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DNMT1 Polyclonal Antibody Catalog Number:24206-1-AP Featured Product 43 Publications

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DNMT1 Polyclonal Antibody Catalog Number:24206-1-AP Featured Product 43 Publications For Research Use Only DNMT1 Polyclonal antibody Catalog Number:24206-1-AP Featured Product 43 Publications www.ptglab.com Catalog Number: GenBank Accession Number: Purification Method: Basic Information 24206-1-AP BC126227 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 850 μg/ml by 1786 WB 1:200-1:1000 Nanodrop and 407 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; DNA (cytosine-5-)-methyltransferase for WB Source: 1 IHC 1:50-1:500 IF 1:10-1:100 Rabbit Calculated MW: Isotype: 1632 aa, 185 kDa IgG Observed MW: Immunogen Catalog Number: 180-200 kDa AG19116 Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : HEK-293 cells, C6 cells Cited Applications: IP : HEK-293 cells, CoIP, IF, IHC, WB IHC : human lung cancer tissue, human breast cancer Species Specificity: tissue human, rat Cited Species: IF : HepG2 cells, Chicken, goat, human, pig, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA Background Information methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MV 184 kDa and 145 kDa. (PMID: 10753866) Notable Publications Author Pubmed ID Journal Application Yineng Han 32890413 Oral Dis WB Kalaiselvi Sivalingam 32899583 Brain Sci WB,IF Huihui Zeng 32883320 Respir Res WB Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data IP Result of anti-DNMT1 (IP:24206-1-AP, 5ug; Immunohistochemical analysis of paraffin- HEK-293 cells were subjected to SDS PAGE Detection:24206-1-AP 1:500) with HEK-293 cells embedded human lung cancer tissue slide using followed by western blot with 24206-1-AP (DNMT1 lysate 2500ug. 24206-1-AP (DNMT1 antibody) at dilution of 1:200 Antibody) at dilution of 1:300 incubated at room (under 40x lens). Heat mediated antigen retrieval temperature for 1.5 hours. with Tris-EDTA buffer (pH 9.0). Immunofluorescent analysis of HepG2 cells using 24206-1-AP (DNMT1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG..
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    The Year of the Rat Why Were Different Animals Chosen? Each new year in Chinese culture is known by an animal. There are 12 different animals altogether. This tradition began a very long time ago and is thought to have started with a story about Buddha. Buddha asked all of the animals to come and see him one day but only 12 animals came. Buddha gave each one of these animals their own year in the Chinese zodiac cycle to say thank you. When Does the Year of the Rat Start? The Year of the Rat starts on 25th January 2020. Once it is over, the Year of the Rat will only begin again in 2032. What Is Good about the Year of the Rat? There are a lot of good things about being born in the Year of the Rat. If someone is born in the Year of the Rat, Chinese culture believes that they will be: • adaptable – they will be able to adjust to changes; • smart – they will be clever; • cautious – they will be careful; • alert – they will always be on the lookout for danger; • a positive person; • someone who is friendly and confident; • someone who is always cheerful. What Jobs Would People Be Good At? Chinese culture believes that someone born in the Year of the Rat might make a good: • artist; • author; • teacher; • architect; • musician. Page 1 of 2 visit twinkl.com The Year of the Rat You were born in the Year of the Rat if you were born in: 1960 1972 1984 1996 2008 Did You Know...? If you were born in the Year of the Rat: • your lucky colours are gold, blue and green; • your lucky flowers are lilies and violets; • your lucky numbers are 2 and 3; • you will find love in the west and wealth in the east; • 2020 is predicted to be a year of opportunity.
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