of Applied Science Chemical Engineering and Applied Chemistry University of Toronto © Copyright by Han Zhou 2014 ii Nitrate-Dependent, Neutral pH Bioleaching of Ni from an Ultramafic Concentrate Han Zhou Master of Applied Science Chemical Engineering and Applied Chemistry University of Toronto 2014 Abstract This study explores the possibility of utilizing bioleaching techniques for nickel extraction from a mixed sulfide ore deposit with high magnesium content. Due to the ultramafic nature of this material, well-studied bioleaching technologies, which rely on acidophilic bacteria, will lead to undesirable processing conditions. This is the first work that incorporates nitrate-dependent bacteria under pH 6.5 environments for bioleaching of base metals. Experiments with both defined bacterial strains and indigenous mixed bacterial cultures were conducted with nitrate as the electron acceptor and sulfide minerals as electron donors in a series of microcosm studies. Nitrate consumption, sulfate production, and Ni released into the aqueous phase were used to track the extent of oxidative sulfide mineral dissolution; taxonomic identification of the mixed culture community was performed using 16S rRNA gene sequencing. Nitrate-dependent microcosms that contained indigenous sulfur- and/or iron-oxidizing microorganisms were cultured, characterized, and provided a proof-of-concept basis for further bioleaching studies. iii Acknowledgments I would like to extend my most sincere gratitude toward both of my supervisors Dr. Vladimiros Papangelakis and Dr. Elizabeth Edwards. This work could not have been completed without your brilliant and patient guidance.
    [Show full text]
  • Community and Whole Genome Analysis of Wastewater Bacteria
    Community and Whole Genome Analysis of Wastewater Bacteria By Daniel Rice BSc(Hons) A thesis submitted in total fulfilment of the requirements for the degree of Master of Science School of Life Sciences College of Science, Health and Engineering La Trobe University Victoria Australia February 2020 1 TABLE OF CONTENTS TABLE OF CONTENTS ................................................................................................................ i LIST OF FIGURES ..................................................................................................................... iii LIST OF TABLES ....................................................................................................................... iv LIST OF ABBREVIATIONS .......................................................................................................... v STATEMENT OF AUTHORSHIP ................................................................................................vii ACKNOWLEDGEMENTS ......................................................................................................... viii SUMMARY ..............................................................................................................................ix SECTION 1: INTRODUCTION 1.1 Activated sludge process ................................................................................................... 2 1.2 Community profiling of activated sludge bacterial communities ....................................... 7 1.2.1 Phosphorous removal ..........................................................................................
    [Show full text]
  • Alpine Soil Bacterial Community and Environmental Filters Bahar Shahnavaz
    Alpine soil bacterial community and environmental filters Bahar Shahnavaz To cite this version: Bahar Shahnavaz. Alpine soil bacterial community and environmental filters. Other [q-bio.OT]. Université Joseph-Fourier - Grenoble I, 2009. English. tel-00515414 HAL Id: tel-00515414 https://tel.archives-ouvertes.fr/tel-00515414 Submitted on 6 Sep 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE Pour l’obtention du titre de l'Université Joseph-Fourier - Grenoble 1 École Doctorale : Chimie et Sciences du Vivant Spécialité : Biodiversité, Écologie, Environnement Communautés bactériennes de sols alpins et filtres environnementaux Par Bahar SHAHNAVAZ Soutenue devant jury le 25 Septembre 2009 Composition du jury Dr. Thierry HEULIN Rapporteur Dr. Christian JEANTHON Rapporteur Dr. Sylvie NAZARET Examinateur Dr. Jean MARTIN Examinateur Dr. Yves JOUANNEAU Président du jury Dr. Roberto GEREMIA Directeur de thèse Thèse préparée au sien du Laboratoire d’Ecologie Alpine (LECA, UMR UJF- CNRS 5553) THÈSE Pour l’obtention du titre de Docteur de l’Université de Grenoble École Doctorale : Chimie et Sciences du Vivant Spécialité : Biodiversité, Écologie, Environnement Communautés bactériennes de sols alpins et filtres environnementaux Bahar SHAHNAVAZ Directeur : Roberto GEREMIA Soutenue devant jury le 25 Septembre 2009 Composition du jury Dr.
    [Show full text]
  • Supplementary Information for Microbial Electrochemical Systems Outperform Fixed-Bed Biofilters for Cleaning-Up Urban Wastewater
    Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology. This journal is © The Royal Society of Chemistry 2016 Supplementary information for Microbial Electrochemical Systems outperform fixed-bed biofilters for cleaning-up urban wastewater AUTHORS: Arantxa Aguirre-Sierraa, Tristano Bacchetti De Gregorisb, Antonio Berná, Juan José Salasc, Carlos Aragónc, Abraham Esteve-Núñezab* Fig.1S Total nitrogen (A), ammonia (B) and nitrate (C) influent and effluent average values of the coke and the gravel biofilters. Error bars represent 95% confidence interval. Fig. 2S Influent and effluent COD (A) and BOD5 (B) average values of the hybrid biofilter and the hybrid polarized biofilter. Error bars represent 95% confidence interval. Fig. 3S Redox potential measured in the coke and the gravel biofilters Fig. 4S Rarefaction curves calculated for each sample based on the OTU computations. Fig. 5S Correspondence analysis biplot of classes’ distribution from pyrosequencing analysis. Fig. 6S. Relative abundance of classes of the category ‘other’ at class level. Table 1S Influent pre-treated wastewater and effluents characteristics. Averages ± SD HRT (d) 4.0 3.4 1.7 0.8 0.5 Influent COD (mg L-1) 246 ± 114 330 ± 107 457 ± 92 318 ± 143 393 ± 101 -1 BOD5 (mg L ) 136 ± 86 235 ± 36 268 ± 81 176 ± 127 213 ± 112 TN (mg L-1) 45.0 ± 17.4 60.6 ± 7.5 57.7 ± 3.9 43.7 ± 16.5 54.8 ± 10.1 -1 NH4-N (mg L ) 32.7 ± 18.7 51.6 ± 6.5 49.0 ± 2.3 36.6 ± 15.9 47.0 ± 8.8 -1 NO3-N (mg L ) 2.3 ± 3.6 1.0 ± 1.6 0.8 ± 0.6 1.5 ± 2.0 0.9 ± 0.6 TP (mg
    [Show full text]
  • Bioflocculation of Wastewater Organic Matter at Short Retention Times
    Bioflocculation of Wastewater Organic Matter at Short Retention Times Lena Faust Thesis committee Promotor Prof. dr. ir. H.H.M. Rijnaarts Professor, Chair Environmental Technology Wageningen University Co-promotor Dr. ir. H. Temmink Assistant professor, Sub-department of Environmental Technology Wageningen University Other members Prof. Dr A.J.M. Stams, Wageningen University Prof. Dr. I. Smets, University of Leuven, Belgium Prof. Dr. B.-M. Wilen, Chalmers University of Technology, Sweden Prof. Dr. D.C. Nijmeijer, University of Twente, The Netherlands This research was conducted under the auspices of the Graduate School SENSE (Socio- Economic and Natural Sciences of the Environment) Bioflocculation of Wastewater Organic Matter at Short Retention Times Lena Faust Thesis submitted in fulfillment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr M.J. Kropff, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Wednesday 3rd of December 2014 at 1.30 p.m. in the Aula. Lena Faust Bioflocculation of Wastewater Organic Matter at Short Retention Times, 163 pages. PhD thesis, Wageningen University, Wageningen, NL (2014) With references, with summaries in English and Dutch ISBN 978-94-6257-171-6 ˮDa steh ich nun, ich armer Tor, und bin so klug als wie zuvor.ˮ Dr. Faust (in Faust I written by Johann Wolgang von Goethe) Contents 1 GENERAL INTRODUCTION ....................................................................................................................1
    [Show full text]
  • DGGE) and PGR Cloning of 16S Rrna Genes
    THE UNIVERSITY OF NEW SOUTH thesis/Dissertation Sheet Surname or Family name:LE Rrst ^ I Other narne/s: Abbreviation for degree as given in the University calendar: MSc | ScliooliBiotechnolo^^^^^^ and Biomolecular ScienoBS Faculty: Science Title:Community analysis and physiological characterisation of bacterial isolates from a nitrifying membrane bioreactor Abstract This thesis focuses on the identification of early colonisers on membrane surfaces used in wastewater treatment, as well as the physiological characterisation of bacterial cultures isolated from different micro- environments of a membrane bioreactor (MBR). The bacterial community composition of early biofilms on membrane surfaces under different hydrodynamic conditions (pressurised and non-pressurised) and of the activated sludge in an MBR were examined by culture-independent, molecular-based methods of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and PGR cloning of 16S rRNA genes. A bench-scale, nitrifying MBR treating artificial waste was employed. The hollow fibre ultrafiltration membrane was made of polypropylene with an average pore diameter of 0.04 \im. Analysis of DGGE profiles of the sessile communities on membrane surfaces revealed that Tetrasphaera elongata species were important colonisers due to their ability to bind to membrane surfaces irrespective of the hydrodynamic context and exposure time. Interactions between isolates from the bioreactor and membrane surfaces were further investigated by characterising the physiological traits important in biofilm initiation and proliferation on membrane surfaces such as motility, auto-aggregation, co-aggregation, hydrophobicity and quorum sensing. Bacterial strains were isolated from floes and supernatant phases of the activated sludge as well as from pressurised membrane surfaces. Microbacterium sp. were prevalent in all culture collections.
    [Show full text]
  • Methylated Amine-Utilising Bacteria and Microbial Nitrogen Cycling in Movile Cave
    METHYLATED AMINE-UTILISING BACTERIA AND MICROBIAL NITROGEN CYCLING IN MOVILE CAVE DANIELA WISCHER UNIVERSITY OF EAST ANGLIA PHD 2014 METHYLATED AMINE-UTILISING BACTERIA AND MICROBIAL NITROGEN CYCLING IN MOVILE CAVE A thesis submitted to the School of Environmental Sciences in fulfilment of the requirements for the degree of Doctor of Philosophy by DANIELA WISCHER in SEPTEMBER 2014 University of East Anglia, Norwich, UK This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with the author and that use of any information derived there from must be in accordance with current UK Copyright Law. In addition, any quotation or extract must include full attribution. i Table of Contents List of Figures ............................................................................................................... vii List of Tables .................................................................................................................. xi Declaration....................................................................................................................xiii Acknowledgements ...................................................................................................... xiv Abbreviations & Definitions ......................................................................................... xv Abstract........ ................................................................................................................ xix Chapter 1. Introduction
    [Show full text]
  • Isotope Array Analysis of Rhodocyclales Uncovers Functional Redundancy and Versatility in an Activated Sludge
    The ISME Journal (2009) 3, 1349–1364 & 2009 International Society for Microbial Ecology All rights reserved 1751-7362/09 $32.00 www.nature.com/ismej ORIGINAL ARTICLE Isotope array analysis of Rhodocyclales uncovers functional redundancy and versatility in an activated sludge Martin Hesselsoe1, Stephanie Fu¨ reder2, Michael Schloter3, Levente Bodrossy4, Niels Iversen1, Peter Roslev1, Per Halkjær Nielsen1, Michael Wagner2 and Alexander Loy2 1Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark; 2Department of Microbial Ecology, University of Vienna, Wien, Austria; 3Department of Terrestrial Ecogenetics, Helmholtz Zentrum Mu¨nchen—National Research Center for Environmental Health, Neuherberg, Germany and 4Department of Bioresources/Microbiology, ARC Seibersdorf Research GmbH, Seibersdorf, Austria Extensive physiological analyses of different microbial community members in many samples are difficult because of the restricted number of target populations that can be investigated in reasonable time by standard substrate-mediated isotope-labeling techniques. The diversity and ecophysiology of Rhodocyclales in activated sludge from a full-scale wastewater treatment plant were analyzed following a holistic strategy based on the isotope array approach, which allows for a parallel functional probing of different phylogenetic groups. Initial diagnostic microarray, comparative 16S rRNA gene sequence, and quantitative fluorescence in situ hybridization surveys indicated the presence of a diverse community, consisting of an estimated number of 27 operational taxonomic units that grouped in at least seven main Rhodocyclales lineages. Substrate utilization profiles of probe-defined populations were determined by radioactive isotope array analysis and microautoradiography-fluorescence in situ hybridization of activated sludge samples that were briefly exposed to different substrates under oxic and anoxic, nitrate-reducing conditions.
    [Show full text]
  • First Draft Genome Sequence of a Strain Belonging to the Zoogloea Genus and Its Gene Expression in Situ Emilie E
    Muller et al. Standards in Genomic Sciences (2017) 12:64 DOI 10.1186/s40793-017-0274-y EXTENDED GENOME REPORT Open Access First draft genome sequence of a strain belonging to the Zoogloea genus and its gene expression in situ Emilie E. L. Muller1,3†, Shaman Narayanasamy1†, Myriam Zeimes1, Cédric C. Laczny1,4, Laura A. Lebrun1, Malte Herold1, Nathan D. Hicks2, John D. Gillece2, James M. Schupp2, Paul Keim2 and Paul Wilmes1* Abstract The Gram-negative beta-proteobacterium Zoogloea sp. LCSB751 (LMG 29444) was newly isolated from foaming activated sludge of a municipal wastewater treatment plant. Here, we describe its draft genome sequence and annotation together with a general physiological and genomic analysis, as the first sequenced representative of the Zoogloea genus. Moreover, Zoogloea sp. gene expression in its environment is described using metatranscriptomic data obtained from the same treatment plant. The presented genomic and transcriptomic information demonstrate a pronounced capacity of this genus to synthesize poly-β-hydroxyalkanoate within wastewater. Keywords: Genome assembly, Genomic features, Lipid metabolism, Metatranscriptomics, Poly-hydroxyalkanoate, Wastewater treatement plant Introduction Zoogloea species and thus, limited information is avail- Zoogloea spp. are chemoorganotrophic bacteria often able with regards to the genomic potential of the genus. found in organically enriched aquatic environments and Here we report the genome of a newly isolated Zoogloea are known to be able to accumulate intracellular gran- sp. strain as a representative of the genus, with a focus ules of poly-β-hydroxyalkanoate [1]. The combination of on its biotechnological potential in particular for the these two characteristics renders this genus particulary production of biodiesel or bioplastics.
    [Show full text]
  • UC Irvine Electronic Theses and Dissertations
    UC Irvine UC Irvine Electronic Theses and Dissertations Title Understanding of Nitrifying and Denitrifying Bacterial Population Dynamics in an Activated Sludge Process Permalink https://escholarship.org/uc/item/1bd53495 Author Wang, Tongzhou Publication Date 2014 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA, IRVINE DISSERTATION Submitted in Partial Satisfaction of the Requirements for the Degree of DOCTOR OF PHILOSOPHY in Engineering by Tongzhou Wang Dissertation Committee Professor Betty H. Olson, Chair Professor Diego Rosso Professor Sunny C. Jiang 2014 © 2014 Tongzhou Wang ii To My Family ii Table of Contents Page LIST OF FIGURES .................................................................................................................. ix LIST OF TABLES .................................................................................................................... xi ACKNOWLEDGEMENTS ..................................................................................................... xii CURRICULUM VITAE ......................................................................................................... xiv ABSTRACT OF THE DISSERTATION ............................................................................... xvii Chapter 1. ................................................................................................................................ Introduction ...................................................................................................................................................
    [Show full text]
  • Universitá Di Bologna MICROBIAL ECOLOGY of BIOTECHNOLOGICAL PROCESSES
    Alma Mater Studiorum – Universitá di Bologna Dottorato di Ricerca in Scienze Biochimiche e Biotecnologiche Ciclo XXVII Settore Concorsuale 03/D1 Settore Scientifico Disciplinare CHIM/11 MICROBIAL ECOLOGY OF BIOTECHNOLOGICAL PROCESSES Presentata da: Dott.ssa Serena Fraraccio Coordinatore Dottorato Relatore Chiar.mo Prof. Chiar.mo Prof. Fabio Fava Santi Mario Spampinato Correlatori Giulio Zanaroli, Ph.D. Assoc. Prof. Ondřej Uhlík, Ph.D. Esame finale anno 2015 Abstract The investigation of phylogenetic diversity and functionality of complex microbial communities in relation to changes in the environmental conditions represents a major challenge of microbial ecology research. Nowadays, particular attention is paid to microbial communities occurring at environmental sites contaminated by recalcitrant and toxic organic compounds. Extended research has evidenced that such communities evolve some metabolic abilities leading to the partial degradation or complete mineralization of the contaminants. Determination of such biodegradation potential can be the starting point for the development of cost effective biotechnological processes for the bioremediation of contaminated matrices. This work showed how metagenomics-based microbial ecology investigations supported the choice or the development of three different bioremediation strategies. First, PCR-DGGE and PCR-cloning approaches served the molecular characterization of microbial communities enriched through sequential development stages of an aerobic cometabolic process for the treatment of groundwater
    [Show full text]